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https://www.readbyqxmd.com/read/27894882/comparing-biomarker-responses-during-thermal-acclimation-a-lethal-vs-non-lethal-approach-in-a-tropical-reef-clownfish
#1
Carolina Madeira, Diana Madeira, Mário S Diniz, Henrique N Cabral, Catarina Vinagre
Knowledge of thermal stress biology for most tropical fish species in reef ecosystems under climate change is still quite limited. Thus, the objective of this study was to measure the time-course changes of thermal stress biomarkers in the commercially exploited coral reef fish Amphiprion ocellaris, during a laboratory simulated event of increased temperature. Heat shock protein 70kDa (Hsp70) and total ubiquitin (Ub) were determined in the muscle (lethal method) and in the fin (non-lethal alternative method) under two temperature treatments (control - 26°C and elevated temperature - 30°C) throughout one month with weekly samplings...
November 25, 2016: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/27829710/early-norepinephrine-decreases-fluid-and-ventilatory-requirements-in-pediatric-vasodilatory-septic-shock
#2
Suchitra Ranjit, Rajeswari Natraj, Sathish Kumar Kandath, Niranjan Kissoon, Balasubramaniam Ramakrishnan, Paul E Marik
AIMS: We previously reported that vasodilatation was common in pediatric septic shock, regardless of whether they were warm or cold, providing a rationale for early norepinephrine (NE) to increase venous return (VR) and arterial tone. Our primary aim was to evaluate the effect of smaller fluid bolus plus early-NE versus the American College of Critical Care Medicine (ACCM) approach to more liberal fluid boluses and vasoactive-inotropic agents on fluid balance, shock resolution, ventilator support and mortality in children with septic shock...
October 2016: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/27627404/dissociation-along-the-principal-hugoniot-of-the-laser-m%C3%A3-gajoule-ablator-material
#3
P Colin-Lalu, V Recoules, G Salin, T Plisson, E Brambrink, T Vinci, R Bolis, G Huser
Glow discharge polymer hydrocarbon (GDP-CH) is used as the ablator material in inertial confinement fusion (ICF) capsules for the Laser Mégajoule and National Ignition Facility. Due to its fabrication process, GDP-CH chemical composition and structure differ from commercially available plastics and detailed knowledge of its properties in the warm dense matter regime is needed to achieve accurate design of ICF capsules. First-principles ab initio simulations of the GDP-CH principal Hugoniot up to 8 Mbar were performed using the quantum molecular dynamics (QMD) code abinit and showed that atomic bond dissociation has an effect on the compressibility...
August 2016: Physical Review. E
https://www.readbyqxmd.com/read/27606618/translation-regulation-and-rna-granule-formation-after-heat-shock-of-procyclic-form-trypanosoma-brucei-many-heat-induced-mrnas-are-also-increased-during-differentiation-to-mammalian-infective-forms
#4
Igor Minia, Clementine Merce, Monica Terrao, Christine Clayton
African trypanosome procyclic forms multiply in the midgut of tsetse flies, and are routinely cultured at 27°C. Heat shocks of 37°C and above result in general inhibition of translation, and severe heat shock (41°C) results in sequestration of mRNA in granules. The mRNAs that are bound by the zinc-finger protein ZC3H11, including those encoding refolding chaperones, escape heat-induced translation inhibition. At 27°C, ZC3H11 mRNA is predominantly present as an untranslated cytosolic messenger ribonucleoprotein particle, but after heat shocks of 37°C-41°C, the ZC3H11 mRNA moves into the polysomal fraction...
September 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27595556/anaerobic-metabolism-and-thermal-tolerance-the-importance-of-opine-pathways-on-survival-of-a-gastropod-after-cardiac-dysfunction
#5
Guodong Han, Shu Zhang, Yunwei Dong
Organisms on the rocky shore frequently suffer from high temperature, which consequently causes disability of cardiac function and retards the cellular oxygen delivery. However, some gastropods can survive in several Celsius degrees higher than their Arrhenius break temperature of cardiac function (ABT), indicating the importance of anaerobic metabolism for their thermal tolerance. We measured the global molecular responses to heat stress in limpet Cellana toreuma using 454 GS-FLX to investigate the variations of genes involving in anaerobic metabolism at high temperature...
