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Xianwei Zhang, Zicong Li, Huaqiang Yang, Dewu Liu, Gengyuan Cai, Guoling Li, Jianxin Mo, Dehua Wang, Cuili Zhong, Haoqiang Wang, Yue Sun, Junsong Shi, Enqin Zheng, Fanming Meng, Mao Zhang, Xiaoyan He, Rong Zhou, Jian Zhang, Miaorong Huang, Ran Zhang, Ning Li, Mingzhe Fan, Jinzeng Yang, Zhenfang Wu
In pig production, inefficient feed digestion causes excessive nutrients such as phosphorus and nitrogen to be released to the environment. To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. All the transgenic enzymes were successfully expressed, and the digestion of non-starch polysaccharides (NSPs) and phytate in the feedstuff was enhanced...
May 22, 2018: ELife
Seung-Min Hong, Hyuk-Joon Kwon, Se-Joon Park, Won-Jin Seong, Ilhwan Kim, Jae-Hong Kim
The yeast strain SJP-SNU was investigated as a probiotic and was characterized with respect to growth temperature, bile salt resistance, hydrogen sulfide reducing activity, intestinal survival ability and chicken embryo pathogenicity. In addition, we determined the complete genomic and mitochondrial sequences of SJP-SNU and conducted comparative genomics analyses. SJP-SNU grew rapidly at 37 °C and formed colonies on MacConkey agar containing bile salt. SJP-SNU reduced hydrogen sulfide produced by Salmonella serotype Enteritidis and, after being fed to 4-week-old chickens, could be isolated from cecal feces...
May 17, 2018: AMB Express
Sophie A Lee, Devanaboyina Nagalakshmi, Mantina V L N Raju, Savaram V Rama Rao, Michael R Bedford
A total of 2,376 one-day-old Ross broiler chickens were used to investigate the effect of myo-inositol (MYO) and phytase supplementation on performance and bone mineralization variables in broilers fed diets formulated to have varying concentrations of available phosphorus (P). The trial was designed as a 2 × 2 × 3 factorial; with and without phytase superdosing (0 or 1,500 FTU/kg), MYO (0 or 3 g/kg), and dietary P (low, moderate or high). At 21 d, dietary phytase and MYO had no consistent benefit on bone mineralization variables...
September 2017: Animal nutrition
Tiago T Dos Santos, Helen V Masey O'Neill, Gemma González-Ortiz, Daniel Camacho-Fernández, Carlos López-Coello
The interaction of xylanase, protease and superdosing (1,500 FTU/kg) phytase in a 2 × 2 × 2 factorial arrangement was studied in broilers fed sorghum-based diets. A total of 2,800 one-day-old unsexed Ross 308 chicks were housed in 56 pens with 50 birds per pen, with or without inclusion of xylanase, protease and phytase, totaling 8 treatments and 7 replicates per treatment. Body weight (BW) and feed intake (FI) were measured at 21 and 42 days of age, and mortality corrected feed conversion ratio (FCR) was calculated for each period and cumulatively...
June 2017: Animal nutrition
Alexander Eck, Matthias Schmidt, Stefanie Hamer, Anna Joelle Ruff, Jan Förster, Ulrich Schwaneberg, Lars M Blank, Wolfgang Wiechert, Marco Oldiges
Background: Expanding the application of technical enzymes, e.g., in industry and agriculture, commands the acceleration and cost-reduction of bioprocess development. Microplates and shake flasks are massively employed during screenings and early phases of bioprocess development, although major drawbacks such as low oxygen transfer rates are well documented. In recent years, miniaturization and parallelization of stirred and shaken bioreactor concepts have led to the development of novel microbioreactor concepts...
2018: Fungal Biology and Biotechnology
Connie Gallardo, Julio Cezar Dadalt, Messias Alves Trindade Neto
The study was conducted to determine the effects of multicarbohydrase (MC) preparation (700 U α-galactosidase, 2,200 U galactomannanase, 3,000 U xylanase, and 22,000 U β-glucanase per kg of diet) and phytase (Phy, 500 FTU per kg of diet) supplementation on the nutritive value of wheat bran (WB) in broiler chicks. Trial 1 determined retention of nutrients and apparent metabolizable energy corrected by nitrogen (AMEn). One reference diet (RD) protein-free (85% corn based) was fortified to determine the WB nutrient retention coefficient...
