keyword
MENU ▼
Read by QxMD icon Read
search

phytase

keyword
https://www.readbyqxmd.com/read/29145111/a-novel-protein-tyrosine-phosphatase-like-phytase-from-lactobacillus-fermentum-nkn51-cloning-characterization-and-application-in-mineral-release-for-food-technology-applications
#1
Rekha Sharma, Piyush Kumar, Vandana Kaushal, Rahul Das, Naveen Kumar Navani
A novel protein tyrosine phosphatase like phytase (PTPLP), designated as PhyLf from probiotic bacterium Lactobacillus fermentum NKN51 was identified, cloned, expressed and characterized. The recombinant PhyLf showed specific activity of 174.5 U/mg. PhyLf exhibited strict specificity towards phytate and optimum temperature at 60 °C, pH 5.0 and ionic strength of 100 mM. Km and Kcat of PhyLf for phytate were 0.773 mM and 84.31 s(-1), respectively. PhyLf exhibited high resistance against oxidative inactivation...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29108630/improved-thermostability-and-enzyme-activity-of-a-recombinant-phya-mutant-phytase-from-aspergillus-niger-n25-by-directed-evolution-and-site-directed-mutagenesis
#2
Zizhong Tang, Weiqiong Jin, Rong Sun, Yan Liao, Tianrun Zhen, Hui Chen, Qi Wu, Lin Gou, Chenlei Li
We previously constructed three recombinant phyA mutant strains (PP-NP(m)-8, PP-NP(ep)-6A and I44E/T252R-PhyA), showing improved catalytic efficiency or thermostability of Aspergillus niger N25 phytase, by error-prone PCR or site-directed mutagenesis. In this study, directed evolution and site-directed mutagenesis were further applied to improve the modified phytase properties. After one-round error-prone PCR for phytase gene of PP-NP(ep)-6A, a single transformant, T195L/Q368E/F376Y, was obtained with the significant improvements in catalytic efficiency and thermostability...
January 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29095154/crystallization-and-x-ray-diffraction-analysis-of-native-and-selenomethionine-substituted-phyh-di-from-bacillus-sp-hjb17
#3
Fang Lu, Bei Zhang, Yong Liu, Ying Song, Gangxing Guo, Duo Feng, Huoqing Huang, Peilong Yang, Wei Gao, Sujuan Guo, Bin Yao
Phytases are phosphatases that hydrolyze phytates to less phosphorylated myo-inositol derivatives and inorganic phosphate. β-Propeller phytases, which are very diverse phytases with improved thermostability that are active at neutral and alkaline pH and have absolute substrate specificity, are ideal substitutes for other commercial phytases. PhyH-DI, a β-propeller phytase from Bacillus sp. HJB17, was found to act synergistically with other single-domain phytases and can increase their efficiency in the hydrolysis of phytate...
November 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/29077965/phosphorus-bioavailability-in-increased-protein-reduced-fiber-canola-meal-conventional-canola-meal-and-soybean-meal-fed-to-crossbred-chicks
#4
C D Hanna, C K Foran, P L Utterback, H H Stein, C M Parsons
Three experiments were conducted to evaluate 3 increased-protein, reduced-fiber canola meals (CM) (CMA, CMB, and Test CM), 2 conventional CM (CCM), and 2 soybean meals (SBM). For determination of P bioavailability in CM and SBM, a P-deficient cornstarch-dextrose-SBM basal diet was fed as Diet 1. The latter basal diet was then supplemented with 0.05 and 0.10% P from KH2PO4 or 12.5 and 25% of a CM or SBM. In addition, the effect of phytase enzyme on bioavailability of the P in CMA, Test CM, and one of the CCM was determined using P-deficient 45% CM-cornstarch-dextrose diets (0...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29077957/effects-of-a-high-level-of-phytase-on-broiler-performance-bone-ash-phosphorus-utilization-and-phytate-dephosphorylation-to-inositol
#5
A E Gautier, C L Walk, R N Dilger
An experiment was conducted to evaluate how the addition of microbial phytase influenced growth performance, bone mineralization, tissue P content, apparent digestibility and retention, and inositol phosphate (IP) concentrations in broilers fed diets with varying mineral matrices from 2 to 23 d of age. At 2 d of age, chicks were randomly allotted to receive 1 of 6 experimental diets arranged as a 3 × 2 factorial of mineral matrix [control diet with 1.0% Ca and 0.5% non-phytate phosphorus (NPP); mineral matrix 1 with 0...
