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3D printed scaffold

Clarisse Ribeiro, Carlos M Costa, Daniela M Correia, João Nunes-Pereira, Juliana Oliveira, Pedro Martins, Renato Gonçalves, Vanessa F Cardoso, Senentxu Lanceros-Méndez
Poly(vinylidene fluoride) (PVDF) and its copolymers are the polymers with the highest dielectric constants and electroactive responses, including piezoelectric, pyroelectric and ferroelectric effects. This semicrystalline polymer can crystallize in five different forms, each related to a different chain conformation. Of these different phases, the β phase is the one with the highest dipolar moment and the highest piezoelectric response; therefore, it is the most interesting for a diverse range of applications...
April 2018: Nature Protocols
Chengpeng Chen, Alexandra D Townsend, Elizabeth A Hayter, Hannah M Birk, Scott A Sell, R Scott Martin
We present an insert-based approach to fabricate scalable and multiplexable microfluidic devices for 3D cell culture and integration with downstream detection modules. Laser-cut inserts with a layer of electrospun fibers are used as a scaffold for 3D cell culture, with the inserts being easily assembled in a 3D-printed fluidic device for flow-based studies. With this approach, the number and types of cells (on the inserts) in one fluidic device can be customized. Moreover, after an investigation (i.e., stimulation) under flowing conditions, the cell-laden inserts can be removed easily for subsequent studies including imaging and cell lysis...
March 14, 2018: Analytical and Bioanalytical Chemistry
Sourav Mandal, Susanne Meininger, Uwe Gbureck, Bikramjit Basu
One of the important aspects in 3D powder printing (3DPP) is the selection of binder for a specific material composition to produce scaffolds with desired microstructure and physico-chemical properties. To this end, a new powder-binder combination, namely tetracalcium phosphate (TTCP) and phytic acid (IP6) was investigated at ambient temperature, for low load bearing application. A minimal deviation (<200 µm, w.r.t. computer aided design) was observed in the final sample through optimization of 3DPP process, along with minimum strut and macro-pore size of 200 and 750 µm, respectively...
March 8, 2018: Journal of Materials Science. Materials in Medicine
Yongzhou Wang, Ying Miao, Jieling Zhang, Jian Ping Wu, Thomas Brett Kirk, Jiake Xu, Dong Ma, Wei Xue
Hydrogels with shape memory behavior and internal structure have wide applications in fields ranging from tissue engineering and medical instruments to drug delivery; however, creating the hydrogels has proven to be extremely challenging. This study presents a three-dimensional (3D) printing technology to fabricate the shape memory hydrogels with internal structure (SMHs) by combining sodium alginate (alginate) and pluronic F127 diacrylate macromer (F127DA). SMHs were constituted by a dual network structure...
March 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Maria Touri, Fathollah Moztarzadeh, Noor Azuan Abu Osman, Mohammad Mehdi Dehghan, Masoud Mozafari
Tissue engineering scaffolds with oxygen generating elements have shown to be able to increase the level of oxygen and cell survivability in specific conditions. In this study, biphasic calcium phosphate (BCP) scaffolds with the composition of 60% hydroxyapatite (HA) and 40% beta-tricalcium phosphate (β-TCP), which have shown a great potential for bone tissue engineering applications, were fabricated by a direct-write assembly (robocasting) technique. Then, the three-dimensional (3D)-printed scaffolds were coated with different ratios of an oxygen releasing agent, calcium peroxide (CPO), which encapsulated within a polycaprolactone (PCL) matrix through dip-coating, and used for in situ production of oxygen in the implanted sites...
March 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Hyeongjin Lee, Gi Hoon Yang, Minseong Kim, JaeYoon Lee, JunTae Huh, GeunHyung Kim
Biomaterials must be biocompatible, biodegradable, and mechanically stable to be used for tissue engineering applications. Among various biomaterials, a natural-based biopolymer, collagen, has been widely applied in tissue engineering because of its outstanding biocompatibility. However, due to its low mechanical properties, collagen has been a challenge to build a desired/complex 3D porous structure with appropriate mechanical strength. To overcome this problem, in this study, we used a low temperature printing process to create a 3D porous scaffold consisting of collagen, decellularized extracellular matrix (dECM) to induce high cellular activities, and silk-fibroin (SF) to attain the proper mechanical strength...
March 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J Van Wijnen, Michael J Yaszemski, Sanjeev Kakar
PURPOSE: Revascularization of natural and synthetic scaffolds is a critical part of the scaffold's incorporation and tissue ingrowth. Our goals were to create a biocompatible polymer scaffold with 3D-printing technology, capable of sustaining vascularization and tissue ingrowth. METHODS: We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow tissue ingrowth via large interconnected pores. The scaffolds were prepared with Poly(lactic-co-glycolic acid)(PLGA) microspheres seeded with or without different growth factors including VEGF,FGF-2, and/or BMP-2...
