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https://www.readbyqxmd.com/read/28238108/enhanced-vascular-biocompatibility-of-decellularized-xeno-allogeneic-matrices-in-a-rodent-model
#1
M van Steenberghe, T Schubert, Y Guiot, C Bouzin, X Bollen, P Gianello
Glutaraldehyde preservation is the gold standard for cardiovascular biological prosthesis. However, secondary calcifications and the absence of tissue growth remain major limitations. Our study assessed in vitro and in vivo the biocompatibility of human (fascia lata, pericardium) and porcine tissues (pericardium, peritoneum) treated with a physicochemical procedure for decellularization and non-conventional pathogens inactivation. Biopsies were performed before and after treatment to assess decellularization (HE/Dapi staining/DNA quantification/MHC I/alpha gal immunostaining) and mechanical integrity...
February 25, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28227347/an-eemd-pca-approach-to-extract-heart-rate-respiratory-rate-and-respiratory-activity-from-ppg-signal
#2
Mohammod Abdul Motin, Chandan Kumar Karmakar, Marimuthu Palaniswami, Mohammod Abdul Motin, Chandan Kumar Karmakar, Marimuthu Palaniswami, Marimuthu Palaniswami, Mohammod Abdul Motin, Chandan Kumar Karmakar
The pulse oximeter's photoplethysmographic (PPG) signals, measure the local variations of blood volume in tissues, reflecting the peripheral pulse modulated by cardiac activity, respiration and other physiological effects. Therefore, PPG can be used to extract the vital cardiorespiratory signals like heart rate (HR), respiratory rate (RR) and respiratory activity (RA) and this will reduce the number of sensors connected to the patient's body for recording vital signs. In this paper, we propose an algorithm based on ensemble empirical mode decomposition with principal component analysis (EEMD-PCA) as a novel approach to estimate HR, RR and RA simultaneously from PPG signal...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225797/use-of-bovine-recombinant-prion-protein-and-real-time-quaking-induced-conversion-to-detect-cattle-transmissible-mink-encephalopathy-prions-and-discriminate-classical-and-atypical-l-and-h-type-bovine-spongiform-encephalopathy
#3
Soyoun Hwang, Justin J Greenlee, Eric M Nicholson
Prions are amyloid-forming proteins that cause transmissible spongiform encephalopathies through a process involving conversion from the normal cellular prion protein to the pathogenic misfolded conformation (PrPSc). This conversion has been used for in vitro assays including serial protein misfolding amplification and real-time quaking induced conversion (RT-QuIC). RT-QuIC can be used for the detection of prions in a variety of biological tissues from humans and animals. Extensive work has been done to demonstrate that RT-QuIC is a rapid, specific, and highly sensitive prion detection assay...
2017: PloS One
https://www.readbyqxmd.com/read/28220575/generation-of-storable-retinal-organoids-and-retinal-pigmented-epithelium-from-adherent-human-ips-cells-in-xeno-free-and-feeder-free-conditions
#4
Sacha Reichman, Amélie Slembrouck, Giuliana Gagliardi, Antoine Chaffiol, Angélique Terray, Céline Nanteau, Anais Potey, Morgane Belle, Oriane Rabesandratana, Jens Duebel, Gael Orieux, Emeline F Nandrot, José-Alain Sahel, Olivier Goureau
Human induced pluripotent stem cells (hiPSCs) are potentially useful in regenerative therapies for retinal disease. For medical applications, therapeutic retinal cells, such as retinal pigmented epithelial (RPE) cells or photoreceptor precursors, must be generated under completely defined conditions. To this purpose, we have developed a two-step xeno-free/feeder-free (XF/FF) culture system to efficiently differentiate hiPSCs into retinal cells. This simple method, relies only on adherent hiPSCs cultured in chemically defined media, bypassing embryoid body formation...
February 20, 2017: Stem Cells
https://www.readbyqxmd.com/read/28214960/axonal-disruption-in-white-matter-underlying-cortical-sulcus-tau-pathology-in-chronic-traumatic-encephalopathy
#5
Laurena Holleran, Joong Hee Kim, Mihika Gangolli, Thor Stein, Victor Alvarez, Ann McKee, David L Brody
Chronic traumatic encephalopathy (CTE) is a progressive degenerative disorder associated with repetitive traumatic brain injury. One of the primary defining neuropathological lesions in CTE, based on the first consensus conference, is the accumulation of hyperphosphorylated tau in gray matter sulcal depths. Post-mortem CTE studies have also reported myelin loss, axonal injury and white matter degeneration. Currently, the diagnosis of CTE is restricted to post-mortem neuropathological analysis. We hypothesized that high spatial resolution advanced diffusion MRI might be useful for detecting white matter microstructural changes directly adjacent to gray matter tau pathology...
