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https://www.readbyqxmd.com/read/28633822/effects-of-pulsed-ultrasound-on-rheological-and-structural-properties-of-chicken-myofibrillar-protein
#1
Jing-Yu Wang, Yu-Ling Yang, Xiao-Zhi Tang, Wen-Xi Ni, Lei Zhou
The effects of pulsed ultrasound (PUS) (power: 240w) with varying time (0, 3, 6, 9, 12 and 15min) on rheological and structural properties of chicken myofibrillar protein (CMP) were examined. PUS treatment significantly caused a decrease in the viscosity coefficients (k) but an increase in the flow index (n) value of CMP solutions within short time (0-6min), while had no significant effect for longer time (9-15min). Besides, at 6min, the solubility and microstructure of CMP samples were optimum. The primary structure of CMP was not altered by PUS treatment...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28627282/gallic-acid-pamam-and-gallic-acid-phspholipid-conjugates-physicochemical-characterization-and-in-vivo-evaluation
#2
Ebtsam M Abdou, Marwa A Masoud
Gallic acid (GA) is a naturally occurring compound with valuable antioxidant activity. Its oral bioavailability is limited by its high metabolism and rapid clearance. In this paper, gallic acid (GA) was conjugated with two different materials, phosphatidylcholine (PC) and Polyamidoamine (PAMAM) dendrimer. The prepared conjugates were characterized by FTIR, DSC and SEM. Also, they were tested for drug content and in-vitro drug release. It was found that GA conjugation with both materials have significantly prolonged its release up to 12 hours...
June 19, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28624420/biophysical-characterization-and-thermal-stability-of-pneumococcal-histidine-triad-protein-d-phtd-in-the-presence-of-zinc-and-manganese
#3
Salvador F Ausar, Kavisha Jayasundara, Lamees Akawi, Cristopher Roque, Anthony Sheung, Jian Hu, Marina Kirkitadze, Nausheen Rahman
The Pneumococcal Histidine Triad Protein D (PhtD) is believed to play a central role in pneumococcal metal ion homeostasis and has been proposed as a promising vaccine candidate against pneumococcal disease. To investigate for potential stabilizers, a panel of physiologically relevant metals was screened using the thermal shift assay and it was found that only Zn(2+) and Mn(2+) were able to increase PhtD melting temperature. Differential Scanning Calorimetry (DSC) analysis revealed a sequential unfolding of PhtD and the presence of at least 3 independent folding domains that can be stabilized by Zn(2+) and Mn(2+)...
June 14, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28624082/the-influence-of-carrier-material-on-some-physical-and-structural-properties-of-carrot-juice-microcapsules
#4
Emilia Janiszewska-Turak, Nicolò Dellarosa, Urszula Tylewicz, Luca Laghi, Santina Romani, Marco Dalla Rosa, Dorota Witrowa-Rajchert
This study investigates the effect of carrier type on the physical and structural properties of microcapsules of pure carrot juice. Low-crystallised maltodextrin (MD), gum arabic (GA), mixtures of MD and GA (1:1; 2:1; 3:1) and whey protein isolate (WPI) were used as carriers. Microencapsulation was carried out in a spray-drier at inlet air temperature of 160°C. Powders were investigated for dry matter, water activity, diameter, carotene content and hygroscopicity. In addition, differential scanning calorimetry (DSC) and time domain nuclear magnetic resonance (TD-NMR) were applied to analyse microcapsules glass transition temperature (Tg)...
December 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28619638/development-and-characterization-of-cuttlefish-sepia-officinalis-skin-gelatin-protein-isolate-blend-films
#5
Hela Kchaou, Mourad Jridi, Ola Abdelhedi, Benbettaïeb Nasreddine, Thomas Karbowiak, Moncef Nasri, Frédéric Debeaufort
In the present study, cuttlefish skin gelatin (CSG) and protein isolate (CSPI) blend films were prepared and their physicochemical and antioxidant properties were characterized. CSG (4%, w/v) was blended with CSPI (3%, w/v) at different ratios and 15% of glycerol (w/w, plasticizer/polymer dry matter). Results showed that increasing of CSPI content induced lower lightness but higher redness, yellowness and color difference values compared to CSG film. In addition, CSPI film had the lowest tensile strength (TS) and elongation at break (EAB) values...
