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https://www.readbyqxmd.com/read/29777513/a-novel-malic-enzyme-gene-mime2-from-mortierella-isabellina-m6-22-contributes-to-lipid-accumulation
#1
Shan Li, Lingyan Li, Xiangfeng Xiong, Xiuling Ji, Yunlin Wei, Lianbing Lin, Qi Zhang
OBJECTIVE: This study was aimed at cloning and characterizing a novel malic enzyme (ME) gene of Mortierella isabellina M6-22 and identifying its relation with lipid accumulation. METHODS: Mime2 was cloned from strain M6-22. Plasmid pET32aMIME2 was constructed to express ME of MIME2 in Escherichia coli BL21. After purification, the optimal pH and temperature of MIME2, as well as Km and Vmax for NADP+ were determined. The effects of EDTA or metal ions (Mn2+ , Mg2+ , Co2+ , Cu2+ , Ca2+ , or Zn2+ ) on the enzymatic activity of MIME2 were evaluated...
May 18, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29777512/discovery-of-an-acidic-thermostable-and-highly-nadp-dependent-formate-dehydrogenase-from-lactobacillus-buchneri-nrrl-b-30929
#2
Saadet Alpdağtaş, Sevil Yücel, Handan Açelya Kapkaç, Siqing Liu, Barış Binay
OBJECTIVES: To identify a robust NADP+ dependent formate dehydrogenase from Lactobacillus buchneri NRRL B-30929 (LbFDH) with unique biochemical properties. RESULTS: A new NADP+ dependent formate dehydrogenase gene (fdh) was cloned from genomic DNA of L. buchneri NRRL B-30929. The recombinant construct was expressed in Escherichia coli BL21(DE3) with 6 × histidine at the C-terminus and the purified protein obtained as a single band of approx. 44 kDa on SDS-PAGE and 90 kDa on native-PAGE...
May 18, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29775711/co-expression-purification-and-characterization-of-the-lipase-and-foldase-of-burkholderia-contaminans-lteb11
#3
Robson Carlos Alnoch, Adriano Alves Stefanello, Viviane de Paula Martini, Jeferson Luiz Richter, Cesar Mateo, Emanuel Maltempi de Souza, David Alexander Mitchell, Marcelo Muller-Santos, Nadia Krieger
Genes encoding lipase LipBC (lipA) and foldase LifBC (lipB) were identified in the genome of Burkholderia contaminans LTEB11. Analysis of the predicted amino acid sequence of lipA showed its high identity with lipases from Pseudomonas luteola (91%), Burkholderia cepacia (96%) and Burkholderia lata (97%), and classified LipBC lipase in the lipase subfamily I.2. The genes lipA and lipB were amplified and cloned into expression vectors pET28a(+) and pT7-7, respectively. His-tagged LipBC and native LifBC were co-expressed in Escherichia coli and purified...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775480/titan-cells-formation-in-cryptococcus-neoformans-is-finely-tuned-by-environmental-conditions-and-modulated-by-positive-and-negative-genetic-regulators
#4
Benjamin Hommel, Liliane Mukaremera, Radames J B Cordero, Carolina Coelho, Christopher A Desjardins, Aude Sturny-Leclère, Guilhem Janbon, John R Perfect, James A Fraser, Arturo Casadevall, Christina A Cuomo, Françoise Dromer, Kirsten Nielsen, Alexandre Alanio
The pathogenic fungus Cryptococcus neoformans exhibits morphological changes in cell size during lung infection, producing both typical size 5 to 7 μm cells and large titan cells (> 10 μm and up to 100 μm). We found and optimized in vitro conditions that produce titan cells in order to identify the ancestry of titan cells, the environmental determinants, and the key gene regulators of titan cell formation. Titan cells generated in vitro harbor the main characteristics of titan cells produced in vivo including their large cell size (>10 μm), polyploidy with a single nucleus, large vacuole, dense capsule, and thick cell wall...
May 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29775477/cryptococcus-neoformans-can-form-titan-like-cells-in-vitro-in-response-to-multiple-signals
#5
Nuria Trevijano-Contador, Haroldo Cesar de Oliveira, Rocío García-Rodas, Suélen Andreia Rossi, Irene Llorente, Ángel Zaballos, Guilhem Janbon, Joaquín Ariño, Óscar Zaragoza
Cryptococcus neoformans is an encapsulated pathogenic yeast that can change the size of the cells during infection. In particular, this process can occur by enlarging the size of the capsule without modifying the size of the cell body, or by increasing the diameter of the cell body, which is normally accompanied by an increase of the capsule too. This last process leads to the formation of cells of an abnormal enlarged size denominated titan cells. Previous works characterized titan cell formation during pulmonary infection but research on this topic has been hampered due to the difficulty to obtain them in vitro...
