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https://www.readbyqxmd.com/read/28424229/tension-activated-channels-in-the-mechanism-of-osmotic-fitness-in-pseudomonas-aeruginosa
#1
Uğur Çetiner, Ian Rowe, Anthony Schams, Christina Mayhew, Deanna Rubin, Andriy Anishkin, Sergei Sukharev
Pseudomonas aeruginosa (PA) is an opportunistic pathogen with an exceptional ability to adapt to a range of environments. Part of its adaptive potential is the ability to survive drastic osmolarity changes. Upon a sudden dilution of external medium, such as during exposure to rain, bacteria evade mechanical rupture by engaging tension-activated channels that act as osmolyte release valves. In this study, we compare fast osmotic permeability responses in suspensions of wild-type PA and Escherichia coli (EC) strains in stopped-flow experiments and provide electrophysiological descriptions of osmotic-release channels in PA...
April 19, 2017: Journal of General Physiology
https://www.readbyqxmd.com/read/28422915/enteral-tranexamic-acid-attenuates-vasopressor-resistance-and-changes-in-%C3%AE-1-adrenergic-receptor-expression-in-hemorrhagic-shock
#2
Marco Santamaria, Federico Aletti, Joyce B Li, Aaron Tan, Monica Chang, Jessica Leon, Geert W Schmid-Schönbein, Erik B Kistler
BACKGROUND: Irreversible hemorrhagic shock is characterized by hyporesponsiveness to vasopressor and fluid therapy. Little is known, however, about the mechanisms that contribute to this phenomenon. Previous studies have shown that decreased intestinal perfusion in hemorrhagic shock leads to proteolytically-mediated increases in gut permeability, with subsequent egress of vasoactive substances systemically. Maintenance of blood pressure is achieved in part by α1 receptor modulation, which may be affected by vasoactive factors; we thus hypothesized that decreases in hemodynamic stability and vasopressor response in shock can be prevented by enteral protease inhibition...
April 18, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28419508/experience-of-carrier-couples-identified-through-a-population-based-carrier-screening-pilot-program-for-four-founder-autosomal-recessive-diseases-in-saguenay-lac-saint-jean
#3
Jessica Tardif, Annabelle Pratte, Anne-Marie Laberge
A pilot population-based carrier screening program started in 2010 in the Saguenay-Lac-Saint-Jean region of Quebec, Canada for four recessive diseases with local founder effects (tyrosinemia type I, ARSACS, congenital lactic acidosis, and Andermann syndrome). OBJECTIVES: Describe the experience of carrier couples identified through this program. METHODS: Semi-structured interviews were performed with carrier couples. Thematic analysis of interview transcripts was done to identify emerging themes...
April 18, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28417555/a-novel-bat-shaped-dicyanomethylene-4h-pyran-functionalized-naphthalimidefor-highly-efficient-solution-processed-multilevel-memory-devices
#4
Jian-Mei Lu, Qi-Jian Zhang, Shi-Feng Miao, Hua Li, Jing-Hui He, Na-Jun Li, Qing-Feng Xu, Dong-Yun Chen
Small molecule based multilevel memory device has attracted increasing attentions since the advantages as super-high storage density, fast reading speed, light weight, low energy consumption, and shock resistance. However, the fabrication of small molecule-based device always needs expensive vacuum deposition techniques or high temperature for spin-coating. In this paper, through rationally tailoring of the previous molecule DPCNCANA, a novel bat-shaped A-D-A-type symmetric framework has been successfully synthesized, and can be dissolved in common solvents under room temperature...
April 18, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28417425/overexpression-of-oshsp18-0-ci-enhances-resistance-to-bacterial-leaf-streak-in-rice
#5
Yanhu Ju, Hongjuan Tian, Ruihua Zhang, Liping Zuo, Guixiu Jin, Qian Xu, Xinhua Ding, Xiangkui Li, Zhaohui Chu
BACKGROUND: The small heat shock proteins represent a large family of proteins that respond to a wide range of abiotic and biotic stresses. OsHsp18.0-CI confers tolerance to salt and cadmium and interacts with viral RNA-dependent RNA polymerase (RdRp). However, the direct function of OsHsp18.0-CI in resistance against biotic stresses remains unclear in rice. RESULTS: Here, we report that the expression of OsHsp18.0-CI was up-regulated upon inoculation with RS105, a strain of Xanthomonas oryzae pv...
