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Collagen Cross-linking

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https://www.readbyqxmd.com/read/29046415/skeletal-stiffening-in-an-amphibious-fish-out-of-water-is-a-response-to-increased-body-weight
#1
Andy J Turko, Dietmar Kültz, Douglas Fudge, Roger P Croll, Frank M Smith, Matthew R Stoyek, Patricia A Wright
Terrestrial animals must support their bodies against gravity, while aquatic animals are effectively weightless because of buoyant support from water. Given this evolutionary history of minimal gravitational loading of fishes in water, it has been hypothesized that weight-responsive musculoskeletal systems evolved during the tetrapod invasion of land and are thus absent in fishes. Amphibious fishes, however, experience increased effective weight when out of water - are these fishes responsive to gravitational loading? Contrary to the tetrapod-origin hypothesis, we found that terrestrial acclimation reversibly increased gill arch stiffness (∼60% increase) in the amphibious fish Kryptolebias marmoratus when loaded normally by gravity, but not under simulated microgravity...
October 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29045803/chitosan-based-trilayer-scaffold-for-multitissue-periodontal-regeneration
#2
E M Varoni, S Vijayakumar, E Canciani, A Cochis, L De Nardo, G Lodi, L Rimondini, M Cerruti
Periodontal regeneration is still a challenge for periodontists and tissue engineers, as it requires the simultaneous restoration of different tissues-namely, cementum, gingiva, bone, and periodontal ligament (PDL). Here, we synthetized a chitosan (CH)-based trilayer porous scaffold to achieve periodontal regeneration driven by multitissue simultaneous healing. We produced 2 porous compartments for bone and gingiva regeneration by cross-linking with genipin either medium molecular weight (MMW) or low molecular weight (LMW) CH and freeze-drying the resulting scaffolds...
October 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29044707/the-effect-of-vitamin-d-supplementation-on-bone-metabolic-markers-in-chronic-kidney-disease
#3
Ashok Kumar Yadav, Vivek Kumar, Vinod Kumar, Debasish Banerjee, Krishan Lal Gupta, Vivekanand Jha
Use of active forms of vitamin D is advocated in CKD patients for treatment of mineral bone disease because of the presumption that native forms of vitamin D would not undergo significant activation to calcitriol, the most active biological form of vitamin D. We present secondary analysis looking at bone turnover in subjects who completed the randomized, double blind, placebo controlled trial investigating the effect of cholecalciferol supplementation on vascular function in non-diabetic CKD stage G3-4 and vitamin D ≤20ng/ml [CTRI/2013/05/003648]...
October 17, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29034731/effects-of-omega-3-fatty-acids-on-bone-turnover-markers-in-postmenopausal-women-systematic-review-and-meta-analysis
#4
D Shen, X Zhang, Z Li, H Bai, L Chen
AIM: There is conflicting evidence regarding the effects of omega-3 fatty acids on bone turnover markers in postmenopausal women. Thus, we systematically reviewed the efficacy of omega-3 fatty acids by conducting a meta-analysis of available randomized controlled trials. METHODS: PubMed, Embase, Cochrane Library and Scopus were searched in December 2016. The standardized mean difference (SMD) or weighted mean difference (WMD) and the corresponding 95% confidence intervals (CIs) were calculated using a fixed-effects model...
October 16, 2017: Climacteric: the Journal of the International Menopause Society
https://www.readbyqxmd.com/read/29032370/an-immunofluorescence-assay-for-extracellular-matrix-components-highlights-the-role-of-epithelial-cells-in-producing-a-stable-fibrillar-extracellular-matrix
#5
Omar S Qureshi, Hélène Bon, Breda Twomey, Gill Holdsworth, Kirsty Ford, Marianne Bergin, Linghong Huang, Mariusz Muzylak, Louise J Healy, Vanessa Hurdowar, Timothy S Johnson
Activated fibroblasts are considered major drivers of fibrotic disease progression through the production of excessive extracellular matrix (ECM) in response to signals from damaged epithelial and inflammatory cells. Nevertheless, epithelial cells are capable of expressing components of the ECM, cross-linking enzymes that increase its stability and are sensitive to factors involved in the early stages of fibrosis. We therefore wanted to test the hypothesis that epithelial cells can deposit ECM in response to stimulation in a comparable manner to fibroblasts...
