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Emily R Cope, Brynn H Voy, Brian K Whitlock, Meg Staton, Thomas Lane, Jack Davitt, J Travis Mulliniks
To identify molecular pathways of metabolic imbalances and reproduction, 10 castrated male sheep were randomly assigned to be injected into the lateral ventricle through intracerebroventricular cannulas with 1 mL of β-hydroxybutyric acid sodium salt solution (BHB; 12,800 µmol/L) or saline solution (CON; 0.9% NaCl). Approximately two hours post injection, sheep were humanely euthanized, and hypothalamus and pituitary tissues were harvested for transcriptome characterization using RNA-sequencing. Ribonucleic acid (RNA) was extracted from the hypothalamus and pituitary and sequenced at a high depth (Hypothalamus: 468,912,732 reads; Pituitary: 515,106,092 reads) using the Illumina Hi-Seq 2500 platform and aligned to Bos taurus and Ovis aries genomes...
April 6, 2018: Physiological Genomics
Aram A Namavar, Voy Marczynski, Young M Choi, Jashin J Wu
This study provides rankings of individual US dermatology residency programs based on a number of factors, including annual amount of National Institutes of Health (NIH) funding received in 2014, number of publications by full-time faculty members in 2014, and number of faculty lectures given at 5 annual society meetings. The overall ranking of the top 20 US dermatology residency programs is given as well as the top 5 programs for the factors most reflective of academic achievement.
February 2018: Cutis; Cutaneous Medicine for the Practitioner
Rebecca R Payton, Louisa A Rispoli, Kimberly A Nagle, Cedric Gondro, Arnold M Saxton, Brynn H Voy, J Lannett Edwards
Hyperthermia during estrus has direct consequences on the maturing oocyte that carries over to the resultant embryo to compromise its ability to continue in development. Because early embryonic development is reliant upon maternal transcripts and other ooplasmic components, we examined impact of heat stress on bovine oocyte transcripts using microarray. Oocytes were matured at 38.5ºC for 24 h or 41.0ºC for the first 12 h of in vitro maturation; 38.5ºC thereafter. Transcriptome profile was performed on total (adenylated + deadenylated) RNA and polyadenylated mRNA populations...
March 18, 2018: Journal of Reproduction and Development
Ann Wells, William T Barrington, Stephen Dearth, Amanda May, David W Threadgill, Shawn R Campagna, Brynn H Voy
Dietary intervention is commonly used for weight loss or to improve health, as diet-induced obesity increases the risk of developing type 2 diabetes, hypertension, cardiovascular disease, stroke, osteoarthritis, and certain cancers. Various dietary patterns are associated with effects on health, yet little is known about the effects of diet at the tissue level. Using untargeted metabolomics, this study aimed to identify changes in water-soluble metabolites in C57BL/6J males and females fed one of five diets (Japanese, ketogenic, Mediterranean, American, and standard mouse chow) for 7 months...
March 2, 2018: Journal of Proteome Research
Eric D Tague, Allen K Bourdon, Amber MacDonald, Maggie S Lookadoo, Edward D Kim, Wesley M White, Paul D Terry, Shawn R Campagna, Brynn H Voy, Jay Whelan
Zyflamend is a highly controlled blend of 10 herbal extracts that synergistically impact multiple cell signaling pathways with anticancer and anti-inflammatory properties. More recently, its effects were shown to also modify cellular energetics, for example, activation of fatty acid oxidation and inhibition of lipogenesis. However, its general metabolic effects in vivo have yet to be explored. The objective of this study was to characterize the tissue specific metabolomes in response to supplementation of Zyflamend in mice, with a comparison of equivalent metabolomics data generated in plasma from humans supplemented with Zyflamend...
March 2018: Journal of Medicinal Food
William T Barrington, Phillip Wulfridge, Ann E Wells, Carolina Mantilla Rojas, Selene Y F Howe, Amie Perry, Kunjie Hua, Michael A Pellizzon, Kasper D Hansen, Brynn H Voy, Brian J Bennett, Daniel Pomp, Andrew P Feinberg, David W Threadgill
The incidence of diet-induced metabolic disease has soared over the last half-century, despite national efforts to improve health through universal dietary recommendations. Studies comparing dietary patterns of populations with health outcomes have historically provided the basis for healthy diet recommendations. However, evidence that population-level diet responses are reliable indicators of responses across individuals is lacking. This study investigated how genetic differences influence health responses to several popular diets in mice, which are similar to humans in genetic composition and the propensity to develop metabolic disease, but enable precise genetic and environmental control...
January 2018: Genetics
Ronique C Beckford, Sarah J Howard, Suchita Das, Abigail T Farmer, Shawn R Campagna, Jiali Yu, Robert L Hettich, Jeanna L Wilson, Brynn H Voy
Maternal intake of eicosapentaenoic acid (EPA; 20:5 n-3) and docosahexaenoic acid (22:6 n-3) has been associated with reduced adiposity in children, suggesting the possibility to program adipose development through dietary fatty acids before birth. This study determined if enriching the maternal diet in fish oil, the primary source of EPA and DHA, affected adipose development in offspring. Broiler chickens were used because they are obesity-prone, and because fatty acids provided to the embryo can be manipulated through the hen diet...