December 2017: Rice
https://www.readbyqxmd.com/read/28416430/-extracorporeal-membrane-oxygenation-in-critically-ill-neonates-and-children
#6
J Rambaud, J Guilbert, I Guellec, S Jean, A Durandy, M Demoulin, A Amblard, R Carbajal, P-L Leger
Extracorporeal membrane oxygenation is used as a last resort during neonatal and pediatric resuscitation in case of refractory circulatory or respiratory failure under maximum conventional therapies. Different types of ECMO can be used depending on the initial failure. The main indications for ECMO are refractory respiratory failure (acute respiratory distress syndrome, status asthmaticus, severe pneumonia, meconium aspiration syndrome, pulmonary hypertension) and refractory circulatory failure (cardiogenic shock, septic shock, refractory cardiac arrest)...
April 14, 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/28416347/blood-collection-vials-and-clinically-used-intravenous-fluids-contain-significant-amounts-of-nitrite
#7
Luis E F Almeida, Sayuri Kamimura, Margaret Y Nettleton, Celia M de Souza Batista, Elizabeth Walek, Alfia Khaibullina, Li Wang, Zenaide M N Quezado
The biology of the inorganic anion nitrite is linked to nitric oxide (NO) as nitrite can be reduced to NO and mediate its biological activities. Thus, studies of nitrite biology require sensitive and selective chemical assays. The acetic and ascorbic acids method is selective for nitrite and measures it in biological matrices. However, one of the pitfalls of nitrite measurements is its ubiquitous presence in sample collection tubes. Here, we showed high levels of nitrite in collection tubes containing EDTA, sodium citrate or sodium heparin and smaller amounts in tubes containing lithium heparin or serum clot activator...
April 14, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28415375/nonlinear-monte-carlo-model-of-superdiffusive-shock-acceleration-with-magnetic-field-amplification
#8
Andrei M Bykov, Donald C Ellison, Sergei M Osipov
Fast collisionless shocks in cosmic plasmas convert their kinetic energy flow into the hot downstream thermal plasma with a substantial fraction of energy going into a broad spectrum of superthermal charged particles and magnetic fluctuations. The superthermal particles can penetrate into the shock upstream region producing an extended shock precursor. The cold upstream plasma flow is decelerated by the force provided by the superthermal particle pressure gradient. In high Mach number collisionless shocks, efficient particle acceleration is likely coupled with turbulent magnetic field amplification (MFA) generated by the anisotropic distribution of accelerated particles...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28415173/signal-optimization-in-urban-transport-a-totally-asymmetric-simple-exclusion-process-with-traffic-lights
#9
Chikashi Arita, M Ebrahim Foulaadvand, Ludger Santen
We consider the exclusion process on a ring with time-dependent defective bonds at which the hopping rate periodically switches between zero and one. This system models main roads in city traffics, intersecting with perpendicular streets. We explore basic properties of the system, in particular dependence of the vehicular flow on the parameters of signalization as well as the system size and the car density. We investigate various types of the spatial distribution of the vehicular density, and show existence of a shock profile...
