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https://www.readbyqxmd.com/read/28538649/attenuation-of-hemorrhage-associated-lung-injury-by-adjuvant-treatment-with-c23-an-oligopeptide-derived-from-cold-inducible-rna-binding-protein-cirp
#1
Fangming Zhang, Weng-Lang Yang, Max Brenner, Ping Wang
BACKGROUND: Hemorrhagic shock (HS) is an important cause of mortality. HS is associated with an elevated incidence of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), significantly contributing to HS morbidity and mortality. Cold-inducible RNA-binding protein (CIRP) is released into the circulation during HS and can cause lung injury. C23 is a CIRP-derived oligopeptide that binds with high affinity to the CIRP receptor and inhibits CIRP-induced phagocyte secretion of TNF-α...
May 22, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28515455/comparative-genomic-analysis-reveals-the-environmental-impacts-on-two-arcticibacter-strains-including-sixteen-sphingobacteriaceae-species
#2
Liang Shen, Yongqin Liu, Baiqing Xu, Ninglian Wang, Huabiao Zhao, Xiaobo Liu, Fei Liu
How the genomic diversity of species is driven by geographical isolation and environmental factors are not well understood for cold environments. Here, the environmental stress responses of two phylogenetically close Arcticibacter strains, A. eurypsychrophilus MJ9-5 and A. svalbardensis MN12-7, isolated from a Tibetan Plateau glacier and Svalbard soil, were analyzed. The comparative genomic analysis was performed with sixteen other related Sphingobacteriaceae species. Analyses of the relationships between growth temperature and genome composition, cold and heat shock genes showed that genomic adaption characteristics were more obvious when the strains were grouped by their upper limit in growth temperature, rather than by their minimal or optimal growth temperatures for Sphingobacteriaceae species...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28500590/autolysis-of-pichia-pastoris-induced-by-cold
#3
Yaneth Bartolo-Aguilar, Luc Dendooven, Cipriano Chávez-Cabrera, Luis B Flores-Cotera, María E Hidalgo-Lara, Lourdes Villa-Tanaca, Rodolfo Marsch
The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes in the yeast after the growth stage could reduce protein recovery costs. This study shows that a cold-shock can be applied to induce lysis of the yeast cells. A strain of P. pastoris was constructed in which the endogenous eng gene encoding a putative endo-β-1,3-glucanase was overexpressed using the cold-shock induced promoter of the cctα gene from Saccharomyces cerevisiae...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28500030/translating-divergent-environmental-stresses-into-a-common-proteome-response-through-hik33-in-a-model-cyanobacterium
#4
Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, Yingchun Wang
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by Hik33 remains to be addressed. Using a hik33-deficient strain (Δhik33) of Synechocystis sp. PCC 6803 (Synechocystis) and quantitative proteomics, we found that Hik33 depletion induces differential protein expression highly similar to that induced by divergent types of stresses. This typically includes downregulation of proteins in photosynthesis and carbon assimilation that are necessary for cell propagation, and upregulation of heat shock proteins, chaperons, and proteases that are important for cell survival...
May 12, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28482796/effects-of-cold-acclimation-on-gene-expression-in-fall-field-cricket-gryllus-pennsylvanicus-ionoregulatory-tissues
#5
Lauren E Des Marteaux, Alexander H McKinnon, Hiroko Udaka, Jantina Toxopeus, Brent J Sinclair
BACKGROUND: Cold tolerance is a key determinant of temperate insect distribution and performance. Chill-susceptible insects lose ion and water homeostasis during cold exposure, but prior cold acclimation improves both cold tolerance and defense of homeostasis. The mechanisms underlying these processes are mostly unknown; cold acclimation is thought to enhance ion transport in the cold and/or prevent leak of water and ions. To identify candidate mechanisms of cold tolerance plasticity we generated transcriptomes of ionoregulatory tissues (hindgut and Malpighian tubules) from Gryllus pennsylvanicus crickets and compared gene expression in warm- and cold-acclimated individuals...
