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https://www.readbyqxmd.com/read/29439248/transient-receptor-potential-vanilloid-4-activation-induced-increase-in-glycine-activated-current-in-mouse-hippocampal-pyramidal-neurons
#1
Mengwen Qi, Chunfeng Wu, Zhouqing Wang, Li Zhou, Chen Men, Yimei Du, Songming Huang, Lei Chen, Ling Chen
BACKGROUND/AIMS: Glycine plays an important role in regulating hippocampal inhibitory/ excitatory neurotransmission through activating glycine receptors (GlyRs) and acting as a co-agonist of N-methyl-d-aspartate-type glutamate receptors. Activation of transient receptor potential vanilloid 4 (TRPV4) is reported to inhibit hippocampal A-type γ-aminobutyric acid receptor, a ligand-gated chloride ion channel. GlyRs are also ligand-gated chloride ion channels and this paper aimed to explore whether activation of TRPV4 could modulate GlyRs...
February 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29436688/necrostatin-1-protects-c2c12-myotubes-from-cocl2-induced-hypoxia
#2
Rui Chen, Jiehua Xu, Yanling She, Ting Jiang, Shanyao Zhou, Huacai Shi, Cheng Li
Necrostatin-1 (Nec-1) is a selective and potent allosteric inhibitor of necroptosis by specifically inhibiting the activity of receptor‑interacting protein (RIP) 1 kinase. The aim of the present study was to determine the effect of Nec‑1 on an anoxia model comprising mouse skeletal C2C12 myotubes. In the present study, a hypoxic mimetic reagent, cobalt chloride (CoCl2), was used to induce hypoxia in C2C12 myotubes. The cytotoxic effects of CoCl2‑induced hypoxia were determined by a Cell Counting kit‑8 assay and flow cytometry...
February 6, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29436118/cortistatin-inhibits-arterial-calcification-in-rats-via-gsk3%C3%AE-%C3%AE-catenin-and-pkc-signaling-but-not-jnk-signaling
#3
Yue Liu, Fang Lin, Yu Fu, Wenjia Chen, Wenxiu Liu, Jinyu Chi, Xiaohui Zhang, Xinhua Yin
AIM: Cortistatin (CST) is a newly discovered endogenous active peptide that exerts protective effects on the cardiovascular system. However, the relationship between CST and aortic calcification and the underlying mechanism remain obscure. Therefore, we investigated effects of CST on aortic calcification and its signaling pathways. METHODS: Calcium content and alkaline phosphatase (ALP) activity were measured using the o-cresolphthalein colorimetric method and ALP assay kit, respectively...
February 13, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29421646/protective-effects-of-dietary-luteolin-against-mercuric-chloride-induced-lung-injury-in-mice-involvement-of-akt-nrf2-and-nf-%C3%AE%C2%BAb-pathways
#4
Biying Liu, Hongxiang Yu, Ruiqi Baiyun, Jingjing Lu, Siyu Li, Qizheng Bing, Xiaoya Zhang, Zhigang Zhang
Food-derived compound luteolin possesses multiple pharmacological activities. Accordingly, we focused on exploring the protective effects of luteolin (100 mg/kg) against mercuric chloride (HgCl2) (5 mg/kg) stimulated lung injury and the molecular mechanisms of lung protection effects in mouse. The influence of luteolin on histologic changes, oxidative stress, proinflammatory cytokine production, neutrophil activation, and apoptosis were assayed in HgCl2-induced lung injury. Luteolin administration attenuated pulmonary histologic conditions and apoptotic change...
February 5, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29413957/2-chlorohexadecanoic-acid-induces-er-stress-and-mitochondrial-dysfunction-in-brain-microvascular-endothelial-cells
#5
Eva Bernhart, Nora Kogelnik, Jürgen Prasch, Benjamin Gottschalk, Madeleine Goeritzer, Maria Rosa Depaoli, Helga Reicher, Christoph Nusshold, Ioanna Plastira, Astrid Hammer, Günter Fauler, Roland Malli, Wolfgang F Graier, Ernst Malle, Wolfgang Sattler
Peripheral leukocytes induce blood-brain barrier (BBB) dysfunction through the release of cytotoxic mediators. These include hypochlorous acid (HOCl) that is formed via the myeloperoxidase-H2O2-chloride system of activated phagocytes. HOCl targets the endogenous pool of ether phospholipids (plasmalogens) generating chlorinated inflammatory mediators like e.g. 2-chlorohexadecanal and its conversion product 2-chlorohexadecanoic acid (2-ClHA). In the cerebrovasculature these compounds inflict damage to brain microvascular endothelial cells (BMVEC) that form the morphological basis of the BBB...
