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https://www.readbyqxmd.com/read/29031081/optimization-of-the-promega-powerseq%C3%A2-auto-y-system-for-efficient-integration-within-a-forensic-dna-laboratory
#1
E A Montano, J M Bush, A M Garver, M M Larijani, S M Wiechman, C H Baker, M R Wilson, R A Guerrieri, E A Benzinger, D N Gehres, M L Dickens
The application of massively parallel sequencing (MPS) is growing in the forensic DNA field, as forensic DNA laboratories are continuously seeking methods to gain information from a limited or degraded forensic sample. However, the laborious nature of current MPS methodologies required for successful library preparation and sequencing leave opportunities for improvement to make MPS a practical option for processing forensic casework. In this study, the Promega PowerSeq™ Auto/Y System Prototype, a MPS laboratory workflow that incorporates multiplex amplification, was selected for optimization with the objectives to introduce automation for relieving manual processing, and to reduce the number of steps recommended by the standard protocol...
October 5, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29028566/concept-mapping-to-promote-meaningful-learning-help-relate-theory-to-practice-and-improve-learning-self-efficacy-in-asian-mental-health-nursing-students-a-mixed-methods-pilot-study
#2
Daniel T Bressington, Wai-Kit Wong, Kar Kei Claire Lam, Wai Tong Chien
OBJECTIVES: Student nurses are provided with a great deal of knowledge within university, but they can find it difficult to relate theory to nursing practice. This study aimed to test the appropriateness and feasibility of assessing Novak's concept mapping as an educational strategy to strengthen the theory-practice link, encourage meaningful learning and enhance learning self-efficacy in nursing students. DESIGN: This pilot study utilised a mixed-methods quasi-experimental design...
October 5, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/29028201/summed-area-technique-for-cube-maps
#3
Yi Xiao, Tze-Yui Ho, Chi-Sing Leung
The original Summed Area Table (SAT) structure is designed for handling 2D rectangular data. Due to the nature of spherical functions, the SAT structure cannot handle cube maps directly. This paper proposes a new SAT structure for cube maps and develops the corresponding lookup algorithm. Our formulation starts by considering a cube map as part of an auxiliary 3D function defined in a 3D rectangular space. We interpret the 2D integration process over the cube map surface as a 3D integration over the auxiliary 3D function...
October 11, 2017: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/29028102/the-ten-commandments-of-hepatic-radioembolization-expert-discussion-and-report-from-mediterranean-interventional-oncology-miolive-congress-2017
#4
J L Bilbao, R Iezzi, S N Goldberg, A Sami, O Akhan, F Giuliante, M Pompili, L Crocetti, K Malagari, V Valentini, A Gasbarrini, C Colosimo, R Manfredi
Microsphere and particle technology represent the next-generation agents that have formed the basis of interventional oncology, an evolving subspecialty of interventional radiology. One of these platforms, yttrium-90 microspheres, is increasingly being used as a treatment modality for primary and secondary liver tumors. Due to the widespread use of radioembolization, a comprehensive review of the methodologic and technical considerations seems to be mandatory. This article summarizes the expert discussion and report from Mediterranean Interventional Oncology Live Congress (MIOLive 2017) that was held in Rome, Italy, integrating evidence-reported literature and experience-based perceptions, to assist not only residents and fellows who are training in interventional radiologists but also practicing colleagues who are approaching to this intra-arterial treatment...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29028001/soil-protist-communities-form-a-dynamic-hub-in-the-soil-microbiome
#5
Wu Xiong, Alexandre Jousset, Sai Guo, Ida Karlsson, Qingyun Zhao, Huasong Wu, George A Kowalchuk, Qirong Shen, Rong Li, Stefan Geisen
Soil microbes are essential for soil fertility. However, most studies focus on bacterial and/or fungal communities, while the top-down drivers of this microbiome composition, protists, remain poorly understood. Here, we investigated how soil amendments affect protist communities and inferred potential interactions with bacteria and fungi. Specific fertilization treatments impacted both the structure and function of protist communities. Organic fertilizer amendment strongly reduced the relative abundance of plant pathogenic protists and increased bacterivorous and omnivorous protists...
