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https://www.readbyqxmd.com/read/28925024/complete-characterization-of-new-isolates-of-neptunomonas-phycophila-leads-to-emend-its-description-and-opens-possibilities-of-biotechnological-applications
#1
Ana L Diéguez, Phillip Pichon, Sabela Balboa, Thorolf Magnesen, Jesús L Romalde
Five strains were isolated from gonad of Great scallop (Pecten maximus) broodstock in a Norwegian hatchery. The study of 16S rRNA gene sequences showed that these isolates belong to Neptunomonas phycophila, a bacterium originally isolated from a symbiont of the anemone Aiptasia tagetes from Puerto Rico. The gyrB and rpoB genes sequences confirmed the affiliation of the scallop isolates to this species. Phenotypic characterization was performed and some differences between the Norwegian isolates and the type strain of N...
September 18, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28924033/rgpf-is-required-for-maintenance-of-stress-tolerance-and-virulence-in-streptococcus-mutans
#2
C J Kovacs, R C Faustoferri, R G Quivey
Bacterial cell wall dynamics have been implicated as important determinants of cellular physiology, stress tolerance and virulence. In Streptococcus mutans, the cell wall is composed primarily of a rhamnose-glucose polysaccharide (RGP) linked to the peptidoglycan. Despite extensive studies describing its formation and composition, the potential roles for RGP in S. mutans biology have not been well investigated. The present study characterizes the impact of RGP disruption as a result of deletion of rgpF, the gene encoding a rhamnosyltransferase involved in construction of the core poly-rhamnose backbone of RGP...
September 18, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28923727/role-of-induced-glutathione-s-transferase-from-helicoverpa-armigera-lepidoptera-noctuidae-hagst-8-in-detoxification-of-pesticides
#3
Chaitali P Labade, Abhilash R Jadhav, Mehul Ahire, Smita S Zinjarde, Vaijayanti A Tamhane
The present study deals with glutathione-S-transferase (GST) based detoxification of pesticides in Helicoverpa armigera and its potential application in eliminating pesticides from the environment. Dietary exposure of a pesticide mixture (organophosphates - chlorpyrifos and dichlorvos, pyrethroid - cypermethrin; 2-15ppm each) to H. armigera larvae resulted in a dose dependant up-regulation of GST activity and gene expression. A variant GST from H. armigera (HaGST-8) was isolated from larvae fed with 10ppm pesticide mixture and it was recombinantly expressed in yeast (Pichia pastoris HaGST-8)...
September 15, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28923368/insights-into-the-structural-biology-of-gaucher-disease
#4
REVIEW
Laura Smith, Stephen Mullin, Anthony H V Schapira
Gaucher disease, the most common lysosomal storage disorder, is caused by mutations in the gene encoding the acid-β-glucosidase lysosomal hydrolase enzyme that cleaves glucocerebroside into glucose and ceramide. Reduced enzyme activity and impaired structural stability arise due to >300 known disease-causing mutations. Several of these mutations have also been associated with an increased risk of Parkinson disease (PD). Since the discovery of the acid-β-glucosidase X-ray structure, there have been major advances in our understanding of the structural properties of the protein...
September 15, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28922872/ancient-occasional-host-switching-of-maternally-transmitted-bacterial-symbionts-of-chemosynthetic-vesicomyid-clams
#5
Genki Ozawa, Shigeru Shimamura, Yoshihiro Takaki, Kiyotaka Takishita, Tetsuro Ikuta, James P Barry, Tadashi Maruyama, Katsunori Fujikura, Takao Yoshida
Vesicomyid clams in deep-sea chemosynthetic ecosystems harbor sulfur-oxidizing bacteria in their gill epithelial cells. These symbionts, which are vertically transmitted, are species-specific and thought to have cospeciated with their hosts. However, recent studies indicate incongruent phylogenies between some vesicomyid clams and their symbionts, suggesting that symbionts are horizontally transmitted. To more precisely understand the evolution of vesicomyid clams and their symbionts, we compared the evolution of vesicomyid clams and their symbionts through phylogenetic analyses using multi-gene data sets...
