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https://www.readbyqxmd.com/read/29332274/callitriche-cophocarpa-water-starwort-proteome-under-chromate-stress-evidence-for-induction-of-a-quinone-reductase
#1
Paweł Kaszycki, Aleksandra Dubicka-Lisowska, Joanna Augustynowicz, Barbara Piwowarczyk, Wojciech Wesołowski
Chromate-induced physiological stress in a water-submerged macrophyte Callitriche cophocarpa Sendtn. (water starwort) was tested at the proteomic level. The oxidative stress status of the plant treated with 1 mM Cr(VI) for 3 days revealed stimulation of peroxidases whereas catalase and superoxide dismutase activities were similar to the control levels. Employing two-dimensional electrophoresis, comparative proteomics enabled to detect five differentiating proteins subjected to identification with mass spectrometry followed by an NCBI database search...
January 13, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29332140/identification-of-cross-reactive-antigens-of-c-botulinum-types-a-b-e-f-by-immunoproteomic-approach
#2
Arti Sharma, Sarkaraisamy Ponmariappan, Rani Sarita, Syed Imtiaz Alam, Dev Vrat Kamboj, Sangeeta Shukla
Diseases triggered by microorganisms can be controlled by vaccines, which need neutralizing antigens. Hence, it is very crucial to identify extremely efficient immunogens for immune prevention. Botulism, a fatal neuroparalytic disease, is caused by botulinum neurotoxins produced by the anaerobic, Gram-positive spore-forming bacteria, Clostridium botulinum. Food-borne botulism and iatrogenic botulism are caused by botulinum toxin. Wound botulism, infant botulism, and adult intestinal botulism are caused by primarily C...
January 13, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29330702/ptac10-an-s1-domain-containing-component-of-the-transcriptionally-active-chromosome-complex-is-essential-for-plastid-gene-expression-in-arabidopsis-thaliana-and-is-phosphorylated-by-chloroplast-targeted-casein-kinase-ii
#3
Qing-Bo Yu, Tuan-Tuan Zhao, Lin-Shan Ye, Ling Cheng, Ying-Qian Wu, Chao Huang, Zhong-Nan Yang
In higher plant chloroplasts, the plastid-encoded RNA polymerase (PEP) consists of four catalytic subunits and numerous nuclear-encoded accessory proteins, including pTAC10, an S1-domain-containing protein. In this study, pTAC10 knockout lines were characterized. Two ptac10 mutants had an albino phenotype and severely impaired chloroplast development. The pTAC10 genomic sequence fused to a four-tandem MYC tag driven by its own promoter functionally complemented the ptac10-1 mutant phenotype. pTAC10 was present in both the chloroplast stroma and thylakoids...
January 12, 2018: Photosynthesis Research
https://www.readbyqxmd.com/read/29329271/transketolase-is-identified-as-a-target-of-herbicidal-substance-%C3%AE-terthienyl-by-proteomics
#4
Bin Zhao, Jingqian Huo, Ning Liu, Jinlin Zhang, Jingao Dong
α-terthienyl is a natural phytotoxin isolated originally from Flaveria bidentis (L.) Kuntze. The bioassay presented here shows the strong herbicidal activity of α-terthienyl on Digitaria sanguinalis, Arabidopsis thaliana and Chlamydomonas reinhardtii. The α-terthienyl-induced response of A. thaliana at the protein level was analyzed at different times. Changes in the protein expression profiles were analyzed by two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry (LC-MS/MS) mass spectrometry...
January 12, 2018: Toxins
https://www.readbyqxmd.com/read/29329085/an-integrated-approach-to-assess-the-impacts-of-zinc-pyrithione-at-different-levels-of-biological-organization-in-marine-mussels
#5
Lorna J Dallas, Andrew Turner, Tim P Bean, Brett P Lyons, Awadhesh N Jha
The mechanisms of sublethal toxicity of the antifouling biocide, zinc pyrithione (ZnPT), have not been well-studied. This investigation demonstrates that 14-d sublethal exposure to ZnPT (0.2 or 2 μM, alongside inorganic Zn and sea water controls) is genotoxic to mussel haemocytes but suggests that this is not caused by oxidative DNA damage as no significant induction of oxidised purines was detected by Fpg-modified comet assay. More ecologically relevant endpoints, including decreased clearance rate (CR), cessation of attachment and decreased tolerance of stress on stress (SoS), also showed significant response to ZnPT exposure...
