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https://www.readbyqxmd.com/read/28527016/chemical-derivatization-of-metabolite-mass-profiling-of-the-recretohalophyte-aeluropus-lagopoides-revealing-salt-stress-tolerance-mechanism
#1
Murali Krishna Paidi, Parinita Agarwal, Prashant More, Pradeep K Agarwal
Plants are the primary producers of food for human being. Their intracellular environment alternation is influenced by abiotic stress factors such as drought, heat and soil salinity. Aeluropus lagopoides is a strong halophyte that grows with ease under high saline muddy banks of creeks of Gujarat, India. To study the response of salinity on metabolite changes in Aeluropus, three treatments, i.e. control, salinity and recovery, were selected for both shoot and root tissue. The cytosolic metabolite state was analysed by molecular chemical derivatization gas chromatography mass profiling...
May 19, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28523495/fresh-frozen-versus-formalin-fixed-paraffin-embedded-for-mass-spectrometry-imaging
#2
Ekta Patel
Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28515451/hoxa7-hoxa9-and-hoxa10-are-differentially-expressed-in-clival-and-sacral-chordomas
#3
Daniela Jäger, Thomas F E Barth, Silke Brüderlein, Angelika Scheuerle, Beate Rinner, Adrian von Witzleben, André Lechel, Patrick Meyer, Regine Mayer-Steinacker, Alexandra von Baer, Markus Schultheiss, Christian R Wirtz, Peter Möller, Kevin Mellert
Chordomas are rare tumours of the bone arising along the spine from clivus to sacrum. We compared three chordoma cell lines of the clivus region including the newly established clivus chordoma cell line, U-CH14, with nine chordoma cell lines originating from sacral primaries by morphology, on genomic and expression levels and with patient samples from our chordoma tissue bank. Clinically, chordomas of the clivus were generally smaller in size at presentation and patients with sacral chordomas had more metastases and more often recurrent disease...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28512306/structure-based-docking-studies-towards-exploring-potential-anti-androgen-activity-of-selected-phytochemicals-against-prostate-cancer
#4
Anshika N Singh, Meghna M Baruah, Neeti Sharma
Prostate cancer (PCa) is the second most common malignancy amongst men worldwide. Under PCa maintenance therapy drugs acting as antagonists/partial agonists of hormone receptors against the prostate tissue are used in clinical practices. Prominent drugs being Cyproterone acetate, Flutamide, Bicalutamide, they not only cause acute and long-term toxicity, but also develops drug resistance among patients. Our focus has been on phytochemicals which do not exhibit any cytotoxicity and have significant androgen receptor (AR) inhibition activity...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28509388/proteomic-explorations-of-autism-spectrum-disorder
#5
Nicholas Szoko, Adam J McShane, Marvin R Natowicz
Proteomics, the large-scale study of protein expression in cells and tissues, is a powerful tool to study the biology of clinical conditions and has provided significant insights in many experimental systems. Herein, we review the basics of proteomic methodology and discuss challenges in using proteomic approaches to study autism. Unlike other experimental approaches, such as genomic approaches, there have been few large-scale studies of proteins in tissues from persons with autism. Most of the proteomic studies on autism used blood or other peripheral tissues; few studies used brain tissue...
May 16, 2017: Autism Research: Official Journal of the International Society for Autism Research
https://www.readbyqxmd.com/read/28495906/emergency-corneal-grafting-in-the-uk-a-6-year-analysis-of-the-uk-transplant-registry
#6
Parwez Hossain, Abdo Karim Tourkmani, Dimitri Kazakos, Mark Jones, David Anderson
BACKGROUND: Corneal graft (CG) surgery is the most common and successful tissue transplant worldwide. A small and important group of patients are operated in emergency situations, typically to save a perforated eye. Our knowledge of the indications and outcomes of emergency corneal graft (eCG) is limited. METHODS: Retrospective, multifactorial analysis of all CGs registered by the UK Transplant Service from April 1999 to March 2005. RESULTS: A total of 12 976 CGs were performed...
