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https://www.readbyqxmd.com/read/28429932/thermodynamics-of-the-thermal-denaturation-of-acid-molten-globule-state-of-cytochrome-c-indicate-a-reversible-high-temperature-oligomerization-process
#1
Shigeyoshi Nakamura, Tomonori Saotome, Akiko Nakazawa, Masao Fukuda, Yutaka Kuroda, Shun-Ichi Kidokoro
In this study, we performed differential scanning calorimetry (DSC) and pressure perturbation calorimetry (PPC) analysis of the thermal transition of cytochrome c from an acidic molten globule (MG) state with the protein concentrations of 0.5-18.2 mg/mL. DSC profiles were highly reversible and showed clear protein-concentration dependence, indicating that reversible oligomerization occurred accompanying the thermal transition from the MG state. The DSC and PPC data required at least a six-state model including three new oligomeric states: dimer (I2), trimer (I3), and tetramer (I4) in addition to the three monomeric states previously characterized...
April 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28411435/the-effect-of-surfactant-crystallization-on-partial-coalescence-in-o-w-emulsions
#2
Lucie Goibier, Sophie Lecomte, Fernando Leal-Calderon, Chrystel Faure
Partial coalescence is a ubiquitous instability in emulsions whose dispersed phase is partially crystallized. When emulsions are stabilized with proteins, interfacial stiffness and long-range repulsive surface forces hinder this type of instability. The addition of low molecular weight surfactants modifies the interfacial properties and surface forces, generally promoting partial coalescence. In the present work, various surfactants (Tween® 80, palmitic acid and monoglycerides) differing in their crystallization temperature were probed for their ability to induce partial coalescence in model O/W emulsions stabilized by sodium caseinate...
April 8, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28392389/fabrication-and-characterisation-of-soy-protein-isolate-grafted-dextran-biopolymer-a-novel-ingredient-in-spray-dried-soy-beverage-formulation
#3
Sareh Boostani, Mahmoud Aminlari, Marzieh Moosavi-Nasab, Mehrdad Niakosari, Gholamreza Mesbahi
Hybridization of macromolecules via the Maillard grafting reaction can punctuate specific forms of biopolymers, resulting in novel functionalities. The current study aimed at investigating and optimizing the conjugation of dextran-soy protein isolate (SPI), and to assess how Maillard modification can affect the properties of a spray-dried soy beverage. Analyses of SDS-PAGE, Glycation degree, SEM micrographs and FT-IR spectroscopy confirmed that the best condition for glycosylation operated on a pH value of 8...
April 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28391697/synthesis-and-thrombogenicity-evaluation-of-poly-3-methoxypropionic-acid-vinyl-ester-a-candidate-for-blood-compatible-polymer
#4
Kazuhiro Sato, Shingo Kobayashi, Asuka Sekishita, Miyuki Wakui, Masaru Tanaka
A poly(vinyl acetate) derivative, poly(3-methoxypropionic acid vinyl ester) (PMePVE), was synthesized to develop a new candidate for blood compatible polymers. The monomer MePVE was synthesized by a simple 2-step reaction, and then, the MePVE was polymerized via free radical polymerization to obtain PMePVE. Human platelet adhesion tests were performed to evaluate the thrombogenicity, and the platelet adhesion was suppressed on the PMePVE-coated substrate. To determine the expression of the non-thrombogenicity of the PMePVE, the plasma protein adsorption and a conformationally altered state of fibrinogen were analyzed by a microBCA assay and ELISA...
