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https://www.readbyqxmd.com/read/28931050/saxs-and-stability-studies-of-iron-induced-oligomers-of-bacterial-frataxin-cyay
#1
Mostafa Fekry, Wessen Alshokry, Przemysław Grela, Marek Tchórzewski, Eva-Christina Ahlgren, Christopher A Söderberg, Oleksandr Gakh, Grazia Isaya, Salam Al-Karadaghi
Frataxin is a highly conserved protein found in both prokaryotes and eukaryotes. It is involved in several central functions in cells, which include iron delivery to biochemical processes, such as heme synthesis, assembly of iron-sulfur clusters (ISC), storage of surplus iron in conditions of iron overload, and repair of ISC in aconitase. Frataxin from different organisms has been shown to undergo iron-dependent oligomerization. At least two different classes of oligomers, with different modes of oligomer packing and stabilization, have been identified...
2017: PloS One
https://www.readbyqxmd.com/read/28930630/assessing-the-structure-and-stability-of-transmembrane-oligomeric-intermediates-of-an-%C3%AE-helical-toxin
#2
Rajat Desikan, Prabal K K Maiti, K Ganapathy Ayappa
Protein membrane interactions play an important role in our understanding of diverse phenomena ranging from membrane assisted protein aggregation to oligomerization and folding. Pore forming toxins (PFTs) form the primary vehicle for infection by several strains of bacteria. These proteins expressed in a water soluble form (monomers) bind to the target membrane (protomer) to form a transmembrane pore complex through a process of oligomerization. Based on the structure of the transmembrane domains, PFTs are broadly classified into β or α toxins...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28930533/53bp1-a-guardian-for-centrosomal-integrity
#3
Haeyoung Kim, Hyungshin Yim
53BP1 is known as a mediator in DNA damage response and a regulator of DNA double-stranded breaks (DSBs) repair. 53BP1 was recently reported to be a centrosomal protein and a binding partner of mitotic polo-like kinase 1 (Plk1). The stability of 53BP1, in response to DSBs, is regulated by its phosphorylation, deubiquitination, and ubiquitination. During mitosis, 53BP1 is stabilized by phosphorylation at S380, a putative binding region with polo-box domain of Plk1, and deubiquitination by ubiquitin-specific protease 7 (USP7)...
January 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28930443/hydrogel-tethering-enhances-interdomain-stabilization-of-single-chain-antibodies
#4
Yijia Xiong, Nicole R Ford, Karen A Hecht, Guritno Roesijadi, Thomas C Squier
Here we identify the importance of molecular crowding agents in the functional stabilization of scFv antibodies. Antibodies were tethered through an engineered calmodulin (CaM) binding peptide into a stimulus-responsive hydrogel composed of poly-(ethylene glycol) (PEG) functionalized CaM. Macromolecular crowding is modulated by transient heating, which acts to decrease effective pore sizes. Using a fluorescent ligand bound to the scFv, frequency-domain fluorescence spectroscopy was used to assess the structural coupling between the VH and VL domains and relationships with functional stabilization...
September 20, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28930199/reduced-fluorescent-protein-switching-fatigue-by-binding-induced-emissive-state-stabilization
#5
Thijs Roebroek, Sam Duwé, Wim Vandenberg, Peter Dedecker
Reversibly switchable fluorescent proteins (RSFPs) enable advanced fluorescence imaging, though the performance of this imaging crucially depends on the properties of the labels. We report on the use of an existing small binding peptide, named Enhancer, to modulate the spectroscopic properties of the recently developed rsGreen series of RSFPs. Fusion constructs of Enhancer with rsGreen1 and rsGreenF revealed an increased molecular brightness and pH stability, although expression in living E. coli or HeLa cells resulted in a decrease of the overall emission...
September 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28930195/bioaccessibility-and-cellular-uptake-of-%C3%AE-carotene-encapsulated-in-model-o-w-emulsions-influence-of-initial-droplet-size-and-emulsifiers
#6
Wei Lu, Alan L Kelly, Song Miao
The effects of the initial emulsion structure (droplet size and emulsifier) on the properties of β-carotene-loaded emulsions and the bioavailability of β-carotene after passing through simulated gastrointestinal tract (GIT) digestion were investigated. Exposure to GIT significantly changed the droplet size, surface charge and composition of all emulsions, and these changes were dependent on their initial droplet size and the emulsifiers used. Whey protein isolate (WPI)-stabilized emulsion showed the highest β-carotene bioaccessibility, while sodium caseinate (SCN)-stabilized emulsion showed the highest cellular uptake of β-carotene...
