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Matthew Martin, Laiqing Hua, Benlian Wang, Han Wei, Lakshmi Prabhu, Antja-Voy Hartley, Guanglong Jiang, Yunlong Liu, Tao Lu
The Y box protein 1 (YBX1) is a well-known oncoprotein which has tumor promoting functions. YBX1 is widely considered to be an attractive therapeutic target in cancer. In order to develop novel therapeutics to target YBX1, it is of great importance to understand how YBX1 is finely regulated in cancer. Previously, we have shown that YBX1 could function as a tumor promoter through phosphorylation of its serine 165 (S165) residue, leading to the activation of the NF-[kappa]B signaling pathway (1). In this study, using mass spectrometry analysis, we discovered a quite distinct phosphorylation site, S176, on YBX1...
January 11, 2017: Journal of Biological Chemistry
Lakshmi Prabhu, Rasika Mundade, Benlian Wang, Han Wei, Antja-Voy Hartley, Matthew Martin, Kyle McElyea, Constance J Temm, George Sandusky, Yunlong Liu, Tao Lu
Y-box binding protein 1 [YBX1] is a multifunctional protein known to facilitate many of the hallmarks of cancer. Elevated levels of YBX1 protein are highly correlated with cancer progression, making it an excellent marker in cancer. The connection between YBX1 and the important nuclear factor κB [NF-κB] has never been reported. Here, we show that overexpression of wild type YBX1 [WT-YBX1] activates NF-κB, suggesting that YBX1 is a potential NF-κB activator. Furthermore, using mass spectrometry analysis we identified novel phosphorylation of serine 165 [S165] on YBX1...
October 6, 2015: Oncotarget
Benlian Wang, Han Wei, Lakshmi Prabhu, Wei Zhao, Matthew Martin, Antja-Voy Hartley, Tao Lu
Nuclear factor κB (NF-κB) is a central coordinator in immune and inflammatory responses. Constitutive NF-κB is often found in some types of cancers, contributing to oncogenesis and tumor progression. Therefore, knowing how NF-κB is regulated is important for its therapeutic control. Post-translational modification of the p65 subunit of NF-κB is a well known approach for its regulation. Here, we reported that in response to interleukin 1β, the p65 subunit of NF-κB is phosphorylated on the novel serine 316...
August 14, 2015: Journal of Biological Chemistry
M V Dodson, R E Allen, M Du, W G Bergen, S G Velleman, S P Poulos, M Fernyhough-Culver, M B Wheeler, S K Duckett, M R I Young, B H Voy, Z Jiang, G J Hausman
If one were to compare today's animal growth research to research from a mere 50 yr ago, one would see programs with few similarities. The evolution of this research from whole-animal through cell-based and finally molecular and genomic studies has been enhanced by the identification, isolation, and in vitro evaluation of adipose- and muscle-derived stem cells. This paper will highlight the struggles and the milestones that make this evolving area of research what it is today. The contribution of adipose and muscle stem cell research to development and growth, tissue regeneration, and final carcass composition are reviewed...
February 2015: Journal of Animal Science
Jörg-Detlef Drenckhahn, Jette Strasen, Kirsten Heinecke, Patrick Langner, Kom Voy Yin, Friederike Skole, Maria Hennig, Bastian Spallek, Robert Fischer, Florian Blaschke, Arnd Heuser, Timothy C Cox, Mary Jane Black, Ludwig Thierfelder
AIMS: Foetal growth has been proposed to influence cardiovascular health in adulthood, a process referred to as foetal programming. Indeed, intrauterine growth restriction in animal models alters heart size and cardiomyocyte number in the perinatal period, yet the consequences for the adult or challenged heart are largely unknown. The aim of this study was to elucidate postnatal myocardial growth pattern, left ventricular function, and stress response in the adult heart after neonatal cardiac hypoplasia in mice...
