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3D printed scaffold

Cheng Zhong, Hai-Yang Xie, Lin Zhou, Xiao Xu, Shu-Sen Zheng
BACKGROUND: Because of an increasing discrepancy between the number of potential liver graft recipients and the number of organs available, scientists are trying to create artificial liver to mimic normal liver function and therefore, to support the patient's liver when in dysfunction. 3D printing technique meets this purpose. The present study was to test the feasibility of 3D hydrogel scaffolds for liver engineering. METHODS: We fabricated 3D hydrogel scaffolds with a bioprinter...
October 2016: Hepatobiliary & Pancreatic Diseases International: HBPD INT
Lisa Elviri, Annalisa Bianchera, Carlo Bergonzi, Ruggero Bettini
The main target of tissue engineering is the preparation and application of adequate materials for the design and production of scaffolds, that possess properties promoting cell adhesion, proliferation and differentiation. The use of natural polysaccharides, such as chitosan, to prepare hydrogels for wound healing and controlled drug delivery is a research topic of wide and increasing interest. Areas covered: This review presents the latest results and challenges in the preparation of chitosan and chitosan-based scaffold/hydrogel for wound healing applications...
October 12, 2016: Expert Opinion on Drug Delivery
Hongshi Ma, Jian Luo, Zhe Sun, Lunguo Xia, Mengchao Shi, Mingyao Liu, Jiang Chang, Chengtie Wu
Primary bone cancer brings patients great sufferings. To deal with the bone defects resulted from cancer surgery, biomaterials with good bone-forming ability are necessary to repair bone defects. Meanwhile, in order to prevent possible tumor recurrence, it is essential that the remaining tumor cells around bone defects are completely killed. However, there are few biomaterials with the ability of both cancer therapy and bone regeneration until now. Here, we fabricated a 3D-printed bioceramic scaffold with a uniformly self-assembled Ca-P/polydopamine nanolayer surface...
December 2016: Biomaterials
Nathalie Nielsen, Christoffer Laustsen, Lotte Bonde Bertelsen
Endothelial progenitor cells (EPCs) represent a heterogeneous cell population that is believed to be involved in vasculogenesis. With the purpose of enhancing endothelial repair, EPCs could have a potential for future cell therapies. Due to the low amount of EPCs in the peripheral circulating blood, in vitro expansion is needed before administration to recipients and the effects of in vitro culturing is still an under-evaluated field with little knowledge of how the cells change over time in culture. The aim of this study was to use hyperpolarised carbon-13 magnetic resonance spectroscopy to profile important metabolic pathways in a population of progenitor cells and to show that cell culturing in 3D scaffolds seem to block the metabolic processes that leads to cell senescence...
October 5, 2016: Experimental Cell Research
Yi-Cheun Yeh, Christopher B Highley, Liliang Ouyang, Jason A Burdick
Three-dimensional (3D) printed scaffolds have great potential in biomedicine; however, it is important that we are able to design such scaffolds with a range of diverse properties towards specific applications. Here, we report the extrusion-based 3D printing of biodegradable and photocurable acrylated polyglycerol sebacate (Acr-PGS) to fabricate scaffolds with elastic properties. Two Acr-PGS macromers were synthesized with varied molecular weights and viscosity, which were then blended to obtain photocurable macromer inks with a range of viscosities...
October 7, 2016: Biofabrication
Ying Yang, Shengbing Yang, Yugang Wang, Zhifeng Yu, Haiyong Ao, Hongbo Zhang, Ling Qin, Olivier Guillaume, David Eglin, R Geoff Richards, Tingting Tang
: Contaminated or infected bone defects remain serious challenges in clinical trauma and orthopaedics, and a bone substitute with both osteoconductivity and antibacterial properties represents an improvement for treatment strategy. In this study, quaternized chitosan (hydroxypropyltrimethyl ammonium chloride chitosan, HACC) was grafted to 3D-printed scaffolds composed of polylactide-co-glycolide (PLGA) and hydroxyapatite (HA), in order to design bone engineering scaffolds endowed with antibacterial and osteoconductive properties...
