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Enamel regeneration

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https://www.readbyqxmd.com/read/29057222/identification-of-trombospondin-1-as-a-novel-amelogenin-interactor-by-functional-proteomics
#1
Angela Capolupo, Chiara Cassiano, Agostino Casapullo, Giuseppina Andreotti, Maria V Cubellis, Andrea Riccio, Raffaele Riccio, Maria C Monti
Amelogenins are a set of low molecular-weight enamel proteins belonging to a group of extracellular matrix (ECM) proteins with a key role in tooth enamel development and in other regeneration processes, such as wound healing and angiogenesis. Since only few data are actually available to unravel amelogenin mechanism of action in chronic skin healing restoration, we moved to the full characterization of the human amelogenin isoform 2 interactome in the secretome and lysate of Human Umbilical Vein Endothelial cells (HUVEC), using a functional proteomic approach...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29044527/effect-of-the-combined-use-of-enamel-matrix-derivative-and-atelocollagen-sponge-scaffold-on-osteoblastic-differentiation-of-mouse-induced-pluripotent-stem-cells-in-vitro
#2
Y Hisanaga, E Suzuki, H Aoki, M Sato, A Saito, A Saito, T Azuma
BACKGROUND AND OBJECTIVE: Induced pluripotent stem cells (iPSCs) are a candidate cell source in periodontal regenerative therapy. Enamel matrix derivative (EMD) has been shown to regenerate periodontal tissues, and atelocollagen sponge (ACS) is considered a suitable scaffold or carrier for growth factors. This study aimed to investigate the effect of combined use of EMD and an ACS scaffold on cell behaviors and differentiation of mouse iPSCs (miPSCs) in vitro. MATERIAL AND METHODS: Following embryonic body formation from miPSCs, dissociated cells (miPS-EB-derived cells) were seeded onto ACS with or without EMD, and cultured in osteoblast differentiation medium...
October 17, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29027917/porcine-dental-epithelial-cells-differentiated-in-a-cell-sheet-constructed-by-magnetic-nanotechnology
#3
Wataru Koto, Yoshinori Shinohara, Kazuyuki Kitamura, Takanori Wachi, Seicho Makihira, Kiyoshi Koyano
Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology...
October 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28969268/comparative-evaluation-of-platelet-rich-fibrin-and-dehydrated-amniotic-membrane-for-the-treatment-of-gingival-recession-a-clinical-study
#4
Ankita Jain, Gagan Rajesh Jaiswal, Kanteshwari Kumathalli, Rajesh Kumar, Alisha Singh, Aakrti Sarwan
INTRODUCTION: The main objective of any therapeutic intervention aimed at root coverage is to restore the tissue margin to the cemento-enamel junction and to regenerate the lost periodontium. AIM: To compare the effectiveness of Platelet Rich Fibrin (PRF) and Amniotic Membrane (AM) in the treatment of gingival recession by Coronally Advanced Flap (CAF) Technique. MATERIALS AND METHODS: Thirty systemically healthy patients who met inclusion-exclusion criteria were selected...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28965367/healing-of-2-wall-intrabony-defects-treated-with-a-novel-emd-liquid-a-preclinical-study-in-monkeys
#5
Yoshinori Shirakata, Richard J Miron, Yukiya Shinohara, Toshiaki Nakamura, Kotaro Sena, Naoto Horai, Dieter D Bosshardt, Kazuyuki Noguchi, Anton Sculean
AIM: To investigate the effect of a novel enamel matrix derivative formulation (EMD-liquid or Osteogain) combined with an absorbable collagen sponge (ACS) on periodontal wound healing in intrabony defects in monkeys. MATERIALS AND METHODS: Chronic 2-wall intrabony defects were created at the distal aspect of 8 teeth in 3 monkeys (Macaca fascicularis). The 24 defects were randomly assigned to one of the following treatments: (1) Open flap debridement (OFD) + ACS alone, (2) OFD + Emdogain + ACS (Emdogain/ACS), (3) OFD + Osteogain + ACS (Osteogain/ACS), or (4) OFD alone...