April 10, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28371560/differential-scanning-calorimetry-and-fluorimetry-measurements-of-monoclonal-antibodies-and-reference-proteins-effect-of-scanning-rate-and-dye-selection
#5
Brian E Lang, Kenneth D Cole
Differential scanning calorimetry (DSC) and differential scanning fluorimetry (DSF) were used to measure the transition temperatures of four proteins: RNase A, invertase, rituximab, and the NISTmAb (NIST Reference Material, RM 8671). The proteins were combined with several different fluorescent dyes for the DSF measurements. This study compares the results of DSC and DSF measurements of transition temperatures with different types of proteins, dye combinations, and thermal scan rates. As protein unfolding is often influenced by kinetic effects, we measured the transition temperatures of the proteins using DSC over a range of temperature scan rates and compared them to the data obtained from DSF over comparable temperature scan rates...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28328096/activation-of-nod-1-jnk-il-8-signal-axis-in-decidual-stromal-cells-facilitates-trophoblast-invasion
#6
Byung Jun Ryu, Jae Won Han, Ryang Hee Kim, Sohyun Yun, Tae Hyun Kim, Sung Eun Hur, Chul Jung Kim, Sung Ki Lee
Decidual stromal cells (DSCs) are known to regulate trophoblast invasion via unveiled mechanism yet. And nucleotide-binding oligomerization domain-containing protein 1 (NOD1) may influence on this DSC-trophoblast interaction. We investigated the mechanism underlying the DSC-mediated regulation of trophoblast invasion and the effect of NOD1 on their cross talk. Using human primary DSCs, BeWo cell invasion was measured. Cytokine secretion and MAP kinase signaling were examined in DSCs following treatment with NOD1 agonist, Tri-DAP...
March 22, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28315763/picw-orthologs-from-spruce-with-differential-freezing-tolerance-expressed-in-escherichia-coli
#7
Zhao Qihong, Liu Jie, Xu Xiao, Xu Qian, Gao Wei, Xu Jichen
Spruce can grow at an extra low temperature (LT), and is inferred with important antifreezing gene resources. The research here identified 4 different spruce varieties, named as PicW1, PicW2, PicM and PicK. Sequence alignment showed base-substitution and deficiency mutations among them with sequence identity between 97.61% and 99.25%. Each gene was transferred into E. coli, where protein was induced by IPTG (isopropyl-β-d-thiogalactoside). Strains cultured at -5°C showed the lethal dose 50% (LD-50) between 53h and 57h for the transgenic strains, but 35h for the control...
March 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28290299/in-vitro-digestibility-protein-composition-and-techno-functional-properties-of-saskatchewan-grown-yellow-field-peas-pisum-sativum-l-as-affected-by-processing
#8
Zhen Ma, Joyce I Boye, Xinzhong Hu
Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting. Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied. Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content...
February 2017: Food Research International
https://www.readbyqxmd.com/read/28287565/differential-scanning-calorimetry-a-method-for-assessing-the-thermal-stability-and-conformation-of-protein-antigen
#9
Ibrahim B Durowoju, Kamaljit S Bhandal, Jian Hu, Bruce Carpick, Marina Kirkitadze
Differential scanning calorimetry (DSC) is an analytical technique that measures the molar heat capacity of samples as a function of temperature. In the case of protein samples, DSC profiles provide information about thermal stability, and to some extent serves as a structural "fingerprint" that can be used to assess structural conformation. It is performed using a differential scanning calorimeter that measures the thermal transition temperature (melting temperature; Tm) and the energy required to disrupt the interactions stabilizing the tertiary structure (enthalpy; ∆H) of proteins...
March 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28287443/high-hydrostatic-pressure-hhp-induced-structural-modification-of-patatin-and-its-antioxidant-activities
#10
Rizwan Elahi, Tai-Hua Mu
Patatin represents a group of homologous primary storage proteins (with molecular weights ranging from 40 kDa to 45 kDa) found in Solanum tuberosum L. This group comprises 40% of the total soluble proteins in potato tubers. Here, patatin (40 kDa) was extracted from potato fruit juice using ammonium sulfate precipitation (ASP) and exposed to high hydrostatic pressure (HHP) treatment (250, 350, 450, and 550 MPa). We investigated the effect of HHP treatment on the structure, composition, heat profile, and antioxidant potential, observing prominent changes in HHP-induced patatin secondary structure as compared with native patatin (NP)...