October 15, 2017: Biology Open
https://www.readbyqxmd.com/read/29032110/use-of-donors-predisposed-by-corneal-collagen-cross-linking-in-penetrating-keratoplasty-for-treating-the-patients-with-keratoconus
#6
Ting Huang, Ruifen Ye, Chen Ouyang, Chao Hou, Yunwei Hu, Qianni Wu
PURPOSE: To investigate whether use of donors predisposed by corneal collagen cross-linking (CXL) reduced myopic refractive errors for keratoconic eyes after penetrating keratoplasty (PK). DESIGN: Randomized controlled trial. METHODS: One hundred sixteen eyes of 116 patients with keratoconus from Zhongshan Ophthalmic Center were enrolled. Using stratified block randomization, eligible eyes were assigned to CXL graft group (Group1) or conventional graft group (Group2)...
October 12, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29030341/macrophage-overexpression-of-matrix-metalloproteinase-9-in-aged-mice-improves-diastolic-physiology-and-cardiac-wound-healing-following-myocardial-infarction
#7
Cesar A Meschiari, Mira Jung, Rugmani Padmanabhan Iyer, Andriy Yabluchanskiy, Hiroe Toba, Michael R Garrett, Merry L Lindsey
Matrix metalloproteinase (MMP)-9 increases in the myocardium with advanced age and after MI. Because young transgenic (TG) mice overexpressing human MMP-9 only in macrophages mice show better outcomes post-MI, while aged TG mice show a worse aging phenotype, we wanted to evaluate the combination effect to see if the detrimental effect of aging counteracted the benefits of macrophage MMP-9 overexpression. We used 17-28 month old male and female C57BL/6J wild type (WT) mice and TG mice (n = 10-21/group) to evaluate the effects of aging superimposed on MI...
October 13, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29030302/mesenchymal-stem-cell-therapy-for-retro-corneal-membrane-a-clinical-challenge-in-full-thickness-transplantation-of-biosynthetic-corneal-equivalents
#8
Vijayalakshmi Rajendran, Magdalena Netuková, May Griffith, John V Forrester, Lucia Kuffová
Artificial corneas (keratoprostheses) and biosynthetic collagen-based corneal equivalents are surgical implants designed to ease the global burden of corneal blindness. However, keratoprostheses in many cases fail due to development of fibrous retro-corneal membranes (RCM). Fibrous membranes which develop in the anterior chamber after prosthesis implantation do so on a matrix of fibrin. This study investigated fibrin deposition and RCM formation after full-thickness collagen-based hydrogel implants and compared them with syngeneic and allogeneic corneal grafts in mice...
October 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29030187/not-only-tendons-the-other-architecture-of-collagen-fibrils
#9
REVIEW
Mario Raspanti, Marcella Reguzzoni, Marina Protasoni, Petra Basso
For many decades the fibrillar collagens have been the subject of a remarkable body of ultrastructural research. The vast majority of the studies, however, were carried out on tendon or on tendon-derived material. For many reasons this reflects an obvious choice but at the same time it also is an unfortunate circumstance, because this flooding of tendon-related data can easily encourage the false confidence that all connective tissues are similar. The reality is quite the opposite, and a different fibrillar structure has been long time observed on collagen fibrils from different tissues, the most notable example being offered by corneal fibrils...
October 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29024928/zinc-inhibits-collagenolysis-by-cathepsin-k-and-matrix-metalloproteinases-in-demineralized-dentin-matrix
#10
Pinar Altinci, Roda Seseogullari-Dirihan, Gulsen Can, David Pashley, Arzu Tezvergil-Mutluay
The enzymatic degradation of dentin organic matrix occurs via both the action of matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs). Zinc can prevent collagen hydrolysis by MMPs. However, its effect on the activity of dentin-bound CCs is not known. The aim of this study was to investigate the effect of zinc on matrix-bound cathepsin K and MMP activity in dentin. Completely demineralized dentin beams were divided into test groups (n = 9) and incubated at 37°C in an incubation media (1 mL) containing ZnCl2 of 0...
