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Yushi Uetera, Hiroshi Yasuhara, Naohito Kumada, Yoshiki Misawa, Yoshiyuki Terada, Yoshio Satou, Tamaki Suhara, Kunio Kawamura
Recently, the use of filters has come into light for sanitizing water plants. This study investigated the role of heat-tolerant ultrafilters (UFs) for the remediation of reverse osmosis (RO) plants using periodic thermal disinfection. Two completely identical RO plants (RO plants A & B) were installed in 2006 for surgical hand antisepsis in the operating theater. RO water was stored in the 300-liter storage tank and re-circulated in the 190 meter-long loop delivering water to 12 faucets in each RO plant...
March 15, 2018: PDA Journal of Pharmaceutical Science and Technology
Mengyuan Xu, Jing Yan, Yingsan Geng, Kun Zhang, Chao Sun
An innovative array of magnetic coils (the discrete Rogowski coil-RC) with the advantages of flexible structure, miniaturization and mass producibility is investigated. First, the mutual inductance between the discrete RC and circular and rectangular conductors are calculated using the magnetic vector potential (MVP) method. The results are found to be consistent with those calculated using the finite element method, but the MVP method is simpler and more practical. Then, the influence of conductor section parameters, inclination, and eccentricity on the accuracy of the discrete RC is calculated to provide a reference...
March 13, 2018: Sensors
Marco Mangiagalli, Guy Sarusi, Aleksei Kaleda, Maya Bar Dolev, Valentina Nardone, Vittoria Federica Vena, Ido Braslavsky, Marina Lotti, Marco Nardini
Ice Binding Proteins (IBPs) contribute to the survival of many living beings at sub-zero temperature by controlling the formation and growth of ice crystals. This work investigates the structural basis of the ice binding properties of EfcIBP, obtained from Antarctic bacteria. EfcIBP is endowed with a unique combination of thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activity. The three-dimensional structure, solved at 0.84 Å resolution, shows that EfcIBP belongs to the IBP-1 fold family, and is organized in a right-handed β-solenoid with a triangular cross-section that forms three protein surfaces, named A, B and C faces...
March 13, 2018: FEBS Journal
T Ning, W Shao-Sheng, Y Yan-Feng, Z Yong, L I Chao-Pin
Objective To investigate whether there is Blomia tropicalis breeding in the flour warehouse of food factory in a civil aviation airport, and, if there is, to observe the morphological structure of the mite. Methods The flour samples were collected from the flour warehouse of food factory in a civil aviation airport. The breeding mites were isolated from the samples by direct microscopy and made into specimens to observe with a light microscope. Results Female Blomia tropicalis was found in the collected flour samples...
April 28, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
Saannya Sequeira, Devon Kavanaugh, Donald A MacKenzie, Tanja Šuligoj, Samuel Walpole, Charlotte Leclaire, A Patrick Gunning, Dimitrios Latousakis, William G T Willats, Jesus Angulo, Changjiang Dong, Nathalie Juge
Lactobacillus reuteri , a Gram-positive bacterial species inhabiting the gastrointestinal tract of vertebrates, displays remarkable host adaptation. Previous mutational analyses of rodent strain L. reuteri 100-23C identified a gene encoding a predicted surface-exposed serine-rich repeat protein (SRRP100-23 ) that was vital for L. reuteri biofilm formation in mice. SRRPs have emerged as an important group of surface proteins on many pathogens, but no structural information is available in commensal bacteria...
March 5, 2018: Proceedings of the National Academy of Sciences of the United States of America
Juan C Domínguez-Romero, Bienvenida Gilbert-López, Miriam Beneito-Cambra, Antonio Molina-Díaz
In this work, the implementation of Bead Injection with multicommutation-based flow systems is reported. A surface renewable chemiluminescence (CL) flow sensor is presented based on the use of CL reaction of luminol with H2 O2 . Dowex 1 × 8 beads with immobilized luminol onto them were injected in the flow system by means of a six-port rotary valve and were accommodated into a 1 mm optical glass flow cell placed just in front of the rectangular photosensor window with the same size than the cell wall. Automatic computer-controlled manipulation of both reagents and sample solutions was undertaken using a multicommutated flow system which comprises five three-way solenoid valves, a home-made electronic interface and a Java-written software...
