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https://www.readbyqxmd.com/read/27858026/a-portable-microfluidic-system-for-rapid-measurement-of-the-erythrocyte-sedimentation-rate
#1
Ziya Isiksacan, Ozcan Erel, Caglar Elbuken
The erythrocyte sedimentation rate (ESR) is a frequently used 30 min or 60 min clinical test for screening of several inflammatory conditions, infections, trauma, and malignant diseases, as well as non-inflammatory conditions including prostate cancer and stroke. Erythrocyte aggregation (EA) is a physiological process where erythrocytes form face-to-face linear structures, called rouleaux, at stasis or low shear rates. In this work, we proposed a method for ESR measurement from EA. We developed a microfluidic opto-electro-mechanical system, using which we experimentally showed a significant correlation (R(2) = 0...
November 18, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27856162/generation-of-digitized-microfluidic-filling-flow-by-vent-control
#2
Junghyo Yoon, Eundoo Lee, Jaehoon Kim, Sewoon Han, Seok Chung
Quantitative microfluidic point-of-care testing has been translated into clinical applications to support a prompt decision on patient treatment. A nanointerstice-driven filling technique has been developed to realize the fast and robust filling of microfluidic channels with liquid samples, but it has failed to provide a consistent filling time owing to the wide variation in liquid viscosity, resulting in an increase in quantification errors. There is a strong demand for simple and quick flow control to ensure accurate quantification, without a serious increase in system complexity...
October 28, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27827923/the-development-of-a-portable-spr-bioanalyzer-for-sensitive-detection-of-escherichia-coli-o157-h7
#3
Shun Wang, Jiufeng Xie, Min Jiang, Keke Chang, Ruipeng Chen, Liuzheng Ma, Juanhua Zhu, Qingqian Guo, Haifeng Sun, Jiandong Hu
The purpose of this study was to develop a portable surface plasmon resonance (SPR) bioanalyzer for the sensitive detection of Escherichia coli O157:H7 in comparison with an enzyme-linked immunosorbent assay (ELISA). The experimental setup mainly consisted of an integrated biosensor and a homemade microfluidic cell with a three-way solenoid valve. In order to detect Escherichia coli O157:H7 using the SPR immunoassay, 3-mercaptopropionic acid (3-MPA) was chemisorbed onto a gold surface via covalent bond for the immobilization of biological species...
November 4, 2016: Sensors
https://www.readbyqxmd.com/read/27817129/the-use-of-stepper-motor-controlled-proportional-valve-for-fio2-calculation-in-the-ventilator-and-its-control-with-fuzzy-logic
#4
Adem Gölcük, İnan Güler
This article proposes the employment of a proportional valve that can calculate the amount of oxygen in the air to be given to patient in accordance with the amount of FiO2 which is set from the control menu of the ventilation device. To actualize this, a stepper motor-controlled proportional valve was used. Two counts of valves were employed in order to control the gases with 2 bar pressure that came from both the oxygen and medical air tanks. Oxygen and medical air manometers alongside the pressure regulators were utilized to perform this task...
January 2017: Journal of Medical Systems
https://www.readbyqxmd.com/read/27802759/design-and-modeling-of-magnetically-driven-electric-field-sensor-for-non-contact-dc-voltage-measurement-in-electric-power-systems
#5
Decai Wang, Ping Li, Yumei Wen
In this paper, the design and modeling of a magnetically driven electric-field sensor for non-contact DC voltage measurement are presented. The magnetic drive structure of the sensor is composed of a small solenoid and a cantilever beam with a cylindrical magnet mounted on it. The interaction of the magnet and the solenoid provides the magnetic driving force for the sensor. Employing magnetic drive structure brings the benefits of low driving voltage and large vibrating displacement, which consequently results in less interference from the drive signal...
October 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27802742/short-period-cyclic-loading-system-for-in-situ-x-ray-observation-of-anelastic-properties-at-high-pressure
#6
Takashi Yoshino, Daisuke Yamazaki, Yoshinori Tange, Yuji Higo
To determine the anelastic properties of materials of the Earth's interior, a short-period cyclic loading system was installed for in situ X-ray radiographic observation under high pressure to the multi-anvil deformation DIA press at the bending magnet beam line BL04B1 at SPring-8. The hydraulic system equipped with a piston controlled by a solenoid was designed so as to enable producing smooth sinusoidal stress in a wide range of oscillation period from 0.2 to 100 s and generating variable amplitudes. Time resolved X-ray radiography imaging of the sample and reference material provides their strain as a function of time during cyclic loading...
October 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27796327/gigagauss-solenoidal-magnetic-field-inside-bubbles-excited-in-under-dense-plasma
#7
Zs Lécz, I V Konoplev, A Seryi, A Andreev
This paper proposes a novel and effective method for generating GigaGauss level, solenoidal quasi-static magnetic fields in under-dense plasma using screw-shaped high intensity laser pulses. This method produces large solenoidal fields that move with the driving laser pulse and are collinear with the accelerated electrons. This is in contrast with already known techniques which rely on interactions with over-dense or solid targets and generates radial or toroidal magnetic field localized at the stationary target...
