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https://www.readbyqxmd.com/read/28228626/osteogenic-induction-of-bone-marrow-mesenchymal-cells-on-electrospun-polycaprolactone-chitosan-nanofibrous-membrane
#1
Ying He, Wei Wang, Xuyan Tang, Xin Liu
A novel chitosan/polycaprolactone (CS/PCL) nanofibrous membrane by electrospinning was developed for guided tissue regeneration (GTR) to improve mechanical properties and to promote osteogenic differentiation. Firstly, chitosan and PCL solutions of different weight ratios (0/100, 30/70, 50/50) were mixed and then electrospun. Our data demonstrated that the CS/PCL (30/70) nanofibrous membrane promoted an increased rBMSCs proliferation when compared to the CS/PCL (50/50) membrane and pure PCL (0/100) membrane...
February 22, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28227434/electrospinning-of-gelatin-and-smpu-with-carbon-nanotubes-for-tissue-engineering-scaffolds
#2
Monica A Mejia, Lina M Hoyos, Jenniffer Zapata, Luz M Restrepo, Maria E Moneada, Monica A Mejia, Lina M Hoyos, Jenniffer Zapata, Luz M Restrepo, Maria E Moneada, Lina M Hoyos, Jenniffer Zapata, Monica A Mejia, Luz M Restrepo
The nanofibres created by electrospinning technique are currently used for a variety of applications in tissue engineering; and Gelatin and Polyurethane Shape-Memory (SMPU) have important results in biomedicine. Similarly, carbon nanotubes combined with other biomaterials change important properties, opening new opportunities for biomedical applications. In this work, we constructed scaffold using electrospinning technique based in bovine-hide gelatin, SMPU and both materials hybrid with carbon nanotube. Morphology and cytotoxicity were evaluated and mechanical properties for two materials were obtained in scaffold building...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226454/optimization-of-electrospun-fibrous-membranes-for-in-vitro-modeling-of-blood-brain-barrier
#3
V Pensabene, S W Crowder, D A Balikov, J B Lee, H J Sung, V Pensabene, S W Crowder, D A Balikov, J B Lee, H J Sung, V Pensabene, D A Balikov, S W Crowder, J B Lee, H J Sung
The blood-brain barrier (BBB) plays a critical role in brain homeostasis at the cellular and global level. Mimicking the selective permeability and transport properties of the BBB to specific molecules and cells remains a significant challenge towards the development of a physiologically relevant in vitro BBB model. In this study, we developed electrospun poly (ε-caprolactone) (PCL) and polyethylene glycol (PEG) copolymer membranes that supported different cellular components of the neurovascular unit including human-derived endothelial cells, pericytes and astrocytes...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225585/two-step-process-to-create-roll-off-superamphiphobic-paper-surfaces
#4
Lu Jiang, Zhenguan Tang, Rahmat M Clinton, Victor Breedveld, Dennis W Hess
Surface modification of cellulose-based paper which displays roll-off properties for water and oils (surface tension ≥ 23.8 mN·m-1) and good repellency towards n-heptane (20.1 mN·m-1) is reported. Droplets of water, diiodomethane, motor oil, hexadecane and decane all "bead up", i.e. exhibit high contact angles, and roll off the treated surface under the influence of gravity. Unlike widely used approaches that rely on the deposition of nanoparticles or electrospun nanofibers to create superamphiphobic surfaces, our method generates a hierarchical structure as an inherent property of the substrate and displays good adhesion between the film and substrate...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28225354/electrospun-assembly-a-nondestructive-nanofabrication-for-transparent-photosensors
#5
Yuting Wang, Muhammad Shahid, Jing Cheng, Hiroki Nishijima, Wei Pan
Transparent electrodes based on a metal nanotrough network show superior electrical and optical properties. However, most metal networks fabricated by electrospinning are formed as film electrodes and hard to be patterned for the geometry shape of the device without any loss. Herein, we fabricate a high-transparent and flexible photodetector via a simple controlled electrospinning method. Owning to its trough- and belt-like geometry of Pt network electrodes, up to 83% transmittance can be obtained when the sheet resistances is 16Ω/sq, which may be the best performance for Pt-based transparent electrodes at present...
