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bone histochemistry

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https://www.readbyqxmd.com/read/29580573/-benign-and-malignant-giant-cell-rich-lesions-of-bone-pathological-diagnosis-with-special-emphasis-on-recent-immunohistochemistry-and-molecular-techniques
#1
Nicolas Macagno, Kevin Caselles, Sébastien Aubert, Virginie Audard, Anne Gomez-Brouchet, Christine Galant, Jean-Marc Guinebretière, Marie Karanian, Frédérique Larousserie, Béatrice Marie, Gonzague de Pinieux, Corinne Bouvier
The infiltration by numerous osteoclastic giant cells is a frequent finding in bone tumors and pseudo-tumors. Pathologists must integrate clinical and radiological data to achieve a correct diagnosis in bone pathology. Benign giant-cell rich lesions of bone encompass giant cell tumor of bone, aneurysmal bone cyst, chondroblastoma, brown tumor and fibrous cortical defect/non-ossifying fibroma. Amongst malignant neoplasms, variants of conventional osteosarcoma, undifferentiated pleomorphic sarcoma, leiomyosarcoma and bone metastasis must be discussed...
March 23, 2018: Annales de Pathologie
https://www.readbyqxmd.com/read/29545993/combined-staining-techniques-for-demonstration-of-staphylococcus-aureus-biofilm-in-routine-histopathology
#2
Louise Kruse Jensen, Nicole Lind Henriksen, Thomas Bjarnsholt, Kasper Nørskov Kragh, Henrik Elvang Jensen
Aim: Visualization of Staphylococcus aureus biofilm using histochemical staining and combined histochemistry (HC) and immunohistochemistry (IHC). Methods: The ability of S. aureus S54F9 to form biofilm was tested in vitro . Hereafter, infected bone tissue was collected from two different porcine models of osteomyelitis inoculated with S. aureus strain S54F9. The infection time was five and fifteen days, respectively. Twenty-five different histochemical staining protocols were tested in order to find the stains that could identify extracellular biofilm matrix...
2018: Journal of Bone and Joint Infection
https://www.readbyqxmd.com/read/29442525/comparative-analysis-of-mesenchymal-stem-cells-from-bone-marrow-adipose-tissue-and-dental-pulp-as-sources-of-cell-therapy-for-zone-of-stasis-burns
#3
Ozan Luay Abbas, Orhan Özatik, Zeynep Burçin Gönen, Serdal Öğüt, Fikriye Yasemin Özatik, Hasan Salkın, Ahmet Musmul
INTRODUCTION: The implantation of mesenchymal stem cells (MSCs) has been shown to exert benefits for the survival of the zone-of-stasis. However, the clinical experience indicates the importance of selecting the right source and type of stem cells. Therefore, we planned the current study to perform a quantitative comparison of MSCs isolated from three different sources to provide information useful in selection of the optimal source and to see whether critical mechanisms are conserved between different populations...
February 14, 2018: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/29422992/pyrroloquinoline-quinone-plays-an-important-role-in-rescuing-bmi-1-mice-induced-developmental-disorders-of-teeth-and-mandible-anti-oxidant-effect-of-pyrroloquinoline-quinone
#4
Yuanqing Huang, Ning Chen, Dengshun Miao
To investigate whether pyrroloquinoline quinine (PQQ) plays an important role in rescuing Bmi-1-/- mice induced developmental disorders of teeth and mandible by regulating oxidative stress. We fed Bmi-1-/- mice a diet supplemented with PQQ (BKO+PQQ), BKO mice with normal diet (BKO) and wild type mice with normal diet (WT) as controls. We compared the differences of dental, mandibular phenotype by means of X-ray photography, micro CT scanning and three-dimensional reconstruction, HE staining, histochemistry, immunoistohemistry, TUNEL staining, Western blot and Flow cytometry in three groups of animals...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29246648/human-mesenchymal-stromal-cell-therapy-for-damaged-cochlea-repair-in-nod-scid-mice-deafened-with-kanamycin
#5
Simone Bettini, Valeria Franceschini, Laura Astolfi, Edi Simoni, Benedetta Mazzanti, Alessandro Martini, Roberto P Revoltella
BACKGROUND: Kanamycin, mainly used in the treatment of drug-resistant-tuberculosis, is known to cause irreversible hearing loss. Using the xeno-transplant model, we compared both in vitro and in vivo characteristics of human mesenchymal stromal cells (MSCs) derived from adult tissues, bone marrow (BM-MSCs) and adipose tissue (ADSCs). These tissues were selected for their availability, in vitro multipotency and regenerative potential in vivo in kanamycin-deafened nod-scid mice. METHODS: MSCs were isolated from informed donors and expanded ex vivo...
