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DSC protein

Meera Kweon, Louise Slade, Harry Levine
The effects of heat and pressure on protein denaturation in soy flour were explored by an experimental design that used pressure (atmospheric to 600 MPa), temperature (room to 90 °C), time (1 to 60 min), and type of aqueous plasticizer (NaCl, sucrose, betaine, and lactobionic acid (LBA)) as factors. When 50% (w/w) soy flour-water paste was high hydrostatic pressure (HHP)-treated for 20 min at 25 °C, the treatment at 200 MPa showed a small effect on denaturation of only the 7S soy globulin, but the treatment at 600 MPa showed a significant effect on denaturation of both the 7S and 11S soy globulins...
January 10, 2017: Journal of Food Science
Priyanka Uddandarao, Raj Mohan Balakrishnan
Nanostructured semiconductor materials are of great importance for several technological applications due to their optical and thermal properties. The design and fabrication of metal sulfide nanoparticles with tunable properties for advanced applications have drawn a great deal of attention in the field of nanotechnology. ZnS is a potential II-IV group material which is used in hetero-junction solar cells, light emitting diodes, optoelectronic devices, electro luminescent devices and photovoltaic cells. Due to their multiple applications, there is a need to elucidate their thermal and optical properties...
December 18, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Carlos A Núñez-Álvarez, Diego F Hernández-Ramírez, Araceli Martinez-Castillo, Virginia Pascual Ramos, Javier Cabiedes, Alicia Ortega, Antonio R Cabral
: Homozygote genotype V(247) of the β2-glycoprotein-I (β2GP-I) gene has been associated with anti-β2GP-I and thrombosis in patients with primary anti-phospholipid syndrome APS (PAPS). However, the cellular immune response to β2GP-I(247) has been little studied. OBJECTIVE: To evaluate the immune cellular proliferation in response to native and non-native β2GP-I(247) valine/leucine phenotype from Mexican patients with PAPS. METHODS: We studied 10 patients with PAPS and 10 healthy control subjects (HC)...
December 27, 2016: Human Immunology
Aruna Rani, Rwivoo Baruah, Arun Goyal
Chicken keel bone cartilage was explored for cheaper and sustainable source for isolation of chondroitin sulphate (CS) for its future use in tissue engineering and pharmaceutical industry. HPSEC analysis displayed two peaks of 100kDa for CS-keel polysaccharide and 1kDa for protein. DLS analysis of CS-keel displayed polydispersity. CS-keel yield was 15% and 53±5% uronic acid content. The quantified percentages of UA-GalNAc4S and UA-GalNAc6S disaccharide in CS-keel were 58% and 42%, respectively. FT-IR identified CS-keel to be chondroitin 4-sulphate...
March 1, 2017: Carbohydrate Polymers
Jae Geun Song, Sang Hoon Lee, Hyo-Kyung Han
This study aimed to evaluate aminoclay (3-aminopropyl-functionalized magnesium phyllosilicate) as an effective protectant for the stabilization of protein formulation in freeze-drying. Bovine serum albumin (BSA), as a model protein, was freeze-dried with aminoclay at various concentrations, and the effects of aminoclay on the structural stability of proteins were compared with those of the conventional stabilizers. The structural characteristics of the protein were determined by size exclusion chromatography (SEC), circular dichroism (CD), and Fourier transform infrared (FTIR) spectroscopy...
2016: International Journal of Nanomedicine
Valeria Guzmán-Luna, Andrea G Quezada, A Jessica Díaz-Salazar, Nallely Cabrera, Ruy Pérez-Montfort, Miguel Costas
The effect of specific residues on the kinetic stability of two closely related triosephosphate isomerases (from Trypanosoma cruzi, TcTIM and Trypanosoma brucei, TbTIM) have been studied. Based on a comparison of their β-turn occurrence, we engineered two chimerical enzymes where their super secondary β-loop-α motifs 2 ((βα)2 ) were swapped. Differential scanning calorimetry (DSC) experiments showed that the (βα)2 motif of TcTIM inserted into TbTIM (2Tc) increases the kinetic stability. On the other hand, the presence of the (βα)2 motif of TbTIM inserted into TcTIM (2Tb) gave a chimerical protein difficult to purify in soluble form and with a significantly reduced kinetic stability...
December 21, 2016: Proteins
Charlene Priscila Kiilll, Hernane da Silva Barud, Sílvia Helena Santagneli, Sidney José Lima Ribeiro, Amélia M Silva, Agnieszka Tercjak, Junkal Gutierrez, Andressa Maria Pironi, Maria Palmira Daflon Gremião
Chitosan nanoparticles have been extensively studied for both drug and protein/peptide delivery. The aim of this study was to develop an optimized chitosan nanoparticle, by ionotropic gelation method, using 3(2) full factorial design with a novel polyanion, sodium polyphosphate, well known under the trade name Graham salt. The effects of these parameters on the particle size, zeta potential, and morphology and association efficiency were investigated. The optimized nanoparticles showed an estimated size of 166...
