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Krzysztof Brzezinski, Justyna Czyrko, Joanna Sliwiak, Edyta Nalewajko-Sieliwoniuk, Mariusz Jaskolski, Boguslaw Nocek, Zbigniew Dauter
Thermotoga maritima is a hyperthermophilic bacterium but its genome encodes a number of archaeal proteins including S-adenosyl-L-homocysteine hydrolase (SAHase), which regulates cellular methylation reactions. The question of proper folding and activity of proteins of extremophilic origin is an intriguing problem. When expressed in E.coli and purified (as a homotetramer) at room temperature, the hyperthermophilic SAHase from T.maritima was inactive. ITC study indicated that the protein undergoes heat-induced conformational changes, and enzymatic activity assays demonstrated that these changes are required to attain enzymatic activity...
June 16, 2017: International Journal of Biological Macromolecules
Zejun Li, Tara Elton-Marshall, Geoffrey T Fong, Anne Chiew Kin Quah, Guoze Feng, Yuan Jiang, Sara C Hitchman
No abstract text is available yet for this article.
June 19, 2017: BMC Public Health
Fa-Jie Li, Yuan Liu, Yuan Yuan, Bin Yang, Zhen-Ming Liu, Lu-Qi Huang
(-)-Epicatechin gallate ((-)-ECG), 1,2,3,4,6-O-pentagalloylglucose (PGG), rhodionin, herbacetin and rhodiosin isolated from the root of Rhodiola crenulata exhibited potent, dose-dependent inhibitory effects on acetylcholinesterase (AChE) with IC50 ranged from 57.50±5.83 to 2.43±0.34μg/mL. With the aim of explaining the differences in activity of these active ingredients and clarifying how they inhibit AChE, the AChE-inhibitor interactions were further explored using molecular docking and isothermal titration calorimetry (ITC) methods in the present study...
June 15, 2017: International Journal of Biological Macromolecules
Fabio Martinelli, Antonino Ditto, Mauro Signorelli, Giorgio Bogani, Valentina Chiappa, Domenica Lorusso, Cono Scaffa, Dario Recalcati, Stefania Perotto, Edward Haeusler, Francesco Raspagliesi
OBJECTIVES: To analyze detection-rate(DR) and diagnostic-accuracy (A) of sentinel-nodes(SLNs) mapping following hysteroscopic-injection of tracer. To compare DR and A between tracers: ICG and Tc99m. METHODS: Evaluation of endometrial-cancer patients who underwent SLNs mapping after hysteroscopic-peritumoral-injection of tracer±lymphadenectomy. Analysis of DR (overall-bilateral-aortic) and A in the entire cohort and comparison between tracers. RESULTS: 202 procedures were performed from January/2005 to February/2017...
June 15, 2017: Gynecologic Oncology
Salvador F Ausar, Kavisha Jayasundara, Lamees Akawi, Cristopher Roque, Anthony Sheung, Jian Hu, Marina Kirkitadze, Nausheen Rahman
The Pneumococcal Histidine Triad Protein D (PhtD) is believed to play a central role in pneumococcal metal ion homeostasis and has been proposed as a promising vaccine candidate against pneumococcal disease. To investigate for potential stabilizers, a panel of physiologically relevant metals was screened using the thermal shift assay and it was found that only Zn(2+) and Mn(2+) were able to increase PhtD melting temperature. Differential Scanning Calorimetry (DSC) analysis revealed a sequential unfolding of PhtD and the presence of at least 3 independent folding domains that can be stabilized by Zn(2+) and Mn(2+)...
June 14, 2017: Journal of Pharmaceutical Sciences
Christopher R Bodle, Duncan I Mackie, Michael P Hayes, Josephine H Schamp, Michael R Miller, Michael D Henry, Jonathan A Doorn, Jon C D Houtman, Michael A James, David L Roman
Regulator of G Protein Signaling (RGS) 17 is an overexpressed promoter of cancer survival in lung and prostate tumors, the knockdown of which results in decreased tumor cell proliferation in vitro. Identification of drug-like molecules inhibiting this protein could ameliorate the RGS17's pro-tumorigenic effect. Using high-throughput screening, a chemical library containing natural products was interrogated for inhibition of the RGS17-Gαo interaction. Initial hits were verified in control and counter screens...
June 16, 2017: Journal of Natural Products
Lijun Sun, Michael J Gidley, Fredrick J Warren
SCOPE: This study aims to use a combination of biochemical and biophysical methods to derive greater mechanistic understanding of the interactions between tea polyphenols and porcine pancreatic α-amylase (PPA). METHODS AND RESULTS: The interaction mechanism was studied through fluorescence quenching (FQ), differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC) and compared with inhibition kinetics. The results showed that a higher quenching effect of polyphenols corresponded to a stronger inhibitory activity against PPA...
