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https://www.readbyqxmd.com/read/28077321/camkii%C3%AE-subtypes-differentially-regulate-infarct-formation-following-ex-vivo-myocardial-ischemia-reperfusion-through-nf-%C3%AE%C2%BAb-and-tnf-%C3%AE
#1
Charles B B Gray, Takeshi Suetomi, Sunny Xiang, Erik A Blackwood, Christopher C Glembotski, Shigeki Miyamoto, B Daan Westenbrink, Joan Heller Brown
Deletion of Ca(2+)/calmodulin-dependent protein kinase II delta (CaMKIIδ) has been shown to protect against in vivo ischemia/reperfusion (I/R) injury. It remains unclear which CaMKIIδ isoforms and downstream mechanisms are responsible for the salutary effects of CaMKIIδ gene deletion. In this study we sought to compare the roles of the CaMKIIδB and CaMKIIδC subtypes and the mechanisms by which they contribute to ex vivo I/R damage. WT, CaMKIIδKO, and mice expressing only CaMKIIδB or δC were subjected to ex vivo global ischemia for 25min followed by reperfusion...
January 7, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28067240/cd8-t-cells-stimulate-na-cl-co-transporter-ncc-in-distal-convoluted-tubules-leading-to-salt-sensitive-hypertension
#2
Yunmeng Liu, Tonya M Rafferty, Sung W Rhee, Jessica S Webber, Li Song, Benjamin Ko, Robert S Hoover, Beixiang He, Shengyu Mu
Recent studies suggest a role for T lymphocytes in hypertension. However, whether T cells contribute to renal sodium retention and salt-sensitive hypertension is unknown. Here we demonstrate that T cells infiltrate into the kidney of salt-sensitive hypertensive animals. In particular, CD8(+) T cells directly contact the distal convoluted tubule (DCT) in the kidneys of DOCA-salt mice and CD8(+) T cell-injected mice, leading to up-regulation of the Na-Cl co-transporter NCC, p-NCC and the development of salt-sensitive hypertension...
January 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28060847/enhanced-chondrogenic-differentiation-of-human-umbilical-cord-wharton-s-jelly-derived-mesenchymal-stem-cells-by-gsk-3-inhibitors
#3
Prapot Tanthaisong, Sumeth Imsoonthornruksa, Apichart Ngernsoungnern, Piyada Ngernsoungnern, Mariena Ketudat-Cairns, Rangsun Parnpai
Articular cartilage is an avascular, alymphatic, and aneural system with very low regeneration potential because of its limited capacity for self-repair. Mesenchymal stem cells (MSCs) are the preferred choice for cell-based therapies. Glycogen synthase kinase 3 (GSK-3) inhibitors are compounds that can induce the Wnt signaling pathway, which is involved in chondrogenesis and cartilage development. Here, we investigated the influence of lithium chloride (LiCl) and SB216763 synergistically with TGF-β3 on chondrogenic differentiation in human mesenchymal stem cells derived from Wharton's jelly tissue (hWJ-MSCs)...
2017: PloS One
https://www.readbyqxmd.com/read/28056927/kudiezi-injection-mitigates-myocardial-injury-induced-by-acute-cerebral-ischemia-in-rats
#4
Xuemei Liu, Ye Tao, Fengli Wang, Ting Yao, Chen Fu, Hong Zheng, Yan Yan, Xiao Liang, Xiangning Jiang, Yunling Zhang
BACKGROUND: Kudiezi (KDZ) injection is commonly used in traditional Chinese medicine as treatment for cerebral infarction and angina pectoris. The present study investigated the therapeutic effects of KDZ injection on myocardial injury induced by acute cerebral ischemia and the possibly protective mechanisms. METHODS: Rats were divided into three groups: sham, 6h-ischemia, and KDZ treatment (KDZ). The neurological deficits were determined by the Garcia score. The cerebral infarct volume was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and brain water content was also evaluated...
January 5, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28012960/heme-oxygenase-ho-1-induction-prevents-endoplasmic-reticulum-stress-mediated-endothelial-cell-death-and-impaired-angiogenic-capacity
#5
Hatem Maamoun, Matshediso Zachariah, John H McVey, Fiona R Green, Abdelali Agouni
Most of diabetic cardiovascular complications are attributed to endothelial dysfunction and impaired angiogenesis. Endoplasmic Reticulum (ER) and oxidative stresses were shown to play a pivotal role in the development of endothelial dysfunction in diabetes. Hemeoxygenase-1 (HO-1) was shown to protect against oxidative stress in diabetes; however, its role in alleviating ER stress-induced endothelial dysfunction remains not fully elucidated. We aim here to test the protective role of HO-1 against high glucose-mediated ER stress and endothelial dysfunction and understand the underlying mechanisms with special emphasis on oxidative stress, inflammation and cell death...