September 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28922793/diurnal-cycling-transcription-factors-of-pineapple-revealed-by-genome-wide-annotation-and-global-transcriptomic-analysis
#6
Anupma Sharma, Ching Man Wai, Ray Ming, Qingyi Yu
Circadian clock provides fitness advantage by coordinating internal metabolic and physiological processes to external cyclic environments. Core clock components exhibit daily rhythmic changes in gene expression, and the majority of them are transcription factors (TFs) and transcription coregulators (TCs). We annotated 1,398 TFs from 67 TF families and 80 TCs from 20 TC families in pineapple, and analyzed their tissue-specific and diurnal expression patterns. Approximately 42% of TFs and 45% of TCs displayed diel rhythmic expression, including 177 TF/TCs cycling only in the nonphotosynthetic leaf tissue, 247 cycling only in the photosynthetic leaf tissue, and 201 cycling in both...
September 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28921896/evaluating-potential-risks-of-food-allergy-and-toxicity-of-soy-leghemoglobin-expressed-in-pichia-pastoris
#7
Yuan Jin, Xiaoyun He, Kwame Andoh-Kumi, Rachel Z Fraser, Mei Lu, Richard E Goodman
SCOPE: The Soybean (Glycine max) leghemoglobin c2 (LegHb) gene was introduced into Pichia pastoris yeast for sustainable production of a heme-carrying protein, for organoleptic use in plant-based meat. The potential allergenicity and toxicity of LegHb and 17 Pichia host-proteins each representing ≥1% of total protein in production batches were evaluated by literature review, bioinformatics sequence comparisons to known allergens or toxins, and in vitro pepsin digestion. METHODS AND RESULTS: Literature searches found no evidence of allergenicity or toxicity for these proteins...
September 18, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28920855/pfma-a-novel-quorum-quenching-n-acylhomoserine-lactone-acylase-from-pseudoalteromonas-flavipulchra
#8
Na Liu, Min Yu, Youbin Zhao, Jingguang Cheng, Ke An, Xiao-Hua Zhang
Many bacteria, such as Proteobacteria, Cyanobacteria and Bacteroidetes, use N-acylhomoserine lactones (AHLs) as quorum-sensing (QS) signal molecules for communication. Enzymatic degradation of AHLs, such as AHL acylase and AHL lactonase, can degrade AHLs (quorum quenching, QQ) to attenuate or disarm the virulence of pathogens. QQ is confirmed to be common in marine bacterial communities. Many genes encoding AHL acylases are found in marine bacteria and metagenomic collections, but only a few of these have been characterized in detail...
September 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28920851/loktanella-acticola-sp-nov-isolated-from-seawater
#9
Sooyeon Park, Su Jung Choi, Sung-Min Won, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated OISW-6T, was isolated from seawater near Oido, a South Korean island, and subjected to a polyphasic taxonomic study. Strain OISW-6T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-6T fell within the clade comprising the type strains of Loktanella species. Strain OISW-6T exhibited 16S rRNA gene sequence similarity values of 97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920850/ramlibacter-alkalitolerans-sp-nov-alkali-tolerant-bacterium-isolated-from-soil-of-ginseng
#10
Do-Hoon Lee, Chang-Jun Cha
A novel bacterial strain, designated CJ661T, was isolated from soil of ginseng in Anseong, South Korea. Cells of strain CJ661T were white-coloured, Gram-staining-negative, non-motile, aerobic and rod-shaped. Strain CJ661T grew optimally at 30 °C and pH 7.0. The analysis of 16S rRNA gene sequence of strain CJ661T showed that it belongs to the genus Ramlibacter within the family Comamonadaceae and was most closely related to Ramlibacter ginsenosidimutans KCTC 22276T (98.1 %), followed by Ramlibacter henchirensis DSM 14656T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920849/flavimarina-flava-sp-nov-isolated-from-salicornia-herbacea
