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platelet clump

Jung Yoon, Seung Gyu Yun, Jeeyong Kim, Yoo Na Jung, Young Eun Koh, Jeong Hun Nam, Eun Taek Han, Chae Seung Lim
We evaluated the diagnostic usefulness of the LH780 Coulter blood cell counter for detection and therapeutic monitoring of malaria including cross-reactivity with nucleated RBC (NRBC) samples. A total of 405 patients (43 patients with Plasmodium vivax malaria and the control group of 120 healthy subjects, 111 patients with fever, and 131 patients with NRBCs) were analyzed with routine CBC using the LH780. We analyzed the CBC results according to three selected parameters: an abnormal peak in the WBC histogram before 35fL, the presence of red dots in the nonwhite cell zone of 2D WBC Diff Dataplot, and platelet-related flags suggesting platelet clumps or giant platelets...
October 14, 2016: Acta Tropica
Doris Handschel, Michiel Etienne Janssens, Marion Gericke, Stef De Reys, Helmut Borberg
BACKGROUND: As COBE Spectra has been replaced in many parts of the world, we describe a new protocol for low-density lipoprotein (LDL)-apheresis performed on familial hypercholesterolemia patients for the Spectra Optia platform. METHODS: For all procedures, after administering a bolus of heparin of 2,500 U, 10,000 U of heparin added to a 600 ml ACD-A bag was used as anticoagulant (AC). In a first phase (A), 16 apheresis procedures with COBE Spectra using an inlet:AC ratio of 25:1 were compared to 18 LDL-apheresis treatments with Spectra Optia at split Inlet:AC ratios of 16:1/18:1 or 20:1/25:1...
September 27, 2016: Journal of Clinical Apheresis
Sarah R Pigmon, Kara D Weatherman, Nicholas Brehl, Cybil J Nielsen
: The purpose of this research is to evaluate the necessity of using heparin in UltraTag™ RBC kits used for nuclear medicine studies. METHODS: Non-heparinized blood (n = 15) and heparinized blood (n = 15) were added into UltraTag™ RBC kits. The samples were evaluated for macroscopic blood clots and microscopic platelet clumping. Control groups with heparin (n = 15) and control groups without heparin (n = 15) were used to help evaluate the effectiveness of the anticoagulant properties within the UltraTag™ RBC kits (sodium citrate) and to evaluate if that played a role in preventing blood clots/clumps...
September 15, 2016: Journal of Nuclear Medicine Technology
Tim L Williams, Joy Archer
BACKGROUND: Pseudothrombocytopenia secondary to platelet clumping is a common cause of preanalytic error for platelet counts in dogs, cats, and horses. In human beings, it is suggested that prewarming blood samples to 37°C prior to hematology analysis will reduce platelet clumping. OBJECTIVES: The purpose of the study was to evaluate the effect of prewarming EDTA blood samples to 37°C on measured platelet counts and other hematologic variables. METHODS: The EDTA blood samples from dogs, cats and horses submitted to the clinical pathology laboratory at the University of Cambridge were included...
September 2016: Veterinary Clinical Pathology
Natalia Malachowa, Scott D Kobayashi, Adeline R Porter, Kevin R Braughton, Dana P Scott, Donald J Gardner, Dominique M Missiakas, Olaf Schneewind, Frank R DeLeo
Staphylococcus aureus produces numerous factors that facilitate survival in the human host. S. aureus coagulase (Coa) and von Willebrand factor-binding protein (vWbp) are known to clot plasma through activation of prothrombin and conversion of fibrinogen to fibrin. In addition, S. aureus clumping factor A (ClfA) binds fibrinogen and contributes to platelet aggregation via a fibrinogen- or complement-dependent mechanism. Here, we evaluated the contribution of Coa, vWbp and ClfA to S. aureus pathogenesis in a rabbit model of skin and soft tissue infection...
2016: PloS One
Giancarlo Castaman, Augusto B Federici
Type 2B von Willebrand disease (VWD2B) is a rare, autosomal-dominant inherited bleeding disorder, characterized by an enhanced ristocetin-induced platelet aggregation in platelet-rich plasma and often with variable degree of thrombocytopenia and loss of high-molecular-weight multimers von Willebrand factor (VWF). All these phenomena are caused by a mutant VWF, normally synthesized and assembled by endothelial cells, but with heightened affinity binding to the platelet receptor glycoprotein Ib-α (GpIb-α). When this abnormal VWF is released into the circulation and under specific clinical circumstances, in vivo platelet clumping is observed...