October 13, 2017: ISME Journal
https://www.readbyqxmd.com/read/29027806/annals-express-clinical-evaluation-and-treatment-of-phaeochromocytoma
#6
Andrew Stuart Davison, Danielle Jones, Stuart Ruthven, Timothy Helliwell, Susannah L Shore
Phaeochromocytoma and extra adrenal paraganglioma are rare neuroendocrine tumours and have the potential to secrete adrenaline, noradrenaline and dopamine causing a myriad of clinical symptoms. Prompt diagnosis is essential for clinicians and requires a multi-disciplinary specialist approach for the clinical and laboratory investigation, diagnosis, treatment and follow-up of patients. This paper is an integrated review of the clinical and laboratory evaluation and treatment of patients suspected to have phaeochromocytoma or paraganglioma, highlighting recent developments and best practices from recent published clinical guidelines...
January 1, 2017: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/29027783/antibacterial-structural-color-hydrogel
#7
Zhuoyue Chen, Min Mo, Fanfan Fu, Luoran Shang, Huan Wang, Cihui Liu, Yuanjin Zhao
Structural color hydrogels with lasting survivability are important for many areas, while their anti-biodegradation capability is still lacking. Thus, we herein present novel antibacterial structural color hydrogels by simply synthesizing AgNPs in situ within the hydrogel materials. As the integrated AgNPs possessed wide and excellent antibacterial abilities, the structural color hydrogels could prevent bacteria adhering, avoid hydrogel damage, and maintain their vivid structural colors during application and storage...
October 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29027681/the-drivers-of-sea-lice-management-policies-and-how-best-to-integrate-them-into-a-risk-management-strategy-an-ecosystem-approach-to-sea-lice-management
#8
D Jackson, O Moberg, E M Stenevik Djupevåg, F Kane, H Hareide
The control of sea lice infestations on cultivated Atlantic salmon is a major issue in many regions of the world. The numerous drivers which shape the priorities and objectives of the control strategies vary for different regions/jurisdictions. These range from the animal welfare and economic priorities of the producers, to the mitigation of any potential impacts on wild stocks. Veterinary ethics, environmental impacts of therapeutants, and impacts for organic certification of the produce are, amongst others, additional sets of factors which should be considered...
October 13, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29027394/assessing-the-effect-of-guideline-introduction-on-clinical-practice-and-outcome-in-patients-with-endometrial-cancer-in-japan-a-project-of-the-japan-society-of-gynecologic-oncology-jsgo-guideline-evaluation-committee
#9
Shogo Shigeta, Satoru Nagase, Mikio Mikami, Masae Ikeda, Masako Shida, Isao Sakaguchi, Norichika Ushioda, Fumiaki Takahashi, Wataru Yamagami, Nobuo Yaegashi, Yasuhiro Udagawa, Hidetaka Katabuchi
OBJECTIVE: The Japan Society of Gynecologic Oncology (JSGO) published the first practice guideline for endometrial cancer in 2006. The JSGO guideline evaluation committee assessed the effect of this guideline introduction on clinical practice and patient outcome using data provided by the Japan Society of Obstetrics and Gynecology (JSOG) cancer registration system. METHODS: Data of patients with endometrial cancer registered between 2000 and 2012 were analyzed, and epidemiological and clinical trends were assessed...
November 2017: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29027094/multimodal-functional-neuroimaging-by-simultaneous-bold-fmri-and-fiber-optic-calcium-recordings-and-optogenetic-control
#10
REVIEW
Franziska Albers, Lydia Wachsmuth, Timo Mauritz van Alst, Cornelius Faber
Recent developments of optogenetic tools and fluorescence-based calcium recording techniques enable the manipulation and monitoring of neural circuits on a cellular level. Non-invasive imaging of brain networks, however, requires the application of methods such as blood oxygen level-dependent (BOLD) functional magnetic resonance imaging (fMRI), which is commonly used for functional neuroimaging. While BOLD fMRI provides brain-wide non-invasive reading of the hemodynamic response, it is only an indirect measure of neural activity...