#11
Eui-Sang Cho, In-Tae Cha, Jung-Min Park, Hak-Jong Choi, Jong Hun Lee, Seong Woon Roh, Eun-Ah Cho, Mee-Hyang Kweon, Young-Do Nam, Myung-Ji Seo
A Gram-stain-negative, motile-by-gliding, aerobic, non-spore-forming, rod-shaped and yellow-pigmented bacterium was isolated from Salicornia herbacea in the Yellow Sea and designated as strain MBLN091T. It belonged to the family Flavobacteriaceae. The 16S rRNA gene sequence of this isolated strain was similar to that of Flavimarina pacifica IDSW-73T with 94.8 % similarity, and with 92.3-92.8 % similarities to those of other closely related species of the genus Leeuwenhoekiella. The similarities of the RNA polymerase subunit B gene between this strain and F...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920848/dyella-agri-sp-nov-isolated-from-reclaimed-grassland-soil
#12
Dhiraj Kumar Chaudhary, Jaisoo Kim
A novel strain, DKC-1T, was isolated from reclaimed grassland soil and was characterized taxonomically by a polyphasic approach. Strain DKC-1T was a Gram-staining-negative, light-yellow-coloured and rod shaped bacterium, motile with polar flagellum. It was able to grow at 20-37 °C, at pH 4.5-9.0 and with 0-3 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DKC-1T formed a clade within the members of the genus Dyella and showed highest sequence similarities to Dyella japonica XD53T (98...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920842/marivibrio-halodurans-gen-nov-sp-nov-a-marine-bacterium-in-the-family-rhodospirillaceae-isolated-from-underground-rock-salt
#13
Shaoxing Chen, Yao Xu, Chen Zheng, Li-Xia Ke
Gram-negative, spiral or curved rod-shaped cells of a bacterial strain, designated ZC80T, were isolated from a rock salt sample collected at Yunnan salt mine, China. Analysis of the strain's 16S rRNA gene sequence revealed a clear affiliation of this novel strain within the family Rhodospirillaceae. Strain ZC80T formed a robust cluster with Pelagibius litoralis CL-UU02T at a 16S rRNA gene sequence similarity level of 88.1 %. Strain ZC80T shared no more than 91.0 % 16S rRNA gene sequence similarity with the type strains of other species in the family Rhodospirillaceae...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920841/desertibacillus-haloalkaliphilus-gen-nov-sp-nov-isolated-from-a-saline-desert
#14
Hitarth B Bhatt, M Azmatunnisa Begum, Sasikala Chintalapati, Venkata Ramana Chintalapati, Satya P Singh
Two Gram-stain-positive, rod-shaped and endospore-forming bacteria that represent a single species, designated strains KJ1-10-99T and KJ1-10-93, were isolated from a saline desert of Little Rann of Kutch, Gujarat, India. Analysis of 16S rRNA gene sequences revealed that the isolates belonged to the family Bacillaceae and were closely related to each other with 16S rRNA gene sequence similarity of 99.9 %. However, these two isolates formed a novel phylogenetic branch within this family. Both strains were aerobic, catalase and oxidase positive, and could grow optimally at 37 °C and pH 9...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920840/flavobacterium-dispersum-sp-nov-isolated-from-a-freshwater-spring
#15
Wen-Ming Chen, Ching-Ling Su, Shih-Yi Sheu
A novel bacterial strain MVW-23T was isolated from a freshwater spring in Taiwan. The strain was Gram-staining-negative, strictly aerobic, motile by gliding, rod-shaped and formed translucent yellow colonies. Optimal growth occurred at 20-30 °C, pH 7.0, and in the presence of 0.5-1 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-23T belonged to the genus Flavobacterium and showed the highest levels of sequence similarity with respect to Flavobacterium denitrificans ED5T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920839/natronotalea-proteinilytica-gen-nov-sp-nov-and-longimonas-haloalkaliphila-sp-nov-extremely-haloalkaliphilic-members-of-the-phylum-rhodothermaeota-from-hypersaline-alkaline-lakes