May 15, 2018: Talanta
Tyler D R Vance, Laurie A Graham, Peter L Davies
Out of the dozen different ice-binding protein (IBP) structures known, the DUF3494 domain is the most widespread, having been passed many times between prokaryotic and eukaryotic microorganisms by horizontal gene transfer. This ~25-kDa β-solenoid domain with an adjacent parallel α-helix is most commonly associated with an N-terminal secretory signal peptide. However, examples of the DUF3494 domain preceded by tandem Bacterial Immunoglobulin-like (BIg) domains are sometimes found, though uncharacterized. Here we present one such protein (SfIBP_1) from the Antarctic bacterium Shewanella frigidimarina...
March 2, 2018: FEBS Journal
L Adamczyk, J R Adams, J K Adkins, G Agakishiev, M M Aggarwal, Z Ahammed, N N Ajitanand, I Alekseev, D M Anderson, R Aoyama, A Aparin, D Arkhipkin, E C Aschenauer, M U Ashraf, A Attri, G S Averichev, X Bai, V Bairathi, K Barish, A Behera, R Bellwied, A Bhasin, A K Bhati, P Bhattarai, J Bielcik, J Bielcikova, L C Bland, I G Bordyuzhin, J Bouchet, J D Brandenburg, A V Brandin, D Brown, I Bunzarov, J Butterworth, H Caines, M Calderón de la Barca Sánchez, J M Campbell, D Cebra, I Chakaberia, P Chaloupka, Z Chang, N Chankova-Bunzarova, A Chatterjee, S Chattopadhyay, X Chen, J H Chen, X Chen, J Cheng, M Cherney, W Christie, G Contin, H J Crawford, S Das, L C De Silva, T G Dedovich, J Deng, A A Derevschikov, L Didenko, C Dilks, X Dong, J L Drachenberg, J E Draper, L E Dunkelberger, J C Dunlop, L G Efimov, N Elsey, J Engelage, G Eppley, R Esha, S Esumi, O Evdokimov, J Ewigleben, O Eyser, R Fatemi, S Fazio, P Federic, P Federicova, J Fedorisin, Z Feng, P Filip, E Finch, Y Fisyak, C E Flores, J Fujita, L Fulek, C A Gagliardi, D Garand, F Geurts, A Gibson, M Girard, D Grosnick, D S Gunarathne, Y Guo, A Gupta, S Gupta, W Guryn, A I Hamad, A Hamed, A Harlenderova, J W Harris, L He, S Heppelmann, S Heppelmann, A Hirsch, S Horvat, X Huang, B Huang, T Huang, H Z Huang, T J Humanic, P Huo, G Igo, W W Jacobs, A Jentsch, J Jia, K Jiang, S Jowzaee, E G Judd, S Kabana, D Kalinkin, K Kang, D Kapukchyan, K Kauder, H W Ke, D Keane, A Kechechyan, Z Khan, D P Kikoła, C Kim, I Kisel, A Kisiel, L Kochenda, M Kocmanek, T Kollegger, L K Kosarzewski, A F Kraishan, L Krauth, P Kravtsov, K Krueger, N Kulathunga, L Kumar, J Kvapil, J H Kwasizur, R Lacey, J M Landgraf, K D Landry, J Lauret, A Lebedev, R Lednicky, J H Lee, C Li, X Li, Y Li, W Li, J Lidrych, T Lin, M A Lisa, P Liu, H Liu, Y Liu, F Liu, T Ljubicic, W J Llope, M Lomnitz, R S Longacre, S Luo, X Luo, Y G Ma, L Ma, R Ma, G L Ma, N Magdy, R Majka, D Mallick, S Margetis, C Markert, H S Matis, K Meehan, J C Mei, Z W Miller, N G Minaev, S Mioduszewski, D Mishra, S Mizuno, B Mohanty, M M Mondal, D A Morozov, M K Mustafa, Md Nasim, T K Nayak, J M Nelson, M Nie, G Nigmatkulov, T Niida, L V Nogach, T Nonaka, S B Nurushev, G Odyniec, A Ogawa, K Oh, V A Okorokov, D Olvitt, B S Page, R Pak, Y Pandit, Y Panebratsev, B Pawlik, H Pei, C Perkins, P