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https://www.readbyqxmd.com/read/28214441/enhanced-treatment-of-fischer-tropsch-f-t-wastewater-using-the-up-flow-anaerobic-sludge-blanket-coupled-with-bioelectrochemical-system-effect-of-electric-field
#1
Dexin Wang, Hongjun Han, Yuxing Han, Kun Li, Hao Zhu
The coupling of bioelectrochemical system (BES) with an up-flow anaerobic sludge blanket (UASB) was established for enhanced Fischer-Tropsch (F-T) wastewater treatment while the UASB (control group) was operated in parallel. The presence of electric field could offer system a more reductive micro-environment that lower the ORP values and maintain the appropriate pH range, resulting in the higher chemical oxygen demand (COD) removal efficiency and methane production for BES-UASB (86.8% and 2.31±0.1L/(L·d)) while those values in control group were 72...
February 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214187/microbiological-analysis-antimicrobial-activity-and-heavy-metals-content-of-jordanian-ma-in-hot-springs-water
#2
Muhamad Ali K Shakhatreh, Jacob H Jacob, Emad I Hussein, Majed M Masadeh, Safwan M Obeidat, Abdul-Salam F Juhmani, Mutaz A Abd Al-Razaq
Ma'in hot springs are known as sites of balneotherapy. However, little is known about their microbiology and chemistry. In this study, we aim at evaluating the antimicrobial activity of Ma'in hot-springs water (MHSW), studying its microbiology, and determining its physicochemical properties including the heavy metals content. Therefore, water samples were collected from Ma'in hot springs and tested for antimicrobial activity using agar diffusion method. Water was then cultivated on nutrient agar to isolate and identify the dominant bacteria by chemical and molecular methods...
February 14, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28214040/reinforcement-of-intestinal-epithelial-barrier-by-arabinoxylans-in-overweight-and-obese-subjects-a-randomized-controlled-trial-arabinoxylans-in-gut-barrier
#3
Bouke N Salden, Freddy J Troost, Ellen Wilms, Pilar Truchado, Ramiro Vilchez-Vargas, Dietmar H Pieper, Ruy Jáuregui, Massimo Marzorati, Tom van de Wiele, Sam Possemiers, Ad A Masclee
BACKGROUND & AIMS: Obesity and metabolic diseases are associated with alterations in microbial composition and impaired gut barrier. Previous in vitro and animal studies have shown that arabinoxylans (AX) have the potential to modulate gut microbiota and gut barrier and therefore could have a protective role. Primary aim of the study was to investigate the effect of AX on intestinal permeability. Secondary aims included the effect of AX on gene transcription and protein expression of tight junctions (TJ), intestinal microbiota composition and activity, immune response and metabolic markers in overweight and obese individuals...
February 3, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28213880/detection-of-digestive-malignancies-and-post-gastrectomy-complications-via-gastrointestinal-fluid-examination
#4
REVIEW
Lei Huang, Aman Xu
To date, gastric carcinoma (GC) is one of the common and fatal digestive malignancies worldwide. The prognosis of GC is not always satisfactory because of late diagnosis. Scholars are keen on discovering novel accurate and economical biomarkers in body liquids for GC screening to detect and evaluate the lesion before the results of imaging techniques are obtained. While traditional serum assays have limited sensitivity and specificity, gastrointestinal juice may provide relevant specific biomarkers because of its close contact with the tumor...
February 17, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28213710/biohydrogen-production-from-sugarcane-bagasse-hydrolysate-effects-of-ph-s-x-fe-2-and-magnetite-nanoparticles
#5
Karen Reddy, Mahmoud Nasr, Sheena Kumari, Santhosh Kumar, Sanjay Kumar Gupta, Abimbola Motunrayo Enitan, Faizal Bux
Batch dark fermentation experiments were conducted to investigate the effects of initial pH, substrate-to-biomass (S/X) ratio, and concentrations of Fe(2+) and magnetite nanoparticles on biohydrogen production from sugarcane bagasse (SCB) hydrolysate. By applying the response surface methodology, the optimum condition of steam-acid hydrolysis was 0.64% (v/v) H2SO4 for 55.7 min, which obtained a sugar yield of 274 mg g(-1). The maximum hydrogen yield (HY) of 0.874 mol (mol glucose(-1)) was detected at the optimum pH of 5...
