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bone histochemistry

Suchismita Daw, Ritam Chatterjee, Aditya Law, Sujata Law
Hematological disorders like myelodysplastic syndrome (MDS) may arise due to cumulative dysregulation of various signalling pathways controlling proliferation, differentiation, maturation and apoptosis of bone marrow cells. This devastating bone marrow condition can be due to consequential abnormalities in haematopoiesis as well as its supportive microenvironment. Although mutations related to JAK/STAT pathway are common in myeloproliferative neoplasms, further studies are required to fully explore the myelodysplastic scenario regarding the concerned pathway...
October 7, 2016: Chemico-biological Interactions
Hiromi Hongo, Muneteru Sasaki, Sachio Kobayashi, Tomoka Hasegawa, Tomomaya Yamamoto, Kanako Tsuboi, Erika Tsuchiya, Tomoya Nagai, Naznin Khadiza, Miki Abe, Ai Kudo, Kimimitsu Oda, Paulo Henrique Luiz de Freitas, Minqi Li, Hisayoshi Yurimoto, Norio Amizuka
Minodronate is highlighted for its marked and sustained effects on osteoporotic bones. To determine the duration of minodronate's effects, we have assessed the localization of the drug in mouse bones through isotope microscopy, after labeling it with a stable nitrogen isotope ([(15)N]-minodronate). In addition, minodronate-treated bones were assessed by histochemistry and transmission electron microscopy (TEM). Eight-week-old male ICR mice received [(15)N]-minodronate (1 mg/kg) intravenously and were sacrificed after 3 hr, 24 hr, 1 week, and 1 month...
October 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
Corine Martineau, Ouafa Najyb, Céline Signor, Éric Rassart, Robert Moreau
BACKGROUND: Apolipoprotein D (ApoD) is a member of the lipocalin family known to transport small hydrophobic ligands. A major site of ApoD expression in mice is the central nervous system where evidence suggests that it plays a protective role. Gene expression of ApoD was reported in bone-forming osteoblasts but its impact on bone metabolism remains undocumented. METHODS: We compared basic bone parameters of ApoD(-/-) (null) and transgenic (tg) mice to wild-type (wt) littermates through microCT and histochemistry, as well as ApoD expression and secretion in osteoblasts under various culture conditions through real-time PCR and immunoblotting...
September 2016: Metabolism: Clinical and Experimental
Arpita A Biswas, David J Goldhamer
The skeletal muscle niche is complex and heterogeneous. Over the past few decades, various groups have reported the existence of multiple adult stem cell populations within this environment. Techniques commonly used to identify and assess the differentiation capacities of these cellular fractions, oftentimes rare populations, include the use of lineage tracers, immunofluorescence and histochemistry, flow cytometry, gene expression assays, and phenotypic analysis in culture or in vivo. In 2012, our lab identified and characterized a skeletal-muscle resident Tie2+ progenitor that exhibits adipogenic, chondrogenic, and osteogenic differentiation potentials (Wosczyna et al...
2016: Methods in Molecular Biology
N M Gaifullin, A S Karyagina, A V Gromov, A A Terpilovskiy, D A Malanin, M V Demeshchenko, V V Novochadov
Experiments were carried out on 22 albino male Wistar rats to study the morphological peculiarities of osseointegration of titanium grafts with bioactive surface stimulated additionally with bone plastic material "Gamalant-paste-FORTE Plus" containing recombinant human bone morphogenetic protein-2 (rhBMP-2). In 9 rats the implants were placed into femoral bones after local treatment of bone canal with rhBMP-2-containing material. Another 9 animals were implanted but received no treatment, 4 rats formed the group of intact control...
2016: Morfologi︠a︡
Andiara De Rossi, Sandra Yasuyo Fukada, Moara De Rossi, Raquel Assed Bezerra da Silva, Alexandra Mussolino Queiroz, Paulo Nelson-Filho, Léa Assed Bezerra da Silva
INTRODUCTION: Although studies have recently shown that osteocytes embedded in mineralized bone matrix play an important role in bone diseases, the participation of cementocytes in apical periodontitis has not been evaluated. The aim of the present study was to evaluate the possible involvement of cementocytes in the development of apical periodontitis. METHODS: Apical periodontitis was experimentally induced in the lower first molars of wild-type mice by pulp exposure to the oral environment...
