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https://www.readbyqxmd.com/read/28204754/hydrolysis-of-phytate-to-its-lower-esters-can-influence-the-growth-performance-and-nutrient-utilization-of-broilers-with-regular-or-super-doses-of-phytase
#1
L A Beeson, C L Walk, M R Bedford, O A Olukosi
No abstract text is available yet for this article.
February 16, 2017: Poultry Science
https://www.readbyqxmd.com/read/28186144/engineering-the-residual-side-chains-of-hap-phytases-to-improve-their-pepsin-resistance-and-catalytic-efficiency
#2
Canfang Niu, Peilong Yang, Huiying Luo, Huoqing Huang, Yaru Wang, Bin Yao
Strong resistance to proteolytic attack is important for feed enzymes. Here, we selected three predicted pepsin cleavage sites, L99, L162, and E230 (numbering from the initiator M of premature proteins), in pepsin-sensitive HAP phytases YkAPPA from Yersinia kristensenii and YeAPPA from Y. enterocolitica, which corresponded to L99, V162, and D230 in pepsin-resistant YrAPPA from Y. rohdei. We constructed mutants with different side chain structures at these sites using site-directed mutagenesis and produced all enzymes in Escherichia coli for catalytic and biochemical characterization...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28174082/%C3%AE-propeller-phytases-diversity-catalytic-attributes-current-developments-and-potential-biotechnological-applications
#3
REVIEW
Vinod Kumar, Ajar Nath Yadav, Priyanka Verma, Punesh Sangwan, Abhishake Saxena, Krishan Kumar, Bijender Singh
Phytases are phosphatases which stepwise remove phosphates from phytic acid or its salts. β-Propeller phytase (BPPhy) belongs to a special class of microbial phytases that is regarded as most diverse, isolated and characterized from different microbes, mainly from Bacillus spp. BPPhy class is unique for its Ca(2+)-dependent catalytic activity, strict substrate specificity, active at neutral to alkaline pH and high thermostability. Numerous sequence and structure based studies have revealed unique attributes and catalytic properties of this class, as compared to other classes of phytases...
February 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28131338/response-based-selection-of-barley-cultivars-and-legume-species-for-complementarity-root-morphology-and-exudation-in-relation-to-nutrient-source
#4
Courtney D Giles, Lawrie K Brown, Michael O Adu, Malika M Mezeli, Graeme A Sandral, Richard J Simpson, Renate Wendler, Charles A Shand, Daniel Menezes-Blackburn, Tegan Darch, Marc I Stutter, David G Lumsdon, Hao Zhang, Martin S A Blackwell, Catherine Wearing, Patricia Cooper, Philip M Haygarth, Timothy S George
Phosphorus (P) and nitrogen (N) use efficiency may be improved through increased biodiversity in agroecosystems. Phenotypic variation in plants' response to nutrient deficiency may influence positive complementarity in intercropping systems. A multicomponent screening approach was used to assess the influence of P supply and N source on the phenotypic plasticity of nutrient foraging traits in barley (H. vulgare L.) and legume species. Root morphology and exudation were determined in six plant nutrient treatments...
February 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28126602/the-effect-of-feed-supplementation-with-zinc-chelate-and-zinc-sulphate-on-selected-humoral-and-cell-mediated-immune-parameters-and-cytokine-concentration-in-broiler-chickens
#5
Łukasz Jarosz, Agnieszka Marek, Zbigniew Grądzki, Małgorzata Kwiecień, Marcin Kalinowski
The ability of poultry to withstand infectious disease caused by bacteria, viruses or protozoa depends upon the integrity of the immune system. Zinc is important for proper functioning of heterophils, mononuclear phagocytes and T lymphocytes. Numerous data indicate that the demand for zinc in poultry is not met in Poland due to its low content in feeds of vegetable origin. The aim of the study was to determine the effect of supplementation of inorganic (ZnSO4 and ZnSO4(+) phytase enzyme), and organic forms of zinc (Zn with glycine and Zn with glycine and phytase enzyme) on selected parameters of the cellular and humoral immune response in broiler chickens by evaluating the percentage of CD3(+)CD4(+), CD3(+)CD8(+), CD25(+), MHC Class II, and BU-1(+) lymphocytes, the phagocytic activity of monocytes and heterophils, and the concentration of IL-2, IL-10 and TNF-α in the peripheral blood...