December 21, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28011945/orexin-a-modulates-ins-1e-cell-proliferation-and-insulin-secretion-via-extracellular-signal-regulated-kinase-and-transient-receptor-potential-channels
#6
M Skrzypski, N Khajavi, S Mergler, M Billert, D Szczepankiewicz, T Wojciechowicz, K W Nowak, M Z Strowski
Orexins A (OXA) and B (OXB) control energy homeostasis by regulating food intake, energy expenditure and sleep-wake cycle. Several studies showed that OXA stimulates insulin secretion and proliferation of beta cells. However, mechanisms of action are still not well understood. Here, we investigated whether ERK and transient receptor potential channels (TRPs) play a role in mediating the effect of OXA on cell growth, insulin production, and secretion using the established INS-1E cell line. Cell proliferation was measured using BrdU assay...
October 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28011640/regulation-of-ulk1-expression-and-autophagy-by-stat1
#7
Alexander A Goldberg, Bernard Nkengfac, Anthony M J Sanchez, Nikolay Moroz, Salman T Qureshi, Antonis E Koromilas, Shuo Wang, Yan Burelle, Sabah N Hussain, Arnold S Kristof
Autophagy involves the lysosomal degradation of cytoplasmic contents for regeneration of anabolic substrates during nutritional or inflammatory stress. Its initiation occurs rapidly after inactivation of the protein- kinase 'mammalian target of rapamycin' (mTOR; or 'mechanistic target of rapamycin', MTOR), leading to de-phosphorylation of Unc-51-like kinase 1 (ULK1) and autophagosome formation. Recent studies indicate that mTOR can, in parallel, regulate the activity of stress transcription factors, including 'signal transducer and activator of transcription-1' (STAT1)...
December 23, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27999941/extracellular-phosphates-enhance-activities-of-voltage-gated-proton-channels-and-production-of-reactive-oxygen-species-in-murine-osteoclast-like-cells
#8
Guangshuai Li, Katsuyuki Miura, Miyuki Kuno
Osteoclasts are highly differentiated bone-resorbing cells and play a significant role in bone remodelling. In the resorption pit, inorganic phosphate (Pi) concentrations increase because of degradation of hydroxyapatite. We studied effects of extracellular Pi on voltage-gated H(+) channels in osteoclast-like cells derived from a macrophage cell line (RAW264). Extracellular Pi (1.25-20 mM) increased the H(+) channel currents dose dependently and reversibly. The Pi-induced increases were attenuated by removal of extracellular Na(+) and by phosphonoformic acid, a blocker of Na(+)-dependent Pi transporters...
December 21, 2016: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27998718/camkii-mediated-phosphorylation-of-glun2b-regulates-recombinant-nmda-receptor-currents-in-a-chloride-dependent-manner
#9
Steven J Tavalin, Roger J Colbran
Some forms of long-term synaptic plasticity require docking of Ca(2+)/calmodulin-dependent protein kinase II α (CaMKIIα) to residues 1290-1309 within the intracellular C-terminal tail of the N-methyl-d-aspartate (NMDA) receptor GluN2B subunit. The phosphorylation of Ser1303 within this region destabilizes CaMKII binding. Interestingly, Ser1303 is a substrate for CaMKII itself, as well as PKC and DAPK1, but these kinases have been reported to have contradictory effects on the activity of GluN2B-containing NMDA receptors...
December 18, 2016: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/27994435/pkr-promotes-choroidal-neovascularization-via-upregulating-the-pi3k-akt-signaling-pathway-in-vegf-expression
#10
Manhui Zhu, Xiaojuan Liu, Shengcun Wang, Jin Miao, Liucheng Wu, Xiaowei Yang, Ying Wang, Lihua Kang, Wendie Li, Chen Cui, Hui Chen, Aimin Sang
PURPOSE: The aim of this study was to investigate the functions of dsRNA-activated protein kinase (PKR) in choroidal neovascularization (CNV) and related signaling pathways in the production of vascular endothelial growth factor (VEGF). METHODS: A chemical hypoxia model of in vitro RF/6A cells, a rhesus choroid-retinal endothelial cell line, was established by adding cobalt chloride (CoCl2) to the culture medium. PKR, phosphophosphatidylinositol 3-kinase (p-PI3K), phosphoprotein kinase B (p-Akt), and VEGF protein levels in RF/6A cells were detected with western blotting...
