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https://www.readbyqxmd.com/read/27914260/the-extent-of-fermentative-transformation-of-phenolic-compounds-in-the-bioanode-controls-exoelectrogenic-activity-in-a-microbial-electrolysis-cell
#1
Xiaofei Zeng, Maya A Collins, Abhijeet P Borole, Spyros G Pavlostathis
Phenolic compounds in hydrolysate/pyrolysate and wastewater streams produced during the pretreatment of lignocellulosic biomass for biofuel production present a significant challenge in downstream processes. Bioelectrochemical systems are increasingly recognized as an alternative technology to handle biomass-derived streams and to promote water reuse in biofuel production. Thus, a thorough understanding of the fate of phenolic compounds in bioanodes is urgently needed. The present study investigated the biotransformation of three structurally similar phenolic compounds (syringic acid, SA; vanillic acid, VA; 4-hydroxybenzoic acid, HBA), and their individual contribution to exoelectrogenesis in a microbial electrolysis cell (MEC) bioanode...
November 27, 2016: Water Research
https://www.readbyqxmd.com/read/27913172/efficient-catalytic-system-for-the-direct-transformation-of-lignocellulosic-biomass-to-furfural-and-5-hydroxymethylfurfural
#2
Luxin Zhang, Guoyun Xi, Jiaxin Zhang, Hongbing Yu, Xiaochang Wang
A feasible approach was developed for the co-production of 5-hydroxymethylfurfural (5-HMF) and furfural from corncob via a new porous polytriphenylamine-SO3H (SPTPA) solid acid catalyst in lactone solvents. XRD, SEM, XPS, N2 adsorption-desorption, elemental analysis, TG-DTA, acid-base titration and FTIR spectroscopy techniques were used to characterize the catalyst. This study demonstrates and optimizes the catalytic performance of SPTPA and solvent selection. SPTPA was found to exhibit superior catalytic ability in γ-valerolactone (GVL)...
November 25, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27913169/liquid-nitrogen-pretreatment-of-eucalyptus-sawdust-and-rice-hull-for-enhanced-enzymatic-saccharification
#3
Rafael Castoldi, Vanesa G Correa, Gutierrez Rodrigues de Morais, Cristina G M de Souza, Adelar Bracht, Rosely A Peralta, Regina F Peralta-Muniz Moreira, Rosane M Peralta
In this work, liquid nitrogen was used for the first time in the pretreatment of plant biomasses for purposes of enzymatic saccharification. After treatment (cryocrushing), the initial rates of the enzymatic hydrolysis of eucalyptus sawdust and rice hull were increased more than ten-fold. Cryocrushing did not modify significantly the contents of cellulose, hemicellulose and lignin in both eucalyptus sawdust and rice hulls. However, substantial disorganization of the lignocellulosic materials in consequence of the pretreatment could be observed by electron microscopy...
November 25, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27913168/fermentative-production-of-high-titer-citric-acid-from-corn-stover-feedstock-after-dry-dilute-acid-pretreatment-and-biodetoxification
#4
Ping-Ping Zhou, Jiao Meng, Jie Bao
The aim of this work is to study the citric acid fermentation by a robust strain Aspergillus niger SIIM M288 using corn stover feedstock after dry dilute sulfuric acid pretreatment and biodetoxification. Citric acid at 100.04g/L with the yield of 94.11% was obtained, which are comparable to the starch or sucrose based citric acid fermentation. No free wastewater was generated in the overall process from the pretreatment to citric acid fermentation. Abundant divalent metal ions as well as high titer of potassium, phosphate, and nitrogen were found in corn stover hydrolysate...