October 12, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29027089/a-decade-of-a-thrips-invasion-in-china-lessons-learned
#11
Shengyong Wu, Liangde Tang, Xingrui Zhang, Zhenlong Xing, Zhongren Lei, Yulin Gao
The Western flower thrips, Frankliniella occidentalis Pergande (Thysanoptera: Thripidae) is an invasive polyphagous pest with an expanding global range that damages a wide variety of crops. F. occidentalis was first reported in China from Yunnan province during 2000, and has rapidly expanded its range since then. It is currently distributed across at least 10 provinces in China and has become a particularly devastating pest, causing substantial damage and economic losses. At present, the still heavy reliance on frequent use of insecticides for control of F...
October 11, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/29026639/optimising-the-acceptability-and-feasibility-of-novel-complex-interventions-an-iterative-person-based-approach-to-developing-the-uk-morita-therapy-outpatient-protocol
#12
Holly Victoria Rose Sugg, David A Richards, Julia Frost
BACKGROUND: The aim of this paper is to showcase best practice in intervention development by illustrating a systematic, iterative, person-based approach to optimising intervention acceptability and feasibility, as applied to the cross-cultural adaptation of Morita therapy for depression and anxiety. METHODS: We developed the UK Morita therapy outpatient protocol over four stages integrating literature synthesis and qualitative research. Firstly, we conducted in-depth interviews combining qualitative and cognitive interviewing techniques, utilising vignettes of Morita therapy being delivered and analysed using Framework analysis to investigate potential patients and therapists' perceptions of Morita therapy...
2017: Pilot and Feasibility Studies
https://www.readbyqxmd.com/read/29026454/opportunities-and-obstacles-using-a-clinical-decision-support-system-for-maternal-care-in-burkina-faso
#13
S Alphonse Zakane, Lars L Gustafsson, Ali Sie, Göran Tomson, Svetla Loukanova, Pia Bastholm-Rahmner
OBJECTIVE: Maternal and neonatal mortality is high in sub-Saharan Africa. To support Healthcare Workers (HCWs), a computerized decision support system (CDSS) was piloted at six rural maternal care units in Burkina Faso. During the two years of the study period, it was apparent from reports that the CDSS was not used regularly in clinical practice. This study aimed to explore the reasons why HCWs failed to use the CDSS. METHODS: A workshop, organized as group discussions and a plenary session, was performed with 13 participants to understand their experience with the CDSS and suggest improvements if pertinent...
2017: Online Journal of Public Health Informatics
https://www.readbyqxmd.com/read/29025453/interprofessional-communication-in-organ-transplantation-in-gauteng-province-south-africa
#14
Harriet Rosanne Etheredge, Claire Penn, Jennifer Watermeyer
BACKGROUND: Communication is essential to the transplant process, but it is challenging in South Africa (SA) because of the complexity of the country and the health system, the nature of transplantation as a technical procedure with inherent psychological considerations, and the large number of professionals involved. Transplant communication has not been explored in SA, and this study was the first to use health communication methods to generate empirical evidence relating to it. OBJECTIVE: To explore communication in transplant settings in Gauteng Province, SA...
June 30, 2017: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/29025360/best-practice-of-identification-and-proteomic-analysis-of-extracellular-vesicles-in-human-health-and-disease
#15
Barbara W Sódar, Árpád Kovács, Tamás Visnovitz, Éva Pállinger, Károly Vékey, Gabriella Pocsfalvi, Lilla Turiák, Edit I Buzás
Extracellular vesicles are emerging sources of biomarkers for modern preventive and precision medicine. Extracellular vesicles in body fluids offer a unique opportunity for integrative biomarker approaches due to their complex biocargo that includes proteins, lipids, nucleic acids and metabolites. Mass spectrometry-based proteomics data suggest that a significant portion of human proteins are sorted into extracellular vesicles and amenable for biomarker discovery schemes. Areas covered: this review focuses on key aspects of isolation, quality control and subsequent analysis of blood plasma- and conditioned medium-derived extracellular vesicle proteins, and summarizes the current state-of-the-art in the field...
