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cik cytokine induced killing

Lei Wang, Na Peng, Xiaoyan Hu, Wentao Liang, Kai Liang, Guizhu Peng
Objective To evaluate the effects of cryopreservation on the proliferation and killing activity of lymphocytes, and explore a novel protocol of preparing autologous mouse cytotoxic T lymphocytes (CTLs). Methods Mononuclear cells derived from spleen (5.0×10(6)/mL) were cryopreserved in CELLBANKER2 for 6 days and recovered in water bath at 39DegreesCelsius. The fresh lymphocytes and post-cryopreservation lymphocytes were induced by CD3 mAb (100 ng/mL) and recombinant mouse interleukin 2 (rmIL-2, 100 ng/mL) to obtain cytokine-induced killer cells (CIKs)...
November 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Melanie Bremm, Sabine Huenecke, Olga Zimmermann, Verena Pfirrmann, Andrea Quaiser, Halvard Bonig, Jan Soerensen, Thomas Klingebiel, Eva Rettinger, Peter Bader, Claudia Cappel
BACKGROUND: Cytokine-induced-killer (CIK) cells are a promising immunotherapeutic approach for impending relapse following hematopoietic stem cell transplantation (HSCT). However, there is a high risk for treatment failure associated with severe graft versus host disease (GvHD) necessitating pharmaceutical intervention post-transplant. Whether immunosuppression with mycophenolate mofetil (MMF) or Ciclosporin A (CsA) influences the cytotoxic effect of CIK cell immunotherapy is still an open issue...
2016: Journal of Translational Medicine
Paola Iudicone, Daniela Fioravanti, Elisabetta Cicchetti, Ilaria Grazia Zizzari, Annino Pandolfi, Rita Scocchera, Raffaella Fazzina, Luca Pierelli
CIK cells are a subset of effector lymphocytes endowed with a non-MHC restricted anti-tumor activity making them an appealing and promising cell population for adoptive immunotherapy. CIK are usually generated ex-vivo by initial priming with Interferon-γ (IFN-γ) and monoclonal antibody against CD3 (anti-CD3), followed by culture in medium containing Interleukin-2 (IL-2). Interleukin-15 (IL-15) shares with IL-2 similar biological functions and recently it has been reported to induce CIK with increased anti-leukemic potential...
September 8, 2016: Human Immunology
Claudia Cappel, Sabine Huenecke, Anica Suemmerer, Stephanie Erben, Eva Rettinger, Verena Pfirrmann, Annekathrin Heinze, Olga Zimmermann, Thomas Klingebiel, Evelyn Ullrich, Peter Bader, Melanie Bremm
BACKGROUND: Neuroblastoma (NB) is the most common solid extracranial tumor in childhood. Despite advances in therapy, the prognosis is poor and optimized therapies are urgently needed. Therefore, we investigated the antitumor potential of interleukin-15 (IL-15)-activated cytokine-induced killer (CIK) cells against different NB cell lines. PROCEDURE: CIK cells were generated from peripheral blood mononuclear cells by the stimulation with interferon-γ (IFN-γ), IL-2, OKT-3 and IL-15 over a period of 10-12 days...
July 19, 2016: Pediatric Blood & Cancer
Jiong-Huang Chen, Jian-Yang Xiang, Guo-Ping Ding, Li-Ping Cao
OBJECTIVE: The aim of our study is to observe the impact of cholangiocarcinoma-derived exosomes on the antitumor activities of cytokine-induced killer (CIK) cells and then demonstrate the appropriate mechanism. METHODS: Tumor-derived exosomes (TEXs), which are derived from RBE cells (human cholangiocarcinoma line), were collected by ultracentrifugation. CIK cells induced from peripheral blood were stimulated by TEXs. Fluorescence-activated cell sorting (FACS) was performed to determine the phenotypes of TEX-CIK and N-CIK (normal CIK) cells...
