keyword
MENU ▼
Read by QxMD icon Read
search

Pna

keyword
https://www.readbyqxmd.com/read/28213139/antimycobacterial-antimicrobial-activity-experimental-ft-ir-ft-raman-nmr-uv-vis-dsc-and-dft-transition-state-chemical-reactivity-nbo-nlo
#1
Poonam Rawat, R N Singh, Alok Ranjan, Sartaj Ahmad, Rajat Saxena
As part of a study of pyrrole hydrazone, we have investigated quantum chemical calculations, molecular geometry, relative energy, vibrational properties and antimycobacterial/antimicrobial activity of pyrrole-2-carboxaldehyde isonicotinyl hydrazone (PCINH), by applying the density functional theory (DFT) and Hartree Fock (HF). Good reproduction of experimental values is obtained and with small percentage error in majority of the cases in comparison to theoretical result (DFT). The experimental FT-IR and Raman wavenumbers were compared with the respective theoretical values obtained from DFT calculations and found to agree well...
February 11, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28211525/focus-on-pna-flexibility-and-rna-binding-using-molecular-dynamics-and-metadynamics
#2
Massimiliano Donato Verona, Vincenzo Verdolino, Ferruccio Palazzesi, Roberto Corradini
Peptide Nucleic Acids (PNAs) can efficiently target DNA or RNA acting as chemical tools for gene regulation. Their backbone modification and functionalization is often used to increase the affinity for a particular sequence improving selectivity. The understanding of the trading forces that lead the single strand PNA to bind the DNA or RNA sequence is preparatory for any further rational design, but a clear and unique description of this process is still not complete. In this paper we report further insights into this subject, by a computational investigation aiming at the characterization of the conformations of a single strand PNA and how these can be correlated to its capability in binding DNA/RNA...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195541/assessment-of-egfr-mutation-status-using-cell-free-dna-from-bronchoalveolar-lavage-fluid
#3
Sojung Park, Jae Young Hur, Kye Young Lee, Jae Cheol Lee, Jin Kyung Rho, Sun Hwa Shin, Chang-Min Choi
BACKGROUND: Much attention has been focused on epidermal growth factor receptor (EGFR) mutation testing since the introduction of EGFR-tyrosine kinase inhibitors have improved survival in EGFR-positive lung cancer patients. Liquid biopsy using circulating tumor cells or cell-free DNA (cfDNA) has enabled less invasive testing, but requires a highly sensitive method. To date, liquid biopsy using bronchoalveolar lavage (BAL) fluid has rarely been used. METHODS: From 20 patients with lung adenocarcinoma, we isolated cfDNA from 20 samples of cell-free BAL fluid and 19 cell-free bronchial washing samples...
February 14, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28167114/an-antisense-peptide-nucleic-acid-against-pseudomonas-aeruginosa-inhibiting-bacterial-induced-inflammatory-responses-in-the-cystic-fibrosis-ib3-1-cellular-model-system
#4
Giulia Montagner, Valentino Bezzerri, Giulio Cabrini, Enrica Fabbri, Monica Borgatti, Ilaria Lampronti, Alessia Finotti, Peter E Nielsen, Roberto Gambari
Discovery of novel antimicrobial agents against Pseudomonas aeruginosa able to inhibit bacterial growth as well as the resulting inflammatory response is a key goal in cystic fibrosis research. We report in this paper that a peptide nucleic acid (PNA3969) targeting the translation initiation region of the essential acpP gene of P. aeruginosa, and previously shown to inhibit bacterial growth, concomitantly also strongly inhibits PAO1 induced up-regulation of the pro-inflammatory markers IL-8, IL-6, G-CSF, IFN-γ, IP-10, MCP-1 and TNF-α in IB3-1 cystic fibrosis cells infected by P...
