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https://www.readbyqxmd.com/read/28529456/ethyl-acetate-fraction-of-huogu-formula-inhibits-adipogenic-differentiation-of-bone-marrow-stromal-cells-via-the-bmp-and-wnt-signaling-pathways
#1
Xiangying Kong, Xiaomin Li, Cun Zhang, Liuluan Zhu, Chunfang Liu, Qingxia Qin, Cuiling Liu, Qianqian Wang, Jia Zhu, Xuan Wu, Hongye Wan, Weiheng Chen, Na Lin
Elevated adipogenesis of bone marrow stromal cells (BMSCs) is closely associated with non-traumatic osteonecrosis of femoral head (ONFH). Our previous studies have shown that Huogu (HG) formula was effective both in clinic experience and experimental ONFH. How HG impacts the differentiation of BMSCs and what is the underlying molecular mechanism remain largely unknown. Our results showed that ethyl acetate extract of HG (HGE) significantly decreased the adipocyte differentiation as determined by oil red staining, while slightly increased the ALP activity...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28528971/regulatory-effects-of-4-methoxychalcone-on-adipocyte-differentiation-through-ppar%C3%AE-activation-and-reverse-effect-on-tnf-%C3%AE-in-3t3-l1-cells
#2
Younho Han, Sung Ho Lee, Ik-Soo Lee, Kwang Youl Lee
Chalcones, the biosynthetic precursors of flavonoids and isoflavonoids abundant in edible plants, possess a number of pharmacological properties, and there is growing evidence that chalcone derivatives inhibit TNF-α mediated insulin resistance. The aim of the present study was to define the effects of 4-methoxychalcone (4-MC) on adipocyte differentiation and to determine the underlying molecular mechanism. We investigated the effects of 4-MC on adipocyte differentiation and lipid accumulation, and expression of adipogenic genes in 3T3-L1 cells...
May 18, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28516383/genetic-analysis-of-the-lf1-gene-that-controls-leaflet-number-in-soybean
#3
Soon-Chun Jeong, Ji Hong Kim, Dong Nyuk Bae
A high-resolution genetic map that was constructed for the Lf1 -residing region will provide valuable information for map-based cloning and genetic improvement efforts in soybean. Changes in leaf architecture as photosynthesis factories remain a major challenge for the improvement of crop productivity. Unlike most soybeans, which have compound leaves comprising three leaflets, the soybean Lf1 mutant has a high frequency of compound leaves with five leaflets in a partially dominant manner. Here, we generated a fine genetic map to determine the genetic basis of this multifoliolate leaf trait...
May 17, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28510189/multiple-regulatory-roles-of-ap2-erf-transcription-factor-in-angiosperm
#4
REVIEW
Chao Gu, Zhi-Hua Guo, Ping-Ping Hao, Guo-Ming Wang, Zi-Ming Jin, Shao-Ling Zhang
APETALA2/ethylene response factor (AP2/ERF) transcription factor (TF) is a superfamily in plant kingdom, which has been reported to be involved in regulation of plant growth and development, fruit ripening, defense response, and metabolism. As the final response gene in ethylene signaling pathway, AP2/ERF TF could feedback modulate phytohormone biosynthesis, including ethylene, cytokinin, gibberellin, and abscisic acid. Moreover, AP2/ERF TF also participates in response to the signals of auxin, cytokinin, abscisic acid, and jasmonate...
December 2017: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/28503742/the-ern1-transcription-factor-gene-is-a-target-of-the-ccamk-cyclops-complex-and-controls-rhizobial-infection-in%C3%A2-lotus-japonicus
#5
Marion R Cerri, Quanhui Wang, Paul Stolz, Jessica Folgmann, Lisa Frances, Katja Katzer, Xiaolin Li, Anne B Heckmann, Trevor L Wang, J Allan Downie, Andreas Klingl, Fernanda de Carvalho-Niebel, Fang Xie, Martin Parniske
Bacterial accommodation inside living plant cells is restricted to the nitrogen-fixing root nodule symbiosis. In many legumes, bacterial uptake is mediated via tubular structures called infection threads (ITs). To identify plant genes required for successful symbiotic infection, we screened an ethyl methanesulfonate mutagenized population of Lotus japonicus for mutants defective in IT formation and cloned the responsible gene, ERN1, encoding an AP2/ERF transcription factor. We performed phenotypic analysis of two independent L...
