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https://www.readbyqxmd.com/read/28445849/a-reference-measurement-procedure-for-amino-acids-in-blood-using-isotope-dilution-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#1
Juok Kim, Tran Thi Thanh Huong, Seon-Pyo Hong, Ji-Seon Jeong
We described a reference measurement procedure for amino acid (AA) quantification in blood samples based on deproteinization with 5-sulfosalicylic acid (SSA) and an isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry (LC-MS) method. The serum was deproteinized with 15% v/v SSA and the supernatant was injected directly into the LC-MS system without further processing. Compared with the use of other precipitants and water as a control, five model AAs-valine, isoleucine, leucine, tyrosine, and phenylalanine-in the SSA-treated samples showed ionization enhancement as well as stable background signals without significant ion suppression effects...
April 17, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28445753/pyroglutamate-modified-amyloid-%C3%AE-3-42-shows-%C3%AE-helical-intermediates-before-amyloid-formation
#2
Christina Dammers, Kerstin Reiss, Lothar Gremer, Justin Lecher, Tamar Ziehm, Matthias Stoldt, Melanie Schwarten, Dieter Willbold
Pyroglutamate-modified amyloid-β (pEAβ) has been described as a relevant Aβ species in Alzheimer's-disease-affected brains, with pEAβ (3-42) as a dominant isoform. Aβ (1-40) and Aβ (1-42) have been well characterized under various solution conditions, including aqueous solutions containing trifluoroethanol (TFE). To characterize structural properties of pEAβ (3-42) possibly underlying its drastically increased aggregation propensity compared to Aβ (1-42), we started our studies in various TFE-water mixtures and found striking differences between the two Aβ species...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28445637/control-of-charge-carriers-trapping-and-relaxation-in-hematite-by-oxygen-vacancy-charge-ab-initio-non-adiabatic-molecular-dynamics
#3
Zhaohui Zhou, Jin Liu, Run Long, Linqiu Li, Liejin Guo, Oleg V Prezhdo
Ultrafast charge recombination in hematite (α-Fe2O3) severely limits its applications in solar energy conversion and utilization, for instance, in photo-electrochemical water splitting. We report the first time-domain ab initio study of charge relaxation dynamics in α-Fe2O3 with and without the oxygen vacancy (Ov) defect, using non-adiabatic molecular dynamics implemented within time-dependent density functional theory. The simulations show that the hole trapping is the rate-limiting step in the electron-hole recombination process for both neutral and ionized Ov systems...
April 26, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28445635/synthesis-and-enhanced-corrosion-protection-performance-of-reduced-graphene-oxide-nanosheet-znal-layered-double-hydroxides-composite-film-by-hydrothermal-continuous-flow-method
#4
Xiaohu Luo, Song Yuan, Xinyu Pan, Caixia Zhang, Shuo Du, Yali Liu
Effective prevention of the water, oxygen, and chloride ions held in the hydrotalcite interlayer from diffusing through the layered double hydroxides ( LDH ) layer is crucial importance for its further practical application in the corrosion protection. In this study, a hybrid reduced graphene oxide ( RGO ) nanosheet /Zn2+/Al3+ layered double hydroxides ( RGO/ZnAl-LDH ) composite film with an excellent corrosion protection property on the 6N01 aluminum alloy surface was synthesized by a newly and facile hydrothermal continuous flow method,which can enable direct growth on the substrate...
April 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28445408/algal-proteins-extraction-application-and-challenges-concerning-production
#5
REVIEW
Stephen Bleakley, Maria Hayes
Population growth combined with increasingly limited resources of arable land and fresh water has resulted in a need for alternative protein sources. Macroalgae (seaweed) and microalgae are examples of under-exploited "crops". Algae do not compete with traditional food crops for space and resources. This review details the characteristics of commonly consumed algae, as well as their potential for use as a protein source based on their protein quality, amino acid composition, and digestibility. Protein extraction methods applied to algae to date, including enzymatic hydrolysis, physical processes, and chemical extraction and novel methods such as ultrasound-assisted extraction, pulsed electric field, and microwave-assisted extraction are discussed...
