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https://www.readbyqxmd.com/read/29777949/evaluation-of-skin-or-sweat-characteristic-mrnas-for-inferring-the-human-origin-of-touched-contact-traces
#1
Tomoko Akutsu, Ken Watanabe, Ayari Takamura, Koichi Sakurada
The source of small amounts of touch DNA, which is transferred from the skin to an object when it is handled or touched, could be an issue in the forensic analysis of criminal cases. Here, we performed an extended evaluation of skin- or sweat-characteristic mRNAs to investigate their usability to infer whether an object has been handled or touched by someone. First, we compared the expression levels of candidate genes between skin swabs and other body fluids by quantitative RT-PCR analysis. Among the analyzed genes, corneodesmosin (CDSN), late cornified envelope 1C (LCE1C), filaggrin (FLG), desmocollin 1, and dermcidin were selected for further analysis on the basis of their specificities and sensitivities...
May 14, 2018: Legal Medicine
https://www.readbyqxmd.com/read/29777694/gapdh-targeted-therapy-a-new-approach-for-secondary-damage-after-traumatic-brain-injury-on-rats
#2
Vladimir F Lazarev, Elizaveta A Dutysheva, Elena Y Komarova, Elena R Mikhaylova, Irina V Guzhova, Boris A Margulis
Massive neuronal death caused by a neurodegenerative pathology or damage due to ischaemia or traumatic brain injury leads to the appearance of cytosolic proteins in the extracellular space. We found that one of the most abundant cellular polypeptides, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), appearing in the medium of dying cells or body fluids is able to form aggregates that are cytotoxic to adjacent cells. Since we previously showed that the hydrocortisone derivative RX624 can inhibit the ability of GAPDH to transport the enzyme complex with polyglutamine and reduce the cytotoxicity of the complex, we explored the effects of GAPDH on SH-SY5Y neuroblastoma cells...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777288/time-shifting-effects-of-methylphenidate-on-daily-rhythms-in-the-diurnal-rodent-arvicanthis-ansorgei
#3
Jorge Mendoza, Hester C van Diepen, Rob Rodrigues Pereira, Johanna H Meijer
People suffering of attention-deficit/hyperactivity disorder (ADHD) and treated with the psychostimulant methylphenidate (MPH) show sleep-wake cycle and daily rhythm alterations despite the beneficial effects of MPH on behavioral symptoms (i.e., hyperactivity, attention). In nocturnal rodents (i.e., mice), chronic exposure to MPH alters the neural activity of the circadian clock in the suprachiasmatic nucleus (SCN), behavioral rhythms, and the sleep-wake cycle. Here, we studied the effects of MPH on daily rhythms of behavior and body temperature of the diurnal rodent Arvicanthis ansorgei...
May 18, 2018: Psychopharmacology
https://www.readbyqxmd.com/read/29777120/softness-induced-enhancement-in-net-throughput-of-non-linear-bio-fluids-in-nanofluidic-channel-under-edl-phenomenon
#4
Harshad Sanjay Gaikwad, Pranab Kumar Mondal, Somchai Wongwises
In this article, we describe the electro-hydrodynamics of non-Newtonian fluid in narrow fluidic channel with solvent permeable and ion-penetrable polyelectrolyte layer (PEL) grafted on channel surface with an interaction of non-overlapping electric double layer (EDL) phenomenon. In this analysis, we integrate power-law model in the momentum equation for describing the non-Newtonian rheology. The complex interplay between the non-Newtonian rheology and interfacial electrochemistry in presence of PEL on the walls leads to non-intuitive variations in the underlying flow dynamics in the channels...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29776162/voronoi-glass-forming-liquids-a-structural-study
#5
C Ruscher, J Baschnagel, J Farago
We introduce a theoretical model of simple fluid, whose interactions, defined in terms of the Voronoi cells of the configurations, are local and many-body. The resulting system is studied both theoretically and numerically. We show that the fluid, though sharing the global features of other models of fluids with soft interactions, has several unusual characteristics, which are investigated and discussed.