February 2018: Cytotherapy
https://www.readbyqxmd.com/read/29151193/disturbances-of-diurnal-phase-markers-behavior-and-clock-genes-in-a-rat-model-of-depression-modulatory-effects-of-agomelatine-treatment
#6
K Højgaard, S L Christiansen, E V Bouzinova, O Wiborg
Major depressive disorder (MDD) is a growing problem worldwide. Though, the etiology remains unresolved, circadian rhythm disturbances are frequently observed in MDD and thus is speculated to play a key role herein. The present study focuses on circadian rhythm disturbances in the chronic mild stress (CMS) animal model of depression and examined whether the atypical antidepressant, agomelatine, which is mediating its action via melatonergic and serotonergic receptors, is capable of resynchronizing the perturbed rhythm...
November 19, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/29070109/-correlation-of-socs3-mrna-expression-level-with-clinical-characteristics-and-early-response-to-treatment-in-pediatric-acute-lymphoblastic-leukemia
#7
Zheng-Yu Wu, Ning-Ling Wang, Kang-Kang Liu, Jin-Hua Chu, Yan Wang, Lin-Hai Yang, Zhi-Wei Xie
Obejective: To investigate the expression level of suppressor of cytokine signaling SOCS3 mRNA in children's acute lymphoblastic leukemia(ALL); to analyze the relationship between the expresion level of SOCS3 mRNA and disease status and risks of ALL, and to explore the application of SOCS3 mRNA in evaluation of ALL disease status, risk and target therapy. METHODS: The expression levels of SOCS3 mRNA in bone marrow mononuclear cells from 45 cases of newly diagnosed ALL at initial diganosis and induction remission and 13 normal children as controls were detected by SYBR Green fluorescence quantitative PCR; the correlation of SOCS3 mRNA expression level with risk and clinical characteristics of ALL was analyzed by means of statistical method; the immunofluorescence histochemistry method and laser confocal microscopy were used to detect the sites and expression level of SOCS3 mRNA in bone marrow samples of ALL patients...
October 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29039747/development-of-useful-biomaterial-for-bone-tissue-engineering-by-incorporating-nano-copper-zinc-alloy-ncuzn-in-chitosan-gelatin-nano-hydroxyapatite-ch-g-nhap-scaffold
#8
Juan Carlos Forero, Eduardo Roa, Juan G Reyes, Cristian Acevedo, Nelson Osses
Ceramic and metallic nanoparticles can improve the mechanical and biological properties of polymeric scaffolds for bone tissue engineering (BTE). In this work, nanohydroxyapatite (nHAp) and nano-copper-zinc alloy (nCuZn) were added to a chitosan/gelatin (Ch/G) scaffold in order to investigate the effects on morphological, physical, and biocompatibility properties. Scaffolds were fabricated by a freeze-drying technique using different pre-freezing temperatures. Microstructure and morphology were studied by scanning electron microscopy (SEM), glass transition ( Tg ) was studied using differential scanning calorimetry (DSC), cell growth was estimated by MTT assay, and biocompatibility was examined in vitro and in vivo by histochemistry analyses...
October 17, 2017: Materials
https://www.readbyqxmd.com/read/29039565/hydrogen-sulfide-regulates-bone-remodeling-and-promotes-orthodontic-tooth-movement
#9
Haiya Pu, Yongmei Hua
Hydrogen sulfide (H2S) is a gas signaling molecule that has multiple influences on physiological and pathological processes in the mammalian body, including vasodilation, neurotransmission, inflammation, hypoxia sensing and bone remodeling. Our previous studies suggested that H2S might be involved in the periodontal tissue remodeling during the orthodontic tooth movement (OTM) via increasing periodontal ligament cell differentiation, tissue mineralization, bone formation and collagen synthesis. The aim of the present study was to investigate the effects of H2S on alveolar bone remodeling that is associated with tooth movement...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28975451/increased-mast-cell-degranulation-and-co-localization-of-mast-cells-with-the-nmda-receptor-1-during-healing-after-achilles-tendon-rupture
#10
Md Abdul Alim, Paul W Ackermann, Pernilla Eliasson, Parmis Blomgran, Per Kristiansson, Gunnar Pejler, Magnus Peterson
The role of inflammation and the mechanism of tendon healing after rupture has historically been a matter of controversy. The purpose of the present study is to investigate the role of mast cells and their relation to the NMDA receptor-1 (a glutamate receptor) during healing after Achilles tendon rupture. Eight female Sprague Dawley rats had their right Achilles tendon transected. Three weeks after rupture, histological quantification of mast cell numbers and their state of degranulation was assessed by histochemistry...