September 5, 2016: Integrative Zoology
https://www.readbyqxmd.com/read/27591318/comparative-physiological-biochemical-and-molecular-thermal-stress-response-profiles-for-two-unionid-freshwater-mussel-species
#6
Samantha L Payton, Paul D Johnson, Matthew J Jenny
Freshwater mussels, aquatic keystone species, are in global decline. Long life spans, sedentary lifestyles, and unique reproductive strategies involving obligate parasitic stages make unionid freshwater mussels particularly sensitive to environmental perturbations resulting from global climate change. A greater understanding of the mechanisms by which closely related species differ in their response to thermal challenge is critical for successful conservation and management practices. As such, both an acute heat shock and a chronic warming simulation were conducted in order to evaluate responses between hypothesized thermally tolerant (Villosa lienosa) and thermally sensitive (Villosa nebulosa) freshwater mussels in response to predicted thermal warming...
November 15, 2016: Journal of Experimental Biology
https://www.readbyqxmd.com/read/27586989/-the-452th-case-rash-hypotension-abdominal-pain-and-headache
#7
S N Bian, H H Yang, Q Wang, D Xu, Y Zhao
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized with multiple organ involvements. Acute acalculous cholecystitis(AAC) is an extremely rare manifestation of digestive system involvement in SLE. We reported a case of 32-year-old woman who complained skin rashes for two weeks and stomachache and oliguria for one day. She had rashes at onset, and developed fever, stomachache, hypotension and headache. Physical examination at admission indicated blood pressure 76/47 mmHg(1 mmHg=0.133 kPa), heart rate 107 beats/min, warm acra...
September 1, 2016: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
https://www.readbyqxmd.com/read/27561907/use-of-methylene-blue-to-treat-hypovolemic-shock-followed-by-ischemia-reperfusion-injury-in-the-postoperative-orthotopic-liver-transplant-patient-a-case-report
#8
Kelvin Henrique Vilalva, Fabrícia Teixeira Mumic, Marina Rodrigues Garcia Silveira, Enio David Mente, Paulo Roberto Barbosa Evora, Orlando Castro E Silva
A 57-year-old female patient received elective liver transplant due to nonalcoholic steatohepatitis complicated by hepatocellular carcinoma. Her preoperative Model for End-Stage Liver Disease score was 11. The total transplant ischemic time was 10 hours and 35 minutes, and the warm ischemic time was 35 minutes. Even with aggressive fluid overload and use of high concentrations of vasoactive amines, the patient developed possible primary graft dysfunction with poor response to fluids and vasopressor support, suggesting vasoplegic syndrome...
August 24, 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27513861/hdaci-valproic-acid-vpa-and-suberoylanilide-hydroxamic-acid-saha-delay-but-fail-to-protect-against-warm-hepatic-ischemia-reperfusion-injury
#9
Dietrich A Ruess, Moriz Probst, Goran Marjanovic, Uwe A Wittel, Ulrich T Hopt, Tobias Keck, Dirk Bausch
BACKGROUND: Histone deacetylases (HDAC) catalyze N-terminal deacetylation of lysine-residues on histones and multiple nuclear and cytoplasmic proteins. In various animal models, such as trauma/hemorrhagic shock, ischemic stroke or myocardial infarction, HDAC inhibitor (HDACi) application is cyto- and organoprotective and promotes survival. HDACi reduce stress signaling, cell death and inflammation. Hepatic ischemia-reperfusion (I/R) injury during major liver resection or transplantation increases morbidity and mortality...
2016: PloS One
https://www.readbyqxmd.com/read/27491440/functional-evaluation-of-heat-shock-proteins-70-hsp70-hsc70-on-rhodnius-prolixus-hemiptera-reduviidae-physiological-responses-associated-with-feeding-and-starvation
#10
Rafaela M M Paim, Ricardo N Araujo, Miguel Leis, Mauricio R V Sant'anna, Nelder F Gontijo, Claudio R Lazzari, Marcos H Pereira
Blood-sucking vectors must overcome thermal stress caused by intake of proportionally large amounts of warm blood from their hosts. In response to this, Heat Shock Proteins (HSPs) such as the widely studied HSP70 family (the inducible HSP70 and the cognate form HSC70, known for their role in preserving essential cellular functions) are rapidly up-regulated in their tissues. The triatomine Rhodnius prolixus is an important vector of Trypanosoma cruzi, the causative pathogen of Chagas' disease, and is also a model organism for studying insect biology and physiology...
