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Collagen Cross-linking

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https://www.readbyqxmd.com/read/28087486/enhanced-articular-cartilage-by-human-mesenchymal-stem-cells-in-enzymatically-mediated-transiently-rgds-functionalized-collagen-mimetic-hydrogels
#1
Paresh A Parmar, Jean-Philippe St-Pierre, Lesley W Chow, Christopher D Spicer, Violet Stoichevska, Yong Y Peng, Jerome A Werkmeister, John A M Ramshaw, Molly M Stevens
: Recapitulation of the articular cartilage microenvironment for regenerative medicine applications faces significant challenges due to the complex and dynamic biochemical and biomechanical nature of native tissue. Towards the goal of biomaterial designs that enable the temporal presentation of bioactive sequences, recombinant bacterial collagens such as Streptococcal collagen-like 2 (Scl2) proteins can be employed to incorporate multiple specific bioactive and biodegradable peptide motifs into a single construct...
January 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28087216/denosumab-and-alendronate-treatment-in-patients-with-back-pain-due-to-fresh-osteoporotic-vertebral-fractures
#2
Tomoko Tetsunaga, Tomonori Tetsunaga, Keiichiro Nishida, Masato Tanaka, Yoshihisa Sugimoto, Tomoyuki Takigawa, Yoshitaka Takei, Toshifumi Ozaki
INTRODUCTION: Denosumab specifically inhibits the receptor activator for nuclear factor-kappa B ligand (RANKL), and prevents osteoporotic fractures. Several reports have analyzed the effects of denosumab and alendronate alone on bone mineral density (BMD) or reduction of fracture risk. The objective of this study was to analyze the effects of antiresorptive osteoporosis pharmacotherapy on pain relief in patients with fresh vertebral fracture. METHODS: This retrospective, single-center study included 80 patients (10 males, 70 females) with fresh osteoporotic vertebral fractures treated using denosumab at a dose of 60 mg subcutaneously every 6 months (40 patients) or alendronate at a dose of 35 mg orally every week (40 patients) for 6 months in our hospital...
January 10, 2017: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/28071973/collagen-cross-linking-biophysical-biochemical-and-biological-response-analysis
#3
L Delgado, Kieran Fuller, Dimitrios I Zeugolis
Extracted forms of collagen are subjected to chemical cross-linking to enhance their stability. However, traditional cross-linking approaches are associated with toxicity and inflammation. This work investigates the stabilisation capacity, cytotoxicity and inflammatory response of collagen scaffolds cross-linked with glutaraldehyde, carbodiimide, 4-arm polyethylene glycol succinimidyl glutarate, genipin and oleuropein. Although all cross-linking methods reduced free amine groups, variable data were obtained with respect to denaturation temperature, resistance to collagenase digestion and mechanical properties...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28068449/repeated-cross-linking-after-a-short-time-does-not-provide-any-additional-biomechanical-stiffness-in-the-mouse-cornea-in-vivo
#4
David Tabibian, Sabine Kling, Arthur Hammer, Olivier Richoz, Farhad Hafezi
PURPOSE: To study whether repeated collagen cross-linking (CXL) performed in vivo in mice shows an additive effect on mechanical corneal stiffness. METHODS: In this experimental study, epithelium-off CXL was performed in a total of 18 eyes from male C57BL/6 mice, with 0.27%-riboflavin solution applied for 20 minutes, followed by ultraviolet-A (UVA) irradiation (365 nm, 9mW/cm(2)) for 2:50 minutes (fluence 1.53 J/cm(2)). CXL was performed as either a single (1×CXL) or a repeated (2×CXL) treatment...
January 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28064012/effect-of-a-novel-quaternary-ammonium-silane-on-dentin-protease-activities
#5
D Umer, C K Y Yiu, M F Burrow, L-N Niu, F R Tay
OBJECTIVES: Demineralized dentin collagen release C-terminal cross-linked telopeptide (ICTP) and C-terminal peptide (CTX) during degradation. The present study evaluated the effects of dentin pre-treatment with K21, a quaternary ammonium silane (QAS), on matrix metalloproteinase (MMP) and cathepsin K-mediated collagen degradation. METHODS: Dentin beams were demineralized with 10% H3PO4 for 24h. After baseline dry mass measurements, the beams were divided into 5 groups (N=10) according to protease inhibitors...
