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IS711 brucella

Mohamed Barkallah, Yaakoub Gharbi, Sonia Zormati, Nesrine Karkouch, Zouhir Mallek, Michel Gautier, Radhouane Gdoura, Imen Fendri
In this study, we conducted an investigation to determine the true prevalence of bovine and ovine brucellosis in central-eastern Tunisia. A total of 1134 veterinary samples taken from 130 ruminant herds were screened for brucellosis using IS711-based real-time PCR assay. Sera collected from the ruminants were tested using the Rose Bengal test and indirect enzyme-linked immunosorbent assay. Based on serological and molecular results, the true adjusted animal population level prevalence was 23.5 % in cattle, against 13...
October 1, 2016: Tropical Animal Health and Production
Akm Anisur Rahman, Dirk Berkvens, Claude Saegerman, David Fretin, Noor Muhammad, Akram Hossain, Emmanuel Abatih
INTRODUCTION: This study describes the seroprevalence of human brucellosis among pyretic patients and detection of Brucella abortus DNA from seropositive pyretic patients using real-time polymerase chain reaction (rtPCR) for the first time in Bangladesh. METHODOLOGY: Blood samples were collected from 300 pyretic patients from October 2007 to May 2008 and subjected to three serological tests: Rose-Bengal plate test (RBT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (iELISA)...
September 30, 2016: Journal of Infection in Developing Countries
Bhavna Sikarwar, Virendra V Singh, Pushpendra K Sharma, Ashu Kumar, Duraipandian Thavaselvam, Mannan Boopathi, Beer Singh, Yogesh K Jaiswal
Surface plasmon resonance (SPR) immunosensor using 4-mercaptobenzoic acid (4-MBA) modified gold (4-MBA/Au) SPR chip was developed first time for the detection of Brucella melitensis (B. melitensis) based on the screening of its complementary DNA target by using two different newly designed DNA probes of IS711 gene. Herein, interaction between DNA probes and target molecule are also investigated and result revealed that the interaction is spontaneous. The kinetics and thermodynamic results derived from the experimental data showed that the interaction between complementary DNA targets and probe 1 is more effective than that of probe 2...
September 18, 2016: Biosensors & Bioelectronics
Sharareh Mohammad Hasani, Reza Mirnejad, Jafar Amani, Mohamad Javad Vafadar
BACKGROUND: Rapid diagnosis and differentiation of Brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. This study aimed to identify and compare PCR-ELISA as a more accurate diagnositc test with other common molecular and serological tests. METHODS: In this experimental and sectional study, during March 2014 to Sep 2015, 52 blood samples of suspected patients with clinical symptoms of brucellosis were evaluated in medical centers all over Iran with serum titers higher than 1:80...
2016: Iranian Journal of Pathology
Anita Barua, Ashu Kumar, Duraipandian Thavaselvam, Smita Mangalgi, Archana Prakash, Sapana Tiwari, Sonia Arora, Kannusamy Sathyaseelan
BACKGROUND & OBJECTIVES: Brucellosis is endemic in the southern part of India. A combination of biochemical, serological and molecular methods is required for identification and biotyping of Brucella. The present study describes the isolation and biochemical, molecular characterization of Brucella melitensis from patients suspected for human brucellosis. METHODS: The blood samples were collected from febrile patients suspected to have brucellosis. A total of 18 isolates were obtained from 102 blood samples subjected to culture...
May 2016: Indian Journal of Medical Research
Ajay D Pathak, Z B Dubal, M Karunakaran, Swapnil P Doijad, Abhay V Raorane, R B Dhuri, M A Bale, Eaknath B Chakurkar, Dewanand R Kalorey, Nitin V Kurkure, Sukhadeo B Barbuddhe
Brucellosis is a highly contagious zoonotic infection affecting livestock and human beings. The disease has been reported worldwide except in few countries where it has been eradicated. The prevalence of brucellosis among cattle from 11 farms having a history of abortions was studied. A total of 481 samples comprising of blood, milk, vaginal swabs, vaginal discharges, placental tissues and fetal tissues were collected from 296 animals. Clinical samples were processed for the isolation of Brucella. Serum samples (n=296) were tested by Rose Bengal Plate Test (RBPT) and indirect ELISA...
