keyword
MENU ▼
Read by QxMD icon Read
search

Vero

keyword
https://www.readbyqxmd.com/read/28641157/design-synthesis-and-structure-activity-relationship-studies-of-a-novel-focused-library-of-2-3-4-substituted-oxazolidines-with-antiproliferative-activity-against-cancer-cell-lines
#1
Saulo F Andrade, Bárbara G Oliveira, Larissa C Pereira, Jonas P Ramos, Angélica R Joaquim, Martin Steppe, Elaine M Souza-Fagundes, Ricardo J Alves
In the present work we describe the synthesis and antiproliferative evaluation of a focused library of 30 novel oxazolidines designed by modification of N-substituent, by ring variation, by alkyl variation or by extension of the structure. It was noted that carbamate and N,O-aminal groups were essential for activity. In general, replacement of the phenyl ring with pyridinyl was not tolerated. However, the introduction of a second phenyl ring with an appropriate spacer at the 3- or 4-position of the first phenyl ring generally enhanced the cytotoxic profile...
June 12, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28638876/phytochemical-screening-antimicrobial-and-cytotoxicity-studies-of-ethanol-leaf-extract-of-aphaniasenegalensis-sapindaceae
#2
Alioune Dior Fall, Victor Patrick Bagla, Emmanuel Bassene, Jacobus Nicolaas Eloff
BACKGROUND: Aphania senegalensis (Sapindaceae) is commonly used in Senegalese traditional medicine to treat pain, inflammation, asthenia, bacterial and fungal infections. The aim of this study was to determine the type of phytochemical constituents present in the ethanol leaf extract and its antimicrobial activity against selected bacterial and fungal pathogens. MATERIALS AND METHODS: The ethanol leaf extract of A. senegalensis was evaluated for its cytotoxic effect in the MTT assay against Vero cells...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28629354/acetone-leaf-extracts-of-some-south-african-trees-with-high-activity-against-escherichia-coli-also-have-good-antimycobacterial-activity-and-selectivity-index
#3
Ishaku L Elisha, Francien S Botha, Balungile Madikizela, Lyndy J McGaw, Jacobus N Eloff
BACKGROUND: Tuberculosis is a world-wide problem affecting humans and animals. There is increasing development of resistance of the pathogens to current antimycobacterial agents. Many authors have investigated activities of extracts and isolated compounds from plants. The traditional uses of plants have frequently been the criterion to select plants investigated. In this contribution, we investigate whether plant extracts with very good activity against Escherichia coli may also be active against mycobacteria...
June 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28629039/time-dependent-subcellular-structure-injuries-induced-by-nano-micron-sized-calcium-oxalate-monohydrate-and-dihydrate-crystals
#4
Xin-Yuan Sun, Kai Yu, Jian-Ming Ouyang
Comparative studies were conducted to investigate the time effect of cell injury induced by nano-sized (50nm) and micron-sized (10μm) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals in African green monkey renal epithelial (Vero) cells. The effects of nano-/micron-sized COM and COD exposure on Vero cells were investigated by detecting the cell viability, cell morphology, LDH release, reactive oxygen species, mitochondrial membrane potential, cell cycle, and cell apoptosis, as well as the intracellular and extracellular crystal distribution...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627357/the-heat-shock-protein-90-of-toxoplasma-gondii-is-essential-for-invasion-of-host-cells-and-tachyzoite-growth
#5
Hongchao Sun, Xunhui Zhuo, Xianfeng Zhao, Yi Yang, Xueqiu Chen, Chaoqun Yao, Aifang Du
Toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects almost all warm-blooded vertebrates. Heat shock proteins (HSP) regulate key signal transduction events in many organisms, and heat shock protein 90 (Hsp90) plays an important role in growth, development, and virulence in several parasitic protozoa. Here, we discovered increased transcription of the Hsp90 gene under conditions for bradyzoite differentiation, i.e. alkaline and heat shock conditions in vitro, suggesting that Hsp90 may be connected with bradyzoite development in T...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/28612010/recombinant-swinepox-virus-expressing-glycoprotein-e2-of-classical-swine-fever-virus-confers-complete-protection-in-pigs-upon-viral-challenge
