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https://www.readbyqxmd.com/read/28340387/an-exploration-of-the-effect-and-interaction-mechanism-of-bisphenol-a-on-waste-sludge-hydrolysis-with-multi-spectra-isothermal-titration-microcalorimetry-and-molecule-docking
#1
Guangying Hou, Rui Zhang, Xiaoyan Hao, Chunguang Liu
An increasing amount of bisphenol A (BPA) is being produced and used, then discharged into sewage treatment plants and accumulated in sludge or soil, when the sludge is used as fertilizer. Accumulation of BPA in sludge or soil causes poisoning to the enzyme, which affects the biological treatment of sludge and the circulation and conversion of materials in soil. In this research, effect of BPA on sludge hydrolysis is studied from the respect of concentration and components of soluble organic matter in sludge, using three-dimensional fluorescence spectra...
March 10, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28340053/does-the-regulatory-environment-for-e-cigarettes-influence-the-effectiveness-of-e-cigarettes-for-smoking-cessation-longitudinal-findings-from-the-itc-four-country-survey
#2
Hua-Hie Yong, Sara C Hitchman, K Michael Cummings, Ron Borland, Shannon M L Gravely, Ann McNeill, Geoffrey T Fong
Introduction: To date, no studies have explored how different regulatory environments may influence the effectiveness of ECs as a smoking cessation aid. Objective: This study compares the real-world effectiveness of adult smokers using ECs for quitting compared with quitting unassisted or quitting with NRT and/or prescription medications in two countries with restrictive policies towards ECs (i.e., Canada and Australia) versus two countries with less restrictive policies (i...
March 4, 2017: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
https://www.readbyqxmd.com/read/28334770/smokers-perceptions-of-sources-of-advice-about-quitting-findings-from-the-australian-arm-of-the-itc-4-country-survey
#3
James Balmford, Ron Borland
Smokers are exposed to advice about quitting from numerous sources. Within the 2013 ITC 4-Country Survey, 1211 Australian smokers or recent ex-smokers rated the perceived importance of eight sources of advice, categorized into evidence-based, non evidence-based, personal experience and vicarious experience (two items each), and also rated their intention to quit, nicotine dependence, use of quit medication, health concerns and harm beliefs. The eight items were all positively correlated. Respondents who placed greater importance on their experiences (either personal or vicarious) were more likely to agree that the evidence for smoking-related harm is exaggerated, and although not more likely to intend to quit overall, these responses were most strongly related to quit intention...
February 27, 2017: Health Education Research
https://www.readbyqxmd.com/read/28330825/streamlining-workflow-and-automation-to-accelerate-laboratory-scale-protein-production
#4
REVIEW
Jennifer Konczal, Christopher H Gray
Protein production facilities are often required to produce diverse arrays of proteins for demanding methodologies including crystallography, NMR, ITC and other reagent intensive techniques. It is common for these teams to find themselves a bottleneck in the pipeline of ambitious projects. This pressure to deliver has resulted in the evolution of many novel methods to increase capacity and throughput at all stages in the pipeline for generation of recombinant proteins. This review aims to describe current and emerging options to accelerate the success of protein production in Escherichia coli...
March 18, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28327612/endogenous-opioids-regulate-moment-to-moment-neuronal-communication-and-excitability
#5
Bryony L Winters, Gabrielle C Gregoriou, Sarah A Kissiwaa, Oliver A Wells, Danashi I Medagoda, Sam M Hermes, Neil T Burford, Andrew Alt, Sue A Aicher, Elena E Bagley
Fear and emotional learning are modulated by endogenous opioids but the cellular basis for this is unknown. The intercalated cells (ITCs) gate amygdala output and thus regulate the fear response. Here we find endogenous opioids are released by synaptic stimulation to act via two distinct mechanisms within the main ITC cluster. Endogenously released opioids inhibit glutamate release through the δ-opioid receptor (DOR), an effect potentiated by a DOR-positive allosteric modulator. Postsynaptically, the opioids activate a potassium conductance through the μ-opioid receptor (MOR), suggesting for the first time that endogenously released opioids directly regulate neuronal excitability...