February 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28213662/desulfobulbus-aggregans-sp-nov-a-novel-sulfate-reducing-bacterium-isolated-from-marine-sediment-from-the-gulf-of-gabes
#6
Hanen Kharrat, Fatma Karray, Manon Bartoli, Wajdi Ben Hnia, Najla Mhiri, Marie-Laure Fardeau, Feten Bennour, Lotfi Kamoun, Didier Alazard, Sami Sayadi
Three sulfate-reducing bacterial strains designated SM40(T), SM41, and SM43 were isolated from marine sediment in the region of Skhira located in the Gulf of Gabes (Tunisia). These strains grew in anaerobic media with phosphogypsum as a sulfate source and sodium lactate as an electron and carbon source. One of them, strain SM40(T), was characterized by phenotypic and phylogenetic methods. Cells were ovoid, Gram-stain-negative and non-motile. The temperature limits for growth were 10 and 55 °C with an optimum at 35 °C and the pH range was 6...
February 17, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28213542/plant-community-and-nitrogen-deposition-as-drivers-of-%C3%AE-and-%C3%AE-prokaryotic-diversity-in-reconstructed-oil-sands-soils-and-natural-boreal-forest-soils
#7
Jacynthe Masse, Cindy E Prescott, Sébastien Renaut, Yves Terrat, Sue J Grayston
The Athabasca Oil Sands deposit is one of the largest single oil deposits in the world. Following surface mining, companies are required to restore soil-like profiles that can support the previous land capabilities. The objective of this study was to assess if soil prokaryotic α- and β-diversity in oil-sands soils reconstructed 20-30 years previously, and planted to one of three vegetation types (coniferous or deciduous trees and grassland) were similar to those found in natural boreal-forest soils subject to wildfire disturbance...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28212663/isolation-of-primary-microglia-from-the-human-post-mortem-brain-effects-of-ante-and-post-mortem-variables
#8
Mark R Mizee, Suzanne S M Miedema, Marlijn van der Poel, Adelia, Karianne G Schuurman, Miriam E van Strien, Jeroen Melief, Joost Smolders, Debbie A Hendrickx, Kirstin M Heutinck, Jörg Hamann, Inge Huitinga
Microglia are key players in the central nervous system in health and disease. Much pioneering research on microglia function has been carried out in vivo with the use of genetic animal models. However, to fully understand the role of microglia in neurological and psychiatric disorders, it is crucial to study primary human microglia from brain donors. We have developed a rapid procedure for the isolation of pure human microglia from autopsy tissue using density gradient centrifugation followed by CD11b-specific cell selection...
February 17, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28211312/olivibacter-composti-sp-nov-isolated-from-compost-collected-at-a-greenhouse
#9
Shih-Yao Lin, Asif Hameed, Kai-Yeh Chiu, You-Cheng Liu, Yi-Han Hsu, Wei-An Lai, Chiu-Chung Young
A polyphasic approach was used to characterize a presumably novel bacterium, designated strain CC-KYC063T, isolated from a compost sample collected at a greenhouse facility within Taiwan. Strain CC-KYC063T was Gram-stain-negative and aerobic, able to grow at 15-37 °C (optimal 30 °C), at pH 7.0-9.0 (optimal pH 7.0) and with 0-2 % (w/v) NaCl. The 16S rRNA gene sequence of strain CC-KYC063T showed highest similarity to Olivibacter ginsengisoli Gsoil 060T (96.2 %) and Olivibacter terrae Jip13T (95.5 %)...
January 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28211311/oleiagrimonas-citrea-sp-nov-a-marine-bacterium-isolated-from-tidal-flat-sediment-and-emended-description-of-the-genus-oleiagrimonas-fang-et-al-2015-and-oleiagrimonas-soli
#10
Sung-Hyun Yang, Hyun-Seok Seo, Chi Nam Seong, Kae Kyoung Kwon
A Gram-negative, aerobic, rod-shaped (1.8-2.3 μm  0.4-0.6 μm), and motile marine bacterium, designated as MEBiC09124T was isolated from tidal flat sediment of the Suncheon Bay, South Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC09124T showed high similarity with the Oleiagrimonas soli 3.5XT (96.7%). Growth was observed at 18-38 OC (optimum 30 OC), at pH 4.0-8.5 (optimum pH 7.5) and with 0-6 % (optimum 2.5%) NaCl. The predominant cellular fatty acids were iso-C15 : 0 (22.6%), iso-C16 : 0 (16...
