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https://www.readbyqxmd.com/read/29156109/a-microfabricated-96-well-wound-healing-assay
#1
Shaoliang Luan, Rui Hao, Yuanchen Wei, Deyong Chen, Beiyuan Fan, Fengliang Dong, Wei Guo, Junbo Wang, Jian Chen
This article presents a microfabricated 96-well wound-healing assay enabling high-throughput measurement of cellular migration capabilities. Within each well, the middle area is the wound region, made of microfabricated gold surface with self-assembled PEG repellent for cell seeding. After the formation of a cellular confluent monolayer around the wound region, collagen solution was applied to form three-dimensional matrix to cover the PEG surface, initiating the wound-healing process. By interpreting the numbers of migrated cells into the wound regions as a function of specific stimuli with different concentrations, EC50 (half-maximal effective concentration) was obtained...
November 20, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29096610/therapy-response-testing-of-breast-cancer-in-a-3d-high-throughput-perfused-microfluidic-platform
#2
Henriette L Lanz, Anthony Saleh, Bart Kramer, Junmei Cairns, Chee Ping Ng, Jia Yu, Sebastiaan J Trietsch, Thomas Hankemeier, Jos Joore, Paul Vulto, Richard Weinshilboum, Liewei Wang
BACKGROUND: Breast cancer is the most common invasive cancer among women. Currently, there are only a few models used for therapy selection, and they are often poor predictors of therapeutic response or take months to set up and assay. In this report, we introduce a microfluidic OrganoPlate® platform for extracellular matrix (ECM) embedded tumor culture under perfusion as an initial study designed to investigate the feasibility of adapting this technology for therapy selection. METHODS: The triple negative breast cancer cell lines MDA-MB-453, MDA-MB-231 and HCC1937 were selected based on their different BRCA1 and P53 status, and were seeded in the platform...
November 2, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29077384/modeling-glyco-collagen-conjugates-using-host-guest-strategy-to-alter-phenotypic-cell-migration-and-in-vivo-wound-healing
#3
Sivakoti Sangabathuni, Raghavendra Vasudeva Murthy, Madhuri Gade, Harikrishna Bavireddi, Suraj Toraskar, Mahesh V Sonar, Krishna N Ganesh, Raghavendra Kikkeri
The constructs and study of combinatorial libraries of structurally defined homologs extracellular matrix (ECM) glycopeptides can significantly accelerate the identification of cell surface markers involved in a variety of physiological and pathological processes. Herein, we present a simple and reliable host-guest approach to design high-throughput glyco-collagen library to modulate the primary and secondary cell lines migration process. 4-Amidoadamantyl-substituted collagen peptides and β-cyclodextrin (β-CD) appended with mono or disaccharides were used to construct self-assembled glyco-collagen conjugates (GCC) which were found to be thermally stable, with a triple helix structures and nanoneedles-like morphologies that altered cell migration processes...
October 27, 2017: ACS Nano
https://www.readbyqxmd.com/read/29069654/expression-profiles-of-long-noncoding-rna-in-uva-induced-human-skin-fibroblasts
#4
Yue Zheng, Qingfang Xu, Yating Peng, Zijian Gong, Haiyan Chen, Wei Lai, Howard I Maibach
BACKGROUND: Long noncoding RNA (lncRNA) are differentially expressed across stages of differentiation and development, but the role of lncRNA in human skin photoaging mechanisms remains poorly understood. OBJECTIVE: This study aimed to determine lncRNA expression changes in human dermal fibroblasts (HDF) induced by repeated UVA irradiation and to explore correlations between lncRNA and skin photoaging prognosis. METHODS: In the UVA-HDF group, HDF were subjected to repeated UVA irradiation (10 J/cm2 UVA twice daily for 7 days); in the control group, HDF received no irradiation...
October 26, 2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/29056521/study-on-the-role-of-hsa-mir-31-5p-in-hypertrophic-scar-formation-and-the-mechanism
#5
X Wang, Y Zhang, B H Jiang, Q Zhang, R P Zhou, L Zhang, Wang Chen
Hypertrophic scar (HS) formation is associated with the fibrosis of fibrocytes caused by excessive extracellular matrix (ECM) synthesis and deposition, the initial event of HS formation. Our high throughput screen of miRNA expression profiles identified hsa-miR31-5p, whose transcription level was most differentially in normal skin fibroblasts (NS) and HS among other miRNAs. The level of hsa-miR31-5p in HS was significantly higher than in NS. In-vitro functional experiments showed hsa-miR31-5p knockdown remarkably suppressed the proliferation of hypertrophic scar fibroblasts (HSFBs) under hypoxia, promoted cell invasion, and inhibited the expression of Collagen I and III and Fibronectin (FN), suggesting that hsa-miR31-5p knockdown effectively reduces HS formation caused by excessive ECM synthesis and deposition in HSFBs under hypoxia...
