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https://www.readbyqxmd.com/read/28921786/lipid-production-and-characterization-by-mortierella-umbelopsis-isabellina-cultivated-on-lignocellulosic-sugars
#1
Chryssavgi Gardeli, Maria Athenaki, Evangelos Xenopoulos, Athanasios Mallouchos, Apostolis A Koutinas, George Aggelis, Seraphim Papanikolaou
AIMS: To study and characterize the lipids produced by Mortierella (Umbelopsis) isabellina, during its growth on mixtures of glucose and xylose. METHODS AND RESULTS: Glucose and xylose were utilized as carbon sources, solely or in blends, under nitrogen limited conditions, in batch-flask trials (initial sugars at 80 g l(-1) ). Significant lipid production (maximum lipid 17.8 g l(-1) ; lipid in DCW 61.0% w/w; lipid on glucose consumed 0.23 g g(-1) ) occurred on glucose employed solely, while glucose concentration in the growth medium was conversely correlated with lipid accumulation...
September 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28920460/in-situ-biobutanol-recovery-from-clostridial-fermentations-a-critical-review
#2
Pablo Jiménez-Bonilla, Yi Wang
Butanol is a precursor of many industrial chemicals, and a fuel that is more energetic, safer and easier to handle than ethanol. Fermentative biobutanol can be produced using renewable carbon sources such as agro-industrial residues and lignocellulosic biomass. Solventogenic clostridia are known as the most preeminent biobutanol producers. However, until now, solvent production through the fermentative routes is still not economically competitive compared to the petrochemical approaches, because the butanol is toxic to their own producer bacteria, and thus, the production capability is limited by the butanol tolerance of producing cells...
September 18, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28920414/metagenomic-analysis-of-bacterial-community-structure-and-diversity-of-lignocellulolytic-bacteria-in-vietnamese-native-goat-rumen
#3
Huyen Thi Do, Khoa Trong Dao, Viet Khanh Hoang Nguyen, Giang Ngoc Le, Phuong Thi Mai Nguyen, Lam Tung Le, Nguyet Thu Phung, Nico van Straalen, Dick Roelofs, Hai Nam Truong
Objective: In a previous study, analysis of Illumina sequenced metagenomic DNA data of bacteria in Vietnamese goats' rumen showed a high diversity of putative lignocellulolytic genes. In this study, taxonomy speculation of microbial community and lignocellulolytic bacteria population in the rumen was conducted to elucidate a role of bacterial structure for effective degradation of plant materials. Methods: The metagenomic data had been subjected into Basic Local Alignment Search Tool (BLASTX) algorithm and the National Center for Biotechnology Information (NCBI) non-redundant (NR) sequence database...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28919928/the-yeast-geotrichum-candidum-encodes-functional-lytic-polysaccharide-monooxygenases
#4
Simon Ladevèze, Mireille Haon, Ana Villares, Bernard Cathala, Sacha Grisel, Isabelle Herpoël-Gimbert, Bernard Henrissat, Jean-Guy Berrin
BACKGROUND: Lytic polysaccharide monooxygenases (LPMOs) are a class of powerful oxidative enzymes that have revolutionized our understanding of lignocellulose degradation. Fungal LPMOs of the AA9 family target cellulose and hemicelluloses. AA9 LPMO-coding genes have been identified across a wide range of fungal saprotrophs (Ascomycotina, Basidiomycotina, etc.), but so far they have not been found in more basal lineages. Recent genome analysis of the yeast Geotrichum candidum (Saccharomycotina) revealed the presence of several LPMO genes, which belong to the AA9 family...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28919927/characterization-of-a-heat-tolerant-chlorella-sp-gd-mutant-with-enhanced-photosynthetic-co2-fixation-efficiency-and-its-implication-as-lactic-acid-fermentation-feedstock
#5
Tse-Min Lee, Yu-Fei Tseng, Chieh-Lun Cheng, Yi-Chuan Chen, Chih-Sheng Lin, Hsiang-Yen Su, Te-Jin Chow, Chun-Yen Chen, Jo-Shu Chang