#6
Huixing Lin, Zhe Ma, Lei Chen, Hongjie Fan
Classical swine fever (CSF) is a highly contagious and serious viral disease that affects the pig industry worldwide. The glycoprotein E2 of the classical swine fever virus (CSFV) can induce neutralizing antibodies, and it is widely used for novel vaccine development. To explore the development of a vaccine against CSFV infections, the gene of glycoprotein E2 was inserted into the swinepox virus (SPV) genome by homologous recombination. The culture titers of rSPV-E2 remained at about 4.3 × 10(6) TCID50 for more than 60 passages in PK15 and swine testis cell lines...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28602767/an-easily-transfectable-cell-line-that-produces-an-infectious-reporter-virus-for-routine-and-robust-quantitation-of-kaposi-s-sarcoma-associated-herpesvirus-reactivation
#7
Jennifer L DeCotiis, Noelle C Ortiz, Brian A Vega, David M Lukac
Reactivation of Kaposi's sarcoma-associated herpesvirus (KHSV; also known as Human herpesvirus (HHV)-8) from latency is associated with progression to disease. The primary experimental models for studying KSHV reactivation are B lymphocyte cell lines derived from patients with primary effusion lymphoma (PEL). PEL models have remained essential tools for understanding molecular details of latency and reactivation, yet they have shortcomings. In particular, PEL cells are difficult to transfect with plasmid DNA, which limits their routine use in studies that require introduction of exogenous DNA...
June 8, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28599659/zika-vector-transmission-risk-in-temperate-australia-a-vector-competence-study
#8
Jean-Bernard Duchemin, Peter T Mee, Stacey E Lynch, Ravikiran Vedururu, Lee Trinidad, Prasad Paradkar
BACKGROUND: Zika virus is an emerging pathogen of global importance. It has been responsible for recent outbreaks in the Americas and in the Pacific region. This study assessed five different mosquito species from the temperate climatic zone in Australia and included Aedes albopictus as a potentially invasive species. METHODS: Mosquitoes were orally challenged by membrane feeding with Zika virus strain of Cambodia 2010 origin, belonging to the Asian clade. Virus infection and dissemination were assessed by quantitative PCR on midgut and carcass after dissection...
June 9, 2017: Virology Journal
https://www.readbyqxmd.com/read/28596029/2-phenylnaphthalenes-and-a-polyoxygenated-cyclohexene-from-the-stem-and-root-extracts-of-uvaria-cherrevensis-annonaceae
#9
Chiramet Auranwiwat, Puttandon Wongsomboon, Thanaphat Thaima, Roonglawan Rattanajak, Sumalee Kamchonwongpaisan, Anthony C Willis, Wilford Lie, Stephen G Pyne, Thunwadee Limtharakul Née Ritthiwigrom
Three new 2-phenylnaphthalene derivatives, cherrevenaphthalenes A-C (1-3), and a new polyoxygenated cyclohexene derivative, (-)-uvaribonol F (4) together with six known compounds, 5-10, were isolated from the stem and root extracts of Uvaria cherrevensis (Annonaceae). The structures of all isolated compounds were elucidated by spectroscopic analysis. The structures of 3 and 4 were further confirmed by single crystal X-ray diffraction methods. Compound 2 exhibited modest antiplasmodial activity against the P...
June 9, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28595631/isolation-of-tibet-orbivirus-from-culicoides-and-associated-infections-in-livestock-in-yunnan-china
#10
Jinglin Wang, Huachun Li, Yuwen He, Yang Zhou, Aiguo Xin, Defang Liao, Jinxin Meng
BACKGROUND: Culicoides-borne orbiviruses, such as bluetongue virus (BTV) and African horse sickness virus (AHSV), are important pathogens that cause animal epidemic diseases leading to significant loss of domestic animals. This study was conducted to identify Culicoides-borne arboviruses and to investigate the associated infections in local livestock in Yunnan, China. METHODS: Culicoides were collected overnight in Mangshi City using light traps during August 2013...
