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https://www.readbyqxmd.com/read/26725201/bloc-1-brings-together-the-actin-and-microtubule-cytoskeletons-to-generate-recycling-endosomes
#1
Cédric Delevoye, Xavier Heiligenstein, Léa Ripoll, Floriane Gilles-Marsens, Megan K Dennis, Ricardo A Linares, Laura Derman, Avanti Gokhale, Etienne Morel, Victor Faundez, Michael S Marks, Graça Raposo
Recycling endosomes consist of a tubular network that emerges from vacuolar sorting endosomes and diverts cargoes toward the cell surface, the Golgi, or lysosome-related organelles. How recycling tubules are formed remains unknown. We show that recycling endosome biogenesis requires the protein complex BLOC-1. Mutations in BLOC-1 subunits underlie an inherited disorder characterized by albinism, the Hermansky-Pudlak Syndrome, and are associated with schizophrenia risk. We show here that BLOC-1 coordinates the kinesin KIF13A-dependent pulling of endosomal tubules along microtubules to the Annexin A2/actin-dependent stabilization and detachment of recycling tubules...
January 11, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/26680000/structural-correlation-of-the-neck-coil-with-the-coiled-coil-cc1-forkhead-associated-fha-tandem-for-active-kinesin-3-kif13a
#2
Jinqi Ren, Lin Huo, Wenjuan Wang, Yong Zhang, Wei Li, Jizhong Lou, Tao Xu, Wei Feng
Processive kinesin motors often contain a coiled-coil neck that controls the directionality and processivity. However, the neck coil (NC) of kinesin-3 is too short to form a stable coiled-coil dimer. Here, we found that the coiled-coil (CC1)-forkhead-associated (FHA) tandem (that is connected to NC by Pro-390) of kinesin-3 KIF13A assembles as an extended dimer. With the removal of Pro-390, the NC-CC1 tandem of KIF13A unexpectedly forms a continuous coiled-coil dimer that can be well aligned into the CC1-FHA dimer...
February 12, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25624392/a-novel-assay-reveals-preferential-binding-between-rabs-kinesins-and-specific-endosomal-subpopulations
#3
Marvin Bentley, Helena Decker, Julie Luisi, Gary Banker
Identifying the proteins that regulate vesicle trafficking is a fundamental problem in cell biology. In this paper, we introduce a new assay that involves the expression of an FKBP12-rapamycin-binding domain-tagged candidate vesicle-binding protein, which can be inducibly linked to dynein or kinesin. Vesicles can be labeled by any convenient method. If the candidate protein binds the labeled vesicles, addition of the linker drug results in a predictable, highly distinctive change in vesicle localization. This assay generates robust and easily interpretable results that provide direct experimental evidence of binding between a candidate protein and the vesicle population of interest...
February 2, 2015: Journal of Cell Biology
https://www.readbyqxmd.com/read/24706892/dimerization-of-mammalian-kinesin-3-motors-results-in-superprocessive-motion
#4
Virupakshi Soppina, Stephen R Norris, Aslan S Dizaji, Matt Kortus, Sarah Veatch, Michelle Peckham, Kristen J Verhey
The kinesin-3 family is one of the largest among the kinesin superfamily and its members play important roles in a wide range of cellular transport activities, yet the molecular mechanisms of kinesin-3 regulation and cargo transport are largely unknown. We performed a comprehensive analysis of mammalian kinesin-3 motors from three different subfamilies (KIF1, KIF13, and KIF16). Using Forster resonance energy transfer microscopy in live cells, we show for the first time to our knowledge that KIF16B motors undergo cargo-mediated dimerization...
April 15, 2014: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/24462287/recycling-endosome-tubule-morphogenesis-from-sorting-endosomes-requires-the-kinesin-motor-kif13a
#5
Cédric Delevoye, Stéphanie Miserey-Lenkei, Guillaume Montagnac, Floriane Gilles-Marsens, Perrine Paul-Gilloteaux, Francesca Giordano, François Waharte, Michael S Marks, Bruno Goud, Graça Raposo
Early endosomes consist of vacuolar sorting and tubular recycling domains that segregate components fated for degradation in lysosomes or reuse by recycling to the plasma membrane or Golgi. The tubular transport intermediates that constitute recycling endosomes function in cell polarity, migration, and cytokinesis. Endosomal tubulation and fission require both actin and intact microtubules, but although factors that stabilize recycling endosomal tubules have been identified, those required for tubule generation from vacuolar sorting endosomes (SEs) remain unknown...