April 1, 2015: Cardiovascular Research
Bo Ji, Jesse L Middleton, Ben Ernest, Arnold M Saxton, Susan J Lamont, Shawn R Campagna, Brynn H Voy
Domestic broiler chickens rapidly accumulate fat and are naturally hyperglycemic and insulin resistant, making them an attractive model for studies of human obesity. We previously demonstrated that short-term (5 h) fasting rapidly upregulates pathways of fatty acid oxidation in broiler chickens and proposed that activation of these pathways may promote leanness. The objective of the current study was to characterize adipose tissue from relatively lean and fatty lines of chickens and determine if heritable leanness in chickens is associated with activation of some of the same pathways induced by fasting...
May 1, 2014: Physiological Genomics
Eugene Kolker, Vural Özdemir, Lennart Martens, William Hancock, Gordon Anderson, Nathaniel Anderson, Sukru Aynacioglu, Ancha Baranova, Shawn R Campagna, Rui Chen, John Choiniere, Stephen P Dearth, Wu-Chun Feng, Lynnette Ferguson, Geoffrey Fox, Dmitrij Frishman, Robert Grossman, Allison Heath, Roger Higdon, Mara H Hutz, Imre Janko, Lihua Jiang, Sanjay Joshi, Alexander Kel, Joseph W Kemnitz, Isaac S Kohane, Natali Kolker, Doron Lancet, Elaine Lee, Weizhong Li, Andrey Lisitsa, Adrian Llerena, Courtney Macnealy-Koch, Jean-Claude Marshall, Paola Masuzzo, Amanda May, George Mias, Matthew Monroe, Elizabeth Montague, Sean Mooney, Alexey Nesvizhskii, Santosh Noronha, Gilbert Omenn, Harsha Rajasimha, Preveen Ramamoorthy, Jerry Sheehan, Larry Smarr, Charles V Smith, Todd Smith, Michael Snyder, Srikanth Rapole, Sanjeeva Srivastava, Larissa Stanberry, Elizabeth Stewart, Stefano Toppo, Peter Uetz, Kenneth Verheggen, Brynn H Voy, Louise Warnich, Steven W Wilhelm, Gregory Yandl
Biological processes are fundamentally driven by complex interactions between biomolecules. Integrated high-throughput omics studies enable multifaceted views of cells, organisms, or their communities. With the advent of new post-genomics technologies, omics studies are becoming increasingly prevalent; yet the full impact of these studies can only be realized through data harmonization, sharing, meta-analysis, and integrated research. These essential steps require consistent generation, capture, and distribution of metadata...
January 2014: Omics: a Journal of Integrative Biology
Jane Morris, Ashley V Simpson, Steven James Voy
INTRODUCTION: Inpatient treatment for eating disorders is a scarce, expensive resource. We aimed to examine length of stay (LOS) in specialist Scottish inpatient units and to identify relationships between LOS, outcome measures and other factors. METHOD: Audit of 206 admissions (89 adolescents and 117 adults) between 2009 and 2011 to all seven inpatient units in Scotland that specialize in the treatment of eating disorders. Data was collected retrospectively from electronic database and patient case files...
January 2015: Clinical Psychology & Psychotherapy
Xinping Huang, Antja-Voy Hartley, Yishi Yin, Jeremy H Herskowitz, James J Lah, Kerry J Ressler
The adeno-associated virus (AAV) is one of the most useful viral vectors for gene delivery for both in vivo and in vitro applications. A variety of methods have been established to produce and characterize recombinant AAV (rAAV) vectors; however most methods are quite cumbersome and obtaining consistently high titer can be problematic. This protocol describes a triple-plasmid co-transfection approach with 25 kDa linear polyethylenimine (PEI) in 293 T cells for the production of AAV serotype 2. Seventy-two hours post-transfection, supernatant and cells were harvested and purified by a discontinuous iodixanol density gradient ultracentrifugation, then dialyzed and concentrated with an Amicon 15 100,000 MWCO concentration unit...