September 14, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/28111437/dose-dependent-effects-of-a-microbial-phytase-on-phosphorus-digestibility-of-common-feedstuffs-in-pigs
#6
Ferdinando N Almeida, Mercedes Vazquez-Anon, Jeffery Escobar
Objective: To evaluate concentrations of a microbial phytase (Cibenza® Phytaverse®, Novus International, USA) on standardized total tract digestibility (STTD) of P in canola meal (CM), corn, corn-derived distiller's dried grains with solubles (DDGS), rice bran (RB), sorghum, soybean meal (SBM), sunflower meal (SFM), and wheat. Methods: Two cohorts of 36 pigs each (initial BW = 78.5 ± 3.7 kg) were randomly assigned to 2 rooms, each housing 36 pigs, and then allotted to 6 diets with 6 replicates per diet in a randomized complete block design...
January 13, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28076998/phosphate-phytate-and-phytases-in-plants-from-fundamental-knowledge-gained-in-arabidopsis-to-potential-biotechnological-applications-in-wheat
#7
David Secco, Nadia Bouain, Aida Rouached, Chanakan Prom-U-Thai, Moez Hanin, Ajay K Pandey, Hatem Rouached
Phosphorus (P) is an essential macronutrient for all living organisms. In plants, P is taken up from the rhizosphere by the roots mainly as inorganic phosphate (Pi), which is required in large and sufficient quantities to maximize crop yields. In today's agricultural society, crop yield is mostly ensured by the excessive use of Pi fertilizers, a costly practice neither eco-friendly or sustainable. Therefore, generating plants with improved P use efficiency (PUE) is of major interest. Among the various strategies employed to date, attempts to engineer genetically modified crops with improved capacity to utilize phytate (PA), the largest soil P form and unfortunately not taken up by plants, remains a key challenge...
January 12, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/27990026/assessment-of-bioavailable-organic-phosphorus-in-tropical-forest-soils-by-organic-acid-extraction-and-phosphatase-hydrolysis
#8
Tegan Darch, Martin S A Blackwell, David Chadwick, Philip M Haygarth, Jane M B Hawkins, Benjamin L Turner
Soil organic phosphorus contributes to the nutrition of tropical trees, but is not accounted for in standard soil phosphorus tests. Plants and microbes can release organic anions to solubilize organic phosphorus from soil surfaces, and synthesize phosphatases to release inorganic phosphate from the solubilized compounds. We developed a procedure to estimate bioavailable organic phosphorus in tropical forest soils by simulating the secretion processes of organic acids and phosphatases. Five lowland tropical forest soils with contrasting properties (pH 4...
December 15, 2016: Geoderma
https://www.readbyqxmd.com/read/27938322/protein-consensus-based-surface-engineering-procos-a-computer-assisted-method-for-directed-protein-evolution
#9
Amol V Shivange, Hans Wolfgang Hoeffken, Stefan Haefner, Ulrich Schwaneberg
Protein consensus-based surface engineering (ProCoS) is a simple and efficient method for directed protein evolution combining computational analysis and molecular biology tools to engineer protein surfaces. ProCoS is based on the hypothesis that conserved residues originated from a common ancestor and that these residues are crucial for the function of a protein, whereas highly variable regions (situated on the surface of a protein) can be targeted for surface engineering to maximize performance. ProCoS comprises four main steps: (i) identification of conserved and highly variable regions; (ii) protein sequence design by substituting residues in the highly variable regions, and gene synthesis; (iii) in vitro DNA recombination of synthetic genes; and (iv) screening for active variants...
