Read by QxMD icon Read

Diagnostic tool

Alex Evangelista do Amaral, Michele Patrícia Rode, Julia Cisilotto, Telma Erotides da Silva, Josiane Fischer, Camila Matiollo, Elayne Cristina de Morais Rateke, Janaína Luz Narciso-Schiavon, Leonardo Lucca Schiavon, Tânia Beatriz Creczynski-Pasa
MicroRNAs (miRNAs) have remarkable potential as diagnostic and prognostic markers because of their roles in disease pathogenesis. miRNAs can be released into the bloodstream, where they are sufficiently stable to be detected noninvasively. Here, we prospectively evaluated serum levels of miR-21, miR-34a, miR-122, miR-181b, and miR-885-5p in patients with stable cirrhosis. Total RNA was extracted from the sera of patients with cirrhosis and healthy individuals, and the expression levels of the target miRNAs were analyzed by reverse transcription-quantitative polymerase chain reaction...
June 20, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Linoj P Samuel
Clinical microbiology is a highly challenging technical discipline, which makes it difficult to harmonize processes and protocols. In addition, the lack of scientific consensus on some diagnostic algorithms and the need to address the diverse needs of different patient populations contribute to the lack of interlaboratory consistency. Laboratories utilize a number of measures and metrics, but the lack of standardized approaches and data collection means that they cannot effectively evaluate their performance against their peers...
June 23, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Sean A Virani, Vinod Sharma, Mary McCann, Jodi Koehler, Bernice Tsang, Shelley Zieroth
AIMS: The primary aim of the TRIAGE-HF trial was to correlate cardiac implantable electronic device-generated heart failure risk status (HFRS) with signs, symptoms, and patient behaviours classically associated with worsening heart failure (HF). METHODS AND RESULTS: TRIAGE-HF enrolled 100 subjects with systolic HF implanted with a Medtronic high-performance device and followed up at three Canadian HF centres. Study follow-up was up to 8 months. The HFRS assigned each subject's overall risk of HF hospitalization in the next 30 days and also highlighted abnormal device parameters contributing to a patient's risk status at the time of remote data transmission...
June 22, 2018: ESC Heart Failure
Elena V Kostina, Alexander N Sinyakov, Vladimir A Ryabinin
A variant of the hybridization oligonucleotide microarray, utilizing the principle of many probes-one spot (MPOS-microarrays), is proposed. A case study based on Orthopoxviruses (Variola, Monkeypox, and Ectromelia viruses) demonstrates a considerable increase in the fluorescence signal (up to 100-fold) when several oligonucleotide probes are printed to one spot. Moreover, the specificity of detection also increases (almost 1000-fold), allowing the use of probes that individually lack such high specificity. The optimal probes have a Tm of 32-37 °C and length of 13-15 bases...
June 22, 2018: Analytical and Bioanalytical Chemistry
T Godel, M Pham
CLINICAL/METHODICAL ISSUE: Entrapment syndromes of peripheral nerves at the elbow are common and are often diagnostically challenging disorders. Difficulties consist in lesion localization and recognition of complex spatial lesion patterns as well as in differentiation of focal and multifocal disorders. STANDARD DIAGNOSTIC METHODS: Medical history taking, neurological examination and neurophysiological tests represent the gold standard in the diagnosis of peripheral nerve lesions at the elbow, but have known methodical limitations...
June 22, 2018: Der Radiologe
Mariela Mosheva, Virginie Pouillard, Yael Fishman, Lydia Dubourg, Dafna Sofrin-Frumer, Yaffa Serur, Abraham Weizman, Stephan Eliez, Doron Gothelf, Maude Schneider
22q11.2 deletion syndrome (22q11.2DS) is the most common known microdeletion in humans occurring in 1 out of 2000-4000 live births, with increasing numbers of individuals with the microdeletion living into adulthood. The aim of the study was to explore the education and employment trajectories of individuals with 22q11.2DS from childhood to adulthood in a large cohort composed of two significant samples. 260 individuals with 22q11.2DS, 134 male and 126 female, aged 5-59 years (mean age 21.3 ± 10.8 years) were evaluated at two sites, Geneva (GVA) and Tel Aviv (TA)...
June 22, 2018: European Child & Adolescent Psychiatry
Zachary D W Dezman, David A Gorelick, Carl A Soderstrom
BACKGROUND: Non-alcohol substance use disorders (drug use disorders [DUDs]) are common in trauma patients. OBJECTIVE: To determine the test characteristics of a 4-item drug CAGE questionnaire to detect DUDs in a cohort of adult trauma inpatients. METHODS: Observational cross-sectional cohort of 1,115 adult patients admitted directly to a level-one trauma center between September, 1994 and November, 1996. All participants underwent both a 4-item drug CAGE questionnaire and the substance use disorder section of a structured psychiatric diagnostic clinical interview (SCID) (DSM-IIIR criteria), administered by staff unaware of their clinical status...
