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https://www.readbyqxmd.com/read/28820127/mongoliimonas-terrestris-gen-nov-sp-nov-isolated-from-desert-soil
#1
Jiao Xi, Yu Wang, Xiaorong Yang, Yu Tao, Yufang Shao, Fuying Feng
A Gram-stain-negative, non-motile, aerobic, non-spore-forming, spherical bacterium (strain MIMtkB18T) was isolated from desert soil collected from part of a Mongolian Plateau, territory of Inner Mongolia, PR China. Cell growth could be observed at 20-45 °C (optimum at 40 °C), at a pH of 6-9 (optimum at pH 8.6) and in the presence of 0-1 % (w/v) NaCl (optimum 0 %). The genomic DNA G+C content was 69.6 mol%. 16S rRNA gene sequence analysis showed that strain MIMtkB18T was most closely related to Methylobrevis pamukkalensis PK2T (94...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820126/adhaeribacter-terrae-sp-nov-a-novel-bacterium-isolated-from-soil
#2
Nabil Elderiny, Jae-Jin Lee, Yeon-Hee Lee, Su-Jin Park, Seung-Yeol Lee, Sangkyu Park, Young-Je Cho, Leonid N Ten, Hee-Young Jung
Strain HY02T was isolated from a soil sample collected at Namyangju-si, Gyeonggi-do, Republic of Korea. Cells of this strain were observed to be Gram-stain-negative, short and rod-shaped. Colonies were red in colour. A 16S rRNA gene sequence analysis identified this strain as a member of the genus Adhaeribacter in the family Cytophagaceae, with the highest level of 16S rRNA gene sequence similarity to Adhaeribacter terreus DNG6T (98.08 %). This strain was positive for oxidase but negative for catalase activity and acid production from glucose...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820124/bacillus-wudalianchiensis-sp-nov-isolated-from-grass-soils-of-the-wudalianchi-scenic-area
#3
Bo Liu, Guo-Hong Liu, Cetin Sengonca, Peter Schumann, Jie-Ping Wang, Yu-Jing Zhu, Hai-Feng Zhang
A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-27215T, was isolated from grass soil collected from Wudalianchi in the Heilongjiang Province of China. Growth was observed at 10-60 °C (optimum 30 °C), in 0 and 3.0 % NaCl (optimum 0 %) and at pH 5.0-10.0 (optimum 7.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK7. The main fatty acids were iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 0, and iso-C16 : 0...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820121/aureimonas-endophytica-sp-nov-a-novel-endophytic-bacterium-isolated-from-aegiceras-corniculatum
#4
Fei-Na Li, Li Tuo, Zhen Pan, Min Guo, Simon Ming-Yuen Lee, Li Chen, Lin Hu, Cheng-Hang Sun
A Gram-negative, motile, aerobic and coccoid rod-shaped bacterium, designated strain 2T4P-2-4T, was isolated from a piece of surface-sterilized bark of Aegiceras corniculatum collected from Cotai Ecological Zones in Macao, China, and tested by a polyphasic approach to clarify its taxonomic position. Strain 2T4P-2-4T grew optimally without NaCl at 28-30 °C, pH 7.0-8.0. The 16S rRNA gene sequence of strain 2T4P-2-4T had the highest similarity (96.2 %) to Aureimonas rubiginis CC-CFT034T. Phylogenetic analysis showed that the strain grouped with species of the genus Aureimonas...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820118/arcobacter-haliotis-sp-nov-isolated-from-abalone-species-haliotis-gigantea
#5
Reiji Tanaka, Ilse Cleenwerck, Yukino Mizutani, Shunpei Iehata, Peter Bossier, Peter Vandamme
A Gram-negative, aerobic, polar-flagellated and rod-shaped, sometimes slightly curved bacterium, designated MA5T, was isolated from the gut of an abalone of the species Haliotis gigantea collected in Japan. Phylogenetic analyses based on 16S rRNA, gyrB, hsp60 and rpoB gene sequences placed strain MA5T in the genus Arcobacter in an independent phylogenetic line. Comparison of the 16S rRNA gene sequence of this strain with those of the type strains of the established Arcobacter species revealed A. nitrofigilis (95...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820117/mucilaginibacter-rubeus-sp-nov-isolated-from-rhizosphere-soil
#6
Jie-Chao Wei, Le-Ni Sun, Zhuo-Xin Yuan, Xue-Ting Hou, En-Dong Yang, Yuan-Yuan Cao
A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated EF23T, was isolated from rhizosphere soil of watermelon. Growth of strain EF23T was observed at 10-37 °C, at pH 5.0-9.0 and in the presence of 0-0.5 % (w/v) NaCl. Strain EF23T contained menaquinone 7 (MK-7) as the major isoprenoid quinone, and summed feature 3 (C16:1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH as the major fatty acids. Phosphatidylethanolamine was identified as the major polar lipid...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820116/sphingobium-naphthae-sp-nov-with-the-ability-to-degrade-aliphatic-hydrocarbons-isolated-from-oil-contaminated-soil
#7
Dhiraj Kumar Chaudhary, Seung-Woo Jeong, Jaisoo Kim
A light yellow-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain K-3-6T, capable of degrading aliphatic hydrocarbons was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal. It was able to grow at 15-45 °C, at pH 5.0-9.5 and with 0-6 % (w/v) NaCl. Based on 16S rRNA gene sequence analysis, strain K-3-6T belongs to the genus Sphingobium and is closely related to Sphingobium olei IMMIB HF-1T (98.4 % similarity), Sphingobium abikonense NBRC 16140T (98.3 %), Sphingobium rhizovicinum CC-FH12-1T (97...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820108/nakamurella-intestinalis-sp-nov-isolated-from-the-faeces-of-pseudorhynchus-japonicus
#8
Soo-Jin Kim, Hayoung Cho, Jae-Ho Joa, Moriyuki Hamada, Jae-Hyung Ahn, Hang-Yeon Weon, Soon-Wo Kwon
