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https://www.readbyqxmd.com/read/28506869/model-based-clinical-dose-optimization-for-phenobarbital-in-neonates-an-illustration-of-the-importance-of-data-sharing-and-external-validation
#1
Swantje Völler, Robert B Flint, Leo M Stolk, Pieter L J Degraeuwe, Sinno H P Simons, Paula Pokorna, David M Burger, Ronald de Groot, Dick Tibboel, Catherijne A J Knibbe
BACKGROUND: Particularly in the pediatric clinical pharmacology field, data-sharing offers the possibility of making the most of all available data. In this study, we utilize previously collected therapeutic drug monitoring (TDM) data of term and preterm newborns to develop a population pharmacokinetic model for phenobarbital. We externally validate the model using prospective phenobarbital data from an ongoing pharmacokinetic study in preterm neonates. METHODS: TDM data from 53 neonates (gestational age (GA): 37 (24-42) weeks, bodyweight: 2...
May 12, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28504473/dynamic-cooperative-glycan-assembly-blocks-the-binding-of-bacterial-lectins-to-epithelial-cells
#2
Takuya Machida, Alexandre Novoa, Émilie Gillon, Shuangshuang Zheng, Julie Claudinon, Thorsten Eierhoff, Anne Imberty, Winfried Römer, Nicolas Winssinger
Pathogens frequently rely on lectins for adhesion and cellular entry into the host. Since these interactions typically result from multimeric binding of lectins to cell-surface glycans, novel therapeutic strategies are being developed with the use of glycomimetics as competitors of such interactions. Herein we study the benefit of nucleic acid based oligomeric assemblies with PNA-fucose conjugates. We demonstrate that the interactions of a lectin with epithelial cells can be inhibited with conjugates that do not form stable assemblies in solution but benefit from cooperativity between ligand-protein interactions and PNA hybridization to achieve high affinity...
May 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28496969/synthetic-approaches-to-nucleopeptides-containing-all-four-nucleobases-and-nucleic-acid-binding-studies-on-a-mixed-sequence-nucleo-oligolysine
#3
Giovanni N Roviello, Domenica Musumeci
In this article we describe two solid-phase synthetic routes to obtain a nucleo-oligolysine α-peptide containing all four natural nucleobases. The first one is based on the oligomerization of the nucleobase-containing monomers, easily synthesized as herein described. The second strategy has the advantage of avoiding the solution synthesis of the monomeric building blocks, leading to the final nucleopeptide by direct solid-phase couplings of the suitably protected nucleobases with the free amino groups on the growing peptide chain still anchored to the resin...
July 16, 2016: RSC Advances
https://www.readbyqxmd.com/read/28495410/a-case-and-control-multisite-positive-controlled-prospective-study-of-the-safety-and-effectiveness-of-immediate-inferior-alveolar-nerve-processed-nerve-allograft-reconstruction-with-ablation-of-the-mandible-for-benign-pathology
#4
John R Zuniga, Fayette Williams, Daniel Petrisor
PURPOSE: This study determined whether immediate reconstruction of the inferior alveolar nerve with a long (>4.5 cm) processed nerve allograft (PNA) in conjunction with simultaneous ablation and reconstruction of the mandible would be effective in safely restoring subjective sensation and achieving functional sensory recovery. MATERIALS AND METHODS: Patients (5 to 70 yr old) requiring resection of the unilateral or bilateral mandible for benign pathology were included...
April 13, 2017: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28485004/sialic-acid-expression-in-human-fetal-skeletal-muscle-during-limb-early-myogenesis
#5
Mirca Marini, Erica Sarchielli, Giorgia Donata Zappoli Thyrion, Stefano Ambrosini, Eleonora Sgambati
Investigations on animal models demonstrated that changes of sialic acid (SA) expression, particularly the polymeric form, in the skeletal muscle during embryonic and post-natal development seem to be related to muscle differentiation and functionality onset. The aim of this study was to evaluate the monomeric and polymeric SA expression in human skeletal muscle during early stages of fetal development, when important morphofunctional events occur. Specimens of fetal skeletal muscle from limb, between 9 and 12 weeks of gestation (wg), were obtained from 19 pregnant women...
