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https://www.readbyqxmd.com/read/28550347/correlation-between-different-scheimpflug-based-lens-densitometry-analysis-and-effective-phacoemulsification-time-in-mild-nuclear-cataracts
#1
Fernando Faria-Correia, Bernardo Lopes, Tiago Monteiro, Nuno Franqueira, Renato Ambrósio
PURPOSE: To assess the correlations between preoperative Scheimpflug-based lens densitometry and effective phacoemulsification time (EPT) in age-related nuclear cataracts. DESIGN: Retrospective consecutive study. METHODS: The setting was the Ophthalmology Department, Hospital de Braga, Portugal. The study population included 50 eyes (42 patients) with age-related nuclear cataracts submitted to uneventful phacoemulsification surgery. Different analysis methods of Scheimpflug-based lens densitometry were performed: Pentacam Nucleus Staging (PNS) score with an ordinal scale from 0 to 5 and three-dimensional (3D), linear and region of interest (ROI) methods, which are displayed on an absolute scale (from 0 to 100%)...
May 26, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28543640/electric-properties-tomography-biochemical-physical-and-technical-background-evaluation-and-clinical-applications
#2
REVIEW
Ulrich Katscher, Cornelius A T van den Berg
Electric properties tomography (EPT) derives the patient's electric properties, i.e. conductivity and permittivity, using standard magnetic resonance (MR) systems and standard MR sequences. Thus, EPT does not apply externally mounted electrodes, currents or radiofrequency (RF) probes, as is the case in competing techniques. EPT is quantitative MR, i.e. it yields absolute values of conductivity and permittivity. This review summarizes the physical equations underlying EPT, the corresponding basic and advanced reconstruction techniques and practical numerical aspects to realize these reconstruction techniques...
May 24, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/28531955/influence-of-sediment-chemistry-and-sediment-toxicity-on-macroinvertebrate-communities-across-99-wadable-streams-of-the-midwestern-usa
#3
Patrick W Moran, Lisa H Nowell, Nile E Kemble, Barbara J Mahler, Ian R Waite, Peter C Van Metre
Simultaneous assessment of sediment chemistry, sediment toxicity, and macroinvertebrate communities can provide multiple lines of evidence when investigating relations between sediment contaminants and ecological degradation. These three measures were evaluated at 99 wadable stream sites across 11 states in the Midwestern United States during the summer of 2013 to assess sediment pollution across a large agricultural landscape. This evaluation considers an extensive suite of sediment chemistry totaling 274 analytes (polycyclic aromatic hydrocarbons, organochlorine compounds, polychlorinated biphenyls, polybrominated diphenyl ethers, trace elements, and current-use pesticides) and a mixture assessment based on the ratios of detected compounds to available effects-based benchmarks...
May 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28530676/mutations-in-dzip1l-which-encodes-a-ciliary-transition-zone-protein-cause-autosomal-recessive-polycystic-kidney-disease
#4
Hao Lu, Maria C Rondón Galeano, Elisabeth Ott, Geraldine Kaeslin, P Jaya Kausalya, Carina Kramer, Nadina Ortiz-Brüchle, Nadescha Hilger, Vicki Metzis, Milan Hiersche, Shang Yew Tay, Robert Tunningley, Shubha Vij, Andrew D Courtney, Belinda Whittle, Elke Wühl, Udo Vester, Björn Hartleben, Steffen Neuber, Valeska Frank, Melissa H Little, Daniel Epting, Peter Papathanasiou, Andrew C Perkins, Graham D Wright, Walter Hunziker, Heon Yung Gee, Edgar A Otto, Klaus Zerres, Friedhelm Hildebrandt, Sudipto Roy, Carol Wicking, Carsten Bergmann
Autosomal recessive polycystic kidney disease (ARPKD), usually considered to be a genetically homogeneous disease caused by mutations in PKHD1, has been associated with ciliary dysfunction. Here, we describe mutations in DZIP1L, which encodes DAZ interacting protein 1-like, in patients with ARPKD. We further validated these findings through loss-of-function studies in mice and zebrafish. DZIP1L localizes to centrioles and to the distal ends of basal bodies, and interacts with septin2, a protein implicated in maintenance of the periciliary diffusion barrier at the ciliary transition zone...
