keyword
MENU ▼
Read by QxMD icon Read
search

Enamel regeneration

keyword
https://www.readbyqxmd.com/read/29779269/-the-applications-of-periodontal-gingival-surgery-%C3%A2-alternative-materials
#1
Er-Jia Mao
The main purposes of periodontal graft surgery include achieving root coverage, improving the clinical attachment level and keratinized tissue, and advancing the procedure of periodontal plastic surgery. Autogenous graft, such as subepithelial connective tissue graft-based procedure, provide the best outcomes for mean and complete root coverage, as well as increase in keratinized tissue. However, a disadvantage of the procedure is in the location of the operation itself: the additional surgical site (palate)...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29767434/regenerative-surgical-therapy-for-peri-implantitis-using-deproteinized-bovine-bone-mineral-with-10-collagen-enamel-matrix-derivative-and-doxycycline-a-prospective-3-year-cohort-study
#2
Faustino Mercado, Stephen Hamlet, Saso Ivanovski
OBJECTIVES: There is limited evidence regarding the long-term efficacy of regenerative treatment for peri-implantitis. The aim of this study was to evaluate a combination therapy of deproteinized bovine bone mineral with 10% collagen (DBBMC), enamel matrix derivative (EMD) and Doxycycline in the regeneration of bone defects associated with peri-implantitis. METHODS: Thirty patients diagnosed with peri-implantitis (BoP/suppuration, probing depth greater than 4 mm, minimum radiographic bone loss of 20%, at least 2 years in function) were enrolled in the study...
May 16, 2018: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/29748557/recombinant-amelogenin-regulates-the-bioactivity-of-mouse-cementoblasts-in-vitro
#3
Sema S Hakki, S Buket Bozkurt, Emre Türkay, Michel Dard, Nuhan Purali, Werner Götz
Amelogenin (AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG (rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts (OCCM-30) were treated with different concentrations (0.1, 1, 10, 100, 1000, 10,000, 100,000 ng · mL-1 ) of recombinant human AMG (rhAMG) and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein (BSP), osteocalcin (OCN), collagen type I (COL I), osteopontin (OPN), runt-related transcription factor 2 (Runx2), cementum attachment protein (CAP), and alkaline phosphatase (ALP) genes using quantitative RT-PCR...
May 9, 2018: International Journal of Oral Science
https://www.readbyqxmd.com/read/29720250/efficient-induction-of-functional-ameloblasts-from-human-keratinocyte-stem-cells
#4
Xuefeng Hu, Jyh-Wei Lee, Xi Zheng, Junhua Zhang, Xin Lin, Yingnan Song, Bingmei Wang, Xiaoxiao Hu, Hao-Hueng Chang, Yiping Chen, Chun-Pin Lin, Yanding Zhang
BACKGROUND: Although adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the regenerated enamel retains a running pattern of prism identical to and acquires mechanical properties comparable with human enamel indeed warrants further study. METHODS: Cultured human keratinocyte stem cells (hKSCs) were treated with fibroblast growth factor 8 (FGF8) and Sonic hedgehog (SHH) for 18 h or 36 h prior to being recombined with E13...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29683502/the-c-terminus-of-the-amelogenin-peptide-influences-the-proliferation-of-human-bone-marrow-mesenchymal-stem-cells
#5
Ryo Kunimatsu, Tetsuya Awada, Yuki Yoshimi, Kazuyo Ando, Naoto Hirose, Yuki Tanne, Keisuke Sumi, Kotaro Tanimoto
BACKGROUND: Amelogenins are a family of enamel matrix proteins that are important for formation of enamel. Amelogenins may induce division of mesenchymal stem cells (MSCs), among others. Recently, the C-terminus of the amelogenin peptide (AMG-CP) has been shown to enhance the proliferation of cementoblast lineage cells. The role of the amelogenin peptide on the proliferation of human MSCs and related alterations in the intracellular signaling pathway were studied. METHODS: MSCs were exposed to AMG-CP in vitro...