January 4, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28060764/loxl4-knockdown-enhances-tumor-growth-and-lung-metastasis-through-collagen-dependent-extracellular-matrix-changes-in-triple-negative-breast-cancer
#6
Sul Ki Choi, Hoe Suk Kim, Tiefeng Jin, Woo Kyung Moon
Lysyl oxidase (LOX) family genes catalyze collagen cross-link formation. To determine the effects of lysyl oxidase-like 4 (LOXL4) expression on breast tumor formation and metastasis, we evaluated primary tumor growth and lung metastasis in mice injected with LOXL4-knockdown MDA-MB-231 triple-negative human breast cancer cells. In addition, we analyzed overall survival in breast cancer patients based on LOXL4 expression using a public online database. In the mouse xenograft model, LOXL4 knockdown increased primary tumor growth and lung colonization as well as collagen I and IV, lysine hydroxylase 1 and 2, and prolyl 4-hydroxylase subunit alpha 1 and 2 levels...
2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28060061/conventional-and-iontophoresis-corneal-cross-linking-for-keratoconus-efficacy-and-assessment-by-optical-coherence-tomography-and-confocal-microscopy
#7
Léa Jouve, Vincent Borderie, Otman Sandali, Cyrille Temstet, Elena Basli, Laurent Laroche, Nacim Bouheraoua
PURPOSE: To compare the efficacy, safety, and microstructural corneal changes during 2 years after conventional corneal collagen cross-linking (C-CXL) and transepithelial corneal CXL by iontophoresis (I-CXL) for keratoconus. METHODS: Eighty eyes of 80 patients with progressive keratoconus were treated by C-CXL (n = 40) or I-CXL (n = 40). Patients were investigated before surgery and 1, 3, 6, 12, and 24 months after treatment. We measured central corneal thickness and maximal simulated keratometry values (Kmax) and performed specular microscopy and in vivo confocal microscopy at each time point...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060060/ocular-surface-disease-parameters-after-collagen-cross-linking-for-keratoconus
#8
José I Recalde, Arantxa Acera, Iñaki Rodríguez-Agirretxe, Miguel A Sánchez-Tena, Juan San-Cristóbal, Juan A Durán
PURPOSE: To analyze the variations in tear quantity and quality after corneal collagen cross-linking (CXL) in patients with keratoconus. METHODS: This is a prospective observational study of 24 eyes with keratoconus that underwent epithelium-off CXL. The variables studied were tear film osmolarity, subjective symptoms (Ocular Surface Disease Index), tear breakup time, Schirmer test (SCH) score, tear clearance, fluorescein staining, and lissamine green staining. Variables were determined preoperatively and 3, 6, and 12 months after CXL...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060059/efficacy-of-intrastromal-corneal-ring-segments-combined-with-flash-collagen-cross-linking-in-keratoconus
#9
Carlos H Gordillo, Juan C Grandin, Roger Zaldivar, Roberto Zaldivar, Adriana C Lotfi
PURPOSE: To evaluate outcomes, efficacy, and safety of intrastromal corneal ring segment implantation for treatment of keratoconus combined with collagen cross-linking (CXL) with 1-year follow-up. METHODS: One hundred nineteen eyes of 82 patients with keratoconus (37 females) underwent femtosecond (Intralase FS; Abbott Medical Optics, Inc) laser-assisted intrastromal corneal ring segment implantation (INTRASEG; Gamma Vision) with flash CXL (18 mW/cm for 5 minutes)...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060058/corneal-collagen-cross-linking-for-keratoconus-in-pediatric-patients-long-term-results
#10
Prema Padmanabhan, Sudhir Rachapalle Reddi, Rama Rajagopal, Radhika Natarajan, Geetha Iyer, Bhaskar Srinivasan, Niveditha Narayanan, Meena Lakshmipathy, Shweta Agarwal
PURPOSE: To report the long-term outcome of corneal collagen cross-linking (CXL) for progressive keratoconus in pediatric patients. METHODS: "Epithelium-off" CXL was performed in pediatric eyes with progressive keratoconus. Spectacle-corrected distance visual acuity (CDVA), retinoscopy, topography, and tomography were documented preoperatively and postoperatively at 3 months, 6 months, 1 year, and annually thereafter. RESULTS: A total of 377 eyes of 336 pediatric patients aged 8 to 18 years with progressive keratoconus underwent CXL...