September 20, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28929997/seasonal-variation-in-the-composition-and-processing-characteristics-of-herd-milk-with-varying-proportions-of-milk-from-spring-calving-and-autumn-calving-cows
#7
Yingchen Lin, James A O'Mahony, Alan L Kelly, Timothy P Guinee
The study investigated the seasonal changes in the compositional, physicochemical and processing characteristics of milk from a mixed-herd of spring- and autumn-calving cows during the year 2014-2015. The volume proportion of autumn-calving milk (% of total milk) varied with season, from ~10-20 in Spring (March-May), 5-13 in Summer (June-August), 20-40 in Autumn (September-November) and 50-100 in Winter (December-February). While all characteristics varied somewhat from month to month, variation was inconsistent, showing no significant trend with progression of time (year)...
September 20, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28929994/effect-of-chelators-on-functionality-of-milk-protein-concentrates-obtained-by-ultrafiltration-at-a-constant-ph-and-temperature
#8
Lata Ramchandran, XiaoXia Luo, Todor Vasiljevic
Modulating conditions during ultrafiltration of skim milk appears to be a feasible strategy to obtain milk protein concentrates (MPC) with tailored functionalities. Adjustment of pH and process temperature attenuated properties of casein micelle resulting in enhanced emulsification capacity. Additional pre-treatment options such as addition of calcium chelators can further impact on the functionality of MPC by modifying the calcium distribution and casein micelle integrity. The objective of the project was to establish effects of pre-treating skim milk with calcium chelators (EDTA or citrate) in concentrations between 10 to 30 mm prior to UF on the physical properties of the feed, corresponding retentates and dried MPC, including particle size, zeta potential and calcium distribution in skim milk and the corresponding retentates, as well as the physical functionalities such as solubility, heat stability and emulsifying properties...
September 20, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28929622/endonuclease-regnase-1-monocyte-chemotactic-protein-1-induced-protein-1-mcpip1-in-controlling-immune-responses-and-beyond
#9
REVIEW
Osamu Takeuchi
The activation of inflammatory cells is controlled at transcriptional and posttranscriptional levels. Posttranscriptional regulation modifies mRNA stability and translation, allowing for elaborate control of proteins required for inflammation, such as proinflammatory cytokines, prostaglandin synthases, cell surface co-stimulatory molecules, and even transcriptional modifiers. Such regulation is important for coordinating the initiation and resolution of inflammation, and is mediated by a set of RNA-binding proteins (RBPs), including Regnase-1, Roquin, Tristetraprolin (TTP), and AU-rich elements/poly(U)-binding/degradation factor 1 (AUF1)...
September 20, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/28929266/brazilian-meningococcal-c-conjugate-vaccine-physicochemical-immunological-and-thermal-stability-characteristics
#10
Renata Chagas Bastos, Marilza Batista Corrêa, Iaralice Medeiros de Souza, Milton Neto da Silva, Denise da Silva Gomes Pereira, Fernanda Otaviano Martins, Camila da Silva Faria, Ana Paula Dinis Ano Bom, Maria de Lourdes Leal, Ellen Jessouroun, José Godinho da Silva, Ricardo de Andrade Medronho, Ivna Alana Freitas Brasileiro da Silveira
High temperature is known to cause some instability in polysaccharide-protein conjugated vaccines and studies under stress conditions may be useful in determining whether short-term accidental exposure to undesired conditions can compromise product quality. In this study, we examined the structural stability of three industrial batches of Brazilian Meningococcal C conjugate bulk (MPCT) incubated at 4, 37, and 55 °C for 5 weeks. The effect of exposure to the storage temperatures was monitored by HPLC-SEC, CZE, CD and NMR techniques...