December 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28720305/ecto-5-nucleotidase-cd73-regulates-bone-formation-and-remodeling-during-intramembranous-bone-repair-in-aging-mice
#11
Vivian Bradaschia-Correa, Anne M Josephson, Alexander J Egol, Matthew M Mizrahi, Kevin Leclerc, Jason Huo, Bruce N Cronstein, Philipp Leucht
Ecto-5'-nucleotidase (CD73) generates adenosine, an osteoblast activator and key regulator of skeletal growth. It is unknown, however, if CD73 regulates osteogenic differentiation during fracture healing in adulthood, and in particular how CD73 activity regulates intramembranous bone repair in the elderly. Monocortical tibial defects were created in 46-52-week-old wild type (WT) and CD73 knock-out mice (CD73(-/-)) mice. Injury repair was analyzed at post-operative days 5, 7, 14 and 21 by micro-computed tomography (micro-CT), histomorphometry, proliferating cell nuclear antigen (PCNA) immunostaining, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) histochemistry...
October 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28688581/persistent-and-inducible-neogenesis-repopulates-progenitor-renin-lineage-cells-in-the-kidney
#12
Linda Hickmann, Anne Steglich, Michael Gerlach, Moath Al-Mekhlafi, Jan Sradnick, Peter Lachmann, Maria Luisa S Sequeira-Lopez, R Ariel Gomez, Bernd Hohenstein, Christian Hugo, Vladimir T Todorov
Renin lineage cells (RLCs) serve as a progenitor cell reservoir during nephrogenesis and after renal injury. The maintenance mechanisms of the RLC pool are still poorly understood. Since RLCs were also identified as a progenitor cell population in bone marrow we first considered that these may be their source in the kidney. However, transplantation experiments in adult mice demonstrated that bone marrow-derived cells do not give rise to RLCs in the kidney indicating their non-hematopoietic origin. Therefore we tested whether RLCs develop in the kidney through neogenesis (de novo differentiation) from cells that have never expressed renin before...
December 2017: Kidney International
https://www.readbyqxmd.com/read/28587628/osteoclast-profile-of-medication-related-osteonecrosis-of-the-jaw-secondary-to-bisphosphonate-therapy-a-comparison-with-osteoradionecrosis-and-osteomyelitis
#13
Christian Gross, Manuel Weber, Kay Creutzburg, Patrick Möbius, Raimund Preidl, Kerstin Amann, Falk Wehrhan
BACKGROUND: The medication-related osteonecrosis of the jaw secondary to bisphosphonate therapy [MRONJ (BP)] is characterized by non-healing exposed bone in the maxillofacial region. The pathogenesis of MRONJ (BP) is not fully understood. Giant, hypernucleated, inactive osteoclasts were found in MRONJ (BP) tissues, which indicated that accelerated cell-cell fusion might play a role. Dendritic cell-specific transmembrane protein (DC-STAMP) is associated with the cell-cell fusion of osteoclasts and precursor cells...
June 6, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28561533/emergence-of-bruton-s-tyrosine-kinase-negative-hodgkin-lymphoma-during-ibrutinib-treatment-of-chronic-lymphocytic-leukaemia
#14
Siobhan Glavey, John Quinn, Mary McCloy, Jeremy Sargent, Yvonne McCartney, Mark Catherwood, Teresa Marafioti, Mary Leader, Philip Murphy, Patrick Thornton
Chronic lymphocytic leukaemia (CLL) is a chronic B-cell lympho-proliferative disorder in which lymphomatous transformations occur in 5%-15% of patients. Histologically these cases resemble diffuse large B-cell lymphoma, or Richter's transformation, in over 80% of cases. Rare cases of transformation to Hodgkin lymphoma (HL) have been reported in the literature with an estimated prevalence of 0.4%. We report a case of a 67-year-old female with CLL treated with the novel Bruton's tyrosine kinase (Btk) inhibitor, ibrutinib, who subsequently presented with intractable fevers...
May 31, 2017: European Journal of Haematology
https://www.readbyqxmd.com/read/28506298/fecx-bar-a-novel-bmp15-mutation-responsible-for-prolificacy-and-female-sterility-in-tunisian-barbarine-sheep
#15
Narjess Lassoued, Zohra Benkhlil, Florent Woloszyn, Ahmed Rejeb, Mohamed Aouina, Mourad Rekik, Stephane Fabre, Sonia Bedhiaf-Romdhani
BACKGROUND: Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the "W" flock selected for improved prolificacy, we found prolific and infertile ewes with streaky ovaries. Blood genomic DNA was extracted from a subset of low-ovulating, prolific and infertile ewes of the "W" flock, and the entire coding sequences of GDF9 and BMP15 were sequenced...
