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https://www.readbyqxmd.com/read/28194325/serum-yb-1-y-box-binding-protein-1-as-a-biomarker-of-bone-disease-progression-in-patients-with-breast-cancer-and-bone-metastases
#1
A R Ferreira, M Bettencourt, I Alho, A L Costa, A R Sousa, A Mansinho, C Abreu, C Pulido, D Macedo, I Vendrell, T R Pacheco, L Costa, S Casimiro
YB-1 (Y-box binding protein 1) is a multifunctional cold-shock protein that has been implicated in all hallmarks of cancer. Elevated YB-1 protein level was associated with poor prognosis in several types of cancers, including breast cancer (BC), where it is a marker of decreased overall survival (OS) and distant metastasis-free survival across all subtypes. YB-1 is also secreted by different cell types and may act as an extracellular mitogen; however the pathological implications of the secreted form of YB-1 (sYB-1) are unknown...
March 2017: Journal of Bone Oncology
https://www.readbyqxmd.com/read/28176661/cold-shock-protein-a-from-corynebacterium-pseudotuberculosis-role-of-electrostatic-forces-in-the-stability-of-the-secondary-structure
#2
Monika A Coronado, Icaro P Caruso, Vinícius M De Oliveira, Vinícius G Contessoto, Vitor B P Leite, Raghuvir K Arni, Raphael J Eberle
The conformational stability of the Cold shock protein A (CspA) from C. pseudotuberculosis (Cp), a nucleic acid binding protein in function of pH and salt concentration was examined by using differential scanning calorimetry and CD spectroscopy in combination with computational analysis to identify the specify amino acids undergoing change. Our approach identified a sodium-binding site in CpCspA and at pH 8.0 a significant reduction in the β-sheet content was observed which resulted in a decrease of the protein thermal stability...
7, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28166240/involvement-of-multiple-stressors-induced-by-non-thermal-plasma-charged-aerosols-during-inactivation-of-airborne-bacteria
#3
Nachiket D Vaze, Sin Park, Ari D Brooks, Alexander Fridman, Suresh G Joshi
A lab-scale, tunable, single-filament, point-to-point nonthermal dieletric-barrier discharge (DBD) plasma device was built to study the mechanisms of inactivation of aerosolized bacterial pathogens. The system inactivates airborne antibiotic-resistant pathogens efficiently. Nebulization mediated pre-optimized (4 log and 7 log) bacterial loads were challenged to plasma-charged aerosols, and lethal and sublethal doses determined using colony assay, and cell viability assay; and the loss of membrane potential and cellular respiration were determined using cell membrane potential assay and XTT assay...
2017: PloS One
https://www.readbyqxmd.com/read/28155127/attenuation-of-exercise-induced-heat-shock-protein-72-expression-blunts-improvements-in-whole-body-insulin-resistance-in-rats-with-type-2-diabetes
#4
Takamasa Tsuzuki, Hiroyuki Kobayashi, Toshinori Yoshihara, Ryo Kakigi, Noriko Ichinoseki-Sekine, Hisashi Naito
Heat shock proteins (HSPs) play an important role in insulin resistance and improve the cellular stress response via HSP induction by exercise to treat type 2 diabetes. In this study, the effects of exercise-induced HSP72 expression levels on whole-body insulin resistance in type 2 diabetic rats were investigated. Male 25-week-old Otsuka Long-Evans Tokushima Fatty rats were divided into three groups: sedentary (Sed), trained in a thermal-neutral environment (NTr: 25 °C), and trained in a cold environment (CTr: 4 °C)...
February 2, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28144636/comparative-study-of-growth-and-gonad-maturation-in-diploid-and-triploid-marine-medaka-oryzias-dancena
#5
In-Seok Park, Hyun Woo Gil, Tae Ho Lee, Yoon Kwon Nam, Dong Soo Kim
The marine medaka, Oryzias dancena is a suitable sample as a laboratory animal because it has a small size and clearly distinguishes between female and male. Data on the growth and maturity of the diploid and triploid sea cucurbit species suitable for laboratory animals are very useful for studying other species. Triploidy was induced in the marine medaka by cold shock treatment (0°C) of fertilized eggs for 45 min, applied two minutes after fertilization. The diploid and triploid male fish were larger than their female counterparts (P<0...
December 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28138902/ectopic-expression-of-plant-rna-chaperone-offering-multiple-stress-tolerance-in-e-coli
#6
Bushra Jabeen, S M Saqlan Naqvi, Tariq Mahmood, Tasawar Sultana, Madiha Arif, Fariha Khan
Members of the plant glycine-rich RNA-binding proteins (GR-RBPs) family have been reported in flowering, development, circadian rhythms, biotic and abiotic stresses. Particularly, GR-RBPs are reported to function as RNA chaperones, promoting growth and acclimation during cold shock. It is indispensable to further question the efficacy and mechanism of GR-RBPs under various environmental strains. Monitoring the expression of stress-regulated proteins under stress conditions has been a beneficial strategy to study their functional roles...