December 27, 2017: Chemosphere
https://www.readbyqxmd.com/read/29329019/salidroside-influences-the-cellular-cross-talk-of-human-fetal-lung-diploid-fibroblasts-a-proteomic-approach
#6
Wenmin Xing, Wenyan Gao, Huili Su, Sanying Wang, Jing Zhang, Genxiang Mao, Jing Yan
Senescence is a complex multiple factor proces, which is still poorly understood. The purpose of this study was to find the proteome of cultured human fetal lung diploid fibroblasts (2BS) of different population doubling (PD), as well as the altered proteome induced by salidroside (SAL) in 2BS cells. Proteins were identified by two-dimensional electrophoresis (2-DE) combining matrix-assisted laser desorption/ionization-time and flight mass spectrometry (MAL DI-TOF/MS). As a result, we found 16 proteins with two-fold variations in senescent cells or after SAL treatment, some being reduced such as reticulocalbin-1, heat shock protein beta-6, elongation factor 1-delta, F-actin-capping protein subunit alpha-1, and chloride intracellular channel 1...
January 5, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29327416/comparative-proteomics-studies-of-insect-cuticle-by-tandem-mass-spectrometry-application-of-a-novel-proteomics-approach-to-the-pea-aphid-cuticular-proteins
#7
Masson Victor, Arafah Karim, Voisin Sébastien, Bulet Philippe
The exoskeleton of arthropods, called cuticle, is a biological composite material consisting principally of N-acetylglucosamine polymer embedded in cuticular proteins (CPs). The CPs have been studied and characterized by mass spectrometry in several cuticular structures and in many arthropods. Such analyses were usually carried out by protein extraction using SDS detergent followed by electrophoresis, allowing detection and identification of a large number of CPs. To build a repertoire of cuticular structures from Bombyx mori, Apis mellifera and Anopheles gambiae we avoided the use of SDS and electrophoresis...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29324245/heavy-light-chain-assay-as-a-biomarker-for-diagnosis-and-follow-up-of-multiple-myeloma
#8
Hyojin Chae, Eunhee Han, Jaeeun Yoo, Jaewoong Lee, Jeong Joong Lee, Kyoungho Cha, Myungshin Kim, Yonggoo Kim, Sung-Eun Lee, Chang-Ki Min
BACKGROUND: The heavy-light chain (HLC) assay enables the accurate measurement of each isotype-specific heavy and light chain (i.e., IgGĸ, IgGλ, IgAĸ, and IgAλ) and the derivation of an HLC-pair ratio. However, to date, only limited data have validated the usefulness of serial HLC measurements in the routine follow-up of intact immunoglobulin multiple myeloma (MM) patients. METHODS: A total of 36 diagnostic and 671 post-treatment sera from 115 IgG and 61 IgA MM patients were assessed with capillary zone electrophoresis, immunosubtraction electrophoresis, total immunoglobulin measurement, free light chain, and HLC assay...
January 8, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29323472/integrated-analysis-of-the-transcriptome-and-metabolome-of-corynebacterium-glutamicum-during-penicillin-induced-glutamic-acid-production
#9
Takashi Hirasawa, Masaki Saito, Katsunori Yoshikawa, Chikara Furusawa, Hiroshi Shmizu
Corynebacterium glutamicum is known for its ability to produce glutamic acid and has been utilized for the fermentative production of various amino acids. Glutamic acid production in C. glutamicum is induced by penicillin. In this study, we analyzed the transcriptome and metabolome of C. glutamicum to understand the mechanism of penicillin-induced glutamic acid production. Transcriptomic analysis with DNA microarray revealed that expression of some glycolysis- and TCA cycle-related genes, which include those encoding the enzymes involved in conversion of glucose to 2-oxoglutaric acid, was upregulated after penicillin addition...
January 11, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29323083/monoclonal-gammopathy-with-double-m-bands-an-atypical-presentation-on-serum-protein-electrophoresis-simulating-biclonal-gammopathy
#10
Kaustubh Bora, Umesh Das, Bhupen Barman, Alice Abraham Ruram
Monoclonal gammopathies, such as multiple myeloma, typically exhibit high levels of a monoclonal immunoglobulin (M-protein), produced by a clone of abnormally proliferating B-lymphocytes and/or plasma cells. The M-protein can be evaluated by serum protein electrophoresis (SPEP), which yields a single discrete band (M-band), usually in the γ-globulin region. Rarely, two M-bands appear simultaneously at different positions during SPEP - a condition known as biclonal gammopathy, which is a result of clonal expansion of two different neoplastic cell lines...