2016: Molecular Vision
https://www.readbyqxmd.com/read/27991911/inhibition-of-infarction-induced-sympathetic-innervation-with-endothelin-receptor-antagonism-via-a-pi3k-gsk-3%C3%AE-dependent-pathway
#11
T-M Lee, Nen-Chung Chang, Shinn-Zong Lin
Although endothelin (ET)-1 has been shown to upregulate nerve growth factor (NGF) expression, the molecular mechanisms are largely unknown. Phosphatidylinositol 3-kinase (PI3K)/Akt/glycogen synthase kinase (GSK)-3β signal has been implicated in the regulation of NGF. We investigated whether selective ET receptor blockers attenuated cardiac sympathetic reinnervation through restoring PI3K/Akt/GSK-3β activity. After ligation of the left anterior descending artery, male Wistar rats were randomized to either vehicle, atrasentan (an ETA receptor antagonist) or A-192621 (an ETB receptor antagonist) for 4 weeks...
December 19, 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/27966879/single-molecule-fret-measurements-in-additive-enriched-aqueous-solutions
#12
Daryan Kempe, Michele Cerminara, Simón Poblete, Antonie Schöne, Matteo Gabba, Jörg Fitter
The addition of high amounts of chemical denaturants, salts, viscosity enhancers or macro-molecular crowding agents has an impact on the physical properties of buffer solutions. Among others, the (microscopic) viscosity, the refractive index, the dielectric constant, and the ionic strength can be affected. Here, we systematically evaluate the importance of solvent characteristics with respect to single-molecule FRET (smFRET) data. First, we present a confocal based method for the determination of fluorescence quantum yields to facilitate a fast characterization of smFRET-samples at sub-nM-concentrations...
December 14, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27965733/calcium-oxalate-induces-renal-injury-through-calcium-sensing-receptor
#13
Xiaoran Li, Junhai Ma, Wei Shi, Yu Su, Xu Fu, Yanlin Yang, Jianzhong Lu, Zhongjin Yue
Objective. To investigate whether calcium-sensing receptor (CaSR) plays a role in calcium-oxalate-induced renal injury. Materials and Methods. HK-2 cells and rats were treated with calcium oxalate (CaOx) crystals with or without pretreatment with the CaSR-specific agonist gadolinium chloride (GdCl3) or the CaSR-specific antagonist NPS2390. Changes in oxidative stress (OS) in HK-2 cells and rat kidneys were assessed. In addition, CaSR, extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal protein kinase (JNK), and p38 expression was determined...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27959381/nobiletin-a-citrus-flavonoid-activates-vasodilator-stimulated-phosphoprotein-in-human-platelets-through-non-cyclic-nucleotide-related-mechanisms
#14
Thanasekaran Jayakumar, Kao-Chang Lin, Wan-Jung Lu, Chia-Ying Lin, Geraldine Pitchairaj, Jiun-Yi Li, Joen-Rong Sheu
Nobiletin, a bioactive polymethoxylated flavone, has been described to possess a diversity of biological effects through its antioxidant and anti-inflammatory properties. Vasodilator-stimulated phosphoprotein (VASP) is a common substrate for cyclic AMP and cyclic GMP-regulated protein kinases [i.e., cyclic AMP-dependent protein kinase (PKA; also known as protein kinase A) and cyclic GMP-dependent protein kinase (PKG; also known as protein kinase G)] and it has been shown to be directly phosphorylated by protein kinase C (PKC)...
January 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27951487/synthesis-and-antiproliferative-activity-of-monocationic-arylthiophene-derivatives
#15
Mohamed A Ismail, Magdy M Youssef, Reem K Arafa, Shar S Al-Shihry, Wael M El-Sayed
Eleven compounds of substituted 4-(5-arylthiophen-2-yl)benzamidines 4a-k were synthesized from their corresponding mononitriles via treatment with lithium trimethylsilylamide and subsequent de-protection with ethanol/hydrogen chloride. In vitro antiproliferative activities of the new monocationic arylthiophenes were evaluated against 60 human cell lines at NCI, USA. This class of compounds displayed promising submicromolar antiproliferative activities with the most potent compound being 4i (GI50 and TGI of 0...