April 26, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28445394/spectroscopic-detection-of-glyphosate-in-water-assisted-by-laser-ablated-silver-nanoparticles
#6
Rafael Eleodoro De Góes, Marcia Muller, José Luís Fabris
Glyphosate is one of the most widely used herbicides in the world. Its safety for both human health and aquatic biomes is a subject of wide debate. There are limits to glyphosate's presence in bodies of water, and it is usually detected through complex analytical procedures. In this work, the presence of glyphosate is detected directly through optical interrogation of aqueous solution. For this purpose, silver nanoparticles were produced by pulsed laser ablation in liquids. Limits of detection of 0.9 mg/L and 3...
April 26, 2017: Sensors
https://www.readbyqxmd.com/read/28445078/effects-of-high-intensity-interval-training-on-canoeing-performance
#7
Ming-Ta Yang, Mien-Mien Lee, Shu-Ching Hsu, Kuei-Hui Chan
The purpose of this study was to compare the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) utilizing a canoeing ergometer on endurance determinants, as well as aerobic and anaerobic performances among flat-water canoeists. Fourteen well-trained male flat-water canoeists were divided into an HIIT group or an MICT group. All subjects performed a continuous graded exercise test (GXT) and three fixed-distance (200, 500, and 1000 m) performance tests on a canoeing ergometer to determine canoeing economy, peak oxygen uptake (VO2peak), and power at VO2peak, and to calculate the critical velocity (CV) and anaerobic work capacity before and after the training programmes...
April 26, 2017: European Journal of Sport Science
https://www.readbyqxmd.com/read/28445042/structural-transformation-of-mno2-during-the-oxidation-of-bisphenol-a
#8
Sarah Balgooyen, Peter J Alaimo, Christina K Remucal, Matthew Ginder-Vogel
Bisphenol A (BPA) is an endocrine-disrupting compound widely used in the plastic industry and found in natural waters at concentrations considered harmful for aquatic life. BPA is susceptible to oxidation by Mn(III/IV) oxides, which are commonly found in near-surface environments. Here, we quantify BPA oxidation rates and the formation of its predominant product, 4-hydroxycumyl alcohol (HCA), in tandem with transformation of a synthetic, Mn(III)-rich δ-MnO2. To investigate the effect of Mn oxide structural changes on BPA oxidation rate, twelve sequential additions of 80 μM BPA are performed at pH 7...
April 26, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28444620/environmental-pollution-with-antimicrobial-agents-from-bulk-drug-manufacturing-industries-in-hyderabad-south-india-is-associated-with-dissemination-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-pathogens
#9
Christoph Lübbert, Christian Baars, Anil Dayakar, Norman Lippmann, Arne C Rodloff, Martina Kinzig, Fritz Sörgel
PURPOSE: High antibiotic and antifungal concentrations in wastewater from anti-infective drug production may exert selection pressure for multidrug-resistant (MDR) pathogens. We investigated the environmental presence of active pharmaceutical ingredients and their association with MDR Gram-negative bacteria in Hyderabad, South India, a major production area for the global bulk drug market. METHODS: From Nov 19 to 28, 2016, water samples were collected from the direct environment of bulk drug manufacturing facilities, the vicinity of two sewage treatment plants, the Musi River, and habitats in Hyderabad and nearby villages...
April 26, 2017: Infection
https://www.readbyqxmd.com/read/28443667/identification-of-a-phosphodiesterase-inhibiting-fraction-from-roasted-coffee-coffea-arabica-through-activity-guided-fractionation
#10
Teresa Röhrig, David Liesenfeld, Elke Richling
Recent reports that coffee can significantly inhibit cAMP phosphodiesterases (PDE) in vitro, as well as in vivo, have added another beneficial effect of coffee consumption. However, the PDE-inhibiting substances remain mostly unknown. We chose activity-guided fractionation and an in vitro test system to identify the coffee components responsible for PDE inhibition. This approach indicated that a fraction of melanoidins reveals strong PDE inhibiting potential (IC50 = 130 ± 42 µg/mL). These melanoidins were characterized as water soluble, low molecular weight melanoidins (< 3kDa) with a nitrogen content of 4...
