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https://www.readbyqxmd.com/read/28320535/optimization-of-selective-pressurized-liquid-extraction-of-organic-pollutants-in-placenta-to-evaluate-prenatal-exposure
#1
REVIEW
Tania Fernández-Cruz, Elena Martínez-Carballo, Jesús Simal-Gándara
The early exposure to organic pollutants (OPs) related to dietary habit or environmental exposure is a concern of growing interest in environmental health. When OPs enter the body, they can accumulate in fatty tissue and even travel through the bloodstream being able to cross the placenta and reach the fetus through the substance exchange between the mother and the child. Epidemiologic and clinical data affirm that these chemicals increase the risk of adverse effects during childhood. This article reviews and addresses one of the most relevant analytical methods for determining OPs in placenta...
March 8, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28293436/placental-vitamin-d-binding-protein-expression-in-human-idiopathic-fetal-growth-restriction
#2
Alice F Wookey, Tejasvy Chollangi, Hannah E J Yong, Bill Kalionis, Shaun P Brennecke, Padma Murthi, Harry M Georgiou
Vitamin D-binding protein is a multifunctional serum protein with multiple actions related to normal health. Vitamin D-binding protein transports vitamin D and influences the metabolism of this key hormone but it also has additional immunomodulatory and actin-clearing properties. We investigated whether vitamin D-binding protein expression is altered in fetal growth restriction-associated placental dysfunction. Protein was extracted from 35 placentae derived from 17 healthy control subjects and 18 gestation-matched subjects with fetal growth restriction (FGR)...
2017: Journal of Pregnancy
https://www.readbyqxmd.com/read/28282771/the-follicle-stimulating-hormone-receptor-asn680ser-polymorphism-is-associated-with-preterm-birth-in-hispanic-women
#3
Pablo Dominguez-Lopez, Laura Diaz-Cueto, Miguel Arechavaleta-Velasco, Felipe Caldiño-Soto, Alfredo Ulloa-Aguirre, Fabian Arechavaleta-Velasco
OBJECTIVE: Recently, a study based on the analysis of accelerated evolution of related genes at birth identified the follicle-stimulating hormone receptor (FSHR) as a possible candidate for the development of preterm delivery. Additionally, FSHR expression has been described in extragonadal tissue including the placenta. Therefore, the aim of the present study was to determine the association between the N680S polymorphism of the follicle-stimulating hormone receptor and preterm birth in a population of Hispanic women...
February 28, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28273703/-the-composition-of-the-gastrointestinal-bacterial-flora-of-mouse-embryos-and-the-placenta-tissue-bacterial-flora
#4
D Lei, Y Lin, X Jiang, L Lan, W Zhang, B X Wang
Objective: To explore the composition of the gastrointestinal bacterial flora of mouse embryos and the placenta tissue bacterial flora. Method: Twenty-four specimens were collected from pregnant Kunming mouse including 8 mice of early embryonic (12-13 days) gastrointestinal tissues, 8 cases of late embryonic (19-20 days)gastrointestinal tissues, 8 of late pregnancy placental tissues.The 24 samples were extracted by DNeasy Blood & Tissue kit for high-throughput DNA sequencing. Result: The level of Proteobacteria, Bacteroidetes, Actino-bacteria and Firmicutes were predominantin all specimens...
March 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28252405/placenta-maps-in-utero-placental-health-assessment-of-the-human-fetus
#5
Haichao Miao, Gabriel Mistelbauer, Alexey Karimov, Amir Alansary, Alice Davidson, David Lloyd, Mellisa Damodaram, Lisa Story, Jana Hutter, Joseph Hajnal, Mary Rutherford, Bernhard Preim, Bernhard Kainz, M Eduard Groller
The human placenta is essential for the supply of the fetus. To monitor the fetal development, imaging data is acquired using ultrasound (US). Although it is currently the gold-standard in fetal imaging, it might not capture certain abnormalities of the placenta. Magnetic resonance imaging (MRI) is a safe alternative for the in utero examination while acquiring the fetus data in higher detail. Nevertheless, there is currently no established procedure for assessing the condition of the placenta and consequently the fetal health...
