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https://www.readbyqxmd.com/read/28088076/in%C3%A2-vivo-visualization-of-endogenous-mir-21-using-hyaluronic-acid-coated-graphene-oxide-for-targeted-cancer-therapy
#1
Do Won Hwang, Han Young Kim, Fangyuan Li, Ji Yong Park, Dohyun Kim, Jae Hyung Park, Hwa Seung Han, Jung Woo Byun, Yun-Sang Lee, Jae Min Jeong, Kookheon Char, Dong Soo Lee
Oncogene-targeted nucleic acid therapy has been spotlighted as a new paradigm for cancer therapeutics. However, in vivo delivery issues and uncertainty of therapeutic antisense drug reactions remain critical hurdles for a successful targeted cancer therapy. In this study, we developed a fluorescence-switchable theranostic nanoplatform using hyaluronic acid (HA)-conjugated graphene oxide (GO), which is capable of both sensing oncogenic miR-21 and inhibiting its tumorigenicity simultaneously. Cy3-labeled antisense miR-21 peptide nucleic acid (PNA) probes loaded onto HA-GO (HGP21) specifically targeted CD44-positive MBA-MB231 cells and showed fluorescence recovery by interacting with endogenous miR-21 in the cytoplasm of the MBA-MB231 cells...
December 31, 2016: Biomaterials
https://www.readbyqxmd.com/read/28087531/salivary-gluten-degradation-and-oral-microbial-profiles-in-health-and-celiac-disease
#2
Na Tian, Lina Faller, Daniel A Leffler, Ciaran P Kelly, Joshua Hansen, Jos A Bosch, Guoxian Wei, Bruce J Paster, Detlef Schuppan, Eva J Helmerhorst
: Celiac disease (CD) is a chronic immune-mediated enteropathy induced by dietary gluten in genetically predisposed individuals. Saliva harbors the second highest bacterial load of the GI tract after the colon. Moreover, saliva contains gluten-degrading enzymes produced by oral bacteria that may modify gluten processing in the intestine and susceptibility to celiac disease (CD). AIM: To investigate salivary enzymatic activities and oral microbial profiles in healthy subjects vs patients with classical and refractory CD...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28071057/programmed-transfer-of-sequence-information-into-molecularly-imprinted-polymer-mip-for-hexa-2-2-bithien-5-yl-dna-analog-formation-towards-single-nucleotide-polymorphism-snp-detection
#3
Katarzyna Bartold, Agnieszka Pietrzyk-Le, Tan-Phat Huynh, Zofia Iskierko, Marta I Sosnowska, Krzysztof Noworyta, Wojciech Lisowski, Francesco Maria Enrico Sannicolo, Silvia Cauteruccio, Emanuela Licandro, Francis D'Souza, Wlodzimierz Kutner
A new strategy of simple, inexpensive, rapid, and label-free single nucleotide polymorphism (SNP) detection using robust chemosensors with piezomicrogravimetric (PM), SPR, or capacitive impedimetry (CI) signal transduction is reported. Using these chemosensors, selective detection of a genetically relevant oligonucleotide under FIA conditions within 2 min is accomplished. An invulnerable to non-specific interaction molecularly imprinted polymer (MIP) with electrochemically synthesized probes of hexameric 2,2'-bithien-5-yl DNA analogs discriminating single purine-nucleobase mismatch at room temperature was used...
January 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28064147/experimental-and-theoretical-studies-on-stability-of-new-stabilizers-for-n-methyl-p-nitroaniline-derivative-in-cmdb-propellants
#4
Qiufan Tang, Xuezhong Fan, Jizhen Li, Fuqiang Bi, Xiaolong Fu, Lianjie Zhai
Although N-methyl-P-nitroaniline (MNA) was a quite effective stabilizer in composite modified double base (CMDB) propellants, it undergoes crystallization easily from nitroglycerin (NG) during storage. In order to improve its solubility in nitroglycerin (NG) and the stability in propellants, several new stabilizers including N-ethyl-p-nitroaniline (ENA), N-n-propyl-p-nitroaniline (n-PNA), N-i-propyl-p-nitroaniline (i-PNA), N-n-butyl-p-nitroaniline (n-BNA) and N-t-butyl-p-nitroaniline (t-BNA) were designed and synthesized to replace MNA by increasing the carbon chain length...