January 30, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28128346/a-lin28-homologue-reprograms-differentiated-cells-to-stem-cells-in-the-moss-physcomitrella-patens
#7
Chen Li, Yusuke Sako, Akihiro Imai, Tomoaki Nishiyama, Kari Thompson, Minoru Kubo, Yuji Hiwatashi, Yukiko Kabeya, Dale Karlson, Shu-Hsing Wu, Masaki Ishikawa, Takashi Murata, Philip N Benfey, Yoshikatsu Sato, Yosuke Tamada, Mitsuyasu Hasebe
Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3'-untranslated region (3'-UTR)...
January 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28126922/rna-binding-and-chaperone-activity-of-the-e-coli-cold-shock-protein-cspa
#8
Enrico Rennella, Tomáš Sára, Michael Juen, Christoph Wunderlich, Lionel Imbert, Zsofia Solyom, Adrien Favier, Isabel Ayala, Katharina Weinhäupl, Paul Schanda, Robert Konrat, Christoph Kreutz, Bernhard Brutscher
Ensuring the correct folding of RNA molecules in the cell is of major importance for a large variety of biological functions. Therefore, chaperone proteins that assist RNA in adopting their functionally active states are abundant in all living organisms. An important feature of RNA chaperone proteins is that they do not require an external energy source to perform their activity, and that they interact transiently and non-specifically with their RNA targets. So far, little is known about the mechanistic details of the RNA chaperone activity of these proteins...
January 26, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28116450/cold-agglutinin-disease-an-unusual-cause-of-shock-in-the-icu
#9
Hasan M Al-Dorzi, Hind Salama, Areej Almugairi, Yaseen M Arabi
No abstract text is available yet for this article.
January 23, 2017: Intensive Care Medicine
https://www.readbyqxmd.com/read/28115385/freshwater-cyanobacterial-interactions-with-bacterial-antagonists
#10
Omneya Ahmed Osman, Sara Beier, Manfred Grabherr, Stefan Bertilsson
: Cyanobacterial and algal mass development or "blooms" have severe effects on freshwater and marine systems around the world. Many of these phototrophs produce a variety of potent toxins, contribute to oxygen depletion, and affect water quality in several ways. Coexisting antagonists such as cyanolytic bacteria, hold the potential to suppress, or even terminate such blooms, yet the nature of this interaction is not well studied. We isolated 31 cyanolytic bacteria affiliated with the genera Pseudomonas, Stenotrophomonas, Acinetobacter and Delftia from three eutrophic freshwater lakes in Sweden, and selected four phylogenetically diverse bacterial strains with strong to moderate lytic activity...
January 23, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28115289/profibrotic-ihg-1-complexes-with-renal-disease-associated-hspa5-and-trap1-in-mitochondria
#11
Una Bhreathnach, Brenda Griffin, Eoin Brennan, Leah Ewart, Debra Higgins, Madeline Murphy
The highly conserved mitochondrial protein induced in high glucose-1 (IHG-1) functions to maintain mitochondrial quality and is associated with the development of fibrosis in diabetic nephropathy. Towards identifying novel approaches to treating diabetic kidney disease, IHG-1-protein-protein interactions were investigated using epitope-tagged immunoprecipitation analyses followed by mass spectrometry. Here we show that IHG-1 is solely expressed in mitochondria and localised to the inner mitochondrial membrane, the region where mitochondrial reactive oxygen species are generated...
January 20, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28108740/positive-expression-of-y-box-binding-protein-1-and-prognosis-in-non-small-cell-lung-cancer-a-meta-analysis
#12
Liang Jiang, Gao-Le Yuan, Qi-Lian Liang, Hui-Jie Zhang, Jie Huang, Shao-Ang Cheng, Xiao-Xia Peng
BACKGROUND: Y-box binding protein 1 (YB-1) belongs to the cold shock domain protein family involved in transcription and translation. We conducted a meta-analysis of the association between YB-1 expression and the survival and clinicopathological features in NSCLC. METHODS: PubMed and Embase were searched to identify studies that evaluated the YB-1 expression (by immunohistochemistry) and overall survival (OS) in NSCLC. Hazard ratios (HRs) and 95% confidence intervals (CI) of OS were pooled...
January 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28102369/corrigendum-purification-and-characterization-of-a-novel-cold-shock-protein-like-bacteriocin-synthesized-by-bacillus-thuringiensis
#13
Tianpei Huang, Xiaojuan Zhang, Jieru Pan, Xiaoyu Su, Xin Jin, Xiong Guan
No abstract text is available yet for this article.
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28089856/erythrocyte-heat-shock-protein-responses-to-chronic-in-vivo-and-acute-in-vitro-temperature-challenge-in-diploid-and-triploid-salmonids
#14
Pillai V Saranyan, Neil W Ross, Tillmann J Benfey
This research investigated how ploidy level (diploid versus triploid) affects the heat shock protein (HSP) response in erythrocytes under different thermal stress regimes, both in vivo and in vitro, in Atlantic salmon (Salmo salar) and brook charr (Salvelinus fontinalis) in order to address the question of why triploids typically have reduced thermal tolerance. A preliminary study confirmed that identical volumes of diploid and triploid erythrocytes (which equates to a smaller number of larger cells for triploids compared to diploids) did not differ in total protein synthesis rates...
