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https://www.readbyqxmd.com/read/29141798/ecotropic-viral-integration-site-5-evi5-variants-are-associated-with-multiple-sclerosis-in-iranian-population
#1
Mehrdokht Mazdeh, Soudeh Ghafouri-Fard, Rezvan Noroozi, Arezou Sayad, Maryam Khani, Mohammad Taheri, Mir Davood Omrani
BACKGROUND: Multiple sclerosis (MS) is a multifactorial disorder with immunological basis. Numerous genetic and environmental factors contribute in its pathogenesis. Several genetic loci have been shown to be associated with MS risk. Among genes whose participation in MS has been evaluated is Ecotropic Viral Integration Site 5 (EVI5). EVI5 is a common site of retroviral integration with a possible role in T-cell lymphomagenesis. METHODS: In the current study, we aimed to confirm association of the single nucleotide polymorphisms (SNPs) within EVI5 gene with MS in 410 relapsing-remitting MS patients and 410 controls from Iranian population...
November 2017: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/29141248/association-of-functional-genetic-variants-of-hotair-with-hepatocellular-carcinoma-hcc-susceptibility-in-a-chinese-population
#2
Hao Li, Xian-Mei Tang, Yangchen Liu, Weizhen Li, Qiaoyun Chen, Yan Pan
BACKGROUND/AIMS: The HOX transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), plays an important role in the pathogenesis and progression of multiple tumors. The aim of the present study was to evaluate whether common single nucleotide polymorphisms (SNPs) in HOTAIR are related to hepatocellular carcinoma (HCC) susceptibility in a Chinese population. METHODS: We genotyped three SNPs of HOTAIR in a hepatocellular carcinoma (HCC) case-control study, including 482 cases and 520 control subjects...
November 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29139543/molecular-cloning-and-expression-analysis-of-sox3-during-gonad-and-embryonic-development-in-misgurnus-anguillicaudatus
#3
Xiaohua Xia, Weiran Huo, Ruyan Wan, Linxia Zhang, Xiaopei Xia, Zhongjie Chang
Sox3 is a single-exon gene located on the X chromosome in most vertebrates. It belongs to the SoxB1 subfamily, which is part of the larger Sox family. Previous studies have revealed that Sox3 is expressed in many fish species. However, how Sox3 influences the development of Misgurnus anguillicaudatus remains unknown. In this study, a Sox3 homologue, termed MaSox3, was cloned from the brain of M. anguillicaudatus using homology-based cloning and the rapid amplification of cDNA ends method. Sequence analysis reveals that MaSox3 encodes a hydrophilic protein, which contains a characteristic HMG-box DNA-binding domain of 79 amino acids, and shares high homology with Sox3 in other species...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/29139141/developing-novel-species-specific-dna-markers-for-pcr-based-species-identification-of-the-lactobacillus-sakei-group
#4
Chien-Hsun Huang, Jong-Shian Liou, Lina Huang, Koichi Watanabe
Identification members of the Lactobacillus sakei group (LSG) by common phenotypic and genotypic methods is generally inadequate and time-consuming. The objective of this study was to develop novel species-specific primers based on sequence-characterized amplified region (SCAR) markers using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. Three species-specific fragments were gel-purified, cloned, and sequenced after preliminary screening of 80 random primers. Accordingly, three pairs of primers Lcur-F/R, Lgram-F/R, and Lsakei-F/R were designed based on single species-specific bands (281, 278, and 472 bp) that were obtained from L...
November 14, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29136137/copy-number-variation-and-expression-analysis-reveals-a-non-orthologous-pinta-gene-family-member-involved-in-butterfly-vision
#5
Aide Macias-Muñoz, Kyle J McCulloch, Adriana D Briscoe
Vertebrate (CRALBP) and Drosophila (PINTA) proteins with a CRAL-TRIO domain transport retinal-based chromophores that bind to opsin proteins and are necessary for phototransduction. The CRAL-TRIO domain gene family is composed of genes that encode proteins with a common N-terminal structural domain. While there is an expansion of this gene family in Lepidoptera, there is no lepidopteran ortholog of pinta. Further, the function of these genes in lepidopterans has not yet been established. Here we explored the molecular evolution and expression of CRAL-TRIO domain genes in the butterfly Heliconius melpomene in order to identify a member of this gene family as a candidate chromophore transporter...