February 3, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28211309/chryseobacterium-reticulitermitis-sp-nov-isolated-from-the-gut-of-reticulitermes-aculabialis
#11
Yun Zhao, Yu Wang, Dan Hong Li, Yu Deng, Hong Yang
A Gram-staining-negative, non-motile, aerobic and rod-shaped bacterium, strain Ra1T, was isolated from the gut of a wood-feeding lower termite, Reticulitermes aculabialis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was closely related to Chryseobacterium rigui JCM 18078T (96.7%). Growth was observed at 15-45℃ (optimum 30℃), at pH 6.0-9.0 (optimum pH 8.0) and in the presence of 0-2% NaCl (optimum 0%). The DNA G+C content of strain Ra1T was 39.9 mol%. The cells contained MK-6 as the sole respiratory quinone and the major fatty acids were iso-C15:0, iso-C17:0, summed feature 3 (comprising C16:1ω6c and/or C16:1ω7c) and summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1ω9c)...
February 2, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28210252/compositions-and-abundances-of-sulfate-reducing-and-sulfur-oxidizing-microorganisms-in-water-flooded-petroleum-reservoirs-with-different-temperatures-in-china
#12
Huimei Tian, Peike Gao, Zhaohui Chen, Yanshu Li, Yan Li, Yansen Wang, Jiefang Zhou, Guoqiang Li, Ting Ma
Sulfate-reducing bacteria (SRB) have been studied extensively in the petroleum industry due to their role in corrosion, but very little is known about sulfur-oxidizing bacteria (SOB), which drive the oxidization of sulfur-compounds produced by the activity of SRB in petroleum reservoirs. Here, we surveyed the community structure, diversity and abundance of SRB and SOB simultaneously based on 16S rRNA, dsrB and soxB gene sequencing, and quantitative PCR analyses, respectively in petroleum reservoirs with different physicochemical properties...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28209995/rna-seq-based-transcriptomic-and-metabolomic-analysis-reveal-stress-responses-and-programmed-cell-death-induced-by-acetic-acid-in-saccharomyces-cerevisiae
#13
Yachen Dong, Jingjin Hu, Linlin Fan, Qihe Chen
As a typical harmful inhibitor in cellulosic hydrolyzates, acetic acid not only hinders bioethanol production, but also induces cell death in Saccharomyces cerevisiae. Herein, we conducted both transcriptomic and metabolomic analyses to investigate the global responses under acetic acid stress at different stages. There were 295 up-regulated and 427 down-regulated genes identified at more than two time points during acetic acid treatment (150 mM, pH 3.0). These differentially expressed genes (DEGs) were mainly involved in intracellular homeostasis, central metabolic pathway, transcription regulation, protein folding and stabilization, ubiquitin-dependent protein catabolic process, vesicle-mediated transport, protein synthesis, MAPK signaling pathways, cell cycle, programmed cell death, etc...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28209394/ensifer-shofinae-sp-nov-a-novel-rhizobial-species-isolated-from-root-nodules-of-soybean-glycine-max
#14
Wen Hao Chen, Sheng Hui Yang, Zhao Hu Li, Xiao Xia Zhang, Xin Hua Sui, En Tao Wang, Wen Xin Chen, Wen Feng Chen
Two bacterial strains isolated from root nodules of soybean were characterized phylogenetically as members of a distinct group in the genus Ensifer based on 16S rRNA gene comparisons. They were also verified as a separated group by the concatenated sequence analyses of recA, atpD and glnII (with similarities ≤93.9% to the type strains for defined species), and by the average nucleotide identities (ANI) between the whole genome sequence of the representative strain CCBAU 251167(T) and those of the closely related strains in Ensifer glycinis and Ensifer fredii (90...