January 5, 2018: Redox Biology
https://www.readbyqxmd.com/read/29412704/with-no-lysine-kinase-4-modulates-sodium-potassium-2-chloride-cotransporter-activity-in-vivo
#6
Andrew Scott Terker, Maria Castaneda-Bueno, Mohammed Zubaerul Ferdaus, Ryan J Cornelius, Kayla J Erspamer, Xiao-Tong Su, Lauren N Miller, James A McCormick, Wen-Hui Wang, Gerardo Gamba, Chao-Ling Yang, David H Ellison
With no lysine kinase 4 (WNK4) is essential to activate the thiazide-sensitive NaCl cotransporter (NCC) along the distal convoluted tubule, an effect central to the phenotype of familial hyperkalemic hypertension. Although effects on potassium and sodium channels along the connecting and collecting tubules have also been documented, WNK4 is typically believed to have little role in modulating sodium chloride reabsorption along the thick ascending limb of the loop of Henle. Yet wnk4-/- mice (knockout mice lacking WNK4) do not demonstrate the hypocalciuria typical of pure distal convoluted tubule dysfunction...
February 7, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29410165/mid-gestational-sevoflurane-exposure-inhibits-fetal-neural-stem-cell-proliferation-and-impairs-postnatal-learning-and-memory-function-in-a-dose-dependent-manner
#7
Yuan Wang, Shaowei Yin, Hang Xue, Yating Yang, Nan Zhang, Ping Zhao
Advancements in fetal intervention procedures have led to increases in the number of pregnant women undergoing general anesthesia during the second trimester-a period characterized by extensive proliferation of fetal neural stem cells (NSCs). However, few studies have investigated the effects of mid-gestational sevoflurane exposure on fetal NSC proliferation or postnatal learning and memory function. In the present study, pregnant rats were randomly assigned to a control group (C group), a low sevoflurane concentration group (2%; L group), a high sevoflurane concentration group (3...
January 31, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29390191/inducible-knockdown-of-endothelial-protein-tyrosine-phosphatase-1b-promotes-neointima-formation-in-obese-mice-by-enhancing-endothelial-senescence
#8
Marianne Jäger, Astrid Hubert, Rajinikanth Gogiraju, Magdalena L Bochenek, Thomas Münzel, Katrin Schäfer
AIMS: Protein tyrosine phosphatase-1B (PTP1B) is a negative regulator of receptor tyrosine kinase signaling. In this study, we determined the importance of PTP1B expressed in endothelial cells for the vascular response to arterial injury in obesity. RESULTS: Morphometric analysis of vascular lesions generated by 10% ferric chloride (FeCl3) revealed that tamoxifen-inducible endothelial PTP1B deletion (Tie2.ERT2-Cre × PTP1Bfl/fl; End.PTP1B knockout, KO) significantly increased neointima formation, and reduced numbers of (endothelial lectin-positive) luminal cells in End...
February 1, 2018: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29383422/proteomic-alterations-of-brain-subcellular-organelles-caused-by-low-dose-copper-exposure-implication-for-alzheimer-s-disease
#9
Haitao Yu, Dian Wang, Liangyu Zou, Zaijun Zhang, Hua Xu, Feiqi Zhu, Xiaohu Ren, Benhong Xu, Jianhui Yuan, Jianjun Liu, Peter S Spencer, Xifei Yang
Excessive copper intake can lead to neurotoxicity, but there is a lack of comprehensive understanding on the potential impact of copper exposure especially at a low-dose on brain. We used 3xTg-AD mice to explore the potential neurotoxicity of chronic, low-dose copper treatment (0.13 ppm copper chloride in drinking water) on behavior and the brain hippocampal mitochondrial and nuclear proteome. Low-dose copper increased the spatial memory impairment of these animals, increased accumulation of intracellular amyloid 1-42 (Aβ1-42), decreased ATP content, increased the positive staining of 8-hydroxyguanosine (8-OHdG), a marker of DNA oxidative damage, and caused apoptosis and a decrease in synaptic proteins...
January 30, 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/29382423/-huangjiao-granules-ameliorate-brain-injury-in-rats-with-cerebral-ischemia-reperfusion-injury-by-stimulating-pi3k-akt-mtor-signaling-pathway
#10
Songbin Pan, Lin Wan, Wei Shao, Kun Tang, Hanyun Yao
Objective To investigate the protective effect of Huangjiao granules on rats with cerebral ischemia/reperfusion injury and the effect on phosphoinositide-3 kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway. Methods The rat models of cerebral ischemia/reperfusion injury were established by suture method and treated with Huangjiao granules. ZeaLonga scoring was used to evaluate the neurological function of rats. The percentage of cerebral infarction was detected by triphenyl tetrazolium chloride (TTC) staining...
