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Li Xie, Liqin Chen, Pan Gu, Lanlan Wei, Xuejun Kang
The extraction and analysis of catecholamine neurotransmitters in biological fluids is of great importance in assessing nervous system function and related diseases, but their precise measurement is still a challenge. Many protocols have been described for neurotransmitter measurement by a variety of instruments, including high-pressure liquid chromatography (HPLC). However, there are shortcomings, such as complicated operation or hard-to-detect multiple targets, which cannot be avoided, and presently, the dominant analysis technique is still HPLC coupled with sensitive electrochemical or fluorimetric detection, due to its high sensitivity and good selectivity...
March 1, 2018: Journal of Visualized Experiments: JoVE
Chang-Gu Lee, Hassan Javed, Danning Zhang, Jae-Hong Kim, Paul Westerhoff, Qilin Li, Pedro J J Alvarez
Using a bi-polymer system consisting of polyvinylpyrrolidone (PVP) and poly(vinylidene fluoride) (PVDF), P25-TiO2 was immobilized into thin film mats of porous electrospun fibers. Pores were introduced by dissolving sacrificial PVP to increase surface area and enhance access to TiO2. The highest photocatalytic activity was achieved using a PVDF:PVP weight ratio of 2:1. Methylene blue (MB) was used to visualize contaminant removal, assess the sorption capacity (5.93 ± 0.23 mg/g) and demonstrate stable removal kinetics (kMB > 0...
March 19, 2018: Environmental Science & Technology
Hande Nalvuran, Ayşe Eser Elçin, Yaşar Murat Elçin
Graphene and silk fibroin (SF) have been extensively investigated in the literature. Hybrid scaffolds of SF and graphene combine the properties of both of the materials and provide promising applications for tissue engineering purposes. In this study, reduced graphene oxide (RGO) (0.5%, 1.0% and 2.0% (w/v)) was incorporated into SF and fabricated into composite nanofibers through electrospinning. The fibers were characterized and analyzed by SEM, XRD, FTIR, TGA, circular dichroism analysis, contact angle measurements and tensile tests...
March 15, 2018: International Journal of Biological Macromolecules
Shijie Li, Shiwei Hu, Wei Jiang, Yanping Liu, Yingtang Zhou, Yu Liu, Liuye Mo
A huge challenge in the field of pollutant removal is the scarcity of visible-light-driven (VLD) photocatalysts that are efficient, stable, easily recyclable and capable of mineralizing organic pollutants. In this regard, a novel hierarchical architecture of Bi2 MoO6 nanosheets onto NiTiO3 nanofibers for tetracycline hydrochloride (TC) removal was rationally designed and fabricated via a facile approach. In this heterojunction system, highly homogeneous-distributed Bi2 MoO6 nanosheets were anchored on electrospun NiTiO3 nanofibers, endowing the heterojunction with compact interfacial contact...
March 13, 2018: Journal of Colloid and Interface Science
Chen Zou, Xiaojuan Zhao, Yan Xu
We report a novel, one-dimensional zirconium-doped layered trititanate with a porous core and a textured surface of ultrafine nanofibers (∼5 nm) by the hydrothermal alkaline treatment of electrospun fibers. It demonstrates superior efficiency for rapid, capacitive and simultaneous removal of multiple heavy metal ions such as Pb2+, Cd2+, Cu2+ and Zn2+. The adsorption is exceptionally rapid, showing 100% removal of Cu2+ in 10 min, and 100% removal of Pb2+ and Cd2+ in 20 min in water with a wide range of concentrations from 0...
March 16, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Y J Son, H S Kim, W Mao, J B Park, D Lee, H Lee, H S Yoo
A two-step strategy for coaxial electrospinning and postelectrospinning is an effective method for fabricating superfine nanofibers composed of highly swellable hydrogels. Alginate and poly(ε-caprolactone) [PCL] were coelectrospun via fibrous meshes with a coaxial nozzle; alginate at the core was subsequently cross-linked in calcium chloride solution. The PCL sheath was removed from the meshes by repeated organic-phase washing. The peeling process was monitored by scanning electron microscopy, transmission electron microscopy, and differential scanning calorimetry, and the complete removal of the PCL outer layers was confirmed by the thinning of the fiber volume...
March 16, 2018: Nanoscale
Ali Deniz Dalgic, Ammar Zeidan Alshemary, Ayşen Tezcaner, Dilek Keskin, Zafer Evis
In this study, novel graphene oxide-incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide...
January 1, 2018: Journal of Biomaterials Applications
Deniz Turan, Monika Gibis, Gurbuz Gunes, Stefan K Baier, Jochen Weiss
The conjugation reaction of electrospun fibers of a mixture of whey protein isolate (WPI) and dextran using different molecular weights (40, 70, and 100 kDa) and mixing ratios was studied. This study includes the electrospinnability of a mixture of WPI and dextran, and the conjugation reaction between them via the initial stage of the Maillard reaction. The WPI-dextran fibers were characterized using optical and transmission electron microscopy. The covalent attachment of dextran to WPI was confirmed using sodium-dodecyl-sulfate-polyacrylamide gel-electrophoresis with protein and glycoprotein staining...
