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https://www.readbyqxmd.com/read/28811020/peanut-agglutinin-and-%C3%AE-cyclodextrin-functionalized-polymer-monolith-microextraction-of-igg-galactosylation-coupled-with-online-ms-detection
#1
Haijiao Zheng, Tenggao Zhu, Xiqian Li, Jiutong Ma, Qiong Jia
A facile online method coupling polymer monolithic microextraction (PMME) with mass spectrometry (MS) was developed for the detection of Immunoglobulin G (IgG) galactosylation glycopeptides. A peanut agglutinin-β-cyclodextrin (PNA-β-CD) functionalized poly(hydroxyethyl methylacrylate-ethyleneglycol dimethacrylate) monolith was designed via a click reaction. Thanking to the specificity of PNA-β-CD for the targets, the material exhibited enhanced enrichment selectivity and extraction efficiency for IgG galactosylation glycopeptides...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28810153/evaluation-of-affibody-molecule-based-pna-mediated-radionuclide-pretargeting-development-of-an-optimized-conjugation-protocol-and-177-lu-labeling
#2
Mohamed Altai, Kristina Westerlund, Justin Velletta, Bogdan Mitran, Hadis Honarvar, Amelie Eriksson Karlström
INTRODUCTION: We have previously developed a pretargeting approach for affibody-mediated cancer therapy based on PNA-PNA hybridization. In this article we have further developed this approach by optimizing the production of the primary agent, ZHER2:342-SR-HP1, and labeling the secondary agent, HP2, with the therapeutic radionuclide (177)Lu. We also studied the biodistribution profile of (177)Lu-HP2 in mice, and evaluated pretargeting with (177)Lu-HP2 in vitro and in vivo. METHODS: The biodistribution profile of (177)Lu-HP2 was evaluated in NMRI mice and compared to the previously studied (111)In-HP2...
July 12, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/28800650/peptide-nucleic-acid-fluorescence-in-situ-hybridization-for-detection-of-staphylococci-from-endophthalmitis-isolates-a-proof-of-concept-study
#3
Nimesh Patel, Darlene Miller, Nidhi Relhan, Harry W Flynn
Purpose: Rapid identification of pathogens causing endophthalmitis may improve treatment outcomes through early administration of species-specific medication. The current study reports a new molecular application of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with Staphylococcus-specific molecular PNA probes for the potential rapid detection of common pathogens causing endophthalmitis. Methods: An experimental study was designed to evaluate the proof of concept at the microbiology laboratory of the Bascom Palmer Eye Institute...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28795699/photoexcitation-dynamics-of-p-nitroaniline-and-n-n-dimethyl-p-nitroaniline-in-1-alkyl-3-methylimidazolium-cation-based-ionic-liquids-with-different-alkyl-chain-lengths
#4
Y Kimura, S Ibaraki, R Hirano, Y Sugita, Y Yasaka, M Ueno
Photoexcitation dynamics of p-nitroaniline (pNA) and N,N-dimethyl-p-nitroaniline (DMpNA) in 1-alkyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ([Cnmim][NTf2]) with different alkyl chain lengths (from C2 to C12) was investigated using transient absorption spectroscopy. The internal conversion rate from the excited state to the ground state was estimated from bleach recovery around the ground state absorption centre, and the successive vibrational cooling rate in the ground state was estimated from the decay of the hot band observed at the red-edge of ground state absorption...
August 10, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28794451/vitamin-b12-as-a-carrier-of-peptide-nucleic-acid-pna-into-bacterial-cells
#5
Marcin Równicki, Monika Wojciechowska, Aleksandra J Wierzba, Jakub Czarnecki, Dariusz Bartosik, Dorota Gryko, Joanna Trylska
Short modified oligonucleotides targeted at bacterial DNA or RNA could serve as antibacterial agents provided that they are efficiently taken up by bacterial cells. However, the uptake of such oligonucleotides is hindered by the bacterial cell wall. To overcome this problem, oligomers have been attached to cell-penetrating peptides, but the efficiency of delivery remains poor. Thus, we have investigated the ability of vitamin B12 to transport peptide nucleic acid (PNA) oligomers into cells of Escherichia coli and Salmonella Typhimurium...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28772161/catalytic-amidolysis-of-amino-acid-p-nitroanilides-using-transition-state-analogue-imprinted-artificial-enzymes-cooperative-effect-of-pyridine-moiety
#6
Mathew Divya, Thomas Benny, Philip Christy, E P Aparna, K S Devaky
Enzyme-like polymer catalysts with the imprints of phosphonate transition state analogue (TSA) lined along with imidazole and pyridine moieties were synthesized using methacryloyl-l-histidine and 4-vinylpyridine as the functional monomers and phenyl-1-(N-benzyloxycarbonylamino)-2-(phenyl)ethyl phosphonate - the TSA of hydrolytic reaction as the template for the amidolysis of N-benzyloxycarbonyl-l-phenylalanine p-nitroanilide (Z-l-Phe-PNA). Polymers containing different functional groups can act together to provide catalytic activity and selectivity superior to what can be obtained from monofunctional analogues...