October 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29322369/proteomic-and-metabolomic-analysis-of-marine-medaka-oryzias-melastigma-after-acute-ammonia-exposure
#11
Limei Zhu, Na Gao, Ruifang Wang, Li Zhang
Ammonia is both a highly toxic environmental pollutant and the major nitrogenous waste produced by ammoniotelic teleosts. Although the acute toxic effects of ammonia have been widely studied in fish, the biochemical mechanisms of its toxicity have not been understood comprehensively. In this study, we performed comparative proteomic and metabolomic analysis between ammonia-challenged (1.2 and 2.6 mmol L-1 NH4Cl for 96 h) and control groups of marine medaka (Oryzias melastigma) to identify changes of the metabolite and protein profiles in response to ammonia stress...
January 10, 2018: Ecotoxicology
https://www.readbyqxmd.com/read/29320819/molecular-and-biochemical-characterization-of-opisthorchis-viverrini-calreticulin
#12
Wanlapa Chaibangyang, Amornrat Geadkaew-Krenc, Suksiri Vichasri-Grams, Smarn Tesana, Rudi Grams
Calreticulin (CALR), a multifunctional protein thoroughly researched in mammals, comprises N-, P-, and C-domain and has roles in calcium homeostasis, chaperoning, clearance of apoptotic cells, cell adhesion, and also angiogenesis. In this study, the spatial and temporal expression patterns of the Opisthorchis viverrini CALR gene were analyzed, and calcium-binding and chaperoning properties of recombinant O. viverrini CALR (OvCALR) investigated. OvCALR mRNA was detected from the newly excysted juvenile to the mature parasite by RT-PCR while specific antibodies showed a wide distribution of the protein...
December 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29317173/identification-of-a-proteomic-biomarker-associated-with-invasive-st1-serotype-vi-group-b-streptococcus-by-maldi-tof-ms
#13
Hsiao-Chuan Lin, Jang-Jih Lu, Lee-Chung Lin, Cheng-Mao Ho, Kao-Pin Hwang, Yu-Ching Liu, Chao-Jung Chen
BACKGROUND: Group B Streptococcus (GBS) is an important invasive pathogen in neonates, pregnant women and the elderly. Serotype VI GBS, which has been rarely reported globally, has emerged as a significant pathogen in Asia. However, traditional serologic latex agglutination (LA) methods may fail to type isolates that lack of or low expression of CPS. METHODS: A total of 104 GBS strains were analyzed by MALDI-TOF MS. Multiplex PCR and multilocus sequence typing (MLST) were also performed to confirm their strains...
December 30, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29313518/transferrin-and-lactoferrin-human-iron-sources-for-enterococci
#14
Paweł Lisiecki
To overcome limitations in iron acquisition, enterococci have evolved a number of mechanisms to scavenge iron from the host iron-binding proteins - transferrin (TR) and lactoferrin (LF). The aim of this study was to demonstrate the mechanisms by which enterococci utilize human TR and LF bound iron. The study included two strains of Enterococcus faecalis grown in iron-deficient and iron-excess media respectively. The binding activity of both proteins was monitored using proteins labelled with 125I. The uptake of iron by enterococci was determined using 59Fe labelled proteins...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29312627/a-proteomic-approach-for-the-identification-of-biomarkers-in-endometrial-cancer-uterine-aspirate
#15
Blendi Ura, Lorenzo Monasta, Giorgio Arrigoni, Cinzia Franchin, Oriano Radillo, Isabel Peterlunger, Giuseppe Ricci, Federica Scrimin
Endometrial cancer arises from the endometrium. It has a slow progression and a reported survival rate of 75%. The identification of soluble biomarkers in the uterine aspirate may be very useful for its early diagnosis. Uterine aspirates from 10 patients with endometrial cancer and 6 non-endometrial cancer controls were analyzed by two-dimensional gel electrophoresis coupled with mass spectrometry and western blotting for data verification. A total of 25 proteins with fold change in %V ≥2 or ≤0.5 in intensity were observed to change significantly (P<0...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29311229/integrated-analysis-of-proteomic-and-transcriptomic-data-highlights-late-fetal-muscle-maturation-process
#16
Valentin Voillet, Magali San Cristobal, Marie-Christine Pere, Yvon Billon, Laurianne Canario, Laurence Liaubet, Louis Lefaucheur
BACKGROUND: In pigs, the perinatal period is the most critical time for survival. Piglet maturation, which occurs at the end of gestation, leads to a state of full development after birth. Maturity is thus an important determinant of early survival. Skeletal muscle plays a key role in adaptation to extra-uterine life, e.g. motor function and thermoregulation. Progeny from two breeds with extreme neonatal mortality rates were analyzed at 90 and 110 days of gestation (dg). The Large White breed is a highly selected breed for lean growth with a high rate of mortality at birth, whereas the Chinese Meishan breed is fatter and more robust and has a low mortality rate...
