keyword
https://read.qxmd.com/read/38527285/support-for-research-biobanks-among-medical-students-in-the-republic-of-kazakhstan
#1
JOURNAL ARTICLE
Dariusz Walkowiak, Alua Miraleyeva, Tamara Batyrova, Jan Domaradzki
Background: Over the past few years, several biobanks have been organized in the Republic of Kazakhstan (RoK). As research biobanks are becoming an increasingly important tool for precision medicine, they require competent biobankers who will help disseminate the idea of biobanking throughout the society and support donation for research purposes. This study aimed to assess the support for research biobanks among medical students (MSs) in Kazakhstan. Methods: This research includes data from an anonymous, self-administered online questionnaire regarding MSs' support for research biobanks in the RoK...
March 25, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38527284/effect-of-moderate-static-magnetic-fields-on-mice-oocyte-vitrification-calcium-related-genes-expression
#2
JOURNAL ARTICLE
Sara Soleimani Pargoo, Farzaneh Baniasadi, Vida Sadat Kazemein Jasemi, Samira Hajiaghalou, Mohsen Gharanfoli, Rouhollah Fathi
The ability to cryopreserve oocytes without ultrastructural injury has been a concern in the development and use of methods to preserve female reproduction. The stability of the cell membrane must be preserved to reduce the damage caused by ice crystals during vitrification. One approach that has been explored is the use of static magnetic fields (SMFs), which are believed to influence cell membrane stability. In this study, the in vitro effects of SMF that range between 20-80 mT on the vitrification of mice germinal vesicle (GV) oocytes were studied...
March 25, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38526566/evaluation-of-collection-and-processing-conditions-for-gene-expression-analysis-using-human-myeloid-cells
#3
JOURNAL ARTICLE
Hitoshi Miyashita, Issey Takehara, Masatoshi Nishimura, Gensuke Takayama, Hiroyuki Sumi, Michinori Kadokura, Daisuke Nakai
Background: The population of blast cells among peripheral blood mononuclear cells (PBMCs) obtained from patients is a desirable specimen for analyzing gene expression in diseases including acute myeloid leukemia. Although the enrichment of blast cells often needs to be performed at a central laboratory, acceptable conditions for sample transport from clinical sites remain to be established. Methods: We evaluated storage temperature, duration, and tube type before initiating sample processing for the analysis of cluster of differentiation (CD)33+ myeloid cells among PBMCs as an alternative to CD34+ /CD33+ blast cells...
March 22, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38526565/catalase-and-uric-acid-prevent-morphological-damage-to-the-sperm-flagella-of-colossoma-macropomum-during-96-hours-at-low-storage-temperatures
#4
JOURNAL ARTICLE
Yugo M Pastrana, Jaydione L Marcon, Amanda P de Amaral, Francisco Bruno P Santos, Emerson S Lima, Leonard D R Acho, Rodrigo Otávio S de Souza, Carolina C Grando, Danilo P Streit Junior, Leandro Godoy
Oxidative stress is one of the main causes of loss of sperm function during chilled storage. The aim of the current study was to evaluate the effects of a fructose-based extender, which was supplemented with catalase or uric acid, on the motility, viability, morphological integrity, and lipid peroxidation (LPO) of Colossoma macropomum spermatozoa. Sperm was diluted in extenders containing catalase (0; 0.1; 0.8; and 1.5 kU/L) or uric acid (0; 0.25; 0.5; and 1.0 mmol/L) and then stored at 4.3 ± 0...
March 22, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38526134/nephropathia-epidemica-caused-by-puumala-virus-in-bank-voles-scania-southern-sweden
#5
JOURNAL ARTICLE
Jiaxin Ling, Elin Economou Lundeberg, Anishia Wasberg, Inês R Faria, Sanja Vucicevic, Bo Settergren, Åke Lundkvist
In 2018, a local case of nephropathia epidemica was reported in Scania, southern Sweden, more than 500 km south of the previously known presence of human hantavirus infections in Sweden. Another case emerged in the same area in 2020. To investigate the zoonotic origin of those cases, we trapped rodents in Ballingslöv, Norra Sandby, and Sörby in southern Sweden during 2020‒2021. We found Puumala virus (PUUV) in lung tissues from 9 of 74 Myodes glareolus bank voles by screening tissues using a hantavirus pan-large segment reverse transcription PCR...
