keyword
MENU ▼
Read by QxMD icon Read
search

manipulate

keyword
https://www.readbyqxmd.com/read/29777738/establishment-of-an-efficient-genetic-transformation-method-in-dunaliella-tertiolecta-mediated-by-agrobacterium-tumefaciens
#1
Claudia D Norzagaray-Valenzuela, Lourdes J Germán-Báez, Marco A Valdez-Flores, Sergio Hernández-Verdugo, Luke M Shelton, Angel Valdez-Ortiz
Microalgae are photosynthetic microorganisms widely used for the production of highly valued compounds, and recently they have been shown to be promising as a system for the heterologous expression of proteins. Several transformation methods have been successfully developed, from which the Agrobacterium tumefaciens-mediated method remains the most promising. However, microalgae transformation efficiency by A. tumefaciens is shown to vary depending on several transformation conditions. The present study aimed to establish an efficient genetic transformation system in the green microalgae Dunaliella tertiolecta using the A...
May 16, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29777713/development-and-psychometric-validation-of-capacity-assessment-of-prosthetic-performance-for-the-upper-limb-cappful
#2
Nathan T Kearns, Jennifer K Peterson, Lisa Smurr Walters, Warren T Jackson, John M Miguelez, Tiffany Ryan
OBJECTIVES: (1) Develop a performance-based measure for adult upper limb (UL) prosthetic functioning through broad (i.e. overall performance) and functional domain-specific (e.g., control skills) assessment of commonplace activities; (2) conduct initial psychometric evaluation of the Capacity Assessment of Prosthetic Performance for the Upper Limb (CAPPFUL). DESIGN: Internal consistency of CAPPFUL and interrater reliability for task, functional domain, and full scale (sub)scores among three independent raters were estimated...
May 16, 2018: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/29777709/amelioration-of-myocardial-ischemia-reperfusion-injury-by-sirt4-involves-mitochondrial-protection-and-reduced-apoptosis
#3
Guangwei Zeng, Hui Liu, Haiyan Wang
Apoptosis and mitochondria dysfunction are key contributors to myocardial ischemia-reperfusion (MI-R) injury. SIRT4, a mitochondrial-localized sirtuin, controls cellular energy metabolism and stress response, and is abundantly present in the heart, however, its role in MI-R injury is not clear. In the current study, we demonstrate that SIRT4 is downregulated in cardiomyocytes both in vitro and in vivo models after MI-R. Functionally, SIRT4 overexpression decreases myocardial infarct size and serum creatine phosphokinase (CPK) level, and vice versa, SIRT4 depletion by siRNA increases myocardial infarct size and serum CPK level...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777679/development-of-dnazyme-based-pcr-signal-cascade-amplification-for-visual-detection-of-listeria-monocytogenes-in-food
#4
Zhanmin Liu, Chenhui Yao, Cuiyun Yang, Yanming Wang, Sibao Wan, Junyi Huang
Listeria monocytogenes is an important food-borne pathogen, and it can cause severe diseases. Rapid detection of L. monocytogenes is crucial to control this pathogen. A simple and robust strategy based on the cascade of PCR and G-quadruplex DNAzyme catalyzed reaction was used to detect L. monocytogenes. In the presence of hemin and the aptamer formed during PCR, the catalytic horseradish peroxidase-mimicking G-quadruplex DNAzymes allow the colorimetric responses of target DNA from L. monocytogenes. This assay can detect genomic DNA of L...
May 16, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29777676/cognitive-effects-of-rhythmic-auditory-stimulation-in-parkinson-s-disease-a-p300-study
#5
Juan Lei, Nadine Conradi, Cornelius Abel, Stefan Frisch, Alla Brodski-Guerniero, Marcel Hildner, Christian A Kell, Jochen Kaiser, Maren Schmidt-Kassow
Rhythmic auditory stimulation (RAS) may compensate dysfunctions of the basal ganglia (BG), involved with intrinsic evaluation of temporal intervals and action initiation or continuation. In the cognitive domain, RAS containing periodically presented tones facilitates young healthy participants' attention allocation to anticipated time points, indicated by better performance and larger P300 amplitudes to periodic compared to random stimuli. Additionally, active auditory-motor synchronization (AMS) leads to a more precise temporal encoding of stimuli via embodied timing encoding than stimulus presentation adapted to the participants' actual movements...
May 16, 2018: Brain Research
https://www.readbyqxmd.com/read/29777591/bioengineering-triacetic-acid-lactone-production-in-yarrowia-lipolytica-for-pogostone-synthesis
#6
James Yu, Jenny Landberg, Farbod Shavarebi, Virginia Bilanchone, Adam Okerlund, Umayangani Wanninayake, Le Zhao, George Kraus, Suzanne Sandmeyer
Yarrowia lipolytica is an oleaginous yeast that is recognized for its ability to accumulate high levels of lipids, which can serve as precursors to biobased fuels and chemicals. Polyketides such as triacetic acid lactone (TAL) can also serve as a precursor for diverse commodity chemicals. This study used Y. lipolytica as a host organism for the production of TAL via expression of the 2-pyrone synthase gene from Gerbera hybrida. Induction of lipid biosynthesis by nitrogen-limited growth conditions increased TAL titers...