Pile, J Pluta, K Poniatowska, J Porter, M Posik, N K Pruthi, M Przybycien, J Putschke, H Qiu, A Quintero, S Ramachandran, R L Ray, R Reed, M J Rehbein, H G Ritter, J B Roberts, O V Rogachevskiy, J L Romero, J D Roth, L Ruan, J Rusnak, O Rusnakova, N R Sahoo, P K Sahu, S Salur, J Sandweiss, M Saur, J Schambach, A M Schmah, W B Schmidke, N Schmitz, B R Schweid, J Seger, M Sergeeva, R Seto, P Seyboth, N Shah, E Shahaliev, P V Shanmuganathan, M Shao, A Sharma, M K Sharma, W Q Shen, S S Shi, Z Shi, Q Y Shou, E P Sichtermann, R Sikora, M Simko, S Singha, M J Skoby, N Smirnov, D Smirnov, W Solyst, L Song, P Sorensen, H M Spinka, B Srivastava, T D S Stanislaus, M Strikhanov, B Stringfellow, A A P Suaide, T Sugiura, M Sumbera, B Summa, Y Sun, X M Sun, X Sun, B Surrow, D N Svirida, Z Tang, A H Tang, A Taranenko, T Tarnowsky, A Tawfik, J Thäder, J H Thomas, A R Timmins, D Tlusty, T Todoroki, M Tokarev, S Trentalange, R E Tribble, P Tribedy, S K Tripathy, B A Trzeciak, O D Tsai, T Ullrich, D G Underwood, I Upsal, G Van Buren, G van Nieuwenhuizen, A N Vasiliev, F Videbæk, S Vokal, S A Voloshin, A Vossen, G Wang, Y Wang, F Wang, Y Wang, J C Webb, G Webb, L Wen, G D Westfall, H Wieman, S W Wissink, R Witt, Y Wu, Z G Xiao, G Xie, W Xie, J Xu, Z Xu, Q H Xu, Y F Xu, N Xu, S Yang, Y Yang, C Yang, Q Yang, Z Ye, Z Ye, L Yi, K Yip, I-K Yoo, N Yu, H Zbroszczyk, W Zha, Z Zhang, J B Zhang, J Zhang, S Zhang, Y Zhang, X P Zhang, J Zhang, S Zhang, J Zhao, C Zhong, C Zhou, L Zhou, X Zhu, Z Zhu, M Zyzak
Rapidity-odd directed-flow measurements at midrapidity are presented for Λ, Λ[over ¯], K^{±}, K_{s}^{0}, and ϕ at sqrt[s_{NN}]=7.7, 11.5, 14.5, 19.6, 27, 39, 62.4, and 200 GeV in Au+Au collisions recorded by the Solenoidal Tracker detector at the Relativistic Heavy Ion Collider. These measurements greatly expand the scope of data available to constrain models with differing prescriptions for the equation of state of quantum chromodynamics. Results show good sensitivity for testing a picture where flow is assumed to be imposed before hadron formation and the observed particles are assumed to form via coalescence of constituent quarks...
February 9, 2018: Physical Review Letters
Qiang Guo, Bin Huang, Jingdong Cheng, Manuel Seefelder, Tatjana Engler, Günter Pfeifer, Patrick Oeckl, Markus Otto, Franziska Moser, Melanie Maurer, Alexander Pautsch, Wolfgang Baumeister, Rubén Fernández-Busnadiego, Stefan Kochanek
Huntingtin (HTT) is a large (348 kDa) protein that is essential for embryonic development and is involved in diverse cellular activities such as vesicular transport, endocytosis, autophagy and the regulation of transcription. Although an integrative understanding of the biological functions of HTT is lacking, the large number of identified HTT interactors suggests that it serves as a protein-protein interaction hub. Furthermore, Huntington's disease is caused by a mutation in the HTT gene, resulting in a pathogenic expansion of a polyglutamine repeat at the amino terminus of HTT...