May 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29775177/novosphingobium-arvoryzae-sp-nov-isolated-from-a-flooded-rice-field
#6
Shih-Yi Sheu, Cheng-Wen Huang, Jhen-Ci Chen, Zih-Han Chen, Wen-Ming Chen
A bacterial strain, designated Jyi-02T , was isolated from a flooded rice field in Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain Jyi-02T were aerobic, Gram-stain-negative, rod-shaped, non-motile and formed yellowish orange coloured colonies. Growth occurred at 10-40 °C (optimum, 20 °C) and pH 5.0-9.0 (optimum, pH 7.0) and in the presence of 0-1.0 % NaCl (optimum, 0 %, w/v). The major fatty acids (>10 %) of strain Jyi-02T were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C14 : 0 2-OH...
May 18, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29775176/chitinophaga-varians-sp-nov-isolated-from-forest-soil
#7
Ying-Ying Lv, Xian-Jiao Zhang, An-Zhang Li, Wei-Ling Zou, Guang-da Feng, Hong-Hui Zhu
A Gram-stain-negative, aerobic, non-motile bacterium, designated strain 10-7W-9003T , was isolated from the forest soil of Limushan National Forest Park, south-east China (19° 10' 42″ N, 109° 44' 45″ E). Strain 10-7W-9003T showed a shape change during the course of culture from long filamentous cells (5-10×0.4-0.5 µm) at 5-36 h, to rod shaped (1.0-1.5×0.5-0.7 µm) with inoculation after 2 days. It grew optimally at 28-30 °C and pH 6.5-7.5. On the basis of 16S rRNA gene sequence analysis, it belongs to the genus Chitinophaga and is most closely related to Chitinophaga eiseniae KACC 13774T and Chitinophaga qingshengii JCM 30026T , with 16S rRNA gene sequences similarities of 98...
May 18, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29774508/the-bacterial-and-archaeal-community-structures-and-methanogenic-potential-of-the-cecal-microbiota-of-goats-fed-with-hay-and-high-grain-diets
#8
Wei Jin, Yin Li, Yanfen Cheng, Shengyong Mao, Weiyun Zhu
The cecum plays an important role in the feed fermentation of ruminants. However, information is very limited regarding the cecal microbiota and their methane production. In the present study, the cecal content from twelve local Chinese goats, fed with either a hay diet (0% grain) or a high-grain diet (71.5% grain), were used to investigate the bacterial and archaeal community and their methanogenic potential. Microbial community analysis was determined using high-throughput sequencing of 16S rRNA genes and real-time PCR, and the methanogenesis potential was assessed by in vitro fermentation with ground corn or hay as substrates...
May 17, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29771319/distribution-and-function-of-carbamate-hydrolase-genes-ceha-and-mcd-in-soils-the-distinct-role-of-soil-ph
#9
Constantina Rousidou, Dionysis Karaiskos, Despoina Myti, Evangelos Karanasios, Panagiotis A Karas, Maria Tourna, Emmanuel A Tzortzakakis, Dimitrios G Karpouzas
No abstract text is available yet for this article.
June 1, 2018: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29770864/agrobacterium-mediated-transformation-of-yeast-and-fungi
#10
Paul J J Hooykaas, G Paul H van Heusden, Xiaolei Niu, M Reza Roushan, Jalal Soltani, Xiaorong Zhang, Bert J van der Zaal
Two decades ago, it was discovered that the well-known plant vector Agrobacterium tumefaciens can also transform yeasts and fungi when these microorganisms are co-cultivated on a solid substrate in the presence of a phenolic inducer such as acetosyringone. It is important that the medium has a low pH (5-6) and that the temperature is kept at room temperature (20-25 °C) during co-cultivation. Nowadays, Agrobacterium-mediated transformation (AMT) is the method of choice for the transformation of many fungal species; as the method is simple, the transformation efficiencies are much higher than with other methods, and AMT leads to single-copy integration much more frequently than do other methods...