May 8, 2018: Journal of Animal Science
Viviane Cristina Toreti Theodoropoulos, Mariana Amarante Turatti, Ralf Greiner, Gabriela Alves Macedo, Juliana Azevedo Lima Pallone
The objective of this study was to evaluate in vitro bioaccessibility of calcium (Ca), iron (Fe) and zinc (Zn) in soy drink after phytase treatment and correlate it with the content of myo-inositol phosphates. Samples of commercial soy drink products and one sample produced in the laboratory by maceration were evaluated. Phytase was applied using 300 U per liter in 60 min considering the phosphate release. The content of myo-inositol tris-, tetrakis-, pentakis and hexakisphosphate was not observed after phytase treatment...
June 2018: Food Research International
Alessandra De Cesare, Federica Palma, Alex Lucchi, Frederique Pasquali, Gerardo Manfreda
In the last few years metagenomic and 16S rRNA sequencing have completly changed the microbiological investigations of food products. In this preliminary study, the microbiological profile of chicken carcasses collected from animals fed with different diets were tested by using shotgun metagenomic sequencing. A total of 15 carcasses have been collected at the slaughetrhouse at the end of the refrigeration tunnel from chickens reared for 35 days and fed with a control diet (n=5), a diet supplemented with 1500 FTU/kg of commercial phytase (n=5) and a diet supplemented with 1500 FTU/kg of commercial phytase and 3g/kg of inositol (n=5)...
March 31, 2018: Italian Journal of Food Safety
Alex Sander Rodrigues Cangussu, Deborah Aires Almeida, Raimundo Wagner de Souza Aguiar, Sidnei Emilio Bordignon-Junior, Kelvinson Fernandes Viana, Luiz Carlos Bertucci Barbosa, Edson Wagner da Silva Cangussu, Igor Viana Brandi, Augustus Caeser Franke Portella, Gil Rodrigues Dos Santos, Eliane Macedo Sobrinho, William James Nogueira Lima
Phytase plays a prominent role in monogastric animal nutrition due to its ability to improve phytic acid digestion in the gastrointestinal tract, releasing phosphorus and other micronutrients that are important for animal development. Moreover, phytase decreases the amounts of phytic acid and phosphate excreted in feces. Bioinformatics approaches can contribute to the understanding of the catalytic structure of phytase. Analysis of the catalytic structure can reveal enzymatic stability and the polarization and hydrophobicity of amino acids...
2018: Enzyme Research
Y She, J C Sparks, H H Stein
An experiment was conducted to test the hypothesis that inclusion of increasing concentrations of an Escherichia coli (E. coli) phytase to a corn-soybean meal (SBM) diet results in improved digestibility of DM, GE, CP, NDF, ADF, macro minerals, micro minerals, and AA. Twenty four growing barrows (initial BW: 37.0 ± 1.4 kg) were equipped with a T-cannula in the distal ileum and placed individually in metabolism crates, and allotted to a 2-period switch-back design with 6 diets and 4 replicate pigs per diet in each period...
April 24, 2018: Journal of Animal Science
Bahareh Pakbaten, Reza Majidzadeh Heravi, Hassan Kermanshahi, Mohammad-Hadi Sekhavati, Ali Javadmanesh, Masoud Mohammadi Ziarat
Probiotics are beneficial microorganisms and have long been used in food production as well as health promotion products. Bioengineered probiotics are used to express and transfer native or recombinant molecules to the mucosal surface of the digestive tract to improve feed efficiency and promote health. Lactococcus lactis is a potential probiotic candidate to produce useful biological proteins. The aim of this investigation was to develop a recombinant Lactococcus lactis with the potential of producing phytase...
April 21, 2018: Probiotics and Antimicrobial Proteins
Bijender Singh, K K Sharma, Amit Kumari, Anil Kumar, S K Gakhar
A thermostable and protease-resistant HAP-phytase of Sporotrichum thermophile was over-expressed in Pichia pastoris ×33. Purified recombinant phytase displayed all its biochemical properties similar to wild type. Molecular modeling and docking of phytase with various substrates showed differential binding patterns with GoldScore values ranging from 40.61 to 79.78. Docking with different substrates revealed strong binding affinity with ATP and phytic acid, while the lowest with AMP and phosphoenol pyruvate...
April 17, 2018: International Journal of Biological Macromolecules
Łukasz Jarosz, Agnieszka Marek, Zbigniew Grądzki, Ewa Laskowska, Małgorzata Kwiecień
The aim of the study was to determine how inorganic and organic forms of zinc affect the concentrations of C-reactive protein (CRP), serum amyloid A (SAA), alpha-1-acid glycoprotein (α-1-AGP), haptoglobin (Hp), and transferrin (TRF) in the blood and liver tissue of 450 1-day-old Ross 308 chicken. Four experimental groups received one the following: inorganic zinc (ZnSO4 ), a zinc phytase enzyme supplement (ZnSO4 -F), organic zinc in combination with glycine (Zn-Gly), or organic zinc supplemented with phytase (Zn-Gly-F)...