February 13, 2014: Cell Reports
https://www.readbyqxmd.com/read/24048253/mbnl1-and-rbfox2-cooperate-to-establish-a-splicing-programme-involved-in-pluripotent-stem-cell-differentiation
#6
Julian P Venables, Laure Lapasset, Gilles Gadea, Philippe Fort, Roscoe Klinck, Manuel Irimia, Emmanuel Vignal, Philippe Thibault, Panagiotis Prinos, Benoit Chabot, Sherif Abou Elela, Pierre Roux, Jean-Marc Lemaitre, Jamal Tazi
Reprogramming somatic cells into induced pluripotent stem cells (iPSCs) has provided huge insight into the pathways, mechanisms and transcription factors that control differentiation. Here we use high-throughput RT-PCR technology to take a snapshot of splicing changes in the full spectrum of high- and low-expressed genes during induction of fibroblasts, from several donors, into iPSCs and their subsequent redifferentiation. We uncover a programme of concerted alternative splicing changes involved in late mesoderm differentiation and controlled by key splicing regulators MBNL1 and RBFOX2...
2013: Nature Communications
https://www.readbyqxmd.com/read/23438369/a-molecular-motor-kif13a-controls-anxiety-by-transporting-the-serotonin-type-1a-receptor
#7
Ruyun Zhou, Shinsuke Niwa, Laurent Guillaud, Ying Tong, Nobutaka Hirokawa
Molecular motors are fundamental to neuronal morphogenesis and function. However, the extent to which molecular motors are involved in higher brain functions remains largely unknown. In this study, we show that mice deficient in the kinesin family motor protein KIF13A (Kif13a(-/-) mice) exhibit elevated anxiety-related behavioral phenotypes, probably because of a reduction in 5HT(1A) receptor (5HT(1A)R) transport. The cell-surface expression level of the 5HT(1A)R was reduced in KIF13A-knockdown neuroblastoma cells and Kif13a(-/-) hippocampal neurons...
February 21, 2013: Cell Reports
https://www.readbyqxmd.com/read/23279019/the-microtubule-motor-protein-kif13a-is-involved-in-intracellular-trafficking-of-the-lassa-virus-matrix-protein-z
#8
Sarah Katharina Fehling, Takeshi Noda, Andrea Maisner, Boris Lamp, Karl-Klaus Conzelmann, Yoshihiro Kawaoka, Hans-Dieter Klenk, Wolfgang Garten, Thomas Strecker
The small matrix protein Z of arenaviruses has been identified as the main driving force to promote viral particle production at the plasma membrane. Although multiple functions of Z in the arenaviral life cycle have been uncovered, the mechanism of intracellular transport of Z to the site of virus budding is poorly understood and cellular motor proteins that mediate Z trafficking remain to be identified. In the present study, we report that the Z protein of the Old World arenavirus Lassa virus (LASV) interacts with the kinesin family member 13A (KIF13A), a plus-end-directed microtubule-dependent motor protein...
February 2013: Cellular Microbiology
https://www.readbyqxmd.com/read/22908316/a-novel-split-kinesin-assay-identifies-motor-proteins-that-interact-with-distinct-vesicle-populations
#9
Brian Jenkins, Helena Decker, Marvin Bentley, Julie Luisi, Gary Banker
Identifying the kinesin motors that interact with different vesicle populations is a longstanding and challenging problem with implications for many aspects of cell biology. Here we introduce a new live-cell assay to assess kinesin-vesicle interactions and use it to identify kinesins that bind to vesicles undergoing dendrite-selective transport in cultured hippocampal neurons. We prepared a library of "split kinesins," comprising an axon-selective kinesin motor domain and a series of kinesin tail domains that can attach to their native vesicles; when the split kinesins were assembled by chemical dimerization, bound vesicles were misdirected into the axon...
August 20, 2012: Journal of Cell Biology
https://www.readbyqxmd.com/read/20798811/gadkin-a-novel-link-between-endosomal-vesicles-and-microtubule-tracks
#10
Tanja Maritzen, Volker Haucke
Different types of endosomal vesicles show distinct distribution patterns within cells. While early endosomes can be found throughout the cell, recycling endosomal vesicles and tubules tend to cluster near the microtubule organizing center in the perinuclear region in most cell types. The molecular mechanisms underlying the steady-state distribution and dynamics of various types of endosomal vesicles has long remained enigmatic. However, during the past decade it has become evident that microtubule-based motor proteins of the kinesin family play a pivotal role in the positioning of endosomes...
