Christine C Wu, Kristine A Tsantilas, Jea Park, Deanna Plubell, Justin A Sanders, Previn Naicker, Ireshyn Govender, Sindisiwe Buthelezi, Stoyan Stoychev, Justin Jordaan, Gennifer Merrihew, Eric Huang, Edward D Parker, Michael Riffle, Andrew N Hoofnagle, William S Noble, Kathleen L Poston, Thomas J Montine, Michael J MacCoss
Membrane-bound particles in plasma are composed of exosomes, microvesicles, and apoptotic bodies and represent ∼1-2% of the total protein composition. Proteomic interrogation of this subset of plasma proteins augments the representation of tissue-specific proteins, representing a "liquid biopsy," while enabling the detection of proteins that would otherwise be beyond the dynamic range of liquid chromatography-tandem mass spectrometry of unfractionated plasma. We have developed an enrichment strategy (Mag-Net) using hyper-porous strong-anion exchange magnetic microparticles to sieve membrane-bound particles from plasma...
April 2, 2024: bioRxiv