One strain, designated 63MJ-1T, was isolated from fresh faeces of broad-winged katydids collected in Jinan-gun, Jeollabuk-do, the Republic of Korea. The organism stained Gram-positive and was an aerobic, non-flagellated and short-rod-shaped bacterium. The organism grew in the range of 4-35 °C (optimum, 28-30 °C) and pH 6.0-9.0 (optimum, pH 7.0), and in the presence of 5 % NaCl (w/v), but not in media containing 7 % NaCl. According to the 16S rRNA gene sequence analysis, strain 63MJ-1T showed the highest sequence similarities with Nakamurella panacisegetis P4-7T (95...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820105/roseomonas-hibiscisoli-sp-nov-isolated-from-the-rhizosphere-of-mugunghwa-hibiscus-syriacus
#9
Zheng-Fei Yan, Pei Lin, Chang-Tian Li, MooChang Kook, Qi-Jun Wang, Tae-Hoo Yi
A Gram-stain-negative, aerobic, non-motile and coccoid to short-rod-shaped bacterial strain (THG-N2.22T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 20-40 °C (optimum 28 °C), at pH 5-9 (optimum 7) and with 0-4 % (w/v) NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-N2.22T were identified as Roseomonas rhizosphaerae YW11T (98.5 % similarity), Roseomonas rubra S5T (98.5 %), Roseomonas cervicalis ATCC 49957T (98...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820102/umboniibacter-caenipelagi-sp-nov-isolated-from-a-tidal-flat
#10
Sooyeon Park, Su Jung Choi, Ji-Min Park, Jung-Hoon Yoon
A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated SCTF-16T, was isolated from a tidal flat on the Yellow Sea, South Korea. Strain SCTF-16T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2.0 % (w/v) NaCl. In the neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences, strain SCTF-16T clustered with the type strains of two species of the genus Umboniibacter. Strain SCTF-16T exhibited 16S rRNA gene sequence similarities of 99...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820100/erythrobacter-aquimixticola-sp-nov-isolated-from-the-junction-between-the-ocean-and-a-freshwater-spring
#11
Sooyeon Park, Yong-Taek Jung, Su Jung Choi, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated JSSK-14T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-14T grew optimally at pH 7.0-8.0, at 30 °C and in the presence of 1.0-2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain JSSK-14T fell within the clade comprising the type strains of species of the genus Erythrobacter. Strain JSSK-14T exhibited 16S rRNA gene sequence similarity values of 97...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820099/hymenobacter-pallidus-sp-nov-isolated-from-a-freshwater-fish-culture-pond
#12
Shih-Yi Sheu, Yi-Shu Li, Chiu-Chung Young, Wen-Ming Chen
A bacterial strain designated LYH-12T was isolated from a freshwater fish culture pond in Taiwan, ROC and characterized by taking a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain LYH-12T belonged to the genus Hymenobacter and was most closely related to Hymenobacter xinjiangensis X2-1gT and Hymenobacter rigui WPCB131T with a sequence similarity of 96.6 % and less than 96.5 % with other members of the genus. Cells of strain LYH-12T were Gram-stain-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink-coloured colonies...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820089/alteromonas-aestuariivivens-sp-nov-isolated-from-a-tidal-flat
#13
Sooyeon Park, Su Jung Choi, Ji-Min Park, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-spore-forming, motile and ovoid or rod-shaped bacterial strain, JDTF-113T, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-113T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain JDTF-113T fell within the clade enclosing the type strains of species of the genus Alteromonas. Strain JDTF-113T exhibited 16S rRNA gene sequence similarity values of 97...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820088/bacillus-praedii-sp-nov-isolated-from-purplish-paddy-soil
#14
Bo Liu, Guo-Hong Liu, Cetin Sengonca, Peter Schumann, Jie-Ping Wang, Yu-Jing Zhu, Qin-Ying Liu, Ming-Kuang Wang
A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated strain FJAT-25547T, was isolated from the purplish paddy soil collected from Linshan Township, Yanting Prefecture of Sichuan Province in PR China (31° 16' N 105° 27' E). Growth was achieved aerobically at temperatures between 15 and 40 °C (optimum 30 °C), with between 0 and 10.0 % NaCl (w/v) (optimum 4 %) and in the range of pH 5.0-12.0 (optimum pH 9.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid, and the main isoprenoid quinone was MK-7...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820072/structural-design-and-physicochemical-foundations-of-hydrogels-for-biomedical-and-pharmaceutical-applications
#15
Qingyong Li, Wen Li, Jie Xu, Zhengxiang Ning, Jiaoyan Ren, Wenzhen Liao
Biomedical research, known as medical research, is conducive to support and promote the development of knowledge in the field of medicine. Hydrogels have been extensively used in many biomedical fields due to theirs highly absorbent and flexible properties. The smart hydrogels, especially, can respond to a broad range of external stimuli such as temperature, pH value, light, electric and magnetic fields. With excellent biocompatibility, tunable rheology, mechanical properties, porosity, and hydrated molecular structure, hydrogels are considered as promising candidate for simulating local tissue microenvironment...
