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https://www.readbyqxmd.com/read/28945040/-ginsenoside-rh%C3%A2-induces-apoptosis-and-autophagy-of-k562-cells-by-activating-p38
#1
Xiao-Xia Liu, Jing Xia, Jia-Feng Tang, Ming-Hua Zhou, Di-Long Chen, Ze-Hong Liu
To study the effect of ginseng saponin Rh₂ in inducing apoptosis of human leukemia K562 cells, and explore its mechanism from the aspect of autophagy pathway. CCK-8 assay was used to examine the growth inhibition of human leukemia cell lines K562 treated with ginsenoside Rh₂; flow cytometry (FCM) was used to detect cell apoptosis; Hoechst staining was used to observe the changes of cell morphological apoptosis; Acridine and MDC staining were used to detect the effects of the Rh₂ on autophagy; Western blot and RT-PCR were used to detect the expression levels of the proteins closely associated with autophagy and apoptosis...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28944903/gpvi%C3%A2-fc%C3%A2-peg-improves-cerebral-infarct-volume-and-cerebral-thrombosis-in-mouse-model-with-cerebral-thrombosis
#2
Yimae Wufuer, Xuefeng Shan, Magaoweiya Sailike, Kamile Adilaimu, Songfeng Ma, Huguo Wang
Cerebral thrombosis is one of the most common causes of cerebral infarction, and anticoagulation therapy is a routine treatment in patients with hemorrhagic cerebral venous thrombosis. The hemostatic function of platelets is important for the anticoagulation therapy of thrombosis. Glycoprotein VI (GPVI) is reported as the major signaling receptor for collagen and is exclusively expressed on platelets and megakaryocytes, initiating platelet recruitment at sites of vascular injury and demonstrating numerous beneficial effects for patients with cerebral thrombosis...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944894/glucosylceramide-synthase-regulates-the-proliferation-and-apoptosis-of-liver-cells-in%C3%A2-vitro-by-bcl%C3%A2-2-bax-pathway
#3
Jun-Feng Li, Su-Jun Zheng, Li-Li Wang, Shuang Liu, Feng Ren, Yu Chen, Li Bai, Mei Liu, Zhong-Ping Duan
Our previous study found that glucosylceramide, a type of sphingolipids, was associated with liver inflammation and fibrosis. Glucosylceramide is generated by glucosylceramide synthase (GCS), which is encoded by the UDP‑glucose ceramide glucosyltransferase (UGCG) gene. GCS is a key enzyme to regulate the physiological activity of cells. However, the role of GCS in hepatic cells remains unclear. The aim of the present study was to explore the mechanism of GCS in the proliferation and apoptosis of liver cells...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944893/pigment-epithelium%C3%A2-derived-factor-protects-human-glomerular-mesangial-cells-from-diabetes-via-noxo1%C3%A2-inos-suppression
#4
Ling Gao, Yingjie Hu, Jing Li
The authors previously reported that pigment epithelium‑derived factor (PEDF) protects the diabetic kidney from fibrosis via its anti‑oxidative effects. However, the underlying molecular mechanism has never been revealed. The present study aimed to investigate how PEDF protects mesangial cells from diabetes induced damage. Human mesangial cells were exposed to a diabetic environment [30 mmol/l glucose and 200 mg advanced glycation end products (AGEs)] in the absence or presence of PEDF (200 mg/l). The superoxide and peroxynitrite productions were measured by fluorescent assay...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944824/efficient-gene-therapy-with-a-combination-of-ultrasound%C3%A2-targeted-microbubble-destruction-and-pei-dna-nls-complexes
#5
Yi-Jia Wang, Qing Zhou, Sheng Cao, Bo Hu, Qing Deng, Nan Jiang, Jingjing Cui
Current strategies of gene transfection are not efficient at achieving a notable therapeutic effect. The aim of the present study was to combine ultrasound‑targeted microbubble destruction (UTMD) with a polyethylenimine/pEGFP‑N3 plasmid/nuclear localization sequence (PEI/DNA/NLS) complex gene delivery system, and evaluate the transfection efficiency of enhanced green fluorescent protein (EGFP) gene delivery to 293T cells using this system. The formation of PEI/DNA/NLS complexes and the protective effects of PEI/NLS were verified by gel electrophoresis...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944578/impact-of-a-central-scaffold-on-the-binding-affinity-of-fragment-pairs-isolated-from-dna-encoded-self-assembling-chemical-libraries
#6
Martina Bigatti, Alberto Dal Corso, Sara Vanetti, Samuele Cazzamalli, Ulrike Rieder, Jörg Scheuermann, Dario Neri, Filippo Sladojevich
The screening of encoded self-assembling chemical libraries allows the identification of fragment pairs that bind to adjacent pockets on target proteins of interest. For practical applications, it is necessary to link these ligand pairs into discrete organic molecules, devoid of any nucleic acid component. Here we describe the discovery of a synergistic binding pair for acid alpha-1 glycoprotein and a chemical strategy for the identification of optimal linkers, connecting the two fragments. The procedure yielded a set of small organic ligands, the best of which exhibited a dissociation constant of 9...
