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bronchial asthma and IL 35

Fatemeh Fattahi, Corry-Anke Brandsma, Monique Lodewijk, Marjan Reinders-Luinge, Dirkje S Postma, Wim Timens, Machteld N Hylkema, Nick H T Ten Hacken
BACKGROUND: Interleukin (IL)-17 plays a critical role in numerous immune and inflammatory responses and was recently suggested to contribute to the pathogenesis of nonatopic (non-eosinophil/neutrophil-dominant) asthma. We aimed to compare expression of IL-17 in bronchial airways between atopic and nonatopic asthmatics, with/without inhaled corticosteroid (ICS) use and to identify its major cellular source. METHODS: Bronchial biopsies from 114 patients with mild-to-moderate asthma were investigated: 33 nonatopic, 63 non-corticosteroid users, 90 nonsmokers...
2016: PloS One
J H Zhang, G H Zhou, T T Wei, Z S Chang
We investigated the association between the interleukin 4 gene (IL-4) -590C>T polymorphism and forced expiratory volume in one second (FEV1) values, immunoglobulin E (IgE) levels, and susceptibility to asthma in Uighur children. IL-4 -590C>T frequencies were analyzed in 38 bronchial asthmatic patients and 35 non-asthmatic controls. Polymerase chain reaction and direct sequencing were applied to determine the residue at position -590 of IL-4. Total serum IgE levels were detected by enzyme-linked immunosorbent assay, while lung function was examined by professionals...
2016: Genetics and Molecular Research: GMR
Maartje A Nieuwenhuis, Matteusz Siedlinski, Maarten van den Berge, Raquel Granell, Xingnan Li, Marijke Niens, Pieter van der Vlies, Janine Altmüller, Peter Nürnberg, Marjan Kerkhof, Onno C van Schayck, Ronald A Riemersma, Thys van der Molen, Jan G de Monchy, Yohan Bossé, Andrew Sandford, Carla A Bruijnzeel-Koomen, Roy G van Wijk, Nick H Ten Hacken, Wim Timens, H Marike Boezen, John Henderson, Michael Kabesch, Judith M Vonk, Dirkje S Postma, Gerard H Koppelman
BACKGROUND: Genome wide association studies (GWAS) of asthma have identified single nucleotide polymorphisms (SNPs) that modestly increase the risk for asthma. This could be due to phenotypic heterogeneity of asthma. Bronchial hyperresponsiveness (BHR) is a phenotypic hallmark of asthma. We aim to identify susceptibility genes for asthma combined with BHR and analyse the presence of cis-eQTLs among replicated SNPs. Secondly, we compare the genetic association of SNPs previously associated with (doctor diagnosed) asthma to our GWAS of asthma with BHR...
July 20, 2016: Allergy
X Huo, K Zhang, L Yi, Y Mo, Y Liang, J Zhao, Z Zhang, Y Xu, G Zhen
BACKGROUND: Airway eosinophilic inflammation is a pivotal feature of asthma. Epithelial cells play critical roles in airway eosinophilia. We hypothesized that epithelial microRNAs (miRNAs) are involved in airway eosinophilia. OBJECTIVE: This study investigated the associations between epithelial and plasma miR-181b-5p and airway eosinophilic inflammation, and the possible mechanism by which miR-181b-5p participates in eosinophilic inflammation. METHODS: Epithelial miRNAs expression was profiled by miRNA array in eight subjects with asthma and four healthy controls...
October 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Yan Li, Xiuhe Pan, Xiao Peng, Shubo Li, Yanchun Zhou, Xiaoxuan Zheng, Mingcai Li
OBJECTIVE AND DESIGN: Asthma is thought to result from the generation of T helper type 2 (Th2) responses, leading to bronchial inflammation. Interleukin (IL)-35 is a recently described member of IL-12 cytokine family that plays a critical role in influencing Th cell differentiation and inflammatory processes. The aim of this study was to examine the effect of adenovirus expressing IL-35 (AdIL-35) on allergic airway hyperresponsiveness (AHR) and inflammation in a mouse model of asthma...
October 2015: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Lan Wang, Qiang Chen, Cuiqin Shi, Hanjing Lv, Xianghuai Xu, Li Yu
OBJECTIVE: This study aimed to investigate the changes of serum levels of TNF-α, IL-5 and IgE in mycoplasma pneumonia (MP) infection patients with or without bronchial asthma and explore its clinical importance. METHODS: ELISA and Western blot assay were performed to detect the serum levels of TNF-α, IL-5 and IgE in 35 healthy subjects, 45 patients with MP infection and 40 bronchial asthma patients with MP infection. RESULTS: The serum levels of TNF-α, IL-5 and IgE in MP infection patients and asthma patients with MP infection were significantly higher than those in healthy subjects (P<0...