August 17, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28819794/in-vitro-selection-and-identification-of-potential-probiotics-isolated-from-the-gastrointestinal-tract-of-nile-tilapia-oreochromis-niloticus
#16
Rasha M Reda, Khaled M Selim, Hassanin M El-Sayed, M A El-Hady
Fish gut bacteria can be used as probiotics for aquaculture. The aim of this study is to screen and identify beneficial probiotic bacteria from the gut of Nile tilapia, Oreochromis niloticus. Nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, Aeromonas sobria, Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas putida, and Staphylococcus aureus. Further tests showed that such isolates can survive in the presence of high bile concentration (10%) and at different acidic pH values...
August 17, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28819743/halorubrum-salsamenti-sp-nov-a-novel-halophilic-archaeon-isolated-from-a-brine-of-salt-mine
#17
Shaoxing Chen, Jiao He, Jiao Zhang, Yao Xu, Jiao Huang, Li-Xia Ke
A non-motile, spherical or oval extremely halophilic archaeon, strain Y69(T), was isolated from a brine of the Yunnan salt mine, China. Colonies on JCM 168 agar plate were round (1-2 mm in diameter), moist, and orange-pigmented. Phylogenetic analysis of the almost-complete 16S rRNA gene sequence showed that the isolate belonged to the species of the genus Halorubrum, with a close relationship to Halorubrum aidingense 31-hong(T) (98.5%), Halorubrum lacusprofundi ATCC 49239(T) (98.2%), and Halorubrum kocurii BG-1(T) (98...
August 17, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28819159/identification-of-boric-acid-as-a-novel-chemoattractant-and-elucidation-of-its-chemoreceptor-in-ralstonia-pseudosolanacearum-ps29
#18
Akiko Hida, Shota Oku, Yutaka Nakashimada, Takahisa Tajima, Junichi Kato
Chemotaxis enables bacteria to move toward more favorable environmental conditions. We observed chemotaxis toward boric acid by Ralstonia pseudosolanacearum Ps29. At higher concentrations, the chemotactic response of R. pseudosolanacearum toward boric acid was comparable to or higher than that toward L-malate, indicating that boric acid is a strong attractant for R. pseudosolanacearum. Chemotaxis assays under different pH conditions suggested that R. pseudosolanacearum recognizes B(OH)3 (or B(OH3) + B(OH)4(-)) but not B(OH)4(-) alone...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28816641/preparation-of-recombinant-human-thymic-stromal-lymphopoietin-protein
#19
Yanlu Zhang, Liehua Wu, Jingai Yu, JianFeng Mei, Yu Yi, JianShu Chen, Guoqing Ying
Human thymic stromal lymphopoietin (hTSLP) protein plays a central role in inflammation. Characterizing properties of hTSLP requires a recombinant overexpression system that produces correctly folded, active hTSLP. In this report, an efficient overexpression system for production of hTSLP was developed. We constructed expression plasmids of the full-length hTslp gene with or without the signal peptide and transformed the plasmids into E. coli. The design of the recombinant proteins included an N-terminal His-tag, which facilitated purification...
August 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28815328/microbial-diversity-in-acidic-thermal-pools-in-the-uzon-caldera-kamchatka
#20
Andrey V Mardanov, Vadim M Gumerov, Alexey V Beletsky, Nikolai V Ravin
Microbial communities of four acidic thermal pools in the Uzon Caldera, Kamchatka, Russia, were studied using amplification and pyrosequencing of 16S rRNA gene fragments. The sites differed in temperature and pH: 1805 (60 °C, pH 3.7), 1810 (90 °C, pH 4.1), 1818 (80 °C, pH 3.5), and 1807 (86 °C, pH 5.6). Archaea of the order Sulfolobales were present among the dominant groups in all four pools. Acidilobales dominated in pool 1818 but were a minor fraction at the higher temperature in pool 1810. Uncultivated Archaea of the Hot Thaumarchaeota-related clade were present in significant quantities in pools 1805 and 1807, but they were not abundant in pools 1810 and 1818, where high temperatures were combined with low pH...
August 16, 2017: Antonie Van Leeuwenhoek
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