October 13, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/29025070/interdisciplinary-family-conferences-to-improve-patient-experience-in-the-neonatal-intensive-care-unit
#16
Jennifer A Trujillo, Yesenia Fernandez, Lyla Ghafoori, Kristina Lok, Arwin Valencia
Parents play a significant role in the development of their neonate. They can affect the length of stay in a neonatal intensive care unit (NICU) and are seen as an integral part of the team. Parents are often put in positions where difficult decision making is required of them in the care of their critically ill child. Studies suggest that one way to improve the family's experience and to encourage their involvement is by establishing a formal and focused family conference. Therefore, the NICU social workers and the interdisciplinary team collaborated to formulate strategies to improve communication that would enhance current practice...
September 15, 2017: Health & Social Work
https://www.readbyqxmd.com/read/29024821/improving-the-efficiency-of-psychological-treatment-using-outcome-feedback-technology
#17
Jaime Delgadillo, Karen Overend, Mike Lucock, Martin Groom, Naomi Kirby, Dean McMillan, Simon Gilbody, Wolfgang Lutz, Julian A Rubel, Kim de Jong
AIMS: This study evaluated the impact of applying computerized outcome feedback (OF) technology in a stepped care psychological service offering low and high intensity therapies for depression and anxiety. METHODS: A group of therapists were trained to use OF based on routine outcome monitoring using depression (PHQ-9) and anxiety (GAD-7) measures. Therapists regularly reviewed expected treatment response graphs with patients and discussed cases that were "not on track" in clinical supervision...
September 28, 2017: Behaviour Research and Therapy
https://www.readbyqxmd.com/read/29024550/biology-of-obesity-and-weight-regain-implications-for-clinical-practice
#18
Mary Madeline Rogge, Bibha Gautam
BACKGROUND AND PURPOSE: Weight loss is recommended as first-line therapy for many chronic illnesses, including obesity. Most patients who do successfully lose weight are unable to maintain their reduced weight. Recent research findings are reviewed and synthesized to explain the biology of obesity, adaptation to weight loss, and weight regain. FINDINGS: Weight regain is a common consequence of successful weight loss. Current obesity management strategies fail to take into consideration the underlying genetic and environmental causes of obesity...
October 2017: Journal of the American Association of Nurse Practitioners
https://www.readbyqxmd.com/read/29024159/chemical-patterning-of-high-mobility-semiconducting-2d-bi2-o2-se-crystals-for-integrated-optoelectronic-devices
#19
Jinxiong Wu, Yujing Liu, Zhenjun Tan, Congwei Tan, Jianbo Yin, Tianran Li, Teng Tu, Hailin Peng
Patterning of high-mobility 2D semiconducting materials with unique layered structures and superb electronic properties offers great potential for batch fabrication and integration of next-generation electronic and optoelectronic devices. Here, a facile approach is used to achieve accurate patterning of 2D high-mobility semiconducting Bi2 O2 Se crystals using dilute H2 O2 and protonic mixture acid as efficient etchants. The 2D Bi2 O2 Se crystal after chemical etching maintains a high Hall mobility of over 200 cm(2) V(-1) s(-1) at room temperature...
October 12, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29023771/visibility-of-fiducial-markers-used-for-image-guided-radiation-therapy-on-optical-coherence-tomography-for-registration-with-ct-an-esophageal-phantom-study
#20
Pouya Jelvehgaran, Tanja Alderliesten, Jelmer J A Weda, Martijn de Bruin, Dirk J Faber, Maarten C C M Hulshof, Ton G van Leeuwen, Marcel van Herk, Johannes F de Boer
PURPOSE: Optical coherence tomography (OCT) is of interest to visualize microscopic esophageal tumor extensions to improve tumor delineation for radiation therapy (RT) planning. Fiducial marker placement is a common method to ensure target localization during planning and treatment. Visualization of these fiducial markers on OCT permits integrating OCT and computed tomography (CT) images used for RT planning via image registration. We studied the visibility of 13 (8 types) commercially available solid and liquid fiducial markers in OCT images at different depths using dedicated esophageal phantoms and evaluated marker placement depth in clinical practice...
October 11, 2017: Medical Physics
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