June 13, 2018: Injury
Lorre S Atlan, Ingrid S Lan, Colin Smith, Susan S Margulies
BACKGROUND: Traumatic brain injury is a leading cause of cognitive and behavioral deficits in children in the US each year. None of the current diagnostic tools, such as quantitative cognitive and balance tests, have been validated to identify mild traumatic brain injury in infants, adults and animals. In this preliminary study, we report a novel, quantitative tool that has the potential to quickly and reliably diagnose traumatic brain injury and which can track the state of the brain during recovery across multiple ages and species...
June 1, 2018: Clinical Biomechanics
Daniele Andreini, Saima Mushtaq, Gianluca Pontone, Edoardo Conte, Jeroen Sonck, Carlos Collet, Marco Guglielmo, Andrea Baggiano, Daniela Trabattoni, Stefano Galli, Piero Montorsi, Cristina Ferrari, Franco Fabbiocchi, Stefano De Martini, Andrea Annoni, Maria Elisabetta Mancini, Alberto Formenti, Marco Magatelli, Marta Resta, Elisa Consiglio, Giuseppe Muscogiuri, Cesare Fiorentini, Antonio L Bartorelli, Mauro Pepi
BACKGROUND: Recent studies demonstrated a significant improvement in the diagnostic performance of coronary CT angiography (CCTA) for the evaluation of in-stent restenosis (ISR). However, coronary stent assessment is still challenging, especially because of beam-hardening artifacts due to metallic stent struts and high atherosclerotic burden of non-stented segments. Adenosine-stress myocardial perfusion assessed by CT (CTP) recently demonstrated to be a feasible and accurate tool for evaluating the functional significance of coronary stenoses in patients with suspected coronary artery disease (CAD)...
June 18, 2018: Journal of Cardiovascular Computed Tomography
Tarig M S Alnour
Tuberculosis is one of the global health problems, the estimated deaths due to TB was around 2 million in the year 2013. Failure in early diagnosis and providing suitable treatment leads to increase the prognosis of the disease. Smear microscopy is used in many countries as a primary diagnosis of TB especially in the district poor facility laboratories, where smear negative frequency is high. This review aimed to reflects the importance of smear negative tuberculosis as a source of infection and poor prognosis of TB treatment and prevention...
July 2018: Indian Journal of Tuberculosis
Jing Peng, Nan Pang, Ying Wang, Xiao-Le Wang, Jian Chen, Juan Xiong, Pan Peng, Can-Hui Zhu, Miriam Barakael Kessi, Fang He, Fei Yin
BACKGROUND: The purposes of this study were three-fold: (i) to determine the contribution of known genes to the causation of a broad-spectrum of pediatric drug-resistant epilepsy (DRE), (ii) to compare the diagnostic yield and cost among different next-generation sequencing (NGS) approaches, and especially (iii) to assess how NGS approaches can benefit patients by improving diagnosis and treatment efficiency. METHODS: This study enrolled 273 pediatric DRE patients with no obvious acquired etiology...
June 22, 2018: CNS Neuroscience & Therapeutics
Ragab M Fereig, Hanan H Abdelbaky, Fumiaki Ihara, Yoshifumi Nishikawa
Cryptosporidium parvum is a major cause of diarrhea among human and calves, resulting in severe health hazards and drastic economic losses, respectively. Although C. parvum infection leads to high morbidity and mortality in immunocompromised patients and bovine calves, this infection remains a neglected disease. Currently available diagnostic tests for C. parvum are primarily based on detection of oocysts, DNA, or secreted antigens in fecal specimens. Demonstration of specific antibodies with a rapid immunochromatographic test (ICT) will be advantageous not only in providing a simple, rapid, accurate, and affordable tool but also in surveillance because of the ability to recognize recent and past infections...
June 19, 2018: Acta Tropica
Alessandra Narciso Garcia, Lucíola da Cunha Menezes Costa, Fabrício Soares de Souza, Matheus Oliveira de Almeida, Amanda Costa Araujo, Mark Hancock, Leonardo Oliveira Pena Costa
Study Design Systematic review. Background An updated summary of the evidence for the reliability of Mechanical Diagnosis and Therapy (MDT) System in patients with spinal pain is needed. Objective To investigate the evidence on the intra and inter-rater reliability of MDT system in patients with spinal pain. Methods Search strategies on MEDLINE, CINAHL, EMBASE, PEDro and Scopus were conducted. We included any study design as long as reliability of the MDT method was tested in patients with spinal pain. We collected data on the reliability of MDT to identify: main and sub-syndromes, directional preference, centralization phenomenon and lateral shift...
June 22, 2018: Journal of Orthopaedic and Sports Physical Therapy
Marina Bockaj, Barnabas Fung, Michael Tsoulis, Lauren Warren Foster, Leyla Soleymani
Currently, a blood test for the diagnosis of endometriosis, a common estrogen-dependent gynecological disease, does not exist. Recent studies suggest that circulating concentrations of brain derived neurotrophic factor (BDNF) have potential for the diagnosis of endometriosis. However, at present BDNF can only be measured by ELISA which requires a clinic visit, a routine blood sample, and laboratory testing. Therefore, we developed a point-of-care device (EndoChip) for use with small blood volumes that can be collected through a finger prick...