May 9, 2017: Histology and Histopathology
https://www.readbyqxmd.com/read/28479722/influence-of-capsaicin-on-inflammatory-cytokines-induced-by-lipopolysaccharide-in-myoblast-cells-under-in-vitro-environment
#6
Ke Shang, Touseef Amna, Musarat Amina, Nawal M Al-Musayeib, Salem S Al-Deyab, Inho Hwang
BACKGROUND: ellular damage initiated by reactive oxygen species (ROS) is the main cause of numerous severe diseases and therefore for this reason, the natural antioxidants have note worthy significance in human health. Capsaicin possesses noteworthy analgesic and anti-inflammatory properties. It also possesses healing effects for treatment of arthritis, diabetic neuropathy, gastric lesions, and cardiac excitability that is why it is incorporated in creams and gels. OBJECTIVE: The present study was carried out to estimate the in vitro antioxidant and ROS scavenging activities of capsaicin against muscle precursor cells...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28469617/exploration-of-using-antisense-peptide-nucleic-acid-pna-cell-penetrating-peptide-cpp-as-a-novel-bactericide-against-fire-blight-pathogen-erwinia-amylovora
#7
Ravi R Patel, George W Sundin, Ching-Hong Yang, Jie Wang, Regan B Huntley, Xiaochen Yuan, Quan Zeng
Erwinia amylovora is a Gram-negative bacterial plant pathogen in the family Enterobacteriaceae and is the causal agent of fire blight, a devastating disease of apple and pear. Fire blight is traditionally managed by the application of the antibiotic streptomycin during bloom, but this strategy has been challenged by the development and spread of streptomycin resistance. Thus, there is an urgent need for effective, specific, and sustainable control alternatives for fire blight. Antisense antimicrobials are oligomers of nucleic acid homologs with antisense sequence of essential genes in bacteria...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28460983/protein-network-analysis-a-new-approach-for-quantifying-wheat-dough-microstructure
#8
Isabelle Bernklau, Lars Lucas, Mario Jekle, Thomas Becker
Clarification of wheat dough functionalities by visualizing the protein microstructure demands a precise image analysis, which is still challenging. Thus, a novel method for quantifying dough microstructure called protein network analysis (PNA) was established in this study. Hereby, absolute morphological attributes such as junctions' density, branching rate, end-point rate, and lacunarity quantify and characterize the strength of a network. The method was validated in a large range of varying microstructural shapes by increasing the bulk water concentration...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28453005/a-p-nitroaniline-redox-active-solid-state-electrolyte-for-battery-like-electrochemical-capacitive-energy-storage-combined-with-an-asymmetric-supercapacitor-based-on-metal-oxide-functionalized-%C3%AE-polytype-porous-silicon-carbide-electrodes
#9
Myeongjin Kim, Jeeyoung Yoo, Jooheon Kim
A unique redox active flexible solid-state asymmetric supercapacitor with ultra-high capacitance and energy density was fabricated using a composite comprising MgCo2O4 nanoneedles and micro and mesoporous silicon carbide flakes (SiCF) (SiCF/MgCo2O4) as the positive electrode material. Due to the synergistic effect of the two materials, this hybrid electrode has a high specific capacitance of 516.7 F g(-1) at a scan rate of 5 mV s(-1) in a 1 M KOH aqueous electrolyte. To obtain a reasonable matching of positive and negative electrode pairs, a composite of Fe3O4 nanoparticles and SiCF (SiCF/Fe3O4) was synthesized for use as a negative electrode material, which shows a high capacitance of 423...
April 28, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28448906/variation-in-the-toxicity-of-sediment-associated-substituted-phenylamine-antioxidants-to-an-epibenthic-hyalella-azteca-and-endobenthic-tubifex-tubifex-invertebrate
#10
R S Prosser, A J Bartlett, D Milani, E A M Holman, H Ikert, D Schissler, J Toito, J L Parrott, P L Gillis, V K Balakrishnan
Substituted phenylamine antioxidants (SPAs) are produced in relatively high volumes and used in a range of applications (e.g., rubber, polyurethane); however, little is known about their toxicity to aquatic biota. Therefore, current study examined the effects of chronic exposure (28 d) to four sediment-associated SPAs on epibenthic (Hyalella azteca) and endobenthic (Tubifex tubifex) organisms. In addition, acute (96-h), water-only exposures were conducted with H. azteca. Mortality, growth and biomass production were assessed in juvenile H...