September 24, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28944225/conserved-inhibition-of-neutrophil-extracellular-trap-release-by-clinical-candida-albicans-biofilms
#7
John F Kernien, Chad J Johnson, Jeniel E Nett
Candida albicans biofilms are difficult to eradicate due to their resistance to host defenses and antifungal drugs. Although neutrophils are the primary responder to C. albicans during invasive candidiasis, biofilms resist killing by neutrophils. Prior investigation, with the commonly used laboratory strain SC5314, linked this phenotype to the impaired release of neutrophil extracellular traps (NETs), which are structures of DNA, histones, and antimicrobial proteins involved in extracellular microbial killing...
September 2017: Journal of Fungi (Basel, Switzerland)
https://www.readbyqxmd.com/read/28944051/expanding-analytical-tools-for-characterizing-ultrasmall-silica-based-nanoparticles
#8
B Yoo, K Ma, U Wiesner, M Bradbury
C' dots are fluorescent inorganic-organic hybrid nanoparticles synthesized in water comprised of a silica core with a covalently embedded near infrared dye, and a polyethylene glycol (PEG) outer layer. C' dots containing the integrin specific ligand, cycloRGDyC, are the first of their kind particles approved for human clinical trials. In the continued clinical development of these nanoparticles, high-resolution analytical approaches are needed. Here we investigate the use of reversed phase high performance liquid chromatography (RP-HPLC) to analyze cycloRGDyC-Cy5-C' dots...
2017: RSC Advances
https://www.readbyqxmd.com/read/28943923/specific-expression-of-proton-coupled-oligopeptide-transporter-1-in-primary-hepatocarcinoma-a-novel-strategy-for-tumor-targeted-therapy
#9
Yanxia Gong, Jie Zhang, Xiang Wu, Tao Wang, Jia Zhao, Zhi Yao, Qingyu Zhang, Xi Liu, Xu Jian
Proton-coupled oligopeptide transporter 1 (PEPT1) is a membrane protein which expressed predominantly in intestine and recognized as the target of dietary nutrients (di/tripeptide) or peptidomimetic drug for delivery. The information on the existence of PEPT1 in carcinomas were limited. Our study aimed to investigate the expression profile and transport activity of PEPT1 both in human hepatocarcinoma tissues and cell lines. Western blotting and an immunofluorescence assay revealed the high level of PEPT1 protein expression in hepatocarcinoma Bel-7402, SMMC-7721, HepG2, HEP3B, SK-HEP-1 cell lines...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943902/khat-promotes-human-breast-cancer-mda-mb-231-cell-apoptosis-via-mitochondria-and-mapk-associated-pathways
#10
Yu Lu, Yanyan Li, Min Xiang, Jie Zhou, Juan Chen
Khat (Catha edulis Forsk) is a flowering evergreen plant in Eastern Africa and Southwestern Arabia. Consumption of Khat has been associated with the development of oral cancer, but its mechanism of action on the molecular level remains unclear. The present study demonstrated the cytotoxic effect of khat extracts on the human breast cancer cell line MDA-MB-231. Trypan blue exclusion assays, flow cytometry, fluorescent and electron microscopy, as well as western blotting were used to analyze the effects of Khat on the cell viability of breast cancer cells, expression of apoptotic-associated proteins and the levels of reactive oxygen species (ROS)...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943312/ubiquitin-linkage-specific-affimers-reveal-insights-into-k6-linked-ubiquitin-signaling
#11
Martin A Michel, Kirby N Swatek, Manuela K Hospenthal, David Komander
Several ubiquitin chain types have remained unstudied, mainly because tools and techniques to detect these posttranslational modifications are scarce. Linkage-specific antibodies have shaped our understanding of the roles and dynamics of polyubiquitin signals but are available for only five out of eight linkage types. We here characterize K6- and K33-linkage-specific "affimer" reagents as high-affinity ubiquitin interactors. Crystal structures of affimers bound to their cognate chain types reveal mechanisms of specificity and a K11 cross-reactivity in the K33 affimer...
