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P B Wagle, N A Jambhekar, R Kumar, K Prabhash, C S Pramesh, S B Desai, V Noronha, G Karimundackal, A Shah, A Joshi, S G Laskar, S Jiwnani, T Pai, J P Agarwal
INTRODUCTION: Testing for echinoderm microtubule-associated protein-like 4 (EML4) anaplastic lymphoma kinase (ALK) translocation by fluorescence in situ hybridization (FISH) is well established whereas the Food and Drug Administration (FDA) ALK immunohistochemical (IHC) test is relatively new. AIMS AND OBJECTIVE: The aim of this study is to compare FDA-approved ALK IHC test (D5F3 clone) with the standard ALK FISH test. MATERIALS AND METHODS: A validation and a test arm with 100 and 200 cases of Formalin-Fixed, Paraffin-embedded blocks of lung adenocarcinoma, respectively, comprised the material...
January 2017: Indian Journal of Cancer
Wenjun Zhang, Antony Hubbard, Tobin Jones, Adriana Racolta, Srabani Bhaumik, Nick Cummins, Liping Zhang, Karl Garsha, Frank Ventura, Mark R Lefever, Zhenqiang Lu, John K Hurley, William A Day, Lidija Pestic-Dragovich, Larry E Morrison, Lei Tang
The ability to simultaneously visualize the presence, abundance, location and functional state of many targets in cells and tissues has been described as a true next-generation approach in immunohistochemistry (IHC). A typical requirement for multiplex IHC (mIHC) is the use of different animal species for each primary (1°Ab) and secondary (2°Ab) antibody pair. Although 1°Abs from different species have been used with differently labeled species-specific 2°Abs, quite often the appropriate combination of antibodies is not available...
July 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
Yoshitaro Saito, Kazuhiro Imai, Ryuta Nakamura, Hiroshi Nanjo, Kaori Terata, Hayato Konno, Yoichi Akagami, Yoshihiro Minamiya
Human epidermal growth factor receptor 2 (HER2)-targeted agents are an effective approach to treating HER2-positive breast cancer patients. However, the lack of survival benefit in HER2-negative patients as well as the toxic effects and high cost of the drugs highlight the need for accurate and prompt assessment of HER2 status. Our aim was to evaluate the clinical utility of a novel rapid dual in-situ hybridization (RISH) method developed to facilitate hybridization. The method takes advantage of the non-contact mixing effect of an alternating current (AC) electric field...
2016: Scientific Reports
Diana Taheri, David J Zahavi, Maria Del Carmen Rodriguez, Abdelrazak Meliti, Neda Rezaee, Raluca Yonescu, Bernardo F P Ricardo, Shahaboddin Dolatkhah, Yi Ning, Justin A Bishop, George J Netto, Rajni Sharma
Inflammatory myofibroblastic tumor (IMT) is a rare neoplasm. Approximately 50 % of IMTs show an anaplastic lymphoma kinase (ALK) gene fusion resulting in ALK overexpression on immunohistochemistry (IHC). A novel anti-ALK monoclonal antibody (D5F3) has been suggested to be of superior sensitivity to the ALK1 antibody which is currently used. We compared the performance of D5F3 in detecting ALK protein expression in IMTs from various anatomic sites compared to the currently utilized ALK1. We selected 25 IMTs from our surgical pathology files (2005-2015)...
September 2016: Virchows Archiv: An International Journal of Pathology
Carol C Cheung, Clive R Taylor, Emina E Torlakovic
Appropriate controls are critical for the correct interpretation of immunohistochemistry (IHC) assays and help to detect unsuccessful/suboptimal slides. We performed an audit of slides that were designated as being "failed" by the IHC laboratory (ie, laboratory-failed slides) of a large North American oncology and transplant center. All slides were run with on-slide controls. The study included analysis of only those failed slides where staining of both internal and external controls were unsuccessful/suboptimal in a period of 65 days...
May 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
Mirta Gamez
No abstract text is available yet for this article.