November 15, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27910761/investigation-of-requisites-for-the-optimal-mycelial-growth-of-the-lingzhi-or-reishi-medicinal-mushroom-ganoderma-lucidum-agaricomycetes-on-oil-palm-biomass-in-malaysia
#5
Surya Sudheer, Asgar Ali, Sivakumar Manickam
Rigorous research has been carried out regarding the cultivation of Ganoderma lucidum using different agricultural residues. Nevertheless, large-scale cultivation and the separation of active compounds of G. lucidum are still challenges for local farmers. The objective of this study was to evaluate the use of oil palm waste fibers such as empty fruit bunch fibers and mesocarp fibers as effective substrates for the growth of G. lucidum mycelia to study the possibility of solid-state cultivation and to determine the optimum conditions necessary for the growth of mycelia of this mushroom on these waste fibers...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27908585/innovative-pretreatment-strategies-for-biogas-production
#6
REVIEW
Regina J Patinvoh, Osagie A Osadolor, Konstantinos Chandolias, Ilona Sárvári Horváth, Mohammad J Taherzadeh
Biogas or biomethane is traditionally produced via anaerobic digestion, or recently by thermochemical or a combination of thermochemical and biological processes via syngas (CO and H2) fermentation. However, many of the feedstocks have recalcitrant structure and are difficult to digest (e.g., lignocelluloses or keratins), or they have toxic compounds (such as fruit flavors or high ammonia content), or not digestible at all (e.g., plastics). To overcome these challenges, innovative strategies for enhanced and economically favorable biogas production were proposed in this review...
November 22, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27905534/pore-scale-dynamics-of-enzyme-adsorption-swelling-and-reactive-dissolution-determine-sugar-yield-in-hemicellulose-hydrolysis-for-biofuel-production
#7
Sajal Kanti Dutta, Saikat Chakraborty
Hemicelluloses are the earth's second most abundant structural polymers, found in lignocellulosic biomass. Efficient enzymatic depolymerization of xylans by cleaving their β-(1 → 4)-glycosidic bonds to produce soluble sugars is instrumental to the cost-effective production of liquid biofuels. Here we show that the multi-scale two-phase process of enzymatic hydrolysis of amorphous hemicelluloses is dominated by its smallest scale-the pores. In the crucial first five hours, two to fourfold swelling of the xylan particles allow the enzymes to enter the pores and undergo rapid non-equilibrium adsorption on the pore surface before they hydrolyze the solid polymers, albeit non-competitively inhibited by the products xylose and xylobiose...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27905050/transcriptional-analysis-of-genes-encoding-%C3%AE-glucosidase-of-schizophyllum-commune-kuc9397-under-optimal-conditions
#8
Young Min Lee, Hanbyul Lee, Young Mok Heo, Hwanhwi Lee, Joo-Hyun Hong, Jae-Jin Kim
The present study was conducted to determine the gene responsible for beta-glucosidase (BGL) production and to generate a full-length complementary DNA (cDNA) of one of the putative BGL genes, which showed a significant expression level when Schizophyllum commune KUC9397 was grown in optimized medium. The relative expression levels of seven genes encoding BGL of S. commune KUC9397 were determined with real-time quantitative reverse transcription PCR in cellulose-containing optimized medium (OM) compared to glucose-containing basal medium (BM)...
November 30, 2016: Folia Microbiologica
https://www.readbyqxmd.com/read/27900692/liquid-phase-multiplex-high-throughput-screening-of-metagenomic-libraries-using-p-nitrophenyl-linked-substrates-for-accessory-lignocellulosic-enzymes
#9
Mariette Smart, Robert J Huddy, Don A Cowan, Marla Trindade
To access the genetic potential contained in large metagenomic libraries, suitable high-throughput functional screening methods are required. Here we describe a high-throughput screening approach which enables the rapid identification of metagenomic library clones expressing functional accessory lignocellulosic enzymes. The high-throughput nature of this method hinges on the multiplexing of both the E. coli metagenomic library clones and the colorimetric p-nitrophenyl linked substrates which allows for the simultaneous screening for β-glucosidases, β-xylosidases, and α-L-arabinofuranosidases...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27899337/furfural-and-glucose-can-enhance-conversion-of-xylose-to-xylitol-by-candida-magnoliae-tistr-5663
#10
Siwaporn Wannawilai, Wen-Chien Lee, Yusuf Chisti, Sarote Sirisansaneeyakul
Xylitol production from xylose by the yeast Candida magnoliae TISTR 5663 was enhanced by supplementing the fermentation medium with furfural (300mg/L) and glucose (3g/L with an initial mass ratio of glucose to xylose of 1:10) together under oxygen limiting conditions. In the presence of furfural and glucose, the final concentration of xylitol was unaffected relative to control cultures but the xylitol yield on xylose increased by about 5%. Supplementation of the culture medium with glucose alone at an initial concentration of 3g/L, stimulated the volumetric and specific rates of xylose consumption and the rate of xylitol production from xylose...