BACKGROUND: Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass. RESULTS: A Chlorella sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine to overcome outdoor high-temperature inhibition and it was used as a feedstock for fermentative lactic acid production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28919926/sustaining-fermentation-in-high-gravity-ethanol-production-by-feeding-yeast-to-a-temperature-profiled-multifeed-simultaneous-saccharification-and-co-fermentation-of-wheat-straw
#6
Johan O Westman, Ruifei Wang, Vera Novy, Carl Johan Franzén
BACKGROUND: Considerable progress is being made in ethanol production from lignocellulosic feedstocks by fermentation, but negative effects of inhibitors on fermenting microorganisms are still challenging. Feeding preadapted cells has shown positive effects by sustaining fermentation in high-gravity simultaneous saccharification and co-fermentation (SSCF). Loss of cell viability has been reported in several SSCF studies on different substrates and seems to be the main reason for the declining ethanol production toward the end of the process...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28917917/characterization-of-structural-cell-wall-polysaccharides-in-cattail-typha-latifolia-evaluation-as-potential-biofuel-feedstock
#7
Diego Rebaque, Romina Martínez-Rubio, Silvia Fornalé, Penélope García-Angulo, Ana Alonso-Simón, Jesús M Álvarez, David Caparros-Ruiz, José L Acebes, Antonio Encina
Second generation bioethanol produced from lignocellulosic biomass is attracting attention as an alternative energy source. In this study, a detailed knowledge of the composition and structure of common cattail (Typha latifolia L.) cell wall polysaccharides, obtained from stem or leaves, has been conducted using a wide set of techniques to evaluate this species as a potential bioethanol feedstock. Our results showed that common cattail cellulose content was high for plants in the order Poales and was accompanied by a small amount of cross-linked polysaccharides...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917866/rapidly-growing-vegetables-as-new-sources-for-lignocellulose-nanofibre-isolation-physicochemical-thermal-and-rheological-characterisation
#8
Eduardo Espinosa, Rafael Sánchez, Zoilo González, Juan Domínguez-Robles, Begoña Ferrari, Alejandro Rodríguez
Rapidly growing vegetables could be an abundant and cheap sources of lignocellulose biomass for lignocellulose nanofibre (LCNF) production. The aim of this work is to study the feasibility of using Chamaecytisus proliferus and Leucaena leucocephala for LCNF isolation by mechanical, enzymatic and TEMPO-mediated oxidation pre-treatments. Characterisation of the nanofibres shows that there are significant differences in the production of LCNF depending on the raw material and pre-treatment used. XRD and FTIR analysis show that homogenisation has a strong negative effect on the crystallinity index; however, the higher lignin content of tagasaste (10%) protects the fibre, causing a smaller decrease in crystallinity...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917109/a-%C3%AE-xylosidase-hyper-production-penicillium-oxalicum-mutant-enhanced-ethanol-production-from-alkali-pretreated-corn-stover
#9
Yanxin Ye, Xuezhi Li, Yuan Cao, Jian Du, Shicheng Chen, Jian Zhao
β-Xylosidase activity is deficient in most cellulase enzymes secreted by filamentous fungi, which limits effective enzymatic hydrolysis of hemicellulose in lignocellulose materials and resulted in accumulation of xylo-oligosaccharides that inhibit the cellulase and xylanase activitives. An endogenous β-xylosidase gene, xyl3A, was overexpressed using two types of promoters in cellulolytic P. oxalicum RE-10. The mutants RXyl, RGXyl-1 and RGXyl-2 displayed higher β-xylosidase production than native strain RE-10 besides higher cellulase and xylanase activities, especially RGXyl-1, showing the highest β-xylosidase activity of 15...