July 25, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28766647/facile-functionalization-of-peptide-nucleic-acids-pnas-for-antisense-and-single-nucleotide-polymorphism-detection
#7
Digvijay Gahtory, Merita Murtola, Maarten M J Smulders, Tom Wennekes, Han Zuilhof, Roger Strömberg, Bauke Albada
In this report, we show how a convenient on-resin copper-click functionalization of azido-functionalized peptide nucleic acids (PNAs) allows various PNA-based detection strategies. Firstly, a thiazole orange (TO) clicked PNA probe facilitates a binary readout when combined with F/Q labeled DNA, giving increased sensitivity for antisense detection. Secondly, our TO-PNA conjugate also allows single nucleotide polymorphism detection. Since antisense detection is also possible in the absence of the TO label, our sensing platform based on azido-d-ornithine containing PNA even allows for additional and more advanced functionalization and sensing strategies...
August 16, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28764969/peptide-nucleic-acids-advanced-tools-for-biomedical-applications
#8
REVIEW
Anjali Gupta, Anuradha Mishra, Nidhi Puri
Peptide Nucleic Acids (PNAs) are the DNA/RNA analogues in which sugar-phosphate backbone is replaced by N-2-aminoethylglycine repeating units. PNA contains neutral backbone hence due to the absence of electrostatic repulsion, its hybridization shows remarkable stability towards complementary oligonucleotides. PNAs are highly resistant to cleavage by chemicals and enzymes due to the substrate specific nature of enzymes and therefore not degraded inside the cells. PNAs are emerging as new tools in the market due to their applications in antisense and antigene therapies by inhibiting translation and transcription respectively...
July 29, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28760897/tools-for-the-individualized-therapy-of-teicoplanin-for-neonates-and-children
#9
V Ramos-Martín, M N Neely, K Padmore, M Peak, M W Beresford, M A Turner, S Paulus, J López-Herce, W W Hope
The aim of the study was to develop a population PK model for teicoplanin across childhood age ranges to be used as Bayesian prior information in the constructed software for individualized therapy. We developed a non-parametric population model fitted to PK data from neonates, infants and older children. We then implemented it in the BestDose multiple-model Bayesian adaptive control algorithm to show its clinical utility. It was used to predict the required dosages to achieve teicoplanin optimal pre-dose targets (15 mg/L) from day 3 of therapy...
July 31, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28756875/glycopattern-analysis-of-acidic-secretion-in-the-intestine-of-the-red-eared-slender-turtle-trachemys-scripta-elegans-testudines-emydidae
#10
Giovanni Scillitani, Donatella Mentino, Maria Mastrodonato
The secretion of the goblet cells in the intestine of Trachemys scripta elegans was studied in situ by histochemical methods to analyze the diversity of sugar chains, with particular regard to the acidic glycans. Conventional histochemical stains (Periodic acid-Schiff, Alcian Blue pH 2.5, High Iron Diamine) and binding with ten FITC-labelled lectins combined with chemical and enzymatic pre-treatments were used to characterize the oligosaccharidic chains. The intestine can be divided into three regions, i.e...