October 25, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053834/effect-of-feed-supplementation-with-zinc-glycine-chelate-and-zinc-sulfate-on-cytokine-and-immunoglobulin-gene-expression-profiles-in-chicken-intestinal-tissue
#6
L Jarosz, A Marek, Z Gradzki, M Kwiecien, B Zylinska, B Kaczmarek
The aim of the study was to evaluate the effect of inorganic and organic forms of Zn on the expression of cytokines (IL-2, TNF-α, IFN-γ, IL-12, IL-17, IL-4, IL-10, and TGF-β) and immunoglobulins (IgA and IgG) in the tissues of the small intestine (jejunum and ileum) of broiler chickens. In the experiment, 90 broiler chickens were divided into 4 experimental groups and a control group, with 18 birds each. The birds received Zn supplements in inorganic form with and without phytase (ZnSO4 and ZnSO4 + F), and in organic form with glycine, with and without phytase (Zn-Gly and Zn-Gly + F)...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053809/comparative-effects-of-two-multi-enzyme-combinations-and-a-bacillus-probiotic-on-growth-performance-digestibility-of-energy-and-nutrients-disappearance-of-non-starch-polysaccharides-and-gut-microflora-in-broiler-chickens
#7
A L Wealleans, M C Walsh, L F Romero, V Ravindran
The efficacy of two exogenous enzyme combinations and a multi-strain Bacillus probiotic (DFM) on the growth performance, nutrient digestibility, disappearance of non-starch polysaccharides (NSP) and gut microbial composition was investigated in broilers. One-day old Ross 308 chicks were assigned to 36 pens with 22 birds/pen and 6 pens/treatment (Experiment 1) or 36 cages with 8 birds/cage and 6 cages/treatment (Experiment 2). Treatment additives were added to nutritionally complete corn/soy based starter (d 1 to 21) and finisher (d 22 to 42) diets...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29052415/activity-of-escherichia-coli-aspergillus-niger-and-rye-phytase-toward-partially-phosphorylated-myo-inositol-phosphates
#8
Ralf Greiner
Kinetic parameters for the dephosphorylation of sodium phytate and a series of partially phosphorylated myo-inositol phosphates were determined at pH 3.0 and pH 5.0 for three phytase preparations (Aspergillus niger, Escherichia coli, rye). The enzymes showed lower affinity and turnover numbers at pH 3 compared to pH 5 toward all myo-inositol phosphates included in the study. The number and distribution of phosphate groups on the myo-inositol ring affected the kinetic parameters. Representatives of the individual phytate dephosphorylation pathways were identified as the best substrates of the phytases...
October 31, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29046263/improvement-of-extracellular-bacterial-protein-production-in-pichia-pastoris-by-co-expression-of-endoplasmic-reticulum-residing-groel-groes
#9
Pijug Summpunn, Juntratip Jomrit, Watanalai Panbangred
Pichia pastoris is an established host system for heterologous protein expression. However, the potential productivity of this system can be limited. In this study, the Escherichia coli chaperones (GroES-GroEL) were expressed from the PGAP promoter and targeted to the secretory pathway through the endoplasmic reticulum (ER). The ability of the ER targeted chaperones to improve production of bacterial protein in P. pastoris was evaluated. The chaperones tagged with α-factor secretion- and ER retention-signal sequences were co-expressed with either extracellularly secreted phytase or intracellular d-phenylglycine aminotransferase (D-PhgAT) enzymes...
October 15, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29027600/improved-hydrolase-activity-in-barley-and-reduced-malting-time-by-adding-phytase-as-an-activator-during-malting-steeping
#10
Ran Qiu, Jian Lu
OBJECTIVES: Exogenous phytase improved the activity of hydrolases to decrease the malting time. RESULTS: Treatment with phytase during barley steeping increased activity of hydrolases (α-/β-amylase, proteinase, β-glucanase and xylanase) in green malt. Maximal activity was observed for α-/β-amylase, β-glucanase and xylanase with 0.8 U phytase/g and proteinase with 1.2 U phytase/g. Phytase promoted acrospire growth of green malt and reduced malting process with 0...