October 13, 2017: Scientific Reports
Antja-Voy Hartley, Matthew Martin, Tao Lu
No abstract text is available yet for this article.
September 20, 2017: Aging
Emmanuelle Torchon, Rodney Ray, Matthew W Hulver, Ryan P McMillan, Brynn H Voy
Upregulating the fatty acid oxidation capacity of white adipose tissue in mice protects against diet-induced obesity, inflammation and insulin resistance. Part of this capacity results from induction of brown-like adipocytes within classical white depots, making it difficult to determine the oxidative contribution of the more abundant white adipocytes. Avian genomes lack a gene for uncoupling protein 1 and are devoid of brown adipose cells, making them a useful model in which to study white adipocyte metabolism in vivo...
January 2, 2017: Adipocyte
Matthew Martin, Laiqing Hua, Benlian Wang, Han Wei, Lakshmi Prabhu, Antja-Voy Hartley, Guanglong Jiang, Yunlong Liu, Tao Lu
Y box protein 1 (YBX1) is a well known oncoprotein that has tumor-promoting functions. YBX1 is widely considered to be an attractive therapeutic target in cancer. To develop novel therapeutics to target YBX1, it is of great importance to understand how YBX1 is finely regulated in cancer. Previously, we have shown that YBX1 could function as a tumor promoter through phosphorylation of its Ser-165 residue, leading to the activation of the NF-κB signaling pathway (1). In this study, using mass spectrometry analysis, we discovered a distinct phosphorylation site, Ser-176, on YBX1...
February 24, 2017: Journal of Biological Chemistry
Lakshmi Prabhu, Rasika Mundade, Benlian Wang, Han Wei, Antja-Voy Hartley, Matthew Martin, Kyle McElyea, Constance J Temm, George Sandusky, Yunlong Liu, Tao Lu
Y-box binding protein 1 [YBX1] is a multifunctional protein known to facilitate many of the hallmarks of cancer. Elevated levels of YBX1 protein are highly correlated with cancer progression, making it an excellent marker in cancer. The connection between YBX1 and the important nuclear factor κB [NF-κB] has never been reported. Here, we show that overexpression of wild type YBX1 [WT-YBX1] activates NF-κB, suggesting that YBX1 is a potential NF-κB activator. Furthermore, using mass spectrometry analysis we identified novel phosphorylation of serine 165 [S165] on YBX1...
October 6, 2015: Oncotarget
Benlian Wang, Han Wei, Lakshmi Prabhu, Wei Zhao, Matthew Martin, Antja-Voy Hartley, Tao Lu
Nuclear factor κB (NF-κB) is a central coordinator in immune and inflammatory responses. Constitutive NF-κB is often found in some types of cancers, contributing to oncogenesis and tumor progression. Therefore, knowing how NF-κB is regulated is important for its therapeutic control. Post-translational modification of the p65 subunit of NF-κB is a well known approach for its regulation. Here, we reported that in response to interleukin 1β, the p65 subunit of NF-κB is phosphorylated on the novel serine 316...
August 14, 2015: Journal of Biological Chemistry
M V Dodson, R E Allen, M Du, W G Bergen, S G Velleman, S P Poulos, M Fernyhough-Culver, M B Wheeler, S K Duckett, M R I Young, B H Voy, Z Jiang, G J Hausman
If one were to compare today's animal growth research to research from a mere 50 yr ago, one would see programs with few similarities. The evolution of this research from whole-animal through cell-based and finally molecular and genomic studies has been enhanced by the identification, isolation, and in vitro evaluation of adipose- and muscle-derived stem cells. This paper will highlight the struggles and the milestones that make this evolving area of research what it is today. The contribution of adipose and muscle stem cell research to development and growth, tissue regeneration, and final carcass composition are reviewed...
February 2015: Journal of Animal Science
Jörg-Detlef Drenckhahn, Jette Strasen, Kirsten Heinecke, Patrick Langner, Kom Voy Yin, Friederike Skole, Maria Hennig, Bastian Spallek, Robert Fischer, Florian Blaschke, Arnd Heuser, Timothy C Cox, Mary Jane Black, Ludwig Thierfelder
AIMS: Foetal growth has been proposed to influence cardiovascular health in adulthood, a process referred to as foetal programming. Indeed, intrauterine growth restriction in animal models alters heart size and cardiomyocyte number in the perinatal period, yet the consequences for the adult or challenged heart are largely unknown. The aim of this study was to elucidate postnatal myocardial growth pattern, left ventricular function, and stress response in the adult heart after neonatal cardiac hypoplasia in mice...