August 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28916814/expression-of-brown-midrib-in-a-spontaneous-sorghum-mutant-is-linked-to-a-5-utr-deletion-in-lignin-biosynthesis-gene-sbcad2
#10
Huang Li, Yinghua Huang
Brown midrib (bmr) mutants in sorghum (Sorghum bicolor (L.) Moench) and several other C4 grasses are associated with reduced lignin concentration, altered lignin composition and improved cell wall digestibility, which are desirable properties in biomass development for the emerging lignocellulosic biofuel industry. Studying bmr mutants has considerably expanded our understanding of the molecular basis underlying lignin biosynthesis and perturbation in grasses. In this study, we performed quantitative trait locus (QTL) analysis, identified and cloned a novel cinnamyl alcohol dehydrogenase allele (SbCAD2) that has an 8-bp deletion in its 5'-untranslated region (UTR), conferring the spontaneous brown midrib trait and lignin reduction in the sorghum germplasm line PI 595743...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28913738/comparison-of-the-efficiency-of-bacterial-and-fungal-laccases-in-delignification-and-detoxification-of-steam-pretreated-lignocellulosic-biomass-for-bioethanol-production
#11
María De La Torre, Raquel Martín-Sampedro, Úrsula Fillat, María E Eugenio, Alba Blánquez, Manuel Hernández, María E Arias, David Ibarra
This study evaluates the potential of a bacterial laccase from Streptomyces ipomoeae (SilA) for delignification and detoxification of steam-exploded wheat straw, in comparison with a commercial fungal laccase from Trametes villosa. When alkali extraction followed by SilA laccase treatment was applied to the water insoluble solids fraction, a slight reduction in lignin content was detected, and after a saccharification step, an increase in both glucose and xylose production (16 and 6%, respectively) was observed...
September 14, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28912833/perspectives-for-biocatalytic-lignin-utilization-cleaving-4-o-5-and-c%C3%AE-c%C3%AE-bonds-in-dimeric-lignin-model-compounds-catalyzed-by-a-promiscuous-activity-of-tyrosinase
#12
Kyoungseon Min, Taewoo Yum, Jiye Kim, Han Min Woo, Yunje Kim, Byoung-In Sang, Young Je Yoo, Yong Hwan Kim, Youngsoon Um
BACKGROUND: In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date. RESULTS: Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28912429/production-and-characteristics-of-a-novel-xylose-and-alkali-tolerant-gh-43-%C3%AE-xylosidase-from-penicillium-oxalicum-for-promoting-hemicellulose-degradation
#13
Yanxin Ye, Xuezhi Li, Jian Zhao
β-xylosidase is a pivotal enzyme for complete degradation of xylan in hemicelluloses of lignocelluloses, and the xylose- and alkali-tolerant β-xylosidase with high catalytic activity is very attractive for promoting enzymatic hydrolysis of alkaline-pretreated lignocellulose. In this study, a novel intracellular glycoside hydrolase family 43 β-xylosidase gene (xyl43) from Penicillium oxalicum 114-2 was successfully high-level overexpressed in Pichia pastoris, and the secreted enzyme was characterized. The β-xylosidase Xyl43 exhibited great pH stability and high catalytic activity in the range of pH 6...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28912079/solid-anaerobic-digestion-state-of-art-scientific-and-technological-hurdles
#14
REVIEW
Laura André, André Pauss, Thierry Ribeiro
In this paper, a state-of-art about solid anaerobic digestion (AD), focused on recent progress and trends of research is proposed. Solid anaerobic digestion should be the most appropriate process for degradation of by-products with high total solid (TS) content, especially lignocellulosic materials like agricultural waste (straw, manure), household waste and food waste. Solid AD is already widely used in waste water treatment plant for treating plant for sewage sludge but could be more developed for lignocellulosic materials with high TS content...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28910646/minimizing-mixing-intensity-to-improve-the-performance-of-rice-straw-anaerobic-digestion-via-enhanced-development-of-microbe-substrate-aggregates
#15
Moonkyung Kim, Byung-Chul Kim, Yongju Choi, Kyoungphile Nam
The aim of this work was to study the effect of the differential development of microbe-substrate aggregates at different mixing intensities on the performance of anaerobic digestion of rice straw. Batch and semi-continuous reactors were operated for up to 50 and 300days, respectively, under different mixing intensities. In both batch and semi-continuous reactors, minimal mixing conditions exhibited maximum methane production and lignocellulose biodegradability, which both had strong correlations with the development of microbe-substrate aggregates...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28905511/talen-mediated-targeted-mutagenesis-of-more-than-100-comt-copies-alleles-in-highly-polyploid-sugarcane-improves-saccharification-efficiency-without-compromising-biomass-yield
#16
Baskaran Kannan, Je Hyeong Jung, Geoffrey W Moxley, Sun-Mi Lee, Fredy Altpeter
Sugarcane is the world's most efficient feedstock for commercial production of bioethanol due to its superior biomass production and accumulation of sucrose in stems. Integrating first and second generation ethanol conversion processes will enhance the biofuel yield per unit area by utilizing both sucrose as well as cell wall bound sugars for fermentation. RNAi suppression of the lignin biosynthetic gene caffeic acid O-methyltransferase (COMT) has been demonstrated to improve bioethanol production from lignocellulosic biomass...