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29775178/absence-of-nosocomial-transmission-of-imported-lassa-fever-during-use-of-standard-barrier-nursing-methods
#6
Anna Grahn, Andreas Bråve, Thomas Tolfvenstam, Marie Studahl
Nosocomial transmission of Lassa virus (LASV) is reported to be low when care for the index patient includes proper barrier nursing methods. We investigated whether asymptomatic LASV infection occurred in healthcare workers who used standard barrier nursing methods during the first 15 days of caring for a patient with Lassa fever in Sweden. Of 76 persons who were defined as having been potentially exposed to LASV, 53 provided blood samples for detection of LASV IgG. These persons also responded to a detailed questionnaire to evaluate exposure to different body fluids from the index patient...
June 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29774938/-analysis-of-risk-factors-of-prolonged-postoperative-ileus-after-gastric-cancer-surgery
#7
Ning Wang, Lin Chen, Mingsen Li, Naizhong Jin, Bo Wei
OBJECTIVE: To investigate the risk factors of prolonged postoperative ileus (PPOI) after gastric cancer surgery. METHODS: Definition of PPOI was that gastrointestinal function did not return to normal within 96 hours after operation. Diagnostic criteria of PPOI were as follows: according with over 2 below conditions at postoperative 97-hour: (1) moderate to severe sick (mild: 1-3 points, moderate: 4-7 points, severe: 8-10 points) or vomiting occurred in past 12 hours...
May 25, 2018: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/29773182/essential-elements-in-synovial-fluid-samples-obtained-from-patients-living-in-northern-poland
#8
Pawel Konieczynski, Grzegorz Szreder, Ewelina Tamowska, Marek Wesolowski
Many people after 50 year of life suffer pains caused by osteoarthritis (OA), which make their life difficult and painful. There is a probable association of OA with the concentration of certain elements in body fluids. Therefore the aim of the study was to relate the level of essential elements in synovial fluids of patients with OA with the treatment using antiarthritic drugs. Thus, flame-atomic absorption spectrometry was used to determine the concentrations of Fe, Zn, Cu, Mn, Na, K, Ca and Mg in 74 samples of synovial fluids taken from 36 patients...
July 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29772856/-application-of-broad-easy-immediate-surgery-in-percutaneous-transforaminal-endoscopic-technology-for-lumbar-lateral-recess-stenosis-in-the-elderly
#9
Shi-Min Zhang, Guan-Nan Wu, Jiao Jin, Yu-Zhang Liu, Zuo-Xu Li, Zhao-Jie Zhang, Ming Ma, Tao Chong, Yong-Dong Zhang
OBJECTIVE: To explore the safety and effectiveness of percutaneous transforaminal endoscopic BEIS technology for lumbar lateral recess stenosis in the elderly. METHODS: From February 2014 to May 2016, 21 patients with lumbar lateral recess stenosis in elderly were treated with percutaneous endoscopic BEIS. There were 13 males and 8 females, aged from 70 to 85 years old with an average of 74.3 years. Preoperative, 1 and 12 months postoperative visual analogue scale(VAS) scores and Oswestry Disability Index(ODI) were statistically analyzed...
April 25, 2018: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29772473/electrophoretic-co-deposition-of-peek-hydroxyapatite-composite-coatings-for-biomedical-applications
#10
Fatih E Baştan, Muhammad Atiq Ur Rehman, Yasemin Yıldıran Avcu, Egemen Avcu, Fatih Üstel, Aldo R Boccaccini
This study focuses on the optimization of electrophoretic deposition (EPD) and suspension parameters for producing PEEK-hydroxyapatite (HA) coatings with feasible microstructure, adhesion strength, and in-vitro bioactivity. Nanostructured hydroxyapatite (HA) micro-granules were incorporated with PEEK to form PEEK-hydroxyapatite composite coatings via EPD. After EPD, a heat-treatment at 375 °C was applied for densification of the coatings and for enhancing the adhesion between the coatings and the substrates...