February 2017: Cornea
https://www.readbyqxmd.com/read/28057575/the-preparation-of-poly-%C3%AE-glutamic-acid-nhs-ester-as-a-natural-cross-linking-agent-of-collagen
#11
Junhui Yang, Cuicui Ding, Liulian Huang, Min Zhang, Lihui Chen
γ-PGA-NHS ester, which was prepared using poly(γ-glutamic acid) (γ-PGA) and N-hydroxysuccinimide (NHS) as the raw materials, was synthesized to be a novel cross-linker of collagen. Fourier transform infrared spectra analysis suggested that the products displayed the characteristic absorption peak of ester. Results from nuclear magnetic resonance analysis indicated that the esterification degree of γ-PGA-NHS ester was increased with the increase of NHS. Modified collagen was prepared and characterized. The results of circular dichroism analysis indicated modified collagen retained the triple helix structure of natural collagen...
January 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28056723/chronic-treatment-with-minoxidil-induces-elastic-fiber-neosynthesis-and-functional-improvement-in-the-aorta-of-aged-mice
#12
Marion Coquand-Gandit, Marie Paule Jacob, Wassim Fhayli, Beatriz Romero Ruiz de la Hermosa, Miglena Georgieva, Stéphanie Bouillot, Eric Estève, Jean-Pierre Andrieu, Sandrine Brasseur, Sophie Bouyon, Natalio Garcia-Honduvilla, Philippe Huber, Julia Bujan, Milena Atanasova, Gilles Faury
Normal arterial aging processes involve vascular cell dysfunction associated with wall stiffening, the latter being due to progressive elastin and elastic fiber degradation, as well as elastin and collagen cross-linking by advanced glycation end-products (AGE). These processes progressively lead to cardiovascular dysfunction during aging. Elastin is only synthesized during late gestation and childhood, and further degradation occurring throughout adulthood cannot be physiologically compensated by replacement of altered material...
January 5, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28036074/lysyl-oxidase-and-the-tumor-microenvironment
#13
REVIEW
Tong-Hong Wang, Shih-Min Hsia, Tzong-Ming Shieh
The lysyl oxidase (LOX) family of oxidases contains a group of extracellular copper-dependent enzymes that catalyze the cross-linking of collagen and elastin by oxidation, thus maintaining the rigidity and structural stability of the extracellular matrix (ECM). Aberrant expression or activation of LOX alters the cellular microenvironment, leading to many diseases, including atherosclerosis, tissue fibrosis, and cancer. Recently, a number of studies have shown that LOX is overexpressed in most cancers and that it is involved in the regulation of tumor progression and metastasis...
December 29, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28035498/accelerated-versus-conventional-corneal-collagen-cross-linking-in-patients-with-keratoconus-an-intrapatient-comparative-study
#14
Mohammad Mehdi Sadoughi, Bahram Einollahi, Alireza Baradaran-Rafii, Danial Roshandel, Hamidreza Hasani, Mehrdad Nazeri
PURPOSE: To compare the outcomes of the conventional and accelerated corneal collagen cross-linking (CXL) in patients with bilateral progressive keratoconus (KC). METHODS: Fifteen consecutive patients with bilateral progressive KC were enrolled. In each patient, the fellow eyes were randomly assigned to the conventional CXL (3 mW/cm(2) for 30 min) or accelerated CXL (ACXL) (9 mW/cm(2) for 10 min) groups. Manifest refraction; uncorrected and corrected distant visual acuity; maximum and mean keratometry; corneal hysteresis and corneal resistance factor; endothelial cell density and morphology; central corneal thickness; and wavefront aberrations were measured before and 12 months after the CXL...
December 29, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/28033029/higher-platelet-reactivity-and-platelet-monocyte-complex-formation-in-gram-positive-sepsis-compared-to-gram-negative-sepsis
#15
Rahajeng N Tunjungputri, Wouter van de Heijden, Rolf T Urbanus, Philip G de Groot, Andre van der Ven, Quirijn de Mast
Platelets may play a role in the high risk for vascular complications in Gram-positive sepsis. We compared the platelet reactivity of 15 patients with Gram-positive sepsis, 17 with Gram-negative sepsis and 20 healthy controls using a whole blood flow cytometry-based assay. Patients with Gram-positive sepsis had the highest median fluorescence intensity (MFI) of the platelet membrane expression of P-selectin upon stimulation with high dose adenosine diphosphate (ADP; P = 0.002 vs. Gram-negative and P = 0.005 vs...
