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Mesenchymal stem cell

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https://www.readbyqxmd.com/read/29245116/human-lung-epithelial-cells-a549-epithelial-mesenchymal-transition-induced-by-pva-collagen-nanofiber
#1
Xiuchun Li, Shanshan Yan, Jing Dai, Yi Lu, Yiqun Wang, Man Sun, Jinkang Gong, Yuan Yao
Epithelial-mesenchymal transition (EMT) is a process by which epithelial cells lose their cell-cell contact to become mesenchymal stem cells, which is important on development and embryogenesis, wound healing, and cancer metastasis. This research aims to investigate the effect of topological cue as modulating factor on the EMT by tuning the diameter of electrospinning nanofiber. The cell-nanofiber interaction between human lung epithelial cell A549 and electrospinning nanofibers composed of polyvinyl alcohol (PVA) and type I collagen were investigated...
December 11, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29245051/magnesium-phosphate-ceramics-incorporating-a-novel-indene-compound-promote-osteoblast-differentiation-in%C3%A2-vitro-and-bone-regeneration-in%C3%A2-vivo
#2
Ju Ang Kim, Hui-Suk Yun, Young-Ae Choi, Jung-Eun Kim, So-Young Choi, Tae-Geon Kwon, Young Kyung Kim, Tae-Yub Kwon, Myung Ae Bae, Nak Jeong Kim, Yong Chul Bae, Hong-In Shin, Eui Kyun Park
Incorporating bioactive molecules into synthetic ceramic scaffolds is challenging. In this study, to enhance bone regeneration, a magnesium phosphate (MgP) ceramic scaffold was incorporated with a novel indene compound, KR-34893. KR-34893 induced the deposition of minerals and expression of osteoblast marker genes in primary human bone marrow mesenchymal stem cells (BMSCs) and a mouse osteoblastic MC3T3-E1 cell line. Analysis of the mode of action showed that KR-34893 induced the phosphorylation of MAPK/extracellular signal-regulated kinase and extracellular signal-regulated kinase, and subsequently the expression of bone morphogenetic protein 7, accompanied by SMAD1/5/8 phosphorylation...
December 7, 2017: Biomaterials
https://www.readbyqxmd.com/read/29244789/pigment-epithelium-derived-factor-from-arpe19-promotes-proliferation-and-inhibits-apoptosis-of-human-umbilical-mesenchymal-stem-cells-in-serum-free-medium
#3
Dah-Ching Ding, Yao-Tseng Wen, Rong-Kung Tsai
Clinical expansion of mesenchymal stem cells (MSCs) is hampered by the lack of knowledge regarding how to prevent MSC apoptosis and promote their proliferation in serum-free medium. Our in vitro studies demonstrated that human umbilical cord MSCs (HUCMSCs) underwent apoptosis in the serum-free medium. When HUCMSCs were co-cultured with retinal pigment epithelial cells (ARPE19), however, HUCMSCs exhibited normal growth and morphology in serum-free medium. Their colony formation was promoted by the conditioned medium (CM) of ARPE19 cells on Matrigel...
December 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29243809/obesity-induced-mitochondrial-dysfunction-in-porcine-adipose-tissue-derived-mesenchymal-stem-cells
#4
Yu Meng, Alfonso Eirin, Xiang-Yang Zhu, Hui Tang, Pritha Chanana, Amir Lerman, Andre J van Wijnen, Lilach O Lerman
BACKGROUND: Transplantation of autologous mesenchymal stem cells (MSCs) may be a viable option for treatment of several diseases. MSCs efficacy depends on adequate function of their mitochondria, which might be impaired in a noxious milieu. OBJECTIVES: We hypothesized that obesity compromises MSCs mitochondrial structure and function, possibly via micro-RNA (miRNA)-based mechanisms. METHODS: MSCs were collected from swine abdominal adipose tissue after 16 weeks of Lean or Obese diet (n = 7 each)...
December 15, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29243283/intra-articular-injection-of-autologous-adipose-derived-mesenchymal-stem-cells-in-treatment-of-knee-osteoarthritis
#5
Duško Spasovski, Vesna Spasovski, Zoran Baščarević, Maja Stojiljković, Miša Vreća, Marina Andjelković, Sonja Pavlović
BACKGROUND: Osteoarthritis (OA) is a chronic degenerative joint disease, considered to be the fourth leading cause of disability and the second cause of inability to work in men. Recently, adipose derived mesenchymal stem cells (AD-MSCs) came in focus of regenerative medicine as promising tool for treatment of OA. It has been shown that administration of stem cells into impaired joints results in pain relief and improves quality of life, accompanied with restoration of hyaline articular cartilage...