October 19, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28962209/effects-of-insulin-like-growth-factor-1-and-basic-fibroblast-growth-factor-on-the-morphology-and-proliferation-of-chondrocytes-embedded-in-matrigel-in-a-microfluidic-platform
#6
Yuancheng Li, Qinbo Fan, Yong Jiang, Fuliang Gong, Honggang Xia
An integrated microfluidic device was utilized in the present study to investigate the morphology and proliferation of rabbit articular chondrocytes embedded in Matrigel in the presence of insulin-like growth factor 1 (IGF-1) and/or basic fibroblast growth factor (bFGF). The microfluidic device was composed of two parallel channels and a central perfusion-based three-dimensional cell culture module. The rabbit chondrocytes were cultured for 2 weeks at series of concentration gradients of IGF-1 and/or bFGF, which were generated through a diffusion process...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28829951/collagenase-treatment-enhances-proteomic-coverage-of-low-abundance-proteins-in-decellularized-matrix-bioscaffolds
#7
Miljan Kuljanin, Cody F C Brown, Matthew J Raleigh, Gilles A Lajoie, Lauren E Flynn
There is great interest in the application of advanced proteomic techniques to characterize decellularized tissues in order to develop a deeper understanding of the effects of the complex extracellular matrix (ECM) composition on the cellular response to these pro-regenerative bioscaffolds. However, the identification of proteins in ECM-derived bioscaffolds is hindered by the high abundance of collagen in the samples, which can interfere with the detection of lower-abundance constituents that may be important regulators of cell function...
August 13, 2017: Biomaterials
https://www.readbyqxmd.com/read/28816097/-meltblown-polymer-fabrics-as-candidate-scaffolds-for-rotator-cuff-tendon-tissue-engineering
#8
Thomas L Jenkins, Sean Meehan, Behnam Pourdeyhimi, Dianne Little
Various biomaterial technologies are promising for tissue engineering, including electrospinning, but commercial scale-up of throughput is difficult. The goal of the study was to evaluate meltblown fabrics as candidate scaffolds for rotator cuff tendon tissue engineering. Meltblown poly(lactic acid) fabrics were produced with several polymer crystallinities and airflow velocities [500(low), 900(medium) or 1400(high) m(3)air/h/m fabric]. Fiber diameter, alignment, and baseline bidirectional tensile mechanical properties were evaluated...
September 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28726916/a-novel-3-d-bio-microfluidic-system-mimicking-in-vivo-heterogeneous-tumour-microstructures-reveals-complex-tumour-stroma-interactions
#9
Qihui Fan, Ruchuan Liu, Yang Jiao, Chunxiu Tian, James D Farrell, Wenwen Diao, Xiaochen Wang, Fengrong Zhang, Wei Yuan, Haibo Han, Jinfeng Chen, Yue Yang, Xixiang Zhang, Fangfu Ye, Ming Li, Zhongcan Ouyang, Liyu Liu
A 3-D microfluidic system consisting of microchamber arrays embedded in a collagen hydrogel with tuneable biochemical gradients that mimics the tumour microenvironment of mammary glands was constructed for the investigation on the interactions between invasive breast cancer cells and stromal cells. The hollow microchambers in collagen provide a very similar 3-D environment to that in vivo that regulates collective cellular dynamics and behaviour, while the microfluidic channels surrounding the collagen microchamber arrays allow one to impose complex concentration gradients of specific biological molecules or drugs...
August 8, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28692652/developmental-transcriptomic-analyses-for-mechanistic-insights-into-critical-pathways-involved-in-embryogenesis-of-pelagic-mahi-mahi-coryphaena-hippurus
#10
Elvis Genbo Xu, Edward M Mager, Martin Grosell, John D Stieglitz, E Starr Hazard, Gary Hardiman, Daniel Schlenk
Mahi-mahi (Coryphaena hippurus) is a commercially and ecologically important species of fish occurring in tropical and temperate waters worldwide. Understanding early life events is crucial for predicting effects of environmental stress, which is largely restricted by a lack of genetic resources regarding expression of early developmental genes and regulation of pathways. The need for anchoring developmental stages to transcriptional activities is highlighted by increasing evidence on the impacts of recurrent worldwide oil spills in this sensitive species during early development...