April 1, 2015: Cardiovascular Research
Bo Ji, Jesse L Middleton, Ben Ernest, Arnold M Saxton, Susan J Lamont, Shawn R Campagna, Brynn H Voy
Domestic broiler chickens rapidly accumulate fat and are naturally hyperglycemic and insulin resistant, making them an attractive model for studies of human obesity. We previously demonstrated that short-term (5 h) fasting rapidly upregulates pathways of fatty acid oxidation in broiler chickens and proposed that activation of these pathways may promote leanness. The objective of the current study was to characterize adipose tissue from relatively lean and fatty lines of chickens and determine if heritable leanness in chickens is associated with activation of some of the same pathways induced by fasting...
May 1, 2014: Physiological Genomics
Eugene Kolker, Vural Özdemir, Lennart Martens, William Hancock, Gordon Anderson, Nathaniel Anderson, Sukru Aynacioglu, Ancha Baranova, Shawn R Campagna, Rui Chen, John Choiniere, Stephen P Dearth, Wu-Chun Feng, Lynnette Ferguson, Geoffrey Fox, Dmitrij Frishman, Robert Grossman, Allison Heath, Roger Higdon, Mara H Hutz, Imre Janko, Lihua Jiang, Sanjay Joshi, Alexander Kel, Joseph W Kemnitz, Isaac S Kohane, Natali Kolker, Doron Lancet, Elaine Lee, Weizhong Li, Andrey Lisitsa, Adrian Llerena, Courtney Macnealy-Koch, Jean-Claude Marshall, Paola Masuzzo, Amanda May, George Mias, Matthew Monroe, Elizabeth Montague, Sean Mooney, Alexey Nesvizhskii, Santosh Noronha, Gilbert Omenn, Harsha Rajasimha, Preveen Ramamoorthy, Jerry Sheehan, Larry Smarr, Charles V Smith, Todd Smith, Michael Snyder, Srikanth Rapole, Sanjeeva Srivastava, Larissa Stanberry, Elizabeth Stewart, Stefano Toppo, Peter Uetz, Kenneth Verheggen, Brynn H Voy, Louise Warnich, Steven W Wilhelm, Gregory Yandl
Biological processes are fundamentally driven by complex interactions between biomolecules. Integrated high-throughput omics studies enable multifaceted views of cells, organisms, or their communities. With the advent of new post-genomics technologies, omics studies are becoming increasingly prevalent; yet the full impact of these studies can only be realized through data harmonization, sharing, meta-analysis, and integrated research. These essential steps require consistent generation, capture, and distribution of metadata...
January 2014: Omics: a Journal of Integrative Biology
Jane Morris, Ashley V Simpson, Steven James Voy
INTRODUCTION: Inpatient treatment for eating disorders is a scarce, expensive resource. We aimed to examine length of stay (LOS) in specialist Scottish inpatient units and to identify relationships between LOS, outcome measures and other factors. METHOD: Audit of 206 admissions (89 adolescents and 117 adults) between 2009 and 2011 to all seven inpatient units in Scotland that specialize in the treatment of eating disorders. Data was collected retrospectively from electronic database and patient case files...
January 2015: Clinical Psychology & Psychotherapy
Xinping Huang, Antja-Voy Hartley, Yishi Yin, Jeremy H Herskowitz, James J Lah, Kerry J Ressler
The adeno-associated virus (AAV) is one of the most useful viral vectors for gene delivery for both in vivo and in vitro applications. A variety of methods have been established to produce and characterize recombinant AAV (rAAV) vectors; however most methods are quite cumbersome and obtaining consistently high titer can be problematic. This protocol describes a triple-plasmid co-transfection approach with 25 kDa linear polyethylenimine (PEI) in 293 T cells for the production of AAV serotype 2. Seventy-two hours post-transfection, supernatant and cells were harvested and purified by a discontinuous iodixanol density gradient ultracentrifugation, then dialyzed and concentrated with an Amicon 15 100,000 MWCO concentration unit...
November 2013: Journal of Virological Methods
Sarah J Fletcher, Nishan S Kalupahana, Morvarid Soltani-Bejnood, Jung Han Kim, Arnold M Saxton, David H Wasserman, Bart De Taeye, Brynn H Voy, Annie Quignard-Boulange, Naima Moustaid-Moussa
Numerous animal and clinical investigations have pointed to a potential role of the renin-angiotensin system (RAS) in the development of insulin resistance and diabetes in conditions of expanded fat mass. However, the mechanisms underlying this association remain unclear. We used a transgenic mouse model overexpressing renin in the liver (RenTgMK) to examine the effects of chronic activation of RAS on adiposity and insulin sensitivity. Hepatic overexpression of renin resulted in constitutively elevated plasma angiotensin II (four- to six-fold increase vs...
2012: Frontiers in Endocrinology
Ben Ernest, Jessica R Gooding, Shawn R Campagna, Arnold M Saxton, Brynn H Voy
BACKGROUND: Metabolomics is an emerging high-throughput approach to systems biology, but data analysis tools are lacking compared to other systems level disciplines such as transcriptomics and proteomics. Metabolomic data analysis requires a normalization step to remove systematic effects of confounding variables on metabolite measurements. Current tools may not correctly normalize every metabolite when the relationships between each metabolite quantity and fixed-effect confounding variables are different, or for the effects of random-effect confounding variables...
2012: BMC Research Notes
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