April 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29623301/peptide-based-bioinspired-approach-to-regrowing-multilayered-aprismatic-enamel
#6
Kaushik Mukherjee, Qichao Ruan, Steven Nutt, Jinhui Tao, James J De Yoreo, Janet Moradian-Oldak
The gradual discovery of functional domains in native enamel matrix proteins has enabled the design of smart bioinspired peptides for tooth enamel mimetics and repair. In this study, we expanded upon the concept of biomineralization to design smaller amelogenin-inspired peptides with conserved functional domains for clinical translation. The synthetic peptides displayed a characteristic nanostructured scaffold reminiscent of 'nanospheres' seen in the enamel matrix and effectively controlled apatite nucleation in vitro resulting in the formation of smaller crystallites...
March 31, 2018: ACS Omega
https://www.readbyqxmd.com/read/29590225/periodontal-regenerative-treatment-of-intrabony-defects-in-the-esthetic-zone-using-modified-vestibular-incision-subperiosteal-tunnel-access-m-vista
#7
Babak Najafi, Parisa Kheirieh, Alireza Torabi, Emil G Cappetta
Periodontal regenerative treatment of intrabony defects in the esthetic zone often poses a significant challenge to clinicians. Various materials and techniques have been proposed to achieve periodontal regeneration. This article presents modified vestibular incision subperiosteal tunnel access (M-VISTA) for treatment of intrabony defects in the esthetic zone. Two cases were treated using the proposed approach with enamel matrix derivatives and demineralized freeze-dried bone allograft to optimize the regenerative outcomes without affecting the soft tissue profile in the esthetic zone...
March 28, 2018: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/29580724/application-of-enamel-matrix-derivative-emdogain-in-endodontic-therapy-a-comprehensive-literature-review
#8
REVIEW
Howard H Wang, Nima D Sarmast, Elham Shadmehr, Nikola Angelov, Shahrokh Shabahang, Mahmoud Torabinejad
INTRODUCTION: Enamel matrix derivative (EMD) is a protein extract used for the treatment of periodontal defects and soft tissue recession. Its use in endodontics has been a subject of exploration, especially in regenerative procedures. The aim of this review was to evaluate the current literature available on the application of EMD in the field of endodontics. METHODS: An initial literature search of databases using different combinations of the search terms yielded 1089 articles...
March 23, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29533942/biology-explaining-tooth-repair-and-regeneration-a-mini-review
#9
Anamaria Balic
The tooth is an intricate composition of precisely patterned, mineralized matrices and soft tissues. Mineralized tissues include enamel (produced by the epithelial cells called ameloblasts), dentin and cementum (produced by mesenchymal cells called odontoblasts and cementoblasts, respectively), and soft tissues, which include the dental pulp and the periodontal ligament along with the invading nerves and blood vessels. It was perceived for a very long time that teeth primarily serve an esthetical function. In recent years, however, the role of healthy teeth, as well as the impact of oral health on general well-being, became more evident...
March 13, 2018: Gerontology
https://www.readbyqxmd.com/read/29517915/polylactic-acid-nanopillar-array-driven-osteogenic-differentiation-of-human-adipose-derived-stem-cells-determined-by-pillar-diameter
#10
Shan Zhang, Baojin Ma, Feng Liu, Jiazhi Duan, Shicai Wang, Jichuan Qiu, Dong Li, Yuanhua Sang, Chao Liu, Duo Liu, Hong Liu
Numerous studies have determined that physical cues, especially the nanotopography of materials, play key roles in directing stem cell differentiation. However, most research on nanoarrays for stem cell fate regulation is based on nonbiodegradable materials, such as silicon wafers, TiO2 , and poly(methyl methacrylate), which are rarely used as tissue engineering biomaterials. In this study, we prepared biodegradable polylactic acid (PLA) nanopillar arrays with different diameters but the same center-to-center distance using a series of anodic aluminum oxide nanowell arrays as templates...