August 2016: Comparative Immunology, Microbiology and Infectious Diseases
Shashank Purwar, Sharada C Metgud, Manohar B Mutnal, Mahantesh B Nagamoti, Chidanand S Patil
BACKGROUND: The culture has always been the gold standard test for diagnosis of human brucellosis but the conventional Brucella diagnostic tests viz. serology and culture are often beset with poor specificity & sensitivity respectively. The culture positivity rates for Brucella vary from 92% for bone marrow to 10% for non-blood samples and also dependent on the type of sample. The primary immune-determinant for Brucella species is the cell wall surface lipopolysaccharide, which is antigenically similar to other gram-negative rods...
February 2016: Journal of Clinical and Diagnostic Research: JCDR
Ana M Russo, Orlando A Mancebo, Carlos M Monzón, Juan J Gait, Rubén D Casco, Susana M Torioni de Echaide
An epidemiological study of brucellosis was carried out in 516 goats and mixed flocks (goat/sheep) from the three agro-ecological regions of Formosa province, Argentina. Serum samples from a total of 25401 goats and 2453 sheeps were analyzed using buffered plate agglutination test (BPAT) and complement fixation test (CFT). Bacteriological and PCR analyses on milk samples from goats in three flocks with a history of brucellosis and recent abortions were also performed. Brucellosis was detected in four of the nine departments of the province with an overall prevalence of 2% and an intra-flock prevalence ranging between 1% and 40%...
April 2016: Revista Argentina de Microbiología
Yue Wang, Hong Chen, Ying Liu, Jingzhu Zhou, Shijun Li, Yan Hang, Guangpeng Tang, Dingming Wang, Guichun Chen
OBJECTIVE: To identify and characterize the Brucella strains from Guizhou province in 2010-2013. METHODS: A total of 12 strains of Brucella suspicious bacteria were isolated in Guizhou province from 2010 to 2013. Four strains (GZLL3, GZLL4, GZLL11 and SH2) were isolated from goat blood samples and eight strains (SH4, GZZY, GZSQ, GZZA, BR13001, BR13004, BR13005 and BR13006) were isolated from blood samples of patient 12 Brucella suspicious strains were identified and characterized using conventional methods...
September 2015: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
J A Hammerl, R G Ulrich, C Imholt, H C Scholz, J Jacob, N Kratzmann, K Nöckler, S Al Dahouk
Brucellosis is a widespread zoonotic disease introduced from animal reservoirs to humans. In Germany, bovine and ovine/caprine brucellosis were eradicated more than a decade ago and mandatory measures in livestock have been implemented to keep the officially brucellosis-free status. In contrast, surveillance of wildlife is still challenging, and reliable data on the prevalence of brucellae in small mammal populations do not exist. To assess the epidemiology of Brucella spp. in rodents and shrews, a molecular survey was carried out...
September 23, 2015: Transboundary and Emerging Diseases
C Mathew, M Stokstad, T B Johansen, S Klevar, R H Mdegela, G Mwamengele, P Michel, L Escobar, D Fretin, J Godfroid
BACKGROUND: Brucellosis is a disease of worldwide public health and economic importance. Successful control is based on knowledge of epidemiology and strains present in an area. In developing countries, most investigations are based on serological assays. This study aimed at investigating a dairy herd experiencing abortions in order to establish within-herd seroprevalence to Brucella spp., identify, characterize Brucella strains by Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA-VNTR) and investigate possible spillover to other species...
2015: BMC Veterinary Research
Neha Dabral, Neeta Jain-Gupta, Mohamed N Seleem, Nammalwar Sriranganathan, Ramesh Vemulapalli
Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in mammals. Brucella strains containing the O-polysaccharide in their cell wall structure exhibit a smooth phenotype whereas the strains devoid of the polysaccharide show rough phenotype. B. abortus strain RB51 is a stable rough attenuated mutant which is used as a licensed live vaccine for bovine brucellosis. Previous studies have shown that the wboA gene, which encodes a glycosyltransferase required for the synthesis of O-polysaccharide, is disrupted in B...
2015: Frontiers in Cellular and Infection Microbiology
Shahzad Ali, Shamim Akhter, Heinrich Neubauer, Falk Melzer, Iahtasham Khan, Qurban Ali, Muhammad Irfan
INTRODUCTION: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan. METHODOLOGY: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT)...
