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DSC protein

Bahman Nazari, Mohammad Amin Mohammadifar, Saeedeh Shojaee-Aliabadi, Ehsan Feizollahi, Leila Mirmoghtadaie
In this study, the effect of high power ultrasound (US) probe in varying intensities and times (18.4, 29.58, and 73.95 W/cm(2) for 5, 12.5 and 20 min respectively) on functional properties of millet protein concentrate (MPC) was investigated, and also the structural properties of best modified treatment were evaluated by FTIR, DSC, Zeta potential and SDS-PAGE techniques. The results showed the solubility in all US treated MPC was significantly (p < .05) higher than those of the native MPC. Foaming capacity of native MPC (271...
March 2018: Ultrasonics Sonochemistry
Punuri Jayasekhar Babu, Mukesh Doble, Ashok M Raichur
The synergistic wound healing and antibacterial activity of silver oxide nanoparticles embedded silk fibroin (Ag2O-SF) spuns is reported here. UV-Vis spectro photometric analysis of these spuns showed the surface plasmon resonance (SPR) confirming the formation of the silver oxide nanoparticles (Ag2O NPs) on the surface of the silk fibroin (SF). Scanning electron microscope (SEM) and Differential scanning calorimetry (DSC) also confirmed the presence of Ag2O NPs on surface of SF. X-ray diffraction (XRD) analysis revealed the crystalline nature of both SF and Ag2O-SF...
November 2, 2017: Journal of Colloid and Interface Science
Shingo Kobayashi, Miyuki Wakui, Yukihisa Iwata, Masaru Tanaka
A series of polyacrylates with different n-alkyl side chain lengths (one to six, and twelve carbons) and a ω-methoxy terminal group [poly(ω-methoxyalkyl acrylate): PMCxA] were prepared to study their nonthrombogenicity using human platelet adhesion, micro bicinchoninic acid (micro BCA) protein assay, and enzyme-linked immunosorbent assay (ELISA) tests. In all cases, human platelet adhesion was suppressed on the PMCxA coated substrates, and the number of human platelets adhered to the PMC3A [poly(3-methoxypropyl acrylate)] coated surface was comparable to that of commercialized nonthrombogenic coating agent poly(2-methoxyethyl acrylate) (PMEA, equal to PMC2A)...
November 13, 2017: Biomacromolecules
Sarah K Kendrick, Qi Zheng, Nichola C Garbett, Guy N Brock
BACKGROUND: DSC is used to determine thermally-induced conformational changes of biomolecules within a blood plasma sample. Recent research has indicated that DSC curves (or thermograms) may have different characteristics based on disease status and, thus, may be useful as a monitoring and diagnostic tool for some diseases. Since thermograms are curves measured over a range of temperature values, they are considered functional data. In this paper we apply functional data analysis techniques to analyze differential scanning calorimetry (DSC) data from individuals from the Lupus Family Registry and Repository (LFRR)...
2017: PloS One
Dongpu Li, Hongfei Yu, Qinxiong Lin, Yun Liu
A bioactive chemical constituent, doliroside A, from Chinese traditional herbal medicine Dolichos falcata Klein was isolated, purified and identified by 60% ethanol extraction, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy. Molecular interaction mechanism between doliroside and amyloid β42 protein was evaluated by thioflavin T fluorescence (ThT), circular dichroism (CD), atomic force microscope (AFM), and differential scanning calorimeter (DSC) from the aspects of kinetics, secondary structure, morphology, and thermodynamics, respectively...
2017: PloS One
Ellen Hackl, Joseph Darkwah, Geoff Smith, Irina Ermolina
L-arginine was introduced into protein-based freeze-dried formulations to study the ability of arginine to reduce/prevent from protein aggregation during manufacturing, storage and reconstitution of lyophilized protein-based pharmaceuticals. As L-arginine is known to be very hygroscopic, additional excipients which could provide a moisture buffering capacity need to be introduced into the formulation. In the first part of our study-excipient formulation development-the screening of a number of sugars/polyols has been done in order to select the best combination of excipients that, in a complex with L-arginine, can (i) produce freeze-dried cakes with elegant appearance, adequate mechanical properties and reconstitution times, and (ii) resist/minimise the moisture sorption...
October 18, 2017: AAPS PharmSciTech
P Bruździak, A Panuszko, E Kaczkowska, B Piotrowski, A Daghir, S Demkowicz, J Stangret
The enhancing effect on the water structure has been confirmed for most of the osmolytes exhibiting both stabilizing and destabilizing properties in regard to proteins. The presented work concerns osmolytes, which should be classified as "structure breaking" solutes: taurine and N,N,N-trimethyltaurine (TMT). Here, we combine FTIR spectroscopy, DSC calorimetry and DFT calculations to gain an insight into the interactions between osmolytes and two proteins: lysozyme and ubiquitin. Despite high structural similarity, both osmolytes exert different influence on protein stability: taurine is a stabilizer, TMT is a denaturant...