April 2024: Emerging Infectious Diseases
https://read.qxmd.com/read/38521608/impact-of-fda-s-hct-p-zikv-recommendations-on-cord-blood-unit-eligibility-and-utilization-in-a-large-public-cord-blood-bank
#6
JOURNAL ARTICLE
Dana S Guggenheim, Joanne Kurtzberg, Beth H Shaz
BACKGROUND: Cord blood units (CBUs) that are ineligible for licensure due to incomplete compliance with FDA recommendations may be used for hematopoietic stem cell transplantation under urgent medical need and an Investigational Drug Application. The largest reason for CBU donor ineligibility is Zika virus (ZIKV) risk. The study's objective was to analyze the impact of current FDA recommendations for ZIKA risk on a large public cord blood bank and propose updated recommendations. METHODS: We performed a retrospective analysis of Carolinas Cord Blood Bank (CCBB), an FDA licensed public CBB, using data from January 1, 2016 to November 21, 2023 and compared FDA recommendations for transfusion transmitted infections (TTI) for blood products and relevant communicable disease agents or diseases for human cell, tissue, or cellular or tissue-based products (HCT/Ps)...
March 23, 2024: Stem Cells Translational Medicine
https://read.qxmd.com/read/38517441/intraoperative-evaluation-of-breast-tissues-during-breast-cancer-operations-using-the-masspec-pen
#7
JOURNAL ARTICLE
Kyana Y Garza, Mary E King, Chandandeep Nagi, Rachel J DeHoog, Jialing Zhang, Marta Sans, Anna Krieger, Clara L Feider, Alena V Bensussan, Michael F Keating, John Q Lin, Min Woo Sun, Robert Tibshirani, Christopher Pirko, Kirtan A Brahmbhatt, Ahmed R Al-Fartosi, Alastair M Thompson, Elizabeth Bonefas, James Suliburk, Stacey A Carter, Livia S Eberlin
IMPORTANCE: Surgery with complete tumor resection remains the main treatment option for patients with breast cancer. Yet, current technologies are limited in providing accurate assessment of breast tissue in vivo, warranting development of new technologies for surgical guidance. OBJECTIVE: To evaluate the performance of the MasSpec Pen for accurate intraoperative assessment of breast tissues and surgical margins based on metabolic and lipid information. DESIGN, SETTING, AND PARTICIPANTS: In this diagnostic study conducted between February 23, 2017, and August 19, 2021, the mass spectrometry-based device was used to analyze healthy breast and invasive ductal carcinoma (IDC) banked tissue samples from adult patients undergoing breast surgery for ductal carcinomas or nonmalignant conditions...
March 4, 2024: JAMA Network Open
https://read.qxmd.com/read/38513716/validation-of-preloaded-dmek-donor-tissues-a-laboratory-based-study-on-endothelial-cell-viability-and-comparison-of-two-f-mark-inks
#8
JOURNAL ARTICLE
Narisa Dhupar, Sara AlShaker, Jenny Chai, Judy Yan, Ness Little, Christine Humphreys, Clara C Chan, Stephan Ong Tone
OBJECTIVE: To investigate endothelial cell loss (ECL) associated with Descemet membrane endothelial keratoplasty (DMEK) donor tissues preloaded in the DMEK RAPID transport system after 1 and 5 days and to compare prestamping with 2 different F-mark inks. METHODS: DMEK donor tissues were stripped, marked with gentian violet dye applied as an F-mark, trephined, stained with trypan blue, and then preloaded into the DMEK RAPID transport system by an eye bank technician...