August 2016: Journal of Endodontics
Xiaofei Zhou, Jonathan Pace, Emily Filichia, Tao Lv, Brandon Davis, Barry Hoffer, Warren Selman, Yu Luo
OBJECTIVE: To determine the influence of the sonic hedgehog (shh) pathway and its receptor smoothened (smo), on the survival and functionality of dopaminergic (DA) neurons. BACKGROUND: During early development, shh induces the differentiation of DA neurons. However, it is unknown whether shh signaling is required in the maturation or maintenance of DA neurons during later development and adulthood due to the lethality of traditional shh knockout models. METHODS: We utilized the cre-loxP system to achieve late developmental stage and cell type-specific deletion of the shh receptor, smo, in DA neurons by crossing DATcre (dopamine transporter) mice with Smo(loxP/loxP) mice...
September 2016: Experimental Neurology
Marjolein M J Caron, Pieter J Emans, Andy Cremers, Don A M Surtel, Lodewijk W van Rhijn, Tim J M Welting
Heterotopic ossification is the abnormal formation of bone in soft tissues and is a frequent complication of hip replacement surgery. Heterotopic ossifications are described to develop via endochondral ossification and standard treatment is administration of indomethacin. It is currently unknown how indomethacin influences heterotopic ossification on a molecular level, therefore we aimed to determine whether indomethacin might influence heterotopic ossification via impairing the chondrogenic phase of endochondral ossification...
June 8, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
A Carbone, M Valente, L Annacontini, S Castellani, S Di Gioia, D Parisi, M Rucci, G Belgiovine, C Colombo, A Di Benedetto, G Mori, L Lo Muzio, A Maiorella, A Portincasa, M Conese
The potential of adipose-derived mesenchymal stromal (stem) cells (ADSCs) to differentiate into either osteoblasts or chondrocytes is controversial. In this study we investigated the multicapacity potential of ADSCs to differentiate towards adipocyte, osteoblast, and chondrocyte lineages when cells are seeded onto plastic in comparison with incubation with conditioned media (CM) obtained from differentiated cell types.ADSCs, obtained from liposuctions, were characterized for mesenchymal and hematopoietic markers by cytofluorimetry...
January 2016: Journal of Biological Regulators and Homeostatic Agents
Okan W Bastian, Leo Koenderman, Jacqueline Alblas, Luke P H Leenen, Taco J Blokhuis
The role of inflammatory cells in bone regeneration remains unclear. We hypothesize that leukocytes contribute to fracture healing by rapidly synthesizing an "emergency extracellular matrix (ECM)" before stromal cells infiltrate the fracture hematoma (FH) and synthesize the eventual collagenous bone tissue. 53 human FHs were isolated at different time points after injury, ranging from day 0 until day 23 after trauma and stained using (immuno)histochemistry. FHs isolated within 48 h after injury contained fibronectin(+) ECM, which increased over time...
March 2016: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Stefanie Inglis, David Christensen, David I Wilson, Janos M Kanczler, Richard O C Oreffo
BACKGROUND: A dynamic vasculature is a prerequisite for bone formation where the interaction of bone cells and endothelial cells is essential for both the development and the healing process of bone. Enhanced understanding of the specific mediators involved in bone cell and endothelial cell interactions offers new avenues for skeletal regenerative applications. This study has investigated the osteogenic and angiogenic potential of co-cultures of human foetal diaphyseal or epiphyseal cells with human umbilical vein endothelial cells (HUVEC) in the presence and absence of vascular endothelial growth factor (VEGF) supplementation...
2016: Stem Cell Research & Therapy
D Chappard, P Bizot, G Mabilleau, L Hubert
Several decades ago, aluminum encephalopathy associated with osteomalacia has been recognized as the major complication of chronic renal failure in dialyzed patients. Removal of aluminum from the dialysate has led to a disappearance of the disease. However, aluminum deposit occurs in the hydroxyapatite of the bone matrix in some clinical circumstances that are presented in this review. We have encountered aluminum in bone in patients with an increased intestinal permeability (coeliac disease), or in the case of prolonged administration of aluminum anti-acid drugs...
June 2016: Morphologie: Bulletin de L'Association des Anatomistes
J Pietzner, B M Merscher, P C Baer, R P Duecker, O Eickmeier, D Fußbroich, P Bader, D Del Turco, R Henschler, S Zielen, R Schubert
Ataxia telangiectasia is a genetic instability syndrome characterized by neurodegeneration, immunodeficiency, severe bronchial complications, hypersensitivity to radiotherapy and an elevated risk of malignancies. Repopulation with ATM-competent bone marrow-derived cells (BMDCs) significantly prolonged the lifespan and improved the phenotype of Atm-deficient mice. The aim of the present study was to promote BMDC engraftment after bone marrow transplantation using low-dose irradiation (IR) as a co-conditioning strategy...