October 13, 2017: Caries Research
https://www.readbyqxmd.com/read/28987632/moderate-hyperhomocysteinemia-induced-by-short-term-dietary-methionine-overload-alters-bone-microarchitecture-and-collagen-features-during-growth
#11
Petar Milovanovic, Dragan Hrncic, Ksenija Radotic, Mira Stankovic, Dragosav Mutavdzic, Danijela Djonic, Aleksandra Rasic-Markovic, Dragan Djuric, Olivera Stanojlovic, Marija Djuric
AIMS: In general, hyperhomocysteinemia is increasingly appreciated as a risk factor for various diseases, including osteoporosis. However, its effects in non-adults remain largely unknown. Our aim was to determine whether dietary-caused increased homocysteine levels have deleterious effects on bone structure during growth. MAIN METHODS: We developed a model of moderate hyperhomocysteinemia caused by short-term methionine nutritional overload in growing rats. 30-days-old male Wistar albino rats were randomly assigned to either experimental group subject to a 30-days hypermethionine diet or control group...
October 4, 2017: Life Sciences
https://www.readbyqxmd.com/read/28985813/collagen-cross-linking-as-an-adjunct-for-repair-of-corneal-lacerations-a-cadaveric-study
#12
Timothy Ratzlaff, David Wei, Yao Wang, Mark Xu, Davin Johnson
OBJECTIVE: To determine the efficacy of collagen cross-linking (CXL) as an adjunct to suturing in the repair of corneal lacerations. METHODS: A cadaveric study was undertaken in which a linear 5 mm corneal laceration was created in the central cornea of 20 eyes. The eyes were then randomized to receive 1 (n = 8), 2 (n = 8), or 3 (n = 4) standard corneal sutures. The burst pressure of the wound was then measured. All eyes in the 1- and 2-suture group then underwent standard CXL, with burst pressure repeated afterward...
October 2017: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/28971683/hydroxylamine-chemical-digestion-for-insoluble-extracellular-matrix-characterization
#13
Alexander S Barrett, Matthew J Wither, Ryan C Hill, Monika Dzieciatkowska, Angelo D'Alessandro, Julie A Reisz, Kirk C Hansen
The extracellular matrix (ECM) is readily enriched by decellularizing tissues with non-denaturing detergents to solubilize and deplete the vast majority of cellular components. This approach has been used extensively to generate ECM scaffolds for regenerative medicine technologies and in 3D cell culture to model how the ECM contributes to disease progression. A highly enriched ECM fraction can then be generated using a strong chaotrope buffer that is compatible with downstream bottom-up proteomic analysis or 3D cell culture experiments after extensive dialysis...
October 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28971529/a-novel-protein-therapeutic-joint-retention-strategy-based-on-collagen-binding-avimers
#14
Joanne T Hulme, Warren N D'Souza, Helen J McBride, Bo-Rin Park Yoon, Angela M Willee, Amy Duguay, Melissa Thomas, Bin Fan, Maria Rosalyn Dayao, James B Rottman, Kim Merriam, Jiansong Xie, Richard Smith, Benjamin M Alba, Ryan B Case, Khue Dang, Anielka Montalvan, Natalia Grinberg, Hong Sun, Roy A Black, Christopher A Gabel, John E Sims, Kevin Moore, Alice Bakker, Peng Li
Designing drugs to treat diseases associated with articular joints, particularly those targeting chondrocytes, is challenging due to unique local environmental constraints including the avascular nature of cartilage, the absence of a closed joint compartment, and a highly cross-linked extracellular matrix. In an effort to address these challenges, we developed a novel strategy to prolong residence time of intra-articularly administered protein therapeutics. Avimer domains are naturally found in membrane polypeptides and mediate diverse protein-protein interactions...