July 2010: Communicative & Integrative Biology
https://www.readbyqxmd.com/read/20362085/using-quantitative-pcr-to-identify-kinesin-3-genes-that-are-upregulated-during-growth-arrest-in-mouse-nih3t3-cells
#11
Rikke I Thorsteinsson, Søren T Christensen, Lotte B Pedersen
Most cells in our body form a single primary cilium when entering growth arrest. During the past decade, a number of studies have revealed a key role for primary cilia in coordinating a variety of signaling pathways that control important cellular and developmental processes. Consequently, significant effort has been directed toward the identification of genes involved in ciliary assembly and function. Many candidate ciliary genes and proteins have been identified using large-scale "omics" approaches, including proteomics, transcriptomics, and comparative genomics...
2009: Methods in Cell Biology
https://www.readbyqxmd.com/read/20208530/ptdins-3-p-controls-cytokinesis-through-kif13a-mediated-recruitment-of-fyve-cent-to-the-midbody
#12
Antonia P Sagona, Ioannis P Nezis, Nina Marie Pedersen, Knut Liestøl, John Poulton, Tor Erik Rusten, Rolf I Skotheim, Camilla Raiborg, Harald Stenmark
Several subunits of the class III phosphatidylinositol-3-OH kinase (PI(3)K-III) complex are known as tumour suppressors. Here we uncover a function for this complex and its catalytic product phosphatidylinositol-3-phosphate (PtdIns(3)P) in cytokinesis. We show that PtdIns(3)P localizes to the midbody during cytokinesis and recruits a centrosomal protein, FYVE-CENT (ZFYVE26), and its binding partner TTC19, which in turn interacts with CHMP4B, an endosomal sorting complex required for transport (ESCRT)-III subunit implicated in the abscission step of cytokinesis...
April 2010: Nature Cell Biology
https://www.readbyqxmd.com/read/19841138/ap-1-and-kif13a-coordinate-endosomal-sorting-and-positioning-during-melanosome-biogenesis
#13
Cédric Delevoye, Ilse Hurbain, Danièle Tenza, Jean-Baptiste Sibarita, Stéphanie Uzan-Gafsou, Hiroshi Ohno, Willie J C Geerts, Arie J Verkleij, Jean Salamero, Michael S Marks, Graça Raposo
Specialized cell types exploit endosomal trafficking to deliver protein cargoes to cell type-specific lysosome-related organelles (LROs), but how endosomes are specified for this function is not known. In this study, we show that the clathrin adaptor AP-1 and the kinesin motor KIF13A together create peripheral recycling endosomal subdomains in melanocytes required for cargo delivery to maturing melanosomes. In cells depleted of AP-1 or KIF13A, a subpopulation of recycling endosomes redistributes to pericentriolar clusters, resulting in sequestration of melanosomal enzymes like Tyrp1 in vacuolar endosomes and consequent inhibition of melanin synthesis and melanosome maturation...
October 19, 2009: Journal of Cell Biology
https://www.readbyqxmd.com/read/19841135/it-takes-two-to-tango-to-the-melanosome
#14
COMMENT
Aparna Lakkaraju, Jose Maria Carvajal-Gonzalez, Enrique Rodriguez-Boulan
The role of clathrin adaptor proteins in sorting cargo in the biosynthetic and recycling routes is an area of intense research. In this issue, Delevoye et al. (2009. J. Cell Biol. doi:10.1083/jcb.200907122) show that a close interaction between the clathrin adaptor AP-1 and a kinesin motor KIF13A is essential for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes and for organelle biogenesis.
October 19, 2009: Journal of Cell Biology
https://www.readbyqxmd.com/read/16180235/expression-analysis-of-6p22-genomic-gain-in-retinoblastoma
#15
Marija Orlic, Clarellen E Spencer, Lisa Wang, Brenda L Gallie
To identify gene(s) targeted by 6p22 genomic gain, present in more than 50% retinoblastoma tumors, we used real-time RT-PCR to quantify the expression of seven genes in normal human retina and retinoblastoma. Six genes are located in the quantitative multiplex PCR-defined 0.6 Mb minimal region of gain at 6p22 (DEK, AOF1, TPMT, NHLRC1, KIF13A, and NUP153), and E2F3 is 2 Mb away from the minimal region of gain on 6p22. E2F3, DEK, KIF13A, and NUP153 were most frequently overexpressed in retinoblastoma with 6p genomic gain, compared with the normal adult human retina...