October 2015: MLO: Medical Laboratory Observer
Mogens Vyberg, Søren Nielsen
Despite extensive use of immunohistochemistry (IHC) for decades, lack of standardization remains a major problem, even aggravated in the era of targeted therapy. Nordic Immunohistochemical Quality Control (NordiQC) is an international academic proficiency testing (PT) program established in 2003 primarily aimed at assessing the analytical phases of the laboratory IHC quality. About 700 laboratories from 80 countries are currently participating. More than 30,000 IHC slides have been evaluated during 2003-2015...
January 2016: Virchows Archiv: An International Journal of Pathology
Eric Piaton, Monique Fabre, Isabelle Goubin-Versini, Marie-Françoise Bretz-Grenier, Monique Courtade-Saïdi, Serge Vincent, Geneviève Belleannée, Françoise Thivolet, Jean Boutonnat, Hervé Debaque, Jocelyne Fleury-Feith, Philippe Vielh, Béatrix Cochand-Priollet, Caroline Egelé, Jean-Pierre Bellocq, Jean-François Michiels
May-Grünwald-Giemsa (MGG) stain is a Romanowsky-type, polychromatic stain as those of Giemsa, Leishman and Wright. Apart being the reference method of haematology, it has become a routine stain of diagnostic cytopathology for the study of air-dried preparations (lymph node imprints, centrifuged body fluids and fine needle aspirations). In the context of their actions of promoting the principles of quality assurance in cytopathology, the French Association for Quality Assurance in Anatomic and Cytologic Pathology (AFAQAP) and the French Society of Clinical Cytology (SFCC) conducted a proficiency test on MGG stain in 2013...
August 2015: Annales de Pathologie
Eberson Damião Dos Santos de Bitencourt, Carlos Franco Voegeli, Gabriela Dos Santos Onzi, Sara Cardoso Boscato, Carine Ghem, Terezinha Munhoz
BACKGROUND: The speed and quality of information have become essential items in the release of laboratory reports. The Sysmex(®)SP1000-I device has been developed to prepare and stain smear slides. However, for a device to be cleared for use in the laboratory routine it must pass through a validation process. OBJECTIVE: To evaluate the performance and reliability of the Sysmex(®) SP-1000i slide preparer-stainer incorporated into the routine of a hospital laboratory in Porto Alegre...
2013: Revista Brasileira de Hematologia e Hemoterapia
Jennifer S Ko, Steven D Billings, Christopher P Lanigan, Darya Buehler, Anthony P Fernandez, Raymond R Tubbs
BACKGROUND: MYC amplification occurs in post-radiation and chronic lymphedema-associated secondary angiosarcoma and some primary angiosarcomas. In this study, we tested the ability of automated dual-color dual-hapten in situ hybridization (DISH) staining to discriminate secondary angiosarcoma from radiation-associated atypical vascular lesions (AVL), and to correlate with fluorescence in situ hybridization (FISH) for MYC amplification. METHODS: Cases of secondary angiosarcoma, including 11 biopsies and 3 excisions from 11 patients, and 5 AVL biopsies from 5 patients, were examined by FISH and DISH...
March 2014: Journal of Cutaneous Pathology
W Brown, M Keeney, B D Hedley
INTRODUCTION: Despite the advancements in instrumentation within hematology laboratories, there is still a need for review of a peripheral blood film (PBF). For a thorough PBF evaluation, it is critical that a well spread and stained film is available. METHODS: In this study, we evaluated an automatic slide maker/stainer (DxH-SMS, Beckman Coulter) compared with manually prepared blood films on 124 normal and abnormal samples. The primary goal of the study was to determine whether or not the DxH-SMS was able to consistently and reproducibly prepare and stain blood films of exemplary quality, without carryover between specimens...
April 2014: International Journal of Laboratory Hematology
Spasenija Savic, Beata Bode, Joachim Diebold, Ivo Tosoni, Audrey Barascud, Betty Baschiera, Bruno Grilli, Michelle Herzog, Ellen Obermann, Lukas Bubendorf
INTRODUCTION: Lung cancer is often diagnosed by cytology, necessitating predictive molecular marker analyses on cytological specimens. The gold standard for detection of predictive anaplastic lymphoma kinase (ALK)-rearrangements is fluorescence in situ hybridization (FISH), but FISH is both expensive and often challenging to interpret. The aim of our study was to investigate the accuracy of ALK immunocytochemistry (ICC) on cytological specimens of non-small-cell lung cancers (NSCLCs)...