May 17, 2018: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/29767618/hymenobacter-pedocola-sp-nov-a-novel-bacterium-isolated-from-soil
#11
Soo-Jeong Lim, Leonid N Ten, Byung-Oh Kim, In-Kyu Kang, Hee-Young Jung
Strain S12-2-1T was isolated from a soil sample collected in the Gyeongsangnam-do province of the Republic of Korea. The isolate is a Gram-stain-negative, aerobic, short, rod-shaped bacterium, and its colonies are red to pink in colour. Analysis of the 16S rRNA gene identified strain S12-2-1T as a member of the genus Hymenobacter in the family Cytophagaceae, with high levels of 16S rRNA gene sequence similarity to Hymenobacter arizonensis OR362-8T (97.7 %), Hymenobacter sedentarius DG5BT (97.4 %) and Hymenobacter humi DG31AT (97...
May 16, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29767617/mangrovicoccus-ximenensis-gen-nov-sp-nov-isolated-from-mangrove-forest-sediment
#12
Zhiliang Yu, Yang Cao, Guoqiang Zhou, Jianhua Yin, Juanping Qiu
A Gram-strain-negative, coccoid bacterium, lacking bacteriochlorophyll, designated strain T1lg56T , was isolated from a sediment sample collected from Ximen island mangrove forest, Zhejiang province, China. Cells were halotolerant, and catalase- and oxidase-positive. Growth was observed at 18-42 °C (optimum, 35 °C), at pH 6.0-9.5 (optimum, pH 6.5) and in the presence of 0-15 % (w/v) NaCl (optimum, 2-5 %). The major cellular fatty acids were C18 : 1ω7c and C16 : 0. The polar lipid profile of strain T1lg56T consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmonomethylethanolamine, two unidentified phospholipids and five unidentified lipids...
May 16, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29767296/evaluating-the-antimicrobial-apoptotic-and-cancer-cell-gene-delivery-properties-of-protein-capped-gold-nanoparticles-synthesized-from-the-edible-mycorrhizal-fungus-tricholoma-crassum
#13
Arpita Basu, Sarmishtha Ray, Supriyo Chowdhury, Arnab Sarkar, Deba Prasad Mandal, Shamee Bhattacharjee, Surekha Kundu
Biosynthesis of gold nanoparticles of distinct geometric shapes with highly functional protein coats without additional capping steps is rarely reported. This study describes green synthesis of protein-coated gold nanoparticles for the first time from the edible, mycorrhizal fungus Tricholoma crassum (Berk.) Sacc. The nanoparticles were of the size range 5-25 nm and of different shapes. Spectroscopic analysis showed red shift of the absorption maxima with longer reaction period during production and blue shift with increase in pH...
May 16, 2018: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29765908/the-subgingival-microbiome-of-periodontal-pockets-with-different-probing-depths-in-chronic-and-aggressive-periodontitis-a-pilot-study
#14
Meng Shi, Yiping Wei, Wenjie Hu, Yong Nie, Xiaolei Wu, Ruifang Lu
Periodontitis is a kind of infectious disease initiated by colonization of subgingival periodontal pathogens, which cause destruction of tooth-supporting tissues, and is a predominant threat to oral health as the most common cause of loss of teeth. The aim of this pilot study was to characterize the subgingival bacterial biodiversity of periodontal pockets with different probing depths in patients with different forms of periodontitis. Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive periodontitis (AgP) and three periodontally healthy subjects (PH)...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29762617/anticancer-potential-against-cervix-cancer-hela-cell-line-of-probiotic-lactobacillus-casei-and-lactobacillus-paracasei-strains-isolated-from-human-breast-milk
#15
Muhammad Shahid Riaz Rajoka, Haobin Zhao, Yao Lu, Ziyang Lian, Na Li, Nazim Hussain, Dongyan Shao, Mingliang Jin, Qi Li, Junling Shi
Lactic acid bacteria have been categorized as probiotics and play a crucial role in human health by stimulating the supply of nutrients, shaping the immune system, and preventing the colonization of pathogenic microbes. This study investigated the mechanisms for the action of three potential probiotic Lactobacillus strains: Lactobacillus casei SR1, Lactobacillus casei SR2, and Lactobacillus paracasei SR4 isolated from human breast milk. These Lactobacillus strains were identified via 16S DNA sequencing and characterized via biochemical assays including acid resistance, bile resistance, antioxidant activity, and antibiotic susceptibility...
