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https://www.readbyqxmd.com/read/28431414/intratracheal-instillation-of-neutrophils-rescues-bacterial-overgrowth-initiated-by-trauma-damage-associated-molecular-patterns
#1
Kiyoshi Itagaki, Ingred Riça, Jing Zhang, Dave Gallo, Melissa DePrato, Leo E Otterbein, Carl J Hauser
BACKGROUND: Nosocomial pneumonias are common in trauma patients and so interventions to prevent and treat nosocomial pneumonia may improve outcomes. Our prior work strongly suggests that tissue injury predisposes to infections like nosocomial pneumonia because mitochondrial debris originating from injured cells contains damage-associated molecular patterns that can reduce neutrophil (PMN) migration into the airway and diminish PMN function in response to bacterial inoculation of the airway...
May 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28429810/cisplatin-directed-coordination-crosslinking-nanogels-with-thermo-ph-sensitive-triblock-polymers-improvement-on-chemotherapic-efficacy-via-sustained-release-and-drug-retention
#2
Hao Zhao, Jiabao Xu, Jiangshan Wan, Shinan Geng, Han Li, Xiaole Peng, Qianwen Fu, Ming He, Yanbing Zhao, Xiangliang Yang
To realize the sustained release and long-term intratumoural retention of water-soluble cisplatin, thermo/pH-sensitive cisplatin-directed coordination-crosslinking nanogels (Pt-PNA) were developed via the coordination bonds of Pt-carboxyl groups. As the coordination ratio (CR) of the Pt-carboxyl bonds increased from 5% to 35%, the sizes of the Pt-PNA nanogels decreased from 999 nm to 167 nm, and their zeta potentials increased from -35 mV to -13 mV. Only through a simple mixing of cisplatin and PNAs, the entrapment efficiencies (EEs) of the Pt-PNA nanogels reached near 100% (>90%), and the drug-loading amounts (DLs) of cisplatin could achieve up to 25...
April 21, 2017: Nanoscale
https://www.readbyqxmd.com/read/28423373/distribution-of-interstitial-telomeric-sequences-in-primates-and-the-pygmy-tree-shrew-scandentia
#3
Sofia Mazzoleni, Odessa Schillaci, Luca Sineo, Francesca Dumas
It has been hypothesized that interstitial telomeric sequences (ITSs), i.e., repeated telomeric DNA sequences found at intrachromosomal sites in many vertebrates, could be correlated to chromosomal rearrangements and plasticity. To test this hypothesis, we hybridized a telomeric PNA probe through FISH on representative species of 2 primate infraorders, Strepsirrhini (Lemur catta, Otolemur garnettii, Nycticebus coucang) and Catarrhini (Erythrocebus patas, Cercopithecus petaurista, Chlorocebus aethiops, Colobus guereza), as well as on 1 species of the order Scandentia, Tupaia minor, used as an outgroup for primates in phylogenetic reconstructions...
April 20, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/28420874/therapeutic-targeting-using-tumor-specific-peptides-inhibits-long-non-coding-rna-hotair-activity-in-ovarian-and-breast-cancer
#4
Ali R Özeş, Yinu Wang, Xingyue Zong, Fang Fang, Jay Pilrose, Kenneth P Nephew
Long non-coding RNAs (lncRNAs) play key roles in human diseases, including cancer. Functional studies of the lncRNA HOTAIR (HOX transcript antisense RNA) provide compelling evidence for therapeutic targeting of HOTAIR in cancer, but targeting lncRNAs in vivo has proven to be difficult. In the current study, we describe a peptide nucleic acids (PNA)-based approach to block the ability of HOTAIR to interact with EZH2 and subsequently inhibit HOTAIR-EZH2 activity and resensitize resistant ovarian tumors to platinum...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418159/toxicity-of-sediment-associated-substituted-phenylamine-antioxidants-on-the-early-life-stages-of-pimephales-promelas-and-a-characterization-of-effects-on-freshwater-organisms
#5
Ryan S Prosser, Joanne L Parrott, Melissa Galicia, Kallie Shires, Cheryl Sullivan, John Toito, Adrienne J Bartlett, Danielle Milani, Patty L Gillis, Vimal K Balakrishnan
Substituted phenylamine antioxidants (SPAs) are high production volume chemicals that are incorporated into a variety of commercial products (e.g., polymers, dyes, lubricants). There are little data on chronic toxicity of SPAs to fish and no data on the toxicity of SPAs to the early-life stages of fish. The physicochemical properties of SPAs would suggest that if they were to enter an aquatic ecosystem they would partition into sediment. Therefore, the current study focused on investigating the chronic effect of sediment-associated SPAs to the early-life stages of the fathead minnow (Pimephales promelas)...