June 8, 2017: Virology Journal
https://www.readbyqxmd.com/read/28586726/evaluation-of-cytotoxicity-of-hematite-nanoparticles-in-bacteria-and-human-cell-lines
#11
Kumar Rajendran, Shampa Sen, Suja G, S Lakshmana Senthil, T Vinoth Kumar
Metal nanoparticles have a great impact and even change the composition of soil microbial communities. This poses the risk of their accumulation in the ecosystem, which may call on health hazard. Statistical techniques such as Plackett-Burman design, Response Surface Methodology were used for optimizing medium constituents for Bacillus cereus SVK1 and other critical variables responsible for the production of biomolecules and biosynthesis of hematite nanoparticles. The effect of hematite nanoparticles on the growth of soil bacteria were tested by agar-well diffusion method and dynamic growth curve techniques...
May 22, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28585287/effect-of-vero-pan-tilt-motion-on-the-dose-distribution
#12
Heru Prasetio, Indra Yohannes, Christoph Bert
Tumor tracking is an option for intra-fractional motion management in radiotherapy. The VERO gimbal tracking system creates a unique beam geometry and understanding the effect of the gimbal motion in terms of dose distribution is important to assess the dose deviation from the reference conditions. Beam profiles, output factors (OF) and percentage depth doses (PDD) were measured and evaluated to investigate this effect. In order to find regions affected by the pan-tilt motion, synthesized 2D dose distributions were generated...
June 6, 2017: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28582669/qsar-driven-design-synthesis-and-discovery-of-potent-chalcone-derivatives-with-antitubercular-activity
#13
Marcelo N Gomes, Rodolpho C Braga, Edyta M Grzelak, Bruno J Neves, Eugene Muratov, Rui Ma, Larry L Klein, Sanghyun Cho, Guilherme R Oliveira, Scott G Franzblau, Carolina Horta Andrade
New anti-tuberculosis (anti-TB) drugs are urgently needed to battle drug-resistant Mycobacterium tuberculosis strains and to shorten the current 6-12-month treatment regimen. In this work, we have continued the efforts to develop chalcone-based anti-TB compounds by using an in silico design and QSAR-driven approach. Initially, we developed SAR rules and binary QSAR models using literature data for targeted design of new heteroaryl chalcone compounds with anti-TB activity. Using these models, we prioritized 33 compounds for synthesis and biological evaluation...
May 10, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28581345/development-and-identification-of-a-new-vero-cell-based-live-attenuated-influenza-b-vaccine-by-a-modified-classical-reassortment-method
#14
Fan Yang, Lei Ma, Jian Zhou, Yinjie Wu, Jingxia Gao, Shaohui Song, Xingliang Geng, Qi Guo, Zhuofan Li, Weidong Li, Guoyang Liao
OBJECTIVE: The goal of this study was to generate a new Vero and cold-adapted live attenuated influenza B vaccine and evaluate its safety and immunogenicity with experiments in a guinea pig model. METHODS: A modified classical reassortment method was used to generate the new vaccine strain. The donor strain was B/Yunnan/2/2005Vca(B), and the parental virus strain was B/Brisbane/60/2008wt. After co-infection in Vero cells, the prepared antibody serum inhibited the donor strain growth, and screening conditions inhibited the candidate virus growth, which induced the growth of the new reassortant virus B/Brisbane/60/2008Vca(B) grow...
June 5, 2017: Expert Review of Vaccines
https://www.readbyqxmd.com/read/28578573/antifungal-and-antiviral-cyclic-peptides-from-the-deep-sea-derived-fungus-simplicillium-obclavatum-eiodsf-020
#15
Xiao Liang, Xu-Hua Nong, Zhong-Hui Huang, Shu-Hua Qi
A new linear peptide simplicilliumtide I (1) and four new cyclic peptides simplicilliumtides J-M (2-5) together with known analogues verlamelins A and B (6 and 7) were isolated from the deep-sea-derived fungal strain Simplicillium obclavatum EIODSF 020. Their structures were elucidated by spectroscopic analysis, and their absolute configurations were further confirmed by chemical structural modification, Marfey's and Mosher's methods. Compounds 2, 6, and 7 showed significant antifungal activity toward Aspergillus versicolor and Curvularia australiensis and also had obvious antiviral activity toward HSV-1 with IC50 values of 14...