May 11, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28492743/presence-of-cells-in-fresh-frozen-allogeneic-bone-grafts-from-different-tissue-banks
#7
Libério França Coutinho, Juliano Batista do Amaral, Érico Brito Dos Santos, Elizabeth Ferreira Martinez, Victor Angelo M Montalli, José Luiz Cintra Junqueira, Vera Cavalcanti de Araújo, Marcelo Henrique Napimoga
Bone replacement materials have been widely used to reconstruct atrophic jawbones. Based on previous reports demonstrating the presence of viable cells in bone blocks even after processing by musculoskeletal tissue banks for orthopedic use, the aim of this study was to evaluate the presence of cells in bone blocks from three Brazilian tissue banks for maxillary reconstructions. All samples were processed by the respective tissue banks, according to the guidelines of the Brazilian National Sanitary Surveillance Agency...
March 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/28489792/eye-bank-prepared-versus-surgeon-cut-endothelial-graft-tissue-for-descemet-membrane-endothelial-keratoplasty-an-observational-study
#8
Marie Regnier, Céline Auxenfans, Delphine Maucort-Boulch, Anne-Sophie Marty, Odile Damour, Carole Burillon, Viridiana Kocaba
The purpose of this article is to examine outcomes of Descemet membrane endothelial keratoplasty (DMEK) performed with cornea bank (CB) prestripped tissue and surgeon stripped tissue (SST).This retrospective study examined subjects who underwent DMEK with CB or surgeon prepared tissue for Fuchs endothelial corneal dystrophy. Best-corrected visual acuity (BCVA), corneal thickness, endothelial cell count (ECC), and complications were examined before and throughout a 6-month postoperative period.Eleven CB and 22 SST subjects were included...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28489577/pancreatic-cancer-ascites-xenograft-an-expeditious-model-mirroring-advanced-therapeutic-resistant-disease
#9
Talia Golan, Chani Stossel, Michael Schvimer, Dikla Atias, Sharon Halperin, Ella Buzhor, Maria Raitses-Gurevich, Keren Cohen, Sara Pri-Chen, Julie Wilson, Robert E Denroche, Ilinca Lungu, John M S Bartlett, Faridah Mbabaali, Yosef Yarden, Nishanth Belugali Nataraj, Steven Gallinger, Raanan Berger
Pancreatic ductal adenocarcinoma has limited treatment options. There is an urgent need for developing appropriate pre-clinical models recapitulating metastatic disease, the most common clinical scenario at presentation. Ascites accumulation occurs in up to 20-30% of patients with pancreatic cancer; this milieu represents a highly cellular research resource of metastatic peritoneal spread. In this study, we utilized pancreatic ascites/pleural effusion cancer cells to establish patient derived xenografts.Ascites/pleural effusion-patient derived xenografts were established from twelve independent cases...
April 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/28488967/purinergic-control-of-red-blood-cell-metabolism-novel-strategies-to-improve-red-cell-storage-quality
#10
REVIEW
Kaiqi Sun, Angelo D'alessandro, Yang Xia
Transfusion of stored blood is regarded as one of the great advances in modern medicine. However, during storage in the blood bank, red blood cells (RBCs) undergo a series of biochemical and biomechanical changes that affect cell morphology and physiology and potentially impair transfusion safety and efficacy. Despite reassuring evidence from clinical trials, it is universally accepted that the storage lesion(s) results in the altered physiology of long-stored RBCs and helps explain the rapid clearance of up to one-fourth of long-stored RBCs from the recipient's bloodstream at 24 hours after administration...
April 12, 2017: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/28472471/successful-immunosuppressant-free-heterotopic-transplantation-of-tracheal-allografts-in-the-pig
#11
Julien De Wolf, Mathias Brieu, Christophe Zawadzki, Alexandre Ung, Eric Kipnis, Ramadan Jashari, Thomas Hubert, Pierre Fayoux, Christophe Mariette, Marie-Christine Copin, Alain Wurtz
OBJECTIVES: It has been demonstrated that both heterotopic and orthotopic transplants of epithelium-denuded cryopreserved tracheal allografts are feasible in immunosuppressant-free rabbits. Validation of these results in large animals is required before considering clinical applications. We evaluated the viability, immune tolerance and strain properties of such tracheal allografts heterotopically transplanted in a pig model. METHODS: Ten tracheal segments, 5 short (5 rings) and 5 long (10 rings), were obtained from male Landrace pigs...
