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https://www.readbyqxmd.com/read/28816006/cd38-deficiency-suppresses-adipogenesis-and-lipogenesis-in-adipose-tissues-through-activating-sirt1-ppar%C3%AE-signaling-pathway
#1
Ling-Fang Wang, Lian-Jie Miao, Xiao-Nv Wang, Cong-Cong Huang, Yi-Song Qian, Xuan Huang, Xiao-Lei Wang, Wan-Zhu Jin, Guang-Ju Ji, Mingui Fu, Ke-Yu Deng, Hong-Bo Xin
It has been recently reported that CD38 was highly expressed in adipose tissues from obese people and CD38-deficient mice were resistant to high-fat diet (HFD)-induced obesity. However, the role of CD38 in the regulation of adipogenesis and lipogenesis is unknown. In this study, to explore the roles of CD38 in adipogenesis and lipogenesis in vivo and in vitro, obesity models were generated with male CD38(-/-) and WT mice fed with HFD. The adipocyte differentiations were induced with MEFs from WT and CD38(-/-) mice, 3T3-L1 and C3H10T1/2 cells in vitro...
August 16, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28815933/an-%C3%AE-iib-%C3%AE-3-antagonist-prevents-thrombosis-without-causing-fc%C3%AE-riia-mediated-thrombocytopenia
#2
Yu-Ju Kuo, Ying-Ru Chen, Chun-Chieh Hsu, Hui-Chin Peng, Tur-Fu Huang
BACKGROUND: Thrombocytopenia, a common side effect of Arg-Gly-Asp (RGD)-mimetic antiplatelet drugs, is associated with drug-dependent antibodies (DDAbs) that recognize conformation-altered integrin αIIb β3 . OBJECTIVE: To explore the correlation between αIIb β3 binding epitopes and induction of DDAb binding to conformation-altered αIIb β3 , we examined whether two purified disintegrins TMV-2 and TMV-7 with distinct binding motifs that have different effects on induction of αIIb β3 conformational change and platelet aggregation in the presence of AP2, an IgG1 inhibitory monoclonal antibody (mAb) raised against αIIb β3 ...
August 16, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28802120/discovery-of-pentacyclic-triterpene-3%C3%AE-ester-derivatives-as-a-new-class-of-cholesterol-ester-transfer-protein-inhibitors
#3
Dongyin Chen, Xin Huang, Hongwen Zhou, Hanqiong Luo, Pengfei Wang, Yongzhi Chang, Xinyi He, Suiying Ni, Qingqing Shen, Guoshen Cao, Hongbin Sun, Xiaoan Wen, Jun Liu
A series of pentacyclic triterpene 3β-ester derivatives were designed, synthesized and evaluated as a new class of cholesteryl ester transfer protein (CETP) inhibitors for the treatment of dyslipidemia. In vitro screening assay showed that 5 out of 30 compounds displayed moderate inhibiting human CETP activity with IC50s less than 10 μM. Among them, compound 20 (IC50 = 2.3 μM) had the most potent biological activity, and effectively ameliorated plasma lipid levels of human adipose tissue specific CETP transgenic (ap2-CETPTg) mice and guinea pigs...
August 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28796196/two-c3h-type-zinc-finger-protein-genes-cpczf1-and-cpczf2-from-chimonanthus-praecox-affect-stamen-development-in-arabidopsis
#4
Huamin Liu, Renwei Huang, Jing Ma, Shunzhao Sui, Yulong Guo, Daofeng Liu, Zhineng Li, Yechun Lin, Mingyang Li
Wintersweet (Chimonanthus praecox) is a popular garden plant because of its flowering time, sweet fragrance, and ornamental value. However, research into the molecular mechanism that regulates flower development in wintersweet is still limited. In this study, we sought to investigate the molecular characteristics, expression patterns, and potential functions of two C3H-type zinc finger (CZF) protein genes, CpCZF1 and CpCZF2, which were isolated from the wintersweet flowers based on the flower developmental transcriptome database...
August 10, 2017: Genes
https://www.readbyqxmd.com/read/28793320/microsomal-triglyceride-transfer-protein-contributes-to-lipid-droplet-maturation-in-adipocytes
#5
Larry L Swift, Joseph D Love, Carla M Harris, Benny H Chang, W Gray Jerome
Previous studies in our laboratory have established the presence of MTP in both white and brown adipose tissue in mice as well as in 3T3-L1 cells. Additional studies demonstrated an increase in MTP levels as 3T3-L1 cells differentiate into adipocytes concurrent with the movement of MTP from the juxtanuclear region of the cell to the surface of lipid droplets. This suggested a role for MTP in lipid droplet biogenesis and/or maturation. To probe the role of MTP in adipocytes, we used a Cre-Lox approach with aP2-Cre and Adipoq-Cre recombinase transgenic mice to knock down MTP expression in brown and white fat of mice...