October 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27470130/short-term-treadmill-exercise-in-a-cold-environment-does-not-induce-adrenal-hsp72-and-hsp25-expression
#11
Senay Akin, Hisashi Naito, Yuji Ogura, Noriko Ichinoseki-Sekine, Mitsutoshi Kurosaka, Ryo Kakigi, Haydar A Demirel
Heat shock proteins (Hsps) have a critical role in maintaining cellular homeostasis and in protecting cells from a range of acute and chronic stressful conditions. Treadmill running exercise results in increased Hsp72 and Hsp25 levels in various tissues and heat production during exercise has been shown to be the main factor for the increased levels of Hsp72 in myocardium. Since the adrenal gland plays a vital role in general response to stress, regulation of Hsps in adrenal glands following stressful events seems to be critical for controlling the whole-body stress response appropriately...
July 28, 2016: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/27460544/the-regulation-of-thermal-stress-induced-apoptosis-in-corals-reveals-high-similarities-in-gene-expression-and-function-to-higher-animals
#12
Hagit Kvitt, Hanna Rosenfeld, Dan Tchernov
Recent studies suggest that controlled apoptotic response provides an essential mechanism, enabling corals to respond to global warming and ocean acidification. However, the molecules involved and their functions are still unclear. To better characterize the apoptotic response in basal metazoans, we studied the expression profiles of selected genes that encode for putative pro- and anti-apoptotic mediators in the coral Stylophora pistillata under thermal stress and bleaching conditions. Upon thermal stress, as attested by the elevation of the heat-shock protein gene HSP70's mRNA levels, the expression of all studied genes, including caspase, Bcl-2, Bax, APAF-1 and BI-1, peaked at 6-24 h of thermal stress (hts) and declined at 72 hts...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27439426/temperature-desynchronizes-sugar-and-organic-acid-metabolism-in-ripening-grapevine-fruits-and-remodels-their-transcriptome
#13
Markus Rienth, Laurent Torregrosa, Gautier Sarah, Morgane Ardisson, Jean-Marc Brillouet, Charles Romieu
BACKGROUND: Fruit composition at harvest is strongly dependent on the temperature during the grapevine developmental cycle. This raises serious concerns regarding the sustainability of viticulture and the socio-economic repercussions of global warming for many regions where the most heat-tolerant varieties are already cultivated. Despite recent progress, the direct and indirect effects of temperature on fruit development are far from being understood. Experimental limitations such as fluctuating environmental conditions, intra-cluster heterogeneity and the annual reproductive cycle introduce unquantifiable biases for gene expression and physiological studies with grapevine...
2016: BMC Plant Biology
https://www.readbyqxmd.com/read/27357258/cold-acclimation-wholly-reorganizes-the-drosophila-melanogaster-transcriptome-and-metabolome
#14
Heath A MacMillan, Jose M Knee, Alice B Dennis, Hiroko Udaka, Katie E Marshall, Thomas J S Merritt, Brent J Sinclair
Cold tolerance is a key determinant of insect distribution and abundance, and thermal acclimation can strongly influence organismal stress tolerance phenotypes, particularly in small ectotherms like Drosophila. However, there is limited understanding of the molecular and biochemical mechanisms that confer such impressive plasticity. Here, we use high-throughput mRNA sequencing (RNA-seq) and liquid chromatography - mass spectrometry (LC-MS) to compare the transcriptomes and metabolomes of D. melanogaster acclimated as adults to warm (rearing) (21...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27320381/transcriptional-regulation-of-heat-shock-proteins-and-ascorbate-peroxidase-by-cthsfa2b-from-african-bermudagrass-conferring-heat-tolerance-in-arabidopsis
#15
Xiuyun Wang, Wanlu Huang, Zhimin Yang, Jun Liu, Bingru Huang
Heat stress transcription factor A2s (HsfA2s) are key regulators in plant response to high temperature. Our objectives were to isolate an HsfA2 gene (CtHsfA2b) from a warm-season grass species, African bermudagrass (Cynodon transvaalensis Burtt-Davy), and to determine the physiological functions and transcriptional regulation of HsfA2 for improving heat tolerance. Gene expression analysis revealed that CtHsfA2b was heat-inducible and exhibited rapid response to increasing temperature. Ectopic expression of CtHsfA2b improved heat tolerance in Arabidopsis and restored heat-sensitive defects of Arabidopsis hsfa2 mutant, which was demonstrated by higher survival rate and photosynthetic parameters, and lower electrolyte leakage in transgenic plants compared to the WT or hsfa2 mutant...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27312151/biodiversity-in-marine-invertebrate-responses-to-acute-warming-revealed-by-a-comparative-multi-omics-approach
#16
Melody S Clark, Ulf Sommer, Jaspreet K Sihra, Michael A S Thorne, Simon A Morley, Michelle King, Mark R Viant, Lloyd S Peck
Understanding species' responses to environmental change underpins our abilities to make predictions on future biodiversity under any range of scenarios. In spite of the huge biodiversity in most ecosystems, a model species approach is often taken in environmental studies. To date, we still do not know how many species we need to study to input into models and inform on ecosystem-level responses to change. In this study, we tested current paradigms on factors setting thermal limits by investigating the acute warming response of six Antarctic marine invertebrates: a crustacean Paraceradocus miersi, a brachiopod Liothyrella uva, two bivalve molluscs, Laternula elliptica, Aequiyoldia eightsii, a gastropod mollusc Marseniopsis mollis and an echinoderm Cucumaria georgiana...