March 7, 2018: Connective Tissue Research
Bo Yin, Bingjian Xue, Zhihong Wu, Jiguang Ma, Keming Wang
The aim of this study was to explore an innovative method to improve the osteogenic ability of porous titanium. We used gelatin (Gel) and nano-hydroxyapatite (nHA) to construct micro-scaffolds within the pores of porous titanium alloy. We compared three groups: control, Gel:nHA = 1:0, and Gel:nHA = 1:1. We assessed cell attachment, cell proliferation, and osteogenic (alkaline phosphatase [ALP] and collagen type 1 [Col-1]) and cytoskeletal (Talin) gene and protein expression in MC3T3-E1 cells. We also evaluated osteogenic abilities in a rabbit calvarial defect model...
2018: American Journal of Translational Research
Shanshan Wei, Gang Qu, Guanyi Luo, Yuxing Huang, Huisheng Zhang, Xuechang Zhou, Liqiu Wang, Zhou Liu, Tiantian Kong
Creating complex three-dimensional structures from soft yet durable materials enables advances in fields, such as flexible electronics, regenerating tissue engineering and soft robotics. The tough-hydrogels that mimic the human skin can bear enormous mechanical loads. By employing a spider-inspired biomimetic microfluidic-nozzle, we successfully achieve the continuous-printing of tough-hydrogels into fibers, 2D networks and even 3D structures without compromising its extreme mechanical properties. The resultant thin fibers demonstrate a stretch up to 21 at a water content of 52%, and are intrinsically transparent, biocompatible, and conductive at high stretches...
March 5, 2018: ACS Applied Materials & Interfaces
Woojin Cho, Alan Varkey Job, Jing Chen, Jung Hwan Baek
Three-dimensional (3D) printing is a transformative technology with a potentially wide range of applications in the field of orthopaedic spine surgery. This article aims to review the current applications, limitations, and future developments of 3D printing technology in orthopaedic spine surgery. Current preoperative applications of 3D printing include construction of complex 3D anatomic models for improved visual understanding, preoperative surgical planning, and surgical simulations for resident education...
February 2018: Asian Spine Journal
Udayabhanu Jammalamadaka, Karthik Tappa
Three-dimensional printing has significant potential as a fabrication method in creating scaffolds for tissue engineering. The applications of 3D printing in the field of regenerative medicine and tissue engineering are limited by the variety of biomaterials that can be used in this technology. Many researchers have developed novel biomaterials and compositions to enable their use in 3D printing methods. The advantages of fabricating scaffolds using 3D printing are numerous, including the ability to create complex geometries, porosities, co-culture of multiple cells, and incorporate growth factors...
March 1, 2018: Journal of Functional Biomaterials
Wenyang Xu, Andrey Pranovich, Peter Uppstu, Xiaoju Wang, Dennis Kronlund, Jarl Hemming, Heidi Öblom, Niko Moritz, Maren Preis, Niklas Sandler, Stefan Willför, Chunlin Xu
Hemicelluloses, the second most abundant polysaccharide right after cellulose, are in practice still treated as a side-stream in biomass processing industries. In the present study, we report an approach to use a wood-derived and side-stream biopolymer, spruce wood hemicellulose (galactoglucomannan, GGM) to partially replace the synthetic PLA as feedstock material in 3D printing. A solvent blending approach was developed to ensure the even distribution of the formed binary biocomposites. The blends of hemicellulose and PLA with varied ratio up to 25% of hemicellulose were extruded into filaments by hot melt extrusion...
May 1, 2018: Carbohydrate Polymers
João C Boga, Sónia P Miguel, Duarte de Melo-Diogo, António G Mendonça, Ricardo O Louro, Ilídio J Correia
The incidence of fractures and bone-related diseases like osteoporosis has been increasing due to aging of the world's population. Up to now, grafts and titanium implants have been the principal therapeutic approaches used for bone repair/regeneration. However, these types of treatment have several shortcomings, like limited availability, risk of donor-to-recipient infection and tissue morbidity. To overcome these handicaps, new 3D templates, capable of replicating the features of the native tissue, are currently being developed by researchers from the area of tissue engineering...