2017: PloS One
https://www.readbyqxmd.com/read/28783504/puberty-arises-with-testicular-alterations-and-defective-amh-expression-in-rams-prenatally-exposed-to-testosterone
#6
S E Recabarren, M Recabarren, D Sandoval, A Carrasco, V Padmanabhan, R Rey, H G Richter, C C Perez-Marin, T Sir-Petermann, P P Rojas-Garcia
The male gonadal tissue can be a sensitive target to the reprogramming effects of testosterone (T) during prenatal development. We have demonstrated that male lambs born to dams receiving T during pregnancy-a model system to the polycystic ovary syndrome (PCOS)-show a decreased number of germ cells early in life, and when adult, a reduced amount of sperm and ejaculate volume. These findings are a key to put attention to the male offspring of women bearing PCOS, as they are exposed to increased levels of androgen during pregnancy which can reprogram their reproductive outcome...
June 23, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28769963/tissue-specific-floral-transcriptome-analysis-of-the-sexually-deceptive-orchid-chiloglottis-trapeziformis-provides-insights-into-the-biosynthesis-and-regulation-of-its-unique-uv-b-dependent-floral-volatile-chiloglottone-1
#7
Darren C J Wong, Ranamalie Amarasinghe, Claudia Rodriguez-Delgado, Rodney Eyles, Eran Pichersky, Rod Peakall
The Australian sexually deceptive orchid, Chiloglottis trapeziformis, employs a unique UV-B-dependent floral volatile, chiloglottone 1, for specific male wasp pollinator attraction. Chiloglottone 1 and related variants (2,5-dialkylcyclohexane-1,3-diones), represent a unique class of specialized metabolites presumed to be the product of cyclization between two fatty acid (FA) precursors. However, the genes involved in the biosynthesis of precursors, intermediates, and transcriptional regulation remains to be discovered...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28769962/global-gene-expression-analysis-reveals-crosstalk-between-response-mechanisms-to-cold-and-drought-stresses-in-cassava-seedlings
#8
Shuxia Li, Xiang Yu, Zhihao Cheng, Xiaoling Yu, Mengbin Ruan, Wenbin Li, Ming Peng
Abiotic stress negatively impacts cassava (Manihot esculenta) growth and yield. Several molecular mechanisms of plant response to cold and drought have been identified and described in the literature, however, little is known about the crosstalk of the responses of cassava to these two stresses. To elucidate this question, transcriptome analysis of cassava seedlings under cold or PEG-simulated drought stress treatment was performed. Our results showed that 6103 and 7462 transcripts were significantly regulated by cold and drought stress, respectively...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28763300/ablation-of-adipose-ho-1-expression-increases-white-fat-over-beige-fat-through-inhibition-of-mitochondrial-fusion-and-of-pgc1%C3%AE-in-female-mice
#9
Shailendra P Singh, Ilana Grant, Aliza Meissner, Attallah Kappas, Nader G Abraham
Background Hmox1 plays an important role in the regulation of mitochondrial bioenergetics and function by regulating cellular heme-derived CO and bilirubin. Previous studies have demonstrated that global disruption of HO-1 in humans and mice resulted in severe organ dysfunction. Methods We investigated the potential role of adipose-specific-HO-1 genetic ablation on adipose tissue function, mitochondrial quality control and energy expenditure by generating an adipo-HO-1 knockout mouse model (Adipo-HO-1-/-) and, in vitro, adipocyte cells in which HO activity was inhibited...