March 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28274869/effective-inhibition-of-protein-glycation-by-combinatorial-usage-of-limonene-and-aminoguanidine-through-differential-and-synergistic-mechanisms
#11
Madhav M Joglekar, Laxman N Bavkar, Srinivas Sistla, Akalpita U Arvindekar
Protein glycation is a major mechanism for establishing secondary complication in diabetes mellitus. Effective inhibition of this process can prevent progression of the disorder into secondary complications. Aminoguanidine (AMG) and limonene (LM) are known protein glycation inhibitors. The aim of the present study was to demonstrate their differential mechanisms of action and to study whether combinatorial therapy can act synergistically and lower dosage, and thereby lower toxicity in treatment of secondary complications in diabetes...
June 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28267324/emulsifying-properties-of-oxidatively-stressed-myofibrillar-protein-emulsion-gels-prepared-with-epigallocatechin-3-gallate-and-nacl
#12
Xianchao Feng, Lin Chen, Na Lei, Shuangxi Wang, Xinglian Xu, Guanghong Zhou, Zhixi Li
The dose-dependent effects of (-)-epigallocatechin-3-gallate (EGCG; 0, 100, or 1000 ppm) on the textural properties and stability of a myofibrillar protein (MP) emulsion gel were investigated. Addition of EGCG significantly inhibited formation of carbonyl but promoted the loss of both thiol and free amine groups. Addition of EGCG, particularly at 1000 ppm, initiated irreversible protein modifications, as evidenced by surface hydrophobicity changes, patterns in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and differential scanning calorimetry...
March 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28244103/purification-of-lactobacillus-acidophilus-surface-layer-protein-and-its-immunomodulatory-effects-on-raw264-7-cells
#13
Dandan Zhang, Mengting Wu, Yuxing Guo, Mingyue Xun, Wenwen Wang, Zhen Wu, Daodong Pan
BACKGROUND: Surface-layer proteins (SLP) have been found in the outermost layer of the cell wall in many types of lactobacillus are considered to be an important factor with respect to intestinal immunity. RESULTS: The present study compared the effects of SLP extracted by different concentrations of LiCl and carbamide, and subsequently identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, circular dichroism and differential scanning calorimetry...
February 28, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28212986/correlating-the-effects-of-antimicrobial-preservatives-on-conformational-stability-aggregation-propensity-and-backbone-flexibility-of-an-igg1-mab
#14
Jayant Arora, Sangeeta B Joshi, C Russell Middaugh, David D Weis, David B Volkin
Multidose formulations of biotherapeutics, which offer better dosage management and reduced production costs, require the addition of antimicrobial preservatives (APs). APs have been shown, however, to decrease protein stability in solution and cause protein aggregation. In this report, the effect of 4 APs, m-cresol, phenol, phenoxyethanol, and benzyl alcohol on conformational stability, aggregation propensity, and backbone flexibility of an IgG1 mAb, mAb-4, is investigated. Compared with no preservative control, each of the APs decreased the conformational stability of mAb-4 as measured by differential scanning calorimetry and extrinsic fluorescence spectroscopy...
February 14, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28201873/insights-into-the-formation-and-structures-of-starch-protein-lipid-complexes
#15
Shujun Wang, Mengge Zheng, Jinglin Yu, Shuo Wang, Les Copeland
The aim of the present study was to characterize the multiscale structures of ternary complexes of a model system of starch, fatty acid (FA), and β-lactoglobulin (βLG) prepared using a Rapid Visco Analyzer (RVA). The addition of βLG to starch-lauric acid or starch-oleic acid RVA pastes resulted in the increased intensity or occurrence of a new viscosity peak during cooling when the RVA protocol was repeated. The viscosity peak was attributed to the formation of starch-βLG-FA complexes. Differential scanning calorimetry (DSC) results showed clearly that the starch-βLG-FAs complex was formed as gelatinized starch was cooled in the presence of βLG and FAs...
