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https://www.readbyqxmd.com/read/28637006/the-effect-of-ca125-on-metastasis-of-ovarian-cancer-old-marker-new-function
#1
Qin Yuan, Jiayin Song, Weiwei Yang, Hongyan Wang, Qianyu Huo, Jie Yang, Xiaoxu Yu, Yunde Liu, Chen Xu, Huijing Bao
CA125 has been used extensively to screen for neoplasms, especially in ovarian cancer. The serum CA125 level can be used as a better prognosis evaluation and it may dynamic monitoring the disease progression. We explored the effect of CA125 on ovarian cancer cell migration and its underlying mechanism. Transwell assays showed that exposure to 0.2 μg/ml or 0.4 μg/ml CA125 for 48 h increased migration of A2780 and OVCAR-3 ovarian cancer cells. This effect of CA125 was blocked addition of 200 ng/ml DKK-1, a Wnt pathway inhibitor...
June 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28631525/polyphenol-profile-by-uhplc-ms-ms-anti-glycation-antioxidant-and-cytotoxic-activities-of-several-samples-of-propolis-from-the-northeastern-semi-arid-region-of-brazil
#2
Jadriane de Almeida Xavier, Iara Barros Valentim, Fabiana O S Camatari, Alberto M M de Almeida, Henrique Fonseca Goulart, Jamylle Nunes de Souza Ferro, Emiliano de Oliveira Barreto, Bruno Coelho Cavalcanti, Carla B G Bottoli, Marília Oliveira Fonseca Goulart
CONTEXT: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities. OBJECTIVE: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples. MATERIAL AND METHODS: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH(•), FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28626741/isolation-and-identification-of-two-phenolic-compounds-from-a-moderately-cytotoxic-fraction-of-cousinia-verbascifolia-bunge
#3
Seyed Ebrahim Sajjadi, Mustafa Ghanadian, Mehrangiz Haghighi
BACKGROUND: Little information is available about chemical components of the Cousinia genus. A primary cytotoxicity screening on Cousinia verbascifolia showed moderate cytotoxic activity against OVCAR-3 ovarian and HT-29 colon cancer cells. Therefore, the aim of this study is a phytochemical investigation to identify the compounds responsible for this bioactivity. MATERIALS AND METHODS: Extraction was done through percolation and fractionations by reverse phase column chromatography and normal column chromatography...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28560285/data-on-the-association-of-cmpk1-with-clinicopathological-features-and-biological-effect-in-human-epithelial-ovarian-cancer
#4
Daibing Zhou, Lingyun Zhang, Qunbo Lin, Weimin Ren, Guoxiong Xu
Human epithelial ovarian cancer (EOC) is the most lethal gynecological disease. However, the molecular mechanisms by which transforming growth factor-β (TGF-β) regulates ovarian tumor progression markers remain unclear. The present data show cytidine monophosphate kinase (CMPK) as an EOC biomarker and are related to the article entitled "Cytidine monophosphate kinase is inhibited by the TGF-β signalling pathway through the upregulation of miR-130b-3p in human epithelial ovarian cancer" [1]. CMPK, as well as cystatin B [2] and β-2-microglobulin [3], is overexpressed in human epithelial-type ovarian tumors...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28525848/an-amplified-comparative-fluorescence-resonance-energy-transfer-immunosensing-of-ca125-tumor-marker-and-ovarian-cancer-cells-using-green-and-economic-carbon-dots-for-bio-applications-in-labeling-imaging-and-sensing
#5
Somayeh Hamd-Ghadareh, Abdollah Salimi, Fardin Fathi, Saman Bahrami