May 2, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28469991/donor-transmitted-melanoma-after-limbal-stem-cell-transplantation
#12
Anas Alsara, Muhammad Rafi
Limbal Stem Cells are a unique cell line located at the corneal limbus. They are responsible for regenerating and restoring corneal epithelial layers. Limbal stem cell transplantation is a promising technique that has been used to treat several hereditary and acquired corneal diseases. Cornea tissue lack vascularity. Hence, there were no special restrictions on collecting ocular tissues from donors with a diagnosis of metastatic melanoma. We are reporting a case of a patient who developed an ocular melanoma after she had limbal stem cell transplantation from a donor with history of melanoma...
April 2017: Avicenna Journal of Medicine
https://www.readbyqxmd.com/read/28467235/applying-the-health-belief-model-and-an-integrated-behavioral-model-to-promote-breast-tissue-donation-among-asian-americans
#13
Autumn Shafer, Kelly Kaufhold, Yunjuan Luo
An important part in the effort to prevent, treat, and cure breast cancer is research done with healthy breast tissue. The Susan G. Komen for the Cure Tissue Bank at Indiana University Simon Cancer Center (KTB) encourages women to donate a small amount of healthy breast tissue and then provides that tissue to researchers studying breast cancer. Although KTB has a large donor base, the volume of tissue samples from Asian women is low despite prior marketing efforts to encourage donation among this population...
May 3, 2017: Health Communication
https://www.readbyqxmd.com/read/28450146/alternatives-to-eye-bank-native-tissue-for-corneal-stromal-replacement
#14
REVIEW
Isabelle Brunette, Cynthia J Roberts, François Vidal, Mona Harissi-Dagher, Jean Lachaine, Heather Sheardown, Georges M Durr, Stéphanie Proulx, May Griffith
Corneal blindness is a major cause of blindness in the world and corneal transplantation is the only widely accepted treatment to restore sight in these eyes. However, it is becoming increasingly difficult for eye banks to meet the increasing demand for transplantable tissue, which is in part due to population aging. Donor tissue shortage is therefore a growing concern globally and there is a need for alternatives to human donor corneas. Biosynthetic corneal substitutes offer several significant advantages over native corneas: Large-scale production offers a powerful potential solution to the severe shortage of human donor corneas worldwide; Good manufacturing practices ensure sterility and quality control; Acellular corneal substitutes circumvent immune rejection induced by allogeneic cells; Optical and biomechanical properties of the implants can be adapted to the clinical need; and finally these corneal substitutes could benefit from new advances in biomaterials science, such as surface coating, functionalization and nanoparticles...
April 24, 2017: Progress in Retinal and Eye Research
https://www.readbyqxmd.com/read/28439732/skin-allograft-procurement-and-transplantation-in-mashhad-iran-are-burn-patients-needs-being-met
#15
Seyed Hassan Tavousi, Ali Ahmadabadi, Alireza Sedaghat, Ebrahim Khaleghi, Maryam Rashchi, Zohreh Bonakdaran
Only a single donor's tissue may save or improve lives of one hundred patients. Unfortunately, low governmental and media support of tissue procurement and transplantation programs is a worldwide problem. Loss of an effective tissue procurement program in many countries like Iran, may lead to loss of many thousands valuable tissues each year. To evaluate the rate of skin donation in Mashhad in comparison to other organs and tissues, we extracted the data related to tissue and organ procurement in Mashhad from 2001...
