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https://www.readbyqxmd.com/read/28645190/pcr-rflp-on-the-rdna-cannot-unequivocally-assign-ascaris-to-species-level-or-identify-hybrids
#1
Peter Nejsum, Martha Betson, Russell Stothard
No abstract text is available yet for this article.
June 22, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28633651/epidemiology-of-intestinal-helminthiasis-among-school-children-with-emphasis-on-schistosoma-mansoni-infection-in-wolaita-zone-southern-ethiopia
#2
Bereket Alemayehu, Zewdneh Tomass, Fiseha Wadilo, Dawit Leja, Song Liang, Berhanu Erko
BACKGROUND: Intestinal helminth infections are major parasitic diseases causing public health problems in Ethiopia. Although the epidemiology of these infections are well documented in Ethiopia, new transmission foci for schistosomiasis are being reported in different parts of the country. The objective of this study was to assess the prevalence of Schistosoma mansoni and other intestinal helminth infections among school children and determine the endemicity of schistosomiasis in Wolaita Zone, southern Ethiopia...
June 20, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28632749/using-a-health-rating-system-to-evaluate-the-usefulness-of-caenorhabditis-elegans-as-a-model-for-anthelmintic-study
#3
Kathryn J Weaver, Cassandra J May, Brian L Ellis
Soil-transmitted helminths (STHs) are intestinal parasitic nematodes that infect humans, and are transmitted through contaminated soil. These nematodes include the large roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), and hookworm (Ancylostoma ceylanicum, Ancylostoma duodenale, and Necator americanus). Nearly 1.5 billion people (~24% of the population) worldwide are infected with at least one species of these parasites, burdening the poor, in particular, children and pregnant women. To combat these diseases, the WHO only recognizes four anthelmintic drugs, including the preferred drug, albendazole, for mass drug administration (MDA)...
2017: PloS One
https://www.readbyqxmd.com/read/28615864/prevalence-of-gastro-intestinal-parasites-in-pigs-in-punjab-india
#4
Mandeep Kaur, Balbir Bagicha Singh, Rajnish Sharma, Jatinder Paul Singh Gill
Gastrointestinal parasites are a common problem in pigs in India. The important risk factors include coprophagic behaviour of pigs and their free access to garbage. To investigate the gastrohelmenthic spectrum in pigs of Punjab, we examined 265 faecal samples from farm (n = 47) and scavenging pigs (n = 218) using faecal floatation method. Ascaris suum, unsporulated oocysts, Trichuris spp. and Strongyloides were recorded in 27.5, 15.4, 1.8 and 4.5 % of the pig faecal samples, respectively. Overall prevalence was significantly higher in pigs >1 year (56...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28615087/association-between-intestinal-helminth-infections-and-mid-upper-arm-circumference-among-children-in-sri-lanka-a-cross-sectional-study
#5
L S Galgamuwa, D Iddawela, S D Dharmaratne
Intestinal helminth infections constitute a major health problem in low socio-economic communities worldwide. A cross-sectional study was conducted in children aged between 12 and 60 months. A structured questionnaire was used to collect socio-demographic data and stool samples were subjected to direct smear, stained with Lugol's iodine, and formaldehyde-ether concentration techniques to determine the prevalence of intestinal helminth infections. The circumference of each child's left mid upper arm (MUAC) was measured using standard procedures...
June 15, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28606164/the-importance-of-patient-compliance-in-repeated-rounds-of-mass-drug-administration-mda-for-the-elimination-of-intestinal-helminth-transmission
#6
Sam H Farrell, James E Truscott, Roy M Anderson
BACKGROUND: Systematic non-compliance to chemotherapeutic treatment among a portion of the eligible population is thought to be a major obstacle to the elimination of helminth infections by mass drug administration (MDA). MDA for helminths is repeated at defined intervals such as yearly or every 2 years, as a consequence of the inability of the human host to develop fully protective immunity to reinfection. As such, how an individual complies to these repeated rounds of MDA can have a significant impact on parasite transmission...