December 15, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29242640/effects-of-maternal-obesity-on-wharton-s-jelly-mesenchymal-stromal-cells
#6
Heba Badraiq, Aleksandra Cvoro, Antonio Galleu, Marisa Simon, Cristian Miere, Carl Hobbs, Reiner Schulz, Richard Siow, Francesco Dazzi, Dusko Ilic
We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese (BMI = 19-25; n = 7) and obese (BMI ≥ 30; n = 7) donors undergoing elective Cesarean section, we found that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive activity...
December 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29242275/igfbp7-regulates-the-osteogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-via-wnt-%C3%AE-catenin-signaling-pathway
#7
Wei Zhang, Erman Chen, Mo Chen, Chenyi Ye, Yiying Qi, Qianhai Ding, Hang Li, Deting Xue, Xiang Gao, Zhijun Pan
Insulin-like growth factor-binding protein 7 (IGFBP7), a low-affinity IGF binder, may play an important role in bone metabolism. However, its function in osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we investigated its effects on osteogenic differentiation. Overexpression of IGFBP7 enhanced the expression of osteo-specific genes and proteins, and IGFBP7 knockdown decreased osteogenesis-specific markers. More mineral deposits and higher alkaline phosphatase activity were observed after the up-regulation of IGFBP7 Moreover, β-catenin levels were up-regulated by the overexpression of IGFBP7 or the addition of extracellular IGFBP7 protein and were reduced by the depletion of IGFBP7 The increase in osteogenic differentiation due to the overexpression of IGFBP7 was partially decreased by specific Wnt/β-catenin signaling inhibitors...
December 14, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29242161/preparation-of-dexamethasone-loaded-biphasic-calcium-phosphate-nanoparticles-collagen-porous-composite-scaffolds-for-bone-tissue-engineering
#8
Ying Chen, Naoki Kawazoe, Guoping Chen
Although bone is regenerative, its regeneration capacity is limited. For bone defects beyond a critical size, further intervention is required. As an attractive strategy, bone tissue engineering (bone TE) has been widely investigated to repair bone defects. However, the rapid and effective bone regeneration of large non-healing defects is still a great challenge. Multifunctional scaffolds having osteoinductivity and osteoconductivity are desirable to fasten functional bone tissue regeneration. In the present study, biomimetic composite scaffolds of collagen and biphasic calcium phosphate nanoparticles (BCP NPs) with a controlled release of dexamethasone (DEX) and the controlled pore structures were prepared for bone TE...
December 11, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29242152/human-umbilical-cord-derived-mesenchymal-stem-cells-protect-from-hyperoxic-lung-injury-by-ameliorating-aberrant-elastin-remodeling-in-the-lung-of-o2-exposed-newborn-rat
#9
Chen Hou, Danyi Peng, Li Gao, Daiyin Tian, Jihong Dai, Zhengxiu Luo, Enmei Liu, Hong Chen, Lin Zou, Zhou Fu
The incidence and mortality rates of bronchopulmonary dysplasia (BPD) remain very high. Therefore, novel therapies are imminently needed to improve the outcome of this disease. Human umbilical cord-derived mesenchymal stem cells (UC-MSCs) show promising therapeutic effects on oxygen-induced model of BPD. In our experiment, UC-MSCs were intratracheally delivered into the newborn rats exposed to hyperoxia, a well-established BPD model. This study demonstrated that UC-MSCs reduce elastin expression stimulated by 90% O2 in human lung fibroblasts-a (HLF-a), and inhibit HLF-a transdifferentiation into myofibroblasts...