February 24, 2017: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/28202794/evaluation-of-the-toxoplasma-elite-mgb%C3%A2-real-time-pcr-assay-for-the-diagnosis-of-toxoplasmosis
#6
Florence Robert-Gangneux, Marie-Pierre Brenier-Pinchart, Hélène Yera, Sorya Belaz, Emmanuelle Varlet-Marie, Patrick Bastien
Molecular diagnosis of congenital toxoplasmosis or disseminated toxoplasmosis is mainly based on PCR. The repeated DNA element rep529 has become the main DNA target used in most, whether laboratory-developed or commercial, PCR methods. In this multicenter study, we evaluated the Toxoplasma ELITe MGB® (Elitech®) commercial kit, by comparison with three reference quantitative PCR assays (RA) used in routine in three proficient laboratories of the French National Reference Center for Toxoplasmosis network, using Toxoplasma calibrated suspensions diluted to obtain a range of concentrations from 0...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28188612/hepatitis-b-immunoglobulin-during-pregnancy-for-prevention-of-mother-to-child-transmission-of-hepatitis-b-virus
#7
REVIEW
Ahizechukwu C Eke, George U Eleje, Uzoamaka A Eke, Yun Xia, Jiao Liu
BACKGROUND: Hepatitis is a viral infection of the liver. It is mainly transmitted between people through contact with infected blood, frequently from mother to baby in-utero. Hepatitis B poses significant risk to the fetus and up to 85% of infants infected by their mothers at birth develop chronic hepatitis B virus (HBV) infection. Hepatitis B immunoglobulin (HBIG) is a purified solution of human immunoglobulin that could be administered to the mother, newborn, or both. HBIG offers protection against HBV infection when administered to pregnant women who test positive for hepatitis B envelope antigen (HBeAg) or hepatitis B surface antigen (HBsAg), or both...
February 11, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28181672/endometriosis-increases-the-risk-of-obstetrical-and-neonatal-complications
#8
Janne Foss Berlac, Dorthe Hartwell, Charlotte Wessel Skovlund, Jens Langhoff-Roos, Øjvind Lidegaard
INTRODUCTION: The objective was to assess obstetrical complications and neonatal outcomes in women with endometriosis as compared to women without endometriosis. MATERIAL AND METHODS: National cohort including all delivering women and their newborns in Denmark 1997-2014. Data were extracted from the Danish Health Register and the Medical Birth Register. Logistic regression analysis provided odds ratios (OR) with 95% confidence intervals (CI). Sub-analyses were made for primiparous women with a singleton pregnancy and for women with endometriosis who underwent gynecological surgery before pregnancy...
February 9, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28153653/prenatal-diagnosis-and-management-of-vasa-previa-in-twin-pregnancies-a%C3%A2-case-series-and%C3%A2-systematic-review
#9
REVIEW
Eric Jauniaux, Yaakov Melcer, Ron Maymon
BACKGROUND: Twin pregnancies are at higher risks of velamentous cord insertion and vasa previa. In vitro fertilization is an additional risk factor of abnormal cord insertion and thus the incidence of vasa previa is likely to increase over the next decades. OBJECTIVE: We sought to evaluate the role of ultrasound imaging in optimizing the management of twins diagnosed with vasa previa antenatally. STUDY DESIGN: We searched our database for twin pregnancies diagnosed with vasa previa and managed antenatally using measurements of cervical length and performed a systematic review of articles that correlated prenatal diagnosis of vasa previa in twins and pregnancy outcome...
January 30, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28138995/time-course-analysis-of-rna-quality-in-placenta-preserved-by-rnalater-or-flash-freezing
#10
Nicole M Martin, Katherine M Cooke, Caitlin C Radford, Lauren E Perley, Michelle Silasi, Clare A Flannery
PROBLEM: Preservation of biospecimen quality is critical to accurately and reliably assessing genes and proteins. We evaluated the effect of preparation method and storage duration on RNA quality in placenta and decidua. METHOD OF STUDY: Aliquots of nine placentas and decidua were placed in RNAlater(®) (RL) or flash frozen (FF) within 30 minutes of delivery. RNA was extracted immediately (baseline) and from matched samples stored at -80°C for 1 and 8-10 months...