March 15, 2018: Food & Function
Zehang Zhou, Weerapha Panatdasirisuk, Tyler S Mathis, Babak Anasori, Canhui Lu, Xinxing Zhang, Zhiwei Liao, Yury Gogotsi, Shu Yang
Free-standing, highly flexible and foldable supercapacitor electrodes were fabricated through the spray-coating assisted layer-by-layer assembly of Ti3 C2 Tx (MXene) nanoflakes together with multi-walled carbon nanotubes (MWCNTs) on electrospun polycaprolactone (PCL) fiber networks. The open structure of the PCL network and the use of MWCNTs as spacers not only limit the restacking of Ti3 C2 Tx flakes but also increase the accessible surface of the active materials, facilitating fast diffusion of electrolyte ions within the electrode...
March 15, 2018: Nanoscale
Qinjun Sun, Shaolong Zhou, Xiaolei Shi, Xiaochun Wang, Liyan Gao, Zhanfeng Li, Yuying Hao
CuO nanowires (NWs) with the diameters ranging from 130 to 275 nm have been successfully prepared by electrospinning and calcination technique, followed by a calcination process. Inverted planar heterojunction perovskite solar cells (PSCs) with the structure of ITO/CuO NWs/PEDOT:PSS/CH3NH3PbI3/PCBM/Bphen/Ag were designed, achieving a best power conversion efficiency (PCE) of 16.87% , which is 21% improvement compared with that of the control PSCs without CuO NWs. By the characterizations of optical microscope, X-ray diffraction (XRD) and scanning electron microscopy (SEM), it is found that CuO NWs have uniform morphology and orderly arrangement...
March 15, 2018: ACS Applied Materials & Interfaces
Wen Shuo Chen, Ling Yu Guo, Chia Chun Tang, Cheng Kang Tsai, Hui Hua Huang, Ting Yu Chin, Mong-Lin Yang, Yui Whei Chen-Yang
In this study, we first synthesized a slow-degrading silica nanofiber (SNF2) through an electrospun solution with an optimized tetraethyl orthosilicate (TEOS) to polyvinyl pyrrolidone (PVP) ratio. Then, laminin-modified SNF2, namely SNF2-AP-S-L, was obtained through a series of chemical reactions to attach the extracellular matrix protein, laminin, to its surface. The SNF2-AP-S-L substrate was characterized by a combination of scanning electron microscopy (SEM), Fourier transform-infrared (FTIR) spectroscopy, nitrogen adsorption/desorption isotherms, and contact angle measurements...
March 14, 2018: Nanomaterials
Chaoyu Liu, Chong Wang, Qilong Zhao, Xiaohua Li, Feiyue Xu, Xumei Yao, Min Wang
Electrospun fibrous scaffolds have been extensively used as cell-supporting matrices or delivery vehicles for various biomolecules in tissue engineering. Biodegradable scaffolds with tunable degradation behaviors are favorable for various resorbable tissue replacements. In nerve tissue engineering, delivery of growth factors (GFs) such as nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) from scaffolds can be used to promote peripheral nerve repair. In this study, using the established dual-source dual-power (DS-DP) electrospinning technique, bicomponent scaffolds incorporated with NGF and GDNF were designed and demonstrated as a strategy to develop scaffolds providing dual GF delivery...
March 14, 2018: Biomedical Materials
Ripon Sarkar, Aritri Ghosh, Ananya Barui, Pallab Datta
Topical application of honey for tissue regeneration, has recently regained attention in clinical practice with controlled studies affirming its efficacy and indicating its role in regeneration over repair. Parallely, to overcome difficulties of applying raw honey, several product development studies like nanofibrous matrices have been reported. However, one approach concentrated on achieving highest possible honey loading in the nanofiber membranes while other studies have found that only specific honey dilutions result in differential cellular responses on wound healing and re-epithelization...
March 13, 2018: Journal of Materials Science. Materials in Medicine
Chengpeng Chen, Alexandra D Townsend, Elizabeth A Hayter, Hannah M Birk, Scott A Sell, R Scott Martin
We present an insert-based approach to fabricate scalable and multiplexable microfluidic devices for 3D cell culture and integration with downstream detection modules. Laser-cut inserts with a layer of electrospun fibers are used as a scaffold for 3D cell culture, with the inserts being easily assembled in a 3D-printed fluidic device for flow-based studies. With this approach, the number and types of cells (on the inserts) in one fluidic device can be customized. Moreover, after an investigation (i.e., stimulation) under flowing conditions, the cell-laden inserts can be removed easily for subsequent studies including imaging and cell lysis...
