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https://www.readbyqxmd.com/read/28110232/physical-properties-and-extracellular-polymeric-substances-pattern-of-aerobic-granular-sludge-treating-hypersaline-wastewater
#1
Santo Fabio Corsino, Marco Capodici, Michele Torregrossa, Gaspare Viviani
The modification of the physical properties of aerobic granular sludge treating fish-canning wastewater is discussed in this paper. The structure and composition of the Extracellular Polymeric Substances (EPSs) were analyzed at different salinity levels and related to granules stability. Results outlined that the total EPSs content increased with salinity, despite the EPSs increment was not proportional to the salt concentration. Moreover, the EPSs structure was significantly modified by salinity, leading to a gradual increase of the not-bound EPSs fraction, which was close to the 50% of the total EPSs content at 75gNaClL(-1)...
January 13, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28110183/noncovalent-interaction-assisted-fullerene-for-the-transportation-of-some-brain-anticancer-drugs-a-theoretical-study
#2
Pabitra Narayan Samanta, Kalyan Kumar Das
The treatment of brain cancer like glioblastoma multiforme often uses chemotherapeutic drugs like temozolomide, procarbazine, carmustine, and lomustine. Fullerene loaded with these drugs help to cross the blood brain barriers. The adsorptions of the four drug molecules on the surface of the fullerene are studied mostly by using density functional theory (DFT) based method at the M06-2X/6-31G(d) level of calculations. In all four cases, the estimated interactions are noncovalent type and the average adsorption energy lies in between -5 and -11kcal/mol in the gas phase...
January 8, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28109840/isoform-specific-phosphorylation-in-human-hsp90%C3%AE-affects-interaction-with-clients-and-the-cochaperone-cdc37
#3
Minh T N Nguyen, Robert A Knieß, Soumya Daturpalli, Laura Le Breton, Xiangyu Ke, Xuemei Chen, Matthias P Mayer
Hsp90s assist maturation of many key regulators of signal transduction pathways and cellular control circuits like protein kinases and transcription factors and chaperone their stability and activity. In this function Hsp90s cooperate with some 30 cochaperones and they are themselves subject to regulation by numerous posttranslational modifications. In vertebrates two major isoforms exist in the cytosol, Hsp90α and Hsp90β, which share a high degree of sequence identity and are expressed in tissue- and environmental conditions-dependent manner...
January 18, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28109807/the-role-of-local-residue-environmental-changes-in-thermostable-mutants-of-the-gh11-xylanase-from-bacillus-subtilis
#4
Sérgio Ruschi Bergamachi Silva, Matheus Pinto Pinheiro, Carlos Alessandro Fuzo, Samuel Reghim Silva, Tatiane Lopes Ferreira, Marcos Roberto Lourenzoni, M Cristina Nonato, Davi Serradella Vieira, Richard John Ward
A thermostable variant of the mesophilic xylanase A from Bacillus subtilis (BsXynA-G3_4x) contains the four mutations Gln7His, Gly13Arg, Ser22Pro, and Ser179Cys. The crystal structure of the BsXynA-G3_4x has been solved, and the local environments around each of these positions investigated by molecular dynamics (MD) simulations at 328K and 348K. The structural and MD simulation results were correlated with thermodynamic data of the wild-type enzyme, the 4 single mutants and the BsXynA-G3_4x. This analysis suggests that the overall stabilizing effect is entropic, and is consistent with solvation of charged residues and reduction of main-chain flexibility...
January 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28109677/effect-of-pulsed-ultrasound-on-the-physicochemical-characteristics-and-emulsifying-properties-of-squid-dosidicus-gigas-mantle-proteins
#5
O A Higuera-Barraza, W Torres-Arreola, J M Ezquerra-Brauer, F J Cinco-Moroyoqui, J C Rodríguez Figueroa, E Marquez-Ríos
Food technologists are always looking to improve the functional properties of proteins. In this sense, in last years ultrasound has been used to improve some functional properties. For this reason, and considering that jumbo squid is an important fishery in northwest Mexico, the purpose of this research was to determine the effect of pulsed ultrasound on the physicochemical characteristics and emulsifying properties of squid (Dosidicus gigas) mantle proteins. Pulsed ultrasound (20kHz, 20, and 40% amplitude) was applied for 30, 60, and 90s to a protein extract prepared from giant squid mantle causing an increase (p<0...