May 3, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29771707/electrolytes-and-biochemical-changes-in-cerebrospinal-fluid-in-drowning-experimental-rabbit-model
#11
Melad G Paulis, Eman I Hasan
The diagnosis of drowning is still a difficult task in forensic science. Biochemical changes in different body fluids have been examined for the identification of drowning. However, none of them alone gives accurate results in the diagnosis of drowning and differentiation of saltwater and freshwater drowning. This study aimed to examine cerebrospinal fluid changes in drowned rabbits. Six groups of rabbits were used including immersed dead rabbits in freshwater or saltwater (as control groups), alive fully conscious rabbits drowned in freshwater and saltwater, and anesthetized rabbits drowned in freshwater and saltwater...
May 15, 2018: American Journal of Forensic Medicine and Pathology
https://www.readbyqxmd.com/read/29771636/subcellular-topography-modulates-actin-dynamics-and-signaling-in-b-cells
#12
Christina M Ketchum, Xiaoyu Sun, Alexandra Suberi, John T Fourkas, Wenxia Song, Arpita Upadhyaya
B cell signaling activation is most effectively triggered by the binding of B cell receptors (BCRs) to membrane-bound antigens.  In vivo, B cells encounter antigen on antigen presenting cells (APC), which possess complex surfaces with convoluted topographies, a fluid membrane and deformable cell bodies. However, whether and how the physical properties of antigen presentation affect B cell activation is not well understood. Here, we use nanotopographic surfaces that allow systematic variation of geometric parameters to show that surface features on a subcellular scale influence B cell signaling and actin dynamics...
May 17, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29771528/host-cell-prediction-of-exosomes-using-morphological-features-on-solid-surfaces-analyzed-by-machine-learning
#13
Kazuki Ito, Yuta Ogawa, Keiji Yokota, Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Kiyotaka Shiba, Yasuo Kimura, Ayumi Hirano-Iwata, Yuzuru Takamura, Toshio Ogino
Exosomes are extracellular nanovesicles released from any cells and found in any body-fluid. Because exosomes exhibit information of their host cells (secreting cells), their analysis is expected to be a powerful tool for early diagnosis of cancers. To predict the host cells, we extracted multi-dimensional feature data about size, shape, and deformation of exosomes immobilized on solid surfaces by atomic force microscopy (AFM). The key idea is combination of support vector machine (SVM) learning for individual exosome particles and their interpretation by principal component analysis (PCA)...
May 17, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29767114/effects-of-physical-form-of-diet-on-nutrient-digestibility-rumen-fermentation-rumination-growth-performance-and-protozoa-population-of-finishing-lambs
#14
Elham Karimizadeh, Morteza Chaji, Tahereh Mohammadabadi
This study was conducted to compare effects of 3 physical forms of feed including mash (diet 1), pellet (diet 2) and complete feed block (CFB; diet 3) on digestion, fermentation and performance of lambs. Twenty-one lambs with an initial average body weight of 26 ± 2.5 kg and 6 ± 1.5 months of age were assigned through a completely randomized design to 3 treatments and 7 replicates. The experimental treatments had the same formulation. The results of present experiment showed that CFB significantly increased feed intake and nutrient digestibility ( P  < 0...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766608/effects-of-supplementary-desalted-mother-liquor-as-replacement-of-commercial-salt-in-diet-for-thai-native-cattle-on-digestibility-energy-and-nitrogen-balance-and-rumen-conditions
#15
Yoshiaki Sato, Wanna Angthong, Patima Butcha, Motoharu Takeda, Kazato Oishi, Hiroyuki Hirooka, Hajime Kumagai
Four Thai native cattle were used in a 4 × 4 Latin square design experiment to evaluate the availability of desalted mother liquor (DML) as replacement of salt in concentrate. Each cattle was assigned to one of the following concentrate feeding treatments: C1, 1% NaCl was added as salt; C2, 2% NaCl was added as salt; D1, 1% NaCl was replaced by DML; D2, 2% NaCl was replaced by DML, on a dry matter (DM) basis. The animals were fed rice straw and experimental concentrates (40:60) at 1.9% of body weight on a DM basis, daily...