May 2015: Journal of Infection in Developing Countries
Richar Ivan Rodríguez-Hidalgo, Javier Contreras-Zamora, Washington Benitez Ortiz, Karina Guerrero-Viracocha, Holger Salcan-Guaman, Elizabeth Minda, Lenin Ron Garrido
The Province of Santo Domingo de los Tsáchilas in Ecuador represents the largest informal cattle market. Because of its strategic position, cattle movement is very high and therefore we selected this region, to determine the strain variation of Brucella sp. Part of the study aimed at the isolation, biotyping, and genotyping of Brucella species from milk and supra-mammary lymph nodes of sero-positive bovines, using selective Farrell medium, biochemical assays, and IS711-PCR, AMOS-PCR, and HOOF-Prints techniques...
2015: Frontiers in Public Health
Gamal Wareth, Falk Melzer, Mandy C Elschner, Heinrich Neubauer, Uwe Roesler
INTRODUCTION: Brucellosis in Egypt is an endemic disease among animals and humans. In endemic developing countries, dairy products produced from untreated milk are a potential threat to public health. The aim of this study was to detect brucellae in milk and milk products produced from apparently healthy animals to estimate the prevalence of contamination. METHODOLOGY: Two hundred and fifteen unpasteurized milk samples were collected from apparently healthy cattle (n = 72) and buffaloes (n = 128) reared on small farms, and from milk shops (n = 15) producing dairy products for human consumption...
October 2014: Journal of Infection in Developing Countries
Maryam Garshasbi, Ali Ramazani, Rahim Sorouri, Siamak Javani, Soheila Moradi
Brucella is an intracellular pathogen capable of infecting animals and humans. The aim of this study was to identify Brucella spp in sera of high risk individuals by a polymerase chain reaction (PCR)-based method. A total of 180 patients suspected to have Brucellosis were examined by serological tests. To establish a PCR protocol for diagnosis of active brucellosis, DNA was extracted from the serum samples by using a commercial kit. PCR amplification was done for detection of Brocella DNA using BCSP31 target gene and IS711 locus...
2014: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Adrian M Whatmore, Nicholas Davison, Axel Cloeckaert, Sascha Al Dahouk, Michel S Zygmunt, Simon D Brew, Lorraine L Perrett, Mark S Koylass, Gilles Vergnaud, Christine Quance, Holger C Scholz, Edward J Dick, Gene Hubbard, Natalia E Schlabritz-Loutsevitch
Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60(T) and F8/08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus Brucella. This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate...
December 2014: International Journal of Systematic and Evolutionary Microbiology
Elisa Maio, Lineke Begeman, Yvette Bisselink, Peter van Tulden, Lidewij Wiersma, Sjoukje Hiemstra, Robin Ruuls, Andrea Gröne, Hendrik-Ido-Jan Roest, Peter Willemsen, Joke van der Giessen
The presence of Brucella (B.) spp. in harbour porpoises stranded between 2008 and 2011 along the Dutch coast was studied. A selection of 265 tissue samples from 112 animals was analysed using conventional and molecular methods. In total, 4.5% (5/112) of the animals corresponding with 2.3% (6/265) Brucella positive tissue samples were Brucella positive by culture and these were all confirmed by real-time polymerase chain reaction (real-time PCR) based on the insertion element 711 (IS711). In addition, two more Brucella-positive tissue samples from two animals collected in 2011 were identified using real-time PCR resulting in an overall Brucella prevalence of 6...
September 17, 2014: Veterinary Microbiology
Diana Martínez, Carolina Thompson, Graciela Draghi, Vilma Canavesio, Roberto Jacobo, Patricia Zimmer, Sebastián Elena, Ana M Nicola, Susana Torioni de Echaide
An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B...
September 17, 2014: Veterinary Microbiology
Gemma L Kay, Martin J Sergeant, Valentina Giuffra, Pasquale Bandiera, Marco Milanese, Barbara Bramanti, Raffaella Bianucci, Mark J Pallen
Shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from contemporary and historical material. We sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century middle-aged male excavated from the medieval Sardinian settlement of Geridu. We obtained 6.5-fold coverage of a Brucella melitensis genome. Sequence reads from this genome showed signatures typical of ancient or aged DNA. Despite the relatively low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome within a clade of B...
2014: MBio
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