May 15, 2017: New Phytologist
https://www.readbyqxmd.com/read/28498935/the-association-of-changes-in-dna-methylation-with-temperature-dependent-sex-determination-in-cucumber
#6
Yun-Song Lai, Xiaohui Zhang, Wei Zhang, Di Shen, Haiping Wang, Yudong Xia, Yang Qiu, Jiangping Song, Chenchen Wang, Xixiang Li
Cucumber (Cucumis sativus L.) is characterized by its diverse and flexible sexual types. Here, we evaluated the effect of low temperature (LT) exposure on cucumber femaleness under short-day conditions. Shoot apices were subjected to whole-genome bisulfate sequencing (WGBS), mRNA-seq, and sRNA-seq. The results showed that temperature had a substantial and global impact on transposable element (TE)-related small RNA-directed DNA methylation (RdDM) mechanisms, resulting in large amounts of CHH-type cytosine demethylation...
May 12, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28485207/identification-and-positional-distribution-analysis-of-transcription-factor-binding-sites-for-genes-from-the-wheat-fl-cdna-sequences
#7
Zhen-Yong Chen, Xiao-Jiang Guo, Zhong-Xu Chen, Wei-Ying Chen, Ji-Rui Wang
The binding sites of transcription factors (TFs) in upstream DNA regions are called transcription factor binding sites (TFBSs). TFBSs are important elements for regulating gene expression. To date, there have been few studies on the profiles of TFBSs in plants. In total, 4,873 sequences with 5' upstream regions from 8530 wheat fl-cDNA sequences were used to predict TFBSs. We found 4572 TFBSs for the MADS TF family, which was twice as many as for bHLH (1951), B3 (1951), HB superfamily (1914), ERF (1820), and AP2/ERF (1725) TFs, and was approximately four times higher than the remaining TFBS types...
June 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28475114/melatonin-and-vitamin-d-interfere-with-the-adipogenic-fate-of-adipose-derived-stem-cells
#8
Valentina Basoli, Sara Santaniello, Sara Cruciani, Giorgio Carlo Ginesu, Maria Laura Cossu, Alessandro Palmerio Delitala, Pier Andrea Serra, Carlo Ventura, Margherita Maioli
Adipose-derived stem cells (ADSCs) represent one of the cellular populations resident in adipose tissue. They can be recruited under certain stimuli and committed to become preadipocytes, and then mature adipocytes. Controlling stem cell differentiation towards the adipogenic phenotype could have a great impact on future drug development aimed at counteracting fat depots. Stem cell commitment can be influenced by different molecules, such as melatonin, which we have previously shown to be an osteogenic inducer...
May 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28473839/overexpression-of-a-plasma-membrane-localized-sbsrp-like-protein-enhances-salinity-and-osmotic-stress-tolerance-in-transgenic-tobacco
#9
Pushpika Udawat, Rajesh K Jha, Avinash Mishra, Bhavanath Jha
An obligate halophyte, Salicornia brachiata grows in salt marshes and is considered to be a potential resource of salt- and drought-responsive genes. It is important to develop an understanding of the mechanisms behind enhanced salt tolerance. To increase this understanding, a novel SbSRP gene was cloned, characterized, over-expressed, and functionally validated in the model plant Nicotiana tabacum. The genome of the halophyte S. brachiata contains two homologs of an intronless SbSRP gene of 1,262 bp in length that encodes for a stress-related protein...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28469625/light-induced-variation-in-phenolic-compounds-in-cabernet-sauvignon-grapes-vitis-vinifera-l-involves-extensive-transcriptome-reprogramming-of-biosynthetic-enzymes-transcription-factors-and-phytohormonal-regulators
#10
Run-Ze Sun, Guo Cheng, Qiang Li, Yan-Nan He, Yu Wang, Yi-Bin Lan, Si-Yu Li, Yan-Rong Zhu, Wen-Feng Song, Xue Zhang, Xiao-Di Cui, Wu Chen, Jun Wang
Light environments have long been known to influence grape (Vitis vinifera L.) berry development and biosynthesis of phenolic compounds, and ultimately affect wine quality. Here, the accumulation and compositional changes of hydroxycinnamic acids (HCAs) and flavonoids, as well as global gene expression were analyzed in Cabernet Sauvignon grape berries under sunlight exposure treatments at different phenological stages. Sunlight exposure did not consistently affect the accumulation of berry skin flavan-3-ol or anthocyanin among different seasons due to climatic variations, but increased HCA content significantly at véraison and harvest, and enhanced flavonol accumulation dramatically with its timing and severity degree trend...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28455375/microrna172-plays-a-critical-role-in-wheat-spike-morphology-and-grain-threshability
#11
Juan Manuel Debernardi, Huiqiong Lin, Justin D Faris, Jorge Dubcovsky
Wheat domestication from wild species involved mutations in the Q gene. The q allele (wild wheats) is associated with elongated spikes and hulled grains, whereas the mutant Q allele (domesticated wheats) confers subcompact spikes and free-threshing grains. Previous studies showed that Q encodes an AP2-like transcription factor, but the causal polymorphism of the domestication traits remained unclear. Here we show that the interaction between microRNA172 (miR172) and the Q allele is reduced by an SNP in the miRNA binding site...
