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https://www.readbyqxmd.com/read/28734987/bioavailable-and-free-25-hydroxyvitamin-d-and-vitamin-d-binding-protein-in-polycystic-ovary-syndrome-relationships-with-obesity-and-insulin-resistance
#1
Negar Naderpoor, Soulmaz Shorakae, Sally K Abell, Aya Mousa, Anju E Joham, Lisa J Moran, Nigel K Stepto, Poli Mara Spritzer, Helena J Teede, Barbora de Courten
Polycystic ovary syndrome (PCOS) is a common condition characterised by both reproductive and metabolic features (obesity, insulin resistance, diabetes risk). Some evidence suggests that women with PCOS have lower vitamin D levels compared to healthy controls. Vitamin D binding protein (DBP) is the main carrier of vitamin D in circulation and plays an important role in regulating vitamin D concentration and bioavailability for target tissues. To our knowledge, no previous studies have examined DBP, bioavailable and free 25-hydroxyvitamin D (25(OH)D) in women with PCOS...
July 19, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28733958/the-study-of-postmortem-human-synaptosomes-for-understanding-alzheimer-s-disease-and-other-neurological-disorders-a-review
#2
REVIEW
Jia-Fong Jhou, Hwan-Ching Tai
Synaptic dysfunction is thought to play important roles in the pathophysiology of many neurological diseases, including Alzheimer's disease, Parkinson's disease, and schizophrenia. Over the past few decades, there have been systematic efforts to collect postmortem brain tissues via autopsies, leading to the establishment of dozens of human brain banks around the world. From cryopreserved human brain tissues, it is possible to isolate detached-and-resealed synaptic terminals termed synaptosomes, which remain metabolically and enzymatically active...
July 2017: Neurology and Therapy
https://www.readbyqxmd.com/read/28733347/ultrastructural-characteristics-and-immune-profile-of-equine-mscs-from-fetal-adnexa
#3
Eleonora Iacono, Luisa Pascucci, Barbara Rossi, Cinzia Bazzucchi, Aliai Lanci, Monica Ceccoli, Barbara Merlo
Both in human and equine species, mesenchymal stem cells (MSCs) from amniotic membrane (AM) and Wharton's jelly (WJ), may be particularly useful for immediate use or in later stages of life, after cryopreservation in cell bank. The aim of this study was to compare equine AM- and WJ-MSCs in vitro features, that may be relevant for their clinical employment. MSCs were more easily isolated from WJ, even if MSCs derived from AM exhibited more rapid proliferation (P<0.05). Osteogenic and chondrogenic differentiation were more prominent in MSCs derived from WJ...
July 21, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28732082/osteosarcoma-enters-a-post-genomic-era-with-in-silico-opportunities-generation-of-the-high-dimensional-database-for-facilitating-sarcoma-biology-research-a-report-from-the-children-s-oncology-group-and-the-quadw-foundation
#4
Jason Glover, Tsz-Kwong Man, Donald A Barkauskas, David Hall, Tanya Tello, Mary Beth Sullivan, Richard Gorlick, Katherine Janeway, Holcombe Grier, Ching Lau, Jeffrey A Toretsky, Scott C Borinstein, Chand Khanna, Timothy M Fan
The prospective banking of osteosarcoma tissue samples to promote research endeavors has been realized through the establishment of a nationally centralized biospecimen repository, the Children's Oncology Group (COG) biospecimen bank located at the Biopathology Center (BPC)/Nationwide Children's Hospital in Columbus, Ohio. Although the physical inventory of osteosarcoma biospecimens is substantive (>15,000 sample specimens), the nature of these resources remains exhaustible. Despite judicious allocation of these high-value biospecimens for conducting sarcoma-related research, a deeper understanding of osteosarcoma biology, in particular metastases, remains unrealized...
2017: PloS One
https://www.readbyqxmd.com/read/28719337/determination-of-some-heavy-metals-in-mastacembelus-mastacembelus-banks-solander-1794-in-terms-of-public-health
#5
M Eroğlu, M Düşükcan, Ö Canpolat, M Çalta, D Şen
In this study, the accumulation of some heavy metals in spiny eel (Mastacembelus mastacembelus Banks and Solander, 1794) living in Karakaya Dam Lake was determined and human health risk of this fish when consumed as food was examined. For this purpose, the amounts of copper (Cu), chromium (Cr), cadmium(Cd), iron (Fe), zinc (Zn) in water samples and in the muscle tissues of the fishes were determined. The amounts of heavy metals showed differences in the muscle tissues of Mastacembelus mastacembelus according to weight, length, sex and age groups of fish...