April 18, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28414202/recent-advances-in-peptide-nucleic-acid-for-cancer-bionanotechnology
#6
REVIEW
Jun-Chen Wu, Qing-Chun Meng, Hong-Mei Ren, Hong-Tao Wang, Jie Wu, Qi Wang
Peptide nucleic acid (PNA) is an oligomer, in which the phosphate backbone has been replaced by a pseudopeptide backbone that is meant to mimic DNA. Peptide nucleic acids are of the utmost importance in the biomedical field because of their ability to hybridize with neutral nucleic acids and their special chemical and biological properties. In recent years, PNAs have emerged in nanobiotechnology for cancer diagnosis and therapy due to their high affinity and sequence selectivity toward corresponding DNA and RNA...
April 17, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28407432/diagnosis-of-biofilm-infections-in-cystic-fibrosis-patients
#7
REVIEW
Niels Høiby, Thomas Bjarnsholt, Claus Moser, Peter Østrup Jensen, Mette Kolpen, Tavs Qvist, Kasper Aanaes, Tanja Pressler, Marianne Skov, Oana Ciofu
Chronic Pseudomonas aeruginosa biofilm lung infection in cystic fibrosis patients is the best described biofilm infection in medicine. The initial focus can be the paranasal sinuses and then follows repeated colonization and infection of the lungs by aspiration. The matrix of the biofilms is dominated by alginate and the pathogenesis of tissue damage is immune complex-mediated chronic inflammation dominated by polymorphonuclear leukocytes and their products (DNA, oxygen radicals and proteases). The P. aeruginosa biofilm infection can be diagnosed by microscopy of lung tissue, sputum and mucus from the paranasal sinuses, where aggregates of the bacteria are found surrounded by the abundant alginate matrix...
April 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28405680/optimal-method-for-quantitative-detection-of-plasma-egfr-t790m-mutation-using-droplet-digital-pcr-system
#8
Ken Suzawa, Hiromasa Yamamoto, Kadoaki Ohashi, Shinsuke Hashida, Shuta Tomida, Toshio Kubo, Yuho Maki, Junichi Soh, Kazunori Tsukuda, Katsuyuki Kiura, Shinichiro Miyoshi, Shinichi Toyooka
Though patients with EGFR mutations are initially responsive to EGFR-tyrosine kinase inhibitors (TKIs), most tumors ultimately acquire resistance to EGFR-TKIs. The most frequently reported mechanism is EGFR T790M mutation. In this study, using a droplet digital PCR (ddPCR) system, we assessed optimal conditions for a mutation detection assay for EGFR T790M obtained from circulating cell-free DNA (cfDNA) in plasma. The advantages of locked nucleic acids (LNA) probe, short amplicon size, and blocking oligo using peptide nucleic acids (PNA) were assessed using control DNAs from cell lines to improve the sensitivity of mutation detection...
May 2017: Oncology Reports
https://www.readbyqxmd.com/read/28396015/helix-69-of-e-coli-23s-ribosomal-rna-as-a-peptide-nucleic-acid-target
#9
Marta Kulik, Agnieszka Markowska-Zagrajek, Monika Wojciechowska, Renata Grzela, Tomasz Wituła, Joanna Trylska
A fragment of 23S ribosomal RNA (nucleotides 1906-1924 in E. coli), termed Helix 69, forms a hairpin that is essential for ribosome function. Helix 69 forms a conformationally flexible inter-subunit connection with helix 44 of 16S ribosomal RNA, and the nucleotide A1913 of Helix 69 influences decoding accuracy. Nucleotides U1911 and U1917 are post-transcriptionally modified with pseudouridines () and U1915 with 3-methyl-. We investigated Helix 69 as a target for a complementary synthetic oligonucleotide - peptide nucleic acid (PNA)...