March 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28212663/isolation-of-primary-microglia-from-the-human-post-mortem-brain-effects-of-ante-and-post-mortem-variables
#6
Mark R Mizee, Suzanne S M Miedema, Marlijn van der Poel, Adelia, Karianne G Schuurman, Miriam E van Strien, Jeroen Melief, Joost Smolders, Debbie A Hendrickx, Kirstin M Heutinck, Jörg Hamann, Inge Huitinga
Microglia are key players in the central nervous system in health and disease. Much pioneering research on microglia function has been carried out in vivo with the use of genetic animal models. However, to fully understand the role of microglia in neurological and psychiatric disorders, it is crucial to study primary human microglia from brain donors. We have developed a rapid procedure for the isolation of pure human microglia from autopsy tissue using density gradient centrifugation followed by CD11b-specific cell selection...
February 17, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28211314/procedure-for-horizontal-transfer-of-patient-derived-xenograft-tumors-to-eliminate-corynebacterium-bovis
#7
Christopher A Manuel Stacey M Bagby Julie A Reisinger Umarani Pugazhenthi Todd M Pitts Stephen B Keysar John J Arcaroli And Jori K Leszczynski
Human patient-derived xenograft (PDX) tumors, propagated in immunodeficient mice, are rapidly growing in use as amodelfor cancer research. Horizontal transfer between mice, without in vitro cell culture, allows these tumors to retainmany of their unique characteristics from their individual patient of origin. However, the immunodeficient mouse strainsused to grow these tumors are susceptible to numerous opportunistic pathogens, including Corynebacterium bovis. At ourinstitution, 2 in vivo tumor banks of PDX tumors had been maintained within nude mouse colonies enzootically infectedwith C...
February 16, 2017: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/28204962/the-clinical-anatomy-of-the-insertion-of-the-rotator-cuff-tendons
#8
M Vosloo, N Keough, M A De Beer
The rotator cuff (RC) insertions according to most anatomical texts are described as being separate from one another. However, clear fusion of the RC tendon fibres exists with prior studies showing this interdigitation forming a common, continuous insertion onto and around the lesser and greater tubercles (LT and GT) of the humerus. Current surgical repair methods (especially arthroscopic techniques) rarely mention or consider these connections during repair and suture anchor implantation. The general principles of RC surgery remain a controversial subject, due to various available techniques, surgeon experience and preference, and the contradicting success rates...
February 16, 2017: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/28196742/comparison-of-different-skin-preservation-methods-with-gamma-irradiation
#9
Linda Guerrero, Bernardo Camacho
Allografts are in constant demand, not only for burn victims, but also for all open wounds as "biological dressings". Tissue quality and security are two of the major concerns of Tissue Banks. There are limited studies published. There has been extensive discussion on the subject of preservation methods for cadaver skin. Most literature available comes from clinical reports. In this research, the authors compared 85% glycerolized non irradiated skin allografts with three glycerolized irradiated skin allografts (using different glycerol concentrations 50%, 70% and 85%)...
February 11, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28196298/human-to-human-transmission-of-brucella-a-systematic-review
#10
REVIEW
Felipe F Tuon, Regina B Gondolfo, Natacha Cerchiari
OBJECTIVE: The most common form of transmitting human brucellosis is through contaminated food or direct contact with infected animals. Human-to-human transmission (HHT) has been described as isolated case reports. The aim of this systematic review was to describe all cases of HHT of human brucellosis reported in the medical literature. METHODS: A literature search was conducted using PubMed, Scopus and Scielo databases using specific search terms published until March 2016...