June 12, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28599671/is-there-a-difference-in-prevalence-of-helminths-between-households-using-ecological-sanitation-and-those-using-traditional-pit-latrines-a-latrine-based-cross-sectional-comparative-study-in-malawi
#7
Save Kumwenda, Chisomo Msefula, Wilfred Kadewa, Yohane Diness, Charles Kato, Tracy Morse, Bagrey Ngwira
BACKGROUND: Studies have shown that households using sludge from human excreta for agriculture are at an increased risk of soil transmitted helminths. However, while use of ecological sanitation (EcoSan) latrines is increasing in most African countries including Malawi, few studies have been done to check whether use of such sludge could potentially increase the prevalence of helminthic infections among household members as a results of exposure to faecal sludge/compared to use of traditional latrines...
June 9, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28592266/urgent-needs-in-fostering-neglected-tropical-diseases-ntds-laboratory-capacity-in-who-western-pacific-region-results-from-the-external-quality-assessment-on-ntds-diagnosis-in-2012-2015
#8
Yan Lu, Glenda Gonzales, Shao-Hong Chen, Hao Li, Yu-Chun Cai, Yan-Hong Chu, Lin Ai, Mu-Xin Chen, Hai-Ning Chen, Jia-Xu Chen
BACKGROUND: Neglected tropical diseases (NTDs) are a heterogeneous group of mainly chronic, debilitating and often stigmatizing diseases that largely affects low-income and politically marginalized populations, causing a large burden of public health, social and economies in the NTDs endemic countries. NTDs are caused by infections with a range of pathogen, including bacteria, parasites, protozoa and viruses. The accurate diagnosis of NTDs is important for reducing morbidity, preventing mortality and for monitoring of control programs...
June 8, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28590167/effects-of-in-vivo-and-in-vitro-treatment-of-ascaris-suum-eggs-with-anthelmintic-agents-on-embryonation-and-infectivity-for-mice
#9
Jianguo Zhao, Qian Han, Chenghong Liao, Jinhua Wang, Lili Wu, Qun Liu, David S Lindsay
Ascaris suum is an important intestinal nematode causing economic losses in the swine production industry. Anthelminthic treatment is used to control A. suum infections and is part of normal production practices. Treatment with anthelminthic agents results in expulsion of adult worms from the intestinal tract and ends further contamination of the environment with eggs. The present study was conducted to determine the effects of drug treatment on the embryonation of A. suum eggs collected from worms obtained from pigs treated with 4 different commercially available anthelmintics...
June 7, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28588624/distribution-of-parasites-detected-in-stool-samples-of-patients-in-le-dantec-university-hospital-of-dakar-senegal-from-2011-to-2015
#10
Khadim Diongue, Mouhamadou Ndiaye, Mame Cheikh Seck, Mamadou Alpha Diallo, Yaye Dié Ndiaye, Aïda Sadikh Badiane, Daouda Ndiaye
To identify the parasites responsible for intestinal parasitic infections diagnosed at Le Dantec University Hospital of Dakar, distribution of parasites detected in stool samples of patients was studied. From 2011 to 2015, 2578 patients were included in the study. A direct examination and Ritchie technique were performed as parasite search techniques. In total, 408 samples were positive showing 440 intestinal parasites; this corresponds to prevalence of 15.8%. Parasites were detected in monoparasitism (85.7%) and multiparasitism (14...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28582530/an-in-vivo-genetic-screen-for-genes-involved-in-spliced-leader-trans-splicing-indicates-a-crucial-role-for-continuous-de-novo-spliced-leader-rnp-assembly
#11
Lucas Philippe, George C Pandarakalam, Rotimi Fasimoye, Neale Harrison, Bernadette Connolly, Jonathan Pettitt, Berndt Müller
Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the precise details of SL trans-splicing have yet to be elucidated. In part, this is because the systematic identification of the molecules involved has not previously been possible due to the lack of a specific phenotype associated with defects in this process...