April 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28448556/a-highly-specific-and-sensitive-massive-parallel-sequencer-based-test-for-somatic-mutations-in-non-small-cell-lung-cancer
#11
Yoshiaki Inoue, Jun Shiihara, Hitoshi Miyazawa, Hiromitsu Ohta, Megumi Higo, Yoshiaki Nagai, Kunihiko Kobayashi, Yasuo Saijo, Masanori Tsuchida, Mitsuo Nakayama, Koichi Hagiwara
Molecular targeting therapy for non-small cell lung cancer (NSCLC) has clarified the importance of mutation testing when selecting treatment regimens. As a result, multiple-gene mutation tests are urgently needed. We developed a next-generation sequencer (NGS)-based, multi-gene test named the MINtS for investigating driver mutations in both cytological specimens and snap-frozen tissue samples. The MINtS was used to investigate the EGFR, KRAS, BRAF genes from DNA, and the ERBB2, and the ALK, ROS1, and RET fusion genes from RNA...
2017: PloS One
https://www.readbyqxmd.com/read/28438316/multiple-modes-of-capillary-electrophoresis-applied-in-peptide-nucleic-acid-related-study
#12
Xiaoqian Wang, Youhao Hu, Feng Qu, Rizwan Ullah Khan
Peptide Nucleic Acid (PNA) is a nucleic acid analogue, whose neutrally charged and hydrophobic backbone makes it more stable in vivo, so it might act as a potentially better protein probe as compared to aptamer. Currently the investigation of PNA and protein interaction is scarce. In this research, multiple modes of capillary electrophoresis were established and applied for PNA characterization and its interaction with ssDNA and protein. A 15-mer PNA having the same nucleobase sequence as 15-mer anti-thrombin DNA aptamer was chosen as PNA model for this study, its pI (7...
April 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28435017/association-of-paraoxonase-1-and-oxidative-stress-with-acute-kidney-injury-in-premature-asphyxiated-neonates
#13
Jasmina Ivanišević, Jelena Kotur-Stevuljević, Aleksandra Stefanović, Milica Miljković, Zorana Jelić-Ivanović, Biljana Pejović, Amira Peco-Antić
OBJECTIVES: Acute kidney injury (AKI) is defined as a decrease in glomerular filtration rate with an increase in serum creatinine (sCr). Perinatal asphyxia (PNA) may be etiological factor for AKI with oxidative stress also implicated. Paraoxonase 1 (PON1) activity has been reported to be decreased in renal disease. The aim of our study was to evaluate paraoxonase 1 (PON1) activity and oxidative stress during the first hours and first days of life and to determine if these parameters could discriminate neonates having AKI from those who do not...
April 20, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28434220/effects-of-the-backbone-and-chemical-linker-on-the-molecular-conductance-of-nucleic-acid-duplexes
#14
Edward Beall, Selma Ulku, Chaoren Liu, Emil Wierzbinski, Yuqi Zhang, Yookyung Bae, Peng Zhang, Catalina Achim, David N Beratan, David H Waldeck
Scanning tunneling microscope break junction measurements are used to examine how the molecular conductance of nucleic acids depends on the composition of their backbone and the linker group to the electrodes. Molecular conductances of 10 base pair long homoduplexes of DNA, aeg-PNA, γ-PNA, and a heteroduplex of DNA/aeg-PNA with identical nucleobase sequence were measured. The molecular conductance was found to vary by 12 to 13 times with the change in backbone. Computational studies show that the molecular conductance differences between nucleic acids of different backbones correlate with differences in backbone structural flexibility...
May 4, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28431414/intratracheal-instillation-of-neutrophils-rescues-bacterial-overgrowth-initiated-by-trauma-damage-associated-molecular-patterns
#15
Kiyoshi Itagaki, Ingred Riça, Jing Zhang, Dave Gallo, Melissa DePrato, Leo E Otterbein, Carl J Hauser
BACKGROUND: Nosocomial pneumonias are common in trauma patients and so interventions to prevent and treat nosocomial pneumonia may improve outcomes. Our prior work strongly suggests that tissue injury predisposes to infections like nosocomial pneumonia because mitochondrial debris originating from injured cells contains damage-associated molecular patterns that can reduce neutrophil (PMN) migration into the airway and diminish PMN function in response to bacterial inoculation of the airway...