November 9, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29135943/the-effects-of-4-esterified-resveratrol-derivatives-on-calcium-dynamics-in-breast-cancer-cells
#6
Joshua A Peterson, Hayden P Doughty, Austin J Eells, Trent A Johnson, Jordan P Hastings, Colton M Crowther, Merritt B Andrus, Jason D Kenealey
Triple-negative breast cancer is a highly aggressive subtype of breast cancer. Frequently, breast cancer cells modulate their calcium signaling pathways to optimize growth. Unique calcium pathways in breast cancer cells could serve as a way to target tumorigenic cells without affecting normal tissue. Resveratrol has previously been shown to activate calcium signaling pathways. We use cell viability, single-cell calcium microscopy, and RT-PCR assays to determine the activity and mechanism of three different 4'-esterified resveratrol derivatives...
November 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29133054/synthesis-crystal-structure-and-biological-evaluation-of-a-new-dasatinib-copper-ii-complex-as-telomerase-inhibitor
#7
Qi-Pin Qin, Ting Meng, Ming-Xiong Tan, Yan-Cheng Liu, Xu-Jian Luo, Bi-Qun Zou, Hong Liang
A new copper(II) complex of dasatinib (DAS) was synthesized and characterized via ESI-MS, UV-Vis, IR, single-crystal X-ray diffraction analysis, (1)H and (13)C NMR spectroscopy, and elemental analysis. The composition of the new complex (1) was found to be [Cu(DAS + H)(NO3)2(H2O)]NO3·(H2O)·(CH3OH). Through MTT assay, it was found that 1 had high cytotoxicity towards A549, HeLa, BEL-7402, Hep-G2, NCI-H460, and MGC80-3 tumor cell lines, with IC50 values in 4.04-13.04 μM. The Hep-G2 cells were the most sensitive to 1...
October 25, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29132961/title-experimental-asthma-persists-in-il33-receptor-knockout-mice-due-to-the-emergence-of-a-tslp-driven-il9-and-il13-ilc2-subpopulations
#8
Mukesh Verma, Sucai Liu, Lidia Michalec, Anand Sripada, Magdalena M Gorska, Rafeul Alam
BACKGROUND: IL33 plays an important role in development of experimental asthma. OBJECTIVE: To study the role of the IL33 receptor (ST2) in persistence of asthma in a mouse model. METHODS: We studied allergen-induced experimental asthma in ST2 knockout (KO) and wild-type control mice. We measured airway hyperresponsiveness (AHR) by flexivent, inflammatory indices by ELISA, histology and real-time PCR, and ILC2s in lung single cell preparations by flow cytometry...
November 10, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29131838/expanded-repertoire-of-kinetoplast-associated-proteins-and-unique-mitochondrial-dna-arrangement-of-symbiont-bearing-trypanosomatids
#9
Silvana Sant Anna de Souza, Carolina Moura Catta-Preta, João Marcelo P Alves, Danielle P Cavalcanti, Marta M G Teixeira, Erney P Camargo, Wanderley De Souza, Rosane Silva, Maria Cristina M Motta
In trypanosomatids, the kinetoplast is the portion of the single mitochondrion that is connected to the basal body and contains the kDNA, a network composed by circular and interlocked DNA. The kDNA packing is conducted by Kinetoplast Associated Proteins (KAPs), which are similar to eukaryotic histone H1. In symbiont-harboring trypanosomatids (SHTs) such as Angomonas deanei and Strigomonas culicis, a ß-proteobacterium co-evolves with the host in a mutualistic relationship. The prokaryote confers nutritional benefits to the host and affects its cell structure...
2017: PloS One
https://www.readbyqxmd.com/read/29131699/genotype-specific-minimal-residual-disease-interpretation-improves-stratification-in-pediatric-acute-lymphoblastic-leukemia
#10
David O'Connor, Amir Enshaei, Jack Bartram, Jeremy Hancock, Christine J Harrison, Rachael Hough, Sujith Samarasinghe, Claire Schwab, Ajay Vora, Rachel Wade, John Moppett, Anthony V Moorman, Nick Goulden
Purpose Minimal residual disease (MRD) and genetic abnormalities are important risk factors for outcome in acute lymphoblastic leukemia. Current risk algorithms dichotomize MRD data and do not assimilate genetics when assigning MRD risk, which reduces predictive accuracy. The aim of our study was to exploit the full power of MRD by examining it as a continuous variable and to integrate it with genetics. Patients and Methods We used a population-based cohort of 3,113 patients who were treated in UKALL2003, with a median follow-up of 7 years...