June 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28577368/inhibitors-of-ubiquitin-e3-ligase-as-potential-new-antimalarial-drug-leads
#16
Jagrati Jain, Surendra K Jain, Larry A Walker, Babu L Tekwani
BACKGROUND: Protein ubiquitylation is an important post-translational regulation, which has been shown to be necessary for life cycle progression and survival of Plasmodium falciparum. Ubiquitin is a highly conserved 76 amino acid polypeptide, which attaches covalently to target proteins through combined action of three classes of enzymes namely, the ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin-protein ligase (E3). Ubiquitin E1 and E2 are highly conserved within eukaryotes...
June 2, 2017: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28576364/lack-of-viable-parasites-in-cured-parma-ham-pdo-following-experimental-toxoplasma-gondii-infection-of-pigs
#17
Marco Genchi, Alice Vismarra, Carlo Mangia, Silvia Faccini, Nadia Vicari, Sara Rigamonti, Paola Prati, Anna Maria Marino, Laura Kramer, Massimo Fabbi
Twelve Large White pigs were experimentally infected with 1000 Toxoplasma gondii oocysts/each. Serology was carried out at different time points post infection (p.i.) and animals were slaughtered at four months p.i. One of two thighs was examined for T. gondii infection status by PCR and bioassay in mice. The other thigh was processed for Parma ham production. Four thighs were examined after twelve months of curing, four after fourteen months and four were examined after sixteen months. Cured hams were analyzed by PCR, bioassay and in-vitro cultivation on Vero cells followed by real-time PCR...
September 2017: Food Microbiology
https://www.readbyqxmd.com/read/28571695/influenza-viruses-production-evaluation-of-a-novel-avian-cell-line-duckcelt%C3%A2-t17
#18
Emma Petiot, Anaïs Proust, Aurélien Traversier, Laurent Durous, Frédéric Dappozze, Marianne Gras, Chantal Guillard, Jean-Marc Balloul, Manuel Rosa-Calatrava
The influenza vaccine manufacturing industry is looking for production cell lines that are easily scalable, highly permissive to multiple viruses, and more effective in term of viral productivity. One critical characteristic of such cell lines is their ability to grow in suspension, in serum free conditions and at high cell densities. Influenza virus causing severe epidemics both in human and animals is an important threat to world healthcare. The repetitive apparition of influenza pandemic outbreaks in the last 20years explains that manufacturing sector is still looking for more effective production processes to replace/supplement embryonated egg-based process...
May 29, 2017: Vaccine
https://www.readbyqxmd.com/read/28569189/experimental-infections-of-mosquitoes-with-severe-fever-with-thrombocytopenia-syndrome-virus
#19
Shu-Yi Liang, Hong-Liang Chu, Xi-Ling Guo, Wei Wang, Hong-Na Chen, Yu-Fu Zhang, Yin Chen, Tao Wu, Chang-Jun Bao, Ming-Hao Zhou
BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is a newly identified emerging infectious disease, which is caused by a novel bunyavirus (termed SFTSV) in Asia. Although mosquitoes have not been identified as the primary vectors, as revealed by epidemiological surveys, their role in transmitting this SFTSV as a suspicious vector has not been validated. FINDINGS: In this study, we conducted experimental infections of mosquitoes with SFTSV to examine the role of mosquitoes in the transmission of the virus...
June 1, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28567016/streptomyces-sp-mum212-as-a-source-of-antioxidants-with-radical-scavenging-and-metal-chelating-properties
#20
Loh Teng-Hern Tan, Kok-Gan Chan, Tahir Mehmood Khan, Sarah Ibrahim Bukhari, Surasak Saokaew, Acharaporn Duangjai, Priyia Pusparajah, Learn-Han Lee, Bey-Hing Goh
Reactive oxygen species and other radicals potentially cause oxidative damage to proteins, lipids, and DNA which may ultimately lead to various complications including mutations, carcinogenesis, neurodegeneration, cardiovascular disease, aging, and inflammatory disease. Recent reports demonstrate that Streptomyces bacteria produce metabolites with potent antioxidant activity that may be developed into therapeutic drugs to combat oxidative stress. This study shows that Streptomyces sp. MUM212 which was isolated from mangrove soil in Kuala Selangor, Malaysia, could be a potential source of antioxidants...
2017: Frontiers in Pharmacology
keyword
keyword
102514
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"