May 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28429810/cisplatin-directed-coordination-crosslinking-nanogels-with-thermo-ph-sensitive-triblock-polymers-improvement-on-chemotherapic-efficacy-via-sustained-release-and-drug-retention
#16
Hao Zhao, Jiabao Xu, Jiangshan Wan, Shinan Geng, Han Li, Xiaole Peng, Qianwen Fu, Ming He, Yanbing Zhao, Xiangliang Yang
To realize the sustained release and long-term intratumoural retention of water-soluble cisplatin, thermo/pH-sensitive cisplatin-directed coordination-crosslinking nanogels (Pt-PNA) were developed via the coordination bonds of Pt-carboxyl groups. As the coordination ratio (CR) of the Pt-carboxyl bonds increased from 5% to 35%, the sizes of the Pt-PNA nanogels decreased from 999 nm to 167 nm, and their zeta potentials increased from -35 mV to -13 mV. Only through a simple mixing of cisplatin and PNAs, the entrapment efficiencies (EEs) of the Pt-PNA nanogels reached near 100% (>90%), and the drug-loading amounts (DLs) of cisplatin could achieve up to 25...
May 11, 2017: Nanoscale
https://www.readbyqxmd.com/read/28423373/distribution-of-interstitial-telomeric-sequences-in-primates-and-the-pygmy-tree-shrew-scandentia
#17
Sofia Mazzoleni, Odessa Schillaci, Luca Sineo, Francesca Dumas
It has been hypothesized that interstitial telomeric sequences (ITSs), i.e., repeated telomeric DNA sequences found at intrachromosomal sites in many vertebrates, could be correlated to chromosomal rearrangements and plasticity. To test this hypothesis, we hybridized a telomeric PNA probe through FISH on representative species of 2 primate infraorders, Strepsirrhini (Lemur catta, Otolemur garnettii, Nycticebus coucang) and Catarrhini (Erythrocebus patas, Cercopithecus petaurista, Chlorocebus aethiops, Colobus guereza), as well as on 1 species of the order Scandentia, Tupaia minor, used as an outgroup for primates in phylogenetic reconstructions...
April 20, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/28420874/therapeutic-targeting-using-tumor-specific-peptides-inhibits-long-non-coding-rna-hotair-activity-in-ovarian-and-breast-cancer
#18
Ali R Özeş, Yinu Wang, Xingyue Zong, Fang Fang, Jay Pilrose, Kenneth P Nephew
Long non-coding RNAs (lncRNAs) play key roles in human diseases, including cancer. Functional studies of the lncRNA HOTAIR (HOX transcript antisense RNA) provide compelling evidence for therapeutic targeting of HOTAIR in cancer, but targeting lncRNAs in vivo has proven to be difficult. In the current study, we describe a peptide nucleic acids (PNA)-based approach to block the ability of HOTAIR to interact with EZH2 and subsequently inhibit HOTAIR-EZH2 activity and resensitize resistant ovarian tumors to platinum...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418159/toxicity-of-sediment-associated-substituted-phenylamine-antioxidants-on-the-early-life-stages-of-pimephales-promelas-and-a-characterization-of-effects-on-freshwater-organisms
#19
Ryan S Prosser, Joanne L Parrott, Melissa Galicia, Kallie Shires, Cheryl Sullivan, John Toito, Adrienne J Bartlett, Danielle Milani, Patty L Gillis, Vimal K Balakrishnan
Substituted phenylamine antioxidants (SPAs) are high production volume chemicals that are incorporated into a variety of commercial products (e.g., polymers, dyes, lubricants). There are little data on chronic toxicity of SPAs to fish and no data on the toxicity of SPAs to the early-life stages of fish. The physicochemical properties of SPAs would suggest that if they were to enter an aquatic ecosystem they would partition into sediment. Therefore, the current study focused on investigating the chronic effect of sediment-associated SPAs to the early-life stages of the fathead minnow (Pimephales promelas)...
April 18, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28414202/recent-advances-in-peptide-nucleic-acid-for-cancer-bionanotechnology
#20
REVIEW
Jun-Chen Wu, Qing-Chun Meng, Hong-Mei Ren, Hong-Tao Wang, Jie Wu, Qi Wang
Peptide nucleic acid (PNA) is an oligomer, in which the phosphate backbone has been replaced by a pseudopeptide backbone that is meant to mimic DNA. Peptide nucleic acids are of the utmost importance in the biomedical field because of their ability to hybridize with neutral nucleic acids and their special chemical and biological properties. In recent years, PNAs have emerged in nanobiotechnology for cancer diagnosis and therapy due to their high affinity and sequence selectivity toward corresponding DNA and RNA...
April 17, 2017: Acta Pharmacologica Sinica
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