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Collagen Cross-linking

Jiangli Lang, Qian Zhao, Yuedong He, Xijie Yu
CONTEXT: Lung cancer still remains the leading cause of cancer-related mortality worldwide. Bone is one of preferred metastatic sites for lung cancer cells. So far, both accurate diagnosis and effective treatment of lung cancer bone metastases are difficult. OBJECTIVE: This review aimed to evaluate roles of bone turnover markers (BTMs), microRNAs (miRNAs), dickkopf1 (DKK1) and insulin like growth factor binding protein 3 (IGFBP-3) in lung cancer bone metastases...
April 23, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Paweł Łęgosz, Łukasz Pulik, Piotr Stępiński, Jakub Janowicz, Agnieszka Wirkowska, Andrzej Kotela, Sylwia Sarzyńska, Paweł Małdyk
The aim of this study was to determine the co-occurrence of heterotopic ossifications (HO) and elevated bone biomarkers level (urine type I collagen cross-linked C-telopeptide - CTX-1, serum alkaline phosphatase - ALP) and assess the effectiveness of pharmacological prophylaxis of HO. The study group consisted of (N=54) patients qualified for total hip arthroplasty. In N=19 (35%) patients new HO were formed (48th week vs to baseline). On baseline, the mean CTX-1 value in the HO (-) patients was 8.60+/-5.37 ug/mmol vs HO (+) 11...
March 2018: Annals of Clinical and Laboratory Science
Mohammed Ali Abu Ameerh, Muawyah Dawoud Al Bdour, Maha Al-Till, Muhannad Al Faouri
PURPOSE: To evaluate the efficacy of trans-epithelial corneal collagen cross-linking (CXL) with Iontophoresis among patients with progressive keratoconus. METHODS: It is a prospective interventional study, which is based on 41 eyes of 23 patients, suffering from progressive keratoconus and treated with trans-epithelial corneal cross-linking, using iontophoresis with ETDA and trometamol-enriched riboflavin 5 phosphates 0.1% hypotonic solution (Ricrolin+, Soot Italia SpA, Italy)...
April 19, 2018: International Ophthalmology
Yuka Wakuda, Shohei Nishimoto, Shin-Ichiro Suye, Satoshi Fujita
Collagen hydrogel is a popular extracellular matrix (ECM) material in regenerative medicine and has an isotropic structure. In contrast, native ECM has an anisotropic structure. Electrospinning of collagen dissolved in organic solvents is widely used for fabricating anisotropic collagen nanofibres; however, such fibres are water-soluble and require cross-linking before use as scaffolds for cell culture. Herein, electrospinning using a core-shell nozzle was employed to spin an aqueous acidic solution of collagen and encapsulate it within a shell of polyvinylpyrrolidone (PVP)...
April 19, 2018: Scientific Reports
Farhad Mohammadi, Soliman Mohammadi Samani, Nader Tanideh, Fatemeh Ahmadi
Purpose: Cartilage regeneration by using polymeric scaffolds is a new option for treatment of osteoarthritis. A good scaffold for tissue engineering should copy the characteristics of natural extracellular matrix. The purpose of this study was to make a dosage form with proper reliability and stability for cartilage repair. Methods: Hybrid scaffolds containing different ratios of hyaluronic acid (HA) and collagen were prepared and loaded with prednisolone as anti-inflammatory agent. Two different dosage forms (lyophilized implantable disk and thermo-sensitive gels) were examined...
March 2018: Advanced Pharmaceutical Bulletin
Hui Hong, Bimol C Roy, Meram Chalamaiah, Heather L Bruce, Jianping Wu
Small collagen peptides are associated with various benefits, such as bone and skin health. However, preparation of small collagen peptides from terrestrial vertebrate origins remains a challenge. Here, we show that pretreatment with formic acid enhanced the production of small collagen peptides from spent hen skin. After pretreatment with formic acid, the percentage of small peptides below 2 kDa increased to 48.92% and 43.34% from 33.79% and 36.32% for heat-soluble collagen (HSC) and pepsin-soluble collagen (PSC), respectively...