June 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28587076/structure-modification-of-an-active-azo-compound-as-a-route-to-new-antimicrobial-compounds
#6
Simona Concilio, Lucia Sessa, Anna Maria Petrone, Amalia Porta, Rosita Diana, Pio Iannelli, Stefano Piotto
Some novel (phenyl-diazenyl)phenols 3a-g were designed and synthesized to be evaluated for their antimicrobial activity. A previously synthesized molecule, active against bacteria and fungi, was used as lead for modifications and optimization of the structure, by introduction/removal or displacement of hydroxyl groups on the azobenzene rings. The aim of this work was to evaluate the consequent changes of the antimicrobial activity and to validate the hypothesis that, for these compounds, a plausible mechanism could involve an interaction with protein receptors, rather than an interaction with membrane...
May 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28585693/influences-of-soaking-temperature-and-storage-conditions-on-hardening-of-soybeans-glycine-max-and-red-kidney-beans-phaseolus-vulgaris
#7
Takako Koriyama, Yoko Sato, Kumiko Iijima, Midori Kasai
The influences of soaking treatment and storage conditions on the softening of cooked beans, namely, soybeans and red kidney beans, were investigated. It was revealed that the softening of fresh soybeans and fresh red kidney beans was suppressed during subsequent boiling after soaking treatment at 50 and 60 °C. Furthermore, in treated aged soybeans and red kidney beans that were subjected to storage at 30 °C/75% relative humidity for 6 mo and soaking treatment at 50 to 60 °C, the hardness during cooking was further amplified...
June 6, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28575991/electrospun-pcl-gelatin-composite-nanofiber-structures-for-effective-guided-bone-regeneration-membranes
#8
Ke Ren, Yi Wang, Tao Sun, Wen Yue, Hongyu Zhang
Guided bone regeneration (GBR) membranes have been proved of great benefit for bone tissue engineering due to the improvement of cell attachment and proliferation. To develop GBR membranes with better biocompatibility and more proper degradation ability, here we fabricated polycaprolactone (PCL, polymer)/gelatin (protein) hybrid nanofibrous GBR membranes via electrospinning, followed by crosslinking with genipin. Acetic acid (HAc) was utilized to resolve the phase separation of PCL and gelatin, therefore homogeneous PCL/gelatin hybrid nanofibers with different ratios were successfully prepared...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28561240/preparation-and-characterization-of-copolymeric-polymersomes-for-protein-delivery
#9
Alireza Nomani, Hamed Nosrati, Hamidreza Kheiri Manjili, Leila Khesalpour, Hossein Danafar
Biodegradable copolymeric polymersomes have been used for controlled drug delivery of proteins. These polymersomes important areas to overcome formulation associated problems of the proteins. The aim of this study was to develop polymersomes using biodegradable copolymers for delivery of bovine serum albumin (BSA) as a model protein. Encapsulated BSA by mPEG-PCL polymersomes led to formation of BSA-loaded mPEG-PCL polymersomes. The polymersomes synthesized with the protein-polymer ratio of 1:4 at 15 000 rpm gave maximum loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared polymersomes...
May 30, 2017: Drug Research
https://www.readbyqxmd.com/read/28556557/conjunctiva-derived-mesenchymal-stem-cell-cjmscs-as-a-potential-platform-for-differentiation-into-corneal-epithelial-cells-on-bioengineered-electrospun-scaffolds
#10
Fatemeh Soleimanifar, Yousef Mortazavi, Samad Nadri, Masoud Soleimani
With the aid of tissue engineering, the capability of nanofibrous scaffolds was examined for corneal epithelial differentiation of Conjunctiva mesenchymal stem cells (CJMSCs) as a new source. This study tried to provide a synthetic corneal sheet which could be applied in corneal regeneration. The hybrid arrangement of fine Silk fibers with the diameter of 93.65 nm and the polyurethane (PU) fibers with an average diameter of 575.5 makes a mat with sufficient mechanical properties and interconnected pores that could accelerate nutrient diffusion...