March 14, 2018: Analytical and Bioanalytical Chemistry
Roberto Scaffaro, Francesco Lopresti, Manuela D'Arrigo, Andreana Marino, Antonia Nostro
Carvacrol (CAR) is one of the most promising essential oil components with antimicrobial activity. New technologies aimed to incorporate this active molecule into carrier matrix to improve the stability and prolong the biological activity. The goal of this study was to investigate the feasibility of incorporating CAR into electrospun membranes of poly(lactic acid) (PLA) for potential applications as active antimicrobial system. To this end, PLA membranes containing homogeneously dispersed CAR were successfully prepared and a series of systematic tests including morpho-mechanical properties, in vitro release rate, and antimicrobial/antibiofilm activities against Staphylococcus aureus and Candida albicans were carried out...
March 13, 2018: Applied Microbiology and Biotechnology
Parmiss Mojir Shaibani, Hashem Rajab Etayash, Keren Jiang, Amirreza Sohrabi, Mahtab Hassanpourfard, Selvaraj Naicker, Mohtada Sadrzadeh, Thomas Thundat
The growing need to prevent pathogen outbreaks is irrefutable in the case of the food industry. Early detection in products, especially beverages, contaminated with bacterial strains is vital to avoid infected foods from reaching the consumer. If E. coli is pesent in such foods, it can cause infections. It can also be an indicator of the existence of other harmful coliforms. In this study, we have investigated the detection of Escherichia coli (E. coli) in orange juice using a portable nanofiber-light addressable potentiometric sensor (NF-LAPS)...
March 13, 2018: ACS Sensors
Angelina D Schoenenberger, Jasper Foolen, Pascal Moor, Unai Silvan, Jess G Snedeker
Healthy tendon tissue features a highly aligned extracellular matrix that becomes disorganized with disease. Recent evidence suggests that inflammation coexists with early degenerative changes in tendon, and that crosstalk between immune-cells and tendon fibroblasts (TFs) can contribute to poor tissue healing. We hypothesized that a disorganized tissue architecture may predispose tendon cells to degenerative extracellular matrix remodeling pathways, particularly within a pro-inflammatory niche. This hypothesis was tested by analyzing human TFs cultured on electrospun polycaprolactone (PCL) mats with either highly aligned or randomly oriented fiber structures...
March 9, 2018: Acta Biomaterialia
Mazaher Gholipourmalekabadi, Alexander M Seifalian, Aleksandra M Urbanska, Mir Davood Omrani, John G Hardy, Zahra Madjd, Seyed Mahmoud Hashemi, Hossein Ghanbarian, Peiman Brouki Milan, Masoud Mozafari, Rui L Reis, Subhas C Kundu, Ali Samadikuchaksaraei
Severe burn injuries can lead to delay in healing and devastating scar formation. Attempts are made to develop a suitable skin substitute for scarless healing of such skin wounds. Currently, there is no effective strategy yet for a complete scarless healing after the thermal injuries. In our recent work we fabricate and evaluated a 3D protein-based artificial skin made from decellularized human amniotic membrane (AM) and electrospun nanofibrous silk fibroin (ESF) in vitro. We also characterize both biophysical and cell culture investigation to establish in vitro performance of the developed bilayer scaffolds...
March 12, 2018: Biomacromolecules
Dries J G Devlaminck, Md Mahbubor Rahman, Mamoni Dash, Sangram Keshari Samal, Jan Watté, Sandra Van Vlierberghe, Peter Dubruel
HYPOTHESIS: The complete removal of remaining polymer debris after stripping of optical fiber cables is essential for high precision connection between two fibers. It can be anticipated that electrospun porous membranes as cleaning wipes are able to trap and retain polymer debris within their pores. Impregnation of an oil-in-water emulsion as cleaning agent lowers the interfacial tension between debris and the optical fiber thereby enabling the straightforward removal of polymer debris from the optical fiber...
February 13, 2018: Journal of Colloid and Interface Science
Nicole Jedrusik, Christoph Meyen, Günter Finkenzeller, G Björn Stark, Stephan Meskath, Simon Daniel Schulz, Thorsten Steinberg, Philipp Eberwein, Sandra Strassburg, Pascal Tomakidi
Regarding tissue regeneration, mechanics of biomaterials gains progressive importance. Therefore, this study reports on in situ crosslinked electrospun gelatin nonwoven mats (NWMs) whose distinct modulus of elasticity (ME) promotes epithelial tissue formation in a graded manner. NWMs, comprising fiber diameters in various distributions, yield an ME of about 2.1, 3.2, and 10.9 kPa. A two-step approach of preclinical in vitro validation identifies the elasticity of 3.2 kPa as superior to the other, regarding the histogenetic epithelial outcome...
March 12, 2018: Advanced Healthcare Materials
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