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https://www.readbyqxmd.com/read/28942993/performance-of-sentinel-lymph-node-sln-mapping-in-high-risk-endometrial-cancer
#1
Omar Touhami, Jean Grégoire, Marie-Claude Renaud, Alexandra Sebastianelli, Marie Plante
OBJECTIVE: While the accuracy of the SLN procedure has been validated in patients with low risk EC, its relevance for high-risk EC remains debated. The aim of this study was to evaluate the accuracy of SLN mapping in patients with high-risk EC. STUDY METHOD: We reviewed all patients with high risk EC (grade 3 endometrioid, serous, carcinosarcoma, clear cell and undifferentiated) who underwent primary surgery with SLN mapping followed by pelvic +/- paraaortic lymphadenectomy, between November 2010 and November 2016...
September 21, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28938865/binding-of-ibuprofen-to-human-hemoglobin-elucidation-of-their-molecular-recognition-by-spectroscopy-calorimetry-and-molecular-modeling-techniques
#2
Paromita Seal, Jyotirmoy Sikdar, Amartya Roy, Rajen Haldar
Ibuprofen, used for the treatment of acute and chronic pain, osteoarthritis, rheumatoid arthritis, and related conditions has ample affinity to globular proteins. Here we have explored this fundamental study pertaining to the interaction of ibuprofen with human hemoglobin (HHb), using multispectroscopic, calorimetric, and molecular modeling techniques to gain insights into molecular aspects of binding mechanism. Ibuprofen-induced graded decrease in absorption spectra indicates protein disruption along with sedimentation of HHb particle...
September 22, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28932547/does-lung-cancer-surgery-cause-circulating-tumor-cells-a-multicenter-prospective-study
#3
Noriyuki Matsutani, Noriyoshi Sawabata, Masafumi Yamaguchi, Tetsukan Woo, Yujin Kudo, Akikazu Kawase, Satoshi Shiono, Hisae Iinuma, Shigeki Morita, Masafumi Kawamura
BACKGROUND: Cancer relapse is caused by residual isolated tumor cells (ITCs) remaining in the body after surgery. It is speculated that surgical manipulation may cause circulating tumor cells (CTCs) which are the origin of ITCs in the body. The occurrence of CTCs in surgical patients with non-small cell lung cancer (NSCLC) has been shown in retrospective observation, but not prospectively, thus we conducted a multicenter prospective study regarding the occurrence of CTCs by surgical manipulation...
August 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28926243/are-we-there-yet-applying-thermodynamic-and-kinetic-profiling-on-embryonic-ectoderm-development-eed-hit-to-lead-program
#4
Ying Wang, Rohinton P Edalji, Sanjay C Panchal, Chaohong C Sun, Stevan W Djuric, Anil Vasudevan
It is advocated that kinetic and thermodynamic profiling of bioactive compounds should be incorporated and utilized as complementary tools for hit and lead optimizations in drug discovery. To assess their applications in the EED hit-to-lead optimization process, large amount of thermodynamic and kinetic data were collected and analyzed via isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR), respectively. Slower dissociation rates (koff) of the lead compounds were observed as the program progressed...
September 19, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28925982/osmolyte-like-stabilizing-effects-of-low-gdnhcl-concentrations-on-d-glucose-d-galactose-binding-protein
#5
Alexander V Fonin, Alexandra D Golikova, Irina A Zvereva, Sabato D'Auria, Maria Staiano, Vladimir N Uversky, Irina M Kuznetsova, Konstantin K Turoverov
The ability of d-glucose/d-galactose-binding protein (GGBP) to reversibly interact with its ligands, glucose and galactose, makes this protein an attractive candidate for sensing elements of glucose biosensors. This potential is largely responsible for attracting researchers to study the conformational properties of this protein. Previously, we showed that an increase in the fluorescence intensity of the fluorescent dye 6-bromoacetyl-2-dimetylaminonaphtalene (BADAN) is linked to the holo-form of the GGBP/H152C mutant in solutions containing sub-denaturing concentrations of guanidine hydrochloride (GdnHCl)...