September 14, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28904563/bacillus-coagulans-ma-13-a-promising-thermophilic-and-cellulolytic-strain-for-the-production-of-lactic-acid-from-lignocellulosic-hydrolysate
#17
Martina Aulitto, Salvatore Fusco, Simonetta Bartolucci, Carl Johan Franzén, Patrizia Contursi
BACKGROUND: The transition from a petroleum-based economy towards more sustainable bioprocesses for the production of fuels and chemicals (circular economy) is necessary to alleviate the impact of anthropic activities on the global ecosystem. Lignocellulosic biomass-derived sugars are suitable alternative feedstocks that can be fermented or biochemically converted to value-added products. An example is lactic acid, which is an essential chemical for the production of polylactic acid, a biodegradable bioplastic...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28904370/in-planta-expression-of-hyperthermophilic-enzymes-as-a-strategy-for-accelerated-lignocellulosic-digestion
#18
Bilal Ahmad Mir, Alexander A Myburg, Eshchar Mizrachi, Don A Cowan
Conversion of lignocellulosic biomass to biofuels and biomaterials suffers from high production costs associated with biomass pretreatment and enzymatic hydrolysis. In-planta expression of lignocellulose-digesting enzymes is a promising approach to reduce these cost elements. However, this approach faces a number of challenges, including auto-hydrolysis of developing cell walls, plant growth and yield penalties, low expression levels and the limited stability of expressed enzymes at the high temperatures generally used for biomass processing to release fermentable sugars...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28901274/revalorizing-lignocellulose-for-the-production-of-natural-pharmaceuticals-and-other-high-value-bioproducts
#19
Heng-Phon Too, Congqiang Zhang
Lignocellulose is the most plentiful, renewable natural resource on earth and has been successfully used for the production of biofuels. A significant challenge is to develop cost-effective, environmentally friendly and efficient processes for the conversion of lignocellulose material into suitable substrates for biotransformation. A number of approaches have been explored to convert lignocellulose into sugars, e.g. combining chemical pretreatment and enzymatic hydrolysis. In nature, there are organisms that can biotransform the complex lignocellulose efficiently, such as wood-degrading fungi (brown rot and white rot fungi), bacteria (e...
September 11, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28900905/biodegradation-of-ramie-stalk-by-flammulina-velutipes-mushroom-production-and-substrate-utilization
#20
Chunliang Xie, Wenbing Gong, Li Yan, Zuohua Zhu, Zhenxiu Hu, Yuande Peng
In the textile industry, ramie stalk is byproducts with a low economic value. The potential use of this leftover as a substrate ingredient for Flammulina velutipes (F. velutipe) cultivation was evaluated. The degradation and utilization of ramie stalk by F. velutipes was evaluated through mushroom production, lignocelluloses degradation and lignocellulolytic enzymes activity. The best substrate mixture for F. velutipes cultivation comprised 50% ramie stalk, 20% cottonseed hulls, 25% wheat bran, 4% cornstarch and 2% CaCO3...
September 12, 2017: AMB Express
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