November 2013: Journal of Virological Methods
Sarah J Fletcher, Nishan S Kalupahana, Morvarid Soltani-Bejnood, Jung Han Kim, Arnold M Saxton, David H Wasserman, Bart De Taeye, Brynn H Voy, Annie Quignard-Boulange, Naima Moustaid-Moussa
Numerous animal and clinical investigations have pointed to a potential role of the renin-angiotensin system (RAS) in the development of insulin resistance and diabetes in conditions of expanded fat mass. However, the mechanisms underlying this association remain unclear. We used a transgenic mouse model overexpressing renin in the liver (RenTgMK) to examine the effects of chronic activation of RAS on adiposity and insulin sensitivity. Hepatic overexpression of renin resulted in constitutively elevated plasma angiotensin II (four- to six-fold increase vs...
2012: Frontiers in Endocrinology
Ben Ernest, Jessica R Gooding, Shawn R Campagna, Arnold M Saxton, Brynn H Voy
BACKGROUND: Metabolomics is an emerging high-throughput approach to systems biology, but data analysis tools are lacking compared to other systems level disciplines such as transcriptomics and proteomics. Metabolomic data analysis requires a normalization step to remove systematic effects of confounding variables on metabolite measurements. Current tools may not correctly normalize every metabolite when the relationships between each metabolite quantity and fixed-effect confounding variables are different, or for the effects of random-effect confounding variables...
2012: BMC Research Notes
Laurene Tetard, Ali Passian, Rubye H Farahi, Brynn H Voy, Thomas Thundat
Exploring the interior of a cell is of tremendous importance in order to assess the effects of nanomaterials on biological systems. Outside of a controlled laboratory environment, nanomaterials will most likely not be conveniently labeled or tagged so that their translocation within a biological system cannot be easily identified and quantified. Ideally, the characterization of nanomaterials within a cell requires a nondestructive, label-free, and subsurface approach. Subsurface nanoscale imaging represents a real challenge for instrumentation...
2012: Methods in Molecular Biology
Bo Ji, Ben Ernest, Jessica R Gooding, Suchita Das, Arnold M Saxton, Jean Simon, Joelle Dupont, Sonia Métayer-Coustard, Shawn R Campagna, Brynn H Voy
BACKGROUND: Domestic broiler chickens rapidly accumulate adipose tissue due to intensive genetic selection for rapid growth and are naturally hyperglycemic and insulin resistant, making them an attractive addition to the suite of rodent models used for studies of obesity and type 2 diabetes in humans. Furthermore, chicken adipose tissue is considered as poorly sensitive to insulin and lipolysis is under glucagon control. Excessive fat accumulation is also an economic and environmental concern for the broiler industry due to the loss of feed efficiency and excessive nitrogen wasting, as well as a negative trait for consumers who are increasingly conscious of dietary fat intake...
2012: BMC Genomics
Jeremy J Jay, John D Eblen, Yun Zhang, Mikael Benson, Andy D Perkins, Arnold M Saxton, Brynn H Voy, Elissa J Chesler, Michael A Langston
BACKGROUND: A wealth of clustering algorithms has been applied to gene co-expression experiments. These algorithms cover a broad range of approaches, from conventional techniques such as k-means and hierarchical clustering, to graphical approaches such as k-clique communities, weighted gene co-expression networks (WGCNA) and paraclique. Comparison of these methods to evaluate their relative effectiveness provides guidance to algorithm selection, development and implementation. Most prior work on comparative clustering evaluation has focused on parametric methods...
2012: BMC Bioinformatics
Joëlle Dupont, Sonia Métayer-Coustard, Bo Ji, Christelle Ramé, Christian Gespach, Brynn Voy, Jean Simon
The role of insulin in chicken adipose tissue appears weak or questionable. In a first study, proximal and distal components of the insulin signaling cascade were characterized in abdominal adipose tissue of fasted or fed chickens for the first time. Similar measurements were performed on epididymal adipose tissue from fasted or fed rats for comparison. Tyrosine phosphorylation of IR beta subunit, IRS-1 and Shc and phosphorylation of downstream components (Akt and MAPK ERK1/2) were significantly reduced as expected by fasting in rat, but not in chicken...