November 26, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27898806/genome-wide-association-study-for-nine-plant-architecture-traits-in-sorghum
#11
Jing Zhao, Maria B Mantilla Perez, Jieyun Hu, Maria G Salas Fernandez
Sorghum [ (L) Moench], an important grain and forage crop, is receiving significant attention as a lignocellulosic feedstock because of its water-use efficiency and high biomass yield potential. Because of the advancement of genotyping and sequencing technologies, genome-wide association study (GWAS) has become a routinely used method to investigate the genetic mechanisms underlying natural phenotypic variation. In this study, we performed a GWAS for nine grain and biomass-related plant architecture traits to determine their overall genetic architecture and the specific association of allelic variants in gibberellin (GA) biosynthesis and signaling genes with these phenotypes...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27898315/single-step-single-organism-bioethanol-production-and-bioconversion-of-lignocellulose-waste-materials-by-phlebioid-fungal-species
#12
Hans Mattila, Jaana Kuuskeri, Taina Lundell
Ethanol production from non-pretreated lignocellulose was carried out in a consolidated bioprocess with wood-decay fungi of phlebioid Polyporales. Ethanol production was attempted on glucose, spruce wood sawdust and waste core board. Substantial quantities of ethanol were achieved, and isolate Phlebia radiata 0043 produced 5.9g/L of ethanol reaching the yield of 10.4% ethanol from core board lignocellulose substrate. Acidic initial culture conditions (pH 3) induced ethanol fermentation compared to the more neutral environment...
November 22, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27898314/effect-of-micro-aeration-and-inoculum-type-on-the-biodegradation-of-lignocellulosic-substrate
#13
P Tsapekos, P G Kougias, S A Vasileiou, G Lyberatos, I Angelidaki
The effect of various micro-aeration strategies on the anaerobic digestion (AD) of wheat straw was thoroughly examined using a mixture of inocula, containing compost and well digested sludge from biogas plant. The aim was to determine the most efficient oxygen load, pulse repetition and treatment duration, resulting in the highest methane production. The oxygen load had the largest impact on the biodegradability of straw, among the examined variables. More specifically, a micro-aeration intensity of 10mLO2/gVS was identified as the critical threshold above which the AD performance was more susceptible to instability...
November 22, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27898203/biorefining-of-lignocellulosic-feedstock-by-a-modified-ammonia-fiber-expansion-pretreatment-and-enzymatic-hydrolysis-for-production-of-fermentable-sugars
#14
Birgit Kamm, Sebastian Leiß, Petra Schönicke, Matthias Bierbaum
Wheat straw was pretreated and afterwards enzymatically hydrolyzed using a modified ammonia fiber expansion (AFEX) process under different reaction conditions to produce fermentable sugars. Instead of liquid ammonia, aqueous ammonia (25 % w/v) was used to test its influence on the sugar concentration and yield of the sugars. It is shown that a protein extraction after the pretreatment can distinctly improve the result obtained for the enzymatic hydrolysis. This modified AFEX process using aqueous ammonia represents a simpler and less expensive variant of the AFEX process usually described in literature...