November 13, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/29128690/transcriptome-based-biological-dosimetry-of-gamma-radiation-in-arabidopsis-using-dna-damage-response-genes
#11
Tae Ho Ryu, Jin Kyu Kim, Jeong-Il Kim, Jin-Hong Kim
Plants are used as representative reference biota for the biological assessment of environmental risks such as ionizing radiation due to their immobility. This study proposed a faster, more economical, and more effective method than conventional cytogenetic methods for the biological dosimetry of ionizing radiation in plants (phytodosimetry). We compared various dose-response curves for the radiation-induced DNA damage response (DDR) in Arabidopsis thaliana after relatively "low-dose" gamma irradiation (3, 6, 12, 24, and 48 Gy) below tens of Gy using comet (or single-cell gel electrophoresis), gamma-H2AX, and transcriptomic assays of seven DDR genes (AGO2, BRCA1, GRG, PARP1, RAD17, RAD51, and RPA1E) using quantitative real time PCR...
November 9, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/29128610/changes-in-redox-regulation-during-transition-from-c3-to-single-cell-c4-photosynthesis-in-bienertia-sinuspersici
#12
Baris Uzilday, Rengin Ozgur, Tolga Yalcinkaya, Ismail Turkan, A Hediye Sekmen
Bienertia sinuspersici performs single cell C4 photosynthesis without Kranz anatomy. Peripheral and central cytoplasmic compartments in a single chlorenchyma cell act as mesophyll cells and bundle sheath cells. Development of this specialized mechanism is gradual during plant development. Young leaves perform C3 photosynthesis, while mature leaves have complete C4 cycle. The aim of this work was to investigate changes in redox regulation and antioxidant defence during transition from C3 to single cell C4 photosynthesis in B...
October 31, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29126924/suppression-of-p53r2-gene-expression-with-specific-sirna-sensitizes-hepg2-cells-to-doxorubicin
#13
Ako Azimi, Maryam Majidinia, Vahid Shafiei-Irannejad, Rana Jahanban-Esfahlan, Yasin Ahmadi, Ansar Karimian, Seyed Mostafa Mir, Hadi Karami, Bahman Yousefi
INTRODUCTION: p53R2 is a p53-inducible protein that contributes to DNA repair by providing dNTPs in response to DNA damage. The roles of p53R2 in cancer cells and malignancies still remain controversial. Herein, we examined the effects of p53R2 silencing on HepG2 human hepatocellular carcinoma (HHC) cell line (wild-type p53) viability, apoptosis and cell cycle arrest in the presence and absence of doxorubicin. METHODS: Cell transfection was performed using a liposomal approach...
November 7, 2017: Gene
https://www.readbyqxmd.com/read/29121914/splice-variants-of-the-extracellular-region-of-ron-receptor-tyrosine-kinase-in-lung-cancer-cell-lines-identified-by-pcr-and-sequencing
#14
Soundararajan Krishnaswamy, Abdul Khader Mohammed, Gyanendra Tripathi, Majed S Alokail, Nasser M Al-Daghri
BACKGROUND: Altered expression of receptor tyrosine kinases (RTKs) is a major driver of growth and metastasis of cancers. Recepteur d'origine nantais (RON) receptor is a single-pass transmembrane RTK aberrantly expressed in a number of cancers. Efforts to block deregulated RON signaling in tumors using small molecule kinase inhibitors or antibodies are complicated by the presence of unknown number/types of isoforms of RON, which, despite having similar sequences, are localized differently and mediate varied functions...
November 9, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29120463/compartmentalized-partnered-replication-for-the-directed-evolution-of-genetic-parts-and-circuits
#15
Zhanar Abil, Jared W Ellefson, Jimmy D Gollihar, Ella Watkins, Andrew D Ellington
Compartmentalized partnered replication (CPR) is an emulsion-based directed evolution method based on a robust and modular phenotype-genotype linkage. In contrast to other in vivo directed evolution approaches, CPR largely mitigates host fitness effects due to a relatively short expression time of the gene of interest. CPR is based on gene circuits in which the selection of a 'partner' function from a library leads to the production of a thermostable polymerase. After library preparation, bacteria produce partner proteins that can potentially lead to enhancement of transcription, translation, gene regulation, and other aspects of cellular metabolism that reinforce thermostable polymerase production...