2015: International Journal of Clinical and Experimental Medicine
Yu-xin Jiang, Kang Yin, Wen-jie Jin, Lu-yi Wu, Chao-pin Li
OBJECTIVE: To investigate the effect of Der f 1 mRNA molecules for specific immunotherapy on murine model of asthma. METHODS: Fifty BALB/c mice were randomly divided into 5 groups: PBS group, Der f 1 sensitization group, Der f 1 specific immunotherapy (SIT) group, beta-actin mRNA SIT group, and Derf 1 mRNA SIT group. On days 0, 7 and 14, mice in PBS group received PBS injection; mice in the other groups were intraperitoneally injected with 10 microg Derf 1. At day 21, the mice in the 4 experimental groups were challenged with a 30-min inhaled dose of Der f 1 (100 microg/ml) for 7 successive days...
August 2014: chinese Journal of Parasitology & Parasitic Diseases
Ronen Bar-Yoseph, Lea Bentur, Aviv Goldbart, Galit Livnat, Fahed Hakim, Yosef Weisman, Dov Tiosano
CONTEXT: Previous studies have reported an association between vitamin D deficiency and asthma. Hereditary 1,25-dihydroxyvitamin D-resistant rickets (HVDRR) patients provide a natural model to assess the role of the vitamin D receptor (VDR) in regulating human lung immune responses and airway hyperreactivity. OBJECTIVES: The aim of the study was to determine the role of the VDR on lung functions, airways, and systemic markers of inflammation and allergy in HVDRR patients...
September 2014: Journal of Clinical Endocrinology and Metabolism
Yu Utsumi, Nobuhito Sasaki, Hiromi Nagashima, Naomi Suzuki, Yutaka Nakamura, Masahiro Yamashita, Hitoshi Kobayashi, Kohei Yamauchi
BACKGROUND: A single nucleotide polymorphism (SNP; rs20541) in the IL-13 gene has been recognized as a risk factor for asthma. This SNP causes Arg to Gln (Q) substitution at position 110 in the mature IL-13 protein. We have recently showed that FEV1 in asthmatics with the Q110 variant of IL-13 declined faster, and progressive airway remodeling was observed in these subjects (Wynn, 2003 [1]). However, the effects of the IL-13 variant on airway hyperresponsiveness (AHR) remain to be elucidated...
September 2013: Respiratory Investigation
Elham M Hossny, Shereen S El-Sayed, Eman S El-Hadidi, Sherif R Moussa
BACKGROUND: : Interleukin 18 (IL-18) is reported to have regulatory functions on Th1 and Th2 cytokine production and proinflammatory effects through promoting recruitment of memory Th1 cells to inflammatory sites. We sought to investigate the expression of the serum IL-18 in childhood bronchial asthma in relation to disease activity and severity. METHODS: : Serum IL-18 was measured by enzymatic immunoassay in 25 asthmatic children during exacerbation and after complete quiescence of symptoms and signs...
May 2009: World Allergy Organization Journal
Marc A Riedl, David Diaz-Sanchez, William S Linn, Henry Gong, Kenneth W Clark, Richard M Effros, J Wayne Miller, David R Cocker, Kiros T Berhane
To improve understanding of human health risks from exposure to diesel exhaust particles (DEP*), we tested whether immunologic effects previously observed in the human nose also occur in the lower airways. Our overall hypothesis was that cell influx and production of cytokines, chemokines, immunoglobulin E (IgE), and other mediators, which would be measurable in sputum and blood, occur in people with asthma after realistic controlled exposures to diesel exhaust (DE). In Phase 1 we tested for direct effects of DE in subjects with clinically undifferentiated mild asthma...
February 2012: Research Report (Res Rep Health Eff Inst)
E Tseliou, P Bakakos, K Kostikas, G Hillas, K Mantzouranis, P Emmanouil, D Simoes, M Alchanatis, S Papiris, S Loukides
BACKGROUND: Airway and vascular remodeling may play a prominent role in the clinical severity of severe refractory asthma (SRA). Angiopoietin-1 (Ang-1) is an essential mediator of angiogenesis by establishing vascular integrity, whereas angiopoietin-2 (Ang-2) acts as its natural inhibitor. OBJECTIVE: We aimed to determine the levels of angiopoietins in sputum supernatants of patients with SRA and to investigate the possible associations with mediators and cells involved in both the inflammatory and the vascular remodeling processes...
March 2012: Allergy
You-Lan Yang, Hsin-Te Hsu, Kuo-Hsien Wang, Cheng-Ying Han, Chien-Ming Chen, Chi-Ming Chen, Wun-Chang Ko
BACKGROUND: Hesperetin was reported to selectively inhibit phosphodiesterase 4 (PDE4). While hesperetin-7,3'-O-dimethylether (HDME) is a synthetic liposoluble hesperetin. Therefore, we were interested in investigating its selectivity on PDE4 and binding ability on high-affinity rolipram-binding sites (HARBs) in vitro, and its effects on ovalbumin-induced airway hyperresponsiveness in vivo, and clarifying its potential for treating asthma and chronic obstructive pulmonary disease (COPD)...