April 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28443626/nanodiamond-enhanced-mri-via-in-situ-hyperpolarization
#11
David E J Waddington, Mathieu Sarracanie, Huiliang Zhang, Najat Salameh, David R Glenn, Ewa Rej, Torsten Gaebel, Thomas Boele, Ronald L Walsworth, David J Reilly, Matthew S Rosen
Nanodiamonds are of interest as nontoxic substrates for targeted drug delivery and as highly biostable fluorescent markers for cellular tracking. Beyond optical techniques, however, options for noninvasive imaging of nanodiamonds in vivo are severely limited. Here, we demonstrate that the Overhauser effect, a proton-electron polarization transfer technique, can enable high-contrast magnetic resonance imaging (MRI) of nanodiamonds in water at room temperature and ultra-low magnetic field. The technique transfers spin polarization from paramagnetic impurities at nanodiamond surfaces to (1)H spins in the surrounding water solution, creating MRI contrast on-demand...
April 26, 2017: Nature Communications
https://www.readbyqxmd.com/read/28443524/risk-factors-for-prelacteal-feeding-in-sub-saharan-africa-a-multilevel-analysis-of-population-data-from-twenty-two-countries
#12
Anselm S Berde, Hilal Ozcebe
OBJECTIVE: To examine the risk factors of prelacteal feeding (PLF) among mothers in sub-Saharan Africa (SSA). DESIGN: We pooled data from Demographic and Health Surveys in twenty-two SSA countries. The key outcome variable was PLF. A multilevel logistic regression model was used to explore factors associated with PLF. SETTING: Demographic and Health Surveys in twenty-two SSA countries. SUBJECTS: Mother-baby pairs (n 95348)...
April 26, 2017: Public Health Nutrition
https://www.readbyqxmd.com/read/28443331/coupling-between-the-dynamics-of-water-and-surfactants-in-lyotropic-liquid-crystals
#13
Jesse Gatten McDaniel, Arun Yethiraj
Bilayers composed of lipid or surfactant molecules are central to biological membranes and lamellar lyotropic liquid crystalline (LLC) phases. Common to these systems are phases that exhibit either ordered or disordered packing of the hydrophobic tails. In this work, we study the impact of surfactant ordering, i.e., disordered Lα and ordered Lβ LLC phases, on the dynamics of water in the lamellar phases of dicarboxylate gemini surfactants. We study the different phases at identical hydration levels by changing the length of the hydrophobic tails; surfactants with shorter tails form Lα phases and those with longer tails form Lβ phases...
April 26, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28443322/crassulacean-acid-metabolism-as-a-continuous-trait-variability-in-the-contribution-of-crassulacean-acid-metabolism-cam-in-populations-of-portulacaria-afra
#14
Lonnie J Guralnick, Kate Gladsky
Portulacaria afra L. is a dominant facultative CAM species growing in the Southeastern Cape of South Africa. P. afra is well adapted to regions of the Spekboom thicket in areas of limited and sporadic rainfall. P. afra populations occur in isolated drainages. We hypothesized the utilization of CAM would vary in the different populations in response to rainfall and temperature gradients. Carbon isotope composition can be used to determine the contribution of CAM in leaf tissue. P. afra leaves of populations were analyzed in transects running south to north and east to west in locations from the coast to elevations of 1400 m...