September 20, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28943101/inhibition-of-transglutaminase-2-activity-increases-cisplatin-cytotoxicity-in-a-model-of-human-hepatocarcinoma-chemotherapy
#12
Dipak D Meshram, Claire V S Pike, Peter J Coussons
Transglutaminase 2 (TG2) is a ubiquitous multifunctional enzyme whose expression has been found to be altered in numerous studies of apoptosis and cell survival; its activity has been found to be increased in many types of cancer, where it is often over-expressed. Cisplatin has long been used as an effective therapeutic drug to treat numerous cancers. Although its activity is based on cross-linking of DNA, cisplatin may also operate via other mechanisms that involve modification and alteration in the activity of protein and RNA modulators of the cell cycle and apoptotic processes; these mechanisms are less well characterised...
September 21, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28942842/a-green-fluorescent-protein-based-assay-for-high-throughput-ligand-binding-studies-of-a-mycobacterial-biotin-protein-ligase
#13
Thomas E H Bond, Alanna E Sorenson, Patrick M Schaeffer
Biotin protein ligase (BirA) has been identified as an emerging drug target in Mycobacterium tuberculosis due to its essential metabolic role. Indeed, it is the only enzyme capable of covalently attaching biotin onto the biotin carboxyl carrier protein subunit of the acetyl-CoA carboxylase. Despite recent interest in this protein, there is still a gap in cost-effective high-throughput screening assays for rapid identification of mycobacterial BirA-targeting inhibitors. We present for the first time the cloning, expression, purification of mycobacterial GFP-tagged BirA and its application for the development of a high-throughput assay building on the principle of differential scanning fluorimetry of GFP-tagged proteins...
December 2017: Microbiological Research
https://www.readbyqxmd.com/read/28942285/amentoflavone-suppresses-tumor-growth-in-ovarian-cancer-by-modulating-skp2
#14
Honggai Liu, Qingfen Yue, Shehong He
AIM: Ovarian cancer is one of most common malignancies in women and is associated with high reoccurrence rate and poor prognosis. This study is designed to investigate the anti-tumor effects of amentoflavone (AF), one of the major active ingredients of S. tamariscina, against ovarian cancer. MATERIALS AND METHODS: Human ovarian cancer cell lines SKOV3 and OVCAR-3 were used in this study. The effect of AF on cell viability was examined by CCK-8 assay. Cell apoptosis and cell cycle distribution was determined by flow cytometry...
September 20, 2017: Life Sciences
https://www.readbyqxmd.com/read/28942283/edaravone-ameliorates-compression-induced-damage-in-rat-nucleus-pulposus-cells
#15
Hui Lin, Xuan Ma, Bai-Chuan Wang, Lei Zhao, Jian-Xiang Liu, Fei-Fei Pu, Yi-Qiang Hu, Hong-Zhi Hu, Zeng-Wu Shao
AIMS: Edaravone is a strong free radical scavenger most used for treating acute ischemic stroke. In this study we investigated the protective effects and underlying mechanisms of edaravone on compression-induced damage in rat nucleus pulposus (NP) cells. MATERIALS AND METHODS: Cell viability was determined using MTT assay methods. NP cell apoptosis was measured by Hoechst 33,258 staining and Annexin V/PI double staining. Intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and intracellular calcium ([Ca(2+)]i) were determined by fluorescent probes DCFH-DA, JC-1 and Fluo-3/AM, respectively...