August 30, 2018: Food Chemistry
Shyamsundar Pal China, Subhashis Pal, Sourav Chattopadhyay, Konica Porwal, Monika Mittal, Sabyasachi Sanyal, Naibedya Chattopadhyay
Modafinil is primarily prescribed for treatment of narcolepsy and other sleep-associated disorders. However, its off-prescription use as a cognition enhancer increased considerably, specially among youths. Given its increasing use in young adults the effect of modafinil on peak bone accrual is an important issue but has never been investigated. Modafinil treatment to young male rats caused trabecular and cortical bone loss in tibia and femur, and reduction in biomechanical strength. Co-treatment of modafinil with alendronate (a drug that suppresses bone resorption) reversed the trabecular bone loss but failed to prevent cortical loss...
April 9, 2018: Toxicology and Applied Pharmacology
Supin Liu, Wenlong Wang, Linlin Yin, Yitang Zhu
Objective: To investigate the relationship between serum level of Apelin-13 and bone mineral density (BMD) as well as other parameters, and determine the influence of Apelin-13 on osteoporosis in patients with Type-2 diabetes mellitus. Methods: Seventy-six patients with Type-2 diabetes mellitus were recruited from Department of Endocrinology of our hospital between January 2013 and July2017. The clinical data, including age, gender, height, weight, body mass index (BMI) and disease duration were recorded for all patients...
January 2018: Pakistan Journal of Medical Sciences Quarterly
Nidhi Bharti, Anil Chandra, A P Tikku, Promila Verma, Ramesh Bharti, Vijay Kumar Shakya, Rhythm Bains
Background: This article highlights the contribution of collagen structure/stability to the bond strength. We hypothesize that induction of cross-linking in dentin collagen fibrils improves dentin collagen stability and thus bond strength with composite also improves. Aims: The aim of the study was to evaluate the effect of collagen cross-linking agents on the shear bond strength of composite resins. Subjects and Methods: One hundred human permanent teeth were randomly divided into three groups: Group I ( n = 20) - no dentin pretreatment done, Group II - dentin pretreatment with 10% sodium ascorbate for 5 min (IIa) and 10 min (IIb), and Group III - dentin pretreatment with 6...
January 2018: Journal of Conservative Dentistry: JCD
Miao Yu, Yingying Han, Jian Li, Lijuan Wang
Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively. The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties. When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185...
April 5, 2018: International Journal of Biological Macromolecules
Nikolaos A Afratis, Mordehay Klepfish, Nikos K Karamanos, Irit Sagi
Progressive loss of organ function in most organs is associated with fibrosis, a tissue state associated with abnormal matrix buildup. If highly progressive, the fibrotic process eventually leads to organ failure and death. Fibrosis is a basic connective tissue lesion defined by the increase in the amount of fibrillar extracellular matrix (ECM) components in a tissue or organ. In addition, intrinsic changes in important structural cells can induce the fibrotic response by regulating the differentiation, recruitment, proliferation and activation of extracellular matrix-producing myofibroblasts...
April 5, 2018: Advanced Drug Delivery Reviews
Boushra Bathish, Rufus Turner, Martina Paumann-Page, Anthony J Kettle, Christine C Winterbourn
Peroxidasin is a heme peroxidase that catalyses the oxidation of bromide by hydrogen peroxide to form an essential sulfilimine crosslink between methionine and hydroxylysine residues in collagen IV. We investigated cross-linking by peroxidasin embedded in extracellular matrix isolated from cultured epithelial cells and its sensitivity to alternative substrates and peroxidase inhibitors. Peroxidasin showed peroxidase activity as measured with hydrogen peroxide and Amplex red. Using a specific mass spectrometry assay that measures NADH bromohydrin, we showed definitively that the enzyme releases hypobromous acid (HOBr)...
April 4, 2018: Archives of Biochemistry and Biophysics
Osamu Kurita, Kaori Umetani, Yasushi Kokean, Hironori Maruyama, Toru Sago, Hiroyuki Iwamoto
Poly-γ-glutamic acid (PGA) was easily phosphorylated by direct addition of phosphorylating agents into the culture medium of Bacillus subtilis (natto). Tetrapolyphosphate salt was the most incorporated into PGA molecules of all used reagents. Phosphorylation occurred at the α-carboxyl side chains of PGA molecule. The amounts of bound phosphate to PGA were dependent on the amounts of added phosphorylating agent. In low molecular weight regions of less than 100 kDa, a cross-linked peak was observed in the phosphorylated PGAs, whereas their peaks at approximately 1000 kDa shifted to a higher molecular weight due to the bound phosphate...