March 2017: Physical Review. E
https://www.readbyqxmd.com/read/28413507/anaphylactic-shock-caused-by-haemocoagulase-injection-in-china
#10
Haiwei Yang, Liming Yang, De-Wang Wang, Ji-Fu Wei, Shaoheng He
Haemocoagulase injection is a mixture of purified enzymes isolated from the venom of Bothrops atrox, which is used for the prevention and treatment of haemorrhage. It is a relatively safe pharmacological agent that does not require a skin test prior to administration. However, following a literature search, 14 reported cases of anaphylactic shock caused by haemocoagulase injection were identified, including one lethal case in China. Using SDS-PAGE and protein identification, four primary components in haemocoagulase injection were characterized, including one metalloproteinase, which may be a thromboplastin-like enzyme, and two serine proteinases, which may be thrombin-like enzymes...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28413267/vasopressors-do-they-have-any-role-in-hemorrhagic-shock
#11
REVIEW
Babita Gupta, Neha Garg, Rashmi Ramachandran
The priority in the management of patients with traumatic hemorrhagic shock is to control the bleeding with simultaneous volume resuscitation to maintain adequate tissue perfusion. Fluid replacement remains the mainstay of initial resuscitation in hemorrhagic shock. Traditionally, vasopressors are contraindicated in the early management of hemorrhagic shock due to their deleterious consequences, although vasopressors may have a role in resuscitation when vasoplegic shock ensues and blood pressure cannot be maintained by fluids alone...
January 2017: Journal of Anaesthesiology, Clinical Pharmacology
https://www.readbyqxmd.com/read/28413218/understanding-how-low-socioeconomic-status-households-cope-with-health-shocks-an-analysis-of-multi-sector-linked-data
#12
Tammy Leonard, Amy E Hughes, Sandi L Pruitt
Low-socioeconomic status (SES) households have little income or wealth to buffer against the negative impacts of an adverse health event (health shock) among adult household members. However, these households may employ a variety of other coping strategies such as receiving help from family, friends, and social services. Administrative data from a non-profit food distribution center, electronic medical record (EMR) data from a safety-net healthcare system, and publicly available residential appraisal data were linked to provide insight into these coping strategies...
January 2017: Annals of the American Academy of Political and Social Science
https://www.readbyqxmd.com/read/28413074/long-term-outcomes-of-extracorporeal-membrane-oxygenation-support-for-postcardiotomy-shock
#13
Shao-Wei Chen, Feng-Chun Tsai, Yu-Sheng Lin, Chih-Hsiang Chang, Dong-Yi Chen, An-Hsun Chou, Tien-Hsing Chen
OBJECTIVE: Extracorporeal membrane oxygenation (ECMO) is a widely used technique for treating postcardiotomy cardiogenic shock (PCS); however, no study has compared the long-term outcomes of patients who receive ECMO support for PCS with those of the general population post cardiac surgery. METHODS: A total of 1141 patients who received ECMO after cardiac surgery between 2000 and 2011 were identified by using the Taiwan National Health Insurance Research Database...
March 11, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28412478/mitochondrial-and-peroxisomal-lon-proteases-play-opposing-roles-in-reproduction-and-growth-but-co-function-in-the-normal-development-stress-resistance-and-longevity-of-thermomyces-lanuginosus
#14
Xiao Cui, Yi Wei, Xiang-Li Xie, Li-Na Chen, Shi-Hong Zhang
The ATP-dependent Lon enzyme is a highly conserved protease with multiple roles in diverse species such as fungi; however, there are few reports on Lon enzymes in filamentous fungi. Thermomyces lanuginosus, a typical thermophilic fungus, has been widely studied in physiology and cell biology; thus, studies on Thermomyces Lons are important. Two Lons were bioinformatically deduced in T. lanuginosus. Subcellular localization analysis showed that one is present in mitochondria (MLon), while the other is found in peroxisomes (PLon)...
April 12, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28411694/predictors-of-failure-and-complications-of-catheter-directed-interventions-for-pulmonary-embolism
#15
Efthymios D Avgerinos, Adham N Abou Ali, Nathan L Liang, Elizabeth Genovese, Michael J Singh, Michel S Makaroun, Rabih A Chaer
OBJECTIVE: Catheter-directed interventions (CDIs) are increasingly performed for acute pulmonary embolism (PE) as they are presumed to provide similar therapeutic benefits to systemic thrombolysis while decreasing the dose of thrombolytic required and the associated risks. This study aimed to identify factors associated with CDI failure and to describe anticipated complications. METHODS: Consecutive patients who underwent CDI for massive or submassive PE between 2009 and 2015 were identified; outcomes and complications were retrospectively collected...