October 12, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29024064/physiological-and-health-related-response-of-broiler-chickens-fed-diets-containing-raw-full-fat-soya-bean-meal-supplemented-with-microbial-protease
#11
M M Erdaw, R A Perez-Maldonado, P A Iji
A 2 × 3 factorial study (protease: 0 or 1,5000 PROT/kg and raw full-fat soya bean meal [RSBM] replacing the commercial SBM at 0, 45 and 75 g/kg of diet) was conducted to examine the performance of broilers. Phytase (2000 FYT/kg) was uniformly added to each diet, each also replicated six times, with eight birds per replicate. Birds were raised in climate-controlled rooms using sawdust as the bedding material and offered starter, grower and finisher diets. Feed intake (FI) and body weight gain (BWG) were reduced (p < ...
October 10, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29017237/biochemical-characterization-of-a-psychrophilic-phytase-from-an-artificially-cultivable-morel-morchella-importuna
#12
Hao Tan, Jie Tang, Xiaolin Li, Tianhai Liu, Renyun Miao, Zhongqian Huang, Yong Wang, Bingcheng Gan, Weihong Peng
Psychrophilic phytases suitable for aquaculture are rare. In this study, a phytase of histidine acidic phosphatase (HAP) family was identified in Morchella importuna, a psychrophilic mushroom. The phytase showed 38% identity with Aspergillus niger PhyB, which was the closest hit. The M. importuna phytase was overexpressed in Pichia pastoris, purified and characterized. The phytase had an optimum temperature at 25°C, which is the lowest among all the known phytases to our knowledge. The optimum pH (6.5) is higher than most of the known HAP phytases, which is fit for the weak acidic condition in fish gut...
October 11, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28991999/effect-of-combined-xylanase-and-phytase-on-growth-performance-apparent-total-tract-digestibility-and-carcass-characteristics-in-growing-pigs-fed-corn-based-diets-containing-high-fiber-coproducts
#13
Y D Jang, P Wilcock, R D Boyd, M D Lindemann
Phytate has been shown to be an antinutrient, and the feeding of high levels of phytase can break down phytate to improve nutrient utilization and pig performance. Dietary xylanase targets arabinoxylan breakdown, thereby improving energy utilization in pigs. However, the effects of simultaneous supplementation have not been clearly determined. Crossbred pigs ( = 45; mean initial weight, 26.4 ± 0.2 kg) were allotted to 1 of 9 treatments to evaluate the effects of both xylanase (endo-1,4-β xylanase [EC 3.2...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28990379/effects-of-neutral-phytase-on-growth-performance-and-phosphorus-utilization-in-crucian-carp-carassius-auratus
#14
Xin-Zheng Nie, Sha Chen, Xiao-Xu Zhang, Bin-Yang Dai, Li-Chun Qian
A feeding trial was conducted for nine weeks to investigate the effects of partially replacing Ca(H2PO4)2 with neutral phytase on the growth performance, phosphorus utilization, nutrient digestibility, serum biochemical parameters, bone and carcass mineral composition, and digestive-enzyme-specific activity in crucian carp (Carassius auratus). The diets prepared with 0.8%, 0%, and 1.8% Ca(H2PO4)2 (1%=1 g/100 g) supplements were regarded as the P1E0, negative control (NC), and positive control (PC) groups, respectively; the other three experimental diets were prepared with the addition of 200, 300, and 500 U/kg of neutral phytase, respectively, based on the P1E0 group...