July 2016: Journal of Zhejiang University. Science. B
Zan-Mei Xu, Ying Lu, Lan-Jun Zhao, Jin Liu, Xian-Wen Hu, Chu-Tse Wu, Hai-Feng Duan
OBJECTIVE: To study transfection efficiency of Ad5F11p-GFP and its influence on biological characteristics of CIK and NK-92 cells in order to predict the application of Ad5F11p vector in immunotherapy. METHODS: Two kinds of immune cells, cytokine-induced killer (CIK) cells and natural-killer (NK) cell line NK-92 cells, were transfected by Ad5F11p-GFP at different multiplicity of transfection (MOI), and untransfected immune cells were used as negative control. GFP expression was determined by flow cytometry, the cell morphology was observed with microscope, the cell proliferation was analyzed by trypan blue staining, specific cytotoxicity of NK-92 cells was determined by LDH assay...
June 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yao Yang, Jingjing Zhang, Fangfang Xia, Chunlei Zhang, Qirong Qian, Xiao Zhi, Caixia Yue, Rongjin Sun, Shangli Cheng, Shan Fang, Weilin Jin, Yuming Yang, Daxiang Cui
How to realize targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy of gastric cancer has become a great challenge. Herein, we reported for the first time that human cytokine-induced killer cells (CIK) loaded with gold nanorods were used for targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy of gastric cancer. Silica-modified gold nanorods were prepared; then incubated with human cytokine-induced killer cells (CIK), resultant human CIK cells loaded with Au nanorods were evaluated for their cytotoxicity, targeted ability of gastric cancer in vitro and in vivo, immunotherapy, and photothermal therapy efficacy...
December 2016: Nanoscale Research Letters
Ri-Sheng Que, Cheng Lin, Guo-Ping Ding, Zheng-Rong Wu, Li-Ping Cao
BACKGROUND: Tumor-derived exosomes were considered to be potential candidates for tumor vaccines because they are abundant in immune-regulating proteins, whereas tumor exosomal miRNAs may induce immune tolerance, thereby having an opposite immune function. OBJECTIVE: This study was designed to separate exosomal protein and depleted exosomal microRNAs (miRNAs), increasing the immune activity of exosomes for activating dendritic cell/cytokine-induced killer cells (DC/CIKs) against pancreatic cancer (PC)...
May 2016: Journal of Zhejiang University. Science. B
Xiao-Xiang Rong, Fang Wei, Xiao-Lin Lin, Yu-Juan Qin, Lin Chen, Hui-Yan Wang, Hong-Fen Shen, Li-Ting Jia, Rao-Ying Xie, Tao-Yan Lin, Wei-Chao Hao, Jie Yang, Sheng Yang, Yu-Shuang Cheng, Wen-Hua Huang, Ai-Min Li, Yan Sun, Rong-Cheng Luo, Dong Xiao
There is an urgent need for more potent and safer approaches to eradicate cancer stem cells (CSCs) for curing cancer. In this study, we investigate cancer-killing activity (CKA) of cytokine-induced killer (CIK) cells against CSCs of hepatocellular carcinoma (HCC). To visualize CSCs in vitro by fluorescence imaging, and image and quantify CSCs in tumor xenograft-bearing mice by bioluminescence imaging, HCC cells were engineered with CSC detector vector encoding GFP and luciferase controlled by Nanog promoter...
March 2016: Oncoimmunology
Runmei Li, Fang Yan, Liang Liu, Hui Li, Baozhu Ren, Zhenzhen Hui, Xiubao Ren
Liver cancer, of which the most common form is hepatocellular carcinoma (HCC), is one of the most lethal cancers worldwide. Immunotherapy based on the direct attack of tumor cells and the stimulation of an antitumor immune response may represent a novel strategy to control HCC recurrence and metastasis. The present study reports the case of a patient with HCC, and describes the safety and feasibility of successful administration with a mass of autologous activated T cells on numerous occasions subsequent to liver transplantation (LT), in order to kill the residual tumor cells and stimulate the immune system...