January 31, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28130302/distinct-growth-and-secretome-strategies-by-two-taxonomically-divergent-brown-rot-fungi
#11
Gerald N Presley, Jonathan S Schilling
Brown rot fungi are wood-degrading fungi that employ both oxidative and hydrolytic mechanisms to degrade wood. Hydroxyl radicals that facilitate the oxidative component are powerful non-selective oxidants and are incompatible with hydrolytic enzymes unless they are spatially segregated in wood. Differential gene expression has been implicated in Postia placenta to segregate these reactions, but it is unclear if this two-step mechanism varies in other brown rot fungi with different traits and life history strategies, and that occupy different niches in nature...
January 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28129936/assessment-of-parabens-and-ultraviolet-filters-in-human-placenta-tissue-by-ultrasound-assisted-extraction-and-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#12
F Vela-Soria, M E Gallardo-Torres, O Ballesteros, C Díaz, J Pérez, A Navalón, M F Fernández, N Olea
Increasing concerns have been raised over recent decades about human exposure to Endocrine Disrupting Chemicals (EDCs), especially about their possible effects on embryo, foetus, newborn, and child. Parabens (PBs) and ultraviolet filters (UV-filters) are prevalent EDCs widely used as additives in cosmetics and personal care products (PCPs). The objective of this study was to determine the presence of four PBs and ten UV-filters in placental tissue samples using a novel analytical method based on ultrasound-assisted extraction (UAE) and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)...
March 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28124800/trans-fatty-acid-accumulation-in-the-human-placenta
#13
Yoshiro Wada, Shumi Yoshida-Yamamoto, Yoshinao Wada, Masahiro Nakayama, Nobuaki Mitsuda, Hiroyuki Kitajima
Trans fatty acid may impair fetal growth and infant neurodevelopment, but the quantity in a placenta and human tissues remains unknown. To address the issue, a simple and reliable method of quantification is needed. We established a method of quantifying trans-octadecenoic acids (trans-6,8,9,11 18:1 fatty acids, TOAs), a major component of trans fatty acid, in human tissue samples, and then determined the TOAs level in the placenta. Oleic acid (OA) (C18:1(9c)) was measured by isotope dilution gas chromatography-mass spectrometry, and the TOAs level was subsequently calculated based on the ratio of the peak areas for TOAs and OA (TOAs/OA) in the mass chromatogram...
March 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28092076/determination-of-trace-elements-in-cow-placenta-by-tungsten-coil-atomic-emission-spectrometry
#14
Daniel A Gonçalves, Ana C Soncin, George L Donati, Mirian C Dos Santos
Tungsten coil atomic emission spectrometry (WCAES) is used to determine trace levels of Mn (403.1 nm) and Cr (425.5 nm) in cow placenta. All samples were collected in Ilha Solteira, SP, Brazil. The instrumental setup is based on a tungsten filament extracted from 150 W, 15 V microscope light bulbs, a solid state power supply, fused silica lens, crossed Czerny-Turner spectrograph, and a thermoelectrically cooled charge-coupled device detector. The limits of detection (LOD) and quantification (LOQ) for Cr are 2 and 8 μg L(-1), and 20 and 60 μg L(-1) for Mn, respectively...
January 16, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/27965171/human-placenta-hydrogel-reduces-scarring-in-a-rat-model-of-cardiac-ischemia-and-enhances-cardiomyocyte-and-stem-cell-cultures
#15
Michael P Francis, Erick Breathwaite, Anna A Bulysheva, Frency Varghese, Rudy U Rodriguez, Sucharita Dutta, Iurii Semenov, Rebecca Ogle, Alexander Huber, Alexandra-Madelaine Tichy, Silvia Chen, Christian Zemlin
INTRODUCTION: Xenogeneic extracellular matrix (ECM) hydrogels have shown promise in remediating cardiac ischemia damage in animal models, yet analogous human ECM hydrogels have not been well development. An original human placenta-derived hydrogel (hpECM) preparation was thus generated for assessment in cardiomyocyte cell culture and therapeutic cardiac injection applications. METHODS AND RESULTS: Hybrid orbitrap-quadrupole mass spectrometry and ELISAs showed hpECM to be rich in collagens, basement membrane proteins, and regenerative growth factors (e...