April 28, 2017: Development
https://www.readbyqxmd.com/read/28455374/new-alleles-of-the-wheat-domestication-gene-q-reveal-multiple-roles-in-growth-and-reproductive-development
#12
Julian R Greenwood, E Jean Finnegan, Nobuyoshi Watanabe, Ben Trevaskis, Steve M Swain
The advantages of free threshing in wheat led to the selection of the domesticated Q allele which is now present in almost all modern wheat varieties. Q and the pre-domestication allele, q, encode an AP2 transcription factor with the domesticated allele conferring a free threshing character and a subcompact (i.e. partially compact) inflorescence (spike). We demonstrate that mutations in the miR172-binding site of the Q gene are sufficient to increase transcript levels via a reduction in miRNA dependent degradation, consistent with the conclusion that a SNP in the miRNA-binding site of Q relative to q was essential in defining the modern Q allele...
April 28, 2017: Development
https://www.readbyqxmd.com/read/28447241/abbreviated-breast-dynamic-contrast-enhanced-mr-imaging-for-lesion-detection-and-characterization-the-experience-of-an-italian-oncologic-center
#13
Antonella Petrillo, Roberta Fusco, Mario Sansone, Marilena Cerbone, Salvatore Filice, Annamaria Porto, Maria Rosaria Rubulotta, Massimiliano D'Aiuto, Franca Avino, Maurizio Di Bonito, Gerardo Botti
BACKGROUND: To evaluate the performance of an abbreviated dynamic contrast-enhanced MR imaging (MRI) protocol for breast cancer detection; a comparison with the complete diagnostic protocol has been conducted. METHODS: A retrospective analysis on 508 patients was performed. Abbreviated protocol (AP) included one pre-contrast and the first post-contrast T1-weighted series. Complete protocol (CP) consisted of four post-contrast and one pre-contrast T1-weighted series...
April 26, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28446918/microrna-and-transcription-factor-key-players-in-plant-regulatory-network
#14
REVIEW
Abdul F A Samad, Muhammad Sajad, Nazaruddin Nazaruddin, Izzat A Fauzi, Abdul M A Murad, Zamri Zainal, Ismanizan Ismail
Recent achievements in plant microRNA (miRNA), a large class of small and non-coding RNAs, are very exciting. A wide array of techniques involving forward genetic, molecular cloning, bioinformatic analysis, and the latest technology, deep sequencing have greatly advanced miRNA discovery. A tiny miRNA sequence has the ability to target single/multiple mRNA targets. Most of the miRNA targets are transcription factors (TFs) which have paramount importance in regulating the plant growth and development. Various families of TFs, which have regulated a range of regulatory networks, may assist plants to grow under normal and stress environmental conditions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28443119/parsing-the-regulatory-network-between-small-rnas-and-target-genes-in-ethylene-pathway-in-tomato
#15
Yunxiang Wang, Qing Wang, Lipu Gao, Benzhong Zhu, Zheng Ju, Yunbo Luo, Jinhua Zuo
Small RNAs are a class of short non-coding endogenous RNAs that play essential roles in many biological processes. Recent studies have reported that microRNAs (miRNAs) are also involved in ethylene signaling in plants. LeERF1 is one of the ethylene response factors (ERFs) in tomato that locates in the downstream of ethylene signal transduction pathway. To elucidate the intricate regulatory roles of small RNAs in ethylene signaling pathway in tomato, the deep sequencing and bioinformatics methods were combined to decipher the small RNAs landscape in wild and sense-/antisense-LeERF1 transgenic tomato fruits...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28438500/ap2-%C3%AE-modulates-cystic-fibrosis-transmembrane-conductance-regulator-function-in-the-human-intestine
#16
Vandana Kumari, Shruti Desai, Nadia A Ameen
BACKGROUND: AP2 is a clathrin-based endocytic adaptor complex comprising α, β2, μ2 and σ2 subunits. μ2 regulates CFTR endocytosis. The α subunit interacts with CFTR in the intestine but its physiologic significance is unclear. METHODS: CFTR short circuit current was measured in intestinal T84 cells following shRNA knock down of AP2α (AP2αKD). Clathrin-coated structures (CCS) were immunolabeled and quantified in AP2αKD intestinal Caco2BBe (C2BBe) cells. GST tagged human AP2α appendage domain was cloned and its interaction with CFTR determined by GST pull down assay...