May 8, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28482045/using-gcamp3-to-study-ca2-signaling-in-nicotiana-species
#6
Thomas A DeFalco, Masatsugu Toyota, Van Phan, Purva Karia, Wolfgang Moeder, Simon Gilroy, Keiko Yoshioka
Ca2+ signaling is a central component of plant biology; however, direct analysis of in vivo Ca2+ levels is experimentally challenging. In recent years the use of genetically-encoded Ca2+ indicators have revolutionized the study of plant Ca2+ signaling, although such studies have been largely restricted to the model plant Arabidopsis. We have developed stable transgenic Nicotiana benthamiana and Nicotiana tabacum lines expressing the single-wavelength fluorescent Ca2+ indicator, GCaMP3. Ca2+ levels in these plants can be imaged in situ using fluorescence microscopy, and these plants can be used to both qualitatively and semi-quantitatively evaluate Ca2+ signals in response to a broad array of abiotic or biotic stimuli, such as cold-shock or pathogen-associated molecular patterns (PAMPs)...
May 8, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28477153/nitrogen-removal-performance-and-operation-strategy-of-anammox-process-under-temperature-shock
#7
Weiqiang Zhu, Jin Li, Huiyu Dong, Dan Wang, Peiyu Zhang
Sequencing batch reactors were used to study anaerobic ammonium oxidation (anammox) process under temperature shock. Both long-term (15-35 °C) and short-term (10-50 °C) temperature effects on nitrogen removal performance were performed. In reactor operation test, the results indicated that ammonium removal rate decreased from 0.35 kg/(m(3) day) gradually to 0.059 kg/(m(3) day) when temperature dropped from 35 to 15 °C. Although bacteria morphology was not modified, sludge settling velocity decreased with decreasing temperature...
May 5, 2017: Biodegradation
https://www.readbyqxmd.com/read/28473807/fnrl-and-three-dnr-regulators-are-used-for-the-metabolic-adaptation-to-low-oxygen-tension-in-dinoroseobacter-shibae
#8
Matthias Ebert, Sebastian Laaß, Andrea Thürmer, Louisa Roselius, Denitsa Eckweiler, Rolf Daniel, Elisabeth Härtig, Dieter Jahn
The heterotrophic marine bacterium Dinoroseobacter shibae utilizes aerobic respiration and anaerobic denitrification supplemented with aerobic anoxygenic photosynthesis for energy generation. The aerobic to anaerobic transition is controlled by four Fnr/Crp family regulators in a unique cascade-type regulatory network. FnrL is utilizing an oxygen-sensitive Fe-S cluster for oxygen sensing. Active FnrL is inducing most operons encoding the denitrification machinery and the corresponding heme biosynthesis. Activation of gene expression of the high oxygen affinity cbb3-type and repression of the low affinity aa3-type cytochrome c oxidase is mediated by FnrL...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28470617/cold-shock-expression-system-in-e-coli-for-protein-nmr-studies
#9
Toshihiko Sugiki, Toshimichi Fujiwara, Chojiro Kojima
The cold-shock system using the pCold vector is one of the most effective Escherichia coli heterologous protein expression systems. It allows the improvement of the expression level of the protein of interest in a soluble fraction. In this chapter, we describe practical procedures for the overexpression of heterologous protein of interest by using the pCold vector or the single-protein production system. The latter is one of the most advanced pCold technologies for isotope labeling of the target protein and its NMR studies...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28469602/ppmid1-plays-a-role-in-the-asexual-development-and-virulence-of-phytophthora-parasitica
#10
Fang-Yu Hwu, Ming-Wei Lai, Ruey-Fen Liou
Phytophthora parasitica is a notorious oomycete pathogen that causes severe disease in a wide variety of crop species. Infection of plants involves mainly its asexual life stage, including papillate sporangia and biflagellated zoospores, which are the primary dispersal and infection agents of this pathogen. Calcium signaling has been thought as the key regulator for sporangium formation and zoospore differentiation. However, not much is known about the molecular players involved in these processes. In Saccharomyces cerevisiae, mating pheromone-induced death 1 (MID1) encodes a component of a putative calcium channel...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28465578/mechanistic-insights-into-manganese-oxidation-of-a-soil-borne-mn-ii-oxidizing-escherichia-coli-strain-by-global-proteomic-and-genetic-analyses