March 2016: Oncology Letters
Su Li Poh, Yeh Ching Linn
We studied whether blockade of inhibitory receptors on cytokine-induced killer (CIK) cells by immune checkpoint inhibitors could increase its anti-tumour potency against haematological malignancies. CIK cultures were generated from seven normal donors and nine patients with acute myeloid leukaemia (AML), acute lymphoblastic leukaemia (ALL) or multiple myeloma (MM). The inhibitory receptors B and T lymphocyte attenuator, CD200 receptor, lymphocyte activation gene-3 (LAG-3) and T cell immunoglobulin and mucin-domain-containing-3 (TIM-3) were present at variable percentages in most CIK cultures, while cytotoxic T lymphocyte-associated protein 4 (CTLA-4), programmed death-1 (PD-1) and killer cell immunoglobulin-like receptors (KIR2DL1/2/3) were expressed at low level in most cultures...
May 2016: Cancer Immunology, Immunotherapy: CII
Xue Yin, Xin Xu, Yao Zhao, Zhan-Ju Wang, Hai-Ying Wang, Zhen-Bo Hu
OBJECTIVE: To compare several schemes of inducing and expanding the antibody-mediated high efficiency CIK (AMHE-CIK) in vitro, so as to find out a method that can acquire a large number of cells capable to kill the tumor cells in a short time. METHODS: Peripheral blood mononuclear cells (PBMNC) from healthy volunteers was isolated and activated with CD3 antibody, then were cultured with the addition of different cytokines (IL-2, IL-4, G-CSF, GM-CSF, IFN-γ, TNF-α) for 14 days in vitro...
February 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Congqi Dai, Fengjuan Lin, Ruixuan Geng, Xiaoxiao Ge, Wenbo Tang, Jinjia Chang, Zheng Wu, Xinyang Liu, Ying Lin, Zhe Zhang, Jin Li
Cytokine-induced killer (CIK) cells represent a realistic approach in cancer immunotherapy with confirmed survival benefits in the context of metastatic solid tumors. However, therapeutic effects are limited to a fraction of patients. In this study, immune-resistance elements and ideal combination therapies were explored. Initially, phenotypic analysis was performed to document CD3, CD56, NKG2D, DNAM-1, PD-L1, PD-1, CTLA-4, TIM-3, 2B4, and LAG-3 on CIK cells. Upon engagement of CIK cells with the tumor cells, expression of PD-1 on CIK cells and PD-L1 on both cells were up-regulated...
March 1, 2016: Oncotarget
Xu-Cang Wei, Di-Di Yang, Xiu-Rui Han, Yu-An Zhao, Yan-Chun Li, Li-Jie Zhang, Jiu-Ju Wang
OBJECTIVE: We investigated the effect of umbilical cord blood dendritic cells (DCs) on in vitro proliferation, immunophenotypes and levels of homologous cytokine-induced killer cells (CIK) and the toxicity on leukemia cells. METHOD: Mononuclear cell-induced DC-CIK cells derived from umbilical cord blood were collected and co-cultured in the proportion of 1:5. Cord blood CIK cells or peripheral blood DC-CIK cells were used as control. Phenotypes were analyzed by flow cytometry; vial cell counting was performed using trypan blue, and the killing activity of effector cells against leukemia cells was measured by MTT assay...
2015: International Journal of Clinical and Experimental Medicine
Fang Wei, Xiao-Xiang Rong, Rao-Ying Xie, Li-Ting Jia, Hui-Yan Wang, Yu-Juan Qin, Lin Chen, Hong-Fen Shen, Xiao-Lin Lin, Jie Yang, Sheng Yang, Wei-Chao Hao, Yan Chen, Sheng-Jun Xiao, Hui-Rong Zhou, Tao-Yan Lin, Yu-Shuang Chen, Yan Sun, Kai-Tai Yao, Dong Xiao
Cancer stem cells (CSCs) are considered to be the root cause for cancer treatment failure. Thus, there remains an urgent need for more potent and safer therapies against CSCs for curing cancer. In this study, the antitumor activity of cytokine-induced killer (CIK) cells against putative CSCs of nasopharyngeal carcinoma (NPC) was fully evaluated in vitro and in vivo. To visualize putative CSCs in vitro by fluorescence imaging, and image and quantify putative CSCs in tumor xenograft-bearing mice by in vivo bioluminescence imaging, NPC cells were engineered with CSC detector vector encoding GFP and luciferase (Luc) under control of Nanog promoter...