September 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28922641/structural-luminescence-thermodynamic-and-theoretical-studies-on-mononuclear-complexes-of-eu-iii-with-pyridine-monocarboxylate-n-oxides-in-aqueous-solution
#6
Rama Mohana Rao Dumpala, Neetika Rawat, Anil Boda, Sk Musharaf Ali, B S Tomar
The mononuclear complexes formed by Eu(III) with three isomeric pyridine monocarboxylate-N-oxides namely picolinic acid-N-oxide (PANO), nicotinic acid-N-oxide (NANO) and isonicotinic acid-N-oxide (IANO) in aqueous solutions were studied by potentiometry, luminescence spectroscopy and isothermal titration calorimetry (ITC) to determine the speciation, coordination, luminescence properties and thermodynamic parameters of the complexes formed during the course of the reaction. More stable six membered chelate complexes with stoichiometry (MLi, i=1-4) are formed by Eu(III) with PANO while non chelating ML and ML2 complexes are formed by NANO and IANO...
September 9, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28919846/a-methylidene-group-in-the-phosphonic-acid-analogue-of-phenylalanine-reverses-the-enantiopreference-of-binding-to-phenylalanine-ammonia-lyases
#7
Zsófia Bata, Renzhe Qian, Alexander Roller, Jeannie Horak, László Csaba Bencze, Csaba Paizs, Friedrich Hammerschmidt, Beáta G Vértessy, László Poppe
Aromatic amino acid ammonia-lyases and aromatic amino acid 2,3-aminomutases contain the post-translationally formed prosthetic 3,5-dihydro-4-methylidene-5H-imidazol-5-one (MIO) group. MIO enzymes catalyze the stereoselective synthesis of α- or β-amino acid enantiomers, making these chemical processes environmentally friendly and affordable. Characterization of novel inhibitors enables structural understanding of enzyme mechanism and recognizes promising herbicide candidates as well. The present study found that both enantiomers of the aminophosphonic acid analogue of the natural substrate phenylalanine and a novel derivative bearing a methylidene at the β-position inhibited phenylalanine ammonia-lyases (PAL), representing MIO enzymes...
June 19, 2017: Advanced Synthesis & Catalysis
https://www.readbyqxmd.com/read/28919611/incidental-thyroid-carcinoma-in-patients-treated-surgically-for-presumably-benign-thyroid-disease
#8
M M Rahman, U S Abdullah, A I Joarder, C Das, B C Debnath, R Sah
Incidental Thyroid Carcinoma (ITC) is quite high as been reported in the world's Journal. This study reviews the frequency of the ITC in patients treated surgically for otherwise benign Thyroid disease in one of the Endocrine surgery unit of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh. This study was developed by the investigating the outcome of one hundred and twenty seven patient who underwent partial or total thyroidectomy for benign thyroid pathology in a single Endocrine Surgery Unit of BSMMU from January 2011 to July 2015...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28914483/the-thermodynamic-basis-of-the-fuzzy-interaction-of-an-intrinsically-disordered-protein
#9
San Hadži, Andrej Mernik, Črtomir Podlipnik, Remy Loris, Jurij Lah
Many intrinsically disordered proteins (IDP) that fold upon binding retain conformational heterogeneity in IDP-target complexes. The thermodynamics of such fuzzy interactions is poorly understood. Here we introduce a thermodynamic framework, based on analysis of ITC and CD spectroscopy data, that provides experimental description of IDP association in terms of folding and binding contributions which can be predicted using sequence folding propensities and molecular modeling. We show how IDP can modulate the entropy and enthalpy by adapting their bound-state structural ensemble to achieve optimal binding...
September 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28910978/dna-binding-properties-of-foxp3-transcription-factor
#10
Jun Li, Longying Jiang, Xujun Liang, Lingzhi Qu, Daichao Wu, Xiaojuan Chen, Ming Guo, Zhuchu Chen, Lin Chen, Yongheng Chen
FOXP3, a lineage-specific forkhead (FKH) transcription factor, plays essential roles in the development and function of regulatory T cells. However, the DNA-binding properties of FOXP3 are not well understood. In this study, FOXP3 fragments containing different domains were purified, and their DNA-binding properties were investigated using electrophoretic mobility shift assay and isothermal titration calorimetry (ITC). Both the FKH and leucine-zipper domains were required for optimal DNA binding for FOXP3. FOXP3 protein not only binds with DNA sequences containing one FKH consensus sequence, but also binds with DNA sequences with two direct repeats of consensus sequences separated by three-nucleotides (DRE3)...
