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https://www.readbyqxmd.com/read/28098884/renin-angiotensin-system-activation-accelerates-atherosclerosis-in-experimental-renal-failure-by-promoting-endoplasmic-reticulum-stress-related-inflammation
#1
Jia Yang, Xi Zhang, Xinyi Yu, Weixue Tang, Hua Gan
In this study, we investigated the association between the renin-angiotensin system (RAS), endoplasmic reticulum (ER) stress and atherosclerosis (AS) in uremic apolipoprotein E knockout (apoE-/-) mice. Mild uremia was induced by a 5/6 nephrectomy (5/6 Nx) in 10-week-old apoE-/- mice. Four weeks after nephrectomy, the mice received losartan or no treatment for 16 weeks. Sham-operated mice served as the controls. We found that uremia accelerated AS at the aortic root. The activation of ER stress and the significant upregulation of pro-inflammatory cytokines and chemokines were observed in the uremic mice...
January 12, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28089882/a-highly-efficient-modified-human-serum-albumin-signal-peptide-to-secrete-proteins-in-cells-derived-from-different-mammalian-species
#2
Carolina Attallah, Marina Etcheverrigaray, Ricardo Kratje, Marcos Oggero
Signal peptides (SPs) are key elements in the production of recombinant proteins; however, little information is available concerning different SP in mammalian cells other than CHO. In order to study the efficiency of different SPs to direct the traffic along the secretory pathway of the green fluorescence protein (GFP) and a scFv-Fc fusion protein; CHO-K1, HEK293 and NS0 cell lines were transfected in a transient and stable way. SP of human azurocidin (AZ), modified human albumin (mSA), modified Cricetulus griseus Ig kappa chain V III region MOPC 63 like (mIgκ C) and modified human Ig kappa chain V III region VG (mIgκ H) were evaluated...
January 10, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28088515/the-membrane-tethered-nac-transcription-factor-atntl7-contributes-to-er-stress-resistance-in-arabidopsis
#3
Yong Hun Chi, Sarah Mae Boyles Melencion, Cresilda Vergara Alinapon, Min Ji Kim, Eun Seon Lee, Seol Ki Paeng, Joung Hun Park, Ganesh M Nawkar, Young Jun Jung, Ho Byeong Chae, Chang Ho Kang, Sang Yeol Lee
We screened for endoplasmic reticulum (ER) stress-resistant mutants among 25 mutants of the Arabidopsis NTL (NAC with Transmembrane motif 1-Like) family. We identified a novel mutant, SALK_044777, showing strong resistance to ER stress. RT-PCR and genomic DNA sequence analyses identified the mutant as atntl7, which harbors a T-DNA insertion in the fourth exon of AtNTL7. Two other atntl7-mutant alleles, in which T-DNA was inserted in the second exon and third intron of AtNTL7, respectively, showed ER-stress sensitive phenotypes, suggesting that SALK_044777 is a gain-of-function mutant...
January 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28062198/plasma-from-pre-pubertal-obese-children-impairs-insulin-stimulated-nitric-oxide-no-bioavailability-in-endothelial-cells-role-of-er-stress
#4
Natalia Di Pietro, M Loredana Marcovecchio, Sara Di Silvestre, Tommaso de Giorgis, Vincenzo Giuseppe Pio Cordone, Paola Lanuti, Francesco Chiarelli, Giuseppina Bologna, Angelika Mohn, Assunta Pandolfi
Childhood obesity is commonly associated with early signs of endothelial dysfunction, characterized by impairment of insulin signaling and vascular Nitric Oxide (NO) availability. However, the underlying mechanisms remain to be established. Hence, we tested the hypothesis that endothelial insulin-stimulated NO production and availability was impaired and related to Endoplasmic Reticulum (ER) in human umbilical vein endothelial cells (HUVECs) cultured with plasma obtained from pre-pubertal obese (OB) children...
January 3, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28057769/extra-nuclear-effects-of-estrogen-on-cortical-bone-in-males-require-er%C3%AE-af-1
#5
Helen H Farman, Jianyao Wu, Karin L Gustafsson, Sara H Windahl, Sung Hoon Kim, John A Katzenellenbogen, Claes Ohlsson, Marie K Lagerquist
Estradiol (E2) signaling via estrogen receptor alpha (ERα) is important for the male skeleton as demonstrated by ERα inactivation in both mice and man. ERα mediates estrogenic effects by translocating to the nucleus and there affecting gene transcription, but can also mediate effects via extra-nuclear actions by e.g., triggering cytoplasmic signaling cascades. ERα contains various domains and the role of activation function 1 (ERαAF-1) is known to be tissue-specific. The aim of this study was to determine the importance of extra-nuclear estrogen effects for the skeleton in males and to determine the role of ERαAF-1 for mediating these effects...
