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DSC protein

Xiaomeng Sun, Cuina Wang, Hao Wang, Mingruo Guo
Powdered infant formula is usually manufactured by ingredients mixing, homogenization, pasteurization, evaporation and spray drying. Effects of unit operations on the microstructure, thermal properties and other characteristics of preterm infant formula, fat (F), serum (S), and pellet (P) fractions on centrifugation were investigated using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Differential Scanning Calorimetry (DSC), and Fourier Transform Infrared (FTIR) spectroscopy. After homogenization, particles which may be casein and denatured whey proteins were observed on the surface of F fraction in microstructure images...
May 10, 2018: Journal of Food Science
Maryam Amini Pouya, Behnaz Daneshmand, Shabnam Aghababaie, Homa Faghihi, Alireza Vatanara
We aimed to prepare spray-freeze-dried powder of IgG considering physicochemical stability and aerodynamic aspects. Spray-freeze drying (SFD) exposes proteins to various stresses which should be compensated by suitable stabilizers. The competence of cyclodextrins (CDs), namely beta-cyclodextrin (βCD) and hydroxypropyl βCD (HPβCD), at very low concentrations, was investigated in the presence of separate mannitol- and trehalose-based formulations. Spray-freeze-dried preparations were quantified in terms of monomer recovery and conformation by size exclusion chromatography (SEC-HPLC) and Fourier transform infrared (FTIR) spectroscopy, respectively...
May 8, 2018: AAPS PharmSciTech
M Kristiawan, V Micard, P Maladira, C Alchamieh, J-E Maigret, A-L Réguerre, M A Emin, G Della Valle
Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg). Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC). Foam density dropped from 820 to 85 kg/m3 with increase in SME and T (R2  ≥ 0...
June 2018: Food Research International
Salman Shahid, Ashish Kabra, Surbhi Mundra, Ravi Kant Pal, Sarita Tripathi, Anupam Jain, Ashish Arora
BACKGROUND: Bacterial peptidyl-tRNA hydrolase (Pth) is an essential enzyme that alleviates tRNA starvation by recycling prematurely dissociated peptidyl-tRNAs. The specificity of Pth for N-blocked-aminoacyl-tRNA has been proposed to be contingent upon conserved residue N14 forming a hydrogen bond with the carbonyl of the first peptide bond in the substrate. M71 is involved in forming a conserved hydrogen bond with N14. Other interactions facilitating this recognition are not known. METHODS: The structure, dynamics, and stability of the M71A mutant of Pth from Vibrio cholerae (VcPth) were characterized by X-ray crystallography, NMR spectroscopy, MD simulations and DSC...
May 4, 2018: Biochimica et Biophysica Acta
Sara Vieira, Albina R Franco, Emanuel M Fernandes, Sara Amorim, Helena Ferreira, Ricardo A Pires, Rui L Reis, Albino Martins, Nuno M Neves
Fish sarcoplasmic proteins (FSP) constitute around 25-30% of the total fish muscle protein. As the FSP are water soluble, FSP were isolated from fresh cod (Gadus morhua) by centrifugation. By SDS-PAGE, it was possible to determine the composition of FSP extracts (FSP-E). The FSP-E undergo denaturation at 44.12 ± 2.34° C, as characterized by differential scanning calorimetry thermograms (DSC). The secondary structure of FSP-E is mainly composed by α-helix structure, as determined by circular dichroism...
April 5, 2018: Colloids and Surfaces. B, Biointerfaces
Medhat Wahba Shafaa, Amany Hasan Elshazly, Amira Zaki Dakrory, Maha Reda Elsyed
Purpose: To stress the influence of Coenzyme Q10 (CoQ10) on the structural properties of liposomes as model membranes and to investigate the possible role of CoQ10 or CoQ10 doped in liposomes when topically instilled as eye drops, in preventing cataract. Methods: The molecular interaction between liposomes and Coenzyme Q10 was examined using differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR). Rat pups were randomly divided into six groups comprising 15 pups. Group (1), control group...
March 2018: Advanced Pharmaceutical Bulletin
Lucia Ricci, Eleonora Umiltà, Maria Cristina Righetti, Tiziana Messina, Chiara Zurlini, Angela Montanari, Simona Bronco, Monica Bertoldo
BACKGROUND: Peas, lentils, faba beans, chickpeas and beans proteins are potentially renewable raw materials for bioplastic production that can be obtained from agriculture waste. Plastics are usually processed under heating and thus the thermal stability is a mandatory requirement for the application. In this study the thermal behaviour of several legume proteins isolates at different purity degree were investigated. RESULTS: Proteins extracted from legumes exhibited thermal stability maxima for chickpeas, minimum for beans proteins and decreased with the decrease of the protein purity in the 88 % - 30 % range...