January 12, 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28109637/congenital-mirror-movements-in-a-patient-with-alpha-dystroglycanopathy-due-to-a-novel-pomk-mutation
#6
Didem Ardicli, Rahsan Gocmen, Beril Talim, Rosanne Sprute, Goknur Haliloglu, Sebahattin Cirak, Haluk Topaloglu
Dystroglycanopathies are a heterogeneous group of muscular dystrophies often associated with variable brain and eye involvement. Glycosylated alpha-dystroglycan (ADG) plays a key role in the development and stability of basement membranes as well as organizing axon guidance in the central nervous system. Congenital mirror movements, either isolated or in association with several genetic syndromes, are defined as inability to perform unimanual movements. We report an adolescent boy with limb-girdle muscular dystrophy due to ADG deficiency and coexisting congenital mirror movements...
December 23, 2016: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/28109561/lithium-reverses-behavioral-and-axonal-transport-related-changes-associated-with-ank3-bipolar-disorder-gene-disruption
#7
Michael G Gottschalk, Melanie P Leussis, Tillmann Ruland, Klaudio Gjeluci, Tracey L Petryshen, Sabine Bahn
Ankyrin 3 (ANK3) has been implicated as a genetic risk factor for bipolar disorder (BD), however the resulting pathophysiological and treatment implications remain elusive. In a preclinical systems biological approach, we aimed to characterize the behavioral and proteomic effects of Ank3 haploinsufficiency and chronic mood-stabilizer treatment in mice. Psychiatric-related behavior was evaluated with the novelty-suppressed feeding (NSF) paradigm, elevated plus maze (EPM) and a passive avoidance task (PAT). Tandem mass spectrometry (MS(E)) was employed for hippocampal proteome profiling...
January 18, 2017: European Neuropsychopharmacology: the Journal of the European College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28109441/quantitative-glycomics-a-combined-analytical-and-bioinformatics-approach
#8
L Veillon, S Zhou, Y Mechref
Glycosylation is one of the most common and essential protein modifications. Glycans conjugated to biomolecules modulate the function of such molecules through both direct recognition of glycan structures and indirect mechanisms that involve the control of protein turnover rates, stability, and conformation. The biological attributes of glycans in numerous biological processes and implications in a number of diseases highlight the necessity for comprehensive characterization of protein glycosylation. This chapter reviews cutting-edge methods and tools developed to facilitate quantitative glycomics...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28109331/protein-aggregation-and-molecular-crowding-perspectives-from-multiscale-simulations
#9
F Musiani, A Giorgetti
Cells are extremely crowded environments, thus the use of diluted salted aqueous solutions containing a single protein is too simplistic to mimic the real situation. Macromolecular crowding might affect protein structure, folding, shape, conformational stability, binding of small molecules, enzymatic activity, interactions with cognate biomolecules, and pathological aggregation. The latter phenomenon typically leads to the formation of amyloid fibrils that are linked to several lethal neurodegenerative diseases, but that can also play a functional role in certain organisms...
2017: International Review of Cell and Molecular Biology
https://www.readbyqxmd.com/read/28109327/effects-of-intrinsic-and-extrinsic-factors-on-aggregation-of-physiologically-important-intrinsically-disordered-proteins
#10
L Breydo, J M Redington, V N Uversky
Misfolding and aggregation of proteins and peptides play an important role in a number of diseases as well as in many physiological processes. Many of the proteins that misfold and aggregate in vivo are intrinsically disordered. Protein aggregation is a complex multistep process, and aggregates can significantly differ in morphology, structure, stability, cytotoxicity, and self-propagation ability. The aggregation process is influenced by both intrinsic (e.g., mutations and expression levels) and extrinsic (e...