September 26, 2016: Acta Biomaterialia
Sung Eun Kim, Young-Pil Yun, Kyu-Sik Shim, Hak-Jun Kim, Kyeongsoon Park, Hae-Ryong Song
The aim of this study was to evaluate the in vitro osteogenic effects and in vivo new bone formation of three-dimensional (3D) printed alendronate (Aln)-releasing poly(caprolactone) (PCL) (Aln/PCL) scaffolds in rat tibial defect models. 3D printed Aln/PCL scaffolds were fabricated via layer-by-layer deposition. The fabricated Aln/PCL scaffolds had high porosity and an interconnected pore structure and showed sustained Aln release. In vitro studies showed that MG-63 cells seeded on the Aln/PCL scaffolds displayed increased alkaline phosphatase (ALP) activity and calcium content in a dose-dependent manner when compared with cell cultures in PCL scaffolds...
2016: Biomedical Materials
Mian Wang, Pelagie Favi, Xiaoqian Cheng, Negar H Golshan, Katherine S Ziemer, Michael Keidar, Thomas J Webster
: Three-dimensional (3D) printing is a new fabrication method for tissue engineering which can precisely control scaffold architecture at the micron-scale. However, scaffolds not only need 3D biocompatible structures that mimic the micron structure of natural tissues, they also require mimicking of the nano-scale extracellular matrix properties of the tissue they intend to replace. In order to achieve this, the objective of the present in vitro study was to use cold atmospheric plasma (CAP) as a quick and inexpensive way to modify the nano-scale roughness and chemical composition of a 3D printed scaffold surface...
September 22, 2016: Acta Biomaterialia
Miao Sun, An Liu, Huifeng Shao, Xianyan Yang, Chiyuan Ma, Shigui Yan, Yanming Liu, Yong He, Zhongru Gou
Wollastonite (CaSiO3; CSi) ceramic is a promising bioactive material for bone defect repair due to slightly fast degradation of its porous constructs in vivo. In our previous strategy some key features of CSi ceramic have been significantly improved by dilute magnesium doping for regulating mechanical properties and biodegradation. Here we demonstrate that 6 ~ 14% of Ca substituted by Mg in CSi (CSi-Mgx, x = 6, 10, 14) can enhance the mechanical strength (>40 MPa) but not compromise biological performances of the 3D printed porous scaffolds with open porosity of 60‒63%...
2016: Scientific Reports
Elena Provaggi, Julian J H Leong, Deepak M Kalaskar
The latest and fastest-growing innovation in the medical field has been the advent of three-dimensional printing technologies, which have recently seen applications in the production of low-cost, patient-specific medical implants. While a wide range of three-dimensional printing systems has been explored in manufacturing anatomical models and devices for the medical setting, their applications are cutting-edge in the field of spinal surgery. This review aims to provide a comprehensive overview and classification of the current applications of three-dimensional printing technologies in spine care...
September 22, 2016: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
Liliang Ouyang, Rui Yao, Yu Zhao, Wei Sun
3D cell printing is an emerging technology for fabricating complex cell-laden constructs with precise and pre-designed geometry, structure and composition to overcome the limitations of 2D cell culture and conventional tissue engineering scaffold technology. This technology enables spatial manipulation of cells and biomaterials, also referred to as 'bioink', and thus allows study of cellular interactions in a 3D microenvironment and/or in the formation of functional tissues and organs. Recently, many efforts have been made to develop new bioinks and to apply more cell sources for better biocompatibility and biofunctionality...
2016: Biofabrication
Bin Xu, Darren Rodenhizer, Shakir Lakhani, Xiaoshu Zhang, John P Soleas, Laurie Ailles, Alison P McGuigan
In the past decade, it has been well recognised that the tumour microenvironment contains microenvironmental components such as hypoxia that significantly influence tumour cell behaviours such, invasiveness and therapy resistance, all of which provide new targets for studying cancer biology and developing anticancer therapeutics. In response, a large number of two-dimensional and three-dimensional (3D) in vitro tumour models have been developed to recapitulate different aspects of the tumour microenvironment and enable the study of related biological questions...
2016: Biofabrication
Kambiz Chizari, Mohamed Amine Daoud, Anil Raj Ravindran, Daniel Therriault
The utilization of 3D printing of highly conductive (σ ≈ 2350 S m(-1) ) polymer composite structures for the functional optimization of scaffold-shaped liquid sensors is demonstrated. This study can open the pathway of the application of 3D printing of conductive composites for optimization of structures useful for various applications such as smart sensors in textile or in the field of electronics.
September 14, 2016: Small
Christoph Rücker, Holger Kirch, Oliver Pullig, Heike Walles
Despite the great regenerative potential of human bone, large bone defects are a serious condition. Commonly, large defects are caused by trauma, bone disease, malignant tumor removal, and infection or medication-related osteonecrosis. Large defects necessitate clinical treatment in the form of autologous bone transplantation or implantation of biomaterials as well as the application of other available methods that enhance bone defect repair. The development and application of prevascularized bone implants are closely related to the development animal models and require dedicated methods in order to reliably predict possible clinical outcomes and the efficacy of implants...