March 19, 2024: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://read.qxmd.com/read/38506708/dietary-fiber-is-a-critical-determinant-of-pathologic-ilc2-responses-and-intestinal-inflammation
#9
JOURNAL ARTICLE
Mohammad Arifuzzaman, Tae Hyung Won, Hiroshi Yano, Jazib Uddin, Elizabeth R Emanuel, Elin Hu, Wen Zhang, Ting-Ting Li, Wen-Bing Jin, Alex Grier, Sanchita Kashyap, Chun-Jun Guo, Frank C Schroeder, David Artis
Innate lymphoid cells (ILCs) can promote host defense, chronic inflammation, or tissue protection and are regulated by cytokines and neuropeptides. However, their regulation by diet and microbiota-derived signals remains unclear. We show that an inulin fiber diet promotes Tph1-expressing inflammatory ILC2s (ILC2INFLAM) in the colon, which produce IL-5 but not tissue-protective amphiregulin (AREG), resulting in the accumulation of eosinophils. This exacerbates inflammation in a murine model of intestinal damage and inflammation in an ILC2- and eosinophil-dependent manner...
May 6, 2024: Journal of Experimental Medicine
https://read.qxmd.com/read/38506125/brain-banking-in-the-united-states-and-europe-importance-challenges-and-future-trends
#10
JOURNAL ARTICLE
Benjamin Danner, Angelique D Gonzalez, William Cole Corbett, Mohammad Alhneif, Shahroo Etemadmoghadam, Julie Parker-Garza, Margaret E Flanagan
In recent years, brain banks have become valuable resources for examining the molecular underpinnings of various neurological and psychological disorders including Alzheimer disease and Parkinson disease. However, the availability of brain tissue has significantly declined. Proper collection, preparation, and preservation of postmortem autopsy tissue are essential for optimal downstream brain tissue distribution and experimentation. Collaborations between brain banks through larger networks such as NeuroBioBank with centralized sample request mechanisms promote tissue distribution where brain donations are disproportionately lower...
March 20, 2024: Journal of Neuropathology and Experimental Neurology
https://read.qxmd.com/read/38502609/thermopcd-a-database-of-molecular-dynamics-trajectories-of-antibody-antigen-complexes-at-physiologic-and-fever-range-temperatures
#11
JOURNAL ARTICLE
Puneet K Singh, Razvan C Stan
Progression of various cancers and autoimmune diseases is associated with changes in systemic or local tissue temperatures, which may impact current therapies. The role of fever and acute inflammation-range temperatures on the stability and activity of antibodies relevant for cancers and autoimmunity is unknown. To produce molecular dynamics (MD) trajectories of immune complexes at relevant temperatures, we used the Research Collaboratory for Structural Bioinformatics (RCSB) database to identify 50 antibody:antigen complexes of interest, in addition to single antibodies and antigens, and deployed Groningen Machine for Chemical Simulations (GROMACS) to prepare and run the structures at different temperatures for 100-500 ns, in single or multiple random seeds...
March 19, 2024: Database: the Journal of Biological Databases and Curation
https://read.qxmd.com/read/38501564/determinants-of-left-atrial-local-impedance-relationships-with-contact-force-atrial-fibrosis-and-rhythm
#12
JOURNAL ARTICLE
Alexander P Bates, John Paisey, Arthur Yue, Phil Banks, Paul R Roberts, Waqas Ullah
INTRODUCTION: The relationships between baseline tissue local impedance (LI), contact force (CF), atrial fibrosis, and atrial rhythm are uninvestigated in a clinical setting. We compared the relationship of LI and CF between atrial fibrillation (AF) and sinus rhythm (SR) accounting for the effects of atrial fibrosis as assessed by bipolar voltage and LI. METHODS: Patients undergoing persistent AF ablation were recruited. LI was recorded referenced to patient blood pool (LIr) and concurrent to changes in CF, with data collected at the same locations in AF and SR...