April 24, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28438482/manufacture-and-preparation-of-human-placenta-derived-mesenchymal-stromal-cells-for-local-tissue-delivery
#16
Lee Lankford, Y Julia Chen, Zoe Saenz, Priyadarsini Kumar, Connor Long, Diana Farmer, Aijun Wang
BACKGROUND: In this study we describe the development of a Current Good Manufacturing Practice (CGMP)-compliant process to isolate, expand and bank placenta-derived mesenchymal stromal cells (PMSCs) for use as stem cell therapy. We characterize the viability, proliferation and neuroprotective secretory profile of PMSCs seeded on clinical-grade porcine small intestine submucosa extracellular matrix (SIS-ECM; Cook Biotech). METHODS: PMSCs were isolated from early gestation placenta chorionic villus tissue via explant culture...
April 21, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28427436/a-3d-in-vitro-model-of-the-human-breast-duct-a-method-to-unravel-myoepithelial-luminal-interactions-in-the-progression-of-breast-cancer
#17
Edward P Carter, James A Gopsill, Jennifer J Gomm, J Louise Jones, Richard P Grose
BACKGROUND: 3D modelling fulfils a critical role in research, allowing for complex cell behaviour and interactions to be studied in physiomimetic conditions. With tissue banks becoming established for a number of cancers, researchers now have access to primary patient cells, providing the perfect building blocks to recreate and interrogate intricate cellular systems in the laboratory. The ducts of the human breast are composed of an inner layer of luminal cells supported by an outer layer of myoepithelial cells...
April 21, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28424723/evidence-mechanism-and-alternative-chemical-seedbank-level-control-of-glyphosate-resistance-of-a-rigid-ryegrass-lolium-rigidum-biotype-from-southern-spain
#18
Pablo T Fernández-Moreno, Fernando Bastida, Rafael De Prado
Rigid ryegrass (Lolium rigidum) is one of the most troublesome weeds in different crops in the Mediterranean region. A rigid ryegrass biotype from an olive grove in Jaén province (Andalusía, southern Spain), potentially resistant to glyphosate (RG), was tested for its resistance level through dose-response assays using a susceptible biotype (SG). To test the hypothesis of a non-target-site-based resistance, as point mutations are far less common mechanisms of glyphosate resistance, studies were also conducted to elucidate whether resistance was associated with biochemical, metabolism, molecular and/or physiological mechanisms...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28422434/rat-hindlimb-cryopreservation-and-transplantation-a-step-towards-organ-banking
#19
Amir Arav, Or Friedman, Yehudit Natan, Eyal Gur, Nir Shani
In 2016 over 5 million reconstructive procedures were performed in the USA. The recent successes of clinical vascularized composite allotransplantations (VCA), hand and face transplantations included, established the tremendous potential of these life-enhancing reconstructions. Nevertheless, Due to limited availability and lifelong immunosuppression, application is limited. Long term banking of composite transplants may increase the availability of esthetically compatible parts with partial or complete HLA matching, reducing the risk of rejection and the immunosuppressive burden...
April 19, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28421181/mct1-in-invasive-ductal-carcinoma-monocarboxylate-metabolism-and-aggressive-breast-cancer
#20
Jennifer M Johnson, Paolo Cotzia, Roberto Fratamico, Lekha Mikkilineni, Jason Chen, Daniele Colombo, Mehri Mollaee, Diana Whitaker-Menezes, Marina Domingo-Vidal, Zhao Lin, Tingting Zhan, Madalina Tuluc, Juan Palazzo, Ruth C Birbe, Ubaldo E Martinez-Outschoorn
Introduction: Monocarboxylate transporter 1 (MCT1) is an importer of monocarboxylates such as lactate and pyruvate and a marker of mitochondrial metabolism. MCT1 is highly expressed in a subgroup of cancer cells to allow for catabolite uptake from the tumor microenvironment to support mitochondrial metabolism. We studied the protein expression of MCT1 in a broad group of breast invasive ductal carcinoma specimens to determine its association with breast cancer subtypes and outcomes. Methods: MCT1 expression was evaluated by immunohistochemistry on tissue micro-arrays (TMA) obtained through our tumor bank...
2017: Frontiers in Cell and Developmental Biology
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