April 6, 2018: World Journal of Microbiology & Biotechnology
Xiaoyu Zhang, Jing Zhao, Meiyan Li, Mi Tian, Yang Shen, Xingtao Zhou
Previous studies investigating the effectiveness of conventional corneal collagen cross-linking (CXL) and transepithelial CXL in keratoconus treatment have reported conflicting outcomes. Therefore, we conducted a meta-analysis to compare the effectiveness of these treatments. We searched MEDLINE, EMBASE, and Cochrane Central Register of Controlled Trials for prospective randomized controlled trials (RCTs) with no restrictions. We included visual acuity (corrected distance visual acuity, uncorrected distance visual acuity) and corneal keratometry (K) as primary outcome parameters, and spherical equivalent, central corneal thickness (CCT), and endothelial cell density, as secondary parameters...
2018: PloS One
Tobias Jost, Alexander Zipprich, Marcus A Glomb
Methylglyoxal is a major 1,2-dicarbonyl compound in vivo and leads to nonenzymatic protein modifications, known as advanced glycation endproducts. Especially long-lived proteins like collagen are prone to changes of the mechanical or biological function, respectively, by accumulation of Maillard-derived modifications. Specifically, the resulting nonenzymatic cross-link structures in parallel to the natural maturation process of collagen fibrils lead to complications with age or during disease. A novel lysine-lysine amide cross-link derived from methylglyoxal, 2,15-diamino-8-methyl-9-oxo-7,10-diaza-1,16-hexadecanedioic acid, named MOLA, was synthesized and identified in vitro and in vivo...
April 18, 2018: Journal of Agricultural and Food Chemistry
Jadwiga Ambroszkiewicz, Magdalena Chełchowska, Katarzyna Szamotulska, Grażyna Rowicka, Witold Klemarczyk, Małgorzata Strucińska, Joanna Gajewska
BACKGROUND & AIMS: Measurements of bone mineral density (BMD) reflect bone status but not the dynamics of bone turnover. Biochemical markers, which show global skeletal activity, were validated for the assessment of bone formation and resorption processes. Adipokines also play a significant role in the regulation of bone metabolism. OBJECTIVE: To assess body composition, bone mineral density, bone turnover markers and adipokine levels in relation to vegetarian and omnivorous diets...
March 23, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Penelope J Kallis, Adam J Friedman
Chronic wounds, such as pressure ulcers, diabetic foot ulcers, and venous leg ulcers, are associated with high costs, poor quality of life, and significant morbidity and mortality. A chronic wound develops when progression through the normal phases of wound healing goes awry, creating a hostile environment with elevated levels of pro-inflammatory cytokines, increased matrix metalloproteinases (MMPs), destruction of extracellular matrix (ECM) components, and diminished activity of growth factors and other soluble mediators...
April 1, 2018: Journal of Drugs in Dermatology: JDD
Lenore Lamanna, Patricia E Moran
Diverticular disease includes diverticulosis, which are sac protrusions of the intestinal mucosa, and diverticulitis, inflammation of the diverticula. Diverticular disease is listed as one of the top 10 leading physician diagnoses for gastrointestinal disorders in outpatient clinic visits in the United States. There are several classifications of diverticular disease ranging from asymptomatic diverticulosis to diverticulitis with complications. Several theories are linked to the development of diverticula which includes the physiology of the colon itself, collagen cross-linking, and recently challenged, low-fiber intake...
March 2018: Gastroenterology Nursing: the Official Journal of the Society of Gastroenterology Nurses and Associates
Betul Seher Uysal, Derya Yaman, Ozge Sarac, Emine Akcay, Nurullah Cagil
PURPOSE: To report on a keratoconus (KC) patient with Axenfeld-Rieger syndrome (ARS) who developed sterile keratitis after accelerated corneal collagen cross-linking (CXL). METHODS: An 18-year-old patient with ARS and KC who had previously undergone intrastromal ring segment implantation underwent accelerated CXL (9 mW/cm2  UVA intensity for 10 min). RESULTS: After uneventful surgery, the patient presented with severe photophobia, redness of the eye, and decreased vision 72 h following the procedure...
March 28, 2018: International Ophthalmology
Tarek Saleh, Ebtehal Ahmed, Lina Yu, Kamal Hussein, Kyung-Mee Park, Yun-Suk Lee, Byung-Jae Kang, Ki-Young Choi, Sooyoung Choi, Kyung-Sun Kang, Heung-Myong Woo
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide...
March 27, 2018: Artificial Cells, Nanomedicine, and Biotechnology
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