December 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29241918/human-amniotic-fluid-stem-cells-have-better-potential-in-early-second-trimester-of-pregnancy-and-can-be-reprogramed-to-ips
#10
S W Steven Shaw, Po-Jen Cheng, Yao-Lung Chang, An-Shine Chao, Tzu-Hao Wang, Shuenn-Dyh Chang, T'sang-T'ang Hsieh, Kuo-Hsuan Chang
OBJECTIVE: To study the difference of amniotic fluid stem cell potential at different gestational age. MATERIALS AND METHODS: Second trimester amniocentesis was performed during 15 to 22nd week of gestational age in a single medical center from 2015 to 2016. Early second trimester amniotic fluid stem cells (E-AFS) and later one (L-AFS) were defined 15-18th week, and 19-22nd week, respectively. Cell characteristics, surface markers and ability to form induced pluripotent stem cells (iPS) were studied...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29241917/pueraria-mirifica-inhibits-17%C3%AE-estradiol-induced-cell-proliferation-of%C3%A2-human-endometrial-mesenchymal-stem-cells
#11
Ta-Chin Lin, Kai-Hung Wang, An-Pei Kao, Kuo-Hsiang Chuang, Tsung-Cheng Kuo
OBJECTIVE: The notion that the human endometrium may contain a population of stem cells has recently been proposed. The mesenchymal stem cells (MSCs) in the endometrium are believed to be responsible for the remarkable regenerative ability of endometrial cells. Estrogens influence the physiological and pathological processes of several hormone-dependent tissues, such as the endometrium. Pueraria mirifica (PM) is a herbal plant that contains several phytoestrogens, including isoflavones, lignans, and coumestans, and is known to exert an estrogenic effect on animal models...
December 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29241656/adipose-derived-stem-cells-decrease-pain-in-a-rat-model-of-oxaliplatin-induced-neuropathy-role-of-vegf-a-modulation
#12
Lorenzo Di Cesare Mannelli, Barbara Tenci, Laura Micheli, Alessia Vona, Francesca Corti, Matteo Zanardelli, Andrea Lapucci, Ann Maria Clemente, Paola Failli, Carla Ghelardini
Oxaliplatin therapy of colorectal cancer induces a dose-dependent neuropathic syndrome in 50% of patients. Pharmacological treatments may offer limited relief; scientific efforts are needed for new therapeutic approaches. Therefore we evaluated in a preclinical setting the pain relieving properties of mesenchymal stem cells and its secretome. Rat adipose stem cells (rASCs) were administered in a rat model of oxaliplatin-induced neuropathy. A single intravenous injection of rASCs reduced oxaliplatin-dependent mechanical hypersensitivity to noxious and non-noxious stimuli taking effect 1 h after administration, peaking 6 h thereafter and lasting 5 days...
December 11, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29241418/isolation-and-characterization-of-mesenchymal-stromal-cells-based-on-aldehyde-dehydrogenase-activity
#13
Mehdi Najar, Laurent Dollé, Emerence Crompot, Stefaan Verhulst, Leo Van Grunsven, Hélène Busser, Laurence Lagneaux
Mesenchymal stromal cells (MSCs) have particular properties that allow their use as therapeutic strategies for several cell-based applications. Historically, bone marrow (BM)-MSCs are isolated by culture adherence since specific cell surface markers are yet to be developed. This original work aimed to identify and characterize isolating expanded BM-MSCs based on their aldehyde dehydrogenase (ALDH) activity known to be a hallmark of stem cells and relevant for their isolation. We thus isolated by FACS technology two functionally different populations of BM-MSCs depending on their ALDH activity (ALDH+ and ALDH-)...
December 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29241367/differentiation-of-conjunctiva-mesenchymal-stem-cells-into-secreting-islet-beta-cells-on-plasma-treated-electrospun-nanofibrous-scaffold
#14
Samad Nadri, Ghasem Barati, Hossein Mostafavi, Abdolreza Esmaeilzadeh, Seyed Ehsan Enderami
Transplantation of stem cells using biocompatible nanofibrous scaffolds is a promising therapeutic method for treating Diabetic Mellitus. The aim of this study was to derive insulin-producing cells (IPCs) from conjunctiva-derived mesenchymal stem cell (CJMSCs) and to compare the functionality of differentiated IPCs in a three-dimensional (3D) culture with 2D. Furthermore, the effects of hydrophobicity of scaffold on IPCs differentiation were examined. Scanning electron microscopy (SEM), quantitative real times PCR (qPCR), Immunostaining and flow cytometry were used to analyze fabricated scaffold and the presence of IPCs...
December 14, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29241308/infusion-of-porcine-derived-amniotic-fluid-stem-cells-for-treatment-of-experimental-colitis-in-mice
#15
Kun-Yi Lin, Shao-Yu Peng, Chih-Jen Chou, Herng-Sheng Lee, Leou-Chyr Lin, Ru-Yu Pan, Shinn-Chih Wu
Recently, stem cells have offered an alternative treatment for inflammatory bowel disease or colitis to overcome the poor outcomes associated with current therapies. Amniotic fluid-derived stem cells have the potential for the regeneration of impaired organs and the recovery of normal physiologic functions of damaged tissues without ethical concerns or risk of tumor formation. In this work, we aimed to examine the therapeutic effects of infusion of porcine amniotic fluid-derived stem cells (pAFSCs) in dextran sulfate sodium (DSS)-induced colitis in mice...