February 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28166424/lectin-histochemistry-for-detecting-cadmium-induced-changes-in-the-glycosylation-pattern-of-rat-placenta
#5
M C Díaz, N V González, C N Zanuzzi, R Najle, C G Barbeito
Cadmium (Cd) is an industrial and environmental pollutant that produces toxic effects on gametogenesis, pre- and post-implantation embryos, and the placenta. Because the effects of acute Cd intoxication on the placenta are not well understood, we investigated changes in its glycosylated components in Cd treated dams at days 4, 7, 10 and 15 of gestation using lectin histochemistry. CdCl2 was administered to pregnant rats; control animals received sterile normal saline. Placentas were processed for DBA, Con A, SBA, PNA, UEA-I, RCA-I and WGA lectin histochemistry to evaluate changes in the carbohydrate pattern of the placenta that might modify cell interactions and contribute to embryonic alterations...
February 6, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28161501/development-of-highly-stable-binder-free-chemical-sensor-electrodes-for-p-nitroaniline-detection
#6
Rafiq Ahmad, Nirmalya Tripathy, Min-Sang Ahn, Yoon-Bong Hahn
Herein, pre-seeded fluorine doped tin oxide (FTO) glass substrates were used as an electrode for zinc oxide nanorods (ZnO NRs) growth by a low-temperature solution route in order to fabricate binder-free high-sensitive chemical sensor. The vertically-grown ZnO NRs exhibited a more favorable active morphology and improved sensing properties for p-nitroaniline (pNA) detection. On investigation with different concentrations of pNA, the ZnO NRs/FTO electrode showed an excellent sensitivity (10.18μAμM(-1)cm(-2)) and low detection limit (0...
May 15, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28159694/molecular-cloning-characterization-and-expression-analysis-of-caspase-3-from-the-oriental-river-prawn-macrobrachium-nipponense-when-exposed-to-acute-hypoxia-and-reoxygenation
#7
Shengming Sun, Fujun Xuan, Hongtuo Fu, Jian Zhu, Xianping Ge, Xugan Wu
Caspases are present in the cytosol as inactive proenzymes but become activated when apoptosis is initiated, playing an essential role at various stages of the process. In this study, a caspase-3 (Mncaspase-3c) was cloned from gill of the oriental river prawn Macrobrachium nipponense by reverse-transcription polymerase chain reaction and rapid amplification of cDNA ends, and its properties were characterized. The 1730-bp cDNA contained an open reading frame of 1566 bp, a 123-bp 5'-untranslated region (UTR), and a 41-bp 3'-UTR containing a poly(A) tail...
January 31, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28149160/immune-characteristics-study-of-ag490-a-signal-pathway-inhibitor-in-eae-model-mice
#8
Zhihua Zhao, Zhifeng Mao, Junjie Yin, Yaqing Shu, Wei Qiu, Zhengqi Lu, Xueqiang Hu
Multiple sclerosis (MS) is an autoimmune disease characterized by demyelination, axonal damage and progressive neurologic dysfunction in central nervous system (CNS). Many evidences show that B cells play an important role in the pathogenesis of MS. Follicular helper T cells (Tfh) secrete IL-21 to prompt the proliferation and differentiation of B cells in germinal center (GC) through clonal proliferation, somatic hypermutation, antibody class switching, antibody affinity maturation process. AG490 is a synthetic inhibitor to JAK-STAT signal pathway, which has been studied in inflammatory, tumor and autoimmune diseases...
February 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28144839/yeasts-identification-in-microfluidic-devices-using-peptide-nucleic-acid-fluorescence-in-situ-hybridization-pna-fish
#9
André M Ferreira, Daniela Cruz-Moreira, Laura Cerqueira, João M Miranda, Nuno F Azevedo
Peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) is a highly specific molecular method widely used for microbial identification. Nonetheless, and due to the detection limit of this technique, a time-consuming pre-enrichment step is typically required before identification. In here we have developed a lab-on-a-chip device to concentrate cell suspensions and speed up the identification process in yeasts. The PNA-FISH protocol was optimized to target Saccharomyces cerevisiae, a common yeast that is very relevant for several types of food industries...
March 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28144645/host-guest-interactions-and-controllable-capture-and-release-of-proteins-based-on-cationic-perylene-bisimides
#10
Qi Xu, Jia-Li Wang, Ya-Li Luo, Juan-Juan Li, Ke-Rang Wang, Xiao-Liu Li
A system of controllable capture and release of protein was constructed by multiple, interconnected supramolecular binding modules based on lactose modified mono-cationic perylene bisimide derivatives, cucurbit[8]uril (CB[8]), 1-adamantanamine (ADA) and peanut agglutinin (PNA) lectins.