May 27, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28516782/exploring-site-specific-n-glycosylation-of-hek293-and-plant-produced-human-iga-isotypes
#11
Kathrin Göritzer, Daniel Maresch, Friedrich Altmann, Christian Obinger, Richard Strasser
The full potential of recombinant Immunoglobulin A as therapeutic antibody is not fully explored, owing to the fact that structure-function relationships of these extensively glycosylated proteins are not well understood. Here monomeric IgA1, IgA2m(1), and IgA2m(2) variants of the anti-HER2 antibody (IgG1) trastuzumab were expressed in glyco-engineered Nicotiana benthamiana plants and in human HEK293-6E cells. All three IgA isotypes were purified and subjected to biophysical and biochemical characterization...
May 26, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28516572/identification-of-ageing-biomarkers-in-human-dermis-biopsies-by-thermal-analysis-dsc-combined-with-fourier-transform-infrared-spectroscopy-ftir-atr
#12
R Tang, V Samouillan, J Dandurand, C Lacabanne, M-H Lacoste-Ferre, P Bogdanowicz, P Bianchi, A Villaret, F Nadal-Wollbold
BACKGROUND/PURPOSE: The purpose of this clinical study was to identify suitable biomarkers for a better understanding of the molecular and organizational changes in human dermis during intrinsic and extrinsic ageing. METHODS: Sun-exposed and non-exposed skin biopsies were collected from twenty-eight women devised in two groups (20-30 and ≥60 years old). The hydric organization and thermal transitions were determined by Differential Scanning Calorimetry (DSC)...
May 17, 2017: Skin Research and Technology
https://www.readbyqxmd.com/read/28513656/restoring-the-biophysical-properties-of-decellularized-patches-through-recellularization
#13
Gigi Chi Ting Au-Yeung, Udi Sarig, Hadar Sarig, Hanumakumar Bogireddi, Tomer Bronshtein, Limor Baruch, Avihai Spizzichino, Jacob Bortman, Boey Yin Chiang Freddy, Marcelle Machluf, Subbu S Venkatraman
Various extracellular matrix (ECM) scaffolds, isolated through decellularization, were suggested as ideal biomimetic materials for 'Functional tissue engineering' (FTE). The decellularization process comprises a compromise between damaging and preserving the ultrastructure and composition of ECM-previously shown to affect cell survival, proliferation, migration, organization, differentiation and maturation. Inversely, the effects of cells on the ECM constructs' biophysical properties, under physiological-like conditions, remain still largely unknown...
May 30, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28510051/proteins-in-binary-solvents
#14
REVIEW
Francesco Spinozzi, Paolo Mariani, Maria Grazia Ortore
Proteins in living organisms exist in complex aqueous solutions or embedded in membranes. In solution, proteins are surrounded by a tightly bound hydration layer, which is more ordered and less mobile than bulk water. As a consequence, water plays a major role in controlling protein structure stability, conformational flexibility, dynamics, and functionality, but it also appears that protein surface regulates the structuring of the surrounding water. The presence of cosolvents can modify the hydration layer characteristics and then the whole protein structural and dynamical properties...
June 2016: Biophysical Reviews
https://www.readbyqxmd.com/read/28504162/purification-of-glucomannan-from-salep-part-2-structural-characterization
#15
Abdullah Kurt, Talip Kahyaoglu
The aim of this study was to investigate the effects of different extraction temperatures (25, 55 and 85°C) on the structural characteristics of glucomannan (GM) purified from salep. All extraction conditions reduced protein content and molecular weight of salep. The effectiveness of removing the impurities and the highest purity of GM25 were observed with size exclusion profile. FTIR experiments demonstrated the presence of acetyl group, reduction of protein content and increment of polymer interaction of GM25...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28485052/evaluation-of-the-thermal-property-of-bovine-intramuscular-adipose-tissue-using-differential-scanning-calorimetry
#16
Noriyuki Kimura, Nana Nishimura, Nagako Iwama, Yoshito Aihara, Yasuki Ogawa, Yuji Miyaguchi
The thermal property of bovine intramuscular adipose tissue (IAT) was evaluated using differential scanning calorimetry (DSC) and compared with the melting point temperature (MP) of the fat extract of IAT, which was measured using the slip point method. The beef samples were classified according to the beef marbling score (BMS). Beef with a high BMS contained less protein than that with middle or low BMS. Beef with a high BMS contained significantly more fat than that with a low BMS (P < 0.05). The endothermic point temperature (EP) of IAT, measured by DSC, was significantly higher than the MP of IAT fat (P < 0...