February 21, 2018: Nature
Stanislawa Koronkiewicz, Mihaela Trifescu, Lech Smoczynski, Harsha Ratnaweera, Slawomir Kalinowski
The novel automatic flow system, direct-injection detector (DID) integrated with multi-pumping flow system (MPFS), dedicated for the photometric determination of orthophosphates in wastewater and freshwater samples is for the first time described. All reagents and the sample were injected simultaneously, in counter-current into the reaction-detection chamber by the system of specially selected for this purpose solenoid micro-pumps. The micro-pumps provided good precision and accuracy of the injected volumes...
February 12, 2018: Environmental Monitoring and Assessment
B A Smit, E Van Zyl, J J Joubert, W Meyer, S Prévéral, C T Lefèvre, S N Venter
Magnetotactic bacteria (MTB) have the unique ability to produce magnetic particles surrounded by a biomembrane to form the magnetosome organelle. Therefore, MTB have novel physical and magnetic properties and have consequently been used in several biotechnological applications. The magnetic properties of these microorganisms and their magnetosomes have, however, never been used for the generation of electricity as described in this letter. Comparisons were made between, firstly, the electricity generated from purified magnetosomes, MTB culture (bacterial cells with magnetosomes) and sterile, liquid growth medium (control)...
February 12, 2018: Letters in Applied Microbiology
Holger Wille, Jesús R Requena
PrPSc (scrapie isoform of the prion protein) prions are the infectious agent behind diseases such as Creutzfeldt-Jakob disease in humans, bovine spongiform encephalopathy in cattle, chronic wasting disease in cervids (deer, elk, moose, and reindeer), as well as goat and sheep scrapie. PrPSc is an alternatively folded variant of the cellular prion protein, PrPC, which is a regular, GPI-anchored protein that is present on the cell surface of neurons and other cell types. While the structure of PrPC is well studied, the structure of PrPSc resisted high-resolution determination due to its general insolubility and propensity to aggregate...
February 7, 2018: Pathogens
Pier Paolo Natali, Luigi Montalto, Fabrizio Daví, Paolo Mengucci, Andrea Ciriaco, Nicola Paone, Daniele Rinaldi
The tetragonal PbWO4 (PWO) is one of the most important scintillating crystals, being used both in the Compact Muon Solenoid (CMS) experiment at the European Organization for Nuclear Research (CERN) and in the PANDA project at the Facility for Antiproton and Ion Research (FAIR). Light yield and other relevant scintillation properties depend, among many factors, also on the crystal mechanical quality. Accordingly, a detailed knowledge of crystal piezo-optic properties is a mandatory step toward understanding elasto-optic behavior and performing crystal quality control...
February 1, 2018: Applied Optics
Morten Sales, Markus Strobl, Takenao Shinohara, Anton Tremsin, Luise Theil Kuhn, William R B Lionheart, Naeem M Desai, Anders Bjorholm Dahl, Søren Schmidt
Through the use of Time-of-Flight Three Dimensional Polarimetric Neutron Tomography (ToF 3DPNT) we have for the first time successfully demonstrated a technique capable of measuring and reconstructing three dimensional magnetic field strengths and directions unobtrusively and non-destructively with the potential to probe the interior of bulk samples which is not amenable otherwise. Using a pioneering polarimetric set-up for ToF neutron instrumentation in combination with a newly developed tailored reconstruction algorithm, the magnetic field generated by a current carrying solenoid has been measured and reconstructed, thereby providing the proof-of-principle of a technique able to reveal hitherto unobtainable information on the magnetic fields in the bulk of materials and devices, due to a high degree of penetration into many materials, including metals, and the sensitivity of neutron polarisation to magnetic fields...