May 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28409124/hospital-dissemination-of-tst-1-positive-clonal-complex-5-cc5-methicillin-resistant-staphylococcus-aureus
#16
Min Wang, Yi Zheng, Jose R Mediavilla, Liang Chen, Barry N Kreiswirth, Yajun Song, Ruifu Yang, Hong Du
Methicillin-resistant Staphylococcus aureus (MRSA), is one of the most prevalent clinical pathogens isolated from hospital settings, and has increasingly identified in community settings. In China, the SCCmecIII-ST239 strains are disseminated in different geographic regions, accounting for >75% of all MRSA isolates in some national studies. Here we characterized 150 non-duplicate MRSA isolates collected from February 2012 to May 2013 in a tertiary hospital in Suzhou, Eastern China, to explore the molecular epidemiology...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28409042/nephrologists-hate-the-dialysis-catheters-a-systemic-review-of-dialysis-catheter-associated-infective-endocarditis
#17
Kalyana C Janga, Ankur Sinha, Sheldon Greenberg, Kavita Sharma
A 53-year-old Egyptian female with end stage renal disease, one month after start of hemodialysis via an internal jugular catheter, presented with fever and shortness of breath. She developed desquamating vesiculobullous lesions, widespread on her body. She was in profound septic shock and broad spectrum antibiotics were started with appropriate fluid replenishment. An echocardiogram revealed bulky leaflets of the mitral valve with a highly mobile vegetation about 2.3 cm long attached to the anterior leaflet...
2017: Case Reports in Nephrology
https://www.readbyqxmd.com/read/28406853/the-role-of-computed-tomography-in-the-classification-of-pediatric-pelvic-fractures-revisited
#18
Bent Ma, Hennrikus Wl, Latorre Je, Armstrong Dg, Shaw B, Jones Kc, Segal Ls
OBJECTIVES: To determine the need of CT scans in the assessment of pediatric pelvic fractures DESIGN:: Retrospective Chart Review SETTING:: Level-1 Pediatric Trauma CenterPatients/Participants: 30 pediatric trauma patients with pelvic fractures who have obtained both a radiograph and CT Scan MAIN OUTCOME MEASUREMENTS:: Fleiss Kappa coefficient to compare inter-reliability. RESULTS: The average age of the patients was 7 years (range 1-13 years). 17 were male and 13 were female...
April 10, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28405383/a-new-apparatus-to-induce-lysis-of-planktonic-microbial-cells-by-shock-compression-cavitation-and-spray
#19
A Schiffer, M N Gardner, R H Lynn, V L Tagarielli
Experiments were conducted on an aqueous growth medium containing cultures of Escherichia coli (E. coli) XL1-Blue, to investigate, in a single experiment, the effect of two types of dynamic mechanical loading on cellular integrity. A bespoke shock tube was used to subject separate portions of a planktonic bacterial culture to two different loading sequences: (i) shock compression followed by cavitation, and (ii) shock compression followed by spray. The apparatus allows the generation of an adjustable loading shock wave of magnitude up to 300 MPa in a sterile laboratory environment...
March 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28403119/combined-measurement-of-soluble-st2-and-amino-terminal-pro-b-type-natriuretic-peptide-provides-early-assessment-of-severity-in-cardiogenic-shock-complicating-acute-coronary-syndrome
#20
Heli Tolppanen, Mercedes Rivas-Lasarte, Johan Lassus, Malha Sadoune, Etienne Gayat, Kari Pulkki, Mattia Arrigo, Evguenia Krastinova, Alessandro Sionis, John Parissis, Jindrich Spinar, James Januzzi, Veli-Pekka Harjola, Alexandre Mebazaa
OBJECTIVES: Mortality in cardiogenic shock complicating acute coronary syndrome is high, and objective risk stratification is needed for rational use of advanced therapies such as mechanical circulatory support. Traditionally, clinical variables have been used to judge risk in cardiogenic shock. The aim of this study was to assess the added value of serial measurement of soluble ST2 and amino-terminal pro-B-type natriuretic peptide to clinical parameters for risk stratification in cardiogenic shock...
April 11, 2017: Critical Care Medicine
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