September 1, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28910094/hemicellulosic-polysaccharides-mimics-synthesis-of-tailored-bottlebrush-like-xyloglucan-oligosaccharide-glycopolymers-as-binders-of-nanocrystalline-cellulose
#11
Jing Chen, Christophe Travelet, Redouane Borsali, Sami Halila
We report in this contribution that while low molecular weight hemicellulosic building blocks are known not to interact with cellulosic materials, their multivalent presentation on a polymeric scaffold significantly enhanced the binding interactions that are remarkably in the same range as those usually observed for lectin-carbohydrate interactions. We developed a poly(propargyl methacrylate) scaffold on which we conjugated, by "post-click" reaction, a variety of azide reducing-end functionalized xyloglucan oligosaccharides with controlled enzymatic-mediated rate of degalactosylation...
September 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28904931/isolation-of-candida-species-from-gastroesophageal-lesions-among-pediatrics-in-isfahan-iran-identification-and-antifungal-susceptibility-testing-of-clinical-isolates-by-e-test
#12
Fatemeh Salehi, Mehran Esmaeili, Rasoul Mohammadi
BACKGROUND: Candida species can become opportunistic pathogens causing local or systemic invasive infections. Gastroesophageal candidiasis may depend on the Candida colonization and local damage of the mucosal barrier. Risk factors are gastric acid suppression, diabetes mellitus, chronic debilitating states such as carcinomas, and the use of systemic antibiotics and corticosteroids. The aim of this study is collection and molecular identification of Candida species from gastroesophageal lesions among pediatrics in Isfahan, and determination of minimum inhibitory concentration (MIC) ranges for clinical isolates...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28902442/spectroscopic-investigations-on-the-conformational-changes-of-lysozyme-effected-by-different-sizes-of-n-acetyl-l-cysteine-capped-cdte-quantum-dots
#13
Qianqian Wu, Jingqiang Wan, Zhuo He, Rutao Liu
The effect of N-acetyl-l-cysteine-capped CdTe quantum dots (NAC-CdTe QDs) with different sizes on lysozyme was investigated by isothermal titration calorimetry (ITC), enzyme activity assays, and multi-spectroscopic methods. ITC results proved that NAC-CdTe QDs can spontaneously bind with lysozyme and hydrophobic force plays a major role in stabilizing QDs-lysozyme complex. Multi-spectroscopic measurements revealed that NAC-CdTe QDs caused strong quenching of the lysozyme's fluorescence in a size-dependent quenching manner...
September 13, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28898988/comparison-of-oncotype-dx-with-modified-magee-equation-recurrence-scores-in-low-grade-invasive-carcinoma-of-breast
#14
Yanjun Hou, Debra L Zynger, Xiaoxian Li, Zaibo Li
Objectives: Several specific histologic types of invasive carcinoma of breast, including invasive tubular carcinoma (ITC), invasive mucinous carcinoma (IMC), and classical invasive lobular carcinoma (CILC), are considered low-grade carcinomas with favorable outcomes. We aimed to investigate the utility of Oncotype DX test in these tumors by comparing its recurrence score (RS) with the modified Magee equation RS. Methods: Oncotype DX RSs were collected and modified Magee equation RSs were calculated in 163 low-grade invasive breast carcinomas, including 105 CILCs, 41 ITCs, and 17 IMCs...
August 1, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28894220/binding-modes-of-environmental-endocrine-disruptors-to-human-serum-albumin-insights-from-std-nmr-itc-spectroscopic-and-molecular-docking-studies
#15
Hongqin Yang, Yanmei Huang, Jiuyang Liu, Peixiao Tang, Qiaomei Sun, Xinnuo Xiong, Bin Tang, Jiawei He, Hui Li
Given that bisphenols have an endocrine-disrupting effect on human bodies, thoroughly exposing their potential effects at the molecular level is important. Saturation transfer difference (STD) NMR-based binding studies were performed to investigate the binding potential of two bisphenol representatives, namely, bisphenol B (BPB) and bisphenol E (BPE), toward human serum albumin (HSA). The relative STD (%) suggested that BPB and BPE show similar binding modes and orientations, in which the phenolic rings were spatially close to HSA binding site...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28891642/supramolecular-host-guest-system-as-ratiometric-fe3-ion-sensor-based-on-water-soluble-pillar-5-arene
#16
Qianfang Yao, Baozhong Lv, Chendong Ji, Yang Cai, Meizhen Yin
Developing a specific, ratiometric and reversible detection method for metal ions is significant to against the threat of metal-caused environment pollution and organisms poisoning. Here a supramolecular host-guest system (WP5⊃G) based on water-soluble pillar[5]arene (WP5) and water-soluble quaternized perylene diimide derivative (G) was constructed. Morphological transformation was achieved during the process of adding WP5 into G aqueous solution and a fluorescence "turn-off" phenomenon was observed which caused by supramolecular photoinduced electron transfer (PET)...