May 15, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28464311/comparative-anatomy-of-the-dorsal-hump-in-mature-pacific-salmon
#16
COMPARATIVE STUDY
Kenta Susuki, Masatoshi Ban, Masaki Ichimura, Hideaki Kudo
Mature male Pacific salmon (Genus Oncorhynchus) demonstrate prominent morphological changes, such as the development of a dorsal hump. The degree of dorsal hump formation depends on the species in Pacific salmon. It is generally accepted that mature males of sockeye (O. nerka) and pink (O. gorbuscha) salmon develop most pronounced dorsal humps. The internal structure of the dorsal hump in pink salmon has been confirmed in detail. In this study, the dorsal hump morphologies were analyzed in four Pacific salmon species inhabiting Japan, masu (O...
July 2017: Journal of Morphology
https://www.readbyqxmd.com/read/28444165/proinflammatory-mediators-related-to-orthodontically-induced-periapical-root-resorption-in-rat-mandibular-molars
#17
Yoshiro Matsumoto, Siripen Sringkarnboriboon, Takashi Ono
Objectives: The early phase of orthodontic tooth movement involves acute inflammatory response that may induce bone resorption. The aim of this study was to localize and quantify cells in the periodontium expressing proinflammatory mediators during orthodontically induced periapical root resorption of the rat mandibular molars. Materials and methods: The levels of proinflammatory cytokines interleukin-1 (IL-1) α and β, tumor necrosis factor-α (TNF-α), inflammatory enzymes cyclooxygenase (COX) 1 and 2, and their product prostaglandin E2 (PGE2) in the root resorption site were compared to those in the corresponding area of the untreated periodontal ligament (PDL) of physiologically drifting teeth...
November 30, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28389071/high-mobility-group-box-1-is-associated-with-the-inflammatory-infiltration-and-alveolar-bone-destruction-in-rats-experimental-periapical-lesions
#18
Lingshuang Liu, Jing Deng, Qiuxia Ji, Bin Peng
INTRODUCTION: This study was conducted to observe the immunohistochemical localization of high-mobility group box 1 (HMGB1) and its receptor, Toll-like receptor 4 (TRL4), in the development of periapical lesions induced in rats. The possible role of these molecules in the pathogenesis of periapical lesions was also explored. METHODS: Periapical lesions developed within 35 days after mandibular first molar pulp exposure in Wistar rats. The animals were randomly killed at 0, 7, 14, 21, 28, and 35 days after pulp exposure...
April 4, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28372288/rat-bmsc-infusion-was-unable-to-ameliorate-inflammatory-injuries-in-tissues-of-mice-with-lps-induced-endotoxemia
#19
Xiaolin Xie, Hanhan Liu, Jinhua Wu, Yun Chen, Zhui Yu, Natalia De Isla, Xiaohua He, Yinping Li
BACKGROUND: Mesenchymal stem cells (MSC) have been shown to have potent immunoregulatory effects. They are able to mitigate inflammation in many contexts of immune disorders, including autoimmune diseases and graft-versus-host disease (GVHD). Endotoxemia can induce systematic inflammation in the body. In this study, we try to investigate whether MSC can attenuate inflammation in models of LPS-induced endotoxemia. METHODS: Bone marrow MSC (BMSC) were isolated and expanded from rats of 4~6-week age...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28096061/connexin37-deficiency-alters-organic-bone-matrix-cortical-bone-geometry-and-increases-wnt-%C3%AE-catenin-signaling
#20
Rafael Pacheco-Costa, Jay R Kadakia, Emily G Atkinson, Joseph M Wallace, Lilian I Plotkin, Rejane D Reginato
Deletion of connexin (Cx) 37 in mice leads to increased cancellous bone mass due to defective osteoclast differentiation. Paradoxically; however, Cx37-deficient mice exhibit reduced cortical thickness accompanied by higher bone strength, suggesting a contribution of Cx37 to bone matrix composition. Thus, we investigated whether global deletion of Cx37 alters the composition of organic bone extracellular matrix. Five-month-old Cx37-/- mice exhibited increased marrow cavity area, and periosteal and endocortical bone surface resulting in higher total area in tibia compared to Cx37+/+ control mice...
April 2017: Bone
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