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https://www.readbyqxmd.com/read/28516782/exploring-site-specific-n-glycosylation-of-hek293-and-plant-produced-human-iga-isotypes
#1
Kathrin Göritzer, Daniel Maresch, Friedrich Altmann, Christian Obinger, Richard Strasser
The full potential of recombinant Immunoglobulin A as therapeutic antibody is not fully explored, owing to the fact that structure-function relationships of these extensively glycosylated proteins are not well understood. Here, monomeric IgA1, IgA2m(1) and IgA2m(2) variants of the anti-HER2 antibody (IgG1) trastuzumab were expressed in glyco-engineered Nicotiana benthamiana plants and in human HEK293-6E cells. All three IgA isotypes were purified and subjected to biophysical and biochemical characterization...
May 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28516572/identification-of-ageing-biomarkers-in-human-dermis-biopsies-by-thermal-analysis-dsc-combined-with-fourier-transform-infrared-spectroscopy-ftir-atr
#2
R Tang, V Samouillan, J Dandurand, C Lacabanne, M-H Lacoste-Ferre, P Bogdanowicz, P Bianchi, A Villaret, F Nadal-Wollbold
BACKGROUND/PURPOSE: The purpose of this clinical study was to identify suitable biomarkers for a better understanding of the molecular and organizational changes in human dermis during intrinsic and extrinsic ageing. METHODS: Sun-exposed and non-exposed skin biopsies were collected from twenty-eight women devised in two groups (20-30 and ≥60 years old). The hydric organization and thermal transitions were determined by Differential Scanning Calorimetry (DSC)...
May 17, 2017: Skin Research and Technology
https://www.readbyqxmd.com/read/28513656/restoring-the-biophysical-properties-of-decellularized-patches-through-recellularization
#3
Gigi Chi Ting Au-Yeung, Udi Sarig, Hadar Sarig, Hanumakumar Bogireddi, Tomer Bronshtein, Limor Baruch, Avihai Spizzichino, Jacob Bortman, Boey Yin Chiang Freddy, Marcelle Machluf, Subbu S Venkatraman
Various extracellular matrix (ECM) scaffolds, isolated through decellularization, were suggested as ideal biomimetic materials for 'Functional tissue engineering' (FTE). The decellularization process comprises a compromise between damaging and preserving the ultrastructure and composition of ECM-previously shown to affect cell survival, proliferation, migration, organization, differentiation and maturation. Inversely, the effects of cells on the ECM constructs' biophysical properties, under physiological-like conditions, remain still largely unknown...
May 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28510051/proteins-in-binary-solvents
#4
REVIEW
Francesco Spinozzi, Paolo Mariani, Maria Grazia Ortore
Proteins in living organisms exist in complex aqueous solutions or embedded in membranes. In solution, proteins are surrounded by a tightly bound hydration layer, which is more ordered and less mobile than bulk water. As a consequence, water plays a major role in controlling protein structure stability, conformational flexibility, dynamics, and functionality, but it also appears that protein surface regulates the structuring of the surrounding water. The presence of cosolvents can modify the hydration layer characteristics and then the whole protein structural and dynamical properties...
June 2016: Biophysical Reviews
https://www.readbyqxmd.com/read/28504162/purification-of-glucomannan-from-salep-part-2-structural-characterization
#5
Abdullah Kurt, Talip Kahyaoglu
The aim of this study was to investigate the effects of different extraction temperatures (25, 55 and 85°C) on the structural characteristics of glucomannan (GM) purified from salep. All extraction conditions reduced protein content and molecular weight of salep. The effectiveness of removing the impurities and the highest purity of GM25 were observed with size exclusion profile. FTIR experiments demonstrated the presence of acetyl group, reduction of protein content and increment of polymer interaction of GM25...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28485052/evaluation-of-the-thermal-property-of-bovine-intramuscular-adipose-tissue-using-differential-scanning-calorimetry
#6
Noriyuki Kimura, Nana Nishimura, Nagako Iwama, Yoshito Aihara, Yasuki Ogawa, Yuji Miyaguchi
The thermal property of bovine intramuscular adipose tissue (IAT) was evaluated using differential scanning calorimetry (DSC) and compared with the melting point temperature (MP) of the fat extract of IAT, which was measured using the slip point method. The beef samples were classified according to the beef marbling score (BMS). Beef with a high BMS contained less protein than that with middle or low BMS. Beef with a high BMS contained significantly more fat than that with a low BMS (P < 0.05). The endothermic point temperature (EP) of IAT, measured by DSC, was significantly higher than the MP of IAT fat (P < 0...
