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https://www.readbyqxmd.com/read/28931127/single-visit-rabies-pre-exposure-priming-induces-a-robust-anamnestic-antibody-response-after-simulated-post-exposure-vaccination-results-of-a-dose-finding-study
#1
Emile F F Jonker, Leonardus G Visser
Background: The current standard 3-dose intramuscular rabies PrEP schedule suffers from a number of disadvantages that severely limit accessibility and availability. The cost of is often prohibitive, it requires 3 visits to the clinic, and there are regular vaccine shortages. Methods: Volunteers ( N  = 30) were randomly assigned to 4 study arms: 1 standard dose intramuscular (IM) dose of PVRV (purified Vero cell rabies vaccine, Verorab), and 1/5th, 2/5th or 3/5th- fractional intradermal (ID) dose of PVRV in a single visit...
September 1, 2017: Journal of Travel Medicine
https://www.readbyqxmd.com/read/28927795/synthesis-and-evaluation-of-the-nsclc-anti-cancer-activity-and-physical-properties-of-4-aryl-n-phenylpyrimidin-2-amines
#2
Borvornwat Toviwek, Praphasri Suphakun, Kiattawee Choowongkomon, Supa Hannongbua, M Paul Gleeson
Reported herein are efforts to profile 4-aryl-N-phenylpyrimidin-2-amines in terms of their anti-cancer activity towards non small-cell lung carcinoma (NSCLC) cells. We have synthesized new 4-aryl-N-phenylpyrimidin-2-amines and assessed them in terms of their cytotoxicity (A549, NCI-H187, MCF7, Vero & KB) and physicochemical properties (logD7.4 and solubility). 13f and 13c demonstrated potent anti-cancer activity in A549 cells (0.2µM), compared to 0.4μM for the NSCLC drug Doxorubicin. 13f also displayed low experimental logD7...
September 1, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28925873/silver-nanoparticles-with-high-loading-capacity-of-amphotericin-b-characterization-bactericidal-and-antifungal-effects
#3
Victoria Leonhard, Roxana Valeria Alasino, Adrián Muñoz, Dante Miguel Beltramo
The purpose of this study was to evaluate the most appropriate conditions to generate silver nanoparticles (AgNPs) loaded with a potent antimycotic drug like amphotericin B (AmB), characterize the physicochemical properties, and also evaluate the cytotoxic effect and biological activity of these new nanostructures as a potential nanocarrier for hydrophobic drugs. It was determined that the optimal molar ratio between Ag and AmB is 1/1 given the uniformity of size around 1750 nm of the nanoparticles generated as well as their strongly negative ζ potential of -27 mV, a condition that favors repulsions between AgNPs and inhibiting their aggregation...
September 18, 2017: Current Drug Delivery
https://www.readbyqxmd.com/read/28912011/cell-line-dependent-antiviral-activity-of-sofosbuvir-against-zika-virus
#4
Noreen Mumtaz, Leah C Jimmerson, Lane R Bushman, Jennifer J Kiser, Georgina Aron, Chantal B E M Reusken, Marion P G Koopmans, Jeroen J A van Kampen
The recent epidemic of Zika virus (ZIKV) in the Americas and its association with fetal and neurological complications has shown the need to develop a treatment. Repurposing of drugs that are already FDA approved or in clinical development may shorten drug development timelines in case of emerging viral diseases like ZIKV. Initial studies have shown conflicting results when testing sofosbuvir developed for treatment of infections with another Flaviviridae virus, hepatitis C virus. We hypothesized that the conflicting results could be explained by differences in intracellular processing of the compound...
September 11, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28906457/antibacterial-properties-of-flavonoids-from-kino-of-the-eucalypt-tree-corymbia-torelliana
#5
Motahareh Nobakht, Stephen J Trueman, Helen M Wallace, Peter R Brooks, Klrissa J Streeter, Mohammad Katouli
Traditional medicine and ecological cues can both help to reveal bioactive natural compounds. Indigenous Australians have long used kino from trunks of the eucalypt tree, Corymbia citriodora, in traditional medicine. A closely related eucalypt, C. torelliana, produces a fruit resin with antimicrobial properties that is highly attractive to stingless bees. We tested the antimicrobial activity of extracts from kino of C. citriodora, C. torelliana × C. citriodora, and C. torelliana against three Gram-negative and two Gram-positive bacteria and the unicellular fungus, Candida albicans...