January 8, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29310793/methods-for-the-visualization-and-analysis-of-extracellular-matrix-protein-structure-and-degradation
#17
Annemarie K Leonard, Elizabeth A Loughran, Yuliya Klymenko, Yueying Liu, Oleg Kim, Marwa Asem, Kevin McAbee, Matthew J Ravosa, M Sharon Stack
This chapter highlights methods for visualization and analysis of extracellular matrix (ECM) proteins, with particular emphasis on collagen type I, the most abundant protein in mammals. Protocols described range from advanced imaging of complex in vivo matrices to simple biochemical analysis of individual ECM proteins. The first section of this chapter describes common methods to image ECM components and includes protocols for second harmonic generation, scanning electron microscopy, and several histological methods of ECM localization and degradation analysis, including immunohistochemistry, Trichrome staining, and in situ zymography...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29310280/two-dimensional-chromatographic-analysis-using-three-second-dimension-columns-for-continuous-comprehensive-analysis-of-intact-proteins
#18
Zaifang Zhu, Huang Chen, Jiangtao Ren, Juan J Lu, Congying Gu, Kyle B Lynch, Si Wu, Zhe Wang, Chengxi Cao, Shaorong Liu
We develop a new two-dimensional (2D) high performance liquid chromatography (HPLC) approach for intact protein analysis. Development of 2D HPLC has a bottleneck problem - limited second-dimension (second-D) separation speed. We solve this problem by incorporating multiple second-D columns to allow several second-D separations to be proceeded in parallel. To demonstrate the feasibility of using this approach for comprehensive protein analysis, we select ion-exchange chromatography as the first-dimension and reverse-phase chromatography as the second-D...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310225/sensitive-and-fast-characterization-of-site-specific-protein-glycosylation-with-capillary-electrophoresis-coupled-to-mass-spectrometry
#19
Yanyan Qu, Liangliang Sun, Guijie Zhu, Zhenbin Zhang, Elizabeth H Peuchen, Norman J Dovichi
Glycoproteomic analysis requires efficient separation and sensitive detection to enable the comprehensive characterization of glycan heterogeneity. Here, we report the use of capillary zone electrophoresis-electrospray ionization-mass spectrometry (CZE-ESI-MS) with an electrokinetically-pumped nanospray interface for the study of protein glycosylation microheterogeneity. A fast separation was developed that resolved intact glycopeptides generated from standard proteins within ~9min. Differentially terminal-galactosylated and sialylated species with the same glycosylation sites were well resolved...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29304454/impact-of-ethylene-diurea-edu-on-growth-yield-and-proteome-of-two-winter-wheat-varieties-under-high-ambient-ozone-phytotoxicity
#20
Sunil K Gupta, Marisha Sharma, Baisakhi Majumder, Vivek K Maurya, Meenakshi Lohani, Farah Deeba, Vivek Pandey
The present study evaluated the impact of high ambient O3 on morphological, physiological and biochemical traits and leaf proteome in two high-yielding varieties of wheat using ethylene diurea (EDU) as foliar spray (200 and 300 ppm). Average ambient ozone concentration was 60 ppb which was more than sufficient to cause phytotoxic effects. EDU treatment resulted in less lipid peroxidation along with increased chlorophyll content, biomass and yield. EDU alleviated the negative effects of ozone by enhancing activities of antioxidants and antioxidative enzymes...
December 27, 2017: Chemosphere
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