December 28, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28062294/assessment-of-biofilm-formation-in-pseudomonas-aeruginosa-by-antisense-maze-pna
#5
Hassan Valadbeigi, Nourkhoda Sadeghifard, Majid Baseri Salehi
The hallmark patogenicity in Pseudomonas aeruginosa (P. aeruginosa) is biofilm formation that is not easy to eradicate, because it has variety mechanisms for antibiotic resistance. In addition, toxin-antitoxin (TA) system may play role in biofilm formation. The current study aimed to evaluate the role of TA loci in biofilm formation. Therefore, 18 P. aeruginosa clinical isolates were collected and evaluated for specific biofilm and TA genes. The analysis by RT-qPCR demonstrated that expression of mazE antitoxin in biofilm formation was increase...
January 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28058638/functional-analysis-of-the-two-cyclophilin-isoforms-of-sinorhizobium-meliloti
#6
Eirini-Evangelia Thomloudi, Aggeliki Skagia, Anastasia Venieraki, Panagiotis Katinakis, Maria Dimou
The nitrogen fixing Sinorhizobium meliloti possesses two genes, ppiA and ppiB, encoding two cyclophilin isoforms which belong to the superfamily of peptidyl prolyl cis/trans isomerases (PPIase, EC: 5.2.1.8). Here, we functionally characterize the two proteins and we demonstrate that both recombinant cyclophilins are able to isomerise the Suc-AAPF-pNA synthetic peptide but neither of them displays chaperone function in the citrate synthase thermal aggregation assay. Furthermore, we observe that the expression of both enzymes increases the viability of E...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28039443/heterogeneity-and-frequency-of-braf-mutations-in-primary-melanoma-comparison-between-molecular-methods-and-immunohistochemistry
#7
William Bruno, Claudia Martinuzzi, Virginia Andreotti, Lorenza Pastorino, Francesco Spagnolo, Bruna Dalmasso, Francesco Cabiddu, Marina Gualco, Alberto Ballestrero, Giovanna Bianchi-Scarrà, Paola Queirolo, Federica Grillo, Luca Mastracci, Paola Ghiorzo
Finding the best technique to identify BRAF mutations with a high sensitivity and specificity is mandatory for accurate patient selection for target therapy. BRAF mutation frequency ranges from 40 to 60% depending on melanoma clinical characteristics and detection technique used. Intertumoral heterogeneity could lead to misinterpretation of BRAF mutational status; this is especially important if testing is performed on primary specimens, when metastatic lesions are unavailable. Aim of this study was to identify the best combination of methods for detecting BRAF mutations (among peptide nucleic acid - PNA-clamping real-time PCR, immunohistochemistry and capillary sequencing) and investigate BRAF mutation heterogeneity in a series of 100 primary melanomas and a subset of 25 matched metastatic samples...
22, 2016: Oncotarget
https://www.readbyqxmd.com/read/28032398/cultivation-and-characterisation-of-the-surface-markers-and-carbohydrate-profile-of-human-corneal-endothelial-cells
#8
Richárd Nagymihály, Zoltán Veréb, Réka Albert, Laura Sidney, Harminder Dua, Andrew Hopkinson, Goran Petrovski
BACKGROUND: To characterise human corneal endothelial cell (HCEnC) cultures generated by the peel-and-digest method based on their surface protein/carbohydrate expression pattern. METHODS: QPCR was used to compare expression of Vimentin, CD90, Cytokeratin-19, ZO-1, Claudin 14 in cultured HCEnC and cell line B4G12 versus stromal cells. Fluorescence-activated cell sorting (FACS) was used to assess surface protein distribution of cultured and uncultured HCEnC. Distribution of surface proteins/ carbohydrates was visualised by immunofluorescent and lectin staining...
December 29, 2016: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/28031530/a-novel-assay-to-detect-calreticulin-mutations-in-myeloproliferative-neoplasms
#9
Valentina Rosso, Jessica Petiti, Enrico Bracco, Roberto Pedrola, Francesca Carnuccio, Elisabetta Signorino, Sonia Carturan, Chiara Calabrese, Giada Bot-Sartor, Michela Ronconi, Anna Serra, Giuseppe Saglio, Francesco Frassoni, Daniela Cilloni
The myeloproliferative neoplasms are chronic myeloid cancers divided in Philadelphia positive (Ph+), chronic myeloid leukemia, or negative: polycythemia vera (PV) essential thrombocythemia (ET), and primary myelofibrosis (PMF). Most Ph negative cases have an activating JAK2 or MPL mutation. Recently, somatic mutations in the calreticulin gene (CALR) were detected in 56-88% of JAK2/MPL-negative patients affected by ET or PMF. The most frequent mutations in CARL gene are type-1 and 2. Currently, CALR mutations are evaluated by sanger sequencing...