September 20, 2017: Life Sciences
https://www.readbyqxmd.com/read/28942257/optimization-of-the-precursor-removal-of-dichloroacetonitrile-dcan-an-emerging-nitrogenous-disinfection-by-product-in-an-up-flow-bac-filter
#16
Shuai Wang, Tao Lin, Wei Chen, Han Chen
The process parameters of the up-flow biological activated carbon filter (UBACF) were optimized in a pilot-scale trial for controlling the precursor of dichloroacetonitrile (DCAN), an emerging nitrogenous disinfection by-product. The experiments were performed using a central composite design (CCD) with the response surface methodology (RSM). The results showed that the removal efficiencies of formation potentials (FP) of DCAN increased from 28.9% to 64.4% with the optimized ozone dose, expansion rate of BAC and backwashing cycle, being scheduled to 1...
September 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28942212/peptide-biosensors-for-anticancer-drugs-design-in-silico-to-work-in-denaturizing-environment
#17
Filomena Guida, Anna Battisti, Ivan Gladich, Mauro Buzzo, Elena Marangon, Luciana Giodini, Giuseppe Toffoli, Alessandro Laio, Federico Berti
One of the main targets in current clinical oncology is the development of a cheap device capable of monitoring in real-time the concentration of a drug in the blood of a patient. This would allow fine-tuning the dosage according to the patient's metabolism, a key condition to reduce side effects. By using surface plasmon resonance and fluorescence spectroscopy we here show that short peptides designed in silico by a recently developed algorithm are capable of binding the anticancer drug irinotecan (CPT-11) with micromolar affinity...
September 14, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28942142/transplantation-of-dedifferentiation-fat-cells-promotes-intervertebral-disc-regeneration-in-a-rat-intervertebral-disc-degeneration-model
#18
Enshi Nakayama, Taro Matsumoto, Tomohiko Kazama, Koichiro Kano, Yasuaki Tokuhashi
Our group has reported that mature adipocyte-derived dedifferentiated fat (DFAT) cells show multilineage differentiation potential similar to that observed in mesenchymal stem cells. In the present study, we examined whether DFAT cell transplantation could contribute to intervertebral disc regeneration using a rat intervertebral disc degeneration (IDD) model. The IDD was created in Sprague-Dawley rats by puncturing at level of caudal intervertebral disc under fluoroscopy. One week after injury, rat DFAT cells (5 × 10(4), DFAT group, n = 13) or phosphate-buffered saline (PBS, control group, n = 13) were injected into the intervertebral disc...
September 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28941789/streamlined-purification-of-fluorescently-labeled-escherichia-coli-phosphate-binding-protein-phos-suitable-for-rapid-kinetics-applications
#19
Dustin D Smith, Dylan Girodat, Hans-Joachim Wieden, L Brent Selinger
Fluorescently labeled phosphate-binding proteins can be used as biomolecular tools to measure the release of inorganic phosphate (Pi) from enzymes in real time, enabling the detailed kinetic analysis of dephosphorylating enzymes using rapid-kinetics approaches. Previously reported methods to purify fluorescently labeled phosphate-binding proteins (PhoS) from Escherichia coli are laborious, and a simplified approach is needed. Here, we report the characterization of a cytosol-localized variant (A197C) of PhoS that allows a streamlined purification for subsequent covalent conjugation with a fluorescent dye...
September 20, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28941699/synthesis-of-whey-peptide-iron-complexes-influence-of-using-different-iron-precursor-compounds
#20
Maria Elisa Caetano-Silva, Renata Carolina Alves, Guilherme Nunes Lucena, Regina Célia Galvão Frem, Maria Teresa Bertoldo-Pacheco, Juliana Azevedo Lima-Pallone, Flavia Maria Netto
Iron-binding peptides are an alternative for increasing the bioavailability of iron and to decreasing its pro-oxidant effect. This study aimed to synthesize and characterize peptide-iron complexes using FeCl2 or FeSO4 as the iron precursor compounds. Whey protein isolate (WPI), WPI hydrolyzed with pancreatin, and its fractions obtained via ultrafiltration (cut-off 5kDa) were used as ligands. The fluorescence intensity of the ligands significantly decreased as the iron concentration increased as a result of metal coordination with the iron-binding sites, which may have led to changes in the microenvironment of tryptophan...
November 2017: Food Research International
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