December 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29372915/removal-of-the-fe-iii-site-promotes-activation-of-the-human-cystic-fibrosis-transmembrane-conductance-regulator-by-high-affinity-zn-ii-binding
#11
Guangyu Wang
The cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel is activated by ATP binding at the interface of two cytoplasmic nucleotide binding domains (NBDs) and phosphorylation of the regulatory (R) domain by protein kinase A (PKA). The human CFTR has two functionally active thiol groups for gating regulation by chemical modification. Although modification of C832 in the R domain with N-ethylmaleimide promotes channel opening, glutathionylation of C1344 in NBD2 inhibits channel opening...
January 26, 2018: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29357415/interfering-rna-against-pkc-%C3%AE-inhibits-tnf-%C3%AE-induced-ip3r1-expression-and-improves-glomerular-filtration-rate-in-rats-with-fulminant-hepatic-failure
#12
Dong-Lei Wang, Wen-Ying Dai, Wen Wang, Ying Wen, Ying Zhou, Yi-Tong Zhao, Jian Wu, Pei Liu
BACKGROUND & AIMS: We have reported that tumor necrosis factor- (TNF-α) is critical for reduction of glomerular filtration rate (GFR) in rats with fulminant hepatic failure (FHF). The present study aims to evaluate the underlying mechanisms of decreased GFR during acute hepatic failure. METHODS: Rats with FHF induced by D-galactosamine plus lipopolysaccharide (GalN/LPS) were injected intravenously with recombinant lentivirus harboring shRNA against the protein kinase C-α (PKC-α) gene (Lenti-shRNA-PKC-α)...
January 10, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29354919/-effects-of-electroacupuncture-at-weizhong-bl-40-on-morphology-and-expression-of-ck-and-il-17-in-rats-with-bupivacaine-induced-multifidus-muscle-injury
#13
Dehui Zou, Yupei Chen, Tong Liu, Zongxiao Lu, Jun Yan, Dongli Chen, Yue Xu, Jiayi Zhang, Yuzhuo Bai, Li Zhang, Zejun Huo
OBJECTIVE: To observe effects of electroacupuncture (EA) at "Weizhong" (BL 40) on morphology and expression of creatine kinase (CK) and interleukin-17 (IL-17) in rats with bupivacaine-induced multifidus muscle injury. METHODS: A total of 32 male SD rats were randomly divided into a control group, a model group, a Weizhong group and a Shenshu group, 8 rats in each one. The rats in the model group, Weizhong group and Shenshu group were treated with intramuscular injection of 0...
September 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29351415/amp-activated-protein-kinase-and-adenosine-are-both-metabolic-modulators-regulating-chloride-secretion-in-the-shark-rectal-gland-squalus-acanthias
#14
Rugina I Neuman, Juliette A M van Kalmthout, Daniel J Pfau, Dhariyat M Menendez, Lawrence H Young, John N Forrest
The production of endogenous adenosine during secretagogue stimulation of CFTR leads to feedback inhibition limiting further chloride secretion in the rectal gland of the dogfish shark (Squalus acanthias). In the present study, we examined the role of AMP-kinase (AMPK) as an energy sensor also modulating chloride secretion through CFTR. We found that glands perfused with forskolin and isobutylmethylxanthine (F + I), potent stimulators of chloride secretion in this ancient model, caused significant phosphorylation of the catalytic subunit Thr172 of AMPK...
December 20, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29345351/k313-a-novel-benzoxazole-derivative-exhibits-anti-inflammatory-properties-via-inhibiting-gsk3%C3%AE-activity-in-lps-induced-raw264-7-macrophages
#15
Bo-Bo Zhao, Hui-Jie Guo, Yang Liu, Xing-Yan Luo, Shu-Xia Yang, Yan-Tang Wang, Xiao Leng, Chun-Fen Mo, Qiang Zou
Benzoxazole and its derivatives have been widely studied in recent years due to their various biological properties. A previous study has demonstrated that K313 (1H-indole-2,3-dione 3-(1,3-benzoxazol-2-ylhydrazone)), a novel benzoxazole derivative, inhibits T cell proliferation to yield immunosuppressive effects. However, there are no related reports about its anti-inflammatory effects. In the present study, we investigated the anti-inflammatory properties and the underlying molecular mechanism of K313 in lipopolysaccharide (LPS)-induced RAW264...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29329909/the-signaling-role-for-chloride-in-the-bidirectional-communication-between-neurons-and-astrocytes
#16
REVIEW
Corinne S Wilson, Alexander A Mongin
It is well known that the electrical signaling in neuronal networks is modulated by chloride (Cl-) fluxes via the inhibitory GABAA and glycine receptors. Here, we discuss the putative contribution of Cl- fluxes and intracellular Cl- to other forms of information transfer in the CNS, namely the bidirectional communication between neurons and astrocytes. The manuscript (i) summarizes the generic functions of Cl- in cellular physiology, (ii) recaps molecular identities and properties of Cl- transporters and channels in neurons and astrocytes, and (iii) analyzes emerging studies implicating Cl- in the modulation of neuroglial communication...