May 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29777473/interoceptive-accuracy-in-youth-with-tic-disorders-exploring-links-with-premonitory-urge-anxiety-and-quality-of-life
#7
Victoria Pile, Jennifer Y F Lau, Marta Topor, Tammy Hedderly, Sally Robinson
Aberrant interoceptive accuracy could contribute to the co-occurrence of anxiety and premonitory urge in chronic tic disorders (CTD). If it can be manipulated through intervention, it would offer a transdiagnostic treatment target for tics and anxiety. Interoceptive accuracy was first assessed consistent with previous protocols and then re-assessed following an instruction attempting to experimentally enhance awareness. The CTD group demonstrated lower interoceptive accuracy than controls but, importantly, this group difference was no longer significant following instruction...
May 18, 2018: Journal of Autism and Developmental Disorders
https://www.readbyqxmd.com/read/29777438/no-evidence-of-binding-items-to-spatial-configuration-representations-in-visual-working-memory
#8
Rob Udale, Simon Farrell, Christopher Kent
When detecting changes in visual features (e.g., colour or shape), object locations, represented as points within a configuration, might also be automatically represented in working memory. If the configuration of a scene is represented automatically, the locations of individual items might form part of this representation, irrespective of their relevance to the task. Participants took part in a change-detection task in which they studied displays containing different sets of items (shapes, letters, objects), which varied in their task relevance...
May 17, 2018: Memory & Cognition
https://www.readbyqxmd.com/read/29777186/anti-reflectance-investigation-of-a-micro-nano-hybrid-structure-fabricated-by-dry-wet-etching-methods
#9
Xiao Tan, Zhi Tao, Mingxing Yu, Hanxiao Wu, Haiwang Li
Black silicon fabrication and manipulation have been well reported by institutes around the world and are quite useful for solar absorption and photovoltaic conversion. In this study, silicon micro-nano hybrid structures were fabricated, and the morphologies of the hybrid structures were analyzed. This paper studied nanostructures formed on tips, pits and a flat surface using a dry etching method and a wet etching method. In terms of nanostructure morphology, nanostructures etched by the wet etching method (13 μm) were taller than those etched by the dry etching method (1 μm), but the wet etched morphology was less organized...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29776820/mechanical-interaction-between-neighboring-muscles-in-human-upper-limb-evidence-for-epimuscular-myofascial-force-transmission-in-humans
#10
Yasuhide Yoshitake, Daiki Uchida, Kosuke Hirata, Dean L Mayfield, Hiroaki Kanehisa
To confirm the existence of epimuscular myofascial force transmission in humans, this study examined if manipulating joint angle to stretch the muscle can alter the shear modulus of a resting adjacent muscle, and whether there are regional differences in this response. The biceps brachii (BB: manipulated muscle) and the brachialis (BRA: resting adjacent muscle) were deemed suitable for this study because they are neighboring, yet have independent tendons that insert onto different bones. In order to manipulate the muscle length of BB only, the forearm was passively set at supination, neutral, and pronation positions...
May 3, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29776613/high-resolution-melting-analysis-hrm-for-the-assessment-of-clonality-in-feline-b-cell-lymphomas
#11
Manfred Henrich, Svenja Scheffold, Werner Hecht, Manfred Reinacher
Analysis of clonality is gaining importance in diagnosing lymphomas in veterinary medicine. Usually, PCR for the analysis of antigen receptor rearrangement (PARR) is followed by electrophoretic separation of the PCR products. Aim of this study was to test the feasibility of HRM for the assessment of clonality in B-cell lymphomas of cats. High resolution melting analysis differentiates PCR products by their different melting point using the decrease in fluorescence of an intercalating dye during melting of the PCR product...