May 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28437481/firemaster%C3%A2-550-and-its-components-isopropylated-triphenyl-phosphate-and-triphenyl-phosphate-enhance-adipogenesis-and-transcriptional-activity-of-peroxisome-proliferator-activated-receptor-ppar%C3%AE-on-the-adipocyte-protein-2-ap2-promoter
#17
Emily W Y Tung, Shaimaa Ahmed, Vian Peshdary, Ella Atlas
Firemaster® 550 (FM550) is a chemical mixture currently used as an additive flame retardant in commercial products, and is comprised of 2-ethylhexyl-2,3,4,5-tertrabromobenzoate (TBB), bis(2-ethylhexyl) tetrabromophthalate (TBPH), triphenyl phosphate (TPP), and isopropylated triphenyl phosphate (IPTP). Animal and in vitro studies suggest that FM550, TPP and IPTP may have adipogenic effects and may exert these effects through PPARγ activation. Using murine 3T3-L1 preadipocytes, we investigated the detailed expression of transcription factors and adipogenic markers in response to FM550 and its components...
2017: PloS One
https://www.readbyqxmd.com/read/28433029/early-stages-of-clathrin-aggregation-at-a-membrane-in-coarse-grained-simulations
#18
M Giani, W K den Otter, W J Briels
The self-assembly process of clathrin coated pits during endocytosis has been simulated by combining and extending coarse grained models of the clathrin triskelion, the adaptor protein AP2, and a flexible network membrane. The AP2's core, upon binding to membrane and cargo, releases a motif that can bind clathrin. In conditions where the core-membrane-cargo binding is weak, the binding of this motif to clathrin can result in a stable complex. We characterize the conditions and mechanisms resulting in the formation of clathrin lattices that curve the membrane, i...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28424783/peptide-fraction-poh2-exerts-antiadipogenic-activity-through-inhibition-of-c-ebp-%C3%AE-and-ppar-%C3%AE-expression-in-3t3-l1-adipocytes
#19
Thi Tuyet Nhung Nguyen, Thi Thu Ha, Thi Hoa Nguyen, Thi Hien Vu, Nam Hai Truong, Hoang Ha Chu, Dong Van Quyen
Many studies have comprehensively examined the venom of Ophiophagus hannah snake. Its venom comprises different compounds exhibiting a wide range of pharmacological activities. In this investigation, four peptide fractions (PFs), ranging from 3 kDa to 10 kDa, isolated from the Vietnamese snake venom of O. hannah were separated by HPLC and investigated for their inhibitory activity on adipogenesis in 3T3-L1 adipocytes. The most effective PF was then further purified, generating two peptides, pOh1 and pOh2...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28421097/development-of-incompletely-fused-carpels-in-maize-ovary-revealed-by-mirna-target-gene-and-phytohormone-analysis
#20
Hongping Li, Ting Peng, Qun Wang, Yufeng Wu, Jianfeng Chang, Moubiao Zhang, Guiliang Tang, Chaohai Li
Although the molecular basis of carpel fusion in maize ovary development remains largely unknown, increasing evidence suggests a critical role of microRNAs (miRNAs). In this study, a combination of miRNA sequencing, degradome and physiological analyses was used to characterize carpel fusion development in maize ovaries showing incompletely (IFC) and completely fused carpels (CFC). A total of 162 known miRNAs distributed across 33 families were identified, of which 20 were differentially expressed. In addition, 53 miRNA candidates were identified, of which 10 were differentially expressed in the IFC and CFC ovaries...
2017: Frontiers in Plant Science
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