January 13, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28072431/expression-patterns-of-three-genes-under-short-and-long-term-cold-exposure-in-thitarodes-pui-lepidoptera-hepialidae-a-host-of-ophiocordyceps-sinensis
#15
Q Min, S Y Cheng, J F Xi, J Ma, T R Xin, B Xia, Z W Zou
:   BACKGROUND: Thitarodes larvae are the host of the caterpillar fungus Ophiocordyceps sinensis. Low temperature is the main environmental limitation for larvae growth. OBJECTIVE: To better understand the cold adaption process in T. pui larvae, the expression patterns of trehalose-6-phosphate synthase (TpTPS), heat shock protein 70 (TpHSP70), and heat shock protein 90 (TpHSP90) were investigated upon short and long-term exposure to 0°C. MATERIALS AND METHODS: The 6th instar T...
November 2016: Cryo Letters
https://www.readbyqxmd.com/read/28068641/oil-palm-drought-inducible-dreb1-induced-expression-of-dre-crt-and-non-dre-crt-containing-genes-in-lowland-transgenic-tomato-under-cold-and-peg-treatments
#16
Azzreena Mohamad Azzeme, Siti Nor Akmar Abdullah, Maheran Abd Aziz, Puteri Edaroyati Megat Wahab
Dehydration-responsive element binding (DREB) transcription factor plays an important role in controlling the expression of abiotic stress responsive genes. An intronless oil palm EgDREB1 was isolated and confirmed to be a nuclear localized protein. Electrophoretic mobility shift and yeast one-hybrid assays validated its ability to interact with DRE/CRT motif. Its close evolutionary relation to the dicot NtDREB2 suggests a universal regulatory role. In order to determine its involvement in abiotic stress response, functional characterization was performed in oil palm seedlings subjected to different levels of drought severity and in EgDREB1 transgenic tomato seedlings treated by abiotic stresses...
December 27, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28056497/taxonomic-distribution-repeats-and-functions-of-the-s1-domain-containing-proteins-as-members-of-the-ob-fold-family
#17
Evgeniia I Deryusheva, Andrey V Machulin, Olga M Selivanova, Oxana V Galzitskaya
Proteins of the nucleic acid-binding proteins superfamily perform such functions as of processing, transport, storage, stretching, translation and degradation of RNA. It is one of the 16 superfamilies containing the OB-fold in protein structures. Here, we have analyzed the superfamily of nucleic acid-binding proteins (the number of sequences exceeds 200000) and obtained that this superfamily prevalently consists of proteins containing the cold shock DNA-binding domain (about 131000 protein sequences). Proteins containing the S1 domain compose 57% from the cold shock DNA-binding domain family...
January 5, 2017: Proteins
https://www.readbyqxmd.com/read/28040037/acoustics-from-a-rectangular-supersonic-nozzle-exhausting-over-a-flat-surface
#18
Pablo Mora, Florian Baier, K Kailasanath, Ephraim J Gutmark
A jet exhausting over a plate at different distances away from the nozzle was investigated to simulate jets exhausting over airframe surfaces and the jet-ground interaction during take-off and landing operations. A supersonic rectangular nozzle of 2:1 aspect ratio and 1.5 design Mach number was tested with and without the plate. Far-field acoustics from the cold and heated jets at over-expanded, design, and under-expanded conditions were measured at the reflected, sideline, and shielded azimuthal directions...
December 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/28026125/effects-of-temperature-on-fitness-costs-in-chlorpyrifos-resistant-brown-planthopper-nilaparvata-lugens-st%C3%A3-l-hemiptera-delphacidae
#19
Baojun Yang, Meiling Liu, Yixi Zhang, Zewen Liu
Insecticide resistance is inevitable if an insecticide is widely used to control insect pests. Fortunately, the resistance-associated fitness costs often give chances to manage resistances. In most cases, the fitness cost in resistant insects is often evaluated under the laboratory condition for insect development, which limits its practical application in pest control in fields. In a laboratory population R9 with 253-fold resistance to chlorpyrifos after 9-generation selection with chlorpyrifos, the relative fitness was only 0...
December 27, 2016: Insect Science
https://www.readbyqxmd.com/read/28024193/higher-production-of-c-phycocyanin-by-nitrogen-free-diazotrophic-cultivation-of-nostoc-sp-nk-and-simplified-extraction-by-dark-cold-shock
#20
Na Kyeong Lee, Hee-Mock Oh, Hee-Sik Kim, Chi-Yong Ahn
Nostoc sp. NK (KCTC 12772BP) was isolated and cultivated in a BG11 medium and a nitrate-free BG11 medium (BG110). To enhance C-phycocyanin (C-PC) content in the cells, different fluorescent lamps (white, plant, and red) were used as light sources for complementary chromatic adaptation (CCA). The maximum biomass productivity was 0.42g/L/d and 0.32g/L/d under BG11 and BG110 conditions, respectively. The maximum C-PC contents were 8.4% (w/w) under white lamps, 13.6% (w/w) under plant lamps, and 18% (w/w) under BG110 and the red light condition...
March 2017: Bioresource Technology
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