October 1, 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28924388/spatiotemporal-expression-of-wnt-%C3%AE-catenin-signaling-during-morphogenesis-and-odontogenesis-of-deciduous-molar-in-miniature-pig
#6
Xiaoshan Wu, Yan Li, Fu Wang, Lei Hu, Yang Li, Jinsong Wang, Chunmei Zhang, Songlin Wang
The canonical Wnt/β-catenin signaling pathway has been shown to play essential roles in tooth initiation and early tooth development. However, the role of Wnt/β-catenin signaling in cusp patterning and crown calcification in large mammals are largely unknown. In our previous study, miniature pigs were used as the animal model due to the similarity of tooth anatomy and replacement pattern between miniature pig and human. Dynamic gene expression of third deciduous molar (DM3) in miniature pig at early stages was profiled using microarray method and expression of Wnt genes was significantly correlate with odontogenesis...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28892645/self-assembling-peptide-p11-4-and-fluoride-for-regenerating-enamel
#7
M Alkilzy, A Tarabaih, R M Santamaria, C H Splieth
Regenerative medicine-based approaches for caries treatment focus on biomimetic remineralization of initial carious lesions as a minimal invasive therapy. In vitro, self-assembling peptide P11-4 enhances remineralization of early carious lesions. To investigate the safety and clinical efficacy of P11-4 for treatment of initial caries, a randomized controlled single-blind study was conducted on children aged >5 y with visible active early caries on erupting permanent molars. Subjects were randomized to either the test group (P11-4 + fluoride varnish) or control group (fluoride varnish alone)...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28822800/amelogenin-induces-m2-macrophage-polarisation-via-pge2-camp-signalling-pathway
#8
Kensuke Yamamichi, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Urara Tanaka, Yuki Nakao, Karen Yotsumoto, Hiroaki Yamato, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura
OBJECTIVES: Amelogenin, the major component of the enamel matrix derivative (EMD), has been suggested as a bioactive candidate for periodontal regeneration. Apart from producing a regenerative effect on periodontal tissues, amelogenin has also been reported to have an anti-inflammatory effect. However, the precise molecular mechanisms underlying these effects remain unclear. In the present study, we examined the immunomodulatory effects of amelogenin on macrophages. DESIGN: Human phorbol 12-myristate 13-acetate (PMA)-differentiated U937 macrophages and CD14+ peripheral blood-derived monocytes (PBMC)-derived macrophages were stimulated with recombinant amelogenin (rM180)...
August 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28773822/identification-of-specific-hydroxyapatite-001-binding-heptapeptide-by-phage-display-and-its-nucleation-effect
#9
Jing Mao, Xin Shi, Ya-Bo Wu, Shi-Qiang Gong
With recent developments of molecular biomimetics that combine genetic engineering and nanotechnology, peptides can be genetically engineered to bind specifically to inorganic components and execute the task of collagen matrix proteins. In this study, using biogenous tooth enamel as binding substrate, we identified a new heptapeptide (enamel high-affinity binding peptide, EHBP) from linear 7-mer peptide phage display library. Through the output/input affinity test, it was found that EHBP has the highest affinity to enamel with an output/input ratio of 14...
August 17, 2016: Materials
https://www.readbyqxmd.com/read/28764948/effects-of-the-incorporation-of-%C3%AE%C2%B5-aminocaproic-acid-chitosan-particles-to-fibrin-on-cementoblast-differentiation-and-cementum-regeneration
#10
Chan Ho Park, Joung-Hwan Oh, Hong-Moon Jung, Yoonnyoung Choi, Saeed Ur Rahman, Sungtae Kim, Tae-Il Kim, Hong-In Shin, Yun-Sil Lee, Frank H Yu, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Cementum formation on the exposed tooth-root surface is a critical process in periodontal regeneration. Although various therapeutic approaches have been developed, regeneration of integrated and functional periodontal complexes is still wanting. Here, we found that the OCCM30 cementoblasts cultured on fibrin matrix express substantial levels of matrix proteinases, leading to the degradation of fibrin and the apoptosis of OCCM30 cells, which was reversed upon treatment with a proteinase inhibitor, ε-aminocaproic acid (ACA)...