February 22, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28225101/macrophage-infiltration-of-electrospun-polyester-fibers
#6
Shen Liu, Hua Chen, Tianyi Wu, Guoqing Pan, Cunyi Fan, Yaozeng Xu, Wenguo Cui
Electrospun fibrous polylactide (PLA) membranes have been widely used for peritendinous anti-adhesion, but their degradation may lead to granuloma formation triggered by macrophages as the result of a foreign body reaction. To understand and solve this problem in peritendinous anti-adhesion, the effects of electrospun fibrous PLA membranes (PLA-M) and the mechanism by which macrophages elicit a foreign body reaction should be elaborated. Thus, the purpose of this study was to evaluate the ability of PLA-M and ibuprofen (IBU)-loaded fibrous PLA membranes (IBU/PLA-M) to prevent adhesion/granuloma formation around the tendon, and to understand the mechanism of macrophage infiltration...
February 22, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28224971/biofabrication-of-bundles-of-poly-lactic-acid-collagen-blends-mimicking-the-fascicles-of-the-human-achille-tendon
#7
Alberto Sensini, Chiara Gualandi, Luca Cristofolini, Gianluca Tozzi, Manuela Dicarlo, Gabriella Teti, Monica Mattioli-Belmonte, Maria Letizia Focarete
Electrospinning is a promising technique for the production of scaffolds aimed at the regeneration of soft tissues. The aim of this work was to develop electrospun bundles mimicking the architecture and mechanical properties of the fascicles of the human Achille tendon. Two different blends of poly(L-lactic acid) (PLLA) and collagen (Coll) were tested, PLLA/Coll-75/25 and PLLA/Coll-50/50, and compared with bundles of pure PLLA. First, a complete physico-chemical characterization was performed on non-woven mats made of randomly arranged fibers...
February 22, 2017: Biofabrication
https://www.readbyqxmd.com/read/28224898/non-covalently-crosslinked-chitosan-nanofibrous-mats-prepared-by-electrospinning-as-substrates-for-soft-tissue-regeneration
#8
Chiara Tonda-Turo, Francesca Ruini, Martina Ramella, Francesca Boccafoschi, Piergiorgio Gentile, Emilia Gioffredi, Giuseppe Falvo D'Urso Labate, Gianluca Ciardelli
Chitosan (CS) membranes obtained by electrospinning are potentially ideal substrates for soft tissue engineering as they combine the excellent biological properties of CS with the extracellular matrix (ECM)-like structure of nanofibrous mats. However, the high amount of acid solvents required to spun CS solutions interferes with the biocompatibility of CS fibres. To overcome this limitation, novel CS based solutions were investigated in this work. Low amount of acidic acid (0.5M) was used and dibasic sodium phosphate (DSP) was introduced as ionic crosslinker to improve nanofibres water stability and to neutralize the acidic pH of electrospun membranes after fibres soaking in biological fluids...
April 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28223803/protein-nanocoatings-on-synthetic-polymeric-nanofibrous-membranes-designed-as-carriers-for-skin-cells
#9
Marketa Bacakova, Julia Pajorova, Denisa Stranska, Daniel Hadraba, Frantisek Lopot, Tomas Riedel, Eduard Brynda, Margit Zaloudkova, Lucie Bacakova
Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28222612/in-vitro-ex-vivo-correlations-between-a-cell-laden-hydrogel-and-mucosal-tissue-for-screening-composite-delivery-systems
#10
Anna K Blakney, Adam B Little, Yonghou Jiang, Kim A Woodrow
Composite delivery systems where drugs are electrospun in different layers and vary the drug stacking-order are posited to affect bioavailability. We evaluated how the formulation characteristics of both burst- and sustained-release electrospun fibers containing three physicochemically diverse drugs: dapivirine (DPV), maraviroc (MVC) and tenofovir (TFV) affect in vitro and ex vivo release. We developed a poly(hydroxyethyl methacrylate) (pHEMA) hydrogel release platform for the rapid, inexpensive in vitro evaluation of burst- and sustained-release topical or dermal drug delivery systems with varying microarchitecture...