July 2016: Seminars in Thrombosis and Hemostasis
Jennifer Hawkins, Gene Gulati, Guldeep Uppal, Jerald Gong
OBJECTIVE: Platelet counts generated by automated analyzers on blood specimens that contain platelet clumps are often inaccurate and require verification by blood-smear review. In this study, we assessed the reliability of the Sysmex XE-5000 instrument to detect platelet clumps. METHOD: We reviewed automated complete blood count (CBC) results and the findings of the microscopic review of corresponding blood smears of 600 blood specimens specifically selected from the routine laboratory workload...
August 2016: Laboratory Medicine
Bilal Asif, Abdul Rahim, Justine Fenner, Fubao Lin, Douglas Hirth, John Hassani, Steven A McClain, Adam J Singer, Marcia G Tonnesen, Richard A F Clark
Although vascular occlusion has long been noted in peri-burn tissue, the literature is inconsistent regarding the nature of the occlusion, with articles in the 1940s claiming that erythrocytes were the culprit and in the 1980s-1990s that microthrombi were responsible. To better define the nature of vessel occlusion, we studied two porcine burn models, a hot comb horizontal injury model and a vertical injury progression model. In both cases, tissue from the first two days after burn were stained with hemotoxylin and eosin, or probed for platelets or for fibrinogen/fibrin...
May 2016: Wound Repair and Regeneration
Kaitlin A Moorhead, Marielle L Discipulo, Jing Hu, Roberta C Moorhead, Jennifer L Johns
BACKGROUND: Clinical pathology of rodents is hindered by sample volume limitations. A single whole heparinized blood sample is often submitted for hematologic and clinical chemistry analysis in exploratory research settings, and sample dilution may be required. Published information on the potential impact of sample dilution and heparin use on hematology variables in rodents is sparse. OBJECTIVES: The purpose of the study was to evaluate the effects of sample dilution and of anticoagulant on hematologic analysis of mouse and rat blood samples on the Sysmex XT-2000iV...
June 2016: Veterinary Clinical Pathology
Fanny Granat, Céline Monzali, Elisabeth Jeunesse, Maud Guerlin, Catherine Trumel, Anne Geffré, Nathalie Bourgès-Abella
OBJECTIVES: Universal anticoagulant could be an alternative to the multiple blood sampling required for clinical pathology investigations in cats. An association of citrate, theophylline, adenosine and dipyridamole (CTAD) has been reported to be a good substitute for EDTA for haematology analysis in cats, limiting platelet clumping, and has also been shown to be valid for haematology, secondary haemostasis and some biochemical variables in humans. The aim of the study was therefore to investigate the effects of CTAD on in vitro platelet aggregation and compare results of secondary haemostasis and biochemistry tests, excluding a priori those variables not reliably measured in CTAD, such as sodium, chloride and divalent cations, in feline blood specimens collected in CTAD and paired citrate and heparin tubes...
February 17, 2016: Journal of Feline Medicine and Surgery
Rakefet Sidlik, Tzipora Strauss, Iris Morag, Boris Shenkman, Ilia Tamarin, Aharon Lubetsky, Tami Livnat, Gili Kenet
BACKGROUND: The fibrinolytic system in newborns is immature and probably impaired. The aim of this study was to prospectively evaluate functional fibrinolytic capacity of newborn's cord blood using a new thromboelastometry (rotational thromboelastogram, ROTEM®) test. METHODS: Infants born at Sheba Medical Center were studied prospectively. Cord blood was obtained immediately after clumping, and ROTEM parameters were assessed applying non-activated TEM (NATEM) assay with increasing concentration of tissue plasminogen activator (tPA, 0-200 U/ml)...
May 2016: Pediatric Blood & Cancer
Mobeen Rahman, Ana Ortega-Lopez, Amy Powers
Herein, we report a case of post-transfusion purpura after the reversal of anticoagulation for surgical purposes in a 66-year old ethnic Asian man who was undergoing long-term warfarin therapy for antiphospholipid syndrome. The patient experienced a sudden decrease in platelet count, from 308,000 per μL from the day of admission to 38,000 per μL the following day. Follow-up testing revealed unremarkable red blood cell (RBC) morphology, no evidence of platelet clumping, and negative heparin-induced antibody test results...
February 2016: Laboratory Medicine
Barbara Riond, Andrea Katharina Waßmuth, Sonja Hartnack, Regina Hofmann-Lehmann, Hans Lutz
BACKGROUND: Feline platelets are prone to clumping after blood collection, rendering the determination of accurate platelet counts difficult for clinical laboratories and resulting in a high incidence of pseudothrombocytopenia in feline haematology reports. No information is available about the kinetics of platelet aggregate formation in feline ethylenediaminetetraacetic acid blood and the course of platelet counts over a clinically relevant time period. The aim of the present study was to determine platelet counts in healthy cats over a time period of 24 h after blood collection at 9 time points; to assess potential effects of platelet aggregates, anaesthesia and bleeding conditions on feline platelets and white blood cell counts; and finally, to investigate if glucose concentration is associated with the presence of aggregates...