#11
Zhiyong Wang, Jieping Wang, Jin Liu, Hong Chen, Mingshun Li, Lin Li
An iTRAQ-based comparative and quantitative proteomics analysis of a soil-borne Mn(II)-oxidizing bacterium, Escherichia coli MB266, was conducted during the exponential and stationary growth phases. A total of 1850 proteins were identified in 4 samples, of which 373 and 456 proteins were significantly up- or down-regulated in at least one pairwise comparison, respectively. The iTRAQ data indicated that several enzymes involved in fatty acid metabolism (i.e., FabA, FabD and FabZ) and pyruvate metabolism (particularly pyruvate oxidase PoxB) were significantly up-regulated, while those related to the tricarboxylic acid cycle (such as FrdB, FumB and AcnA) and methylcitrate cycle (i...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28460960/influence-of-processing-parameters-and-stress-adaptation-on-the-inactivation-of-listeria-monocytogenes-by-non-thermal-atmospheric-plasma-ntap
#12
Tamara Calvo, Avelino Álvarez-Ordóñez, Miguel Prieto, Montserrat González-Raurich, Mercedes López
This study evaluated the effectiveness of Non-Thermal Atmospheric Plasma (NTAP) treatments against Listeria. Firstly, the impact of gas composition and flow rate on L. monocytogenes and L. innocua (used as a surrogate) inactivation by NTAP was monitored. Secondly, the influence of stress adaptation (growth under suboptimal conditions, using a wide range of temperatures and media acidified up to pH5.5 with citric, lactic, malic or hydrochloric acid, or short-term exposure to acid, cold or thermal shocks) on L...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28457391/cross-talk-between-sirtuin-1-and-high-mobility-box-1-in-steatotic-liver-graft-preservation
#13
M A Zaouali, A Panisello, A Lopez, E Folch, C Castro-Benítez, R Adam, J Roselló-Catafau
BACKGROUND: Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide +-dependent histone deacetylase that regulates various pathways involved in ischemia-reperfusion injury (IRI). Moreover, high-mobility group box 1 protein (HMGB1) has also been involved in inflammatory processes during IRI. However, the roles of both SIRT1 and HMGB1 in liver preservation is poorly understood. In this communication, we evaluated the potential relationship between SIRT1 and HMGB1 in steatotic and non-steatotic liver grafts preserved in Institute Georges Lopez solution (IGL-1) preservation solution enriched or not enriched with trimetazidine (TMZ)...
May 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28457014/unusual-dimerization-of-a-bccsp-mutant-leads-to-reduced-conformational-dynamics
#14
Alonso I Carvajal, Gabriel Vallejos, Elizabeth A Komives, Víctor Castro-Fernández, Diego A Leonardo, Richard C Garratt, César A Ramírez-Sarmiento, Jorge Babul
Cold shock proteins (Csp) constitute a family of ubiquitous small proteins that act as RNA-chaperones to avoid cold-induced termination of translation. All members contain two subdomains composed of 2 and 3 β-strands, respectively, which are connected by a hinge loop and fold into a β-barrel. Bacillus caldolyticus Csp (BcCsp) is one of the most studied members of the family in terms of its folding, function, and structure. This protein has been described as a monomer in solution, although a recent crystal structure showed dimerization via domain swapping (DS)...
April 29, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28455764/heterologous-expression-of-three-camellia-sinensis-small-heat-shock-protein-genes-confers-temperature-stress-tolerance-in-yeast-and-arabidopsis-thaliana
#15
Mingle Wang, Zhongwei Zou, Qinghui Li, Huahong Xin, Xujun Zhu, Xuan Chen, Xinghui Li
CsHSP17.7, CsHSP18.1, and CsHSP21.8 expressions are induced by heat and cold stresses, and CsHSP overexpression confers tolerance to heat and cold stresses in transgenic Pichia pastoris and Arabidopsis thaliana. Small heat shock proteins (sHSPs) are crucial for protecting plants against biotic and abiotic stresses, especially heat stress. However, knowledge concerning the functions of Camellia sinensis sHSP in heat and cold stresses remains poorly understood. In this study, three C. sinensis sHSP genes (i.e...