2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28955309/a-novel-vector-for-construction-of-markerless-multicopy-overexpression-transformants-in-pichia-pastoris
#15
Ding Li, Bo Zhang, Shuting Li, Jie Zhou, Hui Cao, Yan Huang, Zhongli Cui
Pichia pastoris is widely used as a platform for heterologous protein expression because of its high volumetric productivity. Multicopy integration of the target gene is commonly used to improve the production of the target protein. Cre/lox recombination system is a powerful tool for the marker rescue during multiple integrations with one selection marker. Here we reported a novel expression vector based on the Cre/lox recombination system for multiple integrations of target gene to construct multicopy expression strain of P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28938791/effects-of-a-high-dose-of-microbial-phytase-and-myo-inositol-supplementation-on-growth-performance-tibia-mineralization-nutrient-digestibility-litter-moisture-content-and-foot-problems-in-broiler-chickens-fed-phosphorus-deficient-diets
#16
D Farhadi, A Karimi, Gh Sadeghi, J Rostamzadeh, M R Bedford
A total of 660 one-day-old Ross 308 broiler chicks were randomly distributed into eleven dietary treatments. Treatments included a maize-soybean meal-based diet with recommended calcium (Ca) and non-phytate phosphorus (nPP) (positive control; PC), an nPP-deficient diet (negative control; NC), NC diets supplemented with different levels of phytase (0, 500, 1,000, 2,000, 3,000, 4,000, 5,000, and 6,000 FTU/kg), a NC diet plus 0.15% myo-inositol, and a NC diet with reduced Ca level (Ca to nPP ratio same as PC)...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938789/impacts-of-dietary-calcium-phytate-and-phytase-on-inositol-hexakisphosphate-degradation-and-inositol-phosphate-release-in-different-segments-of-digestive-tract-of-broilers
#17
W Li, R Angel, S-W Kim, K Brady, S Yu, P W Plumstead
A total of 720 straight-run Heritage 56 M × fast feathering Cobb 500F broiler chickens was fed from 11 to 13 d of age to determine the impacts of dietary calcium (Ca), phytate phosphorus (PP), and phytase concentrations on inositol phosphate (IP3-6) profile in different digestive tract (GI) segments. The experiment was a 2 × 2 × 3 randomized block design with 2 Ca (0.7 and 1.0%) and 2 PP (0.23 and 0.34%) concentrations and 3 doses of Buttiauxella sp. phytase (0, 500, and 1,000 FTU/kg). The experiment was replicated in time (block) with 3 replicates per treatment (Trt) of 10 birds per block...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28936766/use-of-lactobacilli-in-cereal-legume-fermentation-and-as-potential-probiotics-towards-phytate-hydrolysis
#18
Girish K Amritha, G Venkateswaran
Phytate is a potent inhibitor of mineral absorption in humans occurring in plant-based food. Application of lactobacilli that produce phytate-degrading enzymes (phytases) to reduce phytate is an interesting yet a not much explored sector of research. Therefore, phytate dephosphorylation by Lactobacillus plantarum MTCC 1325 was evaluated. Cells at stationary phase showed phytase activity which was maximal at 24 h of growth. Glucose concentration and the type of phosphorous source in the media modulated the enzyme activity...
September 21, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28926677/ca-2-influxes-and-transmembrane-transport-are-essential-for-phytic-acid-degradation-in-mung-bean-sprouts
#19
Ting Zhou, Pei Wang, Runqiang Yang, Xinyue Wang, Zhenxin Gu
BACKGROUND: Phytic acid is considered as an antinutrient. Ca(2+) addition during germination has been proved to be an effective method for reducing phytic acid content in seeds. In this study, mung bean sprouts were treated with LaCl3 (La), verapamil (VP), ruthenium red (RR) and CaCl2 to explore the effect of Ca(2+) influxes on phytic acid degradation. RESULTS: 6 mM CaCl2 significantly improved extracellular and intracellular calcium precipitates and calcium content, elevated phytase and acid phosphatase activity and further enhanced phytic acid degradation...
September 19, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28925862/biofactories-for-the-production-of-recombinant-phytases-and-its-application-in-animal-feed-industry
#20
Thais P P de Souza, Resyla M da Mariano, Mariana S Vieira, Suliana F V Andrade, Renato R Godoi, Ana Flavia A Gonçalves, Luciana P Naves, William J N Lima, Daniel B Gonçalves, Mariana Campos da Paz, Alexsandro S Galdino
BACKGROUND: Phytases are enzymes capable of degrading phytic acid and are used in animal feed supplementation in order to improve digestibility through the release of minerals such as phosphorus. Recent inventions show interest in production and optimization of recombinant phytases with biochemical and physicochemical characteristics promising for animal feed industry. METHODS: This review article is focused on relevant patents of the promising phytases, as well as the commonly used expression systems for its production, and also the tools currently used to generate new phytases...
September 15, 2017: Recent Patents on Biotechnology
keyword
keyword
102603
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"