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Guojun Cao, Jilie Kong, Zhifang Xing, Yigui Tang, Xinju Zhang, Xiao Xu, Zhihua Kang, Xueen Fang, Ming Guan
Bleeding and thrombosis represent common complications in myeloproliferative neoplasms (MPN) and significantly contribute to morbidity and mortality. Molecular markers, including CALR mutations, were considered not only as diagnostic markers, but also as risk factors for bleeding and thrombosis associated with MPN, especially for patients in remote primary hospitals. We sought to develop an easy-to-use assay for the rapid detection of CALR type 1 (CALR-1) and type 2 (CALR-2) mutations in Philadelphia chromosome-negative MPN patients...
September 18, 2018: Analytica Chimica Acta
Gregory T Forcherio, Jérémy Riporto, Jeremy R Dunklin, Yannick Mugnier, Ronan Le Dantec, Luigi Bonacina, D Keith Roper
This erratum corrects errors in the expressions for ⟨βTMD ⟩ and fitted form of IHRS and a consequent data point in Fig. 4 of a recent Letter [Opt. Lett.42, 5018 (2017)OPLEDP0146-959210.1364/OL.42.005018]. It also supplies data for the reference compound para-nitroaniline (pNA). The correction to ⟨βTMD ⟩ improves experimental agreement from 46% to within 21% of independent scissors-corrected density functional theory (DFT) calculations. Central findings from the original Letter remain intact.
May 15, 2018: Optics Letters
Ping Fu, Wei Sun, Juan Lai, Yi-Hong Shen, Ze Zhang
Two isoforms, Bmpop-a and Bmpop-b, were cloned and characterized, which were found to encode prolyl oligopeptidase (Pop) of the domestic silkworm Bombyx mori. The full lengths of Bmpop-a and Bmpop-b were 2497 and 2508 bp, deducing 707 and 740 amino acids, respectively. Both of them, possessing the typical characteristics of the Pop family of serine proteinase, were detected to be expressed among different tissues and development stages at the transcription and translation levels. Soluble recombinant BmPop-a (rBmPop-a) had oligopeptidase activity toward the substrates, Z-Gly-Pro-pNA, Z-Gly-Pro-AMC and angiotensin I...
May 9, 2018: Gene
Qian Sun, Hong-Yu Zhu, Jun-Fang Wang, Xiao Chen, Ke-Rang Wang, Xiao-Liu Li
A lactose modified pyrene derivative (Py-Lac) was synthesized, with which novel twisted supramolecular nanofibers in diameter about 20 nm were constructed by self-assembly. The nanofibers showed solid-state fluorescence between 400 nm and 650 nm with the maximum emission at 495 nm. Furthermore, its recognition reaction with PNA lectin was investigated by fluorescence spectra and turbidity assays. It is interesting found that the supramolecular assembly as multivalent glycocluster exhibited unique and selectively binding interactions with PNA lectin with the binding constant of 5...
April 23, 2018: Bioorganic Chemistry
Gianluca Accogli, Alberto Maria Crovace, Maria Mastrodonato, Giacomo Rossi, Edda G Francioso, Salvatore Desantis
The effect of a dietary probiotic blend on the carbohydrate composition of mucins secreted by the Brunner's glands in the duodenum of growing-finishing pigs was investigated by means of conventional (periodic acid-Schiff, Alcian Blue pH 2.5, high iron diamine staining) and lectin (15 lectins) histochemistry. Pigs were assigned to two dietary treatments: a control basal diet without the probiotic blend (No-Pro) and a test diet that included the probiotic blend (Pro). Duodenal tissue fragments were fixed in 4% phosphate-buffered-saline-buffered paraformaldehyde, dehydrated through a graded alcohol series, and embedded in paraffin wax...
May 3, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
Meghan L Hackerson, Heidi R Luder, Andrew F Beck, Joseph M Wedig, Pamela C Heaton, Stacey M Frede
OBJECTIVES: To decrease nonadherence rates through the design and implementation of a collaborative prescription management program involving a community pharmacy and a large pediatric primary care center. PRACTICE INNOVATION: Kroger and Cincinnati Children's Pediatric Primary Care Center collaborated to identify and address patients' barriers to filling new prescriptions. After filling new medications for clinic patients, pharmacists telephoned patients to inform them that their prescription was ready and to reinforce the importance of initiating the therapy...
May 2, 2018: Journal of the American Pharmacists Association: JAPhA
Ami B Shah, Isaac Nivar, Diana L Speelman
BACKGROUND: Elevated testosterone (T) is routinely reported as a marker of hyperandrogenemia in rodent models for polycystic ovary syndrome (PCOS). In women with PCOS, elevated serum androstenedione (A4) is associated with more severe phenotypes, including a positive correlation with serum T, DHEAS, free androgen index (FAI), LH, and LH/FSH ratio. Furthermore, A4, along with calculated free T and FAI, was identified as one of the best predictors of PCOS in adult women of all ages (18 to > 50 y)...