October 17, 2017: Amino Acids
Rezvan Shaddel, Javad Hesari, Sodeif Azadmard-Damirchi, Hamed Hamishehkar, Bahram Fathi-Achachlouei, Qingrong Huang
Protein-polysaccharide interactions offer opportunities for designing the new functional foods with applications in the food and pharmaceutical industries. In this work, we microencapsulated black raspberry water extracts by double emulsion technique prior to complex coacervation using gelatin and gum Arabic to diminish the instability of their anthocyanins (ANCs) as water soluble compounds, particularly under harsh processing and storage conditions. Subsequently, optical microscopy, moisture, hygroscopicity, solubility, particle size, loading capacity, zeta potential, color parameters (L*, a*, b*, C, H° and TCD), stability, Fourier transform infrared spectroscopy (FTIR), and thermal behavior were evaluated...
October 10, 2017: International Journal of Biological Macromolecules
Tsutomu Arakawa, Nasib Karl Maluf
Allantoin is widely used as a skin care agent and readily forms crystals, which were recently shown to bind endotoxins and high molecular weight aggregates in cell culture harvests. Here, we have investigated the effects of allantoin on thermal stability and aggregation of protein using ribonuclease, bovine serum albumin and lysozyme using temperature-regulated circular dichroism (CD) and differential scanning microcalorimetry (DSC). Ribonuclease showed no change in thermal stability and aggregation by the addition of allantoin...
October 7, 2017: International Journal of Biological Macromolecules
Ahmed A Abd El-Maksoud, Ismail H Abd El-Ghany, Hossam S El-Beltagi, Sampson Anankanbil, Chiranjib Banerjee, Steen V Petersen, Bianca Pérez, Zheng Guo
Multi-functional phenolic emulsifiers were prepared by covalently coupling β-Lactoglobulin (βLg) to caffeic acid (CA) using crosslinker chemistry at different pH conditions (pH 2.5, 6.0, and 8.5). The resulting bioconjugates were characterized by MALDI-TOF MS, differential scanning calorimetry (DSC), fluorescence-quenching, infrared and circular dichroism spectroscopies. Furthermore, the emulsifying and antioxidant properties of βLg-CA conjugates were evaluated and compared to native β-Lactoglobulin and the non-covalent β-lactoglobulin/caffeic complex (βLg/CA)...
February 15, 2018: Food Chemistry
Ali Asghar Rastegari, Behnaz Buzari, Abdol-Khalegh Bordbar
Pepsin is generally used in the preparation of F(ab)2 fragments from antibodies. The antibodies that are one of the largest and fastest growing categories of bio- pharmaceutical candidates. Differential scanning calorimetric is principally suitable method to follow the energetics of a multi-domain, fragment to perform a more exhaustive description of the thermodynamics in an associating system. The thermodynamical models of analysis include the construction of a simultaneous fitting of a theoretical expression...
March 2017: Biochemistry and Biophysics Reports
Jayalakshmi Krishnamoorthi, Pasiyappazham Ramasamy, Vairamani Shanmugam, Annaian Shanmugam
Type I collagen from outer skin of Sepia pharaonis was extracted and partially characterized. Yield of Acid Soluble Collagen (ASC) and Pepsin Soluble Collagen (PSC) were calculated as 1.66% and 3.93% and the total protein content of ASC and PSC were found as 18.4% and 48.6%. FT-IR spectrum of ASC and PSC recorded 12 and 14 peaks, respectively. (1)H NMR spectrum of ASC showed singlets at 1.23 ppm, 3.1 ppm, 3.55 ppm and 3.7 ppm and PSC at 1.23 ppm and 2.08 ppm. The molecular weight for ASC was calculated as 102 kDa and for PSC as 110, 108 and 102 kDa through SDS-PAGE...
July 2017: Biochemistry and Biophysics Reports
Hossein Rajabinejad, Alessia Patrucco, Rosalinda Caringella, Alessio Montarsolo, Marina Zoccola, Pier Davide Pozzo
The interest towards microcapsules based on non-toxic, biodegradable and biocompatible polymers, such as proteins, is increasing considerably. In this work, microcapsules were prepared using water soluble keratin, known as keratoses, with the aim of encapsulating hydrophilic molecules. Keratoses were obtained via oxidizing extraction of pristine wool, previously degreased by Soxhlet. In order to better understand the shell part of microcapsules, pristine wool and obtained keratoses were investigated by FT-IR, gel-electrophoresis and HPLC...
January 2018: Ultrasonics Sonochemistry
David Santiago-Ramos, Juan de Dios Figueroa-Cárdenas, José Juan Véles-Medina, Ricardo Salazar
The aim of this work was to study the effect of three nixtamalization processes using wood ashes, Ca(OH)2, and CaCl2 on the physicochemical properties of beans. Raw beans had C-type starch, 10.10% resistant starch (RS), and two DSC endotherms: 1) starch gelatinization, and 2) melting of amylose-lipid complexes plus protein denaturation. Nixtamalization increased the Ca and Fe content, decreased the RS content to 4.19-4.43%, and produced starch retrogradation. DSC and FT-IR analysis of the flours evidenced a "stabilizing" effect of cooking with NaCl and CaCl2 on bean proteins...