April 2016: Bone Marrow Transplantation
Yi-Hua Bai, Hong-Ying Jiang, Xi-Yan Lian, Jian-Song Wang, Jia-Ping Wang
This study aims to explore the therapeutic effect of bone marrow mesenchymal stem cells on adriamycin nephrosis, and the potential mechanism. The rat experimental nephropathy model was established by unilateral nephrectomy combined repeated injecting adriamycin (ADR). Thirty adriamycin nephrosis rats were randomly divided into three groups, including ADR (n=10), MSCs transplantation through peripheral veins groups (M-V, n=10), and MSCs transplantation through right renal artery groups (M-A, n=10), and there was another normal control group (N, n=10)...
2015: International Journal of Clinical and Experimental Medicine
Hyo-Seol Lee, Mijeong Jeon, Mi Jung Jeon, Seong-Oh Kim, Seung-Hye Kim, Jae-Ho Lee, Jea-Ho Lee, Su-Jin Ahn, Yooseok Shin, Je Seon Song
The aim of this study is to compare the characteristics of stem cells derived from human exfoliated deciduous teeth (SHED) from cryopreserved intact deciduous teeth with those of fresh SHED. In total, 20 exfoliated deciduous teeth were randomly divided into a fresh group (f-SHED; n = 11) and cryopreserved group (c-SHED; n = 9; stored for 1-8 months). Following thawing and separation of the pulp, the SHED cells were cultured, and the characteristics as mesenchymal stem cells were investigated using proliferation assays, cell-cycle analysis, colony-forming unit-fibroblast (CFU-F) assays, and flow cytometry analyses...
December 2015: Cryobiology
Xiaofeng Xu, Wei Yang, Xuejin Zhang
OBJECTIVE To study the morphology, immunology, cyto- and molecular genetics of a patient with lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM), deletion of P53 gene and rearrangement of clonal T cell receptors-delta (TCR-delta) gene. METHODS The cell morphology and immunocytochemistry were analyzed by bone marrow testing and biopsy. Cellular immunology was analyzed by flow cytometry. Genetic analysis was carried out by chromosome karyotyping, fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR)...
October 2015: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Daniel Chappard, Guillaume Mabilleau, Didier Moukoko, Nicolas Henric, Vincent Steiger, Patrick Le Nay, Jean-Marie Frin, Charlotte De Bodman
Exostosis (or osteochondroma) is the most common benign bone tumor encountered in children and adults. Exostoses may occur as solitary or multiple tumors (in the autosomal syndromes of hereditary multiple exostoses). Exostoses are composed of cortical and medullary bone covered by an overlying hyaline cartilage cap. We have searched iron (Fe) and aluminum (Al) in the matrix of cortical and trabecular bone of 30 patients with exostosis. Al(3+) and Fe(3+) are two cations which can substitute calcium in the hydroxyapatite crystals of the bone matrix...
November 2015: Journal of Inorganic Biochemistry
Yubo Sun, Brian P Scannell, Patrick R Honeycutt, David R Mauerhan, James Norton H, Edward N Hanley
Osteoarthritis is a joint disease involved in articular cartilage, subchondral bone, meniscus and synovial membrane. This study sought to examine cartilage degeneration, subchondral bone mineral density (BMD) and meniscal mineral density (MD) in male Hartley, female Hartley and female strain 13 guinea pigs to determine the association of cartilage degeneration with subchondral BMD and meniscal MD. Cartilage degeneration, subchondral BMD and meniscal MD in 12 months old guinea pigs were examined with histochemistry, X-ray densitometry and calcium analysis...
2015: Open Rheumatology Journal
Jiang-tao Zhang, Mei Mei, Ce Jiang, Lei Feng, Jin Huang, Jia-jia Wang
PURPOSE: To explore the combined effects of recombinant human platelet-derived growth factor-BB(rhPDGF-BB)and recombinant human transforming growth factor-β1 (rhTGF-β1) on protein expression of FAK in osteolasts of the pressure side of orthodontic tooth in rats. METHODS: One hundred and sixty male Sprague-Dawley rats were randomly divided into 2 groups: experimental group(A) and control group(B). Each group was subdivided equally into 5 subgroups. Orthodontic tooth movement model was established...
August 2015: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
Zhangyuan Xiong, Rong Jiang, Xiangzhu Li, Yanna Liu, Fengjin Guo
Eukaryotic cells possess several mechanisms to adapt to endoplasmic reticulum (ER) stress and thereby survive. ER stress activates a set of signaling pathways collectively termed as the unfolded protein response (UPR). We previously reported that Bone morphogenetic protein 2 (BMP2) mediates mild ER stress and activates UPR signal molecules in chondrogenesis. The mammalian UPR protects the cell against the stress of misfolded proteins in the endoplasmic reticulum. Failure to adapt to ER stress causes the UPR to trigger apoptosis...
2015: International Journal of Molecular Sciences
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