December 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/28030624/pseudomonas-aeruginosa-microcolonies-in-coronary-thrombi-from-patients-with-st-segment-elevation-myocardial-infarction
#10
Gorm Mørk Hansen, Daniel Belstrøm, Martin Nilsson, Steffen Helqvist, Claus Henrik Nielsen, Palle Holmstrup, Tim Tolker-Nielsen, Michael Givskov, Peter Riis Hansen
Chronic infection is associated with an increased risk of atherothrombotic disease and direct bacterial infection of arteries has been suggested to contribute to the development of unstable atherosclerotic plaques. In this study, we examined coronary thrombi obtained in vivo from patients with ST-segment elevation myocardial infarction (STEMI) for the presence of bacterial DNA and bacteria. Aspirated coronary thrombi from 22 patients with STEMI were collected during primary percutaneous coronary intervention and arterial blood control samples were drawn from radial or femoral artery sheaths...
2016: PloS One
https://www.readbyqxmd.com/read/28029249/structure-and-function-of-aapeptides
#11
Olapeju Bolarinwa, Alekhya Nimmagadda, Ma Su, Jianfeng Cai
The intrinsic drawbacks encountered in bioactive peptides in chemical biology and biomedical sciences have diverted research efforts to the development of sequence-specific peptidomimetics which are capable of mimicking the structure and function of peptides and proteins. Modifications in the backbone and/or the side chain of peptides have been explored to develop biomimetic molecular probes or drug leads for biologically important targets. To expand the family of oligomeric peptidomimetics so as to facilitate their further application, we recently developed a new class of peptidomimetics - AApeptides based on chiral peptide nucleic acid (PNA) backbone...
December 28, 2016: Biochemistry
https://www.readbyqxmd.com/read/28010840/electrochemical-paper-based-peptide-nucleic-acid-biosensor-for-detecting-human-papillomavirus
#12
Prinjaporn Teengam, Weena Siangproh, Adisorn Tuantranont, Charles S Henry, Tirayut Vilaivan, Orawon Chailapakul
A novel paper-based electrochemical biosensor was developed using an anthraquinone-labeled pyrrolidinyl peptide nucleic acid (acpcPNA) probe (AQ-PNA) and graphene-polyaniline (G-PANI) modified electrode to detect human papillomavirus (HPV). An inkjet printing technique was employed to prepare the paper-based G-PANI-modified working electrode. The AQ-PNA probe baring a negatively charged amino acid at the N-terminus was immobilized onto the electrode surface through electrostatic attraction. Electrochemical impedance spectroscopy (EIS) was used to verify the AQ-PNA immobilization...
February 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28005284/multiplex-pna-fish-to-detect-cystic-fibrosis-polymicrobial-communities
#13
(no author information available yet)
No abstract text is available yet for this article.
February 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27997751/identifying-the-plant-associated-microbiome-across-aquatic-and-terrestrial-environments-the-effects-of-amplification-method-on-taxa-discovery
#14
Sara L Jackrel, Sarah M Owens, Jack A Gilbert, Catherine A Pfister
Plants in terrestrial and aquatic environments contain a diverse microbiome. Yet, the chloroplast and mitochondria organelles of the plant eukaryotic cell originate from free-living cyanobacteria and Rickettsiales. This represents a challenge for sequencing the plant microbiome with universal primers, as ~99% of 16S rRNA sequences may consist of chloroplast and mitochondrial sequences. Peptide nucleic acid clamps offer a potential solution by blocking amplification of host-associated sequences. We assessed the efficacy of chloroplast and mitochondria-blocking clamps against a range of microbial taxa from soil, freshwater, and marine environments...
December 20, 2016: Molecular Ecology Resources
https://www.readbyqxmd.com/read/27995649/prediction-of-serum-theophylline-concentrations-and-cyp1a2-activity-by-analyzing-urinary-metabolites-in-preterm-infants
#15
Jin A Sohn, Han-Suk Kim, Jaeseong Oh, Joo-Youn Cho, Kyung-Sang Yu, Juyoung Lee, Seung Han Shin, Jin A Lee, Chang Won Choi, Ee-Kyung Kim, Beyong Il Kim, Eun Ae Park
AIMS: The purpose of this study was to explore clinical markers reflecting developmental changes in drug clearance by preterm infants. METHODS: Preterm infants administered aminophylline or theophylline to treat apnoea of prematurity were enrolled in this study. Trough and one of 2 h, 4 h or 6 h post-dose blood samples and urine samples were collected during steady state, to determine the concentrations of theophylline and its targeted metabolites. CYP1A2*1C and CYP1A2*1 F genotypes were analyzed...