April 7, 2017: Biochimie
https://www.readbyqxmd.com/read/28394582/multiplex-paper-based-colorimetric-dna-sensor-using-pyrrolidinyl-peptide-nucleic-acid-induced-agnps-aggregation-for-detecting-mers-cov-mtb-and-hpv-oligonucleotides
#10
Prinjaporn Tee-Ngam, Weena Siangproh, Adisorn Tuantranont, Tirayut Vilaivan, Orawon Chailapakul, Charles S Henry
The development of simple fluorescent and colorimetric assays that enable point-of-care DNA and RNA detection has been a topic of significant research because of the utility of such assays in resource limited settings. The most common motifs utilize hybridization to a complementary detection strand coupled with a sensitive reporter molecule. Here, apaper-based colorimetric assay for DNA detection based on pyrrolidinyl peptide nucleic acid (acpcPNA)-induced nanoparticle aggregationis reported as an alternative to traditional colorimetric approaches...
April 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28389017/corrigendum-to-pna-combo-fish-from-combinatorial-probe-design-in-silico-to-vitality-compatible-specific-labelling-of-gene-targets-in-cell-nuclei-exp-cell-res-345-2016-51-59
#11
Patrick Müller, Jens Rößler, Jutta Schwarz-Finsterle, Eberhard Schmitt, Michael Hausmann
No abstract text is available yet for this article.
April 4, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28383031/serum-glycopatterns-as-novel-potential-biomarkers-for-diagnosis-of-acute-on-chronic-hepatitis-b-liver-failure
#12
Yaogang Zhong, Yonghong Guo, Xiawei Liu, Jiaxu Zhang, Tianran Ma, Jian Shu, Jiajun Yang, Jing Zhang, Zhansheng Jia, Zheng Li
Acute-on-chronic hepatitis B liver failure (ACHBLF) is an increasingly recognized distinct disease entity encompassing an acute deterioration of liver function in patients with cirrhosis, so little is known about the alterations of protein glycopatterns in serum with its development. We aimed to identify the alterations of serum glycopatterns in ACHBLF and probe the possibility of them as novel potential biomarkers for diagnosis of ACHBLF. As a result, there were 18 lectins (e.g., WFA, GSL-II, and PNA) to give significantly alterations of serum glycopatterns in ACHBLF compared with healthy controls (HC) (all p ≤ 0...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28370485/19f-nmr-spectroscopic-analysis-of-the-binding-modes-in-triple-helical-pna-microrna-complexes
#13
Pasi Markus Virta, Ville Tähtinen, Lotta Granqvist, Merita Murtola, Roger Strömberg
Triplex-forming peptide nucleic acids (TFPNAs) were targeted to double helical regions of 19F-labelled RNA hairpin models (a UA-rich duplex with a hexaethylene glycol (heg) loop and a miR-215 model). In addition to the conventional UV- and CD-based detection, the binding was monitored by 19F NMR spectroscopy. Detailed information considering the stoichiometry and transition between the triple helical PNA/RNA- and (PNA)2/RNA-binding modes could be obtained. γ-(R)-hydroxymethyl-modified (thymine-1-yl- and 2-aminopyridin-3-yl-acetyl derivatives) TFPNAs were additionally synthesized, targeted to the same RNA models and the effect of the γ-(R)-hydroxymethyl group on the binding was studied...
March 28, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28365456/mir-21-promotes-dengue-virus-serotype-2-replication-in-hepg2-cells
#14
Sitthichai Kanokudom, Tirayut Vilaivan, Nitwara Wikan, Chutima Thepparit, Duncan R Smith, Wanchai Assavalapsakul
Infection with the mosquito transmitted dengue virus (DENV) remains a significant worldwide public health problem. While the majority of infections are asymptomatic, infection can result in a range of symptoms. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through repression or degradation of mRNAs. To understand the contribution of miRNAs to DENV 2 replication, we screened a number of candidate miRNAs for variations in expression levels during DENV 2 infection of HepG2 (liver) cells...
March 30, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28363502/bovine-sperm-separation-by-swim-up-and-density-gradients-percoll-and-bovipure-effect-on-sperm-quality-function-and-gene-expression
#15
María Elena Arias, Katherine Andara, Evelyn Briones, Ricardo Felmer
This study assesses the effect of bovine sperm (obtained from three bulls) separation using density gradients (Percoll and BoviPure) and Swim-up on sperm function and gene expression. Sperm evaluations included the plasma membrane integrity (SYBR14/PI), acrosomal integrity (PNA-FITC/PI), oxidative stress (ROS; CH2FDDA), DNA fragmentation (TUNEL assay) and mitochondrial membrane potential (ΔYm; TMRM) using flow cytometry. Sperm motility was evaluated by computer-assisted sperm analysis (CASA) and gene expression using RT-qPCR...
