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Enamel regeneration

Kohei Sakaguchi, Wataru Katagiri, Masashi Osugi, Takamasa Kawai, Yukiko Sugimura-Wakayama, Hideharu Hibi
Secretomes in the conditioned media from human mesenchymal stem cells (MSC-CM) were previously demonstrated to promote periodontal tissue regeneration. By mixing insulin-like growth factor-1, vascular endothelial growth factor-A, and transforming growth factor-β1 which were included in MSC-CM, we made the cytokine cocktail (CC) mimicking MSC-CM, and then evaluated its efficacy on periodontal tissue regeneration. In vitro, CC promoted the migration of dog bone marrow-derived stem cells and periodontal ligament cells, and the tube formation of human umbilical vein endothelial cells...
January 16, 2017: Biochemical and Biophysical Research Communications
Md Arafat Kabir, Masaru Murata, Toshiyuki Akazawa, Kaoru Kusano, Katsuhisa Yamada, Manabu Ito
OBJECTIVES: Regenerating critical-size bone injury is a major problem that continues to inspire the design of new graft materials. Therefore, tissue engineering has become a novel approach for targeting bone regeneration applications. Human teeth are a rich source of stem cells, matrix, trace metal ions, and growth factors. A vital tooth-derived demineralized dentin matrix is acid-insoluble and composed of cross-linked collagen with growth factors. In this study, we recycled human non-functional tooth into a unique geometric dentin scaffold, entitled perforated root-demineralized dentin matrix (PR-DDM)...
January 17, 2017: Clinical Oral Implants Research
Richard J Miron, Masako Fujioka-Kobayashi, Daniel Buser, Yufeng Zhang, Dieter D Bosshardt, Anton Sculean
PURPOSE: Collagen barrier membranes were first introduced to regenerative periodontal and oral surgery to prevent fast ingrowing soft tissues (ie, epithelium and connective tissue) into the defect space. More recent attempts have aimed at combining collagen membranes with various biologics/growth factors to speed up the healing process and improve the quality of regenerated tissues. Recently, a new formulation of enamel matrix derivative in a liquid carrier system (Osteogain) has demonstrated improved physico-chemical properties for the adsorption of enamel matrix derivative to facilitate protein adsorption to biomaterials...
January 2017: International Journal of Oral & Maxillofacial Implants
Joshua D Silvertown, Bonny P Y Wong, Koneswaran S Sivagurunathan, Stephen H Abrams, Jennifer Kirkham, Bennett T Amaechi
AIM: The efficacy of self-assembling peptide P11 -4 to regenerate enamel in natural early caries lesions was evaluated over 50 days by photothermal radiometry and luminescence using The Canary System (CS) and The Canary Lab (CL). METHODS: Baseline readings for sound and carious sites on smooth surfaces of extracted teeth were obtained by scanning with CS and CL. Teeth were then randomly assigned to a treatment group (TG, treated with P11 -4), a placebo group (PG, same vehicle as treatment group without P11 -4), or a control group (CG, no treatment)...
January 4, 2017: Journal of Investigative and Clinical Dentistry
Yoshinori Shirakata, Richard J Miron, Toshiaki Nakamura, Kotaro Sena, Yukiya Shinohara, Naoto Horai, Dieter D Bosshardt, Kazuyuki Noguchi, Anton Sculean
AIM: To evaluate the effect of a novel liquid carrier system of enamel matrix derivative (Osteogain) soaked on an absorbable collagen sponge (ACS) upon periodontal wound healing/regeneration in furcation defects in monkeys. MATERIALS AND METHODS: The stability of the conventional enamel matrix derivative (Emdogain) and Osteogain adsorbed onto ACS was evaluated by ELISA. Chronic class III furcation defects were created at teeth 36, 37, 46, 47 in three monkeys (Macaca fascicularis)...
December 15, 2016: Journal of Clinical Periodontology
Tetsuya Awada, Ryo Kunimatsu, Yuki Yoshimi, Naoto Hirose, Tomomi Mitsuyoshi, Keisuke Sumi, Kotaro Tanimoto
OBJECTIVES: Amelogenins, enamel matrix proteins secreted by ameloblasts, comprise 90% of the developing extracellular enamel matrix. Recent evidence suggests that amelogenins might induce the proliferation of various cells. However, the residues comprising the active site of amelogenin remain unclear. Therefore, this study aimed to examine the effects of a human amelogenin C-terminal peptide (amgCP) on the metabolism of osteoblasts. MATERIALS AND METHODS: Mouse calvarial osteoblastic cells (MC3T3-E1) were cultured and treated with amgCP...