2011: Journal of Biomedical Science
J Yamashita, T Shirakusa, N Fujino, T Kiyama, E Kinuwaki, M Ogawa
It has been suggested that a proportion of patients with cancer have an ongoing acute phase response indicated by a raised C-reactive protein (CRP). To examine whether an acute phase protein response is associated with circulating interleukin-6 (IL-6) concentrations in patients with lung cancer, we measured serum levels of CRP and interleukin (IL)-6 in 176 patients with lung cancer and 48 patients with other pulmonary diseases (28 diffuse pulmonary infiltrates, 15 benign lung tumors, and 5 bronchial asthmas)...
March 1995: Oncology Reports
Elisabeth Fabian, Peter Pölöskey, Lajos Kósa, Ibrahim Elmadfa, Lajos Attila Réthy
BACKGROUND: This study aimed to investigate the relationship between antioxidant enzyme activities, extent of airway inflammation, and systemic oxidative stress in children suffering from atopic asthma. METHODS: A total of 35 asthmatic (AG) and 21 healthy children (CG) participated in this study. The volume of fractionated exhaled NO (Fe(no)) was measured with the NIOX test system. The activities of the erythrocyte antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), and the total antioxidant capacity (TAC) were determined photometrically...
May 2011: Journal of Asthma: Official Journal of the Association for the Care of Asthma
K Mutalithas, C Guillen, C Raport, R Kolbeck, D Soler, C E Brightling, I D Pavord, A J Wardlaw
BACKGROUND: Chemokines and their receptors could play key roles in the recruitment of T cells to the asthmatic lung. CCR8 is preferentially expressed on T-helper type 2 cells, and is thought to play a role in the pathogenesis of human asthma. OBJECTIVE: Determine the expression of CCR8 on T cells in blood, bronchoalveolar lavage (BAL) and bronchial mucosa from asthmatics and normal subjects. METHODS: CCR8 expression in blood and BAL from asthma and normal subjects was studied using flow cytometry...
August 2010: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Feng-Qin Wu, Shu-Yang Zhu, Cai-Xia He, Ming-Xiao Gu
OBJECTIVE: To explore the role of Rho kinase-1 (ROCK-1) in airway inflammation of asthma by observing the effects of fasudil, a specific inhibitor of ROCK-1, on the expression of Rho kinase-1 and airway inflammation in a mouse model of asthma. METHODS: Twenty-four female BALB/c mice were randomly divided into 3 groups (n = 8 each): a control group, an asthmatic group and a treatment group. Mice in the asthmatic and the treatment groups were sensitized by intraperitoneal injection of OVA (25 microg) precipitated with 1 mg of alum in 200 microl of saline on days 1 and 15, and subsequently challenged by nebulization of 2% OVA on days 22-26...
November 2009: Chinese Journal of Tuberculosis and Respiratory Diseases
Rosalia Gagliardo, Stefania La Grutta, Pascal Chanez, Mirella Profita, Alessandra Paternò, Fabio Cibella, Jean Bousquet, Giovanni Viegi, Mark Gjomarkaj
To evaluate the relationship between pro-inflammatory and pro-remodeling mediators and severity and control of asthma in children, the levels of IL-8, MMP-9, TIMP-1 in induced sputum supernatants, the number of sputum eosinophils, as well as FeNO, were investigated in 35 asthmatic children, 12 with intermittent (IA) and 23 with moderate asthma (MA), and 9 controls (C). The patients with asthma were followed for 1 yr and sputum was obtained twice during the follow-up. Biomarker levels were correlated with the number of exacerbations...
December 2009: Pediatric Allergy and Immunology
Hae-Seong Nam, Sook Young Lee, Seung Jun Kim, Ju Sang Kim, Soon Seog Kwon, Young Kyoon Kim, Kwan Hyung Kim, Hwa Sik Moon, Jeong Sup Song, Sung Hak Park, Seok Chan Kim
PURPOSE: Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine that has been implicated in many aspects of the airway pathology in asthma. TNF-alpha blocking strategies are now being tried in asthma patients. This study investigated whether TNF-alpha blocking therapy inhibits airway inflammation and airway hyperresponsiveness (AHR) in a mouse model of asthma. We also evaluated the effect of TNF-alpha blocking therapy on cytokine production and adhesion molecule expression...
August 31, 2009: Yonsei Medical Journal
Dominique M A Bullens, A Decraene, E Dilissen, I Meyts, K De Boeck, L J Dupont, J L Ceuppens
BACKGROUND: The increased susceptibility of asthmatics to rhinovirus infection has recently been related to deficient IFN-lambda 1 (IL-29) and IFN-lambda 2/3 (IL-28) production by bronchial epithelial cells and macrophages. OBJECTIVES: Here, we studied IFN-lambda mRNA expression in the airways of stable asthmatics in comparison with healthy subjects and in relation to asthma symptoms, non-invasive parameters of airway inflammation and lung function parameters. METHODS: Airway cells were obtained by sputum induction, in 14 healthy and 35 asthmatic adults and 12 asthmatic school-aged children...
September 2008: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
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