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Enamel regeneration

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https://www.readbyqxmd.com/read/28644741/ras-signaling-regulates-stem-cells-and-amelogenesis-in-the-mouse-incisor
#1
X Zheng, A F Goodwin, H Tian, A H Jheon, O D Klein
The role of Ras signaling during tooth development is poorly understood. Ras proteins-which are activated by many upstream pathways, including receptor tyrosine kinase cascades-signal through multiple effectors, such as the mitogen-activated protein kinase (MAPK) and PI3K pathways. Here, we utilized the mouse incisor as a model to study how the MAPK and PI3K pathways regulate dental epithelial stem cells and amelogenesis. The rodent incisor-which grows continuously throughout the life of the animal due to the presence of epithelial and mesenchymal stem cells-provides a model for the study of ectodermal organ renewal and regeneration...
June 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28642715/elastin-like-protein-with-statherin-derived-peptide-controls-fluorapatite-formation-and-morphology
#2
Kseniya Shuturminska, Nadezda V Tarakina, Helena S Azevedo, Andrew J Bushby, Alvaro Mata, Paul Anderson, Maisoon Al-Jawad
The process of enamel biomineralization is multi-step, complex and mediated by organic molecules. The lack of cells in mature enamel leaves it unable to regenerate and hence novel ways of growing enamel-like structures are currently being investigated. Recently, elastin-like protein (ELP) with the analog N-terminal sequence of statherin (STNA15-ELP) has been used to regenerate mineralized tissue. Here, the STNA15-ELP has been mineralized in constrained and unconstrained conditions in a fluoridated solution...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28598248/enamel-matrix-derivative-enhances-the-proliferation-and-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-on-the-titanium-implant-surface
#3
Guang Li, Jing Hu, Hui Chen, Liang Chen, Na Zhang, Lisheng Zhao, Ning Wen, Yongjin Yang
Periodontal ligament stem cells (PDLSCs) have mesenchymal-stem-cells-like qualities, and are considered as one of the candidates of future clinical application in periodontal regeneration therapy. Enamel matrix derivative (EMD) is widely used in promoting periodontal regeneration. However, the effects of EMD on the proliferation and osteogenic differentiation of human PDLSCs grown on the Ti implant surface are still no clear. Therefore, this study examined the effects of EMD on human PDLSCs in vitro. Human PDLSCs were isolated from healthy participants, and seeded on the surface of Ti implant disks and stimulated with various concentrations of EMD...
June 9, 2017: Organogenesis
https://www.readbyqxmd.com/read/28566669/effect-of-enamel-matrix-derivative-on-the-angiogenic-behaviors-of-human-umbilical-vein-endothelial-cells-on-different-titanium-surfaces
#4
Bin Shi, Oleh Andrukhov, Burcu Özdemir, Hassan Ali Shokoohi Tabrizi, Michel Dard, Xiaohui Rausch-Fan
Angiogenesis play a crucial role in the regeneration of hard and soft tissue around dental titanium (Ti) implant. Enamel matrix derivative (EMD) promotes tissue regeneration and stimulates angiogenesis but its effect on the angiogenesis on Ti surfaces was never investigated. The effect of EMD on the angiogenic activity of endothelial cells cultured on pre-treated smooth Ti (PT), acidetched (A), coarse-grit blasted and acid-etched (SLA) surfaces and tissue culture plastic (TCP) in the presence or absence of EMD was investigated...
May 30, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28531879/scaffold-requirements-for-periodontal-regeneration-with-enamel-matrix-derivative-proteins
#5
Alessandra Apicella, Peggy Heunemann, Laurent Dejace, Matteo Marascio, Christopher J G Plummer, Peter Fischer
Periodontitis affects the attachment of natural teeth, and infection or inflammation associated with periodontitis may affect peri-implant tissues. Enamel matrix derivative (EMD) proteins provide stimulation for self-regeneration of the damaged tissue when applied to wide intrabony defects as part of a mixture with bone graft material. As a first step of the process enhancing cell proliferation and ligament formation, we demonstrated that EMD protein precipitation depends strongly on the physical and chemical characteristics of the bone grafts used in the mixture...
May 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28470386/human-gingival-fibroblast-response-to-enamel-matrix-derivative-porcine-recombinant-21-3-kda-amelogenin-and-5-3-kda-tyrosine-rich-amelogenin-peptide
#6
Marzena Wyganowska-Swiatkowska, Paulina Urbaniak, Daniel Lipinski, Marlena Szalata, Malgorzata Kotwicka
Enamel matrix derivative (EMD) containing a variety of protein fractions has been used for periodontal tissue regeneration. It is suggested that the proteins contained in EMD positively influence gingival fibroblasts migration and proliferation. Effects of EMD as well as of porcine recombinated 21.3-kDa amelogenin (prAMEL) and 5.3-kDa tyrosine-rich amelogenin peptide (prTRAP) on human gingival fibroblast (HGF-1, ATCC; USA) cell line were investigated. Real-time cell analysis (xCELLigence system; Roche Applied Science) was performed to determine the effects of EMD, prAMEL and prTRAP (12...
