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platelet clump

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https://www.readbyqxmd.com/read/28337761/depression-and-cognitive-deficits-as-long-term-consequences-of-thrombotic-thrombocytopenic-purpura
#1
Tanja Falter, Veronique Schmitt, Stephanie Herold, Veronika Weyer, Charis von Auer, Stefanie Wagner, Gudrun Hefner, Manfred Beutel, Karl Lackner, Bernhard Lämmle, Inge Scharrer
BACKGROUND: Thrombotic thrombocytopenic purpura (TTP) is an acute life-threatening microangiopathy with a tendency of relapse characterized by consumptive thrombocytopenia, microangiopathic hemolytic anemia, and spontaneous von Willebrand factor-induced platelet clumping leading to microthrombi. The brain is frequently affected by microthrombi leading to neurologic abnormalities of varying severity. STUDY DESIGN AND METHODS: The aim of this observational cohort study was to investigate the prevalence of depression and cognitive deficits in 104 patients having survived acute TTP...
May 2017: Transfusion
https://www.readbyqxmd.com/read/28319015/collection-storage-inspection-and-quality-control-of-platelet-concentrates-obtained-by-apheresis-the-situation-in-spain
#2
Azucena Castrillo, Teresa Jimenez-Marco, José L Arroyo, María L Jurado, Luis Larrea, Rosa M Maymo, Jorge Monge, Carmen Muñoz, Ángel Pajares, Marta Yáñez
BACKGROUND: Diverse variables are involved in apheresis platelet collection, processing and storage. This survey shows how these are realized in Spain. METHOD: An analysis of collected data was performed in a questionnaire completed by ten Transfusion Centers (TC) which perform between 50 and 520 apheresis procedures per month. This information comprises the procedures used to collect, inspect and store apheresis platelet concentrates (PC), and quality control data...
February 22, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28150319/factors-influencing-platelet-clumping-during-peripheral-blood-hematopoietic-stem-cell-collection
#3
Gagan Mathur, Sarah L Mott, Laura Collins, Gail A Nelson, C Michael Knudson, Annette J Schlueter
BACKGROUND: Platelet clumping is a common occurrence during peripheral blood hematopoietic stem cell (HSC) collection using the Spectra Optia mononuclear cell (MNC) protocol. If clumping persists, it may prevent continuation of the collection and interfere with proper MNC separation. This study is the first to report the incidence of clumping, identify precollection factors associated with platelet clumping, and describe the degree to which platelet clumping interferes with HSC product yield...
May 2017: Transfusion
https://www.readbyqxmd.com/read/28060120/type-2b-von-willebrand-disease-an-unusual-cause-of-severe-neonatal-thrombocytopenia
#4
Seth J Rotz, Joseph S Palumbo, Russell E Ware
An infant with presumed maternal immune thrombocytopenic purpura had persistent thrombocytopenia with platelet clumping. The patient had no significant bleeding symptoms in the first year of life and von Willebrand antigen and ristocetin cofactor activity were normal. Absent high molecular weight multimers ultimately led to a genetically proven diagnosis of type 2B von Willebrand disease (3964G>A VWF exon 28), highlighting the challenges of establishing this diagnosis in infants.
January 5, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28034522/comparison-of-two-methodologies-for-the-enrichment-of-mononuclear-cells-from-thawed-cord-blood-products-the-automated-sepax-system-versus-the-manual-ficoll-method
#5
Indreshpal Kaur, Jane M Zulovich, Marissa Gonzalez, Kara M McGee, Nirmali Ponweera, Daljit Thandi, Enrique F Alvarez, Kathy Annandale, Frank Flagge, Katayoun Rezvani, Elizabeth Shpall
BACKGROUND AIMS: Umbilical cord blood (CB) is being used as a source of hematopoietic stem cells (HSCs) and immune cells to treat many disorders. Because these cells are present in low numbers in CB, investigators have developed strategies to expand HSCs and other immune cells such as natural killer (NK) cells. The initial step in this process is to enrich mononuclear cells (MNCs) while depleting unwanted cells. The manual method of MNC enrichment is routinely used by many centers; however, it is an open system, time-consuming and operator dependent...