October 3, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28970517/human-in-vitro-model-reveals-the-effects-of-collagen-cross-linking-on-keratoconus-pathogenesis
#15
Rabab Sharif, Jesper Hjortdal, Henrik Sejersen, Garett Frank, Dimitrios Karamichos
Keratoconus (KC) is a corneal thinning disorder that leads to severe vision impairment As opposed to corneal transplantation; corneal collagen crosslinking (CXL) is a relatively non-invasive procedure that leads to an increase in corneal stiffness. In order to evaluate the effect of CXL on human corneal stromal cells in vitro, we developed a 3-D in vitro CXL model, using primary Human corneal fibroblasts (HCFs) from healthy patients and Human Keratoconus fibroblasts (HKCs) from KC patients. Cells were plated on transwell polycarbonate membranes and stimulated by a stable vitamin C...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28966224/interaction-between-bone-and-glucose-metabolism-review
#16
Ippei Kanazawa
Accumulating evidence has shown that bone and glucose metabolism are closely associated with each other. Since the risk of osteoporotic fractures is increased in patients with diabetes mellitus (DM), osteoporosis is recently recognized as one of diabetic complications, called DM-induced bone fragility. Previous studies showed that collagen cross-links of advanced glycation end products (AGEs) and dysfunctions of osteoblast and osteocyte are involved in DM-induced bone fragility. Circulating levels of AGEs and homocysteine are increased in patients with DM, and they directly impair the functions of osteoblast and osteocyte, resulting in decreased bone formation and bone remodeling...
September 30, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28964571/fractures-in-patients-with-ckd-diagnosis-treatment-and-prevention-a-review-by-members-of-the-european-calcified-tissue-society-and-the-european-renal-association-of-nephrology-dialysis-and-transplantation
#17
REVIEW
Ana Pimentel, Pablo Ureña-Torres, M Carola Zillikens, Jordi Bover, Martine Cohen-Solal
Mineral and bone disease is omnipresent in patients with chronic kidney disease (CKD) and leads to a diverse range of clinical manifestations, including bone pain and fractures. The accumulation of traditional clinical risk factors, in addition to those related to CKD, enhances the risk of comorbidity and mortality. Despite significant advances in understanding bone disease in CKD, most clinical and biochemical targets used in clinical practice remain controversial, resulting in an undermanagement of bone fragility...
September 27, 2017: Kidney International
https://www.readbyqxmd.com/read/28963018/temporal-development-of-near-native-functional-properties-and-correlations-with-qmri-in-self-assembling-fibrocartilage-treated-with-exogenous-loxl2
#18
Pasha Hadidi, Derek D Cissell, Jerry C Hu, Kyriacos A Athanasiou
Advances in cartilage tissue engineering have led to constructs with mechanical integrity and biochemical composition increasingly resembling that of native tissues. In particular, collagen cross-linking with lysyl oxidase has been used to significantly enhance the mechanical properties of engineered neotissues. In this study, development of collagen cross-links over time, and correlations with tensile properties, were examined in self-assembling neotissues. Additionally, quantitative MRI metrics were examined in relation to construct mechanical properties as well as pyridinoline cross-link content and other engineered tissue components...
September 26, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28960383/agonist-induced-platelet-reactivity-correlates-with-bleeding-in-haemato-oncological-patients
#19
B Batman, E R van Bladel, M van Hamersveld, P C M Pasker-de Jong, S J A Korporaal, R T Urbanus, M Roest, L A Boven, R Fijnheer
BACKGROUND AND OBJECTIVE: Prophylactic platelet transfusions are administered to prevent bleeding in haemato-oncological patients. However, bleeding still occurs, despite these transfusions. This practice is costly and not without risk. Better predictors of bleeding are needed, and flow cytometric evaluation of platelet function might aid the clinician in identifying patients at risk of bleeding. This evaluation can be performed within the hour and is not hampered by low platelet count...
September 29, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28947902/demineralized-freeze-dried-bovine-cortical-bone-its-potential-for-guided-bone-regeneration-membrane
#20
David B Kamadjaja, Achmad Harijadi, Pratiwi Soesilawati, Eny Wahyuni, Nurul Maulidah, Akhsanal Fauzi, Fika Rah Ayu, Roberto Simanjuntak, R Soesanto, Djodi Asmara, Andra Rizqiawan, Peter Agus, Coen Pramono
BACKGROUND: Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) whose manufacturing process usually required chemical cross-linking to prolong its biodegradation. However, cross-linking of collagen fibrils was associated with poorer tissue integration and delayed vascular invasion. OBJECTIVE: This study evaluated the potential of bovine cortical bone collagen membrane for GBR by evaluating its antigenicity potential, cytotoxicity, immune and tissue response, and biodegradation behaviors...
2017: International Journal of Dentistry
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