May 22, 2017: Nature Genetics
https://www.readbyqxmd.com/read/28520986/chemical-ligation-of-oligonucleotides-using-an-electrophilic-phosphorothioester
#5
Hideto Maruyama, Ryota Oikawa, Mayu Hayakawa, Shono Takamori, Yasuaki Kimura, Naoko Abe, Genichiro Tsuji, Akira Matsuda, Satoshi Shuto, Yoshihiro Ito, Hiroshi Abe
We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonucleotides can be isolated, stored frozen, and used for the ligation reaction. The reaction of the EPT oligonucleotide and an amino-modified oligonucleotide took place without any extra reagents at pH 7.0-8.0 at room temperature, and resulted in a ligation product with a phosphoramidate bond with a 39-85% yield...
May 18, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28515200/impact-of-expedited-partner-therapy-ept-implementation-on-chlamydia-incidence-in-the-usa
#6
Okeoma Mmeje, Sarah Wallett, Giselle Kolenic, Jason Bell
OBJECTIVES: The diagnosis and treatment of Chlamydia trachomatis infection is important in preventing persistent or recurrent infection. Expedited partner therapy (EPT) is the favoured and supported method for STI treatment of the Centers for Disease Control and Prevention when the provider cannot be assured that all recent sexual partner(s) will seek therapy. EPT is legally permissible in 38 states and is endorsed by healthcare organisations to decrease the rates of chlamydia and gonorrhoea infection...
May 17, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28499260/metal-toxicity-affects-predatory-stream-invertebrates-less-than-other-functional-feeding-groups
#7
Matthias Liess, Nadine V Gerner, Ben J Kefford
Ecosystem effects of heavy metals need to be identified for a retrospective risk assessment, and potential impacts need to be predicted for a prospective risk assessment. In this study, we established a strong correlation between the toxic pressure of dissolved metals and invertebrate species. We compiled available data from a wide geographical range of Australian streams that were contaminated with heavy metals [mainly copper (Cu) and zinc (Zn)] and the corresponding invertebrate communities. Heavy metal toxicity is positively related to the proportion of predators within the invertebrate community, represented by the predatorratio, with an effect threshold range of 2...
May 9, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28482308/temporal-effects-of-enhanced-fine-sediment-loading-on-macroinvertebrate-community-structure-and-functional-traits
#8
Kate L Mathers, Stephen P Rice, Paul J Wood
Deposition of fine sediment that fills interstitial spaces in streambed substrates is widely acknowledged to have significant negative effects on macroinvertebrate communities, but the temporal consistency of clogging effects is less well known. In this study the effects of experimentally enhanced fine sediment content on aquatic invertebrates were examined over 126days in two lowland UK streams. Taxonomic approaches indicated significant differences in macroinvertebrate community structure associated with sediment treatment (clean or sedimented substrates), although the effects were variable on some occasions...
May 5, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28468648/expedited-partner-therapy-ept-increases-the-frequency-of-partner-notification-among-msm-in-lima-peru-a-pilot-randomized-controlled-trial
#9
Jesse L Clark, Eddy R Segura, Catherine E Oldenburg, Jessica Rios, Silvia M Montano, Amaya Perez-Brumer, Manuel Villaran, Jorge Sanchez, Thomas J Coates, Javier R Lama
BACKGROUND: Expedited Partner Therapy (EPT) has been shown to improve treatment outcomes among heterosexual partners of individuals with curable sexually transmitted infections (STIs). Although the use of EPT with men who have sex with men (MSM) has been debated, due to the potential for missed opportunities to diagnose unidentified cases of HIV and syphilis infection in symptomatic partners, increases in partner notification (PN) resulting from use of EPT may promote testing and treatment of otherwise unidentified partners...
May 4, 2017: BMC Medicine
https://www.readbyqxmd.com/read/28449274/a-dna-barcode-library-for-germany-s-mayflies-stoneflies-and-caddisflies-ephemeroptera-plecoptera-trichoptera
#10
Jérôme Morinière, Lars Hendrich, Michael Balke, Arne J Beermann, Tobias König, Monika Hess, Stefan Koch, Reinhard Müller, Florian Leese, Paul D N Hebert, Axel Hausmann, Christoph F Schubart, Gerhard Haszprunar
Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immature life stages, is a challenging, yet fundamental task. However, a comprehensive DNA barcode library based upon taxonomically well-curated specimens can overcome the problematic identification. Once available, this library will support the implementation of fast, cost-efficient, and reliable DNA-based identifications and assessments of ecological status...