March 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28326635/structure-enabled-discovery-of-a-stapled-peptide-inhibitor-to-target-the-oncogenic-transcriptional-repressor-tle1
#6
Sally McGrath, Marcello Tortorici, Ludovic Drouin, Savade Solanki, Lewis Vidler, Isaac Westwood, Peter Gimeson, Rob vanMontfort, Swen Hoelder
TLE1 is an oncogenic transcriptional co-repressor that exerts its repressive effects through binding of transcription factors. Inhibition of this protein-protein interaction represents a putative cancer target but no small molecule inhibitors have been published for this challenging interface. In this manuscript, we report the structure enabled design and synthesis of a constrained peptide inhibitor of TLE1. Our design featured introduction of a four carbon atom linker into the peptide epitope found in many TLE1 binding partners...
March 22, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28317952/selective-perrhenate-recognition-in-pure-water-by-halogen-bonding-and-hydrogen-bonding-alpha-cyclodextrin-based-receptors
#7
Stuart P Cornes, Mark R Sambrook, Paul D Beer
Alpha-cyclodextrin based anion receptors functionalised with pendant arms containing halogen and hydrogen bond donor motifs display selective association of perrhenate in aqueous media at neutral pH. NMR and ITC anion binding investigations reveal the halogen bonding receptor to be the superior host.
March 20, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28302225/-advances-in-research-of-antitumor-mechanisms-of-isothiocyanates
#8
Huimin Wang, Ke Xu
Isothiocyanates (ITCs) are naturally occurring small molecules that are generated by the enzymic hydrolysis of glucosinolate in cruciferous vegetables. Numerous studies showed that ITCs inhibit the growth of tumors by the mechanisms including inducing cell cycle arrest, promoting apoptosis and producing reactive oxygen species in vitro and in vivo. Recent studies showed that ITCs also inhibit metastasis of cancer cells, induce endoplasmic reticulum stress and autophagy. This review summarizes the antitumor mechanisms of ITCs...
March 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28294636/indirect-treatment-comparison-of-two-non-invasive-patient-controlled-analgesia-treatments-for-acute-post-operative-pain-management
#9
Pablo Katz, Shweta Takyar, Pamela Palmer, Hiltrud Liedgens
OBJECTIVES: To evaluate the relative clinical efficacy, safety, and tolerability associated with two non-invasive patient-controlled analgesia (PCA) treatments, sufentanil sublingual tablet system (SSTS) and fentanyl iontophoretic patient-controlled transdermal system (PCTS). These two treatments have recently been approved in the EU for the management of acute moderate-to-severe post-operative pain in adult patients. METHODS: As no head-to-head trials comparing SSTS and PCTS currently exist, indirect treatment comparison (ITC) analyses were conducted to evaluate SSTS or PCTS versus intravenous (IV) morphine PCA...
March 15, 2017: Current Medical Research and Opinion
https://www.readbyqxmd.com/read/28287519/endobronchial-ultrasound-guided-intratumoral-injection-of-cisplatin-for-the-treatment-of-isolated-mediastinal-recurrence-of-lung-cancer
#10
Hiren J Mehta, Michael A Jantz
Isolated hilar and mediastinal recurrence (IMHR) following external beam radiation therapy (EBRT) in patients with lung cancer is common. These patients do not have many treatment options and are usually offered palliative chemotherapy or best supportive care. Endobronchial ultrasound (EBUS)-guided intratumoral injection of cisplatin (ITC) is a novel approach for these patients. The procedure is performed under conscious sedation. The lesion is located with a bronchoscopy using EBUS, and a 22-gauge EBUS needle is advanced through the working channel of the scope and locked in position...