October 31, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27782824/the-c-terminal-domain-of-tpx2-is-made-of-alpha-helical-tandem-repeats
#8
Luis Sanchez-Pulido, Laurent Perez, Steffen Kuhn, Isabelle Vernos, Miguel A Andrade-Navarro
BACKGROUND: TPX2 (Targeting Protein for Xklp2) is essential for spindle assembly, activation of the mitotic kinase Aurora A and for triggering microtubule nucleation. Homologs of TPX2 in Chordata and plants were previously identified. Currently, proteins of the TPX2 family have little structural information and only small parts are covered by defined protein domains. METHODS: We have used computational sequence analyses and structural predictions of proteins of the TPX2 family, supported with Circular Dichroism (CD) measurements...
October 26, 2016: BMC Structural Biology
https://www.readbyqxmd.com/read/27782584/a-kind-of-fast-shutter-for-z-pinch-diagnosis-device
#9
Liangping Wang, Xinjun Zhang, Tieping Sun, Wentin Mao
A kind of fast shutter for protecting the diagnosis devices in Z pinch experiments is introduced in this paper. The shutter is composed of a pulling rod, a magnetic core, and a solenoid. Different from the traditional coils which were used at the voltage of 220 V, the solenoid we used must endure the high voltage of 5-10 kV and the deformation which maybe caused by the 5-10 T intense magnetic field. A creative configuration for the solenoid is developed including the winding guide, insulating sleeve, and stainless-steel sleeve...
September 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27703853/a-direct-co2-control-system-for-ocean-acidification-experiments-testing-effects-on-the-coralline-red-algae-phymatolithon-lusitanicum
#10
Laura Sordo, Rui Santos, Joao Reis, Alona Shulika, Joao Silva
Most ocean acidification (OA) experimental systems rely on pH as an indirect way to control CO2. However, accurate pH measurements are difficult to obtain and shifts in temperature and/or salinity alter the relationship between pH and pCO2. Here we describe a system in which the target pCO2 is controlled via direct analysis of pCO2 in seawater. This direct type of control accommodates potential temperature and salinity shifts, as the target variable is directly measured instead of being estimated. Water in a header tank is permanently re-circulated through an air-water equilibrator...
2016: PeerJ
https://www.readbyqxmd.com/read/27606840/the-structural-architecture-of-an-infectious-mammalian-prion-using-electron-cryomicroscopy
#11
Ester Vázquez-Fernández, Matthijn R Vos, Pavel Afanasyev, Lino Cebey, Alejandro M Sevillano, Enric Vidal, Isaac Rosa, Ludovic Renault, Adriana Ramos, Peter J Peters, José Jesús Fernández, Marin van Heel, Howard S Young, Jesús R Requena, Holger Wille
The structure of the infectious prion protein (PrPSc), which is responsible for Creutzfeldt-Jakob disease in humans and bovine spongiform encephalopathy, has escaped all attempts at elucidation due to its insolubility and propensity to aggregate. PrPSc replicates by converting the non-infectious, cellular prion protein (PrPC) into the misfolded, infectious conformer through an unknown mechanism. PrPSc and its N-terminally truncated variant, PrP 27-30, aggregate into amorphous aggregates, 2D crystals, and amyloid fibrils...
September 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27605033/thin-film-based-semi-active-resonant-marker-design-for-low-profile-interventional-cardiovascular-mri-devices
#12
Engin Baysoy, Dursun Korel Yildirim, Cagla Ozsoy, Senol Mutlu, Ozgur Kocaturk
OBJECTIVES: A new microfabrication method to produce low profile radio frequency (RF) resonant markers on catheter shafts was developed. A semi-active RF resonant marker incorporating a solenoid and a plate capacitor was constructed on the distal shaft of a 5 Fr guiding catheter. The resulting device can be used for interventional cardiovascular MRI procedures. MATERIALS AND METHODS: Unlike current semi-active device visualization techniques that require rigid and bulky analog circuit components (capacitor and solenoid), we fabricated a low profile RF resonant marker directly on guiding the catheter surface by thin film metal deposition and electroplating processes using a modified physical vapor deposition system...
September 7, 2016: Magma
https://www.readbyqxmd.com/read/27587135/electromagnetic-tweezers-with-independent-force-and-torque-control
#13
Chang Jiang, Troy A Lionberger, Diane M Wiener, Edgar Meyhofer
Magnetic tweezers are powerful tools to manipulate and study the mechanical properties of biological molecules and living cells. In this paper we present a novel, bona fide electromagnetic tweezer (EMT) setup that allows independent control of the force and torque applied via micrometer-sized magnetic beads to a molecule under study. We implemented this EMT by combining a single solenoid that generates force (f-EMT) with a set of four solenoids arranged into a symmetric quadrupole to generate torque (τ-EMT)...