January 22, 2017: Biochemical and Biophysical Research Communications
Renu Devi, Jaya Dixit
INTRODUCTION: In recent years, emphasis on the use of growth factors for periodontal healing is gaining great momentum. Several growth factors showed promising results in periodontal regeneration. AIM: This study was designed to compare the clinical outcomes of 0.8μg recombinant human Vascular Endothelial Growth Factor (rh-VEGF) and 10μg recombinant human Insulin Like Growth Factor-I (rh-IGF-I) with β-Tricalcium Phosphate (β-TCP) and Polylactide-Polyglycolide Acid (PLGA) membrane in two wall intra-osseous defects...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
Carlos Parra, Y Natalie Jeong, Charles E Hawley
Recession on the lingual aspect of mandibular incisors may occur in patients with history of tongue piercing and other factitious traumatic habits. Treatment of these areas is challenging due to the site-specific anatomical features of the region. This case report presents a novel approach for a specific type of mandibular lingual defect caused by tongue piercing. A nonresorbable titanium-reinforced barrier membrane combined with an allograft and enamel matrix derivatives was used to promote regeneration of periodontal attachment...
November 2016: International Journal of Periodontics & Restorative Dentistry
Mario Aimetti, Francesco Ferrarotti, Giulia Mariani, Adriano Fratini, Marta Giraudi, Federica Romano
The present case series evaluated the potential benefits of a flapless approach in the regenerative treatment of residual deep intrabony defects. In each of 11 patients, one periodontal defect with an intrabony component of ≥ 3 mm was treated regeneratively using enamel matrix derivative combined with a closed surgical technique. Clinical and radiographic parameters were recorded at baseline and at 12 and 24 months postoperatively. All defects experienced favorable clinical and radiographic outcomes at the 24-month follow-up...
November 2016: International Journal of Periodontics & Restorative Dentistry
Amer Sehic, Amela Tulek, Cuong Khuu, Minou Nirvani, Lars Peter Sand, Tor Paaske Utheim
MicroRNAs (miRNAs) are a class of small, non-coding RNAs that provide an efficient pathway for regulation of gene expression at a post-transcriptional level. Tooth development is regulated by a complex network of cell-cell signaling during all steps of organogenesis. Most of the congenital dental defects in humans are caused by mutations in genes involved in developmental regulatory networks. Whereas the developmental morphological stages of the tooth development already are thoroughly documented, the implicated genetic network is still under investigation...
January 5, 2017: Gene
Takaaki Tsunematsu, Natsumi Fujiwara, Maki Yoshida, Yukihiro Takayama, Satoko Kujiraoka, Guangying Qi, Masae Kitagawa, Tomoyuki Kondo, Akiko Yamada, Rieko Arakaki, Mutsumi Miyauchi, Ikuko Ogawa, Yoshihiro Abiko, Hiroki Nikawa, Shinya Murakami, Takashi Takata, Naozumi Ishimaru, Yasusei Kudo
Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants of the Hertwig's epithelial root sheath (HERS) that are involved in the formation of tooth roots. ERM cells are unique epithelial cells that remain in periodontal tissues throughout adult life. They have a functional role in the repair/regeneration of cement or enamel. Here, we isolated odontogenic epithelial cells from ERM in the periodontal ligament, and the cells were spontaneously immortalized. Immortalized odontogenic epithelial (iOdE) cells had the ability to form spheroids and expressed stem cell-related genes...
October 2016: Laboratory Investigation; a Journal of Technical Methods and Pathology
Yoichi Taniguchi, Akira Aoki, Kazuto Sakai, Koji Mizutani, Walter Meinzer, Yuichi Izumi
The objective of this study was to evaluate an Er:YAG laser (ErL) application for periodontal regenerative surgery in angular bone defects at nine sites in six patients. Debridement was thoroughly performed using a combination of curettage with a Gracey-type curette and ErL irradiation at a panel setting of 70 mJ/pulse and 20 Hz with sterile saline spray. After applying an enamel matrix derivative and autogenous bone grafting, ErL was used to form a blood clot coagulation on the grafted bone surface at 50 mJ/pulse and 20 Hz without water spray for approximately 30 seconds...
July 2016: International Journal of Periodontics & Restorative Dentistry
Carlo Ghezzi, Luca Ferrantino, Luigi Bernardini, Margherita Lencioni, Silvia Masiero
The purpose of this study was to compare two minimally invasive surgical techniques (MISTs) for the treatment of periodontal defects: (1) guided tissue regeneration (GTR) using resorbable minimembrane and particulate xenograft (DBBM); and (2) inductive periodontal regeneration (IPR) using enamel matrix derivatives and DBBM. A sample of 20 infrabony periodontal defects in 20 patients were randomly assigned to either the GTR or the IPR group. A follow-up was performed at 12 months postoperative. Significant improvement in clinical parameters was observed in both groups, although no intergroup differences were found...