February 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28286323/switchsense-a-new-technology-to-study-protein-rna-interactions
#11
Antoine Cléry, Thibault J M Sohier, Thomas Welte, Andreas Langer, Frédéric H T Allain
Characterization of RNA-binding protein interactions with RNA became inevitable to properly understand the cellular mechanisms involved in gene expression regulation. Structural investigations bring information at the atomic level on these interactions and complementary methods such as Isothermal Titration Calorimetry (ITC) and Surface Plasmon Resonance (SPR) are commonly used to quantify the affinity of these RNA-protein complexes and evaluate the effect of mutations affecting these interactions. The switchSENSE technology has recently been developed and already successfully used to investigate protein interactions with different types of binding partners (DNA, protein/peptide or even small molecules)...
March 9, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28276693/biopolymer-targeted-adsorption-onto-halloysite-nanotubes-in-aqueous-media
#12
Vanessa Bertolino, Giuseppe Cavallaro, Giuseppe Lazzara, Stefana Milioto, Filippo Parisi
Studies on the adsorption of biopolymers onto halloysite nanotubes (HNTs) in water were conducted. Three polymers with different charges-anionic (pectin), neutral (hydroxypropyl cellulose), and cationic (chitosan)-were chosen. The thermodynamic parameters for the adsorption of polymers onto the HNT surface were determined by isothermal titration calorimetry (ITC). The experimental data were interpreted based on a Langmuir adsorption model. The standard variations in free energy, enthalpy, and entropy of the process were obtained and discussed...
March 21, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28276274/multi-spectroscopic-and-molecular-modelling-approach-to-investigate-the-interaction-of-riboflavin-with-human-serum-albumin
#13
Md Maroof Alam, Faizan Abul Qais, Iqbal Ahmad, Parvez Alam, Rizwan Hasan Khan, Imrana Naseem
Riboflavin (RF) plays an important role in various metabolic redox reactions in the form of flavin adenine dinucleotide and flavin mononucleotide. Human serum albumin (HSA) is an important protein involved in the transportation of drugs, hormones, fatty acid and other molecules which determine the biodistribution and physiological fate of these molecules. In this study, we have investigated the interaction of riboflavin RF with HSA under simulative physiological conditions using various biophysical, calorimetric and molecular docking techniques...
March 9, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28273138/itc-derived-binding-affinity-may-be-biased-due-to-titrant-nano-aggregation-binding-of-halogenated-benzotriazoles-to-the-catalytic-domain-of-human-protein-kinase-ck2
#14
Maria Winiewska, Ewa Bugajska, Jarosław Poznański
The binding of four bromobenzotriazoles to the catalytic subunit of human protein kinase CK2 was assessed by two complementary methods: Microscale Thermophoresis (MST) and Isothermal Titration Calorimetry (ITC). New algorithm proposed for the global analysis of MST pseudo-titration data enabled reliable determination of binding affinities for two distinct sites, a relatively strong one with the Kd of the order of 100 nM and a substantially weaker one (Kd > 1 μM). The affinities for the strong binding site determined for the same protein-ligand systems using ITC were in most cases approximately 10-fold underestimated...
2017: PloS One
https://www.readbyqxmd.com/read/28270074/molecular-and-thermodynamic-studies-on-dna-triplex-formed-in-the-promoter-region-of-hmgb1-gene-as-a-selective-target-for-anticancer-drugs
#15
Neelam Lohani, Rajeswari Raja Moganty
HMGB1 (High Mobility Group Box-1) is a very versatile highly abundant architectural protein that plays multiple roles in human health and diseases. Under physiological condition it serves an amazing assortment of roles in different compartments of cell. The reported high expression of HMGB1 in almost all types of human cancers and inflammatory diseases make it a critical molecular therapeutic target. In the present study we have mobilized a proximal twenty one bp nucleotide (21RY) which is in the promoter region (-55 to-75) of hmgb1 gene and targeted it with triplex forming oligonucleotide (TFO) in combination with two widely used chemotherapeutic drugs actinomycin (ACT) and adriamycin (ADM)...