December 1, 2016: BioTechniques
https://www.readbyqxmd.com/read/27900695/function-based-metagenomic-library-screening-and-heterologous-expression-strategy-for-genes-encoding-phosphatase-activity
#10
Genis A Castillo Villamizar, Heiko Nacke, Rolf Daniel
The release of phosphate from inorganic and organic phosphorus compounds can be mediated enzymatically. Phosphate-releasing enzymes, comprising acid and alkaline phosphatases, are recognized as useful biocatalysts in applications such as plant and animal nutrition, bioremediation and diagnostic analysis. Metagenomic approaches provide access to novel phosphatase-encoding genes. Here, we describe a function-based screening approach for rapid identification of genes conferring phosphatase activity from small-insert and large-insert metagenomic libraries derived from various environments...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27894040/plausible-exploitation-of-jatropha-de-oiled-seed-cake-for-lipase-and-phytase-production-and-simultaneous-detoxification-by-candida-parapsilosis-isolated-from-poultry-garbage
#11
Balakrishna Kannoju, Swaruparani Ganapathiwar, Hanumalal Nunavath, Bindu Sunkar, Bhima Bhukya
Jatropha de-oiled seed cake was explored to utilize as a basic nutrient source for Candida parapsilosis, isolated from poultry garbage and selected based on the production of lipase and phytase enzymes under submerged fermentation. At optimized parameters under solid-state fermentation, lipase and phytase activities were recorded as 1056.66±2.92 and 833±2.5U/g of substrate (U/g), respectively. Besides enzyme production, complete elimination of phorbol esters and significant phytate reduction from 6.51±0...
February 2017: Bioresource Technology
https://www.readbyqxmd.com/read/27872714/effects-of-dietary-supplementation-of-organic-acids-and-phytase-on-performance-and-intestinal-histomorphology-of-broilers
#12
Nasibeh Mohammadagheri, Ramin Najafi, Gholamreza Najafi
The present experiment was conducted to evaluate the effects of organic acids and phytase enzyme supplementation on performance and intestinal histomorphology of broilers. The experiment was done in a factorial arrangement 2 × 2 × 2 based on completely randomized design with eight treatments, five replicates with 12 chicks in each until 42 days of age. Diets included natural vinegar (0 and 2%), citric acid (CA; 0.00 and 1.00%) and phytase enzyme (PHY; 0.00 and 500 FTU phytase per kg of feed). One bird from each treatment replicate was randomly selected and slaughtered to evaluate the small intestinal morphology on 42 days of age...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27868294/effect-of-dietary-calcium-level-and-source-on-mineral-utilisation-by-piglets-fed-diets-containing-exogenous-phytase
#13
P Schlegel, A Gutzwiller
Calcium and phosphorus are essential minerals, closely linked in digestive processes and metabolism. With widespread use of low P diets containing exogenous phytase, the optimal dietary Ca level was verified. The 40-day study evaluated the effects of Ca level (4, 7 and 10 g/kg diet) and Ca source (Ca from CaCO3 and from Lithothamnium calcareum) on mineral utilisation in 72 piglets (7.9 ± 1.0 kg BW) fed an exogenous phytase containing diet with 2.9 g digestible P/kg. Measured parameters were growth performance, stomach mineral solubility, bone breaking strength and urinary, serum and bone mineral concentration...
November 21, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27825490/phosphate-dissolving-fungi-mechanism-and-application-in-alleviation-of-salt-stress-in-wheat
#14
Sunita Gaind
The present investigation reveals the solubilization efficiency of tri-calcium phosphate (TCP), Udaipur rock phosphate (URP), aluminium phosphate (AP) and ferric phosphate (FP) by Aspergillus niger (ITCC 6719) and Trichoderma harzianum (ITCC 6721) as function of carbon concentrations. Increasing glucose concentration from 1 to 7% in the growth medium, though improved the phosphorus (P) solubilization significantly but each fungal strain preferred different optimum carbon concentrations for mediating solubilization of different P sources...
December 2016: Microbiological Research
https://www.readbyqxmd.com/read/27812526/evaluation-and-selection-of-bacillus-species-based-on-enzyme-production-antimicrobial-activity-and-biofilm-synthesis-as-direct-fed-microbial-candidates-for-poultry
#15
Juan D Latorre, Xochitl Hernandez-Velasco, Ross E Wolfenden, Jose L Vicente, Amanda D Wolfenden, Anita Menconi, Lisa R Bielke, Billy M Hargis, Guillermo Tellez
Social concern about misuse of antibiotics as growth promoters (AGP) and generation of multidrug-resistant bacteria have restricted the dietary inclusion of antibiotics in livestock feed in several countries. Direct-fed microbials (DFM) are one of the multiple alternatives commonly evaluated as substitutes of AGP. Sporeformer bacteria from the genus Bacillus have been extensively investigated because of their extraordinary properties to form highly resistant endospores, produce antimicrobial compounds, and synthesize different exogenous enzymes...