April 19, 2018: Biological Trace Element Research
Grzegorz Kłosowski, Dawid Mikulski, Oliwia Jankowiak
One of the key steps in the production of phytases of microbial origin is selection of culture parameters, followed by isolation of the enzyme and evaluation of its catalytic activity. It was found that conditions for S. cerevisiae yeast culture, strain Finarome, giving the reduction in phytic acid concentration of more than 98% within 24 h of incubation were as follows: pH 5.5, 32 °C, continuous stirring at 80 rpm, the use of mannose as a carbon source and aspartic acid as a source of nitrogen. The highest catalytic activity of the isolated phytase was observed at 37 °C, pH 4...
April 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Maciej M Misiura, João A N Filipe, Carrie L Walk, Ilias Kyriazakis
Ca digestibility and utilisation in growing pigs are not well understood, and are usually neglected in diet formulation. This has implications not only for the accurate determination of its requirements but also for its interactions with other nutrients. A systematic review and meta-analysis (meta-regression) of published trials was carried out to quantify factors affecting Ca absorption and utilisation, and to derive an estimate of Ca endogenous excretion. The analysis was carried out on the data from forty studies, corresponding to 201 treatments performed on 1204 pigs...
April 3, 2018: British Journal of Nutrition
C L Walk, V Pirgozliev, K Juntunen, M Paloheimo, D R Ledoux
Three experiments were conducted to evaluate eight neutral and six acid proteases on growth performance and apparent ileal amino acid digestibility (AID) of poults (Experiment 1) or chicks (Experiments 2 and 3). Two basal diets were formulated: a nutrient adequate positive control (PC), which met or exceeded the nutrient requirements for poults (Experiment 1) or chicks (Experiments 2 and 3) and a negative control (NC) formulated to achieve 85% (Experiments 1 and 2) or 80% (Experiments 3) of the requirement for protein and amino acids...
March 27, 2018: Poultry Science
L H Vallejo, G Buendía R, M M Y Elghandour, D Menezes-Blackburn, R Geiner, A Z M Salem
BACKGROUND: The effect of phytase supplementation to a high sorghum grain diet on sheep voluntary feed intake, apparent nutrient digestibility, ruminal fermentation, phosphorus (P) excretion and blood serum P concentration was evaluated. RESULT: Phytase supplementation significantly decreased the fecal P excretion (P = 0.003) representing a 26% decrease in relation to the phytase free diet. Dry matter intake, nutrient digestibility, ruminal butyrate and serum P were not (P > 0...
March 31, 2018: Journal of the Science of Food and Agriculture
Amit Ranjan, Narottam Prasad Sahu, Ashutosh Dharmendra Deo, H Sanath Kumar, Sarvendra Kumar, Kamal Kant Jain
A 60-day feeding trial was conducted to study the effect of exogenous enzymes (xylanase and phytase) supplementation in the non-fermented and fermented de-oiled rice bran (DORB)-based diet of Labeo rohita. Four test diets (T1-DORB-based diet, T2-fermented DORB-based diet, T3-phytase and xylanase supplemented DORB-based diet, and T4-phytase and xylanase supplemented fermented DORB-based diet) were formulated and fed to the respective groups. Test diets T3 and T4 were supplemented with 0.01% xylanase (16,000 U kg-1 ) and 0...
March 29, 2018: Fish Physiology and Biochemistry
Youbin Kong, Xihuan Li, Bing Wang, Wenlong Li, Hui Du, Caiying Zhang
Induction and secretion of acid phosphatases (APases) is considered to be an important strategy for improving plant growth under conditions of low inorganic phosphate (Pi). Purple acid phosphatases (PAPs), are an important class of plant APases that could be secreted into the rhizosphere to utilize organic phosphorus (Po) for plant growth and development. To date, only a few members of the PAP family have been identified in soybean. In this paper, we identified a secreted PAP in soybean, GmPAP14, and investigated its role in utilizing external phytate, the main form of organic phosphorus in the soil...
2018: Frontiers in Plant Science
Sib Sankar Giri, Shib Sankar Sen, Subrata Saha, Venkatachalam Sukumaran, Se Chang Park
This study aimed to isolate potential probiotic lactic acid bacteria from a traditional rice-based fermented beverage "bhaati jaanr" and to evaluate their role during preparation of the beverage. Among various isolates, Lactobacillus plantarum strain L7 exhibited satisfactory in vitro probiotic characteristics such as acid resistance and bile tolerance, cell surface hydrophobicity, auto-aggregation, antibiotic susceptibility, and antimicrobial activities. Therefore, performance of L7 as a starter culture in rice fermentation was determined during a 6-day rice fermentation study...
2018: Frontiers in Microbiology
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