2016: Current Molecular Biology Reports
Shuai Li, Yuan-Yuan Liu, Li-Jun Liu, Qing-Xi Hu
Despite considerable advances in tissue engineering over the past two decades, solutions to some crucial problems remain elusive. Vascularization is one of the most important factors that greatly influence the function of scaffolds. Many research studies have focused on the construction of a vascular-like network with prevascularization structure. Sacrificial materials are widely used to build perfusable vascular-like architectures, but most of these fabricated scaffolds only have a 2D plane-connected network...
September 28, 2016: ACS Applied Materials & Interfaces
Margaret A Nowicki, Nathan J Castro, Michael W Plesniak, Lijie Grace Zhang
Osteochondral tissue has a complex graded structure where biological, physiological, and mechanical properties vary significantly over the full thickness spanning from the subchondral bone region beneath the joint surface to the hyaline cartilage region at the joint surface. This presents a significant challenge for tissue-engineered structures addressing osteochondral defects. Fused deposition modeling (FDM) 3D bioprinters present a unique solution to this problem. The objective of this study is to use FDM-based 3D bioprinting and nanocrystalline hydroxyapatite for improved bone marrow human mesenchymal stem cell (hMSC) adhesion, growth, and osteochondral differentiation...
October 14, 2016: Nanotechnology
Sławomir Rumiński, Barbara Ostrowska, Jakub Jaroszewicz, Tomasz Skirecki, Krzysztof Włodarski, Wojciech Święszkowski, Małgorzata Lewandowska-Szumieł
The capacity of bone grafts to repair critical size defects can be greatly enhanced by the delivery of mesenchymal stem cells (MSCs). Adipose tissue is considered the most effective source of MSCs (ADSCs); however, the efficiency of bone regeneration using undifferentiated ADSCs is low. Therefore, in this study, we propose to use scaffolds based on polycaprolactone (PCL), which is widely considered a suitable MSC delivery system, as a 3D culture environment promoting osteogenic differentiation of ADSCs. PCL scaffolds enriched with 5% tricalcium phosphate (TCP) were used...
September 6, 2016: Journal of Tissue Engineering and Regenerative Medicine
Xuan Zhou, Nathan J Castro, Wei Zhu, Haitao Cui, Mitra Aliabouzar, Kausik Sarkar, Lijie Grace Zhang
3D printing and ultrasound techniques are showing great promise in the evolution of human musculoskeletal tissue repair and regeneration medicine. The uniqueness of the present study was to combine low intensity pulsed ultrasound (LIPUS) and advanced 3D printing techniques to synergistically improve growth and osteogenic differentiation of human mesenchymal stem cells (MSC). Specifically, polyethylene glycol diacrylate bioinks containing cell adhesive Arginine-Glycine-Aspartic acid-Serene (RGDS) peptide and/or nanocrystalline hydroxyapatite (nHA) were used to fabricate 3D scaffolds with different geometric patterns via novel table-top stereolithography 3D printer...
2016: Scientific Reports
WonJin Kim, Hyeongjin Lee, YongBok Kim, Chang Hyun Choi, DaeWeon Lee, Heon Hwang, GeunHyung Kim
In recent years, a variety of biomimetic hydrogel scaffolds have been used in tissue engineering because hydrogels can provide reasonable soft-tissue-like environmental conditions for various cell responses. However, although hydrogels can provide an outstanding biofunctional platform, their poor mechanical stability and low processability have been obstacles for their usage as biomedical scaffolds. To overcome this limitation, we propose a simple and versatile method using 3D printing supplemented with a low-temperature working plate and coating process to reinforce the mechanical properties and various cellular activities by accommodating the poly(ε-caprolactone) (PCL)...
2016: Biomedical Materials
Stellan Hertegård
PURPOSE OF REVIEW: Tissue engineering is a rapidly expanding field in medicine and involves regeneration and restoration of many organs, including larynx and the airways. Currently, this is not included in routine practice; however, a number of clinical trials in humans are ongoing or starting. This review will cover publications during the past 2 years and the focus is on larynx and trachea. RECENT FINDINGS: Recent reports concern the development and investigations of cell therapies, including biological factors such as growth factors which promote healing of damage and increased vascular support of the tissue...
August 31, 2016: Current Opinion in Otolaryngology & Head and Neck Surgery
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