March 19, 2024: Journal of Cardiovascular Electrophysiology
https://read.qxmd.com/read/38497765/update-of-the-minimum-information-about-biobank-data-sharing-miabis-core-terminology-to-the-3-rd-version
#13
JOURNAL ARTICLE
Niina Eklund, Cäcilia Engels, Michael Neumann, Andrzej Strug, Esther van Enckevort, Ronny Baber, Margreet Bloemers, Annelies Debucquoy, Aad van der Lugt, Heimo Müller, Lauri Parkkonen, Philip R Quinlan, Esmond Urwin, Petr Holub, Kaisa Silander, Gabriele Anton
Introduction: The Minimum Information About BIobank Data Sharing (MIABIS) is a biobank-specific terminology enabling the sharing of biobank-related data for different purposes across a wide range of database implementations. After 4 years in use and with the first version of the individual-level MIABIS component Sample , Sample donor, and Event , it was necessary to revise the terminology, especially to include biobanks that work more in the data domain than with samples. Materials & Methods: Nine use-cases representing different types of biobanks, studies, and networks participated in the development work...
March 18, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38497547/technology-for-biobanking-of-epididymal-spermatozoa-from-patient-with-obstructive-azoospermia-case-report-about-baby-born-after-conventional-freezing-only-with-a-nonpermeable-cryoprotectant-360-kda-polyvinylpyrrolidone
#14
JOURNAL ARTICLE
Maryna Petrushko, Taisiia Yurchuk, Volodymyr Piniaiev, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Volodimir Isachenko
This publication reports, for the first time, the birth of a healthy child after intracytoplasmic sperm injection (ICSI) of motile spermatozoa after conventional ("slow") freezing of epididymal spermatozoa using 5% polyvinylpyrrolidone (PVP) of high molecular weight (360 kDa). Cryopreservation solution with 10% PVP was added to 30 µL of spermatozoa suspension in a 1:1 ratio, with a final PVP concentration of 5%. Then, polycarbonate capillaries for oocyte denudation with a diameter of 170 µm were filled with 60 µL of the resulting sperm suspension...
March 18, 2024: Biopreservation and Biobanking
https://read.qxmd.com/read/38496007/acceleration-of-benzo-a-pyrene-induced-colon-carcinogenesis-by-western-diet-in-a-rat-model-of-colon-cancer
#15
JOURNAL ARTICLE
Kelly L Harris, Kenneth J Harris, Leah D Banks, Samuel E Adunyah, Aramandla Ramesh
Colorectal cancer (CRC) is the third leading cause of cancer-related mortalities in the USA and around 52,550 people were expected to die from this disease by December 2023. The objective of this study was to investigate the effect of diet type on benzo(a)pyrene [B(a)P]-induced colon cancer in an adult male rat model, the Polyposis In the Rat Colon (PIRC) kindred type. Groups of PIRC rats (n = 10) were fed with AIN-76A regular diet (RD) or Western diet (WD) and received 25, 50 and 100 µg B(a)P/kg body wt...
2024: Current research in toxicology
https://read.qxmd.com/read/38495675/influence-of-donor-factors-on-descemet-membrane-endothelial-keratoplasty-dmek-graft-preparation-outcome
#16
JOURNAL ARTICLE
Silvia Schrittenlocher, Jithmi Weliwitage, Mario Matthaei, Björn Bachmann, Claus Cursiefen
PURPOSE: To determine which donor characteristics, like previous diseases and surgeries, influence the severity of the DM/endothelial lamella preparation prior to DMEK-surgery. PATIENTS AND METHODS: Retrospective cross-sectional single-center study is presented. Eight hundred and forty-six eyes with DMEK-surgery between 01/2018 and 01/2021 performed at the University Hospital Cologne, Germany, were included. Information regarding the donors' previous diseases and surgeries were provided by a large database of a cornea bank (Multi Tissue Bank Mecklenburg-Vorpommern) and merged with the Cologne DMEK database, which contains information regarding preparation characteristics of the surgeon-prepared graft directly preoperatively...