April 16, 2018: Journal of the Science of Food and Agriculture
Aditya V Gandhi, Keith J Arlotta, Hsiao-Nung Chen, Shawn C Owen, John F Carpenter
The commercially available antibody-drug conjugate (ADC) product, Kadcyla® is synthesized using a two-step reaction, wherein the linker is conjugated to native lysines on the monoclonal antibody (mAb) in step 1, followed by drug conjugation to the linker-modified antibody in step 2. In our study, we synthesized a lysine conjugated ADC (Syn-ADC) on the same trastuzumab scaffold as Kadcyla® using a one-step reaction. Mass spectrometry of both products revealed a sub-population of Kadcyla® containing free linkers conjugated to the mAb, but not conjugated to the drug, which were absent in the one-step reaction ADC product...
April 4, 2018: Journal of Pharmaceutical Sciences
Antonio José Ortiz-Ruiz, Juan de Dios Teruel-Fernández, Luis Alberto Alcolea-Rubio, Ana Hernández-Fernández, Yolanda Martínez-Beneyto, Francesc Gispert-Guirado
BACKGROUND: the aim was to study differences between crystalline nanostructures from the enamel and dentin of human, bovine, porcine, and ovine species. METHODS: dentine and enamel fragments extracted from sound human, bovine, porcine and ovine incisors and molars were mechanically ground up to a final particle size of < 100μm. Samples were analyzed using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC)...
March 28, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
Aman Kashyap, Rupinder Kaur, Ashish Baldi, Upendra Kumar Jain, Ramesh Chandra, Jitender Madan
Chloroquine diphosphate (CHQ) is primarily used for the treatment of Plasmodium falciparum malaria at the dose of 500 mg orally or 10 mg/kg parenterally. However, point mutations in Plasmodiumfalciparum chloroquine resistance transporter (PfCRT) protein and Plasmodium falciparum multidrug resistance protein 1 (Pfmdr1) localized in digestive vacuole membrane, are responsible for CHQ resistance. Therefore, in present investigation, dextran nanoparticles bearing chloroquine diphosphate (CHQ-DEX-NPs) were formulated by solvent diffusion method of size below 70 nm with zeta-potential of -20...
March 20, 2018: International Journal of Biological Macromolecules
K Jena, J P Pandey, Ruchi Kumari, A K Sinha, V P Gupta, G P Singh
The present study, investigates the properties of sericin extracted from tasar silk fiber waste (TSFW). Separation of residual sericin from TSFW was confirmed by scanning electron microscope (SEM). The molecular weight distribution of sericin was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results suggested that TSFW sericin represented a family of proteins with wide-ranging molecular weight distribution (11-245 kDa). Structural determination by FTIR revealed the presence of both α-helical and β-sheet structures...
March 14, 2018: International Journal of Biological Macromolecules
Jonas V Schaefer, Erik Sedlák, Florian Kast, Michal Nemergut, Andreas Plückthun
Biophysical properties of antibody-based biopharmaceuticals are a critical part of their release criteria. In this context, finding the appropriate formulation is equally important as optimizing their intrinsic biophysical properties through protein engineering, and both are mutually dependent. Most previous studies have empirically tested the impact of additives on measures of colloidal stability, while mechanistic aspects have usually been limited to only the thermodynamic stability of the protein. Here we emphasize the kinetic impact of additives on the irreversible denaturation steps of immunoglobulins G (IgG) and their antigen-binding fragments (Fabs), as these are the key committed steps preceding aggregation, and thus especially informative in elucidating the molecular parameters of activity loss...
March 14, 2018: MAbs
Krešimir Mastanjević, Dragan Kovačević
BACKGROUND: Possible new additives for surimi-like products made from chicken meat, which could improve its functional properties during frozen storage, are the subject of much research. The use of dietary fibre in surimi-like products made from chicken meat has not been extensively studied. The objective of this study was to determine the effectiveness of barley bran flour in stabilizing chicken myofibrillar proteins during frozen storage and maintaining its functionality. METHODS: Surimi-like material (chicken myofibrillar protein concentrate &ndash; CMPC) from mechanically deboned chicken meat was mixed with barley bran flour (0&ndash;6%) and stored in a freezer for 30, 60, 90 and 180 days...