May 8, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28474498/the-metabolic-potential-of-the-single-cell-genomes-obtained-from-the-challenger-deep-mariana-trench-within-the-candidate-superphylum-parcubacteria-od1
#7
Rosa León-Zayas, Logan Peoples, Jennifer F Biddle, Sheila Podell, Mark Novotny, James Cameron, Roger S Lasken, Douglas H Bartlett
Candidate phyla (CP) are broad phylogenetic clusters of organisms that lack cultured representatives. Included in this fraction is the candidate Parcubacteria superphylum. Specific characteristics that have been ascribed to the Parcubacteria include reduced genome size, limited metabolic potential, and exclusive reliance on fermentation for energy acquisition. The study of new environmental niches, such as the marine versus terrestrial subsurface, often expands the understanding of the genetic potential of taxonomic groups...
May 4, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28454364/personalized-therapy-tests-for-the-monitoring-of-chronic-lymphocytic-leukemia-development
#8
Małgorzata Rogalińska, Paweł Góralski, Jerzy Z Błoński, Paweł Robak, Jan Barciszewski, Aneta Koceva-Chyła, Henryk Piekarski, Tadeusz Robak, Zofia M Kilianska
There is individual variation in the course of disease development and response to therapy of patients with chronic lymphocytic leukemia (CLL). Novel treatment options for CLL include a new generation of purine analogs, antibodies and inhibitors of specific cell signaling pathways, which typically induce apoptosis or necrosis. A prospective analysis of patient blood samples revealed that a combination of four tests allowed the most appropriate and effective type of treatment to be selected prior to drug administration, and for the analysis of leukemic cell sensitivity to anticancer drug(s) during disease development...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28453980/effect-of-ultimate-ph-and-ageing-on-thermal-denaturation-of-bovine-muscle-proteins
#9
Juan D Rios-Mera, Jair Sebastião da Silva Pinto, Carmen J Contreras-Castillo
The thermal denaturation of proteins was evaluated in the natural state of bovine muscle using differential scanning calorimetry (DSC). The Longissimus lumborum muscle was selected according to ultimate pH (pHu) values classified into two groups: low pHu with values between 5.4 and 5.8, and intermediate pHu with values between 5.81 and 6.19. The muscles were cut and aged at 2°C up to 21d post mortem. The three maximum temperatures of denaturation (Tmax1, Tmax2, Tmax3) found in muscle were evaluated, showing higher thermal stability in the intermediate pHu group, which could be an indicator of protection of proteins against aggregation or enzymatic activity...
April 20, 2017: Meat Science
https://www.readbyqxmd.com/read/28450023/aggregation-of-gluten-proteins-in-model-dough-after-fibre-polysaccharide-addition
#10
Agnieszka Nawrocka, Monika Szymańska-Chargot, Antoni Miś, Agnieszka Z Wilczewska, Karolina H Markiewicz
FT-Raman spectroscopy, thermogravimetry and differential scanning calorimetry were used to study changes in structure of gluten proteins and their thermal properties influenced by four dietary fibre polysaccharides (microcrystalline cellulose, inulin, apple pectin and citrus pectin) during development of a model dough. The flour reconstituted from wheat starch and wheat gluten was mixed with the polysaccharides in five concentrations: 3%, 6%, 9%, 12% and 18%. The obtained results showed that all polysaccharides induced similar changes in secondary structure of gluten proteins concerning formation of aggregates (1604cm(-1)), H-bonded parallel- and antiparallel-β-sheets (1690cm(-1)) and H-bonded β-turns (1664cm(-1))...