October 27, 2015: Oncotarget
J Z Mei, G J Liu, X J Zhang, J Z Zhao, R T Feng
We examined the effects and molecular mechanism of the epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor erlotinib on NKG2D ligand expression in human lung adenocarcinoma A549 cells and the cytotoxicity of cytokine-induced killer cells. Flow cytometry was used to detect NKG2D ligand expression in A549 cells under effects of erlotinib and EGFR downstream molecules, including LY294002 (phosphoinositide 3-kinase inhibitor), SB203580 (mitogen-activated protein kinase inhibitor), and STAT21 (signal transduction and transcription 3 inhibitor) after 24 h...
2015: Genetics and Molecular Research: GMR
Chao Li, Shujing Liang, Chunlei Zhang, Yanlei Liu, Meng Yang, Jingpu Zhang, Xiao Zhi, Fei Pan, Daxiang Cui
Dendritic cells (DCs) have displayed the promising potential in cancer immunity. How to enhance DCs immunotherapeutic effect in cancer targeted immunotherapy and prevention is still a great challenge. Herein, we report for the first time the allogenic DCs and tumor cell fused vaccine combined with cytokine induced killing cells (CIKs) for targeted imaging and enhanced immunotherapeutic efficacy of gastric cancer (GC). The fused vaccine was prepared by PEG mediated fusion between mature DCs and inactive gastric cancer MGC803 cells...
June 2015: Biomaterials
Jinying Zhang, Lingjun Zhu, Haina Du, Xiang He, Yongmei Yin, Yanhong Gu, Lianke Liu, Kaihua Lu, Renhua Guo, Ping Liu, Yongqian Shu
Cytokine-induced killer (CIK) cells have the ability to kill tumor cells in vitro and in vivo. This study aimed to evaluate the clinical effect of adjuvant immunotherapy with CIK cells on the prognosis of lung cancer patients. In the present study, we investigated the clinical outcomes of autologous CIK cell immunotherapy for patients with lung cancer in a case-control study. Our study included 60 patients who received chemotherapy combined with autologous CIK cell adoptive immunotherapy in CIK treatment group and 60 patients who received chemotherapy alone in the control group...
March 2015: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
WenJia Wang, SiHao Qin, Lei Zhao
Pretreatment with chemotherapeutic agents could sensitize human lung adenocarcinoma cells to the lyses of cytokine-induced killer (CIK) cells, however, the mechanism still unclear. We hypothesized that chemotherapeutic agents could induced immunogenic modulation (IM) and calreticulin (CRT) expression and enhanced the cytokine-induced killer (CIK) cells-mediated killing. Here, using docetaxel, one of the most widely used cancer chemotherapeutic agents, as a model, we examined the molecular and immunogenic consequences of chemotherapeutic agent exposure in lung adenocarcinoma cell SPC-A1 cells...
February 2015: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Qingzhong He, Haisong Zhang, Youzhao Wang, Hong Hoi Ting, Wenhua Yu, Xuetao Cao, Wei Ge
BACKGROUND: Chemical crosslinking is the most straightforward method to produce bispecific antibodies (BsAb) for arming ex vivo activated cytotoxic T lymphocytes. However, heterogeneous polymers are produced by chemical crosslinking. Currently, it is not known under what circumstances or to what extent further purification is needed. RESULTS: In this study, we purified Traut's Reagent-Sulfo-SMCC crosslinked anti-CD3 × anti-HER2 by size-exclusion column chromatography and compared the capacity of the crude and the purified forms of the BsAb in enhancing cytokine-induced killer (CIK) cell-mediated cytotoxicity in vitro...
2014: Cell & Bioscience
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