January 9, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29329019/salidroside-influences-the-cellular-cross-talk-of-human-fetal-lung-diploid-fibroblasts-a-proteomic-approach
#17
Wenmin Xing, Wenyan Gao, Huili Su, Sanying Wang, Jing Zhang, Genxiang Mao, Jing Yan
Senescence is a complex multiple factor proces, which is still poorly understood. The purpose of this study was to find the proteome of cultured human fetal lung diploid fibroblasts (2BS) of different population doubling (PD), as well as the altered proteome induced by salidroside (SAL) in 2BS cells. Proteins were identified by two-dimensional electrophoresis (2-DE) combining matrix-assisted laser desorption/ionization-time and flight mass spectrometry (MAL DI-TOF/MS). As a result, we found 16 proteins with two-fold variations in senescent cells or after SAL treatment, some being reduced such as reticulocalbin-1, heat shock protein beta-6, elongation factor 1-delta, F-actin-capping protein subunit alpha-1, and chloride intracellular channel 1...
January 5, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29328429/anthocyanins-inhibit-high-glucose-induced-renal-tubular-cell-apoptosis-caused-by-oxidative-stress-in-db-db-mice
#18
Jinying Wei, Haijiang Wu, Haiqiang Zhang, Fang Li, Shurui Chen, Baohua Hou, Yonghong Shi, Lijuan Zhao, Huijun Duan
Oxidative stress is an important contributory factor resulting the development of kidney injury in patients with diabetes. Numerous in vitro and in vivo studies have suggested that anthocyanins, natural phenols commonly existing in numerous fruits and vegetables, exhibit important antioxidative, anti‑inflammatory and antihyperlipidemic effects; however, their effects and underlying mechanisms on diabetic nephropathy (DN) have not yet been fully determined. In the present study, the regulation of apoptosis metabolism and antioxidative effects exhibited by anthocyanins [grape seed procyanidin (GSPE) and cyanidin‑3‑O‑β‑glucoside chloride (C3G)] were investigated, and the molecular mechanism underlying this process was investigated in vivo and in vitro...
January 10, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29326302/role-of-clc-k-and-barttin-in-low-potassium-induced-sodium-chloride-cotransporter-activation-and-hypertension-in-mouse-kidney
#19
Naohiro Nomura, Wakana Shoda, Yuanlong Wang, Shintaro Mandai, Taisuke Furusho, Daiei Takahashi, Moko Zeniya, Eisei Sohara, Tatemitsu Rai, Shinichi Uchida
The sodium-chloride cotransporter (NCC) has been identified as a key molecule regulating potassium balance. The mechanisms of NCC regulation during low extracellular potassium concentrations have been studied in vitro. These studies have shown that hyperpolarization increased chloride efflux, leading to the activation of chloride-sensitive WNK kinases and their downstream molecules, including STE20/SPS1-related proline/alanine-rich kinase (SPAK) and NCC. However, this mechanism was not studied in vivo Previously, we developed the barttin hypomorphic mouse ( Bsndneo/neo mice), expressing very low levels of barttin and ClC-K channels, because barttin is an essential ß-subunit of ClC-K...
January 11, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29325368/-the-effects-and-related-mechanism-of-salvianolate-on-rats-with-myocardial-ischemia-reperfusion-injury
#20
R C Yue, X L Yang, R Y Zhang, S Liu, J Liu, J Zeng, H Liang, W Wang, H X Hu, C Y Zeng
Objective: To investigate the effect and related mechanism of salvianolate on myocardial ischemia-reperfusion (I/R) injury in rats. Methods: Thirty-six adult Sprague-Dawley rats were divided into three groups (n=12 each) using random number table method: control group (coronary artery was not ligated) , I/R group (myocardial I/R model was established by ligation and opening of left anterior descending coronary artery) , and salvianolate+I/R group (5 mg/kg of salvianolate was injected through the tail vein at the time of reperfusion) ...
December 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
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