July 23, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28746942/screening-for-retinopathy-of-prematurity-insight-into-optimizing-screening
#11
Charlotte Pallot, Agnès Soudry Faure, Camille Charvy, Ninon Ternoy, Claire Langlet Muteau, Niyazi Acar, Alain M Bron, Claude Speeg Schatz, Catherine Creuzot-Garcher
PURPOSE: To determine the factors influencing the time from preterm birth and retinopathy of prematurity (ROP) detection to optimize the timing of the initial screening. METHODS: This multicenter retrospective study enrolled preterm infants born before 32 weeks of gestational age (GA) and/or weighing less than 1,500 g between January 1, 2011, and December 31, 2015. ROP screening was performed using fundus photography with a wide-field camera. Population and follow-up characteristics were recorded...
July 27, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/28730445/polycationic-probe-guided-nanopore-single-molecule-counter-for-selective-mirna-detection
#12
Kai Tian, Ruicheng Shi, Amy Gu, Michael Pennella, Li-Qun Gu
MicroRNAs (miRNAs) are a class of noncoding RNAs that are being explored as a new type of disease biomarkers. The nanopore single-molecule sensor offers a potential noninvasive tool to detect miRNAs for diagnostics and prognosis applications. However, one of the challenges that limits its clinical applications is the presence of a large variety of nontarget nucleic acids in the biofluid extracts. Upon interacting with the nanopore, nontarget nucleic acids produce "contaminative" nanopore signals that interfere with target miRNA discrimination, thus severely lowering the accuracy in target miRNA detection...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28728975/continuous-nitrogen-removal-by-a-single-stage-reactor-packed-with-ring-laced-string-medium
#13
Masahiro Tatara, Shu Ishikawa, Yoshiyuki Ueno
The efficiency of nitrogen removal by a partial-nitritation/anammox (PNA) reaction was investigated using a packed-bed reactor in which ring-laced strings were used as the supporting medium. A stable population of PNA microorganisms was established from typical activated sludge, after less than two months of acclimation in the packed-bed reactor, by applying a high nitrogen-loading rate (NLR: 0.53 kg/m(3)/d) and short hydraulic retention time (HRT: 1.8 h). The stability of reactor performance was confirmed in industrial wastewater (IW), demonstrating a nitrogen removal efficiency (NRE) of greater than 77% during 260 days of continuous operation, between 0...
July 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28709076/coupling-autotrophic-denitrification-with-partial-nitritation-anammox-pna-for-efficient-total-inorganic-nitrogen-removal
#14
Sunayna Dasgupta, Sha Wu, Ramesh Goel
The performance of and the microbial ecology in an integrated lab scale set up comprising of a PN/A bioreactor and an elemental sulfur-supported packed bed autotrophic denitrification (ESSAD) are reported. The PN/A reactor exhibited an average removal rate of 0.56±0.103kgNm(-3)d(-1), whereas the ESSAD reactor removed an average of 0.0018kg NO3(-)-Nm(-3)d(-1). The combined average removal rate was 0.6kgNm(-3)d(-1), yielding an overall total inorganic nitrogen efficiency of 97%. Based on 16S rRNA gene clone libraries from the ESSAD reactor, the extracted Operational Taxonomic Units (OTUs) formed a clade with Thiobacillus denitrificans sp...
June 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28704609/hydrophilic-and-cell-penetrable-pyrrolidinyl-peptide-nucleic-acid-via-post-synthetic-modification-with-hydrophilic-side-chains
#15
Haruthai Pansuwan, Boonsong Ditmangklo, Chotima Vilaivan, Banphot Jiangchareon, Porntip Pan-In, Supason Wanichwecharungruang, Tanapat Palaga, Thanesuan Nuanyai, Chaturong Suparpprom, Tirayut Vilaivan
Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose-phosphate was replaced by a peptide-like backbone. The absence of negative charge in the PNA backbone leads to several unique behaviors including a stronger binding and salt independency of the PNA-DNA duplex stability. However, PNA possesses poor aqueous solubility and cannot directly penetrate cell membranes. These are major obstacles that limit in vivo applications of PNA. In previous strategies, the PNA can be conjugated to macromolecular carriers or modified with positively charged side chains such as guanidinium groups to improve the aqueous solubility and cell permeability...