May 3, 2017: Human Cell
https://www.readbyqxmd.com/read/28459243/nanotechnology-in-dentistry-clinical-applications-benefits-and-hazards
#7
Govind Shashirekha, Amit Jena, Satyajit Mohapatra
Nanotechnology is emerging as an interdisciplinary field that is undergoing rapid development and has brought about enormous changes in medicine and dentistry. Nanomaterial-based design is able to mimic some of the mechanical and structural properties of native tissue and can promote biointegration. Nanotechnology has various applications in dentistry, including dentition renaturalization, therapy for dentin hypersensitivity, complete orthodontic realignment in a single visit, covalently bonding diamondized enamel, enhancing properties of root canal sealers, and continuous oral health maintenance using mechanical dentifrobots...
May 2017: Compendium of Continuing Education in Dentistry
https://www.readbyqxmd.com/read/28346861/biomimetic-remineralization-of-carious-lesions-by-self-assembling-peptide
#8
L Kind, S Stevanovic, S Wuttig, S Wimberger, J Hofer, B Müller, U Pieles
Caries is the most common disease in the world. Great efforts have been undertaken for prevention and to identify a regenerative treatment solution for dental caries. Self-assembling β-sheet forming peptides have previously shown to form 3-dimensional fiber networks supporting tissue regeneration. In particular, the self-assembling peptide P11-4 has shown potential in the treatment and prevention of dental caries. It has previously been shown that application of monomeric P11-4 solution to early carious lesions can increase net mineral gain by forming de novo hydroxyapatite crystals...
March 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28304211/furcation-therapy-with-enamel-matrix-derivative-effects-on-the-subgingival-microbiome
#9
Lucas A Queiroz, Renato C V Casarin, Shareef M Dabdoub, Dimitris N Tatakis, Enilson A Sallum, Purnima S Kumar
BACKGROUND: Although enamel matrix derivative (EMD) has been used to promote periodontal regeneration, little is known of its effect on the microbiome. Therefore, this investigation aimed to identify the changes in periodontal microbiome following treatment with EMD using a deep-sequencing approach. METHODS: Thirty-nine patients having mandibular class II buccal furcation defects were randomized to beta-tricalcium-phosphate/hydroxyapatite graft (BONE group), EMD+BONE or EMD alone...
March 17, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28287572/rapid-mix-preparation-of-bioinspired-nanoscale-hydroxyapatite-for-biomedical-applications
#10
Caroline J Wilcock, Piergiorgio Gentile, Paul V Hatton, Cheryl A Miller
Hydroxyapatite (HA) has been widely used as a medical ceramic due to its good biocompatibility and osteoconductivity. Recently there has been interest regarding the use of bioinspired nanoscale hydroxyapatite (nHA). However, biological apatite is known to be calcium-deficient and carbonate-substituted with a nanoscale platelet-like morphology. Bioinspired nHA has the potential to stimulate optimal bone tissue regeneration due to its similarity to bone and tooth enamel mineral. Many of the methods currently used to fabricate nHA both in the laboratory and commercially, involve lengthy processes and complex equipment...
February 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28261800/on-the-discovery-of-cementum
#11
REVIEW
B L Foster
Though cementum of the tooth root is critical for periodontal structure and tooth attachment and function, this tissue was not discovered and characterized on human teeth until a full century later than enamel and dentin. Early observations from the seventeenth to the nineteenth centuries by Marcello Malpighi, Antonie van Leeuwenhoek, Robert Blake, Jacques Tenon and Georges Cuvier founded a confusing and conflicting nomenclature that obscured the nature of cementum, often conflating it with bone. Advances in microscopy and histological procedures yielded the first detailed descriptions of human cementum in the 1830s by Jan Purkinje and Anders Retzius, who identified for the first time acellular and cellular types of cementum, and the resident cementocytes embedded in the latter...
March 6, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28196163/entire-papilla-preservation-technique-a-novel-surgical-approach-for-regenerative-treatment-of-deep-and-wide-intrabony-defects
#12
Serhat Aslan, Nurcan Buduneli, Pierpaolo Cortellini
Primary wound closure and uneventful early wound stability over the biomaterials are the most critical elements of successful periodontal regeneration. Yet the surgical elevation of the interdental papilla to access deep and wide intrabony defects entails an impairment of the papillary blood supply that can result in difficult healing due to a lack of primary closure in the early healing period. This negative event might complicate the healing process, favoring bacterial contamination. A novel modified tunnel surgical technique designed to maintain the integrity of the interdental papilla is presented in this article, with the aim of providing an optimal environment for wound healing in regenerative procedures...
March 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28186646/author-response
#13
LETTER
Eralp Buduneli, Nurcan Buduneli
No abstract text is available yet for this article.