February 1, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28139850/dna-detection-by-flow-cytometry-using-pna-modified-metal-organic-framework-particles
#11
Raquel Mejia-Ariza, Jessica Rosselli, Christian Breukers, Alex Manicardi, Leon Terstappen, Roberto Corradini, Jurriaan Huskens
A DNA sensing platform is developed by exploiting the easy surface functionalization of metal organic framework (MOF) particles and their highly parallelized fluorescence detection by flow cytometry. Two strategies were employed to functionalize the surface of MIL-88A, using either covalent or non-covalent interactions, resulting in alkyne-modified and biotin-modified MIL-88A, respectively. Covalent surface coupling of an azide-dye and the alkyne-MIL-88A was achieved by click reaction. Non-covalent streptavidin-biotin interactions were employed to link biotin-PNA to biotin-MIL-88A particles mediated by streptavidin...
January 31, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28139698/midcontinental-native-american-population-dynamics-and-late-holocene-hydroclimate-extremes
#12
Broxton W Bird, Jeremy J Wilson, William P Gilhooly Iii, Byron A Steinman, Lucas Stamps
Climate's influence on late Pre-Columbian (pre-1492 CE), maize-dependent Native American populations in the midcontinental United States (US) is poorly understood as regional paleoclimate records are sparse and/or provide conflicting perspectives. Here, we reconstruct regional changes in precipitation source and seasonality and local changes in warm-season duration and rainstorm events related to the Pacific North American pattern (PNA) using a 2100-year-long multi-proxy lake-sediment record from the midcontinental US...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28138841/high-molecular-weight-dna-enrichment-with-peptide-nucleic-acid-probes
#13
Nicholas M Murphy, Colin W Pouton, Helen R Irving
Here, we describe how peptide nucleic acid (PNA) probes can be used to enrich genomic DNA fractions to facilitate downstream analysis, such as the haplotype phasing of the isolated genomic pieces. This method enriches for polymorphic regions of fragmented chromosomes by physically separating the desired sequence and flanking regions. The PNA probes used for enrichment are novel synthetic nucleic acids with highly specific targeting and hybridization properties. Using a enrichment technique, we capture high molecular weight genomic DNA using nothing more than a simple modification to standard genomic DNA extraction from blood...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28131721/effects-of-acetylcholine-and-cholinergic-antagonists-on-the-activity-of-nucleus-of-the-solitary-tract-neurons
#14
Werner I Furuya, Eduardo Colombari, Alastair V Ferguson, Débora S A Colombari
Previously we have demonstrated that microinjection of acetylcholine (ACh) into the intermediate nucleus of the solitary tract (iNTS) induced sympatho-inhibition combined with a decrease in the phrenic nerve activity (PNA), whereas in the commissural NTS (cNTS), ACh did not change sympathetic nerve activity (SNA), but increased the PNA. In view of these demonstrated distinctive effects of ACh in different subnuclei of the NTS the current studies were undertaken to examine, using patch clamp techniques, the specific effects of ACh on the excitability of individual neurons in the NTS, as well as the neuropharmacology of these actions...
January 25, 2017: Brain Research
https://www.readbyqxmd.com/read/28129707/fluorescence-in-situ-hybridization-fish-in-the-microbiological-diagnostic-routine-laboratory-a-review
#15
Hagen Frickmann, Andreas Erich Zautner, Annette Moter, Judith Kikhney, Ralf Matthias Hagen, Henrik Stender, Sven Poppert
Early identification of microbial pathogens is essential for rational and conservative antibiotic use especially in the case of known regional resistance patterns. Here, we describe fluorescence in situ hybridization (FISH) as one of the rapid methods for easy identification of microbial pathogens, and its advantages and disadvantages for the diagnosis of pathogens in human infections in the laboratory diagnostic routine. Binding of short fluorescence-labeled DNA or nucleic acid-mimicking PNA probes to ribosomes of infectious agents with consecutive analysis by fluorescence microscopy allows identification of bacterial and eukaryotic pathogens at genus or species level...