December 29, 2016: Platelets
https://www.readbyqxmd.com/read/28031696/under-flap-stromal-bed-cxl-for-early-post-lasik-ectasia-a-novel-treatment-technique
#16
Avi Wallerstein, Eser Adiguzel, Mathieu Gauvin, Nima Mohammad-Shahi, Mark Cohen
PURPOSE: Collagen cross-linking (CXL) for post-laser-assisted in situ keratomileusis (LASIK) ectasia (PLE) is traditionally performed either epi-on or epi-off on the corneal surface. This study describes a novel technique in treating early PLE with under-flap CXL (ufCXL) to the stromal bed and reports on 6-month outcomes. PATIENTS AND METHODS: Case series of seven patients (eight eyes) with topography-diagnosed early PLE treated with ufCXL. Inclusion criteria were early, mild PLE defined as new-onset postoperative manifest refraction cylinder ≤1...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28031082/association-between-geranylgeranyl-pyrophosphate-synthase-gene-polymorphisms-and-bone-phenotypes-and-response-to-alendronate-treatment-in-chinese-osteoporotic-women
#17
Lan-Wen Han, Dou-Dou Ma, Xiao-Jie Xu, Fang Lü, Yi Liu, Wei-Bo Xia, Yan Jiang, Ou Wang, Xiao-Ping Xing, Mei Li
Objective To investigate the relationship between geranylgeranyl pyrophosphate synthase (GGPPS) gene polymorphisms and bone response to alendronate in Chinese osteoporotic women.Methods A total of 639 postmenopausal women with osteoporosis or osteopenia were included and randomly received treatment of low dose (70 mg per two weeks) or standard dose (70 mg weekly) of alendronate for one year. The six tag single nucleotide polymorphisms of GGPPS gene were identified. Bone mineral density (BMD), serum cross-linked C-telopeptide of type I collagen (β-CTX), and total alkaline phosphatase (ALP) were measured before and after treatment...
March 20, 2016: Chinese Medical Sciences Journal, Chung-kuo i Hsüeh K'o Hsüeh Tsa Chih
https://www.readbyqxmd.com/read/28030556/hepatocyte-hypoxia-inducible-factor-1-mediates-the-development-of-liver-fibrosis-in-a-mouse-model-of-nonalcoholic-fatty-liver-disease
#18
Omar A Mesarwi, Mi-Kyung Shin, Shannon Bevans-Fonti, Christina Schlesinger, Janet Shaw, Vsevolod Y Polotsky
BACKGROUND: Obstructive sleep apnea (OSA) is associated with the progression of non-alcoholic fatty liver disease (NAFLD) to steatohepatitis and fibrosis. This progression correlates with the severity of OSA-associated hypoxia. In mice with diet induced obesity, hepatic steatosis leads to liver tissue hypoxia, which worsens with exposure to intermittent hypoxia. Emerging data has implicated hepatocyte cell signaling as an important factor in hepatic fibrogenesis. We hypothesized that hepatocyte specific knockout of the oxygen sensing α subunit of hypoxia inducible factor-1 (HIF-1), a master regulator of the global response to hypoxia, may be protective against the development of liver fibrosis...
2016: PloS One
https://www.readbyqxmd.com/read/28024102/elevated-ischaemia-associated-lysyl-oxidase-activity-in-delayed-graft-failure-6-12%C3%A2-months-after-renal-transplantation
#19
Zhi Chen, Yiyang Li, Hongmei Xu, Fuzhe Ma, Jia Li, Lei Zhao, Ying Xu
What is the central question of this study? What potential biochemical changes are associated with renal parenchyma 6-12 months after renal transplantation and delayed graft failure? What is the main finding and its importance? Tissue fibrosis, mediated by tissue ischaemia-induced induction of hypoxia-inducible factor-1α and fibronectin and consequent activation of lysyl oxidase, is a major underlying pathophysiological mechanism that contributes to delayed graft failure several months after renal transplantation...
November 7, 2016: Experimental Physiology
https://www.readbyqxmd.com/read/28018671/accelerated-corneal-collagen-cross-linking-using-topography-guided-uv-a-energy-emission-preliminary-clinical-and-morphological-outcomes
#20
Cosimo Mazzotta, Antonio Moramarco, Claudio Traversi, Stefano Baiocchi, Alfonso Iovieno, Luigi Fontana
Purpose. To assess the clinical and morphological outcomes of topography-guided accelerated corneal cross-linking. Design. Retrospective case series. Methods. 21 eyes of 20 patients with progressive keratoconus were enrolled. All patients underwent accelerated cross-linking using an ultraviolet-A (UVA) exposure with an energy release varying from 7.2 J/cm(2) up to 15 J/cm(2), according to the topographic corneal curvature. Uncorrected (UDVA) and corrected (CDVA) distance visual acuity, topography, in vivo confocal microscopy (IVCM), and anterior segment optic coherence tomography (AS-OCT) were evaluated preoperatively and at the 1, 3, 6, and 12 months postoperatively...
2016: Journal of Ophthalmology
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