December 2, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27942149/the-effects-of-the-intake-of-an-isotonic-sports-drink-before-orienteering-competitions-on-skeletal-muscle-damage
#16
Filiz Fatma Colakoglu, Banu Cayci, Metin Yaman, Selma Karacan, Suleyman Gonulateş, Gökhan Ipekoglu, Fatmanur Er
[Purpose] The purpose of this study was to investigate the effects of the intake of an isotonic sports drink (500 ml water, 32 gr carbonhydrate, 120 mg calcium, 248 mg chloride, 230 mg sodium) the level of the skeletal muscle damage of orienteering athletes. [Subjects and Methods] The study was carried out on 21 male elite orienteering athletes. The athletes were divided into two groups by randomized double-blind selection. The experimental group (n=11) was given the isotonic sports drink, while the placebo group (n=10) was given 500 ml pure water...
November 2016: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/27941167/protective-effects-of-dietary-selenium-and-vitamin-c-in-barium-induced-cardiotoxicity
#17
Awatef Elwej, Imen Ghorbel, Mariem Chaabane, Nejla Soudani, Rim Marrekchi, Kamel Jamoussi, Hela Mnif, Tahia Boudawara, Najiba Zeghal, Mediha Sefi
Several metals including barium (Ba) known as environmental pollutants provoke deleterious effects on human health. The present work pertains to the potential ability of selenium (Se) and/or vitamin C, used as nutritional supplements, to alleviate the toxic effects induced by barium chloride (BaCl2) in the heart of adult rats. Animals were randomly divided into seven groups of six each: group 1, serving as negative controls, received distilled water; group 2 received in their drinking water BaCl2 (67 ppm); group 3 received both Ba and Se (sodium selenite 0...
December 9, 2016: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/27939722/the-effects-of-linalool-on-the-excitability-of-central-neurons-of-snail-caucasotachea-atrolabiata
#18
Jafar Vatanparast, Sara Bazleh, Mahyar Janahmadi
Linalool is a major constituent of the essential oil of several plant species and possesses several biological activities. In this work, we studied the effects of linalool on excitability of central neurons of land snail Caucasotachea atrolabiata and tried to elucidate the underlying mechanisms. The lower concentration of linalool (0.1mM) showed suppressive action on spontaneous activity and pentylenetetrazole-induced epileptiform activity. These effects were associated with elevation of the action potential threshold and reduction of action potential rising phase, supporting the inhibitory action of linalool on Na(+) channels...
February 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/27930548/primary-central-nervous-system-alk-positive-anaplastic-large-cell-lymphoma-in-an-adult-a-rare-case-report
#19
Xiaoqin Dong, Jun Li, Na Huo, Yan Wang, Zhao Wu, Xiaohong Lin, Hong Zhao
RATIONALE: Anaplastic large cell lymphoma (ALCL) is an aggressive non-Hodgkin lymphoma. It mostly invades lymph nodes with extranodal involvement observed in the soft tissue, bone, and skin. PATIENT CONCERNS: We report a 34-year-old Chinese male patient who presented with headache, diplopia, and vomit. Cerebrospinal fluid (CSF) analysis via lumbar puncture showed elevated CSF pressure, elevated CSF protein concentrations, decreased CSF glucose and chloride concentration significantly, and pleocytosis of 68 to 350 × 10/L, in which lymphocytes and monocytes were predominant...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27922694/cpkc%C3%AE-membrane-translocation-is-involved-in-herkinorin%C3%A2-induced-neuroprotection-against-cerebral-ischemia-reperfusion-injury-in-mice
#20
Xiaochen Gui, Xu Cui, Haiping Wei, Guang Feng, Xuezheng Zhang, Yongjin He, Junfa Li, Tianzuo Li
Herkinorin is an opiate analgesic with limited adverse effects, functioning as a primary selective atypical opioid µ agonist. The present study aimed to identify whether herkinorin has a positive effect on ischemic/reperfusion (I/R) injury. Adult male C57BL/6 mice were randomly divided into five groups: i) Naïve, ii) sham, iii) I/R, iv) I/R with dimethyl sulfoxide (I/R+D) and v) I/R with herkinorin (I/R+H). The I/R injury model was induced by occluding the middle cerebral artery for 1 h followed by 24 h or 7 days of reperfusion...
January 2017: Molecular Medicine Reports
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