January 27, 2017: Critical Reviews in Microbiology
https://www.readbyqxmd.com/read/28126369/neonatal-bladder-inflammation-induces-long-term-visceral-pain-and-altered-responses-of-spinal-neurons-in-adult-rats
#16
Pradeep Kannampalli, Reji Babygirija, Jiang Zhang, Michael M Poe, Guanguan Li, James M Cook, Reza Shaker, Banani Banerjee, Jyoti N Sengupta
Painful events early in life have been shown to increase the incidence of interstitial cystitis/painful bladder syndrome in adulthood. However, the intrinsic mechanism is not well studied. We previously reported that neonatal bladder inflammation causes chronic visceral hypersensitivity along with molecular disruption of spinal GABAergic system in rats. The present study investigates whether these molecular changes affect the integrative function and responses of bladder-sensitive primary afferent and spinal neurons...
January 23, 2017: Neuroscience
https://www.readbyqxmd.com/read/28124963/-evaluation-of-pna-fish-method-for-direct-identification-of-candida-species-in-blood-culture-samples-and-its-potential-impact-on-guidance-of-antifungal-therapy
#17
Özlem Doğan, Ahmet Çağkan İnkaya, Dolunay Gülmez, Ömrüm Uzun, Murat Akova, Sevtap Arıkan Akdağlı
Early antifungal therapy has a major influence on survival in candidemia. Rapid identification of the species has importance for the treatment, prediction of the species-specific primary resistance and variable antifungal susceptibility. Recently, molecular-based methods attempt to reduce the time between the positive signal of a blood culture and identification of the fungus. PNA-FISH (Peptide nucleic acid fluorescence in situ hybridization) assay distinguishes a number of frequently isolated Candida species in groups following the growth in blood culture...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28116281/dupuytren-s-disease-and-needle-aponeurotomy-rupture-of-a-deep-common-flexor-tendon-a-case-report-and-literature-review
#18
Salma Otayek, Jérôme Pierrart, Emmanuel H Masmejean
INTRODUCTION: The Percutaneous Needle Aponeurotomy (PNA) is a known and validated non-surgical treatment for Dupuytren's disease with a low rate of complications. Initially described for purely palmar forms, the indications extended to digital adhesions. Numerous surgeons remain hesitant about performing the PNA for digital treatment because of the risk of lesions in the noble structures. In literature, one complication is still rare, even non-existent, i.e. the tendon rupture. CASE PRESENTATION: We are reporting here the case of a rupture of the deep common flexor tendon, secondary to a needle aponeurotomy, for a recurring digital form in a 72-year-old woman...
July 2016: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28109443/separation-of-two-distinct-o-glycoforms-of-human-iga1-by-serial-lectin-chromatography-followed-by-mass-spectrometry-o-glycan-analysis
#19
S Lehoux, T Ju
Human immunoglobulin A1 (IgA1), which carries four to six mucin-type O-glycans (O-glycans) on its hinge region (HR), is the most abundant O-glycoprotein in plasma or serum. While normal O-glycans from hematopoietic-originated cells are core 1-based complex structures, many reports showed that the IgA1 from patients with IgA nephropathy (IgAN) carries undergalactosylated or truncated O-glycans such as the Tn antigen and its sialylated version the SialylTn (STn) antigen on the HR. Yet, there is still a debate whether Tn/STn on the HR of IgA1 is specific to the IgA1 from patients with IgAN since these antigens have also been seen in serum IgA1 of healthy individuals...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28106939/maximizing-output-in-rna-programmed-peptidyl-transfer-reactions
#20
Margherita Di Pisa, Anett Hauser, Oliver Seitz
A chemical reaction that is triggered by specific RNA molecules may provide opportunities for the design of artificial feedback loops. We envision a peptidyl transfer reaction, in which mRNA encoding for antiapoptotic protein would instruct the synthesis of apoptosis-inducing peptides. In this study, we tackled the RNA-programmed synthesis of a 16mer peptide derived from the Bak-BH3 protein which inhibits the antiapoptotic protein Bcl-xL. The reaction involves the transfer of a thioester-linked donor peptide fragment from one PNA conjugate onto an acceptor peptide-PNA conjugate...
January 20, 2017: Chembiochem: a European Journal of Chemical Biology
keyword
keyword
102758
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"