November 2016: Drug Delivery
https://www.readbyqxmd.com/read/28221758/synthesis-and-fabrication-of-collagen-coated-ostholamide-electrospun-nanofiber-scaffold-for-wound-healing
#11
Subramani Kandhasamy, Sathiamurthi Perumal, Balaraman Madhan, Narayanan Umamaheswari, Javaid Ahmad Banday, Paramasivan Thirumalai Perumal, Vichangal Pridiuldi Santhanakrishnan
A novel scaffold for effective wound healing treatment was developed utilizing natural product bearing collagen based biocompatible electro-spun nanofibres. Initially, ostholamide (OSA) was synthesized from osthole (a natural coumarin), characterized by 1H, 13C, DEPT-135 NMR, ESI-MS and FT-IR spectroscopy analysis. OSA was incorporated into Polyhydroxybutyrate (PHB) and Gelatin (GEL), which serve as templates for electro-spun nanofibers. The coating of OSA-PHB-GEL nanofibers with collagen resulted in PHB-GEL-OSA-COL nanofibrous scaffold which mimics extra cellular matrix and serves as an effective biomaterial for tissue engineering applications, especially for wound healing...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28221164/positive-and-negative-cues-for-modulating-neurite-dynamics-and-receptor-expression
#12
Melissa Wrobel, Harini Gayathari Sundararaghavan
Many current peripheral nerve repair strategies focus on delivering positive, growth promoting cues (e.g. extracellular matrix, ECM) while eliminating negative, growth inhibiting cues (e.g. chondroitin sulfate proteoglycans, CSPGs) at the injury site. We hypothesized that recapitulating the positive and negative cues of the peripheral nerve injury microenvironment would improve regeneration. First, we tested the effects of a characteristic CSPG, Chondroitin Sulfate A (CSA) on neurite dynamics of dissociated chick embryo dorsal root ganglion (DRG) neurons using time lapse video microscopy...
February 21, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28216559/aloe-vera-for-tissue-engineering-applications
#13
REVIEW
Shekh Rahman, Princeton Carter, Narayan Bhattarai
Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components...
February 14, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28216468/enhanced-ocular-efficacy-of-topically-delivered-dorzolamide-with-nanostructured-mucoadhesive-microparticles
#14
Chun Gwon Park, Young Kook Kim, Se-Na Kim, Seung Ho Lee, Beom Kang Huh, Min-A Park, Hyein Won, Ki Ho Park, Young Bin Choy
Dorzolamide eye drops are widely prescribed to reduce intraocular pressure (IOP) in the treatment of ocular hypertension and glaucoma. However, in an eye drop formulation, dorzolamide is rapidly cleared from the preocular space, hence requiring multiple daily administrations. Here, we sought to increase the preocular retention of dorzolamide using nanostructured, mucoadhesive microparticles (MUCO_NM) as carriers for topical delivery to the eye. MUCO_NM were prepared by freeze-milling dorzolamide-loaded, electrospun nanofibers composed of poly(lactic-co-glycolic acid) and polyethylene glycol...
February 16, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28216302/long-term-antimicrobial-effect-of-nisin-released-from-electrospun-triaxial-fiber-membranes
#15
Daewoo Han, Shalli Sherman, Shaun Filocamo, Andrew J Steckl
: Electrospun membranes encapsulating nisin in the core of multi-layer coaxial fibers, with a hydrophobic PCL intermediate layer and a hygroscopic cellulose acetate sheath, have been demonstrated to provide long-term antimicrobial activity combined with a hygroscopic outer layer. Antimicrobial performance has been evaluated using modified versions of the antimicrobial textile test AATCC 100 and AATCC 147 against Staphylococcus aureus. The AATCC 147 tests indicate that antimicrobial activity persists up to 7 days...