September 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28429932/thermodynamics-of-the-thermal-denaturation-of-acid-molten-globule-state-of-cytochrome-c-indicate-a-reversible-high-temperature-oligomerization-process
#11
Shigeyoshi Nakamura, Tomonori Saotome, Akiko Nakazawa, Masao Fukuda, Yutaka Kuroda, Shun-Ichi Kidokoro
In this study, we performed differential scanning calorimetry (DSC) and pressure perturbation calorimetry (PPC) analysis of the thermal transition of cytochrome c from an acidic molten globule (MG) state with the protein concentrations of 0.5-18.2 mg/mL. DSC profiles were highly reversible and showed clear protein-concentration dependence, indicating that reversible oligomerization occurred accompanying the thermal transition from the MG state. The DSC and PPC data required at least a six-state model (MG1 ⇄ MG2 ⇄ D ⇄ (1)/2 I2 ⇄ (1)/3 I3 ⇄ (1)/4 I4) including three new oligomeric states: dimer (I2), trimer (I3), and tetramer (I4) in addition to the three monomeric states previously characterized...
May 9, 2017: Biochemistry
https://www.readbyqxmd.com/read/28411435/the-effect-of-surfactant-crystallization-on-partial-coalescence-in-o-w-emulsions
#12
Lucie Goibier, Sophie Lecomte, Fernando Leal-Calderon, Chrystel Faure
Partial coalescence is a ubiquitous instability in emulsions whose dispersed phase is partially crystallized. When emulsions are stabilized with proteins, interfacial stiffness and long-range repulsive surface forces hinder this type of instability. The addition of low molecular weight surfactants modifies the interfacial properties and surface forces, generally promoting partial coalescence. In the present work, various surfactants (Tween® 80, palmitic acid and monoglycerides) differing in their crystallization temperature were probed for their ability to induce partial coalescence in model O/W emulsions stabilized by sodium caseinate...
April 8, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28392389/fabrication-and-characterisation-of-soy-protein-isolate-grafted-dextran-biopolymer-a-novel-ingredient-in-spray-dried-soy-beverage-formulation
#13
Sareh Boostani, Mahmoud Aminlari, Marzieh Moosavi-Nasab, Mehrdad Niakosari, Gholamreza Mesbahi
Hybridization of macromolecules via the Maillard grafting reaction can punctuate specific forms of biopolymers, resulting in novel functionalities. The current study aimed at investigating and optimizing the conjugation of dextran-soy protein isolate (SPI), and to assess how Maillard modification can affect the properties of a spray-dried soy beverage. Analyses of SDS-PAGE, Glycation degree, SEM micrographs and FT-IR spectroscopy confirmed that the best condition for glycosylation operated on a pH value of 8...
April 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28391697/synthesis-and-thrombogenicity-evaluation-of-poly-3-methoxypropionic-acid-vinyl-ester-a-candidate-for-blood-compatible-polymers
#14
Kazuhiro Sato, Shingo Kobayashi, Asuka Sekishita, Miyuki Wakui, Masaru Tanaka
A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple two-step reaction, and then the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the nonthrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and enzyme-linked immunosorbent assay...