December 15, 2017: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/29241192/microrna-92a-3p-regulates-aggrecanase-1-and-aggrecanase-2-expression-in-chondrogenesis-and-il-1%C3%AE-induced-catabolism-in-human-articular-chondrocytes
#16
Guping Mao, Peihui Wu, Ziji Zhang, Zhiqi Zhang, Weiming Liao, Yukang Li, Yan Kang
BACKGROUND/AIMS: Aggrecanase-1 (ADAMTS-4) and aggrecanase-2 (ADAMTS-5) are secreted enzymes belonging to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family that play significant roles in the progression of osteoarthritis (OA). Here, we aimed to determine whether the expression of ADAMTS-4/5 in chondrogenesis and inflammation is regulated by microRNA-92a-3p (miR-92a-3p). METHODS: MiR-92a-3p and ADAMTS-4/5 expressions were determined by quantitative polymerase chain reaction (qPCR)...
November 3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29240243/fabrication-and-evaluation-of-3d-printed-bcp-scaffolds-reinforced-with-zro2-for-bone-tissue-applications
#17
Min-Woo Sa, Bao-Ngoc B Nguyen, Rebecca A Moriarty, Timur Kamalitdinov, John P Fisher, Jong Young Kim
Fused deposition modeling (FDM) is a promising 3D printing and manufacturing step to create well interconnected porous scaffold designs from the computer-aided design (CAD) models for the next generation of bone scaffolds. The purpose of this study was to fabricate and evaluate a new biphasic calcium phosphate (BCP) scaffold reinforced with zirconia (ZrO2 ) by a FDM system for bone tissue engineering. The 3D slurry foams with blending agents were successfully fabricated by a FDM system. Blending materials were then removed after the sintering process at high temperature to obtain a targeted BCP/ZrO2 scaffold with the desired pore characteristics, porosity, and dimension...
December 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29239730/directed-differentiation-of-periocular-mesenchyme-from-human-embryonic-stem-cells
#18
Matthew Lovatt, Gary Hin-Fai Yam, Gary S Peh, Alan Colman, N Ray Dunn, Jodhbir S Mehta
Corneal tissue is the most transplanted of all body tissues. Currently, cadaveric donor tissues are used for transplantation. However, a global shortage of transplant grade material has prompted development of alternative, cell-based therapies for corneal diseases. Pluripotent stem cells are attractive sources of cells for regenerative medicine, because large numbers of therapeutically useful cells can be generated. However, a detailed understanding of how to differentiate clinically relevant cell types from stem cells is fundamentally required...
November 16, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/29239597/degradation-of-bioresorbable-mg-4zn-1sr-intramedullary-pins-and-associated-biological-responses-in-vitro-and-in-vivo
#19
Aaron F Cipriano, Jiajia Lin, Alan Lin, Amy Sallee, Belinda Le, Mayra Celene Cortez Alcaraz, Ren-Guo Guan, Gary Botimer, Serkan Inceoğlu, Huinan Liu
This article reports the degradation and biological properties of as-drawn Mg-4Zn-1Sr (designated as ZSr41) and pure Mg (P-Mg) wires as bioresorbable intramedullary pins for bone repair. Specifically, their cytocompatibility with bone marrow derived mesenchymal stem cells (BMSCs) and degradation in vitro, and their biological effects on peri-implant tissues and in vivo degradation in rat tibiae were studied. The as-drawn ZSr41 pins showed a significantly faster degradation than P-Mg in vitro and in vivo. The in vivo average daily degradation rates of both ZSr41 and P-Mg intramedullary pins were significantly greater than their respective in vitro degradation rates, likely because the intramedullary site of implantation is highly vascularized for removal of degradation products...
December 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29239337/globose-basal-cells-for-spinal-cord-regeneration
#20
Durai Murugan Muniswami, Indirani Kanakasabapathy, George Tharion
Spinal cord injury (SCI) is a devastating condition with loss of motor and sensory functions below the injury level. Cell based therapies are experimented in pre-clinical studies around the world. Neural stem cells are located intra-cranially in subventricular zone and hippocampus which are highly invasive sources. The olfactory epithelium is a neurogenic tissue where neurogenesis takes place throughout the adult life by a population of stem/progenitor cells. Easily accessible olfactory neuroepithelial stem/progenitor cells are an attractive cell source for transplantation in SCI...
November 2017: Neural Regeneration Research
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