2017: PloS One
https://www.readbyqxmd.com/read/28595551/nmr-based-drug-development-and-improvement-against-malignant-melanoma-implications-for-the-mia-protein-family
#11
REVIEW
Oliver Arnolds, Xueyin Zhong, King Tuo Yip, Miriam Schöpel, Bastian Kohl, Stefanie Pütz, Raid Abdel-Jalil, Raphael Stoll
The Melanoma Inhibitory Activity (MIA) protein is strongly expressed and secreted by malignant melanoma cells and was shown to promote melanoma development and invasion. The MIA protein was the first extracellular protein shown to adopt an Src homology 3 (SH3) domain-like fold in solution that can bind to fibronectin type III domains. Together with MIA, the homologous proteins OTOR (or FDP), MIA-2, and TANGO (or MIA-3) constitute a protein family of non-cytosolic and - except for fulllength TANGO and TANGO1-like (TALI) - extracellular SH3-domain containing proteins...
2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28559982/long-noncoding-rnas-and-novel-inflammatory-genes-determined-by-rna-sequencing-in-human-lymphocytes-are-up-regulated-in-permanent-atrial-fibrillation
#12
Xue-Jing Yu, Li-Hui Zou, Jun-Hua Jin, Fei Xiao, Li Li, Nan Liu, Jie-Fu Yang, Tong Zou
Atrial fibrillation (AF) is a common arrhythmia in clinical practice. Currently, approximately 33.5 million individuals are affected by AF globally. AF involves multiple complicated mechanisms which have not been fully investigated yet. RNA sequencing (RNAseq) is an outstanding method for investigation of diseases due to its high-throughput information. Here, RNAseq was applied to determine mRNA and long noncoding RNA (lncRNA) expression profiles in human lymphocytes from 6 permanent atrial fibrillation (pmAF) patients and 6 healthy controls...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28455445/the-soluble-protease-adamdec1-released-from-activated-platelets-hydrolyzes-platelet-membrane-pro-epidermal-growth-factor-egf-to-active-high-molecular-weight-egf
#13
Rui Chen, Ge Jin, Thomas M McIntyre
Platelets are the sole source of EGF in circulation, yet how EGF is stored or released from stimulated cells is undefined. In fact, we found platelets did not store EGF, synthesized as a single 6-kDa domain in pro-EGF, but rather expressed intact pro-EGF precursor on granular and plasma membranes. Activated platelets released high-molecular-weight (HMW)-EGF, produced by a single cleavage between the EGF and the transmembrane domains of pro-EGF. We synthesized a fluorogenic peptide encompassing residues surrounding the putative sessile arginyl residue and found stimulated platelets released soluble activity that cleaved this pro-EGF1020-1027 peptide...
June 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28448052/an-in-vitro-organ-culture-model-of-the-murine-intervertebral-disc
#14
Jennifer W Liu, Kevin H Lin, Christian Weber, Sameer Bhalla, Sean Kelso, Kaixi Wang, Simon Y Tang
Intervertebral disc (IVD) degeneration is a significant contributor to low back pain. The IVD is a fibrocartilaginous joint that serves to transmit and dampen loads in the spine. The IVD consists of a proteoglycan-rich nucleus pulposus (NP) and a collagen-rich annulus fibrosis (AF) sandwiched by cartilaginous end-plates. Together with the adjacent vertebrae, the vertebrae-IVD structure forms a functional spine unit (FSU). These microstructures contain unique cell types as well as unique extracellular matrices...
April 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28444343/lysolecithin-as-feed-additive-enhances-collagen-expression-and-villus-length-in-the-jejunum-of-broiler-chickens
#15
D L Brautigan, R Li, E Kubicka, S D Turner, J S Garcia, M L Weintraut, E A Wong
Adding lysolecithin to feed has reportedly improved the performance of broiler chickens. Lysolecithin is generated by phospholipase catalyzed hydrolysis of lecithin. The enzymatic reaction converts various phospholipids into the corresponding lysophospholipids, with lysophosphatidylcholine (LPC) one of the primary products. Here we compared supplementation with a commercial lysolecithin (Lysoforte®) with comparable levels of highly purified LPC for effects on broilers. Despite no differences in weight gain during the starter period, we discovered a significant increase in average villus length with lysolecithin and an increase in villus width with purified LPC...