2017: International Review of Cell and Molecular Biology
https://www.readbyqxmd.com/read/28109185/folding-of-intrinsically-disordered-plant-lea-proteins-is-driven-by-glycerol-induced-crowding-and-the-presence-of-membranes
#11
Anne Bremer, Martin Wolff, Anja Thalhammer, Dirk K Hincha
Late embryogenesis abundant (LEA) proteins are related to cellular dehydration tolerance. Most LEA proteins are predicted to have no stable secondary structure in solution, i.e. to be intrinsically disordered proteins (IDPs), but they may acquire α-helical structure upon drying. In the model plant Arabidopsis thaliana the LEA proteins COR15A and COR15B are highly induced upon cold treatment and are necessary for the plants to attain full freezing tolerance. Freezing leads to increased intracellular crowding due to dehydration by extracellular ice crystals...
January 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28108661/a-structure-function-analysis-of-the-yeast-elg1-protein-reveals-the-importance-of-pcna-unloading-in-genome-stability-maintenance
#12
Keren Shemesh, Marek Sebesta, Martin Pacesa, Soumitra Sau, Alex Bronstein, Oren Parnas, Batia Liefshitz, Česlovas Venclovas, Lumir Krejci, Martin Kupiec
The sliding clamp, PCNA, plays a central role in DNA replication and repair. In the moving replication fork, PCNA is present at the leading strand and at each of the Okazaki fragments that are formed on the lagging strand. PCNA enhances the processivity of the replicative polymerases and provides a landing platform for other proteins and enzymes. The loading of the clamp onto DNA is performed by the Replication Factor C (RFC) complex, whereas its unloading can be carried out by an RFC-like complex containing Elg1...
January 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28108589/chromatin-regulation-by-the-nua4-acetyltransferase-complex-is-mediated-by-essential-interactions-between-enhancer-of-polycomb-epl1-and-esa1
#13
Naomi E Searle, Ana Lilia Torres-Machorro, Lorraine Pillus
Enzymes that modify and remodel chromatin act in broadly conserved macromolecular complexes. One key modification is the dynamic acetylation of histones and other chromatin proteins by opposing activities of acetyltransferase and deacetylase complexes. Among acetyltransferases, the NuA4 complex containing Tip60 or its Saccharomyces cerevisiae ortholog, Esa1, is of particular significance because of its roles in crucial genomic processes including DNA damage repair and transcription. The catalytic subunit Esa1 is essential, as are five non-catalytic NuA4 subunits...
January 20, 2017: Genetics
https://www.readbyqxmd.com/read/28108349/expression-of-a-soluble-truncated-vargula-luciferase-in-escherichia-coli
#14
Eric A Hunt, Angeliki Moutsiopoulou, David Broyles, Trajen Head, Emre Dikici, Sylvia Daunert, Sapna K Deo
Marine luciferases are regularly employed as useful reporter molecules across a range of various applications. However, attempts to transition expression from their native eukaryotic environment into a more economical prokaryotic, i.e. bacterial, expression system often presents several challenges. Specifically, bacterial protein expression inherently lacks chaperone proteins to aid in the folding process, while Escherichia coli presents a reducing cytoplasmic environment in. These conditions contribute to the inhibition of proper folding of cysteine-rich proteins, leading to incorrect tertiary structure and ultimately inactive and potentially insoluble protein...
January 17, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28108341/molecular-cloning-genomic-structure-polymorphism-analysis-and-recombinant-expression-of-a-%C3%AE-1-antitrypsin-like-gene-from-swamp-eel-monopterus-albus
#15
Wei Li, Quanhe Wang, Shaobin Li, Ao Jiang, Wenxiu Sun
Alpha-1-antitrypsin (AAT) is a highly polymorphic glycoprotein antiprotease, involved in the regulation of human immune response. Beyond some genomic characterization and a few protein characterizations, the function of teleost AAT remains uncertain. In this study we cloned an AAT-like gene from a swamp eel liver identifying four exons and three introns, and the full-length cDNA. The elucidated swamp eel AAT amino acid sequence showed high homology with known AATs from other teleosts. The swamp eel AAT was examined both in ten healthy tissues and in four bacterially-stimulated tissues resulting in up-regulation of swamp eel AAT at different times...