April 27, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28426989/the-thermal-impact-of-subsurface-building-structures-on-urban-groundwater-resources-a-paradigmatic-example
#11
Jannis Epting, Stefan Scheidler, Annette Affolter, Paul Borer, Matthias H Mueller, Lukas Egli, Alejandro García-Gil, Peter Huggenberger
Shallow subsurface thermal regimes in urban areas are increasingly impacted by anthropogenic activities, which include infrastructure development like underground traffic lines as well as industrial and residential subsurface buildings. In combination with the progressive use of shallow geothermal energy systems, this results in the so-called subsurface urban heat island effect. This article emphasizes the importance of considering the thermal impact of subsurface structures, which commonly is underestimated due to missing information and of reliable subsurface temperature data...
April 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28426462/high-resolution-esophageal-manometry-interpretation-in-clinical-practice
#12
Rena Yadlapati
PURPOSE OF REVIEW: Esophageal high-resolution manometry (HRM) is the current state-of-the-art diagnostic tool to evaluate esophageal motility patterns and, as such, is widely adopted in clinical practice. This article will review the interpretation of esophageal HRM in clinical practice. RECENT FINDINGS: HRM uses a high-resolution catheter to transmit intraluminal pressure data that are subsequently converted into dynamic esophageal pressure topography (EPT) plots...
April 19, 2017: Current Opinion in Gastroenterology
https://www.readbyqxmd.com/read/28419841/buffered-1-lidocaine-with-epinephrine-can-be-as-effective-as-nonbuffered-2-lidocaine-with-epinephrine-for-maxillary-field-block
#13
James A Phero, Victor T Warren, Anson G Fisher, Eric M Rivera, Pooja T Saha, Glenn Reside, Ceib Phillips, Raymond P White
PURPOSE: Buffering local anesthetics with epinephrine (Epi) offers clinicians options not often considered. This study assessed outcomes for pulpal anesthesia, pain on injection, and time to midface numbness for buffered 1% lidocaine with 1:100,000 Epi versus nonbuffered 2% lidocaine with 1:100,000 Epi. MATERIALS AND METHODS: In this trial with a randomized, crossover design, buffered 1% lidocaine was compared with nonbuffered 2% lidocaine. Subjects were adult volunteers who served as their own controls...
March 25, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28419085/neonatal-outcomes-of-moderatelyly-preterm-infants-compared-to-extremely-preterm-infants
#14
Michele C Walsh, Edward F Bell, Sarah Kandefer, Shampa Saha, Waldemar A Carlo, Carl T D'Angio, Abbot R Laptook, Pablo J Sanchez, Barbara J Stoll, Seetha Shankaran, Krisa P Van Meurs, Noah Cook, Rosemary D Higgins, Abhik Das, Nancy S Newman, Kurt Schibler, Barbara Schmidt, C Michael Cotten, Brenda B Poindexter, Kristi L Watterberg, William E Truog
BACKGROUND: Extremely preterm infants (EPT, <29 weeks' gestation) represent only 0.9% of births in the U.S., yet these infants are focus of most published research. Moderately preterm neonates (MPT, 29-33(6/7) weeks), are an understudied group of high-risk infants. METHODS: Objective: To determine the neonatal outcomes of MPT across the gestational age spectrum, and to compare these to EPT. A prospective observational cohort was formed in 18 Level 3-4 NICUs in the Eunice Kennedy Shriver NICHD Neonatal Research Network...
April 18, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28404759/paving-the-way-for-dose-banding-of-chemotherapy-an-analytical-approach
#15
Heike Reinhardt, Rainer Trittler, Alison G Eggleton, Stefan Wöhrl, Thomas Epting, Marion Buck, Sabine Kaiser, Daniel Jonas, Justus Duyster, Manfred Jung, Martin J Hug, Monika Engelhardt
Background: In an interdepartmental cooperation, we investigated the feasibility and benefits of implementing dose banding of chemotherapy at our medical center. Based on this concept, chemotherapy doses are clustered into bands of similar dosage levels, thereby allowing the preproduction of frequently used standard doses of drugs, with sufficient physicochemical stability. Although established practice in the United Kingdom, there is little published evidence of its introduction elsewhere. Methods: We performed an analysis of local prescribing practice (22,310 chemotherapies) and identified gemcitabine, 5-fluorouracil, and carboplatin, among various others, as cytotoxic drugs suitable for dose banding...