June 5, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28571565/c-reactive-protein-is-differentially-modulated-by-co-existing-infections-vitamin-deficiencies-and-maternal-factors-in-pregnant-and-lactating-indigenous-panamanian-women
#12
Doris González-Fernández, Emérita Del Carmen Pons, Delfina Rueda, Odalis Teresa Sinisterra, Enrique Murillo, Marilyn E Scott, Kristine G Koski
BACKGROUND: The usefulness of C-reactive protein (CRP) as a non-specific marker of inflammation during pregnancy and lactation is unclear in impoverished populations where co-existing infections and vitamin deficiencies are common. METHODS: This cross-sectional study in Panama recruited 120 pregnant and 99 lactating Ngäbe-Buglé women from 14 communities in rural Panama. Obstetric history, indoor wood smoke exposure, fieldwork, BMI, vitamins A, B12, D, and folic acid, and inflammation markers (CRP, neutrophil/lymphocyte ratio (NLR), plateletcrit and cytokines) were measured...
June 2, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28570610/bacterial-community-structure-transformed-after-thermophilically-composting-human-waste-in-haiti
#13
Yvette M Piceno, Gabrielle Pecora-Black, Sasha Kramer, Monika Roy, Francine C Reid, Eric A Dubinsky, Gary L Andersen
Recycling human waste for beneficial use has been practiced for millennia. Aerobic (thermophilic) composting of sewage sludge has been shown to reduce populations of opportunistically pathogenic bacteria and to inactivate both Ascaris eggs and culturable Escherichia coli in raw waste, but there is still a question about the fate of most fecal bacteria when raw material is composted directly. This study undertook a comprehensive microbial community analysis of composting material at various stages collected over 6 months at two composting facilities in Haiti...
2017: PloS One
https://www.readbyqxmd.com/read/28567041/pathogen-reactive-t-helper-cell-analysis-in-the-pig
#14
Friederike Ebner, Patrycja Schwiertz, Svenja Steinfelder, Robert Pieper, Jürgen Zentek, Nicole Schütze, Christoph G Baums, Gottfried Alber, Peter Geldhof, Susanne Hartmann
There is growing interest in studying host-pathogen interactions in human-relevant large animal models such as the pig. Despite the progress in developing immunological reagents for porcine T cell research, there is an urgent need to directly assess pathogen-specific T cells-an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. Here, we established that the activation marker CD154 (CD40L), known from human and mouse studies, identifies also porcine antigen-reactive CD4(+) T lymphocytes...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28562805/high-prevalence-of-enteroparasites-in-children-from-ribeir%C3%A3-o-preto-s%C3%A3-o-paulo-brazil
#15
Renata Elizabete Pagotti da Fonseca, Michelle Christiane Rodrigues Barbosa, Beatriz Rossetti Ferreira
Objective: To determine the prevalence of intestinal parasites and possible associated factors. Method: Cross-sectional epidemiological study of 962 children (3-12-years) from an area within the scope of a basic health unit in Ribeirão Preto, São Paulo, Brazil. Data were collected by home interviews and three-sample stool analysis and evaluated with the chi-square and Fisher's exact tests (p<0.05). Results: The prevalence of intestinal parasites was significantly high (57...
May 2017: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/28560181/a-modified-assay-for-the-enumeration-of-ascaris-eggs-in-fresh-raw-sewage
#16
Esmaeil Shahsavari, Jonathan Schmidt, Arturo Aburto-Medina, Basma Khallaf, Vivek Balakrishnan, Nicholas D Crosbie, Aravind Surapaneni, Andrew S Ball
Soil-transmitted helminths (STHs) pose a significant public health problem, infecting approximately 2 billion people globally. Despite relatively low prevalence in developed countries, the removal of STHs from wastewater remains crucial to allow the safe use of biosolids or recycled water for agriculture. Wastewater helminth egg count data can contribute to an assessment of the need for, or success of, a parasite management program. Although the World Health Organisation (WHO) has recommended a standard method for counting helminth eggs in raw sewage based on the method of Bailenger (Ayres et al...