May 20, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28716597/cells-isolated-from-cryopreserved-dental-follicle-display-similar-characteristics-to-cryopreserved-dental-follicle-cells
#6
Hefeng Yang, Jie Li, JingJing Sun, Weihua Guo, Hui Li, Jinglong Chen, Yu Hu, Weidong Tian, Song Li
Dental follicle tissue is a promising resource of mesenchymal stem cells for cytotherapeutic approaches and tissue engineering applications. There are two procedures for banking of human dental follicle stem cells have been reported. Conventional method requires cell isolation, expansion and immediate cryopreservation. Whereas dental follicle stem cells can be isolated from cryopreserved dental follicle fragments. The aim of this study was to compare the characteristics of dental follicle cells isolated from cryopreserved fragments (DFCs-CF) with dental follicle cells recovered from cryopreserved cells (DFCs-CC)...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28712097/fertility-preservation-in-young-adult-patients-with-cancer
#7
Joanne Frankel Kelvin
More than 60,000 young adults are diagnosed with cancer each year in the United States. Many will not have started or completed their families at the time of diagnosis, and reproductive concerns are common. Practice guidelines from a number of professional organizations, including the American Society of Clinical Oncology, highlight the need for oncology clinicians to address fertility with their at-risk patients by discussing options for fertility preservation. Fertility preservation options for men include sperm banking (through manual stimulation, electroejaculation, and testicular sperm extraction) and testicular shielding...
July 15, 2017: Oncology (Williston Park, NY)
https://www.readbyqxmd.com/read/28701069/current-success-and-efficiency-of-autologous-ovarian-transplantation-a-meta-analysis
#8
Fernanda Pacheco, Kutluk Oktay
CONTEXT: Ovarian cryopreservation followed by autotransplantation is still considered an experimental strategy for fertility preservation (FP) mainly because the success rates are unknown. OBJECTIVE: To determine cohort epidemiologic characteristics and success rates of autologous ovarian tissue transplantation (OTT) with previously cryopreserved tissue. MATERIALS AND METHODS: Literature review from 1999 to October 1, 2016. Additional cases were retrieved from meeting abstracts and own database...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28700536/fully-implantable-battery-free-wireless-optoelectronic-devices-for-spinal-optogenetics
#9
Vijay K Samineni, Jangyeol Yoon, Kaitlyn E Crawford, Yu Ra Jeong, Kajanna C McKenzie, Gunchul Shin, Zhaoqian Xie, Saranya S Sundaram, Yuhang Li, Min Young Yang, Jeonghyun Kim, Di Wu, Yeguang Xue, Xue Feng, Yonggang Huang, Aaron D Mickle, Anthony Banks, Jeong Sook Ha, Judith P Golden, John A Rogers, Robert W Gereau
The advent of optogenetic tools has allowed unprecedented insights into the organization of neuronal networks. Although recently developed technologies have enabled implementation of optogenetics for studies of brain function in freely moving, untethered animals, wireless powering and device durability pose challenges in studies of spinal cord circuits where dynamic, multidimensional motions against hard and soft surrounding tissues can lead to device degradation. We demonstrate here a fully implantable optoelectronic device powered by near-field wireless communication (NFC) technology, with a thin and flexible open-architecture that provides excellent mechanical durability, robust sealing against biofluid penetration and fidelity in wireless activation, thereby allowing for long-term optical stimulation of the spinal cord without constraint on the natural behaviors of the animals...