April 2017: Journal of the National Comprehensive Cancer Network: JNCCN
https://www.readbyqxmd.com/read/28404485/state-level-gonorrhea-rates-and-expedited-partner-therapy-laws-insights-from-time-series-analyses
#16
K Owusu-Edusei, R Cramer, H W Chesson, T L Gift, J S Leichliter
OBJECTIVE: In this study, we examined state-level monthly gonorrhea morbidity and assessed the potential impact of existing expedited partner therapy (EPT) laws in relation to the time that the laws were enacted. STUDY DESIGN: Longitudinal study. METHODS: We obtained state-level monthly gonorrhea morbidity (number of cases/100,000 for males, females and total) from the national surveillance data. We used visual examination (of morbidity trends) and an autoregressive time series model in a panel format with intervention (interrupted time series) analysis to assess the impact of state EPT laws based on the months in which the laws were enacted...
March 24, 2017: Public Health
https://www.readbyqxmd.com/read/28401394/revisiting-inland-hypoxia-diverse-exceedances-of-dissolved-oxygen-thresholds-for-freshwater-aquatic-life
#17
Gavin N Saari, Zhen Wang, Bryan W Brooks
Water resources in many regions are stressed by impairments resulting from climate change, population growth and urbanization. In the United States (US), water quality criteria (WQC) and standards (WQS) were established to protect surface waters and associated designated uses, including aquatic life. In inland waters of the south central US, for example, depressed dissolved oxygen (DO) consistently results in impaired aquatic systems due to noncompliance with DO WQC and WQS. In the present study, we systematically examined currently available DO threshold data for freshwater fish and invertebrates and performed probabilistic aquatic hazard assessments with low DO toxicity data that were used to derive the US Environmental Protection Agency's (EPA) Ambient Water Quality Criteria (AWQC) for DO and newly published information...
April 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28366369/intraindividual-comparison-between-femtosecond-laser-assisted-and-conventional-cataract-surgery
#18
Anna S Mursch-Edlmayr, Matthias Bolz, Nikolaus Luft, Michael Ring, Thomas Kreutzer, Christoph Ortner, Matthias Rohleder, Siegfried G Priglinger
PURPOSE: To compare the safety and efficacy between femtosecond laser-assisted cataract surgery using the Victus laser system and conventional cataract surgery. SETTING: Department of Ophthalmology, Kepler University Hospital, Linz, Austria. DESIGN: Prospective randomized case series. METHODS: Both eyes of patients with age-related cataract were randomized to conventional cataract surgery or femtosecond laser-assisted cataract surgery, both with intraocular lens (IOL) implantation...
February 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28341232/lower-urinary-tract-symptoms-and-sexual-functions-after-endorectal-pull-through-for-hirschsprung-disease-controlled-long-term-outcomes
#19
Malla Neuvonen, Kristiina Kyrklund, Seppo Taskinen, Antti Koivusalo, Risto J Rintala, Mikko P Pakarinen
BACKGROUND/PURPOSE: To define the prevalence of lower urinary tract symptoms (LUTS) and outcomes for sexual function after endorectal pull-through (EPT) for Hirschsprung disease (HD) compared to controls. To date, similar controlled studies are lacking. METHODS: Patients aged ≥4years (n=123) operated on for HD at our center between 1987 and 2011 were invited to answer questionnaires on LUTS and sexual function (aged ≥16years). Patients with an intellectual disability and patients with a definitive endostomy were excluded...
March 6, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28340479/additive-effects-prevail-the-response-of-biota-to-multiple-stressors-in-an-intensively-monitored-watershed
#20
Alexander Gieswein, Daniel Hering, Christian K Feld
Freshwater ecosystems are impacted by a range of stressors arising from diverse human-caused land and water uses. Identifying the relative importance of single stressors and understanding how multiple stressors interact and jointly affect biology is crucial for River Basin Management. This study addressed multiple human-induced stressors and their effects on the aquatic flora and fauna based on data from standard WFD monitoring schemes. For altogether 1095 sites within a mountainous catchment, we used 12 stressor variables covering three different stressor groups: riparian land use, physical habitat quality and nutrient enrichment...
March 21, 2017: Science of the Total Environment
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