May 16, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29765575/association-of-serum-testosterone-and-dehydroepiandrosterone-sulfate-with-rheumatoid-arthritis-a-case-control-study
#16
Mahin Lashkari, Akram Noori, Sonia Oveisi, Mohammadreza Kheirkhah
Background: It is supposed that hypoandrogenism may be involved in the pathogenesis of rheumatoid arthritis (RA). Testosterone and dehydroepiandrosterone sulfate (DHEAs) levels decrease in body fluids of patients with RA. Objective: The aim of this study was to determine the association of serum testosterone and DHEAs with RA. Methods: This case-control study was conducted on 59 patients with RA and 61 healthy gender- and age-matched controls at Qazvin University of Medical Sciences, Qazvin, Iran, in 2014...
March 2018: Electronic Physician
https://www.readbyqxmd.com/read/29764386/parkinson-s-disease-diagnostic-observations-paddo-study-rationale-and-design-of-a-prospective-cohort-study-for-early-differentiation-of-parkinsonism
#17
Anouke van Rumund, Marjolein B Aerts, Rianne A J Esselink, Frederick J A Meijer, Marcel M Verbeek, Bastiaan R Bloem
BACKGROUND: Differentiation of Parkinson's disease (PD) from the various types of atypical parkinsonism (AP) such as multiple system atrophy (MSA), progressive supranuclear palsy (PSP), dementia with Lewy bodies (DLB), corticobasal syndrome (CBS) and vascular parkinsonism (VP), can be challenging, especially early in the disease course when symptoms overlap. A major unmet need in the diagnostic workup of these disorders is a diagnostic tool that differentiates the various disorders, preferably in the earliest disease stages when the clinical presentation is similar...
May 16, 2018: BMC Neurology
https://www.readbyqxmd.com/read/29763447/hepatitis-b-and-c-seroprevalence-knowledge-practice-and-associated-factors-among-medicine-and-health-science-students-in-northeast-ethiopia
#18
Wondmagegn Demsiss, Abdurahaman Seid, Temesgen Fiseha
BACKGROUND: Health care professionals, especially medical students, are at greater risk of contracting hepatitis B and C virus infections due to their occupational exposure to percutaneous injuries and other body fluids. The aim of this study was to determine the seroprevalence of hepatitis B and C virus infections among medicine and health science students in Northeast Ethiopia and to assess their knowledge and practice towards the occupational risk of viral hepatitis. METHODS: A cross-sectional study was conducted among a total of 408 medicine and health science students during the period from March to September 2017...
2018: PloS One
https://www.readbyqxmd.com/read/29762821/the-influencing-mechanism-of-mtor-signal-pathway-mediated-by-mitofusin-2-in-development-of-follicle
#19
Q-Y Luo, P Huo, L-L Wang, X-H Wu
OBJECTIVE: To investigate the influencing mechanism of mammalian target of rapamycin (mTOR) signal pathway mediated by mitofusin-2 (Mfn2) in the development of follicle. MATERIALS AND METHODS: We selected 20 healthy Sprague Dawley (SD) female rats aging between 6 and 8 weeks were divided into the control group and the polycystic ovarian syndrome (PCOS) model group. Rats in PCOS group received the lavage using 0.4 mL 1% carboxymethyl cellulose solution containing letrozole (1 mg/kg/d) consecutively for 20 to 25d...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29761349/intracochlear-administration-of-steroids-with-a-catheter-during-human-cochlear-implantation-a-safety-and-feasibility-study
#20
Nils K Prenzler, Rolf Salcher, Max Timm, Lutz Gaertner, Thomas Lenarz, Athanasia Warnecke
Suppression of foreign body reaction, improvement of electrode-nerve interaction, and preservation of residual hearing are essential research topics in cochlear implantation. Intracochlear pharmaco- or cell-based therapies can open new horizons in this field. Local drug delivery strategies are desirable as higher local concentrations of agents can be realized and side effects can be minimized compared to systemic administrations. When administered locally at accessible, basal parts of the cochlea, drugs reach apical regions later and in much lower concentrations due to poor diffusion patterns in cochlear fluids...
May 14, 2018: Drug Delivery and Translational Research
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