February 14, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28190639/characterizing-white-matter-changes-in-chronic-schizophrenia-a-free-water-imaging-multi-site-study
#11
Lena K L Oestreich, Amanda E Lyall, Ofer Pasternak, Zora Kikinis, Dominick T Newell, Peter Savadjiev, Sylvain Bouix, Martha E Shenton, Marek Kubicki, Thomas J Whitford, Simon McCarthy-Jones
Diffusion tensor imaging (DTI) studies in chronic schizophrenia have found widespread but often inconsistent patterns of white matter abnormalities. These studies have typically used the conventional measure of fractional anisotropy, which can be contaminated by extracellular free-water. A recent free-water imaging study reported reduced free-water corrected fractional anisotropy (FAT) in chronic schizophrenia across several brain regions, but limited changes in the extracellular volume. The present study set out to validate these findings in a substantially larger sample...
February 10, 2017: Schizophrenia Research
https://www.readbyqxmd.com/read/28179988/ips-derived-mscs-from-an-expandable-bank-to-deliver-a-prodrug-converting-enzyme-that-limits-growth-and-metastases-of-human-breast-cancers
#12
M Ullah, Y Kuroda, T J Bartosh, F Liu, Q Zhao, C Gregory, R Reger, J Xu, R H Lee, D J Prockop
One attractive strategy to treat cancers is to deliver an exogenous enzyme that will convert a non-toxic compound to a highly toxic derivative. The strategy was tested with viral vectors but was disappointing because the efficiency of transduction into tumor cells was too low. Recent reports demonstrated that the limitation can be addressed by using tissue-derived mesenchymal stromal cells (MSCs) to deliver enzyme/prodrug systems that kill adjacent cancer cells through bystander effects. Here we addressed the limitation that tissue-derived MSCs vary in their properties and are difficult to generate in the large numbers needed for clinical applications...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28164608/alterations-of-vitamin-d-receptor-vdr-expression-profile-in-normal-and-malignant-breast-tissues
#13
Naghmeh Zhalehjoo, Yadollah Shakiba, Mojtaba Panjehpour
BACKGROUND: The actions of Vitamin D in different tissues, including breast tissue, are mediated by vitamin D receptor (VDR). Vitamin D has antitumor functions in the body; any changes in VDR expression can therefore affect the anticancer activities of Vitamin D. The present study was conducted to compare expression levels of VDR mRNA and protein in normal and tumor breast tissues. METHODS: Tumor and adjacent normal tissue samples from 30 patients with breast cancer were procured from the Iran National Tumor Bank of the Cancer Institute...
August 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28157770/global-adverse-event-reports-of-breast-implant-associated-alcl-an-international-review-of-40-government-authority-databases
#14
Dhivya R Srinivasa, Roberto N Miranda, Arminder Kaura, Ashleigh M Francis, Antonella Campanale, Rosaria Boldrini, Janette Alexander, Anand Deva, Paula Gravina, L Jeffrey Medeiros, Karen Nast, Charles E Butler, Mark W Clemens
BACKGROUND: An estimated 200 patients have been reported with breast implant associated anaplastic large cell lymphoma (BI-ALCL), a rare T-cell lymphoma developing around breast prostheses. The purpose of this study was to review federal database submissions relating to BI-ALCL in 37 countries representing the majority of breast implant markets worldwide with available adverse event reporting. METHODS: Database queries were performed for Australia, Brazil, Canada, China, Columbia, Japan, Mexico, National Competent Authorities of the European Member States, New Zealand, South Korea, and United States...
January 20, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28157689/differential-mineralization-of-human-dental-pulp-stem-cells-on-diverse-polymers
#15
Christian Apel, Patricia Buttler, Jochen Salber, Anandhan Dhanasingh, Sabine Neuss
In tissue engineering, biomaterials are used as scaffolds for spatial distribution of specific cell types. Biomaterials can potentially influence cell proliferation and extracellular matrix formation, both in positive and negative ways. The aim of the present study was to investigate and compare mineralized matrix production of human dental pulp stem cells (DPSC), cultured on 17 different well-characterized polymers. Osteogenic differentiation of DPSC was induced for 21 days on biomaterials using dexamethasone, L-ascorbic-acid-2-phosphate, and sodium β-glycerophosphate...
February 3, 2017: Biomedizinische Technik. Biomedical Engineering
https://www.readbyqxmd.com/read/28152737/financial-risk-sharing-agreements-using-options-to-make-marginal-benefits-cost-effective
#16
(no author information available yet)
: 41 Background: New cancer treatments have become increasingly costly. Although new drugs may be sufficiently cost-efficiency for a primary indication (i1) to receive funding, subsequent indications (ia) may not prove cost-effective. A financial risk-sharing agreement to increase access is proposed. A potential 2012 real-world implementation in Canada for pazopanib use in metastatic renal cell carcinoma (mRCC) as i1, and soft-tissue sarcoma (STS) as ia is presented. METHODS: The market value of an indication can be approximated by its net-present-value (NPV) of expected cash flows...