April 28, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28442397/molecular-cloning-characterization-and-expression-analysis-of-heat-shock-protein-90-in-albino-northern-snakehead-channa-argus
#16
Aiguo Zhou, Shaolin Xie, Zhenlu Wang, Muhammad Junaid, Lanfen Fan, Chao Wang, Qiao Ye, Yanfeng Chen, De-Sheng Pei, Jixing Zou
The great albino northern snakehead Channa argus is habitual to only the Sichuan Jialing Rivers System in China, making its introduction difficult to other riverine systems. Here, we characterized heat shock protein 90 (AcaHSP90) and probed its molecular responses toward the environmental stressors that C. argus can face during its introduction and breeding in the other southern latitudes of China. To serve the purpose, cDNA encoding of AcaHSP90 were cloned and characterized in albino C. argus. The cDNA was 2752bps that contained an open reading frame (ORF), encoding a 726-amino-acid polypeptide of 83...
April 22, 2017: Gene
https://www.readbyqxmd.com/read/28435055/cloning-and-characterization-of-a-new-cold-adapted-and-thermo-tolerant-%C3%AE-carrageenase-from-marine-bacterium-flavobacterium-sp-ys-80-122
#17
Shangyong Li, Jianhua Hao, Mi Sun
ι-Carrageenases play a role in marine ι-carrageenan degradation, and their enzymatic hydrolysates are thought to be excellent antioxidants. In this study, we identified a new ι-carrageenase, encoded by cgiF, in psychrophilic bacterium Flavobacterium sp. YS-80-122. The deduced ι-carrageenase, CgiF, belongs to glycoside hydrolase family 82 and shows less than 40% amino acid identity with characterized ι-carrageenases. The activity of recombinant CgiF peaked at 30°C (1,207.8U/mg). Notably, CgiF is a cold-adapted ι-carrageenase, which showed 36...
April 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28434688/stability-of-heat-stable-live-attenuated-rotavirus-vaccine-rotasiil%C3%A2
#18
Sameer P Naik, Jagdish K Zade, Rajendra N Sabale, Sambhaji S Pisal, Ravi Menon, Subhash G Bankar, Sunil Gairola, Rajeev M Dhere
Vaccines currently available across the globe are stored and transported in a continuous cold-chain at 2-8°C or below -20°C. A temperature excursion outside this range affects the potency of the vaccines. Such vaccines need to be discarded leading wastage. The Rotavirus disease burden is predominantly reported in developing and low-income countries and therefore, has entered or poised to enter their national immunization programs. These countries already have several limitations for effective storage, maintenance and distribution of vaccines in a cold-chain and this introduction is expected to further stress this fragile ecosystem...
May 19, 2017: Vaccine
https://www.readbyqxmd.com/read/28433751/elevated-chaperone-proteins-are-a-feature-of-winter-freeze-avoidance-by-larvae-of-the-goldenrod-gall-moth-epiblema-scudderiana
#19
Guijun Zhang, Janet M Storey, Kenneth B Storey
Winter survival for many insect species includes a need to maintain metabolic homeostasis and structural/functional integrity of macromolecules not only over a wide range of cold temperatures but also in response to rapid temperature change. Chaperones are well-known to protect/stabilize protein structure with regard to heat stress but less is known about their potential involvement in long-term protection of the proteome at subzero temperatures. The present study assessed the participation of chaperone proteins in the cold hardiness of larvae of the goldenrod gall moth, Epiblema scudderiana (Clemens) (Lepidoptera, Olethreutidae), monitoring changes in nine proteins over the winter months as well as their responses to laboratory cold acclimation or anoxia exposure...
April 19, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28422612/assessing-non-suicidal-self-injury-in-the-laboratory
#20
Brooke Ammerman, Mitchell E Berman, Michael S McCloskey
OBJECTIVES: The majority of non-suicidal self-injury (NSSI) research has used self- or clinician-rated measures of behavior which (a) are subject to reporting biases, or (b) have limited use in experimental designs which could illuminate causal relationships. Laboratory-based behavioral tasks have therefore been developed to assess NSSI-related behaviors more directly. METHODS: We reviewed the behavioral methods that have been developed to assess NSSI tendencies or behaviors over the past thirty-years...
April 19, 2017: Archives of Suicide Research: Official Journal of the International Academy for Suicide Research
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