March 1, 2012: General and Comparative Endocrinology
M Garcin, J Coquart, J Salleron, N Voy, R Matran
The purpose of this study was to evaluate the validity of the estimated time limit scale (ETL), which deals with a subjective prediction of how long the current exercise intensity can be maintained, for regulating exercise intensity using typical estimation-production procedure. Thirty-six male athletes performed a continuous incremental test and two discontinuous tests with randomized workloads (estimation tests at 65, 75, 85 and 95% of the maximal power output, and production tests: subjects have to use the ETL values which were collected for each power during the estimation test in order to manually product the corresponding workload)...
June 2012: European Journal of Applied Physiology
Nishan S Kalupahana, Florence Massiera, Annie Quignard-Boulange, Gérard Ailhaud, Brynn H Voy, David H Wasserman, Naima Moustaid-Moussa
Although obesity is associated with overactivation of the white adipose tissue (WAT) renin-angiotensin system (RAS), a causal link between the latter and systemic insulin resistance is not established. We tested the hypothesis that overexpression of angiotensinogen (Agt) from WAT causes systemic insulin resistance via modulation of adipose inflammation. Glucose tolerance, systemic insulin sensitivity, and WAT inflammatory markers were analyzed in mice overexpressing Agt in the WAT (aP2-Agt mice). Proteomic studies and in vitro studies using 3T3-L1 adipocytes were performed to build a mechanistic framework...
January 2012: Obesity
Vivek M Philip, Greta Sokoloff, Cheryl L Ackert-Bicknell, Martin Striz, Lisa Branstetter, Melissa A Beckmann, Jason S Spence, Barbara L Jackson, Leslie D Galloway, Paul Barker, Ann M Wymore, Patricia R Hunsicker, David C Durtschi, Ginger S Shaw, Sarah Shinpock, Kenneth F Manly, Darla R Miller, Kevin D Donohue, Cymbeline T Culiat, Gary A Churchill, William R Lariviere, Abraham A Palmer, Bruce F O'Hara, Brynn H Voy, Elissa J Chesler
Genetic reference populations in model organisms are critical resources for systems genetic analysis of disease related phenotypes. The breeding history of these inbred panels may influence detectable allelic and phenotypic diversity. The existing panel of common inbred strains reflects historical selection biases, and existing recombinant inbred panels have low allelic diversity. All such populations may be subject to consequences of inbreeding depression. The Collaborative Cross (CC) is a mouse reference population with high allelic diversity that is being constructed using a randomized breeding design that systematically outcrosses eight founder strains, followed by inbreeding to obtain new recombinant inbred strains...
August 2011: Genome Research
Brynn H Voy
Genetic variation in a population creates an impressive spectrum of phenotypic diversity, particularly when changes in diet or the environment are imposed on the population. Genome-wide association studies have become a powerful tool for linking sequence variants with overlying systems level phenotypes, but they do not provide insight into the mechanisms through which genetic variation drives phenotypic variation. Systems genetics is an emerging discipline that provides a means to fill this knowledge gap by assembling the hierarchy of interactions among genes, proteins, and other intermediate phenotypes that manifest as phenotypic variation...
March 2011: Journal of Nutrition
B Roger, J Papin, P Vacher, M Raoux, A Mulot, M Dubois, J Kerr-Conte, B H Voy, F Pattou, G Charpentier, J-C Jonas, N Moustaïd-Moussa, J Lang
AIMS/HYPOTHESIS: Glucose and incretins regulate beta cell function, gene expression and insulin exocytosis via calcium and cAMP. Prolonged exposure to elevated glucose (also termed glucotoxicity) disturbs calcium homeostasis, but little is known about cAMP signalling. We therefore investigated long-term effects of glucose on this pathway with special regard to the incretin glucagon-like peptide 1 (GLP-1). METHODS: We exposed INS-1E cells and rat or human islets to different levels of glucose for 3 days and determined functional responses in terms of second messengers (cAMP, Ca(2+)), transcription profiles, activation of cAMP-responsive element (CRE) and secretion by measuring membrane capacitance...
February 2011: Diabetologia
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