June 22, 2018: Analytical Chemistry
Paulo Ricardo Protachevicz, Rafael Ribaski Borges, Adriane da Silva Reis, Fernando da Silva Borges, Kelly Cristina Iarosz, Ibere Luiz Caldas, Ewandson Luiz Lameu, Elbert Einstein Nehrer Macau, Ricardo Luiz Viana, Igor M Sokolov, Fabiano A S Ferrari, Jürgen Kurths, Antonio Marcos Batista
We consider a network topology according to the cortico-cortical connec-
 tion network of the human brain, where each cortical area is composed of a random
 network of adaptive exponential integrate-and-fire neurons. Depending on the
 parameters, this neuron model can exhibit spike or burst patterns. As a diagnostic
 tool to identify spike and burst patterns we utilise the coefficient of variation of the
 neuronal inter-spike interval. In our neuronal network, we verify the existence of spike
 and burst synchronisation in different cortical areas...
June 22, 2018: Physiological Measurement
Patrizia Ferroni, Piero Barbanti, Antonella Spila, Federica Fratangeli, Cinzia Aurilia, Luisa Fofi, Gabriella Egeo, Fiorella Guadagni
Background Migraine is the most common neurological disorder and the second most disabling human condition, whose pathogenesis is favored by a combination of genetic, epigenetic, and environmental factors. In recent years, several efforts have been made to identify reliable biomarker(s) useful to monitor disease activity and/or ascertain the response to a specific treatment. Objective To review the current evidence on the potential biological markers associated with migraine. Methods A structured search of peer-reviewed research literature was performed by searching major publications databases up to December 2017...
June 22, 2018: Current Medicinal Chemistry
Ping Hu, Shengjian Zhang, Tong Wu, Dalong Ni, Wenpei Fan, Yan Zhu, Rong Qian, Jianlin Shi
Effectiveness of cancer therapy relies heavily on the efficient early diagnosis. Circulating tumor DNA (ctDNA) detection is one of the most clinically meaningful liquid biopsy approaches for the noninvasive cancer early diagnosis, which, unfortunately, cannot be applied as a routine diagnostic tool till a number of obstacles, for example, unsatisfactory specificity and sensitivity, and extremely high costs, are overcome. Here, the first paradigm of nanomaterial's application in the extremely specific, ultrasensitive, and yet economical ctDNA detections is reported based on a facile nanoparticle-coupling strategy without amplification, with which polymerase chain reaction (PCR)-introduced bias and other shortcomings are successfully circumvented...
June 22, 2018: Advanced Materials
Daniele Orso, Alessio Ban, Nicola Guglielmo
PURPOSE: Pneumonia is the third leading cause of death in children under 5 years of age worldwide. In pediatrics, both the accuracy and safety of diagnostic tools are important. Lung ultrasound (LUS) could be a safe diagnostic tool for this reason. We searched in the literature for diagnostic studies about LUS to predict pneumonia in pediatric patients using systematic review and meta-analysis. METHODS: The Medline, CINAHL, Cochrane Library, Embase, SPORTDiscus, ScienceDirect, and Web of Science databases from inception to September 2017 were searched...
June 21, 2018: Journal of Ultrasound
Michael M Segal, Alanna K Rahm, Nathan C Hulse, Grant Wood, Janet L Williams, Lynn Feldman, Gregory J Moore, David Gehrum, Michelle Yefko, Steven Mayernick, Roger Gildersleeve, Margie C Sunderland, Steven B Bleyl, Peter Haug, Marc S Williams
Introduction: Reducing misdiagnosis has long been a goal of medical informatics. Current thinking has focused on achieving this goal by integrating diagnostic decision support into electronic health records. Methods: A diagnostic decision support system already in clinical use was integrated into electronic health record systems at two large health systems, after clinician input on desired capabilities. The decision support provided three outputs: editable text for use in a clinical note, a summary including the suggested differential diagnosis with a graphical representation of probability, and a list of pertinent positive and pertinent negative findings (with onsets)...
December 6, 2017: EGEMS
Renuka Sriram, Julia Nguyen, Justin DeLos Santos, Linda Nguyen, Jinny Sun, Seth Vigneron, Mark Van Criekinge, John Kurhanewicz, John D MacKenzie
The detection and treatment monitoring of inflammatory states remain challenging in part due to the multifactorial mechanisms of immune activation and spectrum of clinical manifestations. Currently, diagnostic strategies tend to be subjective and limited quantitative tools exist to monitor optimal treatment strategies. Pro-inflammatory M1 polarized macrophages exhibit a distinct metabolic glycolytic phenotype compared to the continuum of M2 polarization states. In the present study, the distinct metabolic phenotypes of resting and activated macrophages were successfully characterized and quantified using hyperpolarized carbon-13 (13 C) labeled pyruvate and its metabolic products, i...
2018: Theranostics
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"