April 2017: Heliyon
https://www.readbyqxmd.com/read/28443116/comparative-analysis-of-phosphoproteome-remodeling-after-short-term-water-stress-and-aba-treatments-versus-longer-term-water-stress-acclimation
#15
Govinal B Bhaskara, Thao T Nguyen, Tsu-Hao Yang, Paul E Verslues
Several studies have used short term dehydration, osmotic stress or Abscisic Acid (ABA) treatments to identify the initial protein phosphorylation-dephosphorylation responses to drought and low water potential or ABA treatments. However, longer term drought acclimation leads to altered expression of many kinases and phosphatases suggesting that it may also produce unique changes in phosphoproteome composition. To get a better overview of the state of drought-related phosphoproteomics and investigate this question of short versus longer term phosphoproteome regulation, we compared three Arabidopsis thaliana studies analyzing short term phosphoproteome changes to recent data from our laboratory analyzing phosphoproteome changes after a longer drought acclimation treatment...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28442768/synergistic-effect-of-nitrate-doped-tio2-aerosols-on-the-fast-photochemical-oxidation-of-formaldehyde
#16
Jing Shang, Wei Wei Xu, Chun Xiang Ye, Christian George, Tong Zhu
The uptake of formaldehyde (HCHO) on mineral dust affects its budget as well as particle properties, yet the process has not yet been fully investigate. Here, TiO2 and nitrate-doped TiO2 aerosols were used as proxies for mineral dust, and the uptake of HCHO was explored in a chamber under both dark and illuminated conditions. The uptake loss of HCHO on UV-illuminated aerosols is 2-9 times faster than its gaseous photolysis in our experimental system. The uptake coefficient in the range of 0.43-1.68 × 10(-7) is 1-2 orders of magnitude higher than previous reports on model mineral dust particles...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28441957/landscape-structure-affects-distribution-of-potential-disease-vectors-diptera-culicidae
#17
Carina Zittra, Simon Vitecek, Adelheid G Obwaller, Heidemarie Rossiter, Barbara Eigner, Thomas Zechmeister, Johann Waringer, Hans-Peter Fuehrer
BACKGROUND: Vector-pathogen dynamics are controlled by fluctuations of potential vector communities, such as the Culicidae. Assessment of mosquito community diversity and, in particular, identification of environmental parameters shaping these communities is therefore of key importance for the design of adequate surveillance approaches. In this study, we assess effects of climatic parameters and habitat structure on mosquito communities in eastern Austria to deliver these highly relevant baseline data...
April 26, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28441739/the-use-of-immus-in-a-water-environment-instrument-validation-and-application-of-3d-multi-body-kinematic-analysis-in-medicine-and-sport
#18
Anna Lisa Mangia, Matteo Cortesi, Silvia Fantozzi, Andrea Giovanardi, Davide Borra, Giorgio Gatta
The aims of the present study were the instrumental validation of inertial-magnetic measurements units (IMMUs) in water, and the description of their use in clinical and sports aquatic applications applying customized 3D multi-body models. Firstly, several tests were performed to map the magnetic field in the swimming pool and to identify the best volume for experimental test acquisition with a mean dynamic orientation error lower than 5°. Successively, the gait and the swimming analyses were explored in terms of spatiotemporal and joint kinematics variables...
April 22, 2017: Sensors
https://www.readbyqxmd.com/read/28441602/fluoride-and-iodine-enrichment-in-groundwater-of-north-china-plain-evidences-from-speciation-analysis-and-geochemical-modeling
#19
Junxia Li, Hailing Zhou, Kun Qian, Xianjun Xie, Xiaobin Xue, Yijun Yang, Yanxin Wang
To better understand the enrichment of fluoride and iodine in groundwater at North China Plain (NCP), speciation analysis and geochemical modeling were conducted to identify the key hydrochemical processes controlling their mobilization in groundwater system. Groundwater fluoride and iodine concentrations ranged from 0.18 to 5.59mg/L and from 1.51 to 1106μg/L, respectively, and approximately 63% and 32.3% of groundwater fluoride and iodine were higher than the guidelines for drinking water (1.5mg/L and 150μg/L)...
April 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28441576/fate-and-redistribution-of-perfluoroalkyl-acids-through-afff-impacted-groundwater
#20
Jennifer Bräunig, Christine Baduel, Amy Heffernan, Anna Rotander, Eric Donaldson, Jochen F Mueller
Leaching of perfluoroalkyl acids (PFAAs) from a local point source, a fire-fighting training area, has led to extensive contamination of a groundwater aquifer which has spread underneath part of a nearby town, Oakey, situated in the State of Queensland, Australia. Groundwater is extracted by residents from privately owned wells for daily activities such as watering livestock and garden beds. The concentration of 10 PFAAs in environmental and biological samples (water, soil, grass, chicken egg yolk, serum of horses, cattle and sheep), as well as human serum was investigated to determine the extent of contamination in the town and discuss fate and redistribution of PFAAs...
April 22, 2017: Science of the Total Environment
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