January 2006: Genes, Chromosomes & Cancer
https://www.readbyqxmd.com/read/15615415/-the-effects-of-si-wu-tang-on-serum-protein-of-blood-deficient-mice-induced-by-radiation
#16
Zeng-chun Ma, Yue Gao, Hong-ling Tang, Sheng-qi Wang
OBJECTIVE: To study the effects of Si-Wu-Tang on serum protein of blood deficient mice b y proteomicstechnique and study the enriching and regulating blood mechanism of Si-Wu-Tang on mocular level. METHOD: The blood deficient mice was induced by using a single dose of 3.5 Gy radiation from a 60Cogamma source, and high resolution two-dimensional polyacryamide gel electrophoresis (2-DE), computer-assisted image analysis, and mass spectrometry were used to detect regulated protein by Si-Wu-Tang...
November 2003: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/14532993/identification-and-characterization-of-human-ll5a-gene-and-mouse-ll5a-gene-in-silico
#17
Masuko Katoh, Masaru Katoh
ARCN1, KIAA0638, TREH, DDX6, BLR1, BCL9L, UPK2, DLNB13, DLNB14, RPS25, SBDN, G6PT1, HYOU1, VPS11, HMBS, H2AFX, DPAGT1, KIAA0285, MIZF, ABCG4, NOD9, PDZK2, CBL, MCAM, RNF26, C1QTNF5, MFRP, USP2, THY1, and PVRL1 genes are located within the commonly deleted region of neuroblastoma at human chromosome 11q23.3. Here, we characterized the KIAA0638 gene within the 11q23.3 region by using bioinformatics. Because human KIAA0638 gene was homologous to human LL5B gene encoding LL5beta protein, KIAA0638 was designated LL5A gene encoding LL5alpha protein...
November 2003: International Journal of Oncology
https://www.readbyqxmd.com/read/11861365/genomic-amplification-in-retinoblastoma-narrowed-to-0-6-megabase-on-chromosome-6p-containing-a-kinesin-like-gene-rbkin
#18
Danian Chen, Sanja Pajovic, Allison Duckett, Vivette D Brown, Jeremy A Squire, Brenda L Gallie
All retinoblastomas (RBs) show genomic changes in addition to loss of both RB1 alleles. Quantitative-multiplex PCR analysis identified in 41 of 70 (59%) RBs a 0.6-Mb minimal region of chromosome 6p22 gain from which we cloned the human kinesin gene, RBKIN/KIF13A, by rapid amplification of retinal cDNA. RBKIN is expressed ubiquitously in adult tissues, at low levels in fetal tissues, and at high levels in RB. Antisense RBKIN oligonucleotide reduced the growth rate of RB cell lines. RBKIN and/or another closely linked gene are candidate oncogenes contributing to malignant progression of RB...
February 15, 2002: Cancer Research
https://www.readbyqxmd.com/read/11374900/identification-of-the-human-kif13a-gene-homologous-to-drosophila-kinesin-73-and-candidate-for-schizophrenia
#19
S Jamain, H Quach, M Fellous, T Bourgeron
Several studies have reported significant linkage for schizophrenia on 6p23, with a maximum lod score between D6S274 and D6S285. In this paper, we present a new human kinesin gene localized in this 2-cM interval. This gene, termed KIF13A, belongs to the unc-104/KIF1A kinesin subfamily and represents the orthologue of Drosophila kinesin-73. Several alternative transcripts are differentially expressed in human tissues, probably reflecting differences in cargo binding and transport of corresponding proteins. During early mouse development, its homologue (Kif13A) is expressed essentially in the central nervous system...
May 15, 2001: Genomics
https://www.readbyqxmd.com/read/11106728/a-novel-motor-kif13a-transports-mannose-6-phosphate-receptor-to-plasma-membrane-through-direct-interaction-with-ap-1-complex
#20
T Nakagawa, M Setou, D Seog, K Ogasawara, N Dohmae, K Takio, N Hirokawa
Intracellular transport mediated by kinesin superfamily proteins (KIFs) is a highly regulated process. The molecular mechanism of KIFs binding to their respective cargoes remains unclear. We report that KIF13A is a novel plus end-directed microtubule-dependent motor protein and associates with beta 1-adaptin, a subunit of the AP-1 adaptor complex. The cargo vesicles of KIF13A contained AP-1 and mannnose-6-phosphate receptor (M6PR). Overexpression of KIF13A resulted in mislocalization of the AP-1 and the M6PR...
November 10, 2000: Cell
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