March 28, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28362077/lactosylated-glycogen-nanoparticles-for-targeting-prostate-cancer-cells
#16
Quinn Alexander Besford, Marcin Wojnilowicz, Tomoya Suma, Nadja Bertleff-Zieschang, Frank Caruso, Francesca Cavalieri
Glyconanoparticles that exhibit multivalent binding to lectins are highly desirable for molecular recognition and therapeutic applications, for example. Herein, we explore the use of glycogen nanoparticles as a biosourced glyco-scaffold for engineering multivalent glyconanoparticles. Glycogen nanoparticles, a naturally occurring highly-branched polymer of glucose, was functionalized with lactose, achieved through copper(I)-catalyzed alkyne-azide cycloaddition chemistry, for targeted interaction with lectins ex situ and on prostate cancer cells...
March 31, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28355582/synthesis-of-%C3%AE-galactosylamides-as-ligands-of-the-peanut-lectin-insights-into-the-recognition-process
#17
María Emilia Cano, Oscar Varela, María Isabel García-Moreno, José Manuel García Fernández, José Kovensky, María Laura Uhrig
The synthesis of mono and divalent β-galactosylamides linked to a hydroxylated chain having a C2 symmetry axis derived from l-tartaric anhydride is reported. Reference compounds devoid of hydroxyl groups in the linker were also prepared from β-galactosylamine and succinic anhydride. After functionalization with an alkynyl residue, the resulting building blocks were grafted onto different azide-equipped scaffolds through the copper catalyzed azide-alkyne cycloaddition. Thus, a family of structurally related mono and divalent β-N-galactopyranosylamides was obtained and fully characterized...
March 23, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28345902/hyaluronate-peanut-agglutinin-conjugates-for-target-specific-bioimaging-of-colon-cancer
#18
Songeun Beack, Minsoo Cho, Young-Eun Kim, G-One Ahn, Sei Kwang Hahn
Colon cancer is one of the most common death-related cancers in the world. For treating colon cancer, it is crucial to detect and remove malignant lesions early. Here, we developed hyaluronate (HA)-peanut agglutinin (PNA) conjugates for the bioimaging of colon cancer. The HA-PNA conjugates were successfully synthesized by the coupling reaction between aldehyde-modified HA and the N-terminal amine group of PNA. For diagnostic imaging, rhodamine B (RhoB) was chemically conjugated onto PNA in HA-PNA conjugates...
April 5, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28340704/sub-attomolar-electrochemical-measurement-of-dna-hybridization-based-on-the-detection-of-high-coverage-biobarcode-latex-labels-at-pna-modified-screen-printed-electrodes
#19
Tarina Widaningrum, Endrika Widyastuti, Feby Wijaya Pratiwi, Ai Imas Faidoh Fatimah, Patsamon Rijiravanich, Mithran Somasundrum, Werasak Surareungchai
We have constructed biobarcode labels based on 468nm diameter latex spheres. Modification with polyallylamine and then glutaraldehyde was used to attach a high DNA loading, consisting of aminated probe DNA (approx. 1.01×10(2) molecules per sphere) and biobarcode DNA (approx. 1.66×10(4) molecules per sphere). Detection of the biobarcodes was performed by application of a Ag enhancer solution, causing association of the Ag(+) ions with the phosphate groups of the DNA. The deposited Ag was detected by differential pulse voltammetry...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28334954/the-science-and-politics-of-naming-reforming-anatomical-nomenclature-ca-1886-1955
#20
Tatjana Buklijas
Anatomical nomenclature is medicine's official language. Early in their medical studies, students are expected to memorize not only the bodily geography but also the names for all the structures that, by consensus, constitute the anatomical body. The making and uses of visual maps of the body have received considerable historiographical attention, yet the history of production, communication, and reception of anatomical names-a history as long as the history of anatomy itself-has been studied far less. My essay examines the reforms of anatomical naming between the first modern nomenclature, the 1895 Basel Nomina Anatomica (BNA), and the 1955 Nomina Anatomica Parisiensia (NAP, also known as PNA), which is the basis for current anatomical terminology...
April 1, 2017: Journal of the History of Medicine and Allied Sciences
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