August 2013: Journal of Thoracic Oncology
Robert Dondelinger
No abstract text is available yet for this article.
March 2013: Biomedical Instrumentation & Technology
Angie Cahill, Jeff Pearson
Methods relative to the staining of tissues using hematoxylin and eosin (H&E) have largely not evolved beyond linear batch staining processes. The batching of slides in the histopathology laboratory inherently leads to the sharing of the various reagents among those specimens being processed through the baths. Studies analyzing the effects of reagent sharing during the common H&E linear staining method are limited. This study assessed rates of extraneous tissue contamination found in selected stainer bath containers from the deparaffinization portion of the H&E linear staining procedure...
September 2012: Journal of Histotechnology
Karen Lynn
No abstract text is available yet for this article.
July 2011: MLO: Medical Laboratory Observer
M Hur, J-H Cho, H Kim, M-H Hong, H-W Moon, Y-M Yun, J Q Kim
INTRODUCTION: The validation of automated hematology analyzer results by manual slide review (MSR) is currently an inevitable work process in clinical hematology laboratories. The laboratory workload would be optimized if the requirement for MSR could be reduced without compromising patient care. We investigated whether slide-making rates would be different between two hematology analyzers, which were paired with their own automated slide makers/stainers: Sysmex XE-2100 with SP-1000i (Sysmex, Kobe, Japan) and ABX Pentra DX120 with SPS evolution (ABX-Horiba, Montpellier, France)...
August 2011: International Journal of Laboratory Hematology
Vu Hong Thang, Edneia Tani, Hemming Johansson, Jan Adolfsson, Kamilla Krawiec, Ta Thanh Van, Lambert Skoog
BACKGROUND: The aim of the present study was to compare both estrogen (ER) and progesterone receptor (PgR) content in operable breast cancers from Vietnamese and Swedish patients. MATERIAL AND METHODS: Primary breast cancer tissues were randomly selected from 249 Vietnamese patients treated in Hanoi, Vietnam between 2002 and 2004 and 1 257 Swedish patients treated in Stockholm, Sweden between 2002 and 2003. Clinical information was available for all patients in the study...
April 2011: Acta Oncologica
Carla Fontana, Marco Favaro, Dolores Limongi, Jana Pivonkova, Cartesio Favalli
BACKGROUND: The first step in routine microbiology laboratory procedures is the collection and safe transportation of swab samples. This can be accomplished using ESwab Collection and Transport System (Copan Italia, Brescia -Italy). The aim of the present study was to compare the results of microscopic examination of gram stain smears prepared directly from clinical specimens, collected and transported in the ESwab, with those obtained using Amies Agar gel Transystem without charcoal (Copan)...
2009: BMC Research Notes
L Skagias, E Politi, A Karameris, D Sambaziotis, A Archondakis, O Vasou, A Ntinis, F Michalopoulou, I Moreas, H Koutselini, E Patsouris
PURPOSE: This study was conducted to evaluate the quantitative assessment of HER2/neu immunohistochemical expression in urothelial bladder cancer in order to determine its prognostic significance. MATERIALS AND METHODS: Archival tumor tissue from 80 patients with primary urothelial carcinoma were analysed for HER2/neu immunohistochemical expression. A highly reproducible standardized procedure on a Bond-X automated slide stainer was used. RESULTS: HER2 protein was overexpressed in 41 of 80 patients (51...
July 2009: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
Robin W Allison, Karen E Velguth
BACKGROUND: Romanowsky stains are used routinely by veterinary clinical pathology laboratories for cytologic and blood film evaluations. Automated stainers are available for both aqueous and methanolic Romanowsky stains. Mast cell granules and canine distemper virus inclusions are known to stain differently by these 2 methods, but we have noticed differences in the staining characteristics of other granulated cells. OBJECTIVE: The aim of this study was to investigate and document the variable appearance of basophils and large granular lymphocytes in blood films stained using aqueous and methanolic Romanowsky methods...
March 2010: Veterinary Clinical Pathology
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