February 21, 2018: Colloids and Surfaces. B, Biointerfaces
Aden Díaz Nocera, Romina Comín, Nancy Alicia Salvatierra, Mariana Paula Cid
Collagen is widely used in tissue engineering because it can be extracted in large quantities, and has excellent biocompatibility, good biodegradability, and weak antigenicity. In the present study, we isolated printable collagen from bovine Achilles tendon and examined the purity of the isolated collagen using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The bands obtained corresponded to α1 , α2 and β chains with little contamination from other small proteins. Furthermore, rheological measurements of collagen dispersions (60 mg per ml of PBS) at pH 7 revealed values of viscosity of 35...
February 27, 2018: Biomedical Microdevices
Bruna Nunes Teixeira, Paola Aprile, Roberta H Mendonça, Daniel J Kelly, Rossana Mara da Silva Moreira Thiré
The majority of synthetic polymers used in 3 D printing are not designed to promote specific cellular interactions and hence possess limited bioactivity. Most of the strategies proposed to overcome this limitation demand multiple and expensive processing steps. This study aimed to evaluate the surface modification of 3D-printed poly(lactic acid) (PLA) scaffolds with polydopamine (PDA) coating as an alternative strategy to enhance their bioactivity and to facilitate the immobilization of type I collagen (COL I) onto the implant surface...
February 26, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Jae Yoon Lee, YoungWon Koo, GeunHyung Kim
This work investigated the printability and applicability of a core/shell cell-printed scaffold for medium-term (for up to 20 days) cryopreservation and subsequent cultivation with acceptable cellular activities including cell viability. We developed an innovative cell printing process supplemented with a microfluidic channel, a core/shell nozzle, and low temperature working stage to obtain a cell-laden 3D porous collagen scaffold for cryopreservation. The 3D porous biomedical scaffold consisted of core/shell struts with a cell-laden collagen-based bioink/dimethyl sulfoxide mixture in the core-region, and an alginate/poly(ethylene oxide) mixture in the shell-region...
February 23, 2018: ACS Applied Materials & Interfaces
Tobias Dahlberg, Tim Stangner, Hanqing Zhang, Krister Wiklund, Petter Lundberg, Ludvig Edman, Magnus Andersson
We report a novel method for fabrication of three-dimensional (3D) biocompatible micro-fluidic flow chambers in polydimethylsiloxane (PDMS) by 3D-printing water-soluble polyvinyl alcohol (PVA) filaments as master scaffolds. The scaffolds are first embedded in the PDMS and later residue-free dissolved in water leaving an inscription of the scaffolds in the hardened PDMS. We demonstrate the strength of our method using a regular, cheap 3D printer, and evaluate the inscription process and the channels micro-fluidic properties using image analysis and digital holographic microscopy...
February 20, 2018: Scientific Reports
David Chimene, Charles W Peak, James Gentry, James K Carrow, Lauren M Cross, Eli Mondragon, Guinea B C Cardoso, Roland Kaunas, Akhilesh K Gaharwar
We introduce an enhanced nanoengineered ionic covalent entanglement (NICE) bioink for the fabrication of mechanically stiff and elastomeric 3D biostructures. NICE bioink formulations combine nanocomposite and ionic covalent entanglement (ICE) strengthening mechanisms to print customizable cell-laden constructs for tissue engineering with high structural fidelity and mechanical stiffness. Nanocomposite and ICE strengthening mechanisms complement each other through their synergistic interactions, improving mechanical strength, elasticity, toughness, and flow properties beyond the sum of the effects of both reinforcement techniques alone...
February 20, 2018: ACS Applied Materials & Interfaces
Xuan Zhou, Haitao Cui, Margaret Nowicki, Shida Miao, Se-Jun Lee, Fahed Masood, Brent T Harris, Lijie Grace Zhang
Central nerve repair and regeneration remain challenging problems worldwide largely because of the extremely weak inherent regenerative capacity and accompanying fibrosis of native nerves. Inadequate solutions to unmet needs for clinical therapeutics encourage the development of novel strategies to promote nerve regeneration. Recently, 3D bioprinting techniques, as one of a set of valuable tissue engineering technologies, have shown great promise toward fabricating complex and customizable artificial tissue scaffolds...
February 20, 2018: ACS Applied Materials & Interfaces
Yuanyuan Xu, Xiao Guo, Shuaitao Yang, Long Li, Peng Zhang, Wei Sun, Changyong Liu, Shengli Mi
Articular cartilage (AC) has gradient features in both mechanics and histology as well as a poor regeneration ability. The repair of AC poses difficulties in both research and the clinic. In this paper, a gradient scaffold based on PLGA-ECM was proposed. Cartilage scaffolds with a three-layer gradient structure were fabricated by PLGA through 3D printing, and the microstructure orientation and pore fabrication were made by dECM injection and directional freezing. The manufactured scaffold has a mechanical strength close to that of real cartilage...
February 20, 2018: Journal of Biomedical Materials Research. Part A
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