December 2017: Nature Protocols
https://www.readbyqxmd.com/read/29118738/monitoring-of-dual-crispr-cas9-mediated-steroidogenic-acute-regulatory-protein-gene-deletion-and-cholesterol-accumulation-using-high-resolution-fluorescence-in-situ-hybridization-in-a-single-cell
#16
Jinwoo Lee, Colin Jefcoate
Recent advances in fluorescence microscopy, coupled with CRISPR/Cas9 gene editing technology, provide opportunities for understanding gene regulation at the single-cell level. The application of direct imaging shown here provides an in situ side-by-side comparison of CRISPR/Cas9-edited cells and adjacent unedited cells. We apply this methodology to the steroidogenic acute regulatory protein (StAR) gene in Y-1 adrenal cells and MA-10 testis cells. StAR is a gatekeeper protein that controls the access of cholesterol from the cytoplasm to the inner mitochondria...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29118466/associations-of-functional-microrna-binding-site-polymorphisms-in-il23-th17-inflammatory-pathway-genes-with-gastric-cancer-risk
#17
Kaiyan Dong, Yajuan Xu, Qian Yang, Jiachen Shi, Jicheng Jiang, Yi Chen, Chunhua Song, Kaijuan Wang
IL23/Th17 axis acts as an inflammatory pathway in gastric carcinogenesis. MicroRNA- (miRNA-) binding site single-nucleotide polymorphisms (SNPs) of inflammatory genes may alter gastric cancer (GC) susceptibility. In this study, four miRNA binding site SNPs (rs3748067 of IL17A, rs887796, rs1468488 of IL17RA, and rs10889677 of IL23R) were genotyped from 500 patients and 500 controls. Unconditional logistic regression analyses and multifactor dimensionality reduction software were used to evaluate the relationships of SNPs with GC and gene-environment interactions, respectively...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/29116520/next-generation-dna-sequencing-of-vh-vl-repertoires-a-primer-and-guide-to-applications-in-single-domain-antibody-discovery
#18
Kevin A Henry
Immunogenetic analyses of expressed antibody repertoires are becoming increasingly common experimental investigations and are critical to furthering our understanding of autoimmunity, infectious disease, and cancer. Next-generation DNA sequencing (NGS) technologies have now made it possible to interrogate antibody repertoires to unprecedented depths, typically by sequencing of cDNAs encoding immunoglobulin variable domains. In this chapter, we describe simple, fast, and reliable methods for producing and sequencing multiplex PCR amplicons derived from the variable regions (VH, VHH or VL) of rearranged immunoglobulin heavy and light chain genes using the Illumina MiSeq platform...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29116065/-immunogenicity-of-recombinant-analog-of-antitumor-protein-lactaptin
#19
A V Tkachenko, O S Troitskaya, D V Semenov, E V Dmitrienko, E V Kuligina, V A Richter, O A Koval
Therapeutic monoclonal antibodies and recombinant proteins including cytokines are commonly used in the treatment of cancer and inflammatory diseases. In most cases, these protein-based drugs exhibit a high therapeutic efficacy, which is unfortunately frequently associated with a variety of side effects. We have investigated the in vitro and in vivo immunogenicity of recombinant antitumor protein lactaptin (RL2). Based on the qRT-PCR analysis, we have shown that, in MDA-MB-231 human breast adenocarcinoma cells, RL2 suppresses the NF-kB signaling cascade that regulates the reactions of innate immunity...
September 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/29115408/salidroside-could-enhance-the-cytotoxic-effect-of-l%C3%A2-ohp-on-colorectal-cancer-cells
#20
Xiaoming Shi, Wei Zhao, Yongbin Yang, Shengchun Wu, Bonan Lv
Evidence has suggested that salidroside inhibits the proliferation and invasion of renal clear cell, lung, breast, and colon cancer. However, effect of salidroside on colorectal cancer (CRC) cells against oxaliplatin (L‑OHP) resistance remains unclear. In the present study, the CRC HT‑29 cell line and L‑OHP resistance HT‑29/L‑OHP cell line were used to evaluate the effect, and mechanism of salidroside on L‑OHP resistance. The results demonstrated that the activity of HT‑29 cells was lower compared with that of HT‑29/L‑OHP cells following L‑OHP intervention, and was accompanied with varied expression levels of drug resistant proteins...
October 20, 2017: Molecular Medicine Reports
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