February 10, 2017: Carbohydrate Polymers
Elahe Mobarak Qamsari, Rouha Kasra Kermanshahi, Mohammad Erfan, Parinaz Ghadam, Soroush Sardari, Neda Eslami
In this work, some important characteristics of surface layer (S-layer) proteins extracted from two new and native Lactobacillus strains, L.brevis KM3 and L.brevis KM7, were investigated. The presence of S-layer on the external surface of L.brevis KM3 was displayed by thin sectioning and negative staining. SDS-PAGE analysis were shown same dominant protein bands approximately around 48kDa for both S-layer proteins. Moreover, the S-layer reappeared when LiCl treated cells were allowed to grow again. Protein secondary structure and thermal behavior were evaluated by using circular dichroism (CD) and differential scanning calorimetry (DSC), respectively...
February 2017: International Journal of Biological Macromolecules
Samet Bayraktar, Pascal Jungbluth, René Deenen, Jan Grassmann, Johannes Schneppendahl, Daphne Eschbach, Armin Scholz, Joachim Windolf, Christoph V Suschek, Vera Grotheer
Mesenchymal stromal cells (MSC) play a pivotal role in modern therapeutic approaches in bone healing disorders. Although bone marrow-derived MSC are most frequently used, the knowledge that many other adult tissues represent promising sources for potent MSC has gained acceptance. In the present study, the osteogenic differentiation potential of porcine skin fibroblasts (FB) as well as bone marrow- (BMSC), adipose tissue- (ASC), and dental pulp-derived stromal cells (DSC) were evaluated. However, additional application of BMP-2 significantly elevated the delayed osteogenic differentiation capacity of ASC and FB cultures...
December 14, 2016: Journal of Tissue Engineering and Regenerative Medicine
Simon F Eskildsen, Louise Gyldensted, Kartheeban Nagenthiraja, Rune B Nielsen, Mikkel Bo Hansen, Rikke B Dalby, Jesper Frandsen, Anders Rodell, Carsten Gyldensted, Sune N Jespersen, Torben E Lund, Kim Mouridsen, Hans Brændgaard, Leif Østergaard
Alzheimer's disease (AD) is characterized by the accumulation of hyperphosphorylated tau and neurotoxic Aβ in the brain parenchyma. Hypoxia caused by microvascular changes and disturbed capillary flows could stimulate this build-up of AD-specific proteins in the brain. In this study, we compared cerebral microcirculation in a cohort of AD and mild cognitive impairment (MCI) patients with that of age-matched controls, all without a history of diabetes or of hypertension for more than 2 years, using dynamic susceptibility contrast magnetic resonance imaging (DSC-MRI)...
February 2017: Neurobiology of Aging
Xuejiao Wang, Niamat Ullah, Xuchun Sun, Yan Guo, Lin Chen, Zhixi Li, Xianchao Feng
Biocomposite films were manufactured by combining protein extracted from buckwheat distiller's dried grains with bacterial cellulose (BC). The film microstructures showed that BC is compatible with protein matrix and endows the film with high rigidity. Differential scanning calorimetry (DSC) showed that BC can promote thermal stability of the composite films. BC promoted the transition from a Newtonian to a non-Newtonian fluid and the shear thinning behavior of protein-BC solution. Fourier Transform Infrared (FTIR) spectroscopy showed the main functional groups' absorption peaks shifted to lower wavenumbers...
November 28, 2016: International Journal of Biological Macromolecules
Sulabh Patel, Jan Olaf Stracke, Ulrike Altenburger, Hanns-Christian Mahler, Philipp Metzger, Pankaj Shende, Dhananjay Jere
Following intravitreal (IVT) injection, therapeutic proteins get exposed to physiological pH, temperature and components in the vitreous humor (VH) for a significantly long time. Therefore, it is of interest to study the stability of the proteins in the VH. However, the challenge posed by the isolated VH (such as pH shift upon isolation and incubation due to the formation of smaller molecular weight (MW) degradation products) can result in artefacts when investigating protein stability in relevance for the actual in vivo situation...
November 16, 2016: European Journal of Pharmaceutics and Biopharmaceutics
Diana P Gaspar, Carmen Serra, Paulo R Lino, Lídia Gonçalves, Pablo Taboada, Carmen Remuñán-López, António J Almeida
Associating protein with nanoparticles is an interesting strategy to improve their bioavailability and biological activity. Solid lipid nanoparticles (SLN) have been sought as carriers for therapeutic proteins transport to the lung epithelium. Nevertheless, because of their low inertia, nanoparticles intended for pulmonary application usually escape from lung deposition. To overcome this problem, the production of spray-dried powders containing nanoparticles has been recently reported. Herein we developed new hybrid microencapsulated SLN for pulmonary administration, containing a model protein (papain, PAP)...