May 15, 2018: Food & Function
https://www.readbyqxmd.com/read/29762105/kandeliimicrobium-roseum-gen-nov-sp-nov-a-new-member-of-the-family-rhodobacteraceae-isolated-from-mangrove-rhizosphere-soil
#16
Kai-Ling Wang, Zhi-Man Song, Cu-Huang Rong, Ling-Yun Hao, Qi-Liang Lai, Shuang-Fei Li, Ying Xu
A Gram-stain-negative, non-flagellated, short rod-shaped bacterium, designated XY-R6T , was isolated from the rhizosphere soil of a mangrove plant, Kandelia candel (L.) Druce, in Mai Po Nature Reserve, Hong Kong. Growth of strain XY-R6T was observed at pH 5.0-9.5 (optimum 6.5-8.0), between 8 and 42 °C (optimum 28-34 °C), and in the presence of 0-9.5 % (w/v) NaCl (optimum 1-4 %). The predominant isoprenoid quinone was ubiquinone-10. The major fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c) (55...
May 15, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29761789/ph-and-redox-responsive-nanoparticles-composed-of-charge-reversible-pullulan-based-shells-and-disulfide-containing-poly-%C3%AE-amino-ester-cores-for-co-delivery-of-gene-and-chemotherapeutic-agent
#17
Sipei Zhang, Dan Wang, Yating Li, Ling Li, Hongli Chen, Qingqing Xiong, Yuan-Yuan Liu, Yingsong Wang
A novel pH- and redox-responsive nanoparticle system was designed based on charge-reversible pullulan derivative (CAPL) and disulfide-containing poly(β-amino ester) (ssPBAE) for the co-delivery of gene and chemotherapeutic agent targeting hepatoma. The end-alkene groups of ssPBAE were reacted with diethylenetriamine to form amino-modified ssPBAE (NH-ssPBAE). Methotrexate (MTX), a chemotherapy agent, was then conjugated to NH-ssPBAE via amide bond to obtain a polymeric prodrug ssPBAE-MTX. ssPBAE-MTX exhibited a good capability for condensing genes including plasmid DNA (pDNA) and tetramethyl rhodamine-labeled DNA (TAMRA-DNA), and almost completely condensed pDNA at the weight ratio of 5/1 to form spherical nanocomplexes with an uniform size...
May 15, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29761247/beneficial-effects-of-fenofibrate-in-pulmonary-hypertension-in-rats
#18
Palak Galhotra, Pankaj Prabhakar, Himanshu Meghwani, Soheb A Mohammed, Sanjay Kumar Banerjee, Sandeep Seth, Milind P Hote, K H Reeta, Ruma Ray, Subir Kumar Maulik
Pulmonary hypertension (PH) is a morbid complication of cardiopulmonary as well as several systemic diseases in humans. It is rapidly progressive and fatal if left untreated. In the present study, we investigated the effect of PPARα agonist fenofibrate (FF) on monocrotaline (MCT)-induced PH in rats. FF, because of its pleiotropic property, could be helpful in reducing inflammation, oxidative stress, and reactive oxygen species. On day 1, MCT (50 mg/kg, s.c.) was given to all the rats in MCT, sildenafil, and FF group except normal control rats...
May 14, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29760688/dissecting-the-acid-stress-response-of-rhizobium-tropici-ciat-899
#19
Julio Guerrero-Castro, Luis Lozano, Christian Sohlenkamp
Rhizobium tropici CIAT899 is a nodule-forming α-proteobacterium displaying intrinsic resistance to several abiotic stress conditions such as low pH and high temperatures, which are common in tropical environments. It is a good competitor for Phaseolus vulgaris (common bean) nodule occupancy at low pH values, however little is known about the genetic and physiological basis of the tolerance to acidic conditions. To identify genes in R. tropici involved in pH stress response we combined two different approaches: (1) A Tn 5 mutant library of R...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29760185/stability-of-an-aggregation-prone-partially-folded-state-of-human-profilin-1-correlates-with-aggregation-propensity
#20
Edoardo Del Poggetto, Angelo Toto, Chiara Aloise, Francesco Di Piro, Ludovica Gori, Francesco Malatesta, Stefano Gianni, Fabrizio Chiti, Francesco Bemporad
A set of missense mutations in the gene encoding profilin-1 has been linked to the onset of familiar forms of amyotrophic lateral sclerosis (fALS, also known as Lou Gehrig's disease). The pathogenic potential of these mutations is linked to the formation of intracellular inclusions of the mutant proteins and correlates with the mutation-induced destabilization of its native, fully folded state. However, the mechanism by which these mutations promote misfolding and self-assembly is yet unclear. Here, using temperature-jump and stopped-flow kinetic measurements, we show that, during refolding, wild-type profilin-1 transiently populates a partially folded (PF) state endowed with hydrophobic clusters exposed to the solvent and with no detectable secondary structure...
May 14, 2018: Journal of Biological Chemistry
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