December 26, 2016: Cytotherapy
https://www.readbyqxmd.com/read/27830476/production-of-heterozygous-alpha-1-3-galactosyltransferase-ggta1-knock-out-transgenic-miniature-pigs-expressing-human-cd39
#6
Kimyung Choi, Joohyun Shim, Nayoung Ko, Heejong Eom, Jiho Kim, Jeong-Woong Lee, Dong-Il Jin, Hyunil Kim
Production of transgenic pigs for use as xenotransplant donors is a solution to the severe shortage of human organs for transplantation. The first barrier to successful xenotransplantation is hyperacute rejection, a rapid, massive humoral immune response directed against the pig carbohydrate GGTA1 epitope. Platelet activation, adherence, and clumping, all major features of thrombotic microangiopathy, are inevitable results of immune-mediated transplant rejection. Human CD39 rapidly hydrolyzes ATP and ADP to AMP; AMP is hydrolyzed by ecto-5'-nucleotidase (CD73) to adenosine, an anti-thrombotic and cardiovascular protective mediator...
November 9, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27751864/evaluation-of-the-lh780-hematology-analyzer-for-detection-and-therapeutic-monitoring-of-malaria-cross-reactivity-with-nucleated-rbcs
#7
Jung Yoon, Seung Gyu Yun, Jeeyong Kim, Yoo Na Jung, Young Eun Koh, Jeong Hun Nam, Eun Taek Han, Chae Seung Lim
We evaluated the diagnostic usefulness of the LH780 Coulter blood cell counter for detection and therapeutic monitoring of malaria including cross-reactivity with nucleated RBC (NRBC) samples. A total of 405 patients (43 patients with Plasmodium vivax malaria and the control group of 120 healthy subjects, 111 patients with fever, and 131 patients with NRBCs) were analyzed with routine CBC using the LH780. We analyzed the CBC results according to three selected parameters: an abnormal peak in the WBC histogram before 35fL, the presence of red dots in the nonwhite cell zone of 2D WBC Diff Dataplot, and platelet-related flags suggesting platelet clumps or giant platelets...
December 2016: Acta Tropica
https://www.readbyqxmd.com/read/27676234/comparative-evaluation-of-a-heparin-citrate-anticoagulation-for-ldl-apheresis-in-two-primary-apheresis-systems
#8
Doris Handschel, Michiel Etienne Janssens, Marion Gericke, Stef De Reys, Helmut Borberg
BACKGROUND: As COBE Spectra has been replaced in many parts of the world, we describe a new protocol for low-density lipoprotein (LDL)-apheresis performed on familial hypercholesterolemia patients for the Spectra Optia platform. METHODS: For all procedures, after administering a bolus of heparin of 2,500 U, 10,000 U of heparin added to a 600 ml ACD-A bag was used as anticoagulant (AC). In a first phase (A), 16 apheresis procedures with COBE Spectra using an inlet:AC ratio of 25:1 were compared to 18 LDL-apheresis treatments with Spectra Optia at split Inlet:AC ratios of 16:1/18:1 or 20:1/25:1...
September 27, 2016: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/27634983/the-necessity-of-using-heparin-in-the-ultratag-rbc-kit-when-tagging-blood-for-a-nuclear-medicine-study
#9
Sarah R Pigmon, Kara D Weatherman, Nicholas C Brehl, Cybil J Nielsen
The purpose of this research was to evaluate the need to use heparin when preparing an UltraTag red blood cell (RBC) kit for a nuclear medicine study. METHODS: Nonheparinized blood samples (n = 15) and heparinized blood samples (n = 15) were added to UltraTag RBC kits. The samples were examined for macroscopic blood clotting and microscopic platelet clumping. As a control, samples with heparin (n = 15) and without heparin (n = 15) were used to help evaluate the effectiveness of the anticoagulant properties within the UltraTag RBC kit (sodium citrate) and whether those properties played a role in preventing clots or clumps...
December 2016: Journal of Nuclear Medicine Technology
https://www.readbyqxmd.com/read/27391303/effect-of-prewarming-edta-blood-samples-to-37%C3%A2-c-on-platelet-count-measured-by-sysmex-xt-2000iv-in-dogs-cats-and-horses
#10
Tim L Williams, Joy Archer
BACKGROUND: Pseudothrombocytopenia secondary to platelet clumping is a common cause of preanalytic error for platelet counts in dogs, cats, and horses. In human beings, it is suggested that prewarming blood samples to 37°C prior to hematology analysis will reduce platelet clumping. OBJECTIVES: The purpose of the study was to evaluate the effect of prewarming EDTA blood samples to 37°C on measured platelet counts and other hematologic variables. METHODS: The EDTA blood samples from dogs, cats and horses submitted to the clinical pathology laboratory at the University of Cambridge were included...