2018: PloS One
Glenn P Lobo, Gayle Pauer, Joshua H Lipschutz, Stephanie A Hagstrom
Vitamin A/retinol (ROL) and its metabolites (retinoids) play critical roles in eye development and photoreception. Short-term dietary vitamin A deficiency (VAD) manifests clinically as night blindness, while prolonged VAD is known to cause retinal pigment epithelium (RPE) and photoreceptor degeneration. Therefore, sustained uptake of dietary vitamin A, for ocular retinoid production, is essential for photoreceptor health and visual function. The mechanisms influencing the uptake, storage, and supply of dietary vitamin A, for ocular retinoid production, however, are not fully understood...
2018: Advances in Experimental Medicine and Biology
María Andrea Fiorentino, Fernando Alberto Paolicchi, Carlos Manuel Campero, Claudio G Barbeito
Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B...
2018: Open Veterinary Journal
Oleksandr Zavoiura, Ute Resch-Genger, Oliver Seitz
Detection of pathogenic nucleic acids remains one of the most reliable approaches for the diagnosis of a broad range of diseases. Current PCR-based methods require experienced personnel and cannot be easily used for point-of-care diagnostics, making alternative strategies for the sensitive, reliable, and cost-efficient detection of pathogenic nucleic acids highly desirable. Here, we report an enzyme-free method for the fluorometric detection of RNA that relies on a target-induced fluorophore transfer onto a semiconductor quantum dot (QD), uses PNA probes as selective recognition elements and can be readout with simple and inexpensive equipment...
April 25, 2018: Bioconjugate Chemistry
Lene Bay, Kasper N Kragh, Steffen R Eickhardt, Steen S Poulsen, Lise Mette R Gjerdrum, Khaled Ghathian, Henrik Calum, Magnus S Ågren, Thomas Bjarnsholt
Objective: The bacterial composition and distribution were evaluated in acute standardized epidermal wounds and uninjured skin by a molecular in situ technology benchmarked to conventional culturing. This was done to reveal whether bacterial biofilm is present in acute wounds. Approach: On the buttock of 26 healthy volunteers, 28 suction blisters were made and de-roofed. Four wounds were biopsied immediately after wounding, whereas the remaining 24 wounds were treated daily with sterile deionized water and covered with a moisture-retaining dressing...
April 1, 2018: Advances in Wound Care
Le Wang, Shenfei Zong, Zhuyuan Wang, Ju Lu, Chen Chen, Ruohu Zhang, Yiping Cui
Single molecule localization microscopy (SMLM) is a powerful tool for imaging biological targets at the nanoscale. In this report, we present SMLM imaging of telomeres and centromeres using fluorescence in situ hybridization (FISH). The FISH probes were fabricated by decorating CdSSe/ZnS quantum dots (QDs) with telomere or centromere complementary DNA strands. SMLM imaging experiments using commercially available peptide nucleic acid (PNA) probes labeled with organic fluorophores were also conducted to demonstrate the advantages of using QDs FISH probes...
April 19, 2018: Nanotechnology
Ji-Jie Pang, Zhuo Yang, Roy A Jacoby, Samuel M Wu
Some mammalian rod bipolar cells (RBCs) can receive excitatory chemical synaptic inputs from both rods and cones (DBCR2 ), but anatomical evidence for mammalian cone-RBC contacts has been sparse. We examined anatomical cone-RBC contacts using neurobiotin (NB) to visualize individual mouse cones and standard immuno-markers to identify RBCs, cone pedicles and synapses in mouse and baboon retinas. Peanut agglutinin (PNA) stained the basal membrane of all cone pedicles, and mouse cones were positive for red/green (R/G)-opsin, whereas baboon cones were positive for calbindin D-28k...
April 18, 2018: Journal of Comparative Neurology
Stefano Tomassi, Caterina Ieranò, Maria Emilia Mercurio, Ersilia Nigro, Aurora Daniele, Rosita Russo, Angela Chambery, Ilaria Baglivo, Paolo Vincenzo Pedone, Giuseppina Rea, Maria Napolitano, Stefania Scala, Sandro Cosconati, Luciana Marinelli, Ettore Novellino, Anna Messere, Salvatore Di Maro
Cationic nucleopeptides belong to a family of synthetic oligomers composed by amino acids and nucleobases. Their capability to recognize nucleic acid targets and to cross cellular membranes provided the basis for considering them as novel non-covalent delivery agents for nucleic acid pharmaceuticals. Herein, starting from a 12-mer nucleopeptide model, the number of cationic residues was modulated in order to obtain new nucleopeptides endowed with high solubility in acqueous medium, acceptable bio-stability, low cytotoxicity and good capability to bind nucleic acid...