June 15, 2017: Molecular Nutrition & Food Research
Rose Davidson, Sarah Gardner, Orla Jupp, Angela Bullough, Sue Butters, Laura Watts, Simon Donell, Maria Traka, Shikha Saha, Richard Mithen, Mandy Peffers, Peter Clegg, Yongping Bao, Aedin Cassidy, Ian Clark
Osteoarthritis is a major cause of disability and there is no current pharmaceutical treatment which can prevent the disease or slow its progression. Dietary advice or supplementation is clearly an attractive option since it has low toxicity and ease of implementation on a population level. We have previously demonstrated that sulforaphane, a dietary isothiocyanate derived from its glucosinolate precursor which is found in broccoli, can prevent cartilage destruction in cells, in in vitro and in vivo models of osteoarthritis...
June 13, 2017: Scientific Reports
Aki Hashimoto, Daisuke Hagiwara, Akira Watanabe, Maki Yahiro, Alimu Yikelamu, Takashi Yaguchi, Katsuhiko Kamei
Aspergillus niger and its related species, known as Aspergillus section Nigri, are ubiquitously distributed across the globe and are often isolated from clinical specimens. In Japan, Aspergillus section Nigri is the second-most often isolated from clinical specimens following A. fumigatus. We determined the species of Aspergillus section Nigri isolated in Japan by DNA sequencing of partial β-tubulin genes and investigated drug susceptibility by CLSI M38-A2 method. The collection contained 20 A. niger, 59 A...
June 12, 2017: Antimicrobial Agents and Chemotherapy
Antoine Hoang, Eugénie Laigre, David Goyard, Eric Defrancq, Françoise Vinet, Pascal Dumy, Olivier Renaudet
Carbohydrate microarrays represent powerful tools to study and detect carbohydrate-binding proteins, pathogens or cells. In this paper, we report two original oxime-based methods to prepare surfaces displaying well-defined structures and valency in a given microspot with improved recognition potency with lectins. In a first "direct" approach, fully synthetic aminooxylated glycoclusters have been coated onto aldehyde-activated SiO2 (silicium substrate doped with 50 nm thermal oxide layer). To improve the preparation of the microarray in terms of rapidity and simplicity and to provide addressable surfaces on which sugars can be linked chemoselectively as clusters at defined plots, a second "indirect" strategy has been developed using successive oxime ligation steps...
June 21, 2017: Organic & Biomolecular Chemistry
Bruno Aquino, Rafael M Couñago, Natalia Verza, Lucas M Ferreira, Katlin B Massirer, Opher Gileadi, Paulo Arruda
Kinases are primary regulators of plant metabolism and excellent targets for plant breeding. However, most kinases, including the abundant receptor-like kinases (RLK), have no assigned role. SIRK1 is a leucine-rich repeat receptor-like kinase (LRR-RLK), the largest family of RLK. In Arabidopsis thaliana, SIRK1 (AtSIRK1) is phosphorylated after sucrose is resupplied to sucrose-starved seedlings and it modulates the sugar response by phosphorylating several substrates. In maize, the ZmSIRK1 expression is altered in response to drought stress...
2017: Frontiers in Plant Science
Li-Yun Yang, Si-Yu Hua, Zhi-Qiang Zhou, Guan-Chao Wang, Feng-Lei Jiang, Yi Liu
Fullerenols, known as polyhydroxylated derivatives of fullerene, have attracted great attention due to their distinctive material properties and potential applications in biology and medicine. As a step toward the elucidation of basic behavior in biological systems, a variety of spectroscopic measurements as well as isothermal titration calorimetry (ITC) were applied to study the interaction between fullerenol (C60(OH)44) and serum proteins (bovine serum albumin (BSA) and γ-globulins). The results of fluorescence spectra indicated that the intrinsic fluorescence of proteins could be effectively quenched by the dynamic mechanism...
May 31, 2017: Colloids and Surfaces. B, Biointerfaces
Iñigo Espinosa, Neus Serrat, Gian Franco Zannoni, Ramón Rovira, Emanuela D'Angelo, Jaime Prat
In endometrioid endometrial carcinomas (EEC), MELF (microcystic, elongated and fragmented) myoinvasion is associated with easily overlooked lymph node metastases; however, the role of immunohistochemistry in their detection and their clinical significance have not been addressed. We identified MELF in 43 of 101 (43%) myoinvasive EECs. Nodes were removed in 49 (49%), 25 with MELF and 24 without MELF. Metastases were initially reported in 3 of the former (12%) and 2 of the latter (8%). All negative nodes were reviewed and cytokeratin immunohistochemistry was performed...