August 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27573845/synthetic-beta-solenoid-proteins-with-the-fragment-free-computational-design-of-a-beta-hairpin-extension
#14
James T MacDonald, Burak V Kabasakal, David Godding, Sebastian Kraatz, Louie Henderson, James Barber, Paul S Freemont, James W Murray
The ability to design and construct structures with atomic level precision is one of the key goals of nanotechnology. Proteins offer an attractive target for atomic design because they can be synthesized chemically or biologically and can self-assemble. However, the generalized protein folding and design problem is unsolved. One approach to simplifying the problem is to use a repetitive protein as a scaffold. Repeat proteins are intrinsically modular, and their folding and structures are better understood than large globular domains...
September 13, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27539394/improved-flow-modulator-construction-for-gc-%C3%A3-gc-with-quadrupole-mass-spectrometry
#15
Martin Ston, Radomir Cabala, Vaclav Matej Bierhanzl, Jan Krajicek, Zuzana Bosakova
Improvement and testing of a flow modulator for the application in comprehensive two-dimensional gas chromatography separations is the subject of the presented paper. This improved setup constructed from two independent capillary branches each consisting of a pressure regulator, a pressure sensor, a two-way solenoid valve and a microfluidic T-connector, allows an independent and easy settings of the pressures and flow velocities in the modulator and provides system flexibility in an operation without need of any component exchange...
August 18, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27503934/magnetic-resonance-guided-nasogastric-feeding-tube-placement-for-neonates-a-preclinical-study
#16
Barret Daniels, Christopher Ireland, Steven Kraus, John Racadio, Nicole Hilvert, Richard Dunn, Charles Dumoulin
BACKGROUND: Establishing postnatal nutrition delivery is challenging in neonates with immature sucking and swallowing ability. Enteral feeding is the gold standard for such patients, but their small size and fragility present challenges in nasogastric (NG) feeding tube placement. Feeding tubes are typically placed with x-ray guidance, which provides minimal soft tissue contrast and exposes the baby to ionizing radiation. This research investigates magnetic resonance (MR) guidance of NG feeding tube placement in neonates to provide improved soft tissue visualization without ionizing radiation...
August 8, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27478291/quantification-of-the-oxygen-uptake-rate-in-a-dissolved-oxygen-controlled-oscillating-jet-driven-microbioreactor
#17
Timothy V Kirk, Marco Pc Marques, Anand N Pallipurath Radhakrishnan, Nicolas Szita
BACKGROUND: Microbioreactors have emerged as a new tool for early bioprocess development. The technology has advanced rapidly in the last decade and obtaining real-time quantitative data of process variables is nowadays state of the art. In addition, control over process variables has also been achieved. The aim of this study was to build a microbioreactor capable of controlling dissolved oxygen (DO) concentrations and to determine oxygen uptake rate in real time. RESULTS: An oscillating jet driven, membrane-aerated microbioreactor was developed without comprising any moving parts...
March 2016: Journal of Chemical Technology and Biotechnology
https://www.readbyqxmd.com/read/27476662/discontinuous-atmospheric-pressure-interface-for-mass-spectrometry-using-a-solenoid-pulse-valve
#18
Dilshadbek T Usmanov, Kenzo Hiraoka
RATIONALE: For the development of on-site mass spectrometry for security and safety, point-of-care analysis, etc., the gas volume introduced into the vacuum should be reduced to a minimum. To cope with this demand, a discontinuous atmospheric pressure interface using a solenoid pulse valve was developed. METHODS: The sample gas was introduced discontinuously into the ionization cell with a volume of 0.17 cm(3) . The sampled gas in the cell was ionized by an alternating current (ac) corona discharge...
August 30, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27472632/scattering-detection-of-a-solenoidal-poynting-vector-field
#19
Shima Fardad, Alessandro Salandrino, Akbar Samadi, Matthias Heinrich, Zhigang Chen, Demetrios N Christodoulides
The Poynting vector S plays a central role in electrodynamics as it is directly related to the power and the momentum carried by an electromagnetic wave. In the presence of multiple electromagnetic waves with different polarizations and propagation directions, the Poynting vector may exhibit solenoidal components which are not associated to any power flow. Here, we demonstrate theoretically and experimentally that the presence of such solenoidal components has physical consequences, and it is not a mere artifact of the gauge invariance of S...
August 1, 2016: Optics Letters
https://www.readbyqxmd.com/read/27419870/architecture-of-the-90s-pre-ribosome-a-structural-view-on-the-birth-of-the-eukaryotic-ribosome
#20
Markus Kornprobst, Martin Turk, Nikola Kellner, Jingdong Cheng, Dirk Flemming, Isabelle Koš-Braun, Martin Koš, Matthias Thoms, Otto Berninghausen, Roland Beckmann, Ed Hurt
The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of ∼70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 β-propellers and large α-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA...
July 14, 2016: Cell
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