April 7, 2018: Bioorganic & Medicinal Chemistry
R S Prosser, P L Gillis, D Milani, E A M Holman, H Ikert, D Schissler, J Toito, V Palabrica, J L Parrott, A J Bartlett, V K Balakrishnan
Substituted phenylamine antioxidants (SPAs) are additives in a variety of commercial polymers (e.g., lubricants, plastics, etc.). Based on their physicochemical properties, if SPAs were to enter an aquatic system, they would likely partition into sediment and have the capacity to bioaccumulate in biota. This study investigated the potential of four sediment-associated SPAs, diphenylamine (DPA), N-phenyl-1-naphthalene (PNA), N-(1,3-dimethylbutyl)-N'-phenyl-1,4-phenylenediamine (DPPDA), and 4,4'-methylene-bis[N-sec-butylaniline] (MBA) to accumulate in the tissues of freshwater mussels (Lampsilis siliquoidea) and oligochaete worms (Tubifex tubifex)...
April 11, 2018: Ecotoxicology
Jessica Sayers, Richard J Payne, Nicolas Winssinger
The development of a rapid and chemoselective selenocystine-selenoester peptide ligation that operates at nanomolar reactant concentrations has been developed by utilising PNA templation. Kinetic analysis of the templated peptide ligation revealed that the selenocystine-selenoester reaction was 10 times faster than traditional native chemical ligation at cysteine and to our knowledge is the fastest templated ligation reaction reported to date. The efficiency and operational simplicity of this technology is highlighted through the formation of hairpin molecular architectures and in a novel paper-based lateral flow assay for the rapid and sequence specific detection of oligonucleotides, including miRNA in cell lysates...
January 28, 2018: Chemical Science
E Arriola, A Paredes-Lario, R García-Gomez, P Diz-Tain, M Constenla, C García-Girón, G Márquez, M Reck, G López-Vivanco
PURPOSE: The analysis of epidermal growth factor receptor (EGFR) mutations in many patients with advanced non-small-cell lung cancer (aNSCLC) has provided the opportunity for successful treatment with specific, targeted EGFR tyrosine kinase inhibitors. However, this therapeutic decision may be challenging when insufficient tumor tissue is available for EGFR mutation testing. Therefore, blood surrogate samples for EGFR mutation analysis have been suggested. METHODS: Data were collected from the Spanish cohort of patients in the large, non-interventional, diagnostic ASSESS study (NCT01785888) evaluating the utility of circulating free tumor-derived DNA from plasma for EGFR mutation testing...
April 5, 2018: Clinical & Translational Oncology
Dalia Ibrahim
The glycoconjugates pattern of acidic secretions and distribution of chemosensory cells (SCCs) in the respiratory mucosa of dromedary camels were analyzed so as to identify their functional role. Secretions of the goblet cells and mucous glandular cells were analyzed to evaluate the variety of sugar chains, focusing on the acidic glycoconjugates. Using lectin histochemistry, WGA, STL, DBA, SBA, VVA and RCA-120 intensely bound to the goblet cells. PNA and ECL labeled the goblet cells with moderate intensity...
April 2018: Tissue & Cell
Seyed Amir Zarei, Keivan Akhtari, Mohammad Piltan, Shaaban M Kamel, Joel T Mague
The dimer of 9-anthraldehyde, namely heptacyclo[,9 .03,8 .011,16 .017,22 .023,28 ]octacosa-3,5,7,11,13,15,17(22),18,20,23(28),24,26-dodecaene-1,9-carbaldehyde, C30 H20 O2 , has been synthesized by refluxing an ethanol solution in the presence of M(ClO4 )2 and 1,3-diaminopropan-2-ol (M = Co2+ or Cu2+ ). Its structure has been determined by single-crystal X-ray diffraction, showing it to be a new polymorph, referred to as polymorph II, in the monoclinic space group P21 /n. It is compared with the previously reported triclinic modification [Ehrenberg (1968)...
April 1, 2018: Acta Crystallographica. Section C, Structural Chemistry
Mauro Sb Silva, Melanie Prescott, Rebecca E Campbell
Androgen excess is a hallmark of polycystic ovary syndrome (PCOS), a prevalent yet poorly understood endocrine disorder. Evidence from women and preclinical animal models suggests that elevated perinatal androgens can elicit PCOS onset in adulthood, implying androgen actions in both PCOS ontogeny and adult pathophysiology. Prenatally androgenized (PNA) mice exhibit a robust increase of progesterone-sensitive GABAergic inputs to gonadotropin-releasing hormone (GnRH) neurons implicated in the pathogenesis of PCOS...
April 5, 2018: JCI Insight
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