2015: BMC Veterinary Research
M Sassi, W Dibej, B Abdi, F Abderrazak, M Hassine, H Babba
UNLABELLED: Thrombocytopenia is a current situation for making a blood smear in routine practice in a medical analysis laboratory. Recent automated hematology analyzers enumerate platelets and generate histograms and specific flags. Operators must be aware of the characteristics of their analyzer in order to avoid spurious results in the case where microscopy review is not possible. OBJECTIVE: We evaluated the diagnostic performance of various graphical anomalies in the detection of large platelets and platelet clumps...
December 2015: Pathologie-biologie
Ingrid L Scully, Yekaterina Timofeyeva, David Keeney, Yury V Matsuka, Elena Severina, Lisa K McNeil, Jasdeep Nanra, George Hu, Paul A Liberator, Kathrin U Jansen, Annaliesa S Anderson
The Staphylococcus aureus virulence factor clumping factor A (ClfA) is a component of an investigational S. aureus prophylactic vaccine. ClfA enables S. aureus to bind to fibrinogen and platelets during the initial stages of invasive disease. Here we demonstrate that ectopic expression of ClfA is sufficient to render nonpathogenic Lactococcus lactis lethal in a murine model of systemic infection. In contrast, L. lactis expressing ClfAY338A, which cannot bind fibrinogen, did not cause death in the mice. Pathogenicity was also prevented by immunization with ClfA...
October 5, 2015: Vaccine
Óscar Fuster, Belinda Andino, Begoña Laiz
No abstract text is available yet for this article.
February 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Nancy Turner, Sarah Sartain, Joel Moake
The molecular linkage between ultralarge (UL) von Willebrand factor (VWF) multimers and the alternative complement pathway (AP) has recently been described. Endothelial cell (EC)-secreted and anchored ULVWF multimers (in long stringlike structures) function as both hyperadhesive sites that initiate platelet adhesion and aggregation and activating surfaces for the AP. In vitro, the active form of C3, C3b binds to the EC-anchored ULVWF multimeric strings and initiates the assembly on the strings of C3 convertase (C3bBb) and C5 convertase (C3bBbC3b)...
June 2015: Hematology/oncology Clinics of North America
Koji Miyazaki, Yukako Koike, Shinji Kunishima, Ryuji Ishii, Mikio Danbara, Ryouichi Horie, Yutaka Yatomi, Masaaki Higashihara
BACKGROUND: Reticulated platelets (RPs) as measured using flow cytometry are useful parameters of thrombopoiesis; however, difficulties remain with standardization between laboratories. On the other hand, immature platelet fraction (IPF) measurement, as determined using an automated hematology analyzer, is simple, reproducible, and displays a good correlation with RP, although specific factors may affect its value. We previously noticed that a small proportion of patients exhibit extremely high IPF values that do not correlate with flow cytometrically measured RP...
December 2015: Hematology (Amsterdam, Netherlands)
I M Sánchez Guiu, I Martínez-Martinez, C Martínez, J Navarro-Fernandez, F García-Candel, F Ferrer-Marín, V Vicente, S P Watson, R K Andrews, E E Gardiner, M L Lozano, J Rivera
Platelet cold agglutinins (PCA) cause pseudothrombocytopenia, spurious thrombocytopenia due to ex vivo platelet clumping, complicating clinical diagnosis, but mechanisms and consequences of PCA are not well defined. Here, we characterised an atypical immunoglobulin (Ig)M PCA in a 37-year-old woman with lifelong bleeding and chronic moderate thrombocytopenia, that induces activation and aggregation of autologous or allogeneic platelets via interaction with platelet glycoprotein (GP)VI. Patient temperature-dependent pseudothrombocytopenia was EDTA-independent, but was prevented by integrin αIIbβ3 blockade...
August 2015: Thrombosis and Haemostasis
Masahiro Kiso, Tatsuo S Hamazaki, Munenari Itoh, Sota Kikuchi, Hidemi Nakagawa, Hitoshi Okochi
BACKGROUND: The dermal papilla is composed of a small clump of mesenchymal cells, called dermal papilla cells (DPCs). DPCs closely interact with epidermal cells to give rise to hair follicles and shafts during hair follicle development and the hair cycle. DPCs are promising cell sources for hair regeneration therapy for alopecia patients. However, once DPCs are put into conventional two-dimensional culture conditions, they quickly lose their capability to produce hair follicles. OBJECTIVE: We aimed to expand a sufficiently large population of DPCs that retain their hair inductive activity...
August 2015: Journal of Dermatological Science
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