2017: MethodsX
https://www.readbyqxmd.com/read/28545561/sources-of-variability-in-the-measurement-of-ascaris-lumbricoides-infection-intensity-by-kato-katz-and-qpcr
#17
Alice V Easton, Rita G Oliveira, Martin Walker, Elise M O'Connell, Sammy M Njenga, Charles S Mwandawiro, Joanne P Webster, Thomas B Nutman, Roy M Anderson
BACKGROUND: Understanding and quantifying the sources and implications of error in the measurement of helminth egg intensity using Kato-Katz (KK) and the newly emerging "gold standard" quantitative polymerase chain reaction (qPCR) technique is necessary for the appropriate design of epidemiological studies, including impact assessments for deworming programs. METHODS: Repeated measurements of Ascaris lumbricoides infection intensity were made from samples collected in western Kenya using the qPCR and KK techniques...
May 25, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28542260/hookworm-infection-is-associated-with-decreased-cd4-t-cell-counts-in-hiv-infected-adult-ugandans
#18
Bozena M Morawski, Miya Yunus, Emmanuel Kerukadho, Grace Turyasingura, Logose Barbra, Andrew Mijumbi Ojok, Andrew R DiNardo, Stefanie Sowinski, David R Boulware, Rojelio Mejia
Most studies evaluating epidemiologic relationships between helminths and HIV have been conducted in the pre-ART era, and evidence of the impact of helminth infections on HIV disease progression remains conflicting. Less is known about helminth infection and clinical outcomes in HIV-infected adults receiving antiretroviral therapy (ART). We sampled HIV-infected adults for eight gastrointestinal parasites and correlated parasitic infection with demographic predictors, and clinical and immunologic outcomes. Contrasting with previous studies, we measured parasitic infection with a quantitative, highly sensitive and specific polymerase chain reaction (PCR) method...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28531364/analysis-of-the-internal-transcribed-spacer-region-of-ascaris-suum-and-ascaris-lumbricoides-derived-from-free-range-tibetan-pigs
#19
Kun Li, Houqiang Luo, Hui Zhang, Khalid Mehmood, Muhammad Shahzad, Lihong Zhang, Jiakui Li
Ascaris suum (A. suum) is the most commonly occurring worldwide internal parasite of pigs; however, little is known about this organism in Tibetan pigs in China. A study was carried to isolate and identify the characteristics of internal transcribed spacer region (ITS) gene of A. suum derived from Tibetan pigs. Adult nematodes were collected from Tibetan pigs in 2015-2016. Total genomic DNA of the extracted parasites was performed and a fragment of the ITS of mitochondrial (mt) gene was amplified. The amplicons were cloned into PGEM®-T Easy Vector (Promega, WI) and the positive clones were sequenced by ABI 3730 × 1 sequencer...
May 21, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28507618/correlation-between-soil-transmitted-helminth-infection-and-eosinophil-levels-among-primary-school-children-in-medan
#20
Dewi Masyithah Darlan, Zaimah Z Tala, Cellya Amanta, Syah Mirsya Warli, Nurfida K Arrasyid
BACKGROUND: Soil Transmitted Helminth infection is one of most prevalent health problems worldwide, especially in environments with poor sanitation. Based on World Health Organisation (WHO) data, more than 2 billion people, or 24% of the world's population, are infected with intestinal parasite. The highest prevalence is located in areas of poor sanitation and unsafe water supplies. In Indonesia, the prevalence of parasite infections is 15% of the entire population. AIM: The purpose of this study was to determine the relationship between Soil Transmitted Helminth infection on levels of eosinophils among primary school children...
April 15, 2017: Open Access Macedonian Journal of Medical Sciences
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