January 5, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/28055972/integrative-genomic-and-transcriptomic-analysis-for-pinpointing-recurrent-alterations-of-plant-homeodomain-genes-and-their-clinical-significance-in-breast-cancer
#6
Huimei Yu, Yuanyuan Jiang, Lanxin Liu, Wenqi Shan, Xiaofang Chu, Zhe Yang, Zeng-Quan Yang
A wide range of the epigenetic effectors that regulate chromatin modification, gene expression, genomic stability, and DNA repair contain structurally conserved domains called plant homeodomain (PHD) fingers. Alternations of several PHD finger-containing proteins (PHFs) due to genomic amplification, mutations, deletions, and translocations have been linked directly to various types of cancer. However, little is known about the genomic landscape and the clinical significance of PHFs in breast cancer. Hence, we performed a large-scale genomic and transcriptomic analysis of 98 PHF genes in breast cancer using TCGA and METABRIC datasets and correlated the recurrent alterations with clinicopathological features and survival of patients...
December 31, 2016: Oncotarget
https://www.readbyqxmd.com/read/28050634/damage-resistance-protein-dap-contributes-to-azole-resistance-in-a-sterol-regulatory-element-binding-protein-srba-dependent-way
#7
Jinxing Song, Pengfei Zhai, Ling Lu
The targeting of stress-response regulators has emerged as a powerful strategy to enhance azole drug efficacy and to abrogate azole drug resistance. Previously, we reported that a damage resistance protein (Dap) family, composed of DapA, DapB, and DapC, could respond to azole stress stimuli in Aspergillus fumigatus, although the exact response mechanisms remain unknown. In this study, RNA-seq analysis found that a total of 180 genes are induced by azole in a dapA-dependent manner. These genes are involved in oxidation-reduction, metabolic processes, and transmembrane transport...
January 3, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28041930/a-single-legionella-effector-catalyzes-a-multistep-ubiquitination-pathway-to-rearrange-tubular-endoplasmic-reticulum-for-replication
#8
Kristin M Kotewicz, Vinay Ramabhadran, Nicole Sjoblom, Joseph P Vogel, Eva Haenssler, Mengyun Zhang, Jessica Behringer, Rebecca A Scheck, Ralph R Isberg
Intracellular pathogens manipulate host organelles to support replication within cells. For Legionella pneumophila, the bacterium translocates proteins that establish an endoplasmic reticulum (ER)-associated replication compartment. We show here that the bacterial Sde proteins target host reticulon 4 (Rtn4) to control tubular ER dynamics, resulting in tubule rearrangements as well as alterations in Rtn4 associated with the replication compartment. These rearrangements are triggered via Sde-promoted ubiquitin transfer to Rtn4, occurring almost immediately after bacterial uptake...
December 22, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/28035820/genetically-encodable-bacterial-flavin-transferase-for-fluorogenic-protein-modification-in-mammalian-cells
#9
Myeong-Gyun Kang, Jumi Park, Gianfranco Balboni, Mi Hee Lim, Changwook Lee, Hyun-Woo Rhee
A bacterial flavin transferase (ApbE) was recently employed for flavin mononucleotide (FMN) modification on the Na+-translocating NADH:quinone oxidoreductase C (NqrC) protein in the pathogenic gram-negative bacterium Vibrio cholerae. We employed this unique post-translational modification in mammalian cells and found that the FMN transfer reaction robustly occurred when NqrC and ApbE were genetically targeted in the cytosol of live mammalian cells. Moreover, NqrC expression in the endoplasmic reticulum (NqrC-ER) induced the retro-translocation of NqrC to the cytosol, leading to the proteasome-mediated ER-associated degradation of NqrC, which is considered to be an innate immunological response toward the bacterial protein...