February 2, 2018: Scientific Reports
Alejandro M Sevillano, Natalia Fernández-Borges, Neelam Younas, Fei Wang, Saioa R Elezgarai, Susana Bravo, Ester Vázquez-Fernández, Isaac Rosa, Hasier Eraña, David Gil, Sonia Veiga, Enric Vidal, Melissa L Erickson-Beltran, Esteban Guitián, Christopher J Silva, Romolo Nonno, Jiyan Ma, Joaquín Castilla, Jesús R Requena
Very solid evidence suggests that the core of full length PrPSc is a 4-rung β-solenoid, and that individual PrPSc subunits stack to form amyloid fibers. We recently used limited proteolysis to map the β-strands and connecting loops that make up the PrPSc solenoid. Using high resolution SDS-PAGE followed by epitope analysis, and mass spectrometry, we identified positions ~116/118, 133-134, 141, 152-153, 162, 169 and 179 (murine numbering) as Proteinase K (PK) cleavage sites in PrPSc. Such sites likely define loops and/or borders of β-strands, helping us to predict the threading of the β-solenoid...
January 31, 2018: PLoS Pathogens
Taiki Yoda, Takehito Yokoyama, Shuichi Murakami
We theoretically study current-induced orbital magnetization in a chiral crystal. This phenomenon is an orbital version of the Edelstein effect. We propose an analogy between the current-induced orbital magnetization and an Amp\`ere field in a solenoid in classical electrodynamics. In order to quantify this effect, we define a dimensionless parameter from the response coefficient relating a current density with an orbital magnetization. This dimensionless parameter can be regarded as a number of turns within a unit cell when the crystal is regarded as a solenoid, and it represents how ``chiral'' the crystal is...
January 26, 2018: Nano Letters
Taehoon H Kim, Young Ki Hahn, Jungmin Lee, Danny van Noort, Minseok S Kim
As paper-based diagnostics has become predominantly driven by more advanced microfluidic technology, many of the research efforts are still focused on developing reliable and versatile fluidic control devices, apart from improving sensitiv-ity and reproducibility. In this paper, we introduce a novel and robust paper fluidic control system enabling versatile flu-idic control. The system comprises a linear push-pull solenoid and an Arduino Uno microcontroller. The precisely con-trolled pressure exerted on the paper stops the flow...
January 24, 2018: Analytical Chemistry
Verma Subhash, Sodhi Monika, Salwan Richa, Shekhar Chander, Singh Geetanjali, Sharma Mandeep
Distribution of polymorphism in TLR2 gene was studied in Pahari (Indian non-descript indigenous breed) cattle of Himachal Pradesh. The complete sequence of TLR2 gene consisted of 136bp 5'-UTR, 2355bp CDS and 1316bp 3'UTR. The phylogenetic analysis revealed clustering of Indian Zebu with Bos taurus as the nearest neighbour. The nucleotide sequences of TLR2 revealed the occurrence of 19 SNPs including 47% non-synonymous and 53% synonymous SNPs within TLR domain among all bovines. The dS/dN ratio (ω)<1 at polymorphic sites revealed purifying selection and thus, indicating the presence of highly conserved domains...
December 15, 2017: Research in Veterinary Science
Thomas Meier, Nan Wang, Dario Mager, Jan G Korvink, Sylvain Petitgirard, Leonid Dubrovinsky
A new pathway to nuclear magnetic resonance (NMR) spectroscopy for picoliter-sized samples (including those kept in harsh and extreme environments, particularly in diamond anvil cells) is introduced, using inductively coupled broadband passive electromagnetic lenses, to locally amplify the magnetic field at the isolated sample, leading to an increase in sensitivity. The lenses are adopted for the geometrical restrictions imposed by a toroidal diamond indenter cell and yield signal-to-noise ratios at pressures as high as 72 GPa at initial sample volumes of only 230 pl...
December 2017: Science Advances
Bernard C Collins, Hiro Nakahara, Sharmistha Acharya, Max D Cooper, Brantley R Herrin, Ian A Wilson
Variable lymphocyte receptors (VLRs), the leucine-rich repeat (LRR)-based antigen receptors of jawless fish, have great utility in a wide variety of biochemical and biological applications, similar to classical Ig-based antibodies. VLR-based reagents may be particularly useful when traditional antibodies are not available. An anti-idiotype lamprey VLR, VLR39, has previously been identified that recognizes the heavy-chain CDR3 of the B-cell receptor (BCR) of a leukemic clone from a patient with chronic lymphocytic leukemia (CLL)...
December 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
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