January 10, 2017: International Journal of Pharmaceutics
Faranak Salmannejad, Nastaran Nafissi-Varcheh
This study is to investigate whether ectoines (ectoine and hydroxyectoine) can reduce aggregation of rhIFNα2b in aqueous solutions on thermal stress. The effect of thermal stress condition on the stability was therefore investigated using size exclusion-high performance liquid chromatography (SE-HPLC), different spectroscopic measurements, dynamic light scattering (DLS), electrophoresis, and differential scanning calorimetry (DSC). All experiments were performed in a sodium phosphate buffer system (100mM, pH7)...
January 15, 2017: European Journal of Pharmaceutical Sciences
Konstantin M Boyko, Tatiana V Rakitina, Dmitry A Korzhenevskiy, Anna V Vlaskina, Yuliya K Agapova, Dmitry E Kamashev, Sergey Y Kleymenov, Vladimir O Popov
The three-dimensional structure of the histone-like HU protein from the mycoplasma Spiroplasma melliferum KC3 (HUSpm) was determined at 1.4 Å resolution, and the thermal stability of the protein was evaluated by differential scanning calorimetry. A detailed analysis revealed that the three-dimensional structure of the HUSpm dimer is similar to that of its bacterial homologues but is characterized by stronger hydrophobic interactions at the dimer interface. This HUSpm dimer interface lacks salt bridges but is stabilized by a larger number of hydrogen bonds...
November 3, 2016: Scientific Reports
Shuyu Wang, Shifeng Yu, Michael S Siedler, Peter M Ihnat, Dana I Filoti, Ming Lu, Lei Zuo
We developed an ultrasensitive micro-DSC (differential scanning calorimeter) for liquid protein sample characterization. This design integrated vanadium oxide thermistors and flexible polymer substrates with microfluidics chambers to achieve a high sensitivity (6 V/W), low thermal conductivity (0.7 mW/K), high power resolutions (40 nW), and well-defined liquid volume (1 μl) calorimeter sensor in a compact and cost-effective way. We further demonstrated the performance of the sensor with lysozyme unfolding...
October 2016: Review of Scientific Instruments
Baiju P Krishnan, Rishika Rai, Aromal Asokan, Kana M Sureshan
Isosteric replacement of amide bond(s) of peptides with surrogate groups is an important strategy for the synthesis of peptidomimetics (pseudo-peptides). Triazole is a well-recognized bio-isostere for peptide bonds, and peptides with one or more triazole units are of great interest for different applications. We have used a catalyst-free and solvent-free method, viz., topochemical azide-alkyne cycloaddition (TAAC) reaction, to synthesize pseudo-proteins with repeating sequences. A designed β-sheet-forming l-Ala-l-Val dipeptide containing azide and alkyne at its termini (N3-Ala-Val-NHCH2C≡CH, 1) was synthesized...
November 16, 2016: Journal of the American Chemical Society
Vandana Dhaka, B S Khatkar
The gluten and its sub-fractions of good and poor bread quality wheat varieties were studied using scanning electron microscopy, differential scanning calorimetry (DSC) and IR spectroscopy techniques. The gluten of good bread quality wheat variety showed organized foam like matrix, whereas that of poor demonstrated an open gluten matrix. The glutenin of good bread quality wheat (HI 977) exhibited a more striated, organised texture in contrast to a dense, unorganised structure visible in C306. Gliadins of poor bread quality wheat were self-assembled to form a sheet like structure, whereas the gliadin proteins of good bread quality wheat variety showed more open microstructure...
August 2016: Journal of Food Science and Technology
Zhengrong Yang, Qingxian Zhou, Leo Mok, Anukriti Singh, Douglas J Swartz, Ina L Urbatsch, Christie G Brouillette
Structural changes in mouse P-glycoprotein (Pgp) induced by thermal unfolding were studied by differential scanning calorimetry (DSC), circular dichroism and fluorescence spectroscopy to gain insight into the solution conformation(s) of this ABC transporter that may not be apparent from current crystal structures. DSC of reconstituted Pgp showed two thermal unfolding transitions in the absence of MgATP, suggesting that each transition involved the cooperative unfolding of two or more interacting structural domains...
January 2017: Biochimica et Biophysica Acta
Pakkawat Detchewa, Masubon Thongngam, Jay-Lin Jane, Onanong Naivikul
The objective of this study was to investigate the effect of soy protein isolate on functional properties and consumer acceptance of gluten-free rice spaghetti (GFRS) made from rice flour. Dry-milled high-amylose (Chai Nat 1) rice flour was premixed with dry-milled waxy (RD 6) rice flour at a ratio of 90:10 (w/w) with the soy protein isolate (SPI) concentration varying between 0, 2.5, 5.0, 7.5, 10.0 %, db. The GFRS formulation was processed using a co-rotating twin-screw extruder up to 95 °C with a screw speed of 220 rpm, 32 % moisture content, and then dried at 40 °C...
September 2016: Journal of Food Science and Technology
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