September 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27336691/contribution-of-staphylococcus-aureus-coagulases-and-clumping-factor-a-to-abscess-formation-in-a-rabbit-model-of-skin-and-soft-tissue-infection
#11
Natalia Malachowa, Scott D Kobayashi, Adeline R Porter, Kevin R Braughton, Dana P Scott, Donald J Gardner, Dominique M Missiakas, Olaf Schneewind, Frank R DeLeo
Staphylococcus aureus produces numerous factors that facilitate survival in the human host. S. aureus coagulase (Coa) and von Willebrand factor-binding protein (vWbp) are known to clot plasma through activation of prothrombin and conversion of fibrinogen to fibrin. In addition, S. aureus clumping factor A (ClfA) binds fibrinogen and contributes to platelet aggregation via a fibrinogen- or complement-dependent mechanism. Here, we evaluated the contribution of Coa, vWbp and ClfA to S. aureus pathogenesis in a rabbit model of skin and soft tissue infection...
2016: PloS One
https://www.readbyqxmd.com/read/27148840/type-2b-von-willebrand-disease-a-matter-of-plasma-plus-platelet-abnormality
#12
REVIEW
Giancarlo Castaman, Augusto B Federici
Type 2B von Willebrand disease (VWD2B) is a rare, autosomal-dominant inherited bleeding disorder, characterized by an enhanced ristocetin-induced platelet aggregation in platelet-rich plasma and often with variable degree of thrombocytopenia and loss of high-molecular-weight multimers von Willebrand factor (VWF). All these phenomena are caused by a mutant VWF, normally synthesized and assembled by endothelial cells, but with heightened affinity binding to the platelet receptor glycoprotein Ib-α (GpIb-α). When this abnormal VWF is released into the circulation and under specific clinical circumstances, in vivo platelet clumping is observed...
July 2016: Seminars in Thrombosis and Hemostasis
https://www.readbyqxmd.com/read/27114250/assessment-of-the-reliability-of-the-sysmex-xe-5000-analyzer-to-detect-platelet-clumps
#13
Jennifer Hawkins, Gene Gulati, Guldeep Uppal, Jerald Gong
OBJECTIVE: Platelet counts generated by automated analyzers on blood specimens that contain platelet clumps are often inaccurate and require verification by blood-smear review. In this study, we assessed the reliability of the Sysmex XE-5000 instrument to detect platelet clumps. METHOD: We reviewed automated complete blood count (CBC) results and the findings of the microscopic review of corresponding blood smears of 600 blood specimens specifically selected from the routine laboratory workload...
August 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27027391/blood-vessel-occlusion-in-peri-burn-tissue-is-secondary-to-erythrocyte-aggregation-and-mitigated-by-a-fibronectin-derived-peptide-that-limits-burn-injury-progression
#14
Bilal Asif, Abdul Rahim, Justine Fenner, Fubao Lin, Douglas Hirth, John Hassani, Steven A McClain, Adam J Singer, Marcia G Tonnesen, Richard A F Clark
Although vascular occlusion has long been noted in peri-burn tissue, the literature is inconsistent regarding the nature of the occlusion, with articles in the 1940s claiming that erythrocytes were the culprit and in the 1980s-1990s that microthrombi were responsible. To better define the nature of vessel occlusion, we studied two porcine burn models, a hot comb horizontal injury model and a vertical injury progression model. In both cases, tissue from the first two days after burn were stained with hemotoxylin and eosin, or probed for platelets or for fibrinogen/fibrin...
May 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/26918669/alterations-due-to-dilution-and-anticoagulant-effects-in-hematologic-analysis-of-rodent-blood-samples-on-the-sysmex-xt-2000iv
#15
Kaitlin A Moorhead, Marielle L Discipulo, Jing Hu, Roberta C Moorhead, Jennifer L Johns
BACKGROUND: Clinical pathology of rodents is hindered by sample volume limitations. A single whole heparinized blood sample is often submitted for hematologic and clinical chemistry analysis in exploratory research settings, and sample dilution may be required. Published information on the potential impact of sample dilution and heparin use on hematology variables in rodents is sparse. OBJECTIVES: The purpose of the study was to evaluate the effects of sample dilution and of anticoagulant on hematologic analysis of mouse and rat blood samples on the Sysmex XT-2000iV...