December 30, 2016: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28032645/arabidopsis-b-cell-lymphoma2-bcl-2-associated-athanogene-7-bag7-mediated-heat-tolerance-requires-translocation-sumoylation-and-binding-to-wrky29
#10
Yurong Li, Brett Williams, Martin Dickman
To cope with stress and increased accumulation of misfolded proteins, plants and animals use a survival pathway known as the unfolded protein response (UPR) that signals between the endoplasmic reticulum (ER) and the nucleus to maintain cell homeostasis via proper folding of proteins. B-cell lymphoma2 (Bcl-2)-associated athanogene (BAG) proteins are an evolutionarily conserved family of co-chaperones that are linked to disease states in mammals and responses to environmental stimuli (biotic and abiotic) in plants...
December 29, 2016: New Phytologist
https://www.readbyqxmd.com/read/28024939/rage-deficiency-alleviates-aortic-valve-calcification-in-apoe-mice-via-the-inhibition-of-endoplasmic-reticulum-stress
#11
Bo Wang, Zhejun Cai, Baoqing Liu, Zongtao Liu, Xianming Zhou, Nianguo Dong, Fei Li
Receptor for advanced glycation end products (RAGE) and endoplasmic reticulum (ER) stress have been shown to be involved in calcific aortic valve disease (CAVD). However, the association between RAGE and ER stress remains unknown in the pathogenesis of CAVD. The current study aims to test the hypothesis that RAGE deficiency alleviates aortic valve calcification via the inhibition of ER stress. Up-regulation of RAGE and ER stress markers in calcified human aortic valves were confirmed by immunoblotting. Aortic valve calcification was evaluated in atherosclerotic prone ApoE(-/-) mice or in mice with dual deficiencies of ApoE and RAGE (ApoE(-/-)RAGE(-/-)) fed with high cholesterol diet for 24weeks...
December 24, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28000699/absence-of-hikeshi-a-nuclear-transporter-for-heat-shock-protein-hsp70-causes-infantile-hypomyelinating-leukoencephalopathy
#12
Catalina Vasilescu, Pirjo Isohanni, Maarit Palomäki, Helena Pihko, Anu Suomalainen, Christopher J Carroll
Genetic leukoencephalopathies are a heterogeneous group of central nervous system disorders with white matter involvement. In a Finnish patient, we identified a novel homozygous disease-causing variant in HIKESHI, c.11G>C, p.(Cys4Ser), leading to hypomyelinating leukoencephalopathy with periventricular cysts and vermian atrophy. A founder Ashkenazi-Jewish disease-causing variant recently linked Hikeshi and its heat-shock protective function to leukoencephalopathy. In our patient, clinical features of lower limb spasticity, optic atrophy, nystagmus, and severe developmental delay were similar to reported patients...
December 21, 2016: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/27998951/a-proteomic-survey-indicates-sortilin-as-a-secondary-substrate-of-the-er-translocation-inhibitor-cyclotriazadisulfonamide-cada
#13
Victor Van Puyenbroeck, Elisa Claeys, Dominique Schols, Thomas W Bell, Kurt Vermeire
The small molecule CADA was shown to down-modulate the expression of human CD4 in a signal peptide-dependent way, through inhibition of its co-translational translocation across the ER membrane. Previous studies characterizing general glycoprotein levels and the expression of 14 different cell surface receptors showed selectivity of CADA for human CD4. Here, a PowerBlot western array was used as a screen to analyze the proteome of CADA-treated SUP-T1 human CD4+ T lymphocytes. This high-throughput monoclonal antibody panel-based immunoblotting assay of cellular signaling proteins revealed that only a small subset of the 444 detected proteins was differentially expressed after treatment with CADA...
December 20, 2016: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/27991509/protein-translocation-the-third-route-to-the-er
#14
Paulina Strzyz
No abstract text is available yet for this article.
December 19, 2016: Nature Reviews. Molecular Cell Biology
https://www.readbyqxmd.com/read/27991487/the-non-canonical-mitochondrial-inner-membrane-presequence-translocase-of-trypanosomatids-contains-two-essential-rhomboid-like-proteins
#15
Anke Harsman, Silke Oeljeklaus, Christoph Wenger, Jonathan L Huot, Bettina Warscheid, André Schneider
Mitochondrial protein import is essential for all eukaryotes. Here we show that the early diverging eukaryote Trypanosoma brucei has a non-canonical inner membrane (IM) protein translocation machinery. Besides TbTim17, the single member of the Tim17/22/23 family in trypanosomes, the presequence translocase contains nine subunits that co-purify in reciprocal immunoprecipitations and with a presequence-containing substrate that is trapped in the translocation channel. Two of the newly discovered subunits are rhomboid-like proteins, which are essential for growth and mitochondrial protein import...