April 11, 2018: Nano Letters
https://www.readbyqxmd.com/read/29485179/combining-enamel-matrix-proteins-with-mechanical-stimuli-potentiates-human-periodontal-ligament-fibroblasts-proliferation-and-periodontium-remodeling
#11
Rui Zou, Wanting Wan, Jingjing Li, Chanyuan Du, Yijie Wang, Tian Qian, Lin Niu
BACKGROUND: Collagen I (Col-I) and matrix metalloproteinase-1 (MMP-1) have been implicated in the regeneration and remodeling of the periodontium. Studies have shown that enamel matrix proteins (EMPs) and mechanical stimuli can promote the synthesis and degradation, respectively, of Col-I and MMP-1. However, the effects of the combination of EMPs and mechanical stimuli on human periodontal ligament are not known. OBJECTIVE: Our aim was to test the combined effects of EMPs and mechanical stimuli on the proliferation of human periodontal ligament fibroblasts (HPDLFs) and Col-I and MMP-1 mRNA expression...
February 27, 2018: Histology and Histopathology
https://www.readbyqxmd.com/read/29322600/using-enamel-matrix-derivative-to-improve-treatment-efficacy-in-periodontal-furcation-defects
#12
REVIEW
Reza Masaeli, Kavosh Zandsalimi, Zahra Lotfi, Lobat Tayebi
PURPOSE: Furcations are complicated periodontal defects. Untreated furcations lead to loss of the involved teeth and supporting tissues. It has been demonstrated that regenerative biomaterials are beneficial in reconstruction of the bone surrounding furcation-affected teeth. These biomaterials range from bone grafts and nonresorbable/resorbable barrier membranes to biologics that are able to trigger inactive regenerative processes in periodontal tissues. Selection of appropriate material(s) to treat furcations is challenging...
January 11, 2018: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/29057222/identification-of-trombospondin-1-as-a-novel-amelogenin-interactor-by-functional-proteomics
#13
Angela Capolupo, Chiara Cassiano, Agostino Casapullo, Giuseppina Andreotti, Maria V Cubellis, Andrea Riccio, Raffaele Riccio, Maria C Monti
Amelogenins are a set of low molecular-weight enamel proteins belonging to a group of extracellular matrix (ECM) proteins with a key role in tooth enamel development and in other regeneration processes, such as wound healing and angiogenesis. Since only few data are actually available to unravel amelogenin mechanism of action in chronic skin healing restoration, we moved to the full characterization of the human amelogenin isoform 2 interactome in the secretome and lysate of Human Umbilical Vein Endothelial cells (HUVEC), using a functional proteomic approach...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29044527/effect-of-the-combined-use-of-enamel-matrix-derivative-and-atelocollagen-sponge-scaffold-on-osteoblastic-differentiation-of-mouse-induced-pluripotent-stem-cells-in-vitro
#14
Y Hisanaga, E Suzuki, H Aoki, M Sato, A Saito, A Saito, T Azuma
BACKGROUND AND OBJECTIVE: Induced pluripotent stem cells (iPSCs) are a candidate cell source in periodontal regenerative therapy. Enamel matrix derivative (EMD) has been shown to regenerate periodontal tissues, and atelocollagen sponge (ACS) is considered a suitable scaffold or carrier for growth factors. This study aimed to investigate the effect of combined use of EMD and an ACS scaffold on cell behaviors and differentiation of mouse iPSCs (miPSCs) in vitro. MATERIAL AND METHODS: Following embryonic body formation from miPSCs, dissociated cells (miPS-EB-derived cells) were seeded onto ACS with or without EMD, and cultured in osteoblast differentiation medium...
April 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29027917/porcine-dental-epithelial-cells-differentiated-in-a-cell-sheet-constructed-by-magnetic-nanotechnology
#15
Wataru Koto, Yoshinori Shinohara, Kazuyuki Kitamura, Takanori Wachi, Seicho Makihira, Kiyoshi Koyano
Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology...