June 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/26887655/comparison-of-different-anticoagulant-associations-on-haemostasis-and-biochemical-analyses-in-feline-blood-specimens
#16
Fanny Granat, Céline Monzali, Elisabeth Jeunesse, Maud Guerlin, Catherine Trumel, Anne Geffré, Nathalie Bourgès-Abella
OBJECTIVES: Universal anticoagulant could be an alternative to the multiple blood sampling required for clinical pathology investigations in cats. An association of citrate, theophylline, adenosine and dipyridamole (CTAD) has been reported to be a good substitute for EDTA for haematology analysis in cats, limiting platelet clumping, and has also been shown to be valid for haematology, secondary haemostasis and some biochemical variables in humans. The aim of the study was therefore to investigate the effects of CTAD on in vitro platelet aggregation and compare results of secondary haemostasis and biochemistry tests, excluding a priori those variables not reliably measured in CTAD, such as sodium, chloride and divalent cations, in feline blood specimens collected in CTAD and paired citrate and heparin tubes...
February 17, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/26749087/assessment-of-functional-fibrinolysis-in-cord-blood-using-modified-thromboelastography
#17
Rakefet Sidlik, Tzipora Strauss, Iris Morag, Boris Shenkman, Ilia Tamarin, Aharon Lubetsky, Tami Livnat, Gili Kenet
BACKGROUND: The fibrinolytic system in newborns is immature and probably impaired. The aim of this study was to prospectively evaluate functional fibrinolytic capacity of newborn's cord blood using a new thromboelastometry (rotational thromboelastogram, ROTEM®) test. METHODS: Infants born at Sheba Medical Center were studied prospectively. Cord blood was obtained immediately after clumping, and ROTEM parameters were assessed applying non-activated TEM (NATEM) assay with increasing concentration of tissue plasminogen activator (tPA, 0-200 U/ml)...
May 2016: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/26715615/sudden-development-of-thrombocytopenia-after-reversal-of-anticoagulation-for-surgery
#18
Mobeen Rahman, Ana Ortega-Lopez, Amy Powers
Herein, we report a case of post-transfusion purpura after the reversal of anticoagulation for surgical purposes in a 66-year old ethnic Asian man who was undergoing long-term warfarin therapy for antiphospholipid syndrome. The patient experienced a sudden decrease in platelet count, from 308,000 per μL from the day of admission to 38,000 per μL the following day. Follow-up testing revealed unremarkable red blood cell (RBC) morphology, no evidence of platelet clumping, and negative heparin-induced antibody test results...
February 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/26542105/study-on-the-kinetics-and-influence-of-feline-platelet-aggregation-and-deaggregation
#19
Barbara Riond, Andrea Katharina Waßmuth, Sonja Hartnack, Regina Hofmann-Lehmann, Hans Lutz
BACKGROUND: Feline platelets are prone to clumping after blood collection, rendering the determination of accurate platelet counts difficult for clinical laboratories and resulting in a high incidence of pseudothrombocytopenia in feline haematology reports. No information is available about the kinetics of platelet aggregate formation in feline ethylenediaminetetraacetic acid blood and the course of platelet counts over a clinically relevant time period. The aim of the present study was to determine platelet counts in healthy cats over a time period of 24 h after blood collection at 9 time points; to assess potential effects of platelet aggregates, anaesthesia and bleeding conditions on feline platelets and white blood cell counts; and finally, to investigate if glucose concentration is associated with the presence of aggregates...
2015: BMC Veterinary Research
https://www.readbyqxmd.com/read/26524955/-diagnostic-performance-of-graphical-anomalies-in-the-detection-of-large-platelets-and-platelet-clumps
#20
COMPARATIVE STUDY
M Sassi, W Dibej, B Abdi, F Abderrazak, M Hassine, H Babba
UNLABELLED: Thrombocytopenia is a current situation for making a blood smear in routine practice in a medical analysis laboratory. Recent automated hematology analyzers enumerate platelets and generate histograms and specific flags. Operators must be aware of the characteristics of their analyzer in order to avoid spurious results in the case where microscopy review is not possible. OBJECTIVE: We evaluated the diagnostic performance of various graphical anomalies in the detection of large platelets and platelet clumps...
December 2015: Pathologie-biologie
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