February 1, 2018: Food Chemistry
Ruoxin Ruan, Kuang-Ren Chung, Hongye Li
Sterol regulatory element binding proteins (SREBPs) are required for sterol homeostasis in eukaryotes. Activation of SREBPs is regulated by the Dsc E3 ligase complex in Schizosaccharomyces pombe and Aspergillus spp. Previous studies indicated that an SREBP-coding gene PdsreA is required for fungicide resistance and ergosterol biosynthesis in the citrus postharvest pathogen Penicillium digitatum. In this study, five genes, designated PddscA, PddscB, PddscC, PddscD, and PddscE encoding the Dsc E3 ligase complex were characterized to be required for fungicide resistance, ergosterol biosynthesis and CoCl2 tolerance in P...
December 2017: Microbiological Research
Ahmed S Zidan, Sabir M Aqueel, Alaadin Alayoubi, Adil Mohammad, Jinhui Zhang, Ziyaur Rahman, Patrick Faustino, Richard T Lostritto, Muhammad Ashraf
Leachables derived from multi-component drug-device syringe systems can result in changes to the quality of drug products. Diphenylguanidine (DPG), a leachable released from styrene butadiene rubber syringe plungers, interacts with Oxytocin to form protein-adducts. This study investigated the mechanism and kinetics of this interaction in both solid and solution states through in-vitro tests and spectroscopic methods For solid state interaction, the protein-adducts with DPG were characterized using SEM, XRD, DSC, FTIR, (13)C ss NMR, and dissolution analysis...
September 19, 2017: International Journal of Pharmaceutics
Yunlong Shen, Yuxi Wang, Xiaohua Jiang, Liang Lu, Chengdi Wang, Wenxin Luo, Yongxia Zhang, Pei Li, Zhengwei Du, Tengfei Dai, Congcong Wu, Aiping Fang, Yuqin Yao, Qian Peng, Jinliang Yang
Human epididymis protein-4 (HE4) may serve as a putative biomarker for the early diagnosis, therapy and especially prognosis of ovarian, lung and breast cancer. Detection and targeting of HE4 using the anti-HE4 antibody could be one of the effective strategies for the cancer diagnosis and treatment in clinical practice. In this study, a high-efficiency expression system was established to purify recombinant HE4. We obtained high purity HE4 in 400 mg quantity from 1 L culture supernatant of HEK293F cells. CCK-8 and cell cycle assays indicated that the purified recombinant HE4 protein could promote SKOV3 cell cycle and proliferation at the concentration of 0...
January 2018: Protein Expression and Purification
Giovanna Di Nardo, Giuseppe Cimicata, Roberta Baravalle, Valentina Dell'Angelo, Alberto Ciaramella, Gianluca Catucci, Piero Ugliengo, Gianfranco Gilardi
Aromatase catalyzes the biosynthesis of estrogens from androgens. Owing to the physiological importance of this conversion of lipophilic substrates, the interaction with the lipid bilayer for this cytochrome P450 is crucial for its dynamics that must allow an easy access to substrates and inhibitors. Here, the aromatase-anastrozole interaction is studied by combining computational methods to identify possible access/egress routes with the protein inserted in the membrane and experimental tools aimed at the investigation of the effect of the inhibitor on the protein conformation...
September 19, 2017: Biotechnology and Applied Biochemistry
Liya Niu, Leiyan Wu, Jianhui Xiao
The retrogradation of gelatinized rice starch (GRS) during the shelf life of a product is the biggest barrier related to starch-containing foods. The objective of this study was to produce rice bran protein hydrolysate (RBPH) using proteolytic enzymes (alcalase, flavourzyme, protamex, neutrase, bromelain, papain and trypsin) to suppress the retrogradation of GRS and understand the physical phenomena underlying the reduced retrogradation of GRS by RBPH during short- and long-term storage. Mixtures of GRS incorporated with Protamex-hydrolyzed rice bran protein at 1h (PRBPH-1) at a degree of hydrolysis of 15...
November 1, 2017: Carbohydrate Polymers
Umar Bacha, Muhammad Nasir, Sanaullah Iqbal, Aftab Ahmad Anjum
β-Glucan is a dietary fibre, found in many natural sources, and controls chronic metabolic diseases effectively. However, β-glucan from the yeast has rarely been investigated. Objectively, conditions were optimized to isolate β-glucan from the yeast (max. 66% yield); those optimized conditions included 1.0 M NaOH, pH 7.0, and 90°C. The purity and identity of the isolated β-glucan were characterized through FT-IR, SEM, DSC, and physicofunctional properties. The obtained results from DSC revealed highly stable β-glucan (m...
2017: BioMed Research International
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