August 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28406494/laser-capture-microdissection-of-pancreatic-acinar-cells-to-identify-proteomic-alterations-in-a-murine-model-of-caerulein-induced-pancreatitis
#16
John P Shapiro, Hannah M Komar, Baris Hancioglu, Lianbo Yu, Ming Jin, Yuko Ogata, Phil A Hart, Zobeida Cruz-Monserrate, Gregory B Lesinski, Darwin L Conwell
OBJECTIVES: Chronic pancreatitis (CP) is characterized by inflammation and fibrosis of the pancreas, leading to pain, parenchymal damage, and loss of exocrine and endocrine function. There are currently no curative therapies; diagnosis remains difficult and aspects of pathogenesis remain unclear. Thus, there is a need to identify novel biomarkers to improve diagnosis and understand pathophysiology. We hypothesize that pancreatic acinar regions contain proteomic signatures relevant to disease processes, including secreted proteins that could be detected in biofluids...
April 13, 2017: Clinical and Translational Gastroenterology
https://www.readbyqxmd.com/read/28393762/engineering-a-functional-three-dimensional-human-cardiac-tissue-model-for-drug-toxicity-screening
#17
Hong Fang Lu, Meng Fatt Leong, Tze Chiun Lim, Ying Ping Chua, Jia Kai Lim, Chan Du, Andrew C A Wan
Cardiotoxicity is one of the major reasons for clinical drug attrition. In vitro tissue models that can provide efficient and accurate drug toxicity screening are highly desired for preclinical drug development and personalized therapy. Here, we report the fabrication and characterization of a human cardiac tissue model for high throughput drug toxicity studies. Cardiac tissues were fabricated via cellular self-assembly of human transgene-free induced pluripotent stem cells-derived cardiomyocytes in pre-fabricated polydimethylsiloxane molds...
May 11, 2017: Biofabrication
https://www.readbyqxmd.com/read/28355300/evaluation-of-synthetic-vascular-grafts-in-a-mouse-carotid-grafting-model
#18
Alex H P Chan, Richard P Tan, Praveesuda L Michael, Bob S L Lee, Laura Z Vanags, Martin K C Ng, Christina A Bursill, Steven G Wise
Current animal models for the evaluation of synthetic grafts are lacking many of the molecular tools and transgenic studies available to other branches of biology. A mouse model of vascular grafting would allow for the study of molecular mechanisms of graft failure, including in the context of clinically relevant disease states. In this study, we comprehensively characterise a sutureless grafting model which facilitates the evaluation of synthetic grafts in the mouse carotid artery. Using conduits electrospun from polycaprolactone (PCL) we show the gradual development of a significant neointima within 28 days, found to be greatest at the anastomoses...
2017: PloS One
https://www.readbyqxmd.com/read/28337850/high-throughput-screening-of-vascular-endothelium-destructive-or-protective-microenvironments-cooperative-actions-of-extracellular-matrix-composition-stiffness-and-structure
#19
Yonghui Ding, Michael Floren, Wei Tan
Pathological modification of the subendothelial extracellular matrix (ECM) has closely been associated with endothelial activation and subsequent cardiovascular disease progression. To understand regulatory mechanisms of these matrix modifications, the majority of previous efforts have focused on the modulation of either chemical composition or matrix stiffness on 2D smooth surfaces without simultaneously probing their cooperative effects on endothelium function on in vivo like 3D fibrous matrices. To this end, a high-throughput, combinatorial microarray platform on 2D and 3D hydrogel settings to resemble the compositions, stiffness, and structure of healthy and diseased subendothelial ECM has been established, and further their respective and combined effects on endothelial attachment, proliferation, inflammation, and junctional integrity have been investigated...
June 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28322869/scaffold-free-high-throughput-generation-of-quiescent-valvular-microtissues
#20
Annelies Roosens, Inès Puype, Ria Cornelissen
AIMS: The major challenge of working with valvular interstitial cells in vitro is the preservation or recovery of their native quiescent state. In this study, a biomimetic approach is used which aims to engineer small volume, high quality valve microtissues, having a potential in regenerative medicine and as a relevant 3D in vitro model to provide insights into valve (patho)biology. METHODS AND RESULTS: To form micro-aggregates, porcine valvular interstitial cells were seeded in agarose micro-wells and cultured in medium supplemented with 250μM Ascorbic Acid 2-phosphate for 22days...
March 18, 2017: Journal of Molecular and Cellular Cardiology
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