June 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28167823/in-vivo-remineralization-of-dentin-using-an-agarose-hydrogel-biomimetic-mineralization-system
#14
Min Han, Quan-Li Li, Ying Cao, Hui Fang, Rong Xia, Zhi-Hong Zhang
A novel agarose hydrogel biomimetic mineralization system loaded with calcium and phosphate was used to remineralize dentin and induce the oriented densely parallel packed HA layer on defective dentin surface in vivo in a rabbit model. Firstly, the enamel of the labial surface of rabbits' incisor was removed and the dentin was exposed to oral environment. Secondly, the hydrogel biomimetic mineralization system was applied to the exposed dentin surface by using a custom tray. Finally, the teeth were extracted and evaluated by scanning electron microscopy, X-ray diffraction, and nanoindentation test after a certain time of mineralization intervals...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28149456/preventive-effects-of-different-protective-agents-on-dentin-erosion-an-in-vitro-investigation
#15
Claudio Poggio, Chiara Gulino, Maria Mirando, Marco Colombo, Giampiero Pietrocola
BACKGROUND: The purpose of this in vitro study was to evaluate the preventive effects of different protective agents on dentine erosion, measuring mean percentage weight loss. Dissolution of dentine under erosive challenges caused by soft drinks was analyzed: specimens were weighed following each immersion period, with mean percent weight losses calculated. MATERIAL AND METHODS: Extracted teeth were sectioned into uniform slabs. Seventy permanent enamel specimens were randomly distributed to seven groups...
January 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28134829/early-healing-events-after-periodontal-surgery-observations-on-soft-tissue-healing-microcirculation-and-wound-fluid-cytokine-levels
#16
Doğan Kaner, Mouaz Soudan, Han Zhao, Georg Gaßmann, Anna Schönhauser, Anton Friedmann
Early wound healing after periodontal surgery with or without enamel matrix derivative/biphasic calcium phosphate (EMD/BCP) was characterized in terms of soft tissue closure, changes of microcirculation, and expression of pro- and anti-inflammatory cytokines in gingival crevicular fluid/wound fluid (GCF/WF). Periodontal surgery was carried out in 30 patients (18 patients: application of EMD/BCP for regeneration of bony defects; 12 patients: surgical crown lengthening (SCL)). Healthy sites were observed as untreated controls...
January 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28118552/decellularized-tooth-bud-scaffolds-for-tooth-regeneration
#17
W Zhang, B Vazquez, D Oreadi, P C Yelick
Whole tooth regeneration approaches currently are limited by our inability to bioengineer full-sized, living replacement teeth. Recently, decellularized organ scaffolds have shown promise for applications in regenerative medicine by providing a natural extracellular matrix environment that promotes cell attachment and tissue-specific differentiation leading to full-sized organ regeneration. We hypothesize that decellularized tooth buds (dTBs) created from unerupted porcine tooth buds (TBs) can be used to guide reseeded dental cell differentiation to form whole bioengineered teeth, thereby providing a potential off-the-shelf scaffold for whole tooth regeneration...
May 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28117776/development-of-a-direct-pulp-capping-model-for-the-evaluation-of-pulpal-wound-healing-and-reparative-dentin-formation-in-mice
#18
Minju Song, Sol Kim, Terresa Kim, Sil Park, Ki-Hyuk Shin, Mo Kang, No-Hee Park, Reuben Kim
Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is often capped with biocompatible materials in order to expedite pulpal wound healing. The ultimate goal is to regenerate reparative dentin, a physical barrier that functions as a "biological seal" and protects the underlying pulp tissue. Although this direct pulp-capping procedure has long been used in dentistry, the underlying molecular mechanism of pulpal wound healing and reparative dentin formation is still poorly understood...
January 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28113034/biomimetic-enamel-regeneration-mediated-by-leucine-rich-amelogenin-peptide
#19
S Y Kwak, A Litman, H C Margolis, Y Yamakoshi, J P Simmer
We report here a novel biomimetic approach to the regeneration of human enamel. The approach combines the use of inorganic pyrophosphate (PPi) to control the onset and rate of enamel regeneration and the use of leucine-rich amelogenin peptide (LRAP), a nonphosphorylated 56-amino acid alternative splice product of amelogenin, to regulate the shape and orientation of growing enamel crystals. This study builds on our previous findings that show LRAP can effectively guide the formation of ordered arrays of needle-like hydroxyapatite (HA) crystals in vitro and on the known role mineralization inhibitors, like PPi, play in the regulation of mineralized tissue formation...
May 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28104393/periodontal-tissue-regeneration-using-the-cytokine-cocktail-mimicking-secretomes-in-the-conditioned-media-from-human-mesenchymal-stem-cells
#20
Kohei Sakaguchi, Wataru Katagiri, Masashi Osugi, Takamasa Kawai, Yukiko Sugimura-Wakayama, Hideharu Hibi
Secretomes in the conditioned media from human mesenchymal stem cells (MSC-CM) were previously demonstrated to promote periodontal tissue regeneration. By mixing insulin-like growth factor-1, vascular endothelial growth factor-A, and transforming growth factor-β1 which were included in MSC-CM, we made the cytokine cocktail (CC) mimicking MSC-CM, and then evaluated its efficacy on periodontal tissue regeneration. In vitro, CC promoted the migration of dog bone marrow-derived stem cells and periodontal ligament cells, and the tube formation of human umbilical vein endothelial cells...
February 26, 2017: Biochemical and Biophysical Research Communications
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