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https://www.readbyqxmd.com/read/28709087/novel-electrochemiluminescence-of-silver-nanoclusters-fabricated-on-triplex-dna-scaffolds-for-label-free-detection-of-biothiols
#1
Lingyan Feng, Li Wu, Feifei Xing, Lianzhe Hu, Jinsong Ren, Xiaogang Qu
Electrochemiluminescence (ECL) of metal nanoclusters and their application have been widely reported due to the good biocompatibility, fascinating electrocatalytic activity and so on. Using DNA as synthesis template opens new opportunities to modulate the physical properties of AgNCs. Triplex DNA has been reported for the site-specific, homogeneous and highly stable silver nanoclusters (AgNCs) fabrication from our recent research. Here we further explore their extraordinary ECL properties and applications in biosensor utilization...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28686662/comparison-of-a-new-multiplex-real-time-pcr-with-the-kato-katz-thick-smear-and-copro-antigen-elisa-for-the-detection-and-differentiation-of-taenia-spp-in-human-stools
#2
Dinh Ng-Nguyen, Mark A Stevenson, Pierre Dorny, Sarah Gabriël, Tinh Van Vo, Van-Anh Thi Nguyen, Trong Van Phan, Sze Fui Hii, Rebecca J Traub
BACKGROUND: Taenia solium, the cause of neurocysticercosis (NCC), has significant socioeconomic impacts on communities in developing countries. This disease, along with taeniasis is estimated to infect 2.5 to 5 million people globally. Control of T. solium NCC necessitates accurate diagnosis and treatment of T. solium taeniasis carriers. In areas where all three species of Taenia tapeworms (T. solium, Taenia saginata and Taenia asiatica) occur sympatrically, conventional microscope- and copro-antigen based diagnostic methods are unable to distinguish between these three Taenia species...
July 7, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28681829/molecular-characterisation-of-uropathogenic-escherichia-coli-isolates-at-a-tertiary-care-hospital-in-south-india
#3
Arindam Chakraborty, Prabha Adhikari, Shalini Shenoy, Vishwas Saralaya
Uropathogenic Escherichia coli (UPEC) express a multitude of virulence factors (VFs) to break the inertia of the mucosal barrier of the urinary tract. The aim of the present study was undertaken to characterised the UPEC strains and to correlate carriage of specific virulence markers with different phylogroups and also to correlate these findings with clinical outcome of patients. A total of 156 non-repeated, clinically significant UPEC isolates were studied. Virulent genes were determined by two set of multiplex polymerase chain reaction (PCR)...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28674450/triplex-dna-based-bioanalytical-platform-for-highly-sensitive-homogeneous-electrochemical-detection-of-melamine
#4
Xiaojuan Liu, Mengmeng Song, Feng Li
Melamine detection has attracted much attention since the discovery of the damage of melamine to human health. Herein, we have developed a sensitive homogeneous electroanalytical platform for melamine detection, which is relied on the formation of triplex molecular beacon integrated with exonuclease III (Exo III)-mediated signal amplification. The formation of triplex molecular beacon was triggered by the recognition and incorporation of melamine to the abasic (AP) site contained in the triplex stem. The stem of the triplex molecular beacon was designed to have a protruding double-strand DNA, which can be recognized and hydrolyzed by Exo III for releasing methylene blue (MB)-labeled mononucleotide...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28673433/low-intensity-shockwave-therapy-improves-hemodynamic-parameters-in-patients-with-vasculogenic-erectile-dysfunction-a-triplex-ultrasonography-based-sham-controlled-trial
#5
Dimitrios Kalyvianakis, Dimitrios Hatzichristou
BACKGROUND: Although several reports have documented the subjective improvement of erectile function after low-intensity extracorporeal shockwave therapy (LI-ESWT) in patients with vasculogenic erectile dysfunction (ED), objective assessment data of penile hemodynamics are lacking. AIM: To assess penile hemodynamics before and 3 months after LI-ESWT in a group of patients with documented vasculogenic ED. METHODS: This was a double-blinded, randomized, sham-controlled trial...
July 2017: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/28670571/dna-triplex-forming-purine-repeat-containing-genes-in-acinetobacter-baumannii-and-their-association-with-infection-and-adaptation
#6
Himanshu N Singh, Moganty R Rajeswari
No abstract text is available yet for this article.
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28663444/atomic-structure-of-the-human-cytomegalovirus-capsid-with-its-securing-tegument-layer-of-pp150
#7
Xuekui Yu, Jonathan Jih, Jiansen Jiang, Z Hong Zhou
Herpesviruses possess a genome-pressurized capsid. The 235-kilobase genome of human cytomegalovirus (HCMV) is by far the largest of any herpesvirus, yet it has been unclear how its capsid, which is similar in size to those of other herpesviruses, is stabilized. Here we report a HCMV atomic structure consisting of the herpesvirus-conserved capsid proteins MCP, Tri1, Tri2, and SCP and the HCMV-specific tegument protein pp150-totaling ~4000 molecules and 62 different conformers. MCPs manifest as a complex of insertions around a bacteriophage HK97 gp5-like domain, which gives rise to three classes of capsid floor-defining interactions; triplexes, composed of two "embracing" Tri2 conformers and a "third-wheeling" Tri1, fasten the capsid floor...