December 10, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27933483/covalent-immobilization-of-human-placental-17%C3%AE-hydroxysteroid-dehydrogenase-type-1-onto-glutaraldehyde-activated-silica-coupled-with-lc-tof-ms-for-anti-cancer-drug-screening-applications
#16
Yin Bai, Wen-Di Zhou, Xian-Min Mu, Qian Zhang, Chen Yu, Bin Di, Meng-Xiang Su
Human 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1), a potential target in breast cancer prevention and therapy, was extracted from human placenta and immobilized on nonporous silica (∼5 μm) with a covalent method for the first time. The optimum initial enzyme concentration and immobilization time during the immobilization process were 0.42 mg mL(-1) and 12 h, repectively. The binding was confirmed by scanning electron microscope (SEM) and infrared spectroscopy (FT-IR). It could improve the pH, thermal and storage stability compared to free enzyme...
December 8, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27904431/term-live-secondary-abdominal-pregnancy-a-case-report
#17
Deepika Pannu, Rekha Bharti, H P Anand, Manjula Sharma
Term, live abdominal pregnancy secondary to rupture of a uterine rudimentary horn is a rare condition. Pregnancies conceived in the rudimentary horn of the uterus usually rupture during early gestation and present as a catastrophic event. However, rarely, after rupture of the uterine horn the foetus may continue to grow in the abdominal cavity and reach term gestation. A primigravida with a term pregnancy was referred to our centre for caesarean section with ultrasonography findings of transverse lie and placenta previa...
September 2016: Malaysian Journal of Medical Sciences: MJMS
https://www.readbyqxmd.com/read/27887588/longitudinal-reference-ranges-for-maternal-plasma-laeverin-and-its-role-as-a-potential-biomarker-of-preeclampsia
#18
Mona Nystad, Vasilis Sitras, Kari Flo, Christian Widnes, Åse Vårtun, Tom Wilsgaard, Ganesh Acharya
BACKGROUND: Laeverin is a placenta-specific membrane-bound aminopeptidase. In this study we wanted to: 1) serially measure plasma levels of laeverin in healthy women during the second half of pregnancy and postpartum, 2) determine whether laeverin is differently expressed at 22-24 weeks in women who later develop preeclampsia compared to controls, 3) compare laeverin protein expression in placenta and umbilical vein serum in healthy and preeclamptic pregnancies at birth. METHODS: Plasma was obtained serially, approximately every 4-weeks, from 53 healthy women with uncomplicated pregnancies during 22(+0) to 39(+6) weeks of gestation, and at 22-24 weeks from 15 women who later developed preeclampsia...
November 25, 2016: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/27825028/peripartum-hysterectomy-incidence-risk-factors-and-clinical-characteristics-in-ireland
#19
Sarah M Campbell, Paul Corcoran, Edel Manning, Richard A Greene
BACKGROUND: The incidence of peripartum hysterectomy (PH) shows fifty-fold variation worldwide (0.2-10.5/1000 deliveries) and risk factors include advancing maternal age and parity, previous caesarean section (CS) and abnormal placentation. OBJECTIVES: In this first national study of PH in Ireland, our objectives were threefold: to describe the national trend in PH incidence over 15 years since 1999; to assess risk of PH associated with morbidly adherent placenta (MAP), placenta praevia and postpartum haemorrhage (PPH) during 2005-2013; and to describe the causes, interventions and outcomes of PH cases during 2011-2013...
December 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27817049/overexpression-of-endogenous-anti-oxidants-with-selenium-supplementation-protects-trophoblast-cells-from-reactive-oxygen-species-induced-apoptosis-in-a-bcl-2-dependent-manner
#20
Alisha Khera, Jessica J Vanderlelie, Olivia Holland, Anthony V Perkins
The human placenta provides life support for the developing foetus, and a healthy placenta is a prerequisite to a healthy start to life. Placental tissue is subject to oxidative stress which can lead to pathological conditions of pregnancy such as preeclampsia, preterm labour and intrauterine growth restriction. Up-regulation of endogenous anti-oxidants may alleviate placental oxidative stress and provide a therapy for these complications of pregnancy. In this study, selenium supplementation, as inorganic sodium selenite (NaSel) or organic selenomethionine (SeMet), was used to increase the protein production and cellular activity of the important redox active proteins glutathione peroxidase (GPx) and thioredoxin reductase (Thx-Red)...
November 5, 2016: Biological Trace Element Research
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