January 2018: Acta Scientiarum Polonorum. Technologia Alimentaria
Bruna Carbas, Shivani Pathania, Ana Castanho, Diana Lourenço, Isabel Mota Veiga, Maria Carlota Vaz Patto, Carla Brites
The present study was aimed at studying the physico-chemical and functional properties of 31 Portuguese common bean varieties. In addition, the whole bean flours (WBF) and starch isolates (SI) of three representative bean varieties and their rice: bean blends (70:30; 50:50) were assessed for amylose content, thermal and pasting properties in view of supplementation in rice based processed foods. Bean varieties showed significant differences in protein content (20.78-27.10%), fat content (1.16-2.18%), hydration capacity (95...
March 2018: Journal of Food Science and Technology
María E Latorre, Diego E Velázquez, Peter P Purslow
The thermal denaturation of collagen the perimysium of intramuscular connective tissue isolated from bovine Semitendinosus (ST) and Perctoralis profundus (PP) muscles was investigated using a range of heating rates in differential scanning calorimetry (DSC) and analyzed by application of the Kissinger-Akahira-Sunose (KAS) and Lumry-Eyring models. Thermograms showed a broadening of endotherms and a shift towards higher temperatures as the thermal scanning rate increased. These features are consistent with the two-step process of a reversible transition between native and unfolded collagen molecules followed by an irreversible transition between unfolded and denatured states...
July 1, 2018: International Journal of Biological Macromolecules
James R Banigan, Maureen Leninger, Ampon Sae Her, Nathaniel J Traaseth
It is known that the lipid composition within a cellular membrane can influence membrane protein structure and function. In this Article, we investigated how structural changes to a membrane protein upon substrate binding can impact the lipid bilayer. To carry out this study, we reconstituted the secondary active drug transporter EmrE into a variety of phospholipid bilayers varying in headgroup and chain length and carried out differential scanning calorimetry (DSC) and solid-state NMR experiments. The DSC results revealed a difference in cooperativity of the lipid phase transition for drug-free EmrE protonated at glutamic acid 14 (i...
March 1, 2018: Journal of Physical Chemistry. B
Jacqueline Horn, Wolfgang Friess
The collapse temperature (Tc) and the glass transition temperature of freeze-concentrated solutions (Tg') as well as the crystallization behavior of excipients are important physicochemical characteristics which guide the cycle development in freeze-drying. The most frequently used methods to determine these values are differential scanning calorimetry (DSC) and freeze-drying microscopy (FDM). The objective of this study was to evaluate the optical fiber system (OFS) unit as alternative tool for the analysis of Tc, Tg' and crystallization events...
2018: Frontiers in Chemistry
Mengge Zheng, Chen Chao, Jinglin Yu, Les Copeland, Shuo Wang, Shujun Wang
The effects of chain length and degree of unsaturation of fatty acids (FAs) on structure and in vitro digestibility of starch-protein-FA complexes were investigated in model systems. Studies with the rapid visco analyzer (RVA) showed that the formation of ternary complex resulted in higher viscosities than those of binary complex during the cooling and holding stages. The results of differential scanning calorimetry (DSC), Raman, and X-ray diffraction (XRD) showed that the structural differences for ternary complexes were much less than those for binary complexes...
February 28, 2018: Journal of Agricultural and Food Chemistry
Xinxin Wang, Mingyuan Du, Guobin Mao, Jiao Zheng, Jinyang Chen, Xinghu Ji, Zhike He
A new kind of polymeric material (PNIPAAm-co-SA) was prepared by conjugating a thermosensitive polymer, Poly (N-isopropylacrylamide) (PNIPAAm) with streptavidin (SA). This smart prepared composite displayed a controllable conformation change between an expanded and a collapsed form, below or above its lower critical solution temperature (LCST). Differential scanning calorimetry (DSC) analysis demonstrated that the PNIPAAm-co-SA bioconjugate showed the same LCST as the original synthetic polymer, PNIPAAm, which was also 32°C...
May 1, 2018: Talanta
Bibekananda Kar, Preeti Verma, Riaan den Haan, Ashwani Kumar Sharma
In this study, the effect of N-glycosylation on the conformational and functional stability of Putranjiva roxburghii family 1 β-glucosidase (PRGH1) enzyme was investigated. The deglycosylation of PRGH1 was carried out by using PNGase F enzyme and confirmed by SDS-PAGE and carbohydrate estimation. Comparative analysis with respect to enzyme activity, stability and aggregation behaviour was carried out for the glycosylated and deglycosylated PRGH1. The deglycosylation of PRGH1 affected enzyme activity to a certain extent only where Km was not affected but a slight reduction in Vmax for various substrates was observed...
June 2018: International Journal of Biological Macromolecules
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