July 29, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28731486/demineralized-dentin-and-enamel-matrices-as-suitable-substrates-for-bone-regeneration
#11
Nina Bono, Paolo Tarsini, Gabriele Candiani
BACKGROUND: In recent decades, tooth derivatives such as dentin (D) and enamel (E) have been considered as potential graft biomaterials to treat bone defects. This study aimed to investigate the effects of demineralization on the physical-chemical and biological behavior of D and E. METHODS: Human D and E were minced into particles (Ø<1 mm), demineralized and sterilized. Thorough physical-chemical and biochemical characterizations of native and demineralized materials were performed by SEM and EDS analysis and ELISA kits to determine mineral, collagen type I and BMP-2 contents...
July 20, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28727410/exosomes-mediate-epithelium-mesenchyme-crosstalk-in-organ-development
#12
Nan Jiang, Lusai Xiang, Ling He, Guodong Yang, Jinxuan Zheng, Chenglin Wang, Yimei Zhang, Sainan Wang, Yue Zhou, Tzong-Jen Sheu, Jiaqian Wu, Kenian Chen, Paulo G Coelho, Nicky M Tovar, Shin Hye Kim, Mo Chen, Yan-Heng Zhou, Jeremy J Mao
Organ development requires complex signaling by cells in different tissues. Epithelium and mesenchyme interactions are crucial for the development of skin, hair follicles, kidney, lungs, prostate, major glands, and teeth. Despite myriad literature on cell-cell interactions and ligand-receptor binding, the roles of extracellular vesicles in epithelium-mesenchyme interactions during organogenesis are poorly understood. Here, we discovered that ∼100 nm exosomes were secreted by the epithelium and mesenchyme of a developing tooth organ and diffused through the basement membrane...
August 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28717800/from-molecules-to-macrostructures-recent-development-of-bioinspired-hard-tissue-repair
#13
REVIEW
Chunmei Ding, Zhuoxin Chen, Jianshu Li
Enamel, dentin and bone are calcified hard tissues in the human body that play significant roles in food mastication and movement support. Generally, hard tissues lack the ability of self-repair, except for the regeneration ability of bone for small-scale defects. Fabrication of man-made repair materials is therefore highly demanded. In this review, following a bioinspired strategy, we describe the composition and multiscale structures of different hard tissues, and highlight the key points for the reconstruction of hard tissues...
July 25, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28683765/periodontal-regenerative-therapy-with-enamel-matrix-derivative-in-the-treatment-of-intrabony-defects-a-prospective-2-year-study
#14
Fumi Seshima, Hideto Aoki, Takahiro Takeuchi, Eiichi Suzuki, Daisuke Irokawa, Asako Makino-Oi, Hiroki Sugito, Sachiyo Tomita, Atsushi Saito
OBJECTIVE: To date, enamel matrix derivative (EMD) has been considered to be one of the few biomaterials for clinical use capable of demonstrating true periodontal regeneration. The aim of this two-center prospective clinical study was to evaluate 2-year outcome of periodontal regenerative therapy using EMD in the treatment of intrabony defects, performed as an 'advanced medical treatment' under the national healthcare system in Japan. RESULTS: Patients with chronic periodontitis who have completed initial periodontal therapy at either of the two dental school clinics were enrolled...
July 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28644741/ras-signaling-regulates-stem-cells-and-amelogenesis-in-the-mouse-incisor
#15
X Zheng, A F Goodwin, H Tian, A H Jheon, O D Klein
The role of Ras signaling during tooth development is poorly understood. Ras proteins-which are activated by many upstream pathways, including receptor tyrosine kinase cascades-signal through multiple effectors, such as the mitogen-activated protein kinase (MAPK) and PI3K pathways. Here, we utilized the mouse incisor as a model to study how the MAPK and PI3K pathways regulate dental epithelial stem cells and amelogenesis. The rodent incisor-which grows continuously throughout the life of the animal due to the presence of epithelial and mesenchymal stem cells-provides a model for the study of ectodermal organ renewal and regeneration...