June 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29776169/effects-of-nucleosome-stability-on-remodeler-catalyzed-repositioning
#12
Aaron M Morgan, Sarah E LeGresley, Koan Briggs, Gada Al-Ani, Christopher J Fischer
Chromatin remodelers are molecular motors that play essential roles in the regulation of nucleosome positioning and chromatin accessibility. These machines couple the energy obtained from the binding and hydrolysis of ATP to the mechanical work of manipulating chromatin structure through processes that are not completely understood. Here we present a quantitative analysis of nucleosome repositioning by the imitation switch (ISWI) chromatin remodeler and demonstrate that nucleosome stability significantly impacts the observed activity...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29776123/efficient-thermal-diode-with-ballistic-spacer
#13
Shunda Chen, Davide Donadio, Giuliano Benenti, Giulio Casati
Thermal rectification is of importance not only for fundamental physics, but also for potential applications in thermal manipulations and thermal management. However, thermal rectification effect usually decays rapidly with system size. Here, we show that a mass-graded system, with two diffusive leads separated by a ballistic spacer, can exhibit large thermal rectification effect, with the rectification factor independent of system size. The underlying mechanism is explained in terms of the effective size-independent thermal gradient and the match or mismatch of the phonon bands...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29776116/depletion-zones-and-crystallography-on-pinched-spheres
#14
Jingyuan Chen, Xiangjun Xing, Zhenwei Yao
Understanding the interplay between ordered structures and substrate curvature is an interesting problem with versatile applications, including functionalization of charged supramolecular surfaces and modern microfluidic technologies. In this work, we investigate the two-dimensional packing structures of charged particles confined on a pinched sphere. By continuously pinching the sphere, we observe cleavage of elongated scars into pleats, proliferation of disclinations, and subsequently, emergence of a depletion zone at the negatively curved waist that is completely void of particles...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29776072/revised-model-for-the-radiation-force-exerted-by-standing-surface-acoustic-waves-on-a-rigid-cylinder
#15
Shen Liang, Wang Chaohui
In this paper, a model for the radiation force exerted by standing surface acoustic waves (SSAWs) on a rigid cylinder in inviscid fluids is extended to account for the dependence on the Rayleigh angle. The conventional model for the radiation force used in the SSAW-based applications is developed in plane standing waves, which fails to predict the movement of the cylinder in the SSAW. Our revised model reveals that, in the direction normal to the piezoelectric substrate on which the SSAW is generated, acoustic radiation force can be large enough to drive the cylinder even in the long-wavelength limit...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29775938/treatment-of-perfluoroalkyl-acids-by-heat-activated-persulfate-under-conditions-representative-of-in-situ-chemical-oxidation
#16
Thomas A Bruton, David L Sedlak
Perfluoroalkyl acids (PFAAs) are a class of organic contaminants notable for their extreme persistence. The unique chemical properties of these compounds make them difficult to remove from water using most standard water treatment techniques. To gain insight into the possibility of remediating contaminated groundwater by in situ chemical oxidation with heat-activated persulfate, PFAA removal and the generation of transformation products were evaluated under laboratory conditions. Solution pH had a strong influence on the removal of perfluorooctanoic acid (PFOA), resulting in its transformation into shorter-chain perfluorocarboxylic acids (PFCAs) at pH values below 3...
April 21, 2018: Chemosphere
https://www.readbyqxmd.com/read/29775837/predator-based-psychosocial-stress-model-of-ptsd-differentially-influences-voluntary-ethanol-consumption-depending-on-methodology
#17
Phillip R Zoladz, Eric D Eisenmann, Robert M Rose, Brooke A Kohls, Brandon L Johnson, Kiera L Robinson, Megan E Heikkila, Kasey E Mucher, Madelaine R Huntley
Post-traumatic stress disorder (PTSD) is a debilitating psychological disorder typified by diagnostic symptom clusters including hyperarousal, avoidance, negative cognitions and mood, and intrusive re-experiencing of the traumatic event. Patients with PTSD have been reported to self-medicate with alcohol to ameliorate hyperarousal symptoms associated with the disorder. Research utilizing rodent models of PTSD to emulate this behavioral phenomenon has thus far yielded inconsistent results. In the present study, we examined the effects of a predator-based psychosocial stress model of PTSD on voluntary ethanol consumption...
January 8, 2018: Alcohol
https://www.readbyqxmd.com/read/29775823/plasticity-of-population-coding-in-primary-sensory-cortex
#18
REVIEW
Amy M LeMessurier, Daniel E Feldman
That experience shapes sensory tuning in primary sensory cortex is well understood. But effective neural population codes depend on more than just sensory tuning. Recent population imaging and recording studies have characterized population codes in sensory cortex, and tracked how they change with sensory manipulations and training on perceptual learning tasks. These studies confirm sensory tuning changes, but also reveal other features of plasticity, including sensory gain modulation, restructuring of firing correlations, and differential routing of information to output pathways...
May 15, 2018: Current Opinion in Neurobiology
https://www.readbyqxmd.com/read/29775816/understanding-the-role-of-hydrogen-bonding-in-the-aggregation-of-fumed-silica-particles-in-triglyceride-solvents
#19
Catherine P Whitby, Melinda Krebsz, Samuel J Booty
HYPOTHESIS: Fumed silica particles are thought to thicken organic solvents into gels by aggregating to form networks. Hydrogen bonding between silanol groups on different particle surfaces causes the aggregation. The gel structure and hence flow behaviour is altered by varying the proportion of silanol groups on the particle surfaces. However, characterising the gel using rheology measurements alone is not sufficient to optimise the aggregation. We have used confocal microscopy to characterise the changes in the network microstructure caused by altering the particle surface chemistry...
May 12, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29775662/multivoxel-pattern-similarity-suggests-the-integration-of-temporal-duration-in-hippocampal-event-sequence-representations
#20
Sathesan Thavabalasingam, Edward B O'Neil, Andy C H Lee
Recent rodent work suggests the hippocampus may provide a temporal representation of event sequences, in which the order of events and the interval durations between them are encoded. There is, however, limited human evidence for the latter, in particular whether the hippocampus processes duration information pertaining to the passage of time rather than qualitative or quantitative changes in event content. We scanned participants while they made match/mismatch judgements on each trial between a study sequence of events and a subsequent test sequence...
May 15, 2018: NeuroImage
keyword
keyword
102510
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"