April 18, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28371560/differential-scanning-calorimetry-and-fluorimetry-measurements-of-monoclonal-antibodies-and-reference-proteins-effect-of-scanning-rate-and-dye-selection
#15
Brian E Lang, Kenneth D Cole
Differential scanning calorimetry (DSC) and differential scanning fluorimetry (DSF) were used to measure the transition temperatures of four proteins: RNase A, invertase, rituximab, and the NISTmAb (NIST Reference Material, RM 8671). The proteins were combined with several different fluorescent dyes for the DSF measurements. This study compares the results of DSC and DSF measurements of transition temperatures with different types of proteins, dye combinations, and thermal scan rates. As protein unfolding is often influenced by kinetic effects, we measured the transition temperatures of the proteins using DSC over a range of temperature scan rates and compared them to the data obtained from DSF over comparable temperature scan rates...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28328096/activation-of-nod-1-jnk-il-8-signal-axis-in-decidual-stromal-cells-facilitates-trophoblast-invasion
#16
Byung Jun Ryu, Jae Won Han, Ryang Hee Kim, Sohyun Yun, Tae Hyun Kim, Sung Eun Hur, Chul Jung Kim, Sung Ki Lee
Decidual stromal cells (DSCs) are known to regulate trophoblast invasion via unveiled mechanism yet. And nucleotide-binding oligomerization domain-containing protein 1 (NOD1) may influence on this DSC-trophoblast interaction. We investigated the mechanism underlying the DSC-mediated regulation of trophoblast invasion and the effect of NOD1 on their cross talk. Using human primary DSCs, BeWo cell invasion was measured. Cytokine secretion and MAP kinase signaling were examined in DSCs following treatment with NOD1 agonist, Tri-DAP...
March 22, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28315763/picw-orthologs-from-spruce-with-differential-freezing-tolerance-expressed-in-escherichia-coli
#17
Zhao Qihong, Liu Jie, Xu Xiao, Xu Qian, Gao Wei, Xu Jichen
Spruce can grow at an extra low temperature (LT), and is inferred with important antifreezing gene resources. The research here identified 4 different spruce varieties, named as PicW1, PicW2, PicM and PicK. Sequence alignment showed base-substitution and deficiency mutations among them with sequence identity between 97.61% and 99.25%. Each gene was transferred into E. coli, where protein was induced by IPTG (isopropyl-β-d-thiogalactoside). Strains cultured at -5°C showed the lethal dose 50% (LD-50) between 53h and 57h for the transgenic strains, but 35h for the control...
March 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28290299/in-vitro-digestibility-protein-composition-and-techno-functional-properties-of-saskatchewan-grown-yellow-field-peas-pisum-sativum-l-as-affected-by-processing
#18
Zhen Ma, Joyce I Boye, Xinzhong Hu
Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting. Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied. Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content...
February 2017: Food Research International
https://www.readbyqxmd.com/read/28287565/differential-scanning-calorimetry-a-method-for-assessing-the-thermal-stability-and-conformation-of-protein-antigen
#19
Ibrahim B Durowoju, Kamaljit S Bhandal, Jian Hu, Bruce Carpick, Marina Kirkitadze
Differential scanning calorimetry (DSC) is an analytical technique that measures the molar heat capacity of samples as a function of temperature. In the case of protein samples, DSC profiles provide information about thermal stability, and to some extent serves as a structural "fingerprint" that can be used to assess structural conformation. It is performed using a differential scanning calorimeter that measures the thermal transition temperature (melting temperature; Tm) and the energy required to disrupt the interactions stabilizing the tertiary structure (enthalpy; ∆H) of proteins...
March 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28287443/high-hydrostatic-pressure-hhp-induced-structural-modification-of-patatin-and-its-antioxidant-activities
#20
Rizwan Elahi, Tai-Hua Mu
Patatin represents a group of homologous primary storage proteins (with molecular weights ranging from 40 kDa to 45 kDa) found in Solanum tuberosum L. This group comprises 40% of the total soluble proteins in potato tubers. Here, patatin (40 kDa) was extracted from potato fruit juice using ammonium sulfate precipitation (ASP) and exposed to high hydrostatic pressure (HHP) treatment (250, 350, 450, and 550 MPa). We investigated the effect of HHP treatment on the structure, composition, heat profile, and antioxidant potential, observing prominent changes in HHP-induced patatin secondary structure as compared with native patatin (NP)...
March 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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