December 19, 2016: Nature Communications
https://www.readbyqxmd.com/read/27989656/pathways-of-unconventional-protein-secretion
#16
REVIEW
Catherine Rabouille
Secretory proteins are conventionally transported through the endoplasmic reticulum to the Golgi and then to the plasma membrane where they are released into the extracellular space. However, numerous substrates also reach these destinations using unconventional pathways. Unconventional protein secretion (UPS) is complex and comprises cargos without a signal peptide or a transmembrane domain that can translocate across the plasma membrane, and cargos that reach the plasma membrane by bypassing the Golgi despite entering the endoplasmic reticulum (ER)...
December 15, 2016: Trends in Cell Biology
https://www.readbyqxmd.com/read/27983974/glycoprotein-quality-control-related-proteins-effectively-inhibit-fibrillation-of-amyloid-beta-1-42
#17
Kenta Kitauchi, Masafumi Sakono
Formation of amyloid beta (Aβ) aggregates is a risk factor for Alzheimer's disease. Accumulation of Aβ aggregates on the cell surface causes oxidative stress, and ultimately results in cell death. Consequently, inhibition of aggregate formation is predicted to be beneficial. Recently, translocation of glycoprotein quality control-related (GPQC) proteins such as chaperones and protein disulfide-isomerase (PDI) family members was reported under oxidative stress conditions. Therefore, it is possible that GPQC proteins contact Aβ peptides on the cell membrane during stress conditions...
October 28, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27957794/brd7-mediates-hyperglycaemia-induced-myocardial-apoptosis-via-endoplasmic-reticulum-stress-signalling-pathway
#18
Xiao-Meng Wang, Ying-Cui Wang, Xiang-Juan Liu, Qi Wang, Chun-Mei Zhang, Li-Ping Zhang, Hui Liu, Xin-Yu Zhang, Yang Mao, Zhi-Ming Ge
Bromodomain-containing protein 7 (BRD7) is a tumour suppressor that is known to regulate many pathological processes including cell growth, apoptosis and cell cycle. Endoplasmic reticulum (ER) stress-induced apoptosis plays a key role in diabetic cardiomyopathy (DCM). However, the molecular mechanism of hyperglycaemia-induced myocardial apoptosis is still unclear. We intended to determine the role of BRD7 in high glucose (HG)-induced apoptosis of cardiomyocytes. In vivo, we established a type 1 diabetic rat model by injecting a high-dose streptozotocin (STZ), and lentivirus-mediated short hairpin RNA (shRNA) was used to inhibit BRD7 expression...
December 13, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27942753/cyanophycinase-cphe-from-p-alcaligenes-produced-in-different-compartments-of-n-benthamiana-degrades-high-amounts-of-cyanophycin-in-plant-extracts
#19
Henrik Nausch, Inge Broer
One of the major constraints in pig and poultry farming is the supply of protein-rich forage, containing sufficient amounts of key amino acids such as arginine (Ufaz and Galili 2008). Since these are underrepresented in plant proteins, the usage of plants as feed is limited. The heterologous production of the cyanobacterial storage polymer cyanophycin granule polypeptide (CGP) in plastids increases the amount of arginine substantially (Huhns et al. 2008; Huhns et al. 2009; Nausch et al. 2016a). CGP degradation releases arginine-aspartate dipeptides...
December 9, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27936561/stem-cell-membrane-camouflaging-on-near-infrared-photoactivated-upconversion-nanoarchitectures-for-in-vivo-remote-controlled-photodynamic-therapy
#20
Changyong Gao, Zhihua Lin, Zhiguang Wu, Xiankun Lin, Qiang He
The upconversion nanoparticle (UCNP)-based photodynamic therapy (PDT) agents are promising for deep-tissue cancer treatment because they may overcome current limitations due to the shallow penetration depth of visible light. However, limited blood circulation time and poor tumor-targeting capability challenge the therapeutic efficacy of UCNP-based PDT in vivo. Here, we demonstrate intravenous injectable stem-cell-membrane-camouflaged upconversion nanoarchitectures as a biomimetic tumor PDT platform. The biomimetic PDT system is constructed by fusing mesoporous-silica-encapsulated β-NaYF4:Yb(3+),Er(3+) UCNPs with stem-cell membranes...
December 21, 2016: ACS Applied Materials & Interfaces
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