February 2, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28203227/some-technological-properties-of-lactic-acid-bacteria-isolated-from-dahi-and-datshi-naturally-fermented-milk-products-of-bhutan
#15
H N J Shangpliang, Sharmila Sharma, Ranjita Rai, Jyoti P Tamang
Dahi and datshi are common naturally fermented milk (NFM) products of Bhutan. Population of lactic acid bacteria (LAB) in dahi (pH 3.7) and datshi (pH 5.2) was 1.4 × 10(7) and 3.9 × 10(8) cfu/ml, respectively. Based on 16S rRNA gene sequencing isolates of LAB from dahi and datshi were identified as Enterococcus faecalis, E. faecium, Lactococcus lactis subsp. lactis. LAB strains were tested for some technological properties. All LAB strains except E. faecalis CH2:17 caused coagulation of milk at both 30°C for 48 h...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28199317/colorimetric-tests-for-diagnosis-of-filarial-infection-and-vector-surveillance-using-non-instrumented-nucleic-acid-loop-mediated-isothermal-amplification-nina-lamp
#16
Catherine B Poole, Zhiru Li, Andy Alhassan, Dylan Guelig, Steven Diesburg, Nathan A Tanner, Yinhua Zhang, Thomas C Evans, Paul LaBarre, Samuel Wanji, Robert A Burton, Clotilde K S Carlow
Accurate detection of filarial parasites in humans is essential for the implementation and evaluation of mass drug administration programs to control onchocerciasis and lymphatic filariasis. Determining the infection levels in vector populations is also important for assessing transmission, deciding when drug treatments may be terminated and for monitoring recrudescence. Immunological methods to detect infection in humans are available, however, cross-reactivity issues have been reported. Nucleic acid-based molecular assays offer high levels of specificity and sensitivity, and can be used to detect infection in both humans and vectors...
2017: PloS One
https://www.readbyqxmd.com/read/28199124/tempo-spatial-activation-of-sequential-quadruple-stimuli-for-high-gene-expression-of-polymeric-gene-nanocomplexes
#17
Hana Cho, Young-Woo Cho, Sun-Woong Kang, Mi-Kyoung Kwak, Kang Moo Huh, You Han Bae, Han Chang Kang
The clinical application of intracellular gene delivery via nanosized carriers is hindered by intracellular multistep barriers that limit high levels of gene expression. To solve these issues, four different intracellular or external stimuli that can efficiently activate a gene carrier, a gene, or a photosensitizer (pheophorbide A [PhA]) were assessed in this study. The designed nanosized polymeric gene complexes were composed of PhA-loaded thiol-degradable polycation (PhA@RPC) and cytomegalovirus (CMV) promoter-equipped pDNA...
February 15, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28198807/paenibacillus-polymyxa-nsy50-suppresses-fusarium-wilt-in-cucumbers-by-regulating-the-rhizospheric-microbial-community
#18
Lu Shi, Nanshan Du, Sheng Shu, Jin Sun, Shuzhan Li, Shirong Guo
Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2)...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28198453/doxorubicin-loaded-platelets-as-a-smart-drug-delivery-system-an-improved-therapy-for-lymphoma
#19
Peipei Xu, Huaqin Zuo, Bing Chen, Ruju Wang, Arsalan Ahmed, Yong Hu, Jian Ouyang
Chemotherapy is majorly used for the treatment of many cancers, including lymphoma. However, cytotoxic drugs, utilized in chemotherapy, can induce various side effects on normal tissues because of their non-specific distribution in the body. Natural platelets are used as drug carriers because of their biocompatibility and specific targeting to vascular disorders, such as cancer, inflammation, and thrombosis. In this work, doxorubicin (DOX) was loaded in natural platelets for treatment of lymphoma. Results showed that DOX was loaded into platelets with high drug loading and encapsulation efficiency...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28198096/treated-dentin-matrix-paste-as-a-novel-pulp-capping-agent-for-dentin-regeneration
#20
Jinlong Chen, Caiyun Cui, Xiangchen Qiao, Bo Yang, Mei Yu, Weihua Guo, Weidong Tian
Regenerating dentin and preserving pulp vitality are the two key targets for the treatment of dental pulp exposure. Calcium hydroxide (CH), the widely used capping agent, may induce potential tunnel defect in reparative dentin and cause inflammation or even necrosis in pulp tissues. This study aimed to produce a novel pulp capping agent with better bioactivities. Treated dentin matrix (TDM) paste (TDMP) was fabricated consisting of TDM powder and aqueous TDM extract. The chemical and biological characteristics of TDMP were investigated, and its effect on the odontogenic differentiation of dental pulp stem cells explored at gene and protein level; the therapeutic effect for pulp exposure in miniature swine was further verified...
February 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
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