June 8, 2017: Pain
https://www.readbyqxmd.com/read/28698576/multimodal-imaging-quality-control-of-epithelia-regenerated-with-cultured-human-donor-corneal-limbal-epithelial-stem-cells
#10
Marco Lombardo, Sebastiano Serrao, Vanessa Barbaro, Enzo Di Iorio, Giuseppe Lombardo
Current imaging techniques for the characterization of differentiated corneal limbal stem cells are destructive and cannot be used in eye bank for monitoring the regenerated epithelium in culture. We presented a minimally invasive, multimodal, marker-free imaging method for the investigation of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells. Two-photon fluorescence and harmonic generation signals were collected from specimens in culture and used for evaluating the structure and morphology of epithelia cultured on two different bio-scaffolds; in addition, donor human corneal tissues were used as controls...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28697743/integrative-analysis-of-copy-number-and-gene-expression-in-breast-cancer-using-formalin-fixed-paraffin-embedded-core-biopsy-tissue-a-feasibility-study
#11
Mahesh Iddawela, Oscar Rueda, Jenny Eremin, Oleg Eremin, Jed Cowley, Helena M Earl, Carlos Caldas
BACKGROUND: An absence of reliable molecular markers has hampered individualised breast cancer treatments, and a major limitation for translational research is the lack of fresh tissue. There are, however, abundant banks of formalin-fixed paraffin-embedded (FFPE) tissue. This study evaluated two platforms available for the analysis of DNA copy number and gene expression using FFPE samples. METHODS: The cDNA-mediated annealing, selection, extension, and ligation assay (DASL™) has been developed for gene expression analysis and the Molecular Inversion Probes assay (Oncoscan™), were used for copy number analysis using FFPE tissues...
July 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28697461/direct-induction-of-functional-neuronal-cells-from-fibroblast-like-cells-derived-from-adult-human-retina
#12
Lili Hao, Zhen Xu, Hui Sun, Wu Luo, Youchen Yan, Jing Wang, Jingyi Guo, Yizhi Liu, Shuyi Chen
Obtaining and manipulating neuronal cells are critical for neural biology basic mechanism studies and translational applications. Recent advances in protocol development and mechanism dissections have made direct induction of neuronal cells from other somatic cells (iN) a promising strategy for such purposes. In this study, we established a protocol to expand a population of fibroblast-like cells from adult human retinal tissues, which can be reprogrammed into iNs by forced expression of neurogenic transcription factors...
June 29, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28696436/reduced-trpc6-mrna-levels-in-the-blood-cells-of-patients-with-alzheimer-s-disease-and-mild-cognitive-impairment
#13
R Lu, J Wang, R Tao, J Wang, T Zhu, W Guo, Y Sun, H Li, Y Gao, W Zhang, C J Fowler, Q Li, S Chen, Z Wu, C L Masters, C Zhong, N Jing, Y Wang, Y Wang
Transient receptor potential canonical 6 (TRPC6) inhibits β-amyloid (Aβ) production. Hyperforin, the TRPC6 agonist, reduces Aβ levels and improves cognitive performance in Alzheimer's disease (AD) models. However, it's unknown whether TRPC6 expression is changed in AD patients. In this case-control study, we measured TRPC6 expression levels in the peripheral blood cells of four independent AD sets from five hospitals and one mild cognitive impairment (MCI) set from a local community (229 AD, 70 MCI, 40 Parkinson disease and 359 controls from China, total n=698) using quantitative real-time PCR assay...
July 11, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28696355/abcc6-and-pseudoxanthoma-elasticum-the-face-of-a-rare-disease-from-genetics-to-advocacy
#14
REVIEW
Karobi Moitra, Sonia Garcia, Michelle Jaldin, Clementine Etoundi, Donna Cooper, Anna Roland, Patrice Dixon, Sandra Reyes, Sevilay Turan, Sharon Terry, Michael Dean
Pseudoxanthoma elasticum (PXE) is an autosomal recessive disorder characterized by the mineralization of connective tissues in the body. Primary manifestation of PXE occurs in the tissues of the skin, eyes, and cardiovascular system. PXE is primarily caused by mutations in the ABCC6 gene. The ABCC6 gene encodes the trans-membrane protein ABCC6, which is highly expressed in the kidneys and liver. PXE has high phenotypic variability, which may possibly be affected by several modifier genes. Disease advocacy organizations have had a pivotal role in bringing rare disease research to the forefront and in helping to sustain research funding for rare genetic diseases in order to help find a treatment for these diseases, pseudoxanthoma elasticum included...
July 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28693600/normal-breast-tissue-dna-methylation-differences-at-regulatory-elements-are-associated-with-the-cancer-risk-factor-age
#15
Kevin C Johnson, E Andres Houseman, Jessica E King, Brock C Christensen
BACKGROUND: The underlying biological mechanisms through which epidemiologically defined breast cancer risk factors contribute to disease risk remain poorly understood. Identification of the molecular changes associated with cancer risk factors in normal tissues may aid in determining the earliest events of carcinogenesis and informing cancer prevention strategies. METHODS: Here we investigated the impact cancer risk factors have on the normal breast epigenome by analyzing DNA methylation genome-wide (Infinium 450 K array) in cancer-free women from the Susan G...