February 13, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28267332/complexation-between-dna-and-hydrophilic-cationic-diblock-copolymers
#16
Seyoung Jung, Timothy P Lodge, Theresa M Reineke
We examine connections among polycation composition, DNA-polycation binding thermodynamics, binding strength, and resulting complex properties, for circular and linear DNA and hydrophilic diblock copolymers possessing cationic blocks. Two poly(2-deoxy-2-methacrylamido glucopyranose)-block-poly(N-(2-aminoethyl) methacrylamide) (PMAG-b-PAEMA), with block degrees of polymerization of PMAG56-b-PAEMA30 and PMAG52-b-PAEMA63, are employed. DNA binding behavior of these diblocks is also compared with that of a PAEMA homopolymer, in order to evaluate the role of the hydrophilic, charge-neutral PMAG block...
March 7, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28265459/indirect-tolerability-comparison-of-deutetrabenazine-and-tetrabenazine-for-huntington-disease
#17
Daniel O Claassen, Benjamin Carroll, Lisa M De Boer, Eric Wu, Rajeev Ayyagari, Sanjay Gandhi, David Stamler
BACKGROUND: Vesicular monoamine transporter 2 (VMAT2) inhibitors can improve hyperkinetic movements, and are effective treatment options for chorea of Huntington disease (HD). Tetrabenazine was assessed for treating chorea in the TETRA-HD trial, and while efficacious, there are tolerability concerns possibly due to its pharmacokinetic properties. Deutetrabenazine is a novel VMAT2 inhibitor that contains deuterium, which extends active metabolite half-lives and minimizes drug concentration fluctuations...
2017: Journal of Clinical Movement Disorders
https://www.readbyqxmd.com/read/28263598/interactions-between-oppositely-charged-polyelectrolytes-by-isothermal-titration-calorimetry-effect-of-ionic-strength-and-charge-density
#18
Feriel Meriem Lounis, Joseph Chamieh, Laurent Leclercq, Philippe Gonzalez, Amine Geneste, Benedicte Prelot, Hervé Cottet
In this study, binding of linear poly(L-lysine) to a series of acrylamide and 2-acrylamido-2-methyl-1-propanesulfonate copolymers was examined by isothermal titration calorimetry (ITC). Binding constant and stoichiometry were systematically determined at different ionic strengths and for different polyanion charge densities varying between 15% and 100%. The range of investigated ionic strengths was carefully adjusted according to the polyanion charge density to get measurable binding constants (i.e. formation binding constant typically comprised between 104 and 106 M-1) by isothermal titration calorimetry (ITC)...
March 6, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28261293/clinical-and-histopathological-characteristics-of-patients-with-incidental-and-nonincidental-thyroid-cancer
#19
Krzysztof Kaliszewski, Dorota Diakowska, Marta Strutyńska-Karpińska, Beata Wojtczak, Paweł Domosławski, Waldemar Balcerzak
INTRODUCTION: Thyroid nodules (TNs) are a common pathology. Their prevalence increases with age. Some of them are suspected of malignancy and qualified for surgery. Sometimes their malignant nature is detected incidentally after a surgical procedure. The aim of the study is to analyze clinical and histopathological characteristics of patients with incidental and nonincidental thyroid carcinoma (ITC vs. NITC). MATERIAL AND METHODS: The case records of 3,241 patients with solitary and multiple TNs who were treated consecutively between 2008 and 2014 were analyzed retrospectively...
March 1, 2017: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/28256648/investigation-of-the-interactions-between-methylene-blue-and-intramolecular-g-quadruplexes-an-explicit-distinction-in-electrochemical-behavior
#20
Ting Cao, Fang-Ting Zhang, Liang-Yuan Cai, Ying-Lin Zhou, Niklaas J Buurma, Xin-Xiang Zhang
G-quadruplex sequences exist in eukaryotic organisms and prokaryotes, and the investigation of the interactions between G-quadruplexes and small molecule ligands is important for gene therapy, biosensor fabrication, fluorescence imaging and so on. Here, we investigated the behaviour of methylene blue (MB), an electroactive molecule, in the presence of different intramolecular G-quadruplexes by an electrochemical method using a miniaturized electrochemical device based on its intrinsic electrochemical properties...
March 13, 2017: Analyst
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