2016: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/27796873/phytase-production-and-development-of-an-ideal-dephytinization-process-for-amelioration-of-food-nutrition-using-microbial-phytases
#16
Jinender Jain, Bijender Singh
Development of an ideal process for reduction of food phytates using microbial phytases is a demanding task by all food and feed industries all over the world. Phytase production by Bacillus subtilis subsp. subtilis JJBS250 isolated from soil sample was optimized in submerged fermentation using statistical tools. Among all the culture variables tested, sucrose, sodium phytate and Tween-80 were identified as the most significant variables using the Placket-Burman design. Further optimization of these variables resulted in a 6...
October 29, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27790831/strain-improvement-of-pichia-kudriavzevii-ty13-for-raised-phytase-production-and-reduced-phosphate-repression
#17
Linnea Qvirist, Egor Vorontsov, Jenny Veide Vilg, Thomas Andlid
In this work, we present the development and characterization of a strain of Pichia kudriavzevii (TY1322), with highly improved phytate-degrading capacity. The mutant strain TY1322 shows a biomass-specific phytate degradation of 1.26 mmol g(-1)  h(-1) after 8 h of cultivation in a high-phosphate medium, which is about 8 times higher compared with the wild-type strain. Strain TY1322 was able to grow at low pH (pH 2), at high temperature (46°C) and in the presence of ox bile (2% w/v), indicating this strain's ability to survive passage through the gastrointestinal tract...
October 28, 2016: Microbial Biotechnology
https://www.readbyqxmd.com/read/27789748/use-of-zn-concentration-in-the-gastrointestinal-tract-as-a-measure-of-phytate-susceptibility-to-the-effect-of-phytase-supplementation-in-broilers
#18
N K Morgan, D V Scholey, E J Burton
Zinc (Zn) is the most vulnerable cation to complexation with phytate. An experiment was conducted to evaluate the potential of measurements of Zn concentration in the gastrointestinal tract as a marker to assess the anti-nutritional impact of phytate and susceptibility of phytate to phytase in broilers. Ross 308 broilers (n = 180) were fed one of 5 experimental diets with differing phytase activity levels, analyzed at 605, 1150, 1804, 3954, and 5925 U/kg. Broiler performance and Zn concentration, pH, and amount of phytate hydrolyzed in the gizzard, duodenum, and ileum were analyzed at d 21 post hatch...
October 27, 2016: Poultry Science
https://www.readbyqxmd.com/read/27760159/variations-of-phosphorous-accessibility-causing-changes-in-microbiome-functions-in-the-gastrointestinal-tract-of-chickens
#19
Bruno Tilocca, Maren Witzig, Markus Rodehutscord, Jana Seifert
The chicken gastrointestinal tract (GIT) harbours a complex microbial community, involved in several physiological processes such as host immunomodulation and feed digestion. For the first time, the present study analysed dietary effects on the protein inventory of the microbiome in crop and ceca of broilers. We performed quantitative label-free metaproteomics by using 1-D-gel electrophoresis coupled with LC-MS/MS to identify the structural and functional changes triggered by diets supplied with varying amount of mineral phosphorous (P) and microbial phytase (MP)...
2016: PloS One
https://www.readbyqxmd.com/read/27759065/effect-of-phytase-on-zinc-absorption-from-a-millet-based-porridge-fed-to-young-burkinabe-children
#20
M Brnić, R F Hurrell, L T Songré-Ouattara, B Diawara, A Kalmogho-Zan, C Tapsoba, C Zeder, R Wegmüller
BACKGROUND/OBJECTIVES: Fortifying cereal staples with zinc is a strategy for increasing zinc intake in young children in developing countries. However, phytic acid (PA) naturally present in cereals strongly decreases zinc absorption. A stable-isotope zinc absorption study was conducted in young children to investigate the ability of the PA-degrading enzyme phytase to improve zinc absorption, when added to a cereal porridge immediately before consumption. SUBJECTS/METHODS: Fractional absorption of zinc (FAZ) was estimated in 35 young healthy Burkinabe children using the double-isotopic tracer ratio method with (67)Zn as oral tracer and (70)Zn as intravenous tracer, in a crossover design...
January 2017: European Journal of Clinical Nutrition
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