June 7, 2017: Human Pathology
Hongfeng Chen, Luuk K Koopal, Juan Xiong, Marcelo Avena, Wenfeng Tan
To explore the adsorption mechanisms of a soil humic acid (HA) on purified kaolinite and montmorillonite, a combination of adsorption measurements, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and isothermal titration calorimetry (ITC) was employed at pH 4.0, 6.0 and 8.0. The adsorption affinities and plateaus of HA on the two clays increased with decreasing pH, indicating the importance of electrostatic interaction. The effects were more significant for kaolinite than for montmorillonite...
May 24, 2017: Journal of Colloid and Interface Science
Shenghao Xu, Teng Gao, Xiuying Feng, Xiaojian Fan, Gufan Liu, Yaning Mao, Xijuan Yu, Jiehua Lin, Xiliang Luo
Here, a multidimensional sensor array capable of analyzing various proteins and discriminating between serums from different stages of breast cancer patients were developed based on six kinds of near infrared fluorescent dual ligand functionalized Au NCs (functionalized with different amino acids) as sensing receptors. These six kinds of different amino acids functionalized Au NCs were synthesized for the first time within 2h due to the direct donation of delocalized electrons of electron-rich atoms or groups of the ligands to the Au core...
June 6, 2017: Biosensors & Bioelectronics
Justin M Di Trani, Nicolas Moitessier, Anthony K Mittermaier
Isothermal titration calorimetry (ITC) is a powerful tool for acquiring both thermodynamic and kinetic data for biological interactions including molecular recognition and enzymatic catalysis. ITC-based kinetics measurements typically focus on reactions taking place over long time scales (tens of minutes or hours) in order to avoid complications due to the finite length of time needed detect heat flow in the calorimeter cell. While progress has been made toward analyzing more rapid reaction kinetics by ITC, the capabilities and limitations of this approach have not been thoroughly tested to date...
June 21, 2017: Analytical Chemistry
M R Rivero, M M Salas, R Valente, M J Nores, C De Angelo, J Arrabal, S Costa, O D Salomón
To investigate knowledge of school-aged children and their perception on intestinal parasites, and to assess knowledge reconstruction on prevention practices after specific training in the subject. We performed an activity package that included the analysis of children's drawings of intestinal parasites, and information and communication technologies (ITCs) to transfer knowledge about these pathogens and prevention measures. Retrieval learning activities were performed to fixation of general and specific prevention and control measures...
June 7, 2017: Zoonoses and Public Health
Yongbo Qi, Jun Zhu, Qingling Fu, Hongqing Hu, Qiaoyun Huang
The sorption of Cu on humic acid (HA) from the decomposition of rice straw in the absence (Ck-HA) and presence of montmorillonite (M-HA), kaolinite (K-HA), gibbsite (Gi-HA) and goethite (Go-HA) was investigated at pH 5.0 by using batch studies combined with isothermal titration calorimetry (ITC) and atomic force microscopy (AFM). Characterization by elemental analysis and potentiometric titration showed the composition difference among these five HA. The sorption capacity and rate increased in the order: M-HA < K-HA < Gi-HA < Ck-HA < Go-HA...
June 3, 2017: Journal of Environmental Management
Shweta Ganapati, Stephanie D Grabitz, Steven Murkli, Flora Scheffenbichler, Maira I Rudolph, Peter Y Zavalij, Matthias Eikermann, Lyle Isaacs
We measured the affinity of five molecular container compounds (Calabadion 1 and 2, CB[7], sulfocalix[4]arene and HP-β-CD) toward seven drugs of abuse in homogenous aqueous solution at physiological pH by various (1H NMR, UV/Vis, ITC) binding assays and find that they span from less than 102 to over 108 M-1. X-ray crystal structures of CB[7]*methamphetamine and 1*methamphetamine are reported. We find that 2, but not CB[7], is able to ameliorate the hyperlocomotive activity of rats treated with methamphetamine...
June 6, 2017: Chembiochem: a European Journal of Chemical Biology
Marie Plante, Jonathan Stanleigh, Marie-Claude Renaud, Alexandra Sebastianelli, Katherine Grondin, Jean Grégoire
OBJECTIVE: To evaluate the outcome and the role of adjuvant treatment in the management of patients with endometrial cancer and isolated tumor cells (ITCs) identified by SLN mapping. METHODS: This single center study identified all patients undergoing hysterectomy, salpingo-oophorectomy, lymphadenectomy and SLN mapping for endometrial cancer between November 2010 and December 2015. Data was prospectively collected. Progression-free survival was analyzed according to the Kaplan-Meier method and compared using the log-rank test...
May 31, 2017: Gynecologic Oncology
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