August 11, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28699197/sperm-telomere-length-in-motile-sperm-selection-techniques-a-qfish-approach
#16
R Lafuente, E Bosch-Rue, J Ribas-Maynou, J Alvarez, C Brassesco, M J Amengual, J Benet, A Garcia-Peiró, M Brassesco
Several studies have associated telomere shortening with alterations in reproductive function. The objective of the present study was to determine telomere length (TL) in spermatozoa selected by either density-gradient centrifugation (DGC) or swim-up. The analysis of TL was performed using quantitative fluorescent in situ hybridisation (qFISH) using PNA probes in combination with a chromatin decompaction protocol in sperm cells. Results of TL were 24.64 ± 5.00 Kb and 24.95 ± 4.60 Kb before and after DGC, respectively, and 19...
July 11, 2017: Andrologia
https://www.readbyqxmd.com/read/28698463/peptide-nucleic-acids-as-mirna-target-protectors-for-the-treatment-of-cystic-fibrosis
#17
Federica Zarrilli, Felice Amato, Carmine Marco Morgillo, Brunella Pinto, Giuliano Santarpia, Nicola Borbone, Stefano D'Errico, Bruno Catalanotti, Gennaro Piccialli, Giuseppe Castaldo, Giorgia Oliviero
Cystic Fibrosis (CF) is one of the most common life shortening conditions in Caucasians. CF is caused by mutations in the CF Transmembrane Conductance Regulator (CFTR) gene which result in reduced or altered CFTR functionality. Several microRNAs (miRNAs) downregulate the expression of CFTR, thus causing or exacerbating the symptoms of CF. In this context, the design of anti-miRNA agents represents a valid functional tool, but its translation to the clinic might lead to unpredictable side effects because of the interference with the expression of other genes regulated by the same miRNAs...
July 8, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28696677/monolithic-peptide-nucleic-acid-hybrid-functioning-as-an-artificial-microperoxidase
#18
Koji Nakano, Junichi Tanabe, Ryoich Ishimatsu, Toshihiko Imato
A new peptide nucleic acid (PNA) with an installed peroxidase function has been developed. Fmoc solid phase peptide synthesis prepared a PNA hybrid (VQKCAQCHTVE-(C2H4O)2CH2-[PNA(T)]6-G) that renders the microperoxidase backbone, followed by reconstitution with hemin. The resulting holocompound catalyzed the oxidation of 3,3',5,5'-tetramthylbenzidine by H2O2 to 50% that of natural microperoxidase-11, whereas the apo-form and hemin gave no responses. The peroxidase domain was found to be active toward direct electrochemistry and the PNA hybrid served for gene sensor; in the presence of the target DNA (5'-CATGTATAAAAAA-3'), an electrode-attached DNA probe (5'-TsTsTsTsTsTCTCATACATG-3') showed the ferric-to-ferrous quasi-reversible wave (-276 mV vs Ag/AgCl) through sandwich hybridization...
July 25, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28695635/taurine-does-not-improve-the-quality-of-short-term-stored-rabbit-spermatozoa-in-vitro
#19
D Paál, F Strejček, E Tvrdá, J Vašíček, A Baláži, P Chrenek, P Massányi
This study examines the impact of taurine on the viability, morphology and acrosome integrity of rabbit spermatozoa in vitro. Semen samples, obtained from four to five sexually mature and healthy New Zealand White rabbits, were pooled in heterospermic semen sample. This was divided and treated with taurine in a concentration of 0 (control), 1.5, 7, 12.5, 50 mM to a final concentration of 10(8)  spermatozoa/ml. The samples were then incubated at 37°C for 4 hr. A combination of fluorescent probes SYBR-14/propidium iodide/PNA-Alexa Fluor 647 was used to assess spermatozoa viability and acrosome integrity on a flow cytometer...
July 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28690651/mutations-of-the-epidermal-growth-factor-receptor-gene-in-triple-negative-breast-cancer
#20
Aeri Kim, Min Hye Jang, Soo Jung Lee, Young Kyung Bae
PURPOSE: Epidermal growth factor receptor (EGFR) is considered a potential therapeutic target for anti-EGFR therapy in triple-negative breast cancer (TNBC). However, the frequency of EGFR gene mutation in TNBC is low and varies with ethnicity. This study aimed to investigate the incidence of EGFR gene mutation in TNBC. METHODS: EGFR protein expression was evaluated by immunohistochemistry in tissue microarrays of 493 TNBC cases using four different primary antibodies, which included mutation-specific antibodies...
June 2017: Journal of Breast Cancer
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