November 29, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27895708/continuous-production-of-biohythane-from-hydrothermal-liquefied-cornstalk-biomass-via-two-stage-high-rate-anaerobic-reactors
#15
Bu-Chun Si, Jia-Ming Li, Zhang-Bing Zhu, Yuan-Hui Zhang, Jian-Wen Lu, Rui-Xia Shen, Chong Zhang, Xin-Hui Xing, Zhidan Liu
BACKGROUND: Biohythane production via two-stage fermentation is a promising direction for sustainable energy recovery from lignocellulosic biomass. However, the utilization of lignocellulosic biomass suffers from specific natural recalcitrance. Hydrothermal liquefaction (HTL) is an emerging technology for the liquefaction of biomass, but there are still several challenges for the coupling of HTL and two-stage fermentation. One particular challenge is the limited efficiency of fermentation reactors at a high solid content of the treated feedstock...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27895707/fungal-fermentation-on-anaerobic-digestate-for-lipid-based-biofuel-production
#16
Yuan Zhong, Zhiguo Liu, Christine Isaguirre, Yan Liu, Wei Liao
BACKGROUND: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27893787/selection-signatures-in-four-lignin-genes-from-switchgrass-populations-divergently-selected-for-in-vitro-dry-matter-digestibility
#17
Shiyu Chen, Shawn M Kaeppler, Kenneth P Vogel, Michael D Casler
Switchgrass is undergoing development as a dedicated cellulosic bioenergy crop. Fermentation of lignocellulosic biomass to ethanol in a bioenergy system or to volatile fatty acids in a livestock production system is strongly and negatively influenced by lignification of cell walls. This study detects specific loci that exhibit selection signatures across switchgrass breeding populations that differ in in vitro dry matter digestibility (IVDMD), ethanol yield, and lignin concentration. Allele frequency changes in candidate genes were used to detect loci under selection...
2016: PloS One
https://www.readbyqxmd.com/read/27891177/furfural-tolerance-and-detoxification-mechanism-in-candida-tropicalis
#18
Shizeng Wang, Gang Cheng, Chijioke Joshua, Zijun He, Xinxiao Sun, Ruimin Li, Lexuan Liu, Qipeng Yuan
BACKGROUND: Current biomass pretreatment by hydrothermal treatment (including acid hydrolysis, steam explosion, and high-temperature steaming) and ionic liquids generally generate inhibitors to the following fermentation process. Furfural is one of the typical inhibitors generated in hydrothermal treatment of biomass. Furfural could inhibit cell growth rate and decrease biofuel productivity of microbes. Candida tropicalis is a promising microbe for the production of biofuels and value-added chemicals using hemicellulose hydrolysate as carbon source...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27888663/effect-of-structural-carbohydrates-and-lignin-content-on-the-anaerobic-digestion-of-paper-and-paper-board-materials-by-anaerobic-granular-sludge
#19
Jorge Gonzalez-Estrella, Caitlin M Asato, Amber C Jerke, James J Stone, Patrick C Gilcrease
Anaerobic digestion (AD) of lignocellulosic materials is commonly limited by the hydrolysis step. Unlike unprocessed lignocellulosic materials, paper and paper board (PPB) are processed for their fabrication. Such modifications may affect their methane yields and methane production rates. Previous studies have investigated the correlation between lignin and biomethane yields of unprocessed lignocellulosic materials; nevertheless, there is limited knowledge regarding the relationship between the AD kinetic parameters and composition of PPB...
November 26, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27888662/toward-high-solids-loading-process-for-lignocellulosic-biofuel-production-at-a-low-cost
#20
Mingjie Jin, Cory Sarks, Bryan D Bals, Nick Posawatz, Christa Gunawan, Bruce E Dale, Venkatesh Balan
High solids loadings (>18 wt%) in enzymatic hydrolysis and fermentation are desired for lignocellulosic biofuel production at a high titer and low cost. However, sugar conversion and ethanol yield decrease with increasing solids loading. The factor(s) limiting sugar conversion at high solids loading is not clearly understood. In the present study, we investigated the effect of solids loading on simultaneous saccharification and co-fermentation (SSCF) of AFEX(TM) (ammonia fiber expansion) pretreated corn stover for ethanol production using a xylose fermenting strain Saccharomyces cerevisiae 424A(LNH-ST)...
November 26, 2016: Biotechnology and Bioengineering
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