July 10, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28692670/patient-reported-outcomes-and-survival-in-multiple-sclerosis-a-10-year-retrospective-cohort-study-using-the-multiple-sclerosis-impact-scale-29
#16
Joel Raffel, Alison Wallace, Djordje Gveric, Richard Reynolds, Tim Friede, Richard Nicholas
BACKGROUND: There is increasing emphasis on using patient-reported outcomes (PROs) to complement traditional clinical outcomes in medical research, including in multiple sclerosis (MS). Research, particularly in oncology and heart failure, has shown that PROs can be prognostic of hard clinical endpoints such as survival time (time from study entry until death). However, unlike in oncology or cardiology, it is unknown whether PROs are associated with survival time in neurological diseases...
July 2017: PLoS Medicine
https://www.readbyqxmd.com/read/28691499/eradication-of-hiv-from-tissue-reservoirs-challenges-for-the-cure
#17
Rebecca R Rose, David J Nolan, Ekaterina Maidji, Cheryl Stoddart, Elyse J Singer, Susanna Lamers, Michael McGrath
The persistence of Human Immunodeficiency Virus (HIV) infection, even after lengthy and successful combined anti-retroviral therapy (cART), has precluded an effective cure. The anatomical locations in which and biological mechanisms through which the viral population is maintained remain unknown. Much research has focused nearly exclusively on circulating resting T-cells as the predominant source of persistent HIV, a strategy with limited success in developing an effective cure strategy. Here, we review research supporting the importance of tissues and other immune cells for HIV maintenance and expansion, including the central nervous system, lymph nodes, and macrophages...
July 9, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28690730/septic-arthritis-of-the-pubis-symphysis-clinical-and-therapeutic-features
#18
Zeineb Alaya, Houneida Zaghouani, Walid Osman, Lassad Hassini, Nader Naouar, Mohamed Laziz Ben Ayèche, Elyès Bouajina
Septic arthritis of the pubis symphysis is rare and difficult to diagnose. The objective of our study was to describe the biological, clinical, radiological and therapeutic aspects of this disease. This is a retrospective study of 4 cases of septic arthritis of the pubic symphysis collected in the Department of Rheumatology and Orthopaedics in Sousse in Tunisia over a period of 16 years (2000-2016). Our population consists of 3 women and one men. The mean age was 47 years (18-83). Clinical signs of appeal were inflammatory groin pain, pubic pain and fever...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28680541/understanding-the-cellular-and-molecular-alterations-in-ptsd-brains-the-necessity-of-post-mortem-brain-tissue
#19
EDITORIAL
Geertje M de Lange
The personal, social and economic burden of post-traumatic stress disorder (PTSD) is high and therapeutic approaches are only partially effective. Therefore, there is an urgent need to understand the cellular and molecular alterations in PTSD brains in order to design more effective treatment strategies. Although brain imaging strategies have considerably improved our understanding of PTSD, these strategies cannot identify molecular and cellular changes. Post-mortem examination of the brain is a crucial strategy to advance our understanding of the underlying neuropathology, neurochemistry and molecular pathways of PTSD...
2017: European Journal of Psychotraumatology
https://www.readbyqxmd.com/read/28680538/a-tumor-specific-antibody-to-aid-breast-cancer-screening-in-women-with-dense-breast-tissue
#20
Lopamudra Das Roy, Lloye M Dillon, Ru Zhou, Laura J Moore, Chad Livasy, Joe M El-Khoury, Rahul Puri, Pinku Mukherjee
Screening for breast cancer has predominantly been done using mammography. Unfortunately, mammograms miss 50% cancers in women with dense breast tissue. Multi-modal screenings offer the best chance of enhancing breast cancer screening effectiveness. We evaluated the use of TAB004, an antibody that recognizes the tumor form of the glycoprotein MUC1 (tMUC1), to aid early detection of breast cancer. Our experimental approach was to follow tMUC1 from the tissue into circulation. We found that 95% of human breast cancer tissues across all subtypes stained positive for TAB004...
March 2017: Genes & Cancer
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