July 2016: International Journal of Periodontics & Restorative Dentistry
Sabine Groeger, Anita Windhorst, Joerg Meyle
BACKGROUND: In periodontal therapy enamel matrix derivative (EMD) has been successfully used for tissue regeneration by altering activity of various cells involved in periodontal regeneration. Studies have focused primarily on clinical parameters and outcome. Effects of EMD on oral epithelial cells are of crucial importance in order to understand the biology of regeneration. Aims of this study are to investigate proliferative and cytotoxic effects of EMD on oral epithelial cells and their possible influences on epithelial barrier function...
October 2016: Journal of Periodontology
R Kunimatsu, Y Yoshimi, N Hirose, T Awada, M Miyauchi, T Takata, W Li, L Zhu, P K Denbesten, K Tanimoto
BACKGROUND AND OBJECTIVES: Amelogenin proteins are the major constituent of developing extracellular enamel matrix and are believed to have an exclusively epithelial origin. Recent studies have suggested that amelogenins might induce the differentiation and maturation of various cells, including cementoblast lineage cells. However, the residues comprising the active site of amelogenin remain unclear. The purpose of this study was to identify the active site region of amelogenin by studying the effects of amelogenin fragments on the osteogenic differentiation of cementoblasts...
May 5, 2016: Journal of Periodontal Research
Ichiro Sasagawa, Shunya Oka, Masato Mikami, Hiroyuki Yokosuka, Mikio Ishiyama, Akane Imai, Hitoyata Shimokawa, Takashi Uchida
In order to compare its characteristics with those of jaw tooth collar enamel, normally developing and experimentally regenerating ganoine from ganoid scales of Lepisosteus oculatus (spotted gar), an actinopterygian fish species, was examined by Western blotting and immunohistochemistry. Amelogenin, a major enamel matrix protein (EMP), is widely found from sarcopterygian fish to mammals. Therefore, we used antimammalian amelogenin antibodies and antisera: an antibody against bovine amelogenin; antiserum against porcine amelogenin; and region-specific antibodies or antiserum against the C-terminus, middle region, or N-terminus of porcine amelogenin in this study...
May 2016: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
Mahmoud Abu-Ta'a
AIM: This randomized clinical trial compares the usefulness of adjunctive antibiotics, while strict asepsis was followed during periodontal surgery involving guided tissue regeneration. MATERIALS AND METHODS: Two groups of 20 consecutive patients each with advanced periodontal disease were randomly assigned to treatment. They displayed one angular defect each with an intrabony component ≥3 mm, probing pocket depth and probing attachment level (PAL) ≥7 mm. Test group included 13 males, mean age 60 years, treated with enamel matrix derivative (EMD) and demineralized freeze-dried bone allograft with modified papilla preservation technique, received oral amoxicillin 1 gm, 1 hour preoperatively and 2 gm for 2 days postoperatively...
January 2016: Journal of Contemporary Dental Practice
Bin Jin, Pill-Hoon Choung
Dental caries, the most prevalent oral disease in dental patients, involves the phases of demineralization and destruction of tooth hard tissues like enamel, dentin, and cementum. Dentin is a major component of the root and is also the innermost layer that protects the tooth nerve, exposure of which results in pain. In this study, we used human stem cells from apical papilla (hSCAP), which are early progenitor cells, to examine the effects of recombinant human plasminogen activator inhibitor-1 (rhPAI-1) on odontogenic differentiation in vitro and in vivo...
May 2016: Tissue Engineering. Part A
Mario Aimetti, Francesco Ferrarotti, Giulia Maria Mariani, Federica Romano
OBJECTIVES: This investigation was designed to compare the effectiveness of enamel matrix derivative (EMD) proteins in combination with flapless or flap procedure in periodontal regeneration of deep intrabony defects. MATERIALS AND METHODS: Thirty chronic periodontitis patients who had at least one residual periodontal defect with an intrabony component of ≥3 mm were consecutively enrolled. Defects were randomly assigned to test or control treatments which both consisted of the use of EMD to reach periodontal regeneration...
January 2017: Clinical Oral Investigations
Yuki Yoshimi, Ryo Kunimatsu, Naoto Hirose, Tetsuya Awada, Mutsumi Miyauchi, Takashi Takata, Wu Li, Li Zhu, Pamela Denbesten, Kazuo Tanne, Kotaro Tanimoto
BACKGROUND: Extracts of enamel matrix proteins are used to regenerate periodontal tissue; amelogenin, the most abundant enamel protein, plays an important role in this regeneration. Studies have demonstrated that amelogenin fragments promote tissue regeneration, but the bioactive site of amelogenin remains unclear. This study explores the functional domain of amelogenin by investigating effects of four amelogenin species on cementoblast proliferation. METHODS: Four amelogenin species based on amelogenin cleavage products were investigated: 1) recombinant human full-length amelogenin (rh174); 2) amelogenin cleavage product lacking the C-terminal (rh163); 3) amelogenin cleavage product lacking the N-terminal (rh128); and 4) the C-terminal region of rh174 (C11 peptide), which was synthesized and purified...
July 2016: Journal of Periodontology
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