September 14, 2017: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28904193/mutations-in-the-fusion-protein-of-measles-virus-that-confer-resistance-to-the-membrane-fusion-inhibitors-carbobenzoxy-d-phe-l-phe-gly-and-as-48
#6
Michael N Ha, Sébastien Delpeut, Ryan S Noyce, Gary Sisson, Karen M Black, Liang-Tzung Lin, Darius Bilimoria, Richard K Plemper, Gilbert G Privé, Christopher D Richardson
The inhibitors Z-d-Phe-l-Phe Gly (fusion inhibitor peptide, FIP) and AS-48 have similar efficacy in blocking membrane fusion and syncytia formation mediated by measles virus. Other homologues such as Z-d-Phe are less effective, but may act through the same mechanism. In an attempt to map the site of action of these inhibitors, we generated mutant viruses which were resistant to the inhibitory effects of Z-d-Phe-l-Phe Gly. These 10 mutations were localized to the heptad repeat region (HRB) of the fusion protein and no changes were observed in the viral hemagglutinin, which is the receptor attachment protein...
September 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28898286/structural-investigation-of-c6-36-and-vero-cell-cultures-infected-with-a-brazilian-zika-virus
#7
Debora Ferreira Barreto-Vieira, Fernanda Cunha Jácome, Marcos Alexandre Nunes da Silva, Gabriela Cardoso Caldas, Ana Maria Bispo de Filippis, Patrícia Carvalho de Sequeira, Elen Mello de Souza, Audrien Alves Andrade, Pedro Paulo de Abreu Manso, Gisela Freitas Trindade, Sheila Maria Barbosa Lima, Ortrud Monika Barth
Zika virus (ZIKV) is a member of the flavivirus genus, and its genome is approximately 10.8 kilobases of positive-strand RNA enclosed in a capsid and surrounded by a membrane. Studies on the replication dynamics of ZIKV are scarce, which limits the development of antiviral agents and vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) and African green monkey kidney epithelial cells (Vero cells) were inoculated with a ZIKV sample isolated from a Brazilian patient, and the infection was characterized by immunofluorescence staining, phase contrast light microscopy, transmission electron microscopy and real-time RT-PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28895925/bovine-lactoferrin-inhibits-dengue-virus-infectivity-by-interacting-with-heparan-sulfate-low-density-lipoprotein-receptor-and-dc-sign
#8
Jo-Mei Chen, Yi-Chin Fan, Jen-Wei Lin, Yi-Ying Chen, Wei-Li Hsu, Shyan-Song Chiou
Bovine lactoferrin (bLF) presents in milk and has been shown to inhibit several viral infections. Effective drugs are unavailable for the treatment of dengue virus (DENV) infection. In this study, we evaluated the antiviral effect of bLF against DENV infection in vivo and in vitro. Bovine LF significantly inhibited the infection of the four serotypes of DENV in Vero cells. In the time-of-drug addition test, DENV-2 infection was remarkably inhibited when bLF was added during or prior to the occurrence of virus attachment...
September 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28892514/l-thyroxine-modifies-nephrotoxicity-by-regulating-the-apoptotic-pathway-the-possible-role-of-cd38-adp-ribosyl-cyclase-mediated-calcium-mobilization
#9
Tarek El-Hamoly, Dina M El-Sharawy, Marwa S El Refaye, Sahar S Abd El-Rahman
Thyroid hormones are well-established as a key regulator of many cellular metabolic pathways developed in various pathogeneses. Here, we dedicated the current work to investigate the role of thyroid hormone analogue (L-thyroxine, L-TH) in regulating the renal cytotoxicity using in vivo and in vitro models. Swiss mice were exposed to gamma radiation (IRR, 6Gy) or treated with cisplatin (CIS, 15 mg/kg, i.p.) for induction of nephrotoxicity. Remarkably, pretreatment with L-TH (1μg/kg) ameliorated the elevated kidney function biomarkers, oxidative stress and protected the renal tissue from the subsequent cellular damage...
2017: PloS One
https://www.readbyqxmd.com/read/28886313/susceptibility-of-herpes-simplex-virus-type-1-to-monoterpenes-thymol-carvacrol-p-cymene-and-essential-oils-of-sinapis-arvensis-l-lallemantia-royleana-benth-and-pulicaria-vulgaris-gaertn
#10
J Sharifi-Rad, B Salehi, P Schnitzler, S A Ayatollahi, F Kobarfard, M Fathi, M Eisazadeh, M Sharifi-Rad
In recent years, with increased the prevalence of viral infections and having no specific for  their treatment  and also the continuous appearance of resistant viral strains, the finding of novel antiviral agents is necessary. In this study, monoterpenes of thymol, carvacrol, p-cymene and essential oils from Sinapis arvensis L., Lallemantia royleana Benth. and Pulicaria vulgaris Gaertn. were screened for their inhibitory effect against herpes simplex virus type 1 (HSV-1) in vitro on Vero cell line CCL-81-ATCC using a plaque reduction assay...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28880112/coumarins-and-alkaloids-from-the-roots-of-toddalia-asiatica
#11
Sanwat Sukieum, Wichien Sang-Aroon, Chavi Yenjai
The EtOAc and MeOH extracts of the roots of Toddalia asiatica Lam. were investigated for the roots' chemical constituents. Two new compounds including 2'R-acetoxytoddanol (1) and 8S-10-O-demethylbocconoline (3) as well as 15 known compounds were isolated. Compound 10 showed strong cytotoxicity against KB cells with an IC50 value of 2.60 μg/mL, which is nearly equal to the ellipticine standard, but showed no activity against Vero cells. Alkaloid 3 displayed weak cytotoxicity against the KB cell line with an IC50 value of 21...