2024: Clinical Ophthalmology
https://read.qxmd.com/read/38495506/role-of-epstein-barr-virus-in-breast-cancer-correlation-with-clinical-outcome-and-survival-analysis
#17
JOURNAL ARTICLE
Yi-Chiung Hsu, Ming-Han Tsai, Guani Wu, Chien-Liang Liu, Yuan-Ching Chang, Hung-Bun Lam, Pei-Yu Su, Chun-Fan Lung, Po-Sheng Yang
Background: Breast cancer is the most prevalent cancer among women worldwide. The potential involvement of Epstein-Barr virus (EBV) in breast cancer pathogenesis has been a subject of debate, but its correlation with clinical outcomes remains uncertain. Methods: In this study, we collected 276 pathologically confirmed breast cancer tissue samples from the tissue bank of MacKay Memorial Hospital and the National Health Research Institutes in Taiwan. DNA was extracted from frozen tissue using The QIAamp DNA Mini Kit...
2024: Journal of Cancer
https://read.qxmd.com/read/38494076/bendamustine-as-lymphodepletion-for-brexucabtagene-autoleucel-therapy-of-mantle-cell-lymphoma
#18
JOURNAL ARTICLE
Elise A Chong, Emeline R Chong, Dylan Therwhangher, Sunita D Nasta, Daniel J Landsburg, Stefan K Barta, Jakub Svoboda, James N Gerson, Guido Ghilardi, Luca Paruzzo, Joseph A Fraietta, Elizabeth Weber, Natalie Stefano, David L Porter, Noelle V Frey, Alfred L Garfall, Marco Ruella, Stephen J Schuster
BACKGROUND: Brexucabtagene autoleucel (brexu-cel) is an autologous CD19-directed chimeric antigen receptor (CAR) T-cell therapy approved for treatment of relapsed/refractory mantle cell lymphoma (MCL). During a fludarabine shortage, we used bendamustine as an alternative to standard cyclophosphamide/fludarabine (cy/flu) lymphodepletion (LD) prior to brexu-cel. OBJECTIVES: We assessed MCL patient outcomes as well as CAR T-cell expansion and persistence after brexu-cel following bendamustine or cy/flu LD at our center...
March 15, 2024: Transplantation and cellular therapy
https://read.qxmd.com/read/38490707/presentation-and-multidisciplinary-management-of-a-unique-case-of-lower-limb-dysmelia-resulting-from-amniotic-band-syndrome
#19
JOURNAL ARTICLE
Katherine Radcliffe, Kajal Gohil, James D Bedford
A neonate was born with a unique congenital lower limb dysmelia due to an abnormal presentation of amniotic band syndrome. An anomalous soft tissue tether from the plantar surface of the right foot to the right buttock caused extreme knee flexion, tibial rotation and malformation of the developing foot. This complex malformation required a multidisciplinary team (MDT) approach to decide between reconstruction and amputation. The band of tissue was released operatively at 73 days postdelivery, improving knee extension, and the tissue was banked on the thigh as a tube pedicle for future reconstruction...
March 15, 2024: BMJ Case Reports
https://read.qxmd.com/read/38489016/variability-of-bmp-2-content-in-dbm-products-derived-from-different-long-bone
#20
JOURNAL ARTICLE
Yong-Jie Zhao, Gang Yin, Bin Liu, Xiao-Qiang Deng, Hai-Yan Cao, Ying Liu
Demineralized bone matrix (DBM) has been regarded as an ideal bone substitute as a native carrier of bone morphogenetic proteins (BMPs) and other growth factors. However, the osteoinductive properties diverse in different DBM products. We speculate that the harvest origin further contributing to variability of BMPs contents in DBM products besides the process technology. In the study, the cortical bone of femur, tibia, humerus, and ulna from a signal donor were prepared and followed demineralizd into DBM products...
March 15, 2024: Cell and Tissue Banking
keyword
keyword
10242
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.