October 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28969268/comparative-evaluation-of-platelet-rich-fibrin-and-dehydrated-amniotic-membrane-for-the-treatment-of-gingival-recession-a-clinical-study
#16
Ankita Jain, Gagan Rajesh Jaiswal, Kanteshwari Kumathalli, Rajesh Kumar, Alisha Singh, Aakrti Sarwan
INTRODUCTION: The main objective of any therapeutic intervention aimed at root coverage is to restore the tissue margin to the cemento-enamel junction and to regenerate the lost periodontium. AIM: To compare the effectiveness of Platelet Rich Fibrin (PRF) and Amniotic Membrane (AM) in the treatment of gingival recession by Coronally Advanced Flap (CAF) Technique. MATERIALS AND METHODS: Thirty systemically healthy patients who met inclusion-exclusion criteria were selected...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28965367/healing-of-two-wall-intra-bony-defects-treated-with-a-novel-emd-liquid-a-pre-clinical-study-in-monkeys
#17
Yoshinori Shirakata, Richard J Miron, Yukiya Shinohara, Toshiaki Nakamura, Kotaro Sena, Naoto Horai, Dieter D Bosshardt, Kazuyuki Noguchi, Anton Sculean
AIM: To investigate the effect of a novel enamel matrix derivative formulation (EMD-liquid or Osteogain) combined with an absorbable collagen sponge (ACS) on periodontal wound healing in intra-bony defects in monkeys. MATERIALS AND METHODS: Chronic two-wall intra-bony defects were created at the distal aspect of eight teeth in three monkeys (Macaca fascicularis). The 24 defects were randomly assigned to one of the following treatments: (i) open flap debridement (OFD) + ACS alone, (ii) OFD + Emdogain + ACS (Emdogain/ACS), (iii) OFD + Osteogain + ACS (Osteogain/ACS) or (iv) OFD alone...
December 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28924388/spatiotemporal-expression-of-wnt-%C3%AE-catenin-signaling-during-morphogenesis-and-odontogenesis-of-deciduous-molar-in-miniature-pig
#18
Xiaoshan Wu, Yan Li, Fu Wang, Lei Hu, Yang Li, Jinsong Wang, Chunmei Zhang, Songlin Wang
The canonical Wnt/β-catenin signaling pathway has been shown to play essential roles in tooth initiation and early tooth development. However, the role of Wnt/β-catenin signaling in cusp patterning and crown calcification in large mammals are largely unknown. In our previous study, miniature pigs were used as the animal model due to the similarity of tooth anatomy and replacement pattern between miniature pig and human. Dynamic gene expression of third deciduous molar (DM3) in miniature pig at early stages was profiled using microarray method and expression of Wnt genes was significantly correlate with odontogenesis...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28892645/self-assembling-peptide-p-11-4-and-fluoride-for-regenerating-enamel
#19
M Alkilzy, A Tarabaih, R M Santamaria, C H Splieth
Regenerative medicine-based approaches for caries treatment focus on biomimetic remineralization of initial carious lesions as a minimal invasive therapy. In vitro, self-assembling peptide P11 -4 enhances remineralization of early carious lesions. To investigate the safety and clinical efficacy of P11 -4 for treatment of initial caries, a randomized controlled single-blind study was conducted on children aged >5 y with visible active early caries on erupting permanent molars. Subjects were randomized to either the test group (P11 -4 + fluoride varnish) or control group (fluoride varnish alone)...
February 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/28822800/amelogenin-induces-m2-macrophage-polarisation-via-pge2-camp-signalling-pathway
#20
Kensuke Yamamichi, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Yuki Nakao, Karen Yotsumoto, Hiroaki Yamato, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura
OBJECTIVES: Amelogenin, the major component of the enamel matrix derivative (EMD), has been suggested as a bioactive candidate for periodontal regeneration. Apart from producing a regenerative effect on periodontal tissues, amelogenin has also been reported to have an anti-inflammatory effect. However, the precise molecular mechanisms underlying these effects remain unclear. In the present study, we examined the immunomodulatory effects of amelogenin on macrophages. DESIGN: Human phorbol 12-myristate 13-acetate (PMA)-differentiated U937 macrophages and CD14+ peripheral blood-derived monocytes (PBMC)-derived macrophages were stimulated with recombinant amelogenin (rM180)...
August 12, 2017: Archives of Oral Biology
keyword
keyword
102377
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"