December 20, 2016: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/27986898/antimicrobial-activity-of-antisense-peptide-peptide-nucleic-acid-conjugates-against-non-typeable-haemophilus-influenzae-in-planktonic-and-biofilm-forms
#16
Taketo Otsuka, Aimee L Brauer, Charmaine Kirkham, Erin K Sully, Melinda M Pettigrew, Yong Kong, Bruce L Geller, Timothy F Murphy
BACKGROUND: Antisense peptide nucleic acids (PNAs) are synthetic polymers that mimic DNA/RNA and inhibit bacterial gene expression in a sequence-specific manner. METHODS: To assess activity against non-typeable Haemophilus influenzae (NTHi), we designed six PNA-peptides that target acpP, encoding an acyl carrier protein. MICs and minimum biofilm eradication concentrations (MBECs) were determined. Resistant strains were selected by serial passages on media with a sub-MIC concentration of acpP-PNA...
January 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27983655/fabrication-of-a-horizontal-and-a-vertical-large-surface-area-nanogap-electrochemical-sensor
#17
Jules L Hammond, Mark C Rosamond, Siva Sivaraya, Frank Marken, Pedro Estrela
Nanogap sensors have a wide range of applications as they can provide accurate direct detection of biomolecules through impedimetric or amperometric signals. Signal response from nanogap sensors is dependent on both the electrode spacing and surface area. However, creating large surface area nanogap sensors presents several challenges during fabrication. We show two different approaches to achieve both horizontal and vertical coplanar nanogap geometries. In the first method we use electron-beam lithography (EBL) to pattern an 11 mm long serpentine nanogap (215 nm) between two electrodes...
December 14, 2016: Sensors
https://www.readbyqxmd.com/read/27981833/isoxazolidine-a-privileged-scaffold-for-organic-and-medicinal-chemistry
#18
Mathéo Berthet, Thomas Cheviet, Gilles Dujardin, Isabelle Parrot, Jean Martinez
The isoxazolidine ring represents one of the privileged structures in medicinal chemistry, and there have been an increasing number of studies on isoxazolidine and isoxazolidine-containing compounds. Optimization of the 1,3-dipolar cycloaddition (1,3-DC), original methods including electrophilic or palladium-mediated cyclization of unsaturated hydroxylamine, has been developed to obtain isoxazolidines. Novel reactions involving the isoxazolidine ring have been highlighted to accomplish total synthesis or to obtain bioactive compounds, one of the most significant examples being probably the thermic ring contraction applied to the total synthesis of (±)-Gelsemoxonine...
December 28, 2016: Chemical Reviews
https://www.readbyqxmd.com/read/27973633/potentiometric-sensing-of-nucleic-acids-using-chemically-modified-nanopores
#19
István Makra, Alexandra Brajnovits, Gyula Jágerszki, Péter Fürjes, Róbert E Gyurcsányi
Unlike the overwhelming majority of nanopore sensors that are based on the measurement of a transpore ionic current, here we introduce a potentiometric sensing scheme and demonstrate its application for the selective detection of nucleic acids. The sensing concept uses the charge inversion that occurs in the sensing zone of a nanopore upon binding of negatively charged microRNA strands to positively charged peptide-nucleic acid (PNA) modified nanopores. The initial anionic permselectivity of PNA-modified nanopores is thus gradually changed to cationic permselectivity, which can be detected simply by measuring the nanoporous membrane potential...
December 14, 2016: Nanoscale
https://www.readbyqxmd.com/read/27965271/different-mechanisms-of-na-uptake-and-ammonia-excretion-by-the-gill-and-yolk-sac-epithelium-of-early-life-stage-rainbow-trout
#20
Alex M Zimmer, Jonathan M Wilson, Patricia A Wright, Junya Hiroi, Chris M Wood
In rainbow trout, the dominant site of Na(+) uptake (J(Na)in) and ammonia excretion (Jamm) shifts from the skin to the gills over development. Post-hatch (PH; 7 days post-hatch) larvae utilize the yolk sac skin for physiological exchange, whereas by complete yolk sac absorption (CYA; 30 days post-hatch), the gill is the dominant site. At the gills, J(Na)in and Jamm occur via loose Na(+)/NH4(+) exchange, but this exchange has not been examined in the skin of larval trout. Based on previous work, we hypothesized that, contrary to the gill model, J(Na)in by the yolk sac skin of PH trout occurs independently of Jamm Following a 12-h exposure to high environmental ammonia (HEA; 0...
December 13, 2016: Journal of Experimental Biology
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