September 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28889310/isothermal-titration-calorimetry-to-determine-apparent-dissociation-constants-k-d-and-stoichiometry-of-interaction-n-of-c-di-gmp-binding-proteins
#17
Bruno Y Matsuyama, Petya V Krasteva, Marcos V A S Navarro
Isothermal titration calorimetry (ITC) is a commonly used biophysical technique that enables the quantitative characterization of intermolecular interactions in solution. Based on enthalpy changes (ΔH) upon titration of the binding partner (e.g., a small-molecule ligand such as c-di-GMP) to the molecule of interest (e.g., a receptor protein), the resulting binding isotherms provide information on the equilibrium association/dissociation constants (K a, K d) and stoichiometry of binding (n), as well as on changes in the Gibbs free energy (ΔG) and entropy (ΔS) along the interaction...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28888786/microwave-assisted-synthesis-of-ruthenium-ii-complexes-with-alkynes-as-potential-inhibitor-by-selectively-recognizing-c-myc-g-quadruplex-dna
#18
Shuangyan Zhang, Qiong Wu, Hao Zhang, Qi Wang, Xicheng Wang, Wenjie Mei, Xiaohui Wu, Wenjie Zheng
Herein, two polypyridyl ruthenium(II) complexes with alkynes, [Ru(bpy)2L](ClO4)2 (L=p-TEPIP (1) and p-BEPIP (2); bpy=2,2'-bipyridine; p-TEPIP=2-(4-trimethylsilylpropargyl)-1H-imidazo[4,5f][1,10]phenanthroline; p-BEPIP=2-(4-phenyacetylenephenyl)-1H-imidazo[4,5f][1,10]phenanthroline) have been successfully achieved in yields of 32%-89% by a Sonogashira coupling reaction under microwave irradiation. We studied these complexes as potential stabilizers of c-myc G-quadruplex DNA. Observations revealed that both complexes could selectively bind to and stabilize c-myc G-quadruplex DNA with a constant of approximately 1...
August 30, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28886512/physicochemical-study-of-biomolecular-interactions-between-lysosomotropic-surfactants-and-bovine-serum-albumin
#19
Tomasz Janek, Żaneta Czyżnikowska, Jacek Łuczyński, Eduardo J Gudiña, Lígia R Rodrigues, Joanna Gałęzowska
The interactions between two cationic lysosomotropic surfactants (2-dodecanoyloxyethyl)trimethylammonium bromide (DMM-11) and (2-dodecanoyloxypropyl)trimethylammonium bromide (DMPM-11) with bovine serum albumin (BSA) in Hepes buffer (pH=7.4) were systematically studied by surface tension, fluorescence and circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC). Furthermore, the size of the micellar aggregates and the polydispersity indexes of both cationic surfactants were studied by dynamic light scattering technique (DLS)...
August 30, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28885827/attachment-site-cysteine-thiol-pka-is-a-key-driver-for-site-dependent-stability-of-thiomab-antibody-drug-conjugates
#20
Breanna S Vollmar, Binqing Wei, Rachana Ohri, Jianhui Zhou, Jintang He, Shang-Fan Yu, Douglas Leipold, Ely Cosino, Sharon Yee, Aimee Fourie-O'Donohue, Guangmin Li, Gail L Phillips, Katherine R Kozak, Amrita Kamath, Keyang Xu, Genee Lee, Greg A Lazar, Hans K Erickson
The incorporation of cysteines into antibodies by mutagenesis allows for the direct conjugation of small molecules to specific sites on the antibody via disulfide bonds. The stability of the disulfide bond linkage between the small molecule and the antibody is highly dependent on the location of the engineered cysteine in either the heavy chain (HC) or the light chain (LC) of the antibody. Here, we explore the basis for this site-dependent stability. We evaluated the in vivo efficacy and pharmacokinetics of five different cysteine mutants of trastuzumab conjugated to a pyrrolobenzodiazepine (PBD) via disulfide bonds...
September 22, 2017: Bioconjugate Chemistry
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