January 17, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28108276/hydrogen-sulfide-reduces-rage-toxicity-through-inhibition-of-its-dimer-formation
#16
Hong Zhou, Lei Ding, Zhiyuan Wu, Xu Cao, Qichun Zhang, Li Lin, Jin-Song Bian
RAGE is important in the development of neurodegenerative diseases. The present study was designed to investigate the effect of hydrogen sulfide (H2S, an endogenous gaseous mediator) on the cytotoxicity caused by RAGE activation during the chronic oxidative stress. Aβ1-42 decreased cell viability and induced cell senescence in SH-SY5Y cells. Treatment with advanced glycation end products (AGEs) induced cell injury in HEK293 cells stably expressing RAGE (HEK293-RAGE) and stimulated inflammatory responses in SH-SY5Y cells...
January 17, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28108249/synthesis-and-biological-evaluation-of-thiazole-derivatives-as-novel-usp7-inhibitors
#17
Chao Chen, Jiemei Song, Jinzheng Wang, Chang Xu, Caiping Chen, Wei Gu, Hongbin Sun, Xiaoan Wen
Herpesvirus-associated Ubiquitin-Specific Protease (HAUSP, also called USP7) interacts with and stabilizes Mdm2, and represents one of the first examples that deubiquitinases oncogenic proteins. USP7 has been regarded as a potential drug target for cancer therapy. Inhibitors of USP7 have been recently shown to suppress tumor cell growth in vitro and in vivo. Based on leading USP7 inhibitors P5091 and P22077, we designed and synthesized a series of thiazole derivatives. The results of in vitro assays showed that the thiazole compounds exhibited low micromolar inhibition activity against both USP7 enzyme and cancer cell lines...
January 10, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28107962/a-label-free-electrochemical-immunosensor-based-on-a-new-polymer-containing-aldehyde-and-ferrocene-groups
#18
Xiangyang Zhang, Youming Shen, Youyu Zhang, Guangyu Shen, Haiyan Xiang, Xiaofeng Long
In this work, a novel and convenient strategy for synthesis of a well-defined polymer containing both aldehyde and ferrocene functional groups by grafting 4-hydroxybenzaldehyde and ferrocenecarboxylhydrazide onto linear poly (vinylbenzyl chloride) was developed. The designed polymer film (polymer CAF) could be used as a new support matrix for fabrication of a label-free electrochemical immunosensor with high sensitivity, good stability and repeatability. With human IgG as an analyte model, the proposed electrochemical immunosensor showed a linear concentration range from 0...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107835/one-step-synthesis-of-dna-functionalized-cadmium-free-quantum-dots-and-its-application-in-fret-based-protein-sensing
#19
Cuiling Zhang, Caiping Ding, Guohua Zhou, Qin Xue, Yuezhong Xian
DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn(2+)) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay...
March 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28107768/arabidopsis-protein-s-acyl-transferase4-mediates-root-hair-growth
#20
Zhi-Yuan Wan, Sen Chai, Fu-Rong Ge, Qiang-Nan Feng, Yan Zhang, Sha Li
Polar growth of root hairs is critical for plant survival and necessitates fine-tuned ROP signaling. Multiple ROP regulators participate in root hair growth. However, protein S-acyl transferases (PATs), mediating the S-acylation and membrane partitioning of ROPs, are yet to be found. By using a reverse genetic approach, combining fluorescence probes, pharmacological drugs, site-directed mutagenesis, and genetic analysis with related root-hair mutants, we have identified and characterized an Arabidopsis PAT, which may be responsible for ROP2 S-acylation in root hairs...
January 20, 2017: Plant Journal: for Cell and Molecular Biology
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