August 1, 2017: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/28751675/cell-cycle-arrest-in-mature-adipocytes-impairs-bat-development-but-not-wat-browning-and-reduces-adaptive-thermogenesis-in-mice
#10
Yuko Okamatsu-Ogura, Keigo Fukano, Ayumi Tsubota, Junko Nio-Kobayashi, Kyoko Nakamura, Masami Morimatsu, Hiroshi Sakaue, Masayuki Saito, Kazuhiro Kimura
We previously reported brown adipocytes can proliferate even after differentiation. To test the involvement of mature adipocyte proliferation in cell number control in fat tissue, we generated transgenic (Tg) mice over-expressing cell-cycle inhibitory protein p27 specifically in adipocytes, using the aP2 promoter. While there was no apparent difference in white adipose tissue (WAT) between wild-type (WT) and Tg mice, the amount of brown adipose tissue (BAT) was much smaller in Tg mice. Although BAT showed a normal cellular morphology, Tg mice had lower content of uncoupling protein 1 (UCP1) as a whole, and attenuated cold exposure- or β3-adrenergic receptor (AR) agonist-induced thermogenesis, with a decrease in the number of mature brown adipocytes expressing proliferation markers...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28744297/dewax-transcription-factor-is-involved-in-resistance-to-botrytis-cinerea-in-arabidopsis-thaliana-and-camelina-sativa
#11
Seulgi Ju, Young Sam Go, Hyo Ju Choi, Jeong Mee Park, Mi Chung Suh
The cuticle of land plants is the first physical barrier to protect their aerial parts from biotic and abiotic stresses. DEWAX, an AP2/ERF-type transcription factor, negatively regulates cuticular wax biosynthesis. In this study, we investigated the resistance to Botrytis cinerea in Arabidopsis thaliana and Camelina sativa overexpressing DEWAX and in Arabidopsis dewax mutant. Compared to wild type (WT) leaves, Arabidopsis DEWAX OX and dewax leaves were more and less permeable to toluidine blue dye, respectively...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28740499/multi-omics-and-integrated-network-analyses-reveal-new-insights-into-the-systems-relationships-between-metabolites-structural-genes-and-transcriptional-regulators-in-developing-grape-berries-vitis-vinifera-l-exposed-to-water-deficit
#12
Stefania Savoi, Darren C J Wong, Asfaw Degu, Jose C Herrera, Barbara Bucchetti, Enrico Peterlunger, Aaron Fait, Fulvio Mattivi, Simone D Castellarin
Grapes are one of the major fruit crops and they are cultivated in many dry environments. This study comprehensively characterizes the metabolic response of grape berries exposed to water deficit at different developmental stages. Increases of proline, branched-chain amino acids, phenylpropanoids, anthocyanins, and free volatile organic compounds have been previously observed in grape berries exposed to water deficit. Integrating RNA-sequencing analysis of the transcriptome with large-scale analysis of central and specialized metabolites, we reveal that these increases occur via a coordinated regulation of key structural pathway genes...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28724758/the-shcd-phosphotyrosine-adaptor-subverts-canonical-egf-receptor-trafficking
#13
Melanie K B Wills, Hayley R Lau, Nina Jones
Shc family signalling adaptors connect activated transmembrane receptors to proximal effectors, and most also contain a sequence involved in clathrin-mediated receptor endocytosis. Notably, this AP2 adaptin binding motif (AD) is absent from the ShcD homolog, which also uniquely promotes ligand-independent phosphorylation of the Epidermal Growth Factor (EGF) Receptor (EGFR). We now report that cultured cells expressing ShcD exhibit reduced EGF uptake, commensurate with a decrease in EGFR surface presentation...
July 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28708578/hyperglycemia-and-advanced-glycation-end-products-ages-suppress-the-differentiation-of-3t3-l1-preadipocytes
#14
Chia-Chu Chang, Chen-Yu Chen, Geen-Dong Chang, Ting-Huan Chen, Woan-Ling Chen, Hui-Chin Wen, Chih-Yang Huang, Chung-Ho Chang
Aging is characterized by mild hyperglycemia and accumulation of advanced glycation end products (AGEs). Effects of chronic exposure to hyperglycemia or AGEs on the adipogenic differentiation of 3T3-L1 preadipocytes remain unclear. We examined the chronic effect of AGEs and high glucose on the differentiation of 3T3-L1 cells by culturing 3T3-L1 cells in the presence of AGEs or 25 mM glucose for 1 month. Chronic incubation of 3T3-L1 cells with AGEs or high glucose blocked their differentiation into mature adipocytes as evidenced by reduced levels of adipocyte markers such as accumulated oil droplets, GPDH, aP2, adiponectin and of adipogenesis regulators PPARγ and C/EBPα...
July 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28704952/the-inhibitory-effect-of-tartary-buckwheat-extracts-on-adipogenesis-and-inflammatory-response
#15
Mak-Soon Lee, Yoonjin Shin, Sunyoon Jung, Seog-Young Kim, Young-Hee Jo, Chong-Tai Kim, Min-Kyu Yun, Sung-Jin Lee, Johann Sohn, Heui-Jong Yu, Yangha Kim
Tartary buckwheat (Fagopyrum tataricum) has been established globally as a nutritionally important food item, particularly owing to high levels of bioactive compounds such as rutin. This study investigated the effect of tartary buckwheat extracts (TBEs) on adipogenesis and inflammatory response in 3T3-L1 cells. TBEs inhibited lipid accumulation, triglyceride content, and glycerol-3-phosphate dehydrogenase (GPDH) activity during adipocyte differentiation of 3T3 L1 cells. The mRNA levels of genes involved in fatty acid synthesis, such as peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer binding protein-α (CEBP-α), adipocyte protein 2 (aP2), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and stearoylcoenzyme A desaturase-1 (SCD-1), were suppressed by TBEs...