June 30, 2017: Science
https://www.readbyqxmd.com/read/28652006/3d-nus-a-web-server-for-automated-modeling-and-visualization-of-non-canonical-3-dimensional-nucleic-acid-structures
#8
L Ponoop Prasad Patro, Abhishek Kumar, Narendar Kolimi, Thenmalarchelvi Rathinavelan
The inherent conformational flexibility of nucleic acids facilitate the formation of a range of conformations such as duplex, triplex, quadruplex etc. that play crucial roles in biological processes. Elucidation about the influence of non-canonical base pair mismatches on DNA/RNA structures at different sequence contexts to understand mismatch repair and misregulation of alternative splicing mechanisms as well as the sequence dependent effect of RNA-DNA hybrid in relevance to antisense strategy demand their three-dimensional structural information...
June 23, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28641941/role-of-recombination-and-replication-fork-restart-in-repeat-instability
#9
REVIEW
Erica J Polleys, Nealia C M House, Catherine H Freudenreich
Eukaryotic genomes contain many repetitive DNA sequences that exhibit size instability. Some repeat elements have the added complication of being able to form secondary structures, such as hairpin loops, slipped DNA, triplex DNA or G-quadruplexes. Especially when repeat sequences are long, these DNA structures can form a significant impediment to DNA replication and repair, leading to DNA nicks, gaps, and breaks. In turn, repair or replication fork restart attempts within the repeat DNA can lead to addition or removal of repeat elements, which can sometimes lead to disease...
June 9, 2017: DNA Repair
https://www.readbyqxmd.com/read/28636339/binding-differences-of-two-homochiral-ru-bpy-2dppz-2-complexes-with-poly-u-%C3%A2-poly-a-poly-u-triplex-rna
#10
Meng-Na Peng, Zhi-Yuan Zhu, Li-Feng Tan
The first investigation of chiral ruthenium(II) complexes Δ- and Λ-[Ru(bpy)2dppz](2+) and triplex RNA poly(U)·poly(A)*poly(U) was carried out, which showed that Δ enantiomer displayed significant ability in stabilizing model triplex RNA.
June 21, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28635252/proton-fueled-reversible-dna-hybridization-chain-assembly-for-ph-sensing-and-imaging
#11
Lan Liu, Jin-Wen Liu, Zhi-Mei Huang, Han Wu, Na Li, Li-Juan Tang, Jian-Hui Jiang
Design of DNA self-assembly with reversible responsiveness to external stimuli is of great interest for diverse applications. We for the first time develop a pH-responsive, fully reversible hybridization chain reaction (HCR) assembly that allows sensitive sensing and imaging of pH in living cells. Our design relies on the triplex forming sequences that form DNA triplex with toehold regions under acidic conditions and then induce a cascade of strand displacement and DNA assembly. The HCR assembly has shown dynamic responses in physiological pH ranges with excellent reversibility and demonstrated the potential for in vitro detection and live-cell imaging of pH...
June 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28630686/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-enterobacteriaceae-in-egyptian-patients-with-community-onset-gastrointestinal-complaints-a-hospital-based-cross-sectional-study
#12
H M Abdallah, N Alnaiemi, E A Reuland, B B Wintermans, A Koek, A M Abdelwahab, A Samy, K W Abdelsalam, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among Enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt in the period between January 2013 and May 2013. METHODS: One hundred and thirteen Enterobacteriaceae isolates were recovered from 100 consecutive Egyptian patients with community-onset gastrointestinal complaints...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28627887/controlling-the-catalytic-functions-of-dnazymes-within-constitutional-dynamic-networks-of-dna-nanostructures
#13
Shan Wang, Liang Yue, Zohar Shpilt, Alessandro Cecconello, Jason S Kahn, Jean-Marie Lehn, Itamar Willner
Mimicking complex cellular dynamic chemical networks being up-regulated or down-regulated by external triggers is one of the challenges in systems chemistry. Constitutional dynamic networks (CDNs), composed of exchangeable components that respond to environmental triggers by self-adaption, provide general means to mimic biosystems. We use the structural and functional information encoded in nucleic acid nanostructures to construct effector (input)-triggered constitutional dynamic networks that reveal adaptable catalytic properties...
July 10, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28624806/targeting-the-tumor-promoting-microenvironment-in-met-amplified-nsclc-cells-with-a-novel-inhibitor-of-pro-hgf-activation
#14
Benjamin Y Owusu, Shantasia Thomas, Phanindra Venukadasula, Zhenfu Han, James W Janetka, Robert A Galemmo, Lidija Klampfer
Targeted therapeutic agents, such as inhibitors of epithelial growth factor receptor (EGFR), have transformed the management of non-small cell lung cancer (NSCLC) patients. MET-amplified NSCLC cells display resistance to EGFR-targeting agents, but are addicted to MET signaling for survival and proliferation and are sensitive to MET inhibition. However, responsive cancer cells invariably develop resistance to MET-targeted treatment.The tumor microenvironment plays a major role in resistance to anticancer therapy...