September 7, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28874468/tim1-havcr1-is-not-essential-for-cellular-entry-of-either-quasi-enveloped-or-naked-hepatitis-a-virions
#12
Anshuman Das, Asuka Hirai-Yuki, Olga González-López, Bethany Rhein, Sven Moller-Tank, Rachel Brouillette, Lucinda Hensley, Ichiro Misumi, William Lovell, John M Cullen, Jason K Whitmire, Wendy Maury, Stanley M Lemon
Receptor molecules play key roles in the cellular entry of picornaviruses, and TIM1 (HAVCR1) is widely accepted to be the receptor for hepatitis A virus (HAV), an unusual, hepatotropic human picornavirus. However, its identification as the hepatovirus receptor predated the discovery that hepatoviruses undergo nonlytic release from infected cells as membrane-cloaked, quasi-enveloped HAV (eHAV) virions that enter cells via a pathway distinct from naked, nonenveloped virions. We thus revisited the role of TIM1 in hepatovirus entry, examining both adherence and infection/replication in cells with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-engineered TIM1 knockout...
September 5, 2017: MBio
https://www.readbyqxmd.com/read/28870343/solvent-solvent-fractionations-of-combretum-erythrophyllum-burch-leave-extract-studies-of-their-antibacterial-antifungal-antioxidant-and-cytotoxicity-potentials
#13
Fanyana M Mtunzi, Ikechukwu P Ejidike, Imelda Ledwaba, Aroke Ahmed, Vusumzi E Pakade, Michael J Klink, Sekomeng J Modise
OBJECTIVE: To evaluate the biological activities of Combretum erythrophyllum (C. erythrophyllum) leaf extracts against infectious diseases' pathogenesis and their cytotoxicity potentials. METHODS: Powdered leaf material (300 g) of C. erythrophyllum was extracted (1:10 w/v) using acetone to obtain the crude extract. Liquid-liquid fractionation was performed on the crude acetone extract (30 g) using solvents of different polarity. The bioautographic method was used to detect the inhibition of bacterial and fungal growth by active compounds present in the crude and fractions...
July 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28865682/purification-of-trypanosoma-cruzi-metacyclic-trypomastigotes-by-ion-exchange-chromatography-in-sepharose-deae-a-novel-methodology-for-host-pathogen-interaction-studies
#14
Lissa Cruz-Saavedra, Marina Muñoz, Cielo León, Manuel Alfonso Patarroyo, Gabriela Arevalo, Paula Pavia, Gustavo Vallejo, Julio César Carranza, Juan David Ramírez
Metacyclic trypomastigotes are essential for the understanding of the biology of Trypanosoma cruzi, the agent of Chagas disease. However, obtaining these biological stages in axenic medium is difficult. Techniques based on charge and density of the parasite during different stages have been implemented, without showing a high efficiency in the purification of metacyclic trypomastigotes. So far, there is no protocol implemented where sepharose-DEAE is used as a resin. Therefore, herein we tested its ability to purify metacyclic trypomastigotes in Liver Infusion Triptose (LIT) medium cultures...
September 1, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28861621/enteroviruses-in-blood-of-patients-with-type-1-diabetes-detected-by-integrated-cell-culture-and-reverse-transcription-quantitative-real-time-pcr
#15
Enagnon Kazali Alidjinou, Famara Sane, Christine Lefevre, Agathe Baras, Ilham Moumna, Ilka Engelmann, Marie-Christine Vantyghem, Didier Hober
AIMS: Enteroviruses (EV) have been associated with type 1 diabetes (T1D), but EV RNA detection has been reported in only a small proportion of T1D patients. We studied whether integrated cell culture and reverse transcription real-time PCR could improve EV detection in blood samples from patients with T1D. METHODS: Blood was collected from 13 patients with T1D. The presence of EV RNA in blood was investigated by using real-time RT-PCR. In addition, plasma and white blood cells (WBC) were inoculated to BGM and Vero cell line cultures...