July 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28700604/molecular-characterization-of-the-acquisition-of-longevity-during-seed-maturation-in-soybean
#16
Juliana Joice Pereira Lima, Julia Buitink, David Lalanne, Rubiana Falopa Rossi, Sandra Pelletier, Edvaldo Aparecido Amaral da Silva, Olivier Leprince
Seed longevity, defined as the ability to remain alive during storage, is an important agronomic factor. Poor longevity negatively impacts seedling establishment and consequently crop yield. This is particularly problematic for soybean as seeds have a short lifespan. While the economic importance of soybean has fueled a large number of transcriptome studies during embryogenesis and seed filling, the mechanisms regulating seed longevity during late maturation remain poorly understood. Here, a detailed physiological and molecular characterization of late seed maturation was performed in soybean to obtain a comprehensive overview of the regulatory genes that are potentially involved in longevity...
2017: PloS One
https://www.readbyqxmd.com/read/28692330/regulation-of-cell-wall-genes-in-response-to-defective-kernel1-dek1-induced-cell-wall-changes
#17
Dhika Amanda, Monika S Doblin, Roberta Galletti, Antony Bacic, Gwyneth C Ingram, Kim L Johnson
Defective Kernel1 (DEK1) is a plant-specific calpain involved in epidermis specification and maintenance. DEK1 regulation of the epidermal cell wall is proposed to be key to ensure tissue integrity and coordinated growth. Changes in the expression of DEK1 are correlated with changes in the expression of cell wall-related genes. For example, we have found that Lipid transfer protein 3 (LTP3), EXPANSIN 11 (EXP11), and an AP2 transcription factor (AP2TF) are misexpressed in plants with constitutively altered levels of DEK1 activity...
July 10, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28684042/signal-dependent-transport-of-a-membrane-cargo-from-early-endosomes-to-recycling-endosomes
#18
Ismail S Mahmoud, Jade Louber, Steve K Dower, Anne M Verhagen, Paul A Gleeson
Many membrane cargoes undergo endocytosis and intracellular recycling to the plasma membrane via the early endosomes and the recycling endosomes. However whether specific sorting signals are required for transport from early endosomes to recycling endosomes is not known and the current view is that transport to the recycling endosomes is by a passive default process. Here we show that the cytoplasmic tail of the neonatal Fc receptor (FcRn) contains discrete signals for endocytosis and for sorting to the recycling endosomes...
June 17, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28681139/a-novel-ap2-erf-family-transcription-factor-from-glycine-soja-gserf71-is-a-dna-binding-protein-that-positively-regulates-alkaline-stress-tolerance-in-arabidopsis
#19
Yang Yu, Xiangbo Duan, Xiaodong Ding, Chao Chen, Dan Zhu, Kuide Yin, Lei Cao, Xuewei Song, Pinghui Zhu, Qiang Li, Zaib Un Nisa, Jiyang Yu, Jianying Du, Yu Song, Huiqing Li, Beidong Liu, Yanming Zhu
Here we first found that GsERF71, an ERF factor from wild soybean could increase plant alkaline stress tolerance by up-regulating H+-ATPase and by modifing the accumulation of Auxin. Alkaline soils are widely distributed all over the world and greatly limit plant growth and development. In our previous transcriptome analyses, we have identified several ERF (ethylene-responsive factor) genes that responded strongly to bicarbonate stress in the roots of wild soybean G07256 (Glycine soja). In this study, we cloned and functionally characterized one of the genes, GsERF71...
July 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28671595/anti-obesity-effect-of-the-above-ground-part-of-valeriana-dageletiana-nakai-ex-f-maek-extract-in-high-fat-diet-induced-obese-c57bl-6n-mice
#20
Zhiqiang Wang, Seung Hwan Hwang, Ju Hee Kim, Soon Sung Lim
Valeriana dageletiana Nakai ex F. Maek (VD) has been used as traditional medicine for the treatment of restlessness and sleeping disorders. However, it is still unclear whether obesity in mice can be altered by diet supplementation with VD. In this study, we first investigated the influences of VD on the accumulation of lipid content in 3T3-L1 cells; and the results showed that the above-ground VD extracts (VDAE) suppressed the differentiation of 3T3-L1 preadipocytes in a concentration-dependent manner without cytotoxicity...
July 2, 2017: Nutrients
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