March 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28139472/microsurgical-tunica-albuginea-transplantation-in-an-animal-model
#17
Salvatore Sansalone, Carla Loreto, Rosario Leonardi, Giuseppe Vespasiani, Giuseppe Musumeci, Claudia Lombardo, Sergio Castorina, Venera Cardile, Rosario Caltabiano
Several andrological diseases require surgical repair or reconstruction of tunica albuginea, which envelops the corpora cavernosa penis. Despite intense research efforts involving a variety of biological materials, such as skin, muscle aponeurosis, human dura mater, tunica vaginalis, and pericardium, engineered tunica albuginea suitable for graft use is yet to be obtained. The study investigates microsurgical tunica albuginea allotransplantation in an animal model with the purpose of creation of an organ-specific tissue bank to store penile tissue, from cadaveric donors and male-to-female trans-sexual surgery, for allogeneic transplantation...
January 24, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28135684/evaluation-of-the-immunogenicity-of-human-ips-cell-derived-neural-stem-progenitor-cells-in-vitro
#18
Masahiro Ozaki, Akio Iwanami, Narihito Nagoshi, Jun Kohyama, Go Itakura, Hiroki Iwai, Soraya Nishimura, Yuichiro Nishiyama, Soya Kawabata, Keiko Sugai, Tsuyoshi Iida, Kohei Matsubayashi, Miho Isoda, Rei Kashiwagi, Yoshiaki Toyama, Morio Matsumoto, Hideyuki Okano, Masaya Nakamura
To achieve the goal of a first-in-human trial for human induced pluripotent stem cell (hiPSC)-based transplantation for the treatment of various diseases, allogeneic human leukocyte antigen (HLA)-matched hiPSC cell banks represent a realistic tool from the perspective of quality control and cost performance. Furthermore, considering the limited therapeutic time-window for acute injuries, including neurotraumatic injuries, an iPS cell bank is of potential interest. However, due to the relatively immunoprivileged environment of the central nervous system, it is unclear whether HLA matching is required in hiPSC-derived neural stem/progenitor cell (hiPSC-NS/PC) transplantation for the treatment of neurodegenerative diseases and neurotraumatic injuries...
January 19, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28129302/evaluation-and-quality-assessment-of-prestripped-preloaded-descemet-membrane-endothelial-keratoplasty-grafts
#19
Khoa D Tran, Philip K Dye, Kelly Odell, Joshua Galloway, Christopher G Stoeger, Michael D Straiko, Mark A Terry
PURPOSE: To determine graft quality and feasibility of Descemet membrane endothelial keratoplasty (DMEK) grafts that are prestripped and preloaded into injectors by eye bank technicians before shipping to surgeons. METHODS: DMEK grafts (n = 31) were prepared from donor corneas and preloaded into Straiko Modified Jones tubes and set inside viewing chambers filled with 20 mL of Optisol-GS. Preloaded grafts were evaluated using specular microscopy and slit-lamp biomicroscopy...
January 26, 2017: Cornea
https://www.readbyqxmd.com/read/28127872/quantitative-proteomics-of-breast-tumors-tissue-quality-assessment-to-clinical-biomarkers
#20
Yi Chen, David Britton, Elizabeth R Wood, Stephen Brantley, Anthony Magliocco, Ian Pike, John M Koomen
Liquid chromatography-selected reaction monitoring mass spectrometry (LC-SRM) is not only a proven tool for clinical chemistry, but also a versatile method to enhance the capability to quantify biomarkers for tumor biology research. As the treatment of cancer continues to evolve, the ability to assess multiple biomarkers to assign cancer phenotypes based on the genetic background and the signaling of the individual tumor becomes paramount to our ability to treat the patient. In breast cancer, the American Society of Clinical Oncology (ASCO) has defined biomarkers for patient assessment to guide selection of therapy: estrogen receptor, progesterone receptor, and the HER2/Neu receptor tyrosine kinase; therefore, these proteins were selected for LC-SRM assay development...
January 27, 2017: Proteomics
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