August 31, 2017: Acta Diabetologica
https://www.readbyqxmd.com/read/28854955/identification-of-two-mutation-sites-in-spike-and-envelope-proteins-mediating-optimal-cellular-infection-of-porcine-epidemic-diarrhea-virus-from-different-pathways
#16
Min Sun, Jiale Ma, Zeyanqiu Yu, Zihao Pan, Chengping Lu, Huochun Yao
Entry of the α-coronavirus porcine epidemic diarrhea virus (PEDV) requires specific proteases to activate spike (S) protein for the membrane fusion of the virion to the host cell following receptor binding. Herein, PEDV isolate 85-7 could proliferate and induce cell-cell fusion in a trypsin independent manner on Vero cells, and eight homologous mutation strains were screened by continuous proliferation in the absence of trypsin on Vero cells. According to the whole genome sequence comparative analysis, we identified four major variations located in nonstructural protein 2, S, open reading frame 3, and envelope (E) genes, respectively...
August 30, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28844400/meso-dihydroguaiaretic-acid-derivatives-with-antibacterial-and-antimycobacterial-activity
#17
Karen Reyes-Melo, Abraham García, Antonio Romo-Mancillas, Elvira Garza-González, Verónica M Rivas-Galindo, Luis D Miranda, Javier Vargas-Villarreal, Juan Manuel J Favela-Hernández, María Del Rayo Camacho-Corona
Thirty-three meso-dihydroguaiaretic acid (meso-DGA) derivatives bearing esters, ethers, and amino-ethers were synthesized. All derivatives were tested against twelve drug-resistant clinical isolates of Gram-positive and Gram-negative bacteria, including sensitive (H37Rv) and multidrug-resistant Mycobacterium tuberculosis strains. Among the tested compounds, four esters (7, 11, 13, and 17), one ether (23), and three amino-ethers (30, 31, and 33) exhibited moderate activity against methicillin-resistant Staphylococcus aureus, whereas 30 and 31 showed better results than levofloxacin against vancomycin-resistant Enterococcus faecium...
July 29, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28844141/discovery-and-optimization-of-5-amino-1-2-3-triazole-4-carboxamide-series-against-trypanosoma-cruzi
#18
Stephen Brand, Eun Jung Ko, Elisabet Viayna, Stephen Thompson, Daniel Spinks, Michael Thomas, Lars Sandberg, Amanda F Francisco, Shiromani Jayawardhana, Victoria C Smith, Chimed Jansen, Manu De Rycker, John Thomas, Lorna MacLean, Maria Osuna-Cabello, Jennifer Riley, Paul Scullion, Laste Stojanovski, Frederick R C Simeons, Ola Epemolu, Yoko Shishikura, Sabrinia D Crouch, Tania S Bakshi, Christopher J Nixon, Iain H Reid, Alan P Hill, Tim Z Underwood, Sean J Hindley, Sharon A Robinson, John M Kelly, Jose M Fiandor, Paul G Wyatt, Maria Marco, Timothy J Miles, Kevin D Read, Ian H Gilbert
Chagas' disease, caused by the protozoan parasite Trypanosoma cruzi, is the most common cause of cardiac-related deaths in endemic regions of Latin America. There is an urgent need for new safer treatments because current standard therapeutic options, benznidazole and nifurtimox, have significant side effects and are only effective in the acute phase of the infection with limited efficacy in the chronic phase. Phenotypic high content screening against the intracellular parasite in infected VERO cells was used to identify a novel hit series of 5-amino-1,2,3-triazole-4-carboxamides (ATC)...
September 14, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28843818/perillyl-alcohol-exhibits-in-vitro-inhibitory-activity-against-plasmodium-falciparum-and-protects-against-experimental-cerebral-malaria
#19
Adriana A Marin Rodriguez, Leonardo J M Carvalho, Emilia A Kimura, Alejandro M Katzin
The development of new drugs is one of the strategies to control malaria. Isoprenoid biosynthesis in Plasmodium falciparum is an essential pathway for parasite survival, and therefore a potential target for new antimalarial drugs. Indeed, plant-derived secondary metabolites such as terpenes exhibit antimalarial activity in vitro by inhibiting isoprenoid biosynthesis in P. falciparum. In this study, the in vitro antiplasmodial activity of perillyl alcohol (POH) was evaluated, along with its in vitro toxicity and its effect on the isoprenylation process...
August 23, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28841362/use-of-the-live-attenuated-japanese-encephalitis-vaccine-sa-14-14-2-in-children-a-review-of-safety-and-tolerability-studies
#20
Amy Sarah Ginsburg, Ankita Meghani, Scott B Halstead, Mansour Yaich
Japanese encephalitis (JE) is the leading cause of viral neurological disease and disability in Asia. Some 50-80% of children with clinical JE die or have long-term neurologic sequelae. Since there is no cure, human vaccination is the only effective long-term control measure, and the World Health Organization recommends that at-risk populations receive a safe and effective vaccine. Four different types of JE vaccines are currently available: inactivated mouse brain-derived vaccines, inactivated Vero cell vaccines, live attenuated SA 14-14-2 vaccines and a live recombinant (chimeric) vaccine...
August 25, 2017: Human Vaccines & Immunotherapeutics
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