February 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28214952/novel-potential-scaffold-for-periodontal-tissue-engineering
#16
Raquel Osorio, Camilo Andrés Alfonso-Rodríguez, Estrella Osorio, Antonio L Medina-Castillo, Miguel Alaminos, Manuel Toledano-Osorio, Manuel Toledano
OBJECTIVE: The objective of the study is characterization of novel calcium and zinc-loaded electrospun matrices to be used for periodontal regeneration. MATERIALS AND METHODS: A polymethylmetacrylate-based membrane was calcium or zinc loaded. Matrices were characterized morphologically by atomic force and scanning electron microscopy and mechanically probed by a nanoindenter. Biomimetic calcium phosphate precipitation on polymeric tissues was assessed. Cell viability tests were performed using oral mucosa fibroblasts...
February 18, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28211362/marrow-isolated-adult-multilineage-inducible-cells-embedded-within-a-biologically-inspired-construct-promote-recovery-in-a-mouse-model-of-peripheral-vascular-disease
#17
Cristina Grau-Monge, Gaëtan J-R Delcroix, Andrea Bonnin-Marquez, Mike Valdes, Ead Lewis Mazen Awadallah, Daniel F Quevedo, Maxime R Armour, Ramon B Montero, Paul C Schiller, Fotios M Andreopoulos, Gianluca D'Ippolito
Peripheral vascular disease is one of the major vascular complications in individuals suffering from diabetes and in the elderly that is associated with significant burden in terms of morbidity and mortality. Stem cell therapy is being tested as an attractive alternative to traditional surgery to prevent and treat this disorder. The goal of this study was to enhance the protective and reparative potential of marrow-isolated adult multilineage inducible (MIAMI) cells by incorporating them within a bio-inspired construct (BIC) made of two layers of gelatin B electrospun nanofibers...
February 17, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28211272/effects-of-dimethyloxalylglycine-embedded-poly-%C3%AE%C2%B5-caprolactone-fiber-meshes-on-wound-healing-in-diabetic-rats
#18
Qiankun Zhang, Joung-Hwan Oh, Chan Ho Park, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Impaired wound healing in diabetic patients is associated with altered inflammatory responses, poor angiogenesis, deficient extracellular matrix (ECM) component, and peripheral neuropathy. To develop a wound dressing that is capable of the controlled delivery of bioactive small molecules that can improve diabetic wound healing, dimethyloxalylglycine (DMOG)-embedded poly(ε-caprolactone) (PCL) fiber (PCLF/DMOG) meshes are fabricated by electrospinning, and the effects of the PCLF/DMOG meshes on wound healing in diabetic rats are evaluated...
February 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28209035/electrospun-perovskite-nanofibers
#19
Dongsheng Chen, Yanyan Zhu
CH3NH3PbI3 perovskite nanofibers were synthesized by versatile electrospinning techniques. The synthetic CH3NH3PbI3 nanofibers were characterized by X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, and photoluminescence. As counter electrodes, the synthesized nanofibers increased the performance of the dye-sensitized solar cells from 1.58 to 2.09%. This improvement was attributed to the enhanced smoothness and efficiency of the electron transport path. Thus, CH3NH3PbI3 perovskites nanofibers are potential alternative to platinum counter electrodes in dye-sensitized solar cells...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28209034/production-of-conductive-pedot-coated-pva-go-composite-nanofibers
#20
Nur Afifah Zubair, Norizah Abdul Rahman, Hong Ngee Lim, Yusran Sulaiman
Electrically conductive nanofiber is well known as an excellent nanostructured material for its outstanding performances. In this work, poly(3,4-ethylenedioxythiophene) (PEDOT)-coated polyvinyl alcohol-graphene oxide (PVA-GO)-conducting nanofibers were fabricated via a combined method using electrospinning and electropolymerization techniques. During electrospinning, the concentration of PVA-GO solution and the applied voltage were deliberately altered in order to determine the optimized electrospinning conditions...
December 2017: Nanoscale Research Letters
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