November 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28642715/elastin-like-protein-with-statherin-derived-peptide-controls-fluorapatite-formation-and-morphology
#16
Kseniya Shuturminska, Nadezda V Tarakina, Helena S Azevedo, Andrew J Bushby, Alvaro Mata, Paul Anderson, Maisoon Al-Jawad
The process of enamel biomineralization is multi-step, complex and mediated by organic molecules. The lack of cells in mature enamel leaves it unable to regenerate and hence novel ways of growing enamel-like structures are currently being investigated. Recently, elastin-like protein (ELP) with the analog N-terminal sequence of statherin (STNA15-ELP) has been used to regenerate mineralized tissue. Here, the STNA15-ELP has been mineralized in constrained and unconstrained conditions in a fluoridated solution...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28598248/enamel-matrix-derivative-enhances-the-proliferation-and-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-on-the-titanium-implant-surface
#17
Guang Li, Jing Hu, Hui Chen, Liang Chen, Na Zhang, Lisheng Zhao, Ning Wen, Yongjin Yang
Periodontal ligament stem cells (PDLSCs) have mesenchymal-stem-cells-like qualities, and are considered as one of the candidates of future clinical application in periodontal regeneration therapy. Enamel matrix derivative (EMD) is widely used in promoting periodontal regeneration. However, the effects of EMD on the proliferation and osteogenic differentiation of human PDLSCs grown on the Ti implant surface are still no clear. Therefore, this study examined the effects of EMD on human PDLSCs in vitro. Human PDLSCs were isolated from healthy participants, and seeded on the surface of Ti implant disks and stimulated with various concentrations of EMD...
July 3, 2017: Organogenesis
https://www.readbyqxmd.com/read/28566669/effect-of-enamel-matrix-derivative-on-the-angiogenic-behaviors-of-human-umbilical-vein-endothelial-cells-on-different-titanium-surfaces
#18
Bin Shi, Oleh Andrukhov, Burcu Özdemir, Hassan Ali Shokoohi Tabrizi, Michel Dard, Xiaohui Rausch-Fan
Angiogenesis play a crucial role in the regeneration of hard and soft tissue around dental titanium (Ti) implant. Enamel matrix derivative (EMD) promotes tissue regeneration and stimulates angiogenesis but its effect on the angiogenesis on Ti surfaces was never investigated. The effect of EMD on the angiogenic activity of endothelial cells cultured on pre-treated smooth Ti (PT), acidetched (A), coarse-grit blasted and acid-etched (SLA) surfaces and tissue culture plastic (TCP) in the presence or absence of EMD was investigated...
July 26, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28531879/scaffold-requirements-for-periodontal-regeneration-with-enamel-matrix-derivative-proteins
#19
Alessandra Apicella, Peggy Heunemann, Laurent Dejace, Matteo Marascio, Christopher J G Plummer, Peter Fischer
Periodontitis affects the attachment of natural teeth, and infection or inflammation associated with periodontitis may affect peri-implant tissues. Enamel matrix derivative (EMD) proteins provide stimulation for self-regeneration of the damaged tissue when applied to wide intrabony defects as part of a mixture with bone graft material. As a first step of the process enhancing cell proliferation and ligament formation, we demonstrated that EMD protein precipitation depends strongly on the physical and chemical characteristics of the bone grafts used in the mixture...
May 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28470386/human-gingival-fibroblast-response-to-enamel-matrix-derivative-porcine-recombinant-21-3-kda-amelogenin-and-5-3-kda-tyrosine-rich-amelogenin-peptide
#20
Marzena Wyganowska-Swiatkowska, Paulina Urbaniak, Daniel Lipinski, Marlena Szalata, Malgorzata Kotwicka
Enamel matrix derivative (EMD) containing a variety of protein fractions has been used for periodontal tissue regeneration. It is suggested that the proteins contained in EMD positively influence gingival fibroblasts migration and proliferation. Effects of EMD as well as of porcine recombinated 21.3-kDa amelogenin (prAMEL) and 5.3-kDa tyrosine-rich amelogenin peptide (prTRAP) on human gingival fibroblast (HGF-1, ATCC; USA) cell line were investigated. Real-time cell analysis (xCELLigence system; Roche Applied Science) was performed to determine the effects of EMD, prAMEL and prTRAP (12...
July 2017: Human Cell
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