September 19, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28928761/hormonal-regulation-in-shade-avoidance
#11
REVIEW
Chuanwei Yang, Lin Li
At high vegetation density, shade-intolerant plants sense a reduction in the red (660 nm) to far-red (730 nm) light ratio (R/FR) in addition to a general reduction in light intensity. These light signals trigger a spectrum of morphological changes manifested by growth of stem-like tissue (hypocotyl, petiole, etc.) instead of harvestable organs (leaves, fruits, seeds, etc.)-namely, shade avoidance syndrome (SAS). Common phenotypical changes related to SAS are changes in leaf hyponasty, an increase in hypocotyl and internode elongation and extended petioles...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28928732/facile-affinity-maturation-of-antibody-variable-domains-using-natural-diversity-mutagenesis
#12
Kathryn E Tiller, Ratul Chowdhury, Tong Li, Seth D Ludwig, Sabyasachi Sen, Costas D Maranas, Peter M Tessier
The identification of mutations that enhance antibody affinity while maintaining high antibody specificity and stability is a time-consuming and laborious process. Here, we report an efficient methodology for systematically and rapidly enhancing the affinity of antibody variable domains while maximizing specificity and stability using novel synthetic antibody libraries. Our approach first uses computational and experimental alanine scanning mutagenesis to identify sites in the complementarity-determining regions (CDRs) that are permissive to mutagenesis while maintaining antigen binding...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28928627/a-novel-truncation-mutation-in-crybb1-associated-with-autosomal-dominant-congenital-cataract-with-nystagmus
#13
Yan Rao, Sufang Dong, Zuhua Li, Guohua Yang, Chunyan Peng, Ming Yan, Fang Zheng
PURPOSE: To identify the potential candidate genes for a large Chinese family with autosomal dominant congenital cataract (ADCC) and nystagmus, and investigate the possible molecular mechanism underlying the role of the candidate genes in cataractogenesis. METHODS: We combined the linkage analysis and direct sequencing for the candidate genes in the linkage regions to identify the causative mutation. The molecular and bio-functional properties of the proteins encoded by the candidate genes was further explored with biophysical and biochemical studies of the recombinant wild-type and mutant proteins...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28928523/influence-of-ph-and-salt-concentration-on-functional-properties-of-walnut-protein-from-different-extraction-methods
#14
Haifang Hu, Tao Fan, Xiaoyan Zhao, Xiaowei Zhang, Yali Sun, Hongkai Liu
The effect of pH and NaCl on solubility and functional properties of walnut proteins obtained through AOT reverse micelles, enzyme-assisted reverse micelles and aqueous phase extraction methods was investigated and compared. Extraction yield, foaming properties, water holding capacities of protein obtained through enzyme-assisted reverse micelles at pH 2-12 and NaCl concentration 0.1-1 M were significantly higher than those of the AOT reverse micelles and aqueous phase extracted two. The solubility of proteins by AOT reverse micelles and enzyme-assisted reverse micelles at certain pH and NaCl concentration had no significant difference, but was higher that of the aqueous buffer...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28928489/the-phosphorylation-of-a-kinetochore-protein-dam1-by-aurora-b-ipl1-kinase-promotes-chromosome-bipolar-attachment-in-yeast
#15
Fengzhi Jin, Michael Bokros, Yanchang Wang
The interaction between chromosomes and spindle microtubules is essential for chromosome segregation. The kinetochore complex mediates this interaction. Previous studies indicate that the stability of kinetochore attachment is regulated by Aurora B/Ipl1 kinase and this regulation is conserved from yeast to mammalian cells. In budding yeast Saccharomyces cerevisiae, the ten-subunit Dam1/DASH complex bridges the interaction between kinetochores and microtubules, and some in vitro evidence indicates that the phosphorylation of Dam1 protein by Ipl1 kinase destabilizes this interaction...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28928443/affected-energy-metabolism-under-manganese-stress-governs-cellular-toxicity
#16
Gursharan Kaur, Vineet Kumar, Amit Arora, Ajay Tomar, Ashish, Runa Sur, Dipak Dutta