February 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28199887/killing-colon-cancer-cells-through-pcd-pathways-by-a-novel-hyaluronic-acid-modified-shell-core-nanoparticle-loaded-with-rip3-in-combination-with-chloroquine
#16
Xueyan Hou, Chengli Yang, Lijing Zhang, Tingting Hu, Dan Sun, Hua Cao, Fan Yang, Gang Guo, Changyang Gong, Xiaoning Zhang, Aiping Tong, Rui Li, Yu Zheng
Due to extensive apoptosis defects and multidrug resistance, there is great interest regarding non-apoptotic programmed cell death (PCD) pathways, such as lysosomal-mediated programmed cell death (LM-PCD), necroptosis and autophagy. Because there is an intricate effector network among these PCD pathways, it is expected that they may act synergistically in cancer therapy. In this study, chloroquine (CQ) was found to significantly upregulate receptor-interacting protein kinase 3 (RIP3) expression, and RIP3 were involved in CQ-related autophagy...
April 2017: Biomaterials
https://www.readbyqxmd.com/read/28186169/functionalized-graphene-oxide-with-chitosan-for-protein-nanocarriers-to-protect-against-enzymatic-cleavage-and-retain-collagenase-activity
#17
Fatemeh Emadi, Abbas Amini, Ahmad Gholami, Younes Ghasemi
Proteins have short half-life because of enzymatic cleavage. Here, a new protein nanocarrier made of graphene oxide (GO) + Chitosan (CS) is proposed to successfully prevent proteolysis in protein and simultaneously retain its activity. Bovine serum albumin (BSA) and collagenase were loaded on GO and GO-CS to explore the stability and activity of proteins. SEM, AFM, TEM, DSC, UV-Vis, FT-IR, RBS, Raman, SDS-PAGE and zymography were utilized as characterization techniques. The protecting role of GO and GO-CS against enzymatic cleavage was probed by protease digestion analysis on BSA, where the protease solution was introduced to GO-BSA and GO-CS-BSA at 37 °C for 0...
February 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28163130/improved-thermal-stability-and-mechanical-properties-of-type-i-collagen-by-crosslinking-with-casein-keratin-and-soy-protein-isolate-using-transglutaminase
#18
Xiaomeng Wu, Yaowei Liu, Anjun Liu, Wenhang Wang
The inferior thermal- stability of collagen hinders its extensive application in food industry, including edible packaging. To improve the thermal- stability and mechanical properties of collagen, we attempted to crosslink collagen with some proteins possessing excellent thermal stability (i. e., casein, keratin and soy protein isolate (SPI)). Observed from the SDS- PAGE and particle size distribution, some complexes with higher molecule weight and relative bigger size particle occurred in the protein mixture, especially after TGase crosslinking...
February 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28071804/differential-scanning-calorimetry-analysis-of-the-effects-of-heat-and-pressure-on-protein-denaturation-in-soy-flour-mixed-with-various-types-of-plasticizers
#19
Meera Kweon, Louise Slade, Harry Levine
The effects of heat and pressure on protein denaturation in soy flour were explored by an experimental design that used pressure (atmospheric to 600 MPa), temperature (room to 90 °C), time (1 to 60 min), and type of aqueous plasticizer (NaCl, sucrose, betaine, and lactobionic acid (LBA)) as factors. When 50% (w/w) soy flour-water paste was high hydrostatic pressure (HHP)-treated for 20 min at 25 °C, the treatment at 200 MPa showed a small effect on denaturation of only the 7S soy globulin, but the treatment at 600 MPa showed a significant effect on denaturation of both the 7S and 11S soy globulins...
January 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28040569/thermal-and-optical-characterization-of-biologically-synthesized-zns-nanoparticles-synthesized-from-an-endophytic-fungus-aspergillus-flavus-a-colorimetric-probe-in-metal-detection
#20
Priyanka Uddandarao, Raj Mohan Balakrishnan
Nanostructured semiconductor materials are of great importance for several technological applications due to their optical and thermal properties. The design and fabrication of metal sulfide nanoparticles with tunable properties for advanced applications have drawn a great deal of attention in the field of nanotechnology. ZnS is a potential II-IV group material which is used in hetero-junction solar cells, light emitting diodes, optoelectronic devices, electro luminescent devices and photovoltaic cells. Due to their multiple applications, there is a need to elucidate their thermal and optical properties...
March 15, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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