May 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28474498/the-metabolic-potential-of-the-single-cell-genomes-obtained-from-the-challenger-deep-mariana-trench-within-the-candidate-superphylum-parcubacteria-od1
#17
Rosa León-Zayas, Logan Peoples, Jennifer F Biddle, Sheila Podell, Mark Novotny, James Cameron, Roger S Lasken, Douglas H Bartlett
Candidate phyla (CP) are broad phylogenetic clusters of organisms that lack cultured representatives. Included in this fraction is the candidate Parcubacteria superphylum. Specific characteristics that have been ascribed to the Parcubacteria include reduced genome size, limited metabolic potential, and exclusive reliance on fermentation for energy acquisition. The study of new environmental niches, such as the marine versus terrestrial subsurface, often expands the understanding of the genetic potential of taxonomic groups...
May 4, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28454364/personalized-therapy-tests-for-the-monitoring-of-chronic-lymphocytic-leukemia-development
#18
Małgorzata Rogalińska, Paweł Góralski, Jerzy Z Błoński, Paweł Robak, Jan Barciszewski, Aneta Koceva-Chyła, Henryk Piekarski, Tadeusz Robak, Zofia M Kilianska
There is individual variation in the course of disease development and response to therapy of patients with chronic lymphocytic leukemia (CLL). Novel treatment options for CLL include a new generation of purine analogs, antibodies and inhibitors of specific cell signaling pathways, which typically induce apoptosis or necrosis. A prospective analysis of patient blood samples revealed that a combination of four tests allowed the most appropriate and effective type of treatment to be selected prior to drug administration, and for the analysis of leukemic cell sensitivity to anticancer drug(s) during disease development...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28453980/effect-of-ultimate-ph-and-ageing-on-thermal-denaturation-of-bovine-muscle-proteins
#19
Juan D Rios-Mera, Jair Sebastião da Silva Pinto, Carmen J Contreras-Castillo
The thermal denaturation of proteins was evaluated in the natural state of bovine muscle using differential scanning calorimetry (DSC). The Longissimus lumborum muscle was selected according to ultimate pH (pHu) values classified into two groups: low pHu with values between 5.4 and 5.8, and intermediate pHu with values between 5.81 and 6.19. The muscles were cut and aged at 2°C up to 21d post mortem. The three maximum temperatures of denaturation (Tmax1, Tmax2, Tmax3) found in muscle were evaluated, showing higher thermal stability in the intermediate pHu group, which could be an indicator of protection of proteins against aggregation or enzymatic activity...
April 20, 2017: Meat Science
https://www.readbyqxmd.com/read/28450023/aggregation-of-gluten-proteins-in-model-dough-after-fibre-polysaccharide-addition
#20
Agnieszka Nawrocka, Monika Szymańska-Chargot, Antoni Miś, Agnieszka Z Wilczewska, Karolina H Markiewicz
FT-Raman spectroscopy, thermogravimetry and differential scanning calorimetry were used to study changes in structure of gluten proteins and their thermal properties influenced by four dietary fibre polysaccharides (microcrystalline cellulose, inulin, apple pectin and citrus pectin) during development of a model dough. The flour reconstituted from wheat starch and wheat gluten was mixed with the polysaccharides in five concentrations: 3%, 6%, 9%, 12% and 18%. The obtained results showed that all polysaccharides induced similar changes in secondary structure of gluten proteins concerning formation of aggregates (1604cm(-1)), H-bonded parallel- and antiparallel-β-sheets (1690cm(-1)) and H-bonded β-turns (1664cm(-1))...
September 15, 2017: Food Chemistry
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