May 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28612519/glucosyltransferase-dependent-and-independent-effects-of-tcdb-on-the-proteome-of-hep-2-cells
#15
Jelena Erdmann, Johannes Junemann, Anke Schröder, Ingo Just, Ralf Gerhard, Andreas Pich
Toxin B (TcdB) of the nosocomial pathogen C. difficile has been reported to exhibit a glucosyltransferase dependent and independent effect on treated HEp-2 cells at toxin concentration above 0.3 nM. In order to investigate and further characterize both effects epithelial cells were treated with wild type TcdB and glucosyltransferase-deficient TcdBNXN and their proteomes were analyzed by LC-MS. Triplex SILAC labeling was used for quantification. Identification of 5212 and quantification of 4712 protein groups was achieved...
June 14, 2017: Proteomics
https://www.readbyqxmd.com/read/28606420/pressure-transmission-area-and-maximum-pressure-transmission-of-different-thermoplastic-resin-denture-base-materials-under-impact-load
#16
Hubban Nasution, Krid Kamonkhantikul, Mansuang Arksornnukit, Hidekazu Takahashi
PURPOSES: The purposes of the present study were to examine the pressure transmission area and maximum pressure transmission of thermoplastic resin denture base materials under an impact load, and to evaluate the modulus of elasticity and nanohardness of thermoplastic resin denture base. METHODS: Three injection-molded thermoplastic resin denture base materials [polycarbonate (Basis PC), ethylene propylene (Duraflex), and polyamide (Valplast)] and one conventional heat-polymerized acrylic resin (PMMA, SR Triplex Hot) denture base, all with a mandibular first molar acrylic resin denture tooth set in were evaluated (n=6)...
June 9, 2017: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/28605461/determining-the-folding-and-binding-free-energy-of-dna-based-nanodevices-and-nanoswitches-using-urea-titration-curves
#17
Andrea Idili, Francesco Ricci, Alexis Vallée-Bélisle
DNA nanotechnology takes advantage of the predictability of DNA interactions to build complex DNA-based functional nanoscale structures. However, when DNA functional and responsive units that are based on non-canonical DNA interactions are employed it becomes quite challenging to predict, understand and control their thermodynamics. In response to this limitation, here we demonstrate the use of isothermal urea titration experiments to estimate the free energy involved in a set of DNA-based systems ranging from unimolecular DNA-based nanoswitches to more complex DNA folds (e...
June 9, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28604066/simultaneous-identification-of-haemophilus-influenzae-and-haemophilus-haemolyticus-using-real-time-pcr
#18
Erin P Price, Tegan M Harris, Jessie Spargo, Elizabeth Nosworthy, Jemima Beissbarth, Anne B Chang, Heidi C Smith-Vaughan, Derek S Sarovich
AIM: To design a highly specific and sensitive multiplex real-time PCR assay for the differentiation of the pathogen Haemophilus influenzae from its nonpathogenic near-neighbor Haemophilus haemolyticus. MATERIALS & METHODS: A comparison of 380 Haemophilus spp. genomes was used to identify loci specific for each species. Novel PCR assays targeting H. haemolyticus (hypD) and H. influenzae (siaT) were designed. RESULTS & DISCUSSION: PCR screening across 143 isolates demonstrated 100% specificity for hypD and siaT...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28602196/sequence-specific-recognition-of-hiv-1-dsdna-in-the-large-amount-of-normal-dsdna-based-upon-nicking-enzyme-signal-amplification-and-triplex-dna
#19
Houya Zhu, Manjun Zhang, Li Zou, Ruimin Li, Liansheng Ling
A sensitive fluorescent strategy for sequence specific recognition of HIV dsDNA was established based upon Nicking Enzyme Signal Amplification (NESA) and triplex formation. dsDNA sequence from the site 7960 to site 7991 of the HIV1 dsDNA gene was designed as target dsDNA, which was composed of two complementary strands Oligonucleotide 1 with the sequence of 3'-CTT CCT TAT CTT CTT CTT CCA CCT CTC TCT CT-5' (Oligo-1) and Oligonucleotide 2 with the sequence of 5'-GAA GGA ATA GAA GAA GAA GGT GGA GAG AGA GA-3' (Oligo-2)...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28590848/exploring-the-qsar-s-predictive-truthfulness-of-the-novel-n-tuple-discrete-derivative-indices-on-benchmark-datasets
#20
O Martínez-Santiago, Y Marrero-Ponce, R Vivas-Reyes, O M Rivera-Borroto, E Hurtado, M A Treto-Suarez, Y Ramos, F Vergara-Murillo, M E Orozco-Ugarriza, Y Martínez-López
Graph derivative indices (GDIs) have recently been defined over N-atoms (N = 2, 3 and 4) simultaneously, which are based on the concept of derivatives in discrete mathematics (finite difference), metaphorical to the derivative concept in classical mathematical analysis. These molecular descriptors (MDs) codify topo-chemical and topo-structural information based on the concept of the derivative of a molecular graph with respect to a given event (S) over duplex, triplex and quadruplex relations of atoms (vertices)...
May 2017: SAR and QSAR in Environmental Research
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