Excessive manganese exposure is toxic, but a comprehensive biochemical picture of this assault is poorly understood. Whether oxidative stress or reduced energy metabolism under manganese exposure causes toxicity is still a debate. To address this, we chose Δmnt P Escherichia coli, a highly manganese-sensitive strain, in this study. Combining microarray, proteomics, and biochemical analyses, we show that the chronic manganese exposure rewires diverse regulatory and metabolic pathways. Manganese stress affects protein and other macromolecular stability, and envelope biogenesis...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28928438/zyxin-stabilizes-rig-i-and-mavs-interactions-and-promotes-type-i-interferon-response
#17
Takahisa Kouwaki, Masaaki Okamoto, Hirotake Tsukamoto, Yoshimi Fukushima, Misako Matsumoto, Tsukasa Seya, Hiroyuki Oshiumi
RIG-I and MDA5 are cytoplasmic viral RNA sensors that belong to the RIG-I-like receptors (RLRs), which induce antiviral innate immune responses, including the production of type I interferon and other pro-inflammatory cytokines. After recognition of viral RNA, the N-terminal caspase activation and recruitment domains (CARDs) of RIG-I and MDA5 bind to a CARD in the MAVS adaptor molecule, resulting in MAVS oligomerization and downstream signaling. To reveal the molecular mechanism of MAVS-dependent signaling, we performed a yeast two-hybrid screening and identified zyxin as a protein that binds to MAVS...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28928239/the-rna-editing-enzyme-adar-promotes-lung-adenocarcinoma-migration-and-invasion-by-stabilizing-fak
#18
Elianna M Amin, Yuan Liu, Su Deng, Kay See Tan, Neel Chudgar, Marty W Mayo, Francisco Sanchez-Vega, Prasad S Adusumilli, Nikolaus Schultz, David R Jones
Large-scale, genome-wide studies report that RNA binding proteins are altered in cancers, but it is unclear how these proteins control tumor progression. We found that the RNA-editing protein ADAR (adenosine deaminase acting on double-stranded RNA) acted as a facilitator of lung adenocarcinoma (LUAD) progression through its ability to stabilize transcripts encoding focal adhesion kinase (FAK). In samples from 802 stage I LUAD patients, increased abundance of ADAR at both the mRNA and protein level correlated with tumor recurrence...
September 19, 2017: Science Signaling
https://www.readbyqxmd.com/read/28928145/structure-insight-of-gsdmd-reveals-the-basis-of-gsdmd-autoinhibition-in-cell-pyroptosis
#19
Siyun Kuang, Jun Zheng, Hui Yang, Suhua Li, Shuyan Duan, Yanfang Shen, Chaoneng Ji, Jianhua Gan, Xue-Wei Xu, Jixi Li
Recent findings have revealed that the protein gasdermin D (GSDMD) plays key roles in cell pyroptosis. GSDMD binds lipids and forms pore structures to induce pyroptosis upon microbial infection and associated danger signals. However, detailed structural information for GSDMD remains unknown. Here, we report the crystal structure of the C-terminal domain of human GSDMD (GSDMD-C) at 2.64-Å resolution. The first loop on GSDMD-C inserts into the N-terminal domain (GSDMD-N), which helps stabilize the conformation of the full-length GSDMD...
September 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28928125/ck2%C3%AE-critically-regulates-thrombopoiesis-and-ca-2-triggered-platelet-activation-in-arterial-thrombosis-in-vivo
#20
Patrick Münzer, Britta Walker-Allgaier, Sascha Geue, Friederike Langhauser, Eva Geuss, David Stegner, Katja Aurbach, Daniela Semeniak, Madhumita Chatterjee, Irene Gonzalez Menendez, Melanie Märklin, Leticia Quintanilla-Martinez, Helmut R Salih, David W Litchfield, Thierry Buchou, Christoph Kleinschnitz, Florian Lang, Bernhard Nieswandt, Irina Pleines, Harald Schulze, Meinrad Gawaz, Oliver Borst
Platelets, anucleated megakaryocyte-derived cells, play a major role in hemostasis and arterial thrombosis. While protein kinase Casein Kinase 2 (CK2) is readily detected in megakaryocytes and platelets, the impact of CK2-dependent signaling on megakaryocyte/platelet (patho-) physiology has remained elusive. The present study explored the impact of the CK2 regulatory β-subunit on platelet biogenesis and activation. Megakaryocyte/platelet-specific genetic deletion of CK2β (ck2β(-/-) ) in mice resulted in a significant macrothrombocytopenia and an increased extramedullar megakaryopoiesis with enhanced proportion of premature platelets...
September 19, 2017: Blood
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