June 17, 2016: Global Change Biology
https://www.readbyqxmd.com/read/27300976/modeling-asymmetric-cavity-collapse-with-plasma-equations-of-state
#17
Brett Tully, Nicholas Hawker, Yiannis Ventikos
We explore the effect that equation of state (EOS) thermodynamics has on shock-driven cavity-collapse processes. We account for full, multidimensional, unsteady hydrodynamics and incorporate a range of relevant EOSs (polytropic, QEOS-type, and SESAME). In doing so, we show that simplified analytic EOSs, like ideal gas, capture certain critical parameters of the collapse such as velocity of the main transverse jet and pressure at jet strike, while also providing a good representation of overall trends. However, more sophisticated EOSs yield different and more relevant estimates of temperature and density, especially for higher incident shock strengths...
May 2016: Physical Review. E
https://www.readbyqxmd.com/read/27293764/small-pelagics-in-a-changing-ocean-biological-responses-of-sardine-early-stages-to-warming
#18
Filipa Faleiro, Marta Pimentel, Maria Rita Pegado, Regina Bispo, Ana Rita Lopes, Mário S Diniz, Rui Rosa
Small pelagic fishes are known to respond rapidly to changes in ocean climate. In this study, we evaluate the effects of future environmental warming (+2°C) during the early ontogeny of the European sardine, Sardina pilchardus. Warming reduced the survival of 30-day-old larvae by half. Length at hatching increased with temperature as expected, but no significant effect was observed on the length and growth at 30 days post-hatching. Warming did not significantly affect the thermal tolerance of sardine larvae, even though the mean lethal temperature increased by 1°C...
2016: Conservation Physiology
https://www.readbyqxmd.com/read/27246145/probing-local-and-electronic-structure-in-warm-dense-matter-single-pulse-synchrotron-x-ray-absorption-spectroscopy-on-shocked-fe
#19
Raffaella Torchio, Florent Occelli, Olivier Mathon, Arnaud Sollier, Emilien Lescoute, Laurent Videau, Tommaso Vinci, Alessandra Benuzzi-Mounaix, Jon Headspith, William Helsby, Simon Bland, Daniel Eakins, David Chapman, Sakura Pascarelli, Paul Loubeyre
Understanding Warm Dense Matter (WDM), the state of planetary interiors, is a new frontier in scientific research. There exists very little experimental data probing WDM states at the atomic level to test current models and those performed up to now are limited in quality. Here, we report a proof-of-principle experiment that makes microscopic investigations of materials under dynamic compression easily accessible to users and with data quality close to that achievable at ambient. Using a single 100 ps synchrotron x-ray pulse, we have measured, by K-edge absorption spectroscopy, ns-lived equilibrium states of WDM Fe...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27193989/should-warm-fresh-whole-blood-be-the-first-choice-in-acute-massive-hemorrhage-in-emergency-conditions
#20
Pınar Kendigelen, Zeynep Kamalak, Deniz Abat
Early management of rapid massive hemorrhage requires early administration of blood products and rapid surgical control of bleeding. Professionals in peripheral hospitals with limited resources often work under conditions similar to those in the military. Described in the present report are 3 cases in which warm fresh whole blood (WFWB) was used in patients with massive bleeding who presented to a peripheral hospital that had no blood products suitable for emergency conditions. Described first is the case of a 16-year-old female patient who underwent emergency cesarean section...
March 2016: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
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