#16
Dimitry Y Sorokin, Tatiana V Khijniak, Erwin A Galinski, Ilya V Kublanov
Two proteolytic bacterial strains, BSker2T and BSker3T, were enriched from sediments of hypersaline alkaline lakes in Kulunda Steppe (Altai, Russia) with chicken feathers as substrate, followed by pure culture isolation on hypersaline alkaline media with casein. The cells were non-motile, filamentous, flexible rods. The isolates were obligately aerobic heterotrophs utilizing proteins and peptides as growth substrates. Both were obligate alkaliphiles, but differed in their pH optimum for growth: pH 9.5-9.8 for Bsker2T and pH 8...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920838/edaphobaculum-flavum-gen-nov-sp-nov-a-member-of-family-chitinophagaceae-isolated-from-grassland-soil
#17
Min Cao, Jing Huang, Jingxin Li, Zixu Qiao, Gejiao Wang
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterium, designated 1-116T, was isolated from Erdos grassland soil of Inner Mongolia, PR China. Phylogenetic analysis based on 16S rRNA genes showed that strain 1-116T was a member of family Chitinophagaceae and exhibited the highest similarities to Taibaiella koreensis THG-DT86T (90.1 %) and Flavihumibacter solisivae 3-3T (90.0 %), while the similarities to the other Chitinophagaceae type strains were lower than 90.0 %...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920837/bacillus-solisilvae-sp-nov-isolated-from-forest-soil
#18
Tong Pan, Hairong He, Xiaochong Wang, Yibo Shen, Junwei Zhao, Kai Yan, Xiangjing Wang, Chongxi Liu, Ji Zhang, Wensheng Xiang
A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterial strain, NEAU-cbsb5T, was isolated from forest soil from Changbai Mountain, Heilongjiang Province, China. The isolate grew at 15-40 °C (optimum 30 °C), at pH 6.0-8.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl, although NaCl was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-cbsb5T formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus acidiceler DSM 18954T (99...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920835/erythrobacter-arachoides-sp-nov-isolated-from-ice-core
#19
Tingting Xing, Yongqin Liu, Ninglian Wang, Baiqing Xu, Keshao Liu, Liang Shen, Zhengquan Gu, Bixi Guo, Yuguang Zhou, Hongcan Liu
A Gram-stain-negative, rod-shaped bacterial strain, designed RC4-10-4T, belonging to the genus Erythrobacter, was isolated from the East Rongbuk Glacier on the Tibetan Plateau. Strain RC4-10-4T grew optimally at pH 7.0, at 25 °C and in the presence of 2 % (w/v) NaCl. Summed feature 3 (C16 : 1 ω6c and/or iso-C15 : 0 2-OH), summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C16 : 0 were the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid and phosphatidylcholine...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920832/salinifilum-gen-nov-with-description-of-salinifilum-proteinilyticum-sp-nov-an-extremely-halophilic-actinomycete-isolated-from-meighan-wetland-iran-and-reclassification-of-saccharopolyspora-aidingensis-as-salinifilum-aidingensis-comb-nov-and-saccharopolyspora
#20
Mahdi Moshtaghi Nikou, Mohaddaseh Ramezani, Sharareh Harirchi, Somayyeh Makzoom, Mohammad Ali Amoozegar, Seyed Abolhassan Shahzadeh Fazeli, Peter Schumann, Antonio Ventosa
A Gram-positive, halophilic actinobacterial strain Miq-12T was isolated from Meighan wetland in Iran. Strain Miq-12T was strictly aerobic, catalase positive and oxidase negative. The isolate grew at 12-25 % NaCl, at 30-50 °C and pH 5.5-10.5. The optimum NaCl, temperature and pH for growth were 15-20 %, 40 °C and 7.0-8.0, respectively. The cell wall of strain Miq-12T contained meso-diaminopimelic acid as diagnostic diamino acid and arabinose as whole-cell sugar. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
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