Nishaben M Patel, Meenakshi Sundaram Aravintha Siva, Ruchi Kumari, Dipeshwari J Shewale, Ashim Rai, Michael Ritt, Prerna Sharma, Subba Rao Gangi Setty, Sivaraj Sivaramakrishnan, Virupakshi Soppina
Endocytic recycling is a complex itinerary, critical for many cellular processes. Membrane tubulation is a hallmark of recycling endosomes (REs), mediated by KIF13A, a kinesin-3 family motor. Understanding the regulatory mechanism of KIF13A in RE tubulation and cargo recycling is of fundamental importance but is overlooked. Here, we report a unique mechanism of KIF13A dimerization modulated by Rab22A, a small guanosine triphosphatase, during RE tubulation. A conserved proline between neck coil-coiled-coil (NC-CC1) domains of KIF13A creates steric hindrance, rendering the motors as inactive monomers...
February 2021: Science Advances