CA125, is a marker in the clinical diagnosis of several cancers and currently is the best serum-based tumor marker for ovarian cancer. Here, we developed an ultrasensitive antibody-ssDNA aptamer sandwich-type fluorescence immunosensor for CA125 detection. Based on a novel signal amplification strategy the carbon dots (CDs) functionalized with aptamer (CD-aptamer) used as detection probe and PAMAM-Dendrimers/AuNPs was used for covalent attachment of CA125-antibody and completing the sandwich assay method. By measuring of fluorescence resonance energy transfer (FRET) signals between CDs and AuNPs as nanoquenchers, the fluorescence signal quenched during sandwich complex formed between anti-CA125, CA125 and CDs-Aptamer and decreasing of fluorescence response signal is related to CA125 concentrations...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28508842/a3-adenosine-receptor-agonist-induce-g1-cell-cycle-arrest-via-cyclin-d-and-cyclin-dependent-kinase-4-pathways-in-ovcar-3-and-caov-4-cell-lines
#6
Hamid Reza Joshaghani, Seyyed Mehdi Jafari, Mahmoud Aghaei, Mojtaba Panjehpour, Hamideh Abedi
AIM OF THE STUDY: The cell cycle, a vital process that involves in cells' growth and division, lies at the heart of cancer. It has been shown that IB-MECA, an A3 adenosine receptor agonist inhibits the proliferation of cancer cells by inducing cell cycle arrest in several tumors. In this study, we evaluated the role of IB-MECA inhibition in cell cycle progression in ovarian cancer cells. MATERIALS AND METHODS: Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in Caov-4 and OVCAR-3...
January 2017: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/28480832/synthesis-of-new-arylidene-2-5-diketopiperazines-and-evaluation-of-their-anti-acetylcholinesterase-anti-xanthine-oxidase-anti-diabetic-and-cytotoxic-activities
#7
Mohamed Amine Belkacem, Hichem Ben Jannet, Hicham Ferhout, Laila Mzali, Jalloul Bouajila
BACKGROUND: 2,5-diketopiperazine derivatives are considered to be important classes of cyclic peptides due to their wide range of biological activities. OBJECTIVES: Synthesis a new series of protected 2,5-diketopiperazine derivatives and evaluate their biological activities. METHODS: A series of new soluble mono-protected arylidene 2,5-diketopiperazine derivatives 3a-p has been prepared by application of Claisen-Schmidt condensation of the N,N-diacetyl-diketopiperazine 1 with a series of arylaldehydes...
April 25, 2017: Medicinal Chemistry
https://www.readbyqxmd.com/read/28454472/hsp90-inhibitor-sy-016-induces-g2-m-arrest-and-apoptosis-in-paclitaxel-resistant-human-ovarian-cancer-cells
#8
Hyun Gyo Lee, Won Jin Park, So Jin Shin, Sang Hoon Kwon, Soon Do Cha, Young Ho Seo, Ju Hui Jeong, Ji Yoon Lee, Chi Heum Cho
The aim of the present study was to evaluate the in vitro effect of a heat shock protein (Hsp)90 inhibitor, SY-016, on the paclitaxel (PTX)-resistant human ovarian cancer cell line OVCAR-3PTX, and explore its mechanism of apoptosis. In the present study, SY-016 was used in combination with PTX to determine its effect on the cell proliferation and apoptosis of OVCAR-3PTX cells. The drug-resistant tumor cells were established in vitro by stepwise sequential exposure to increasing concentrations of PTX. The cell viability and cell cycle distribution were measured by MTT assay and flow cytometric analysis, respectively...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28413458/mir-9-functions-as-a-tumor-inhibitor-of-cell-proliferation-in-epithelial-ovarian-cancer-through-targeting-the-sdf-1-cxcr4-pathway
#9
Lin He, Li Zhang, Mengfei Wang, Wenrong Wang
The current study aimed to investigate the potential role of miR-9 in the inhibition of ovarian cancer progression through the stromal cell-derived factor-1 (SDF-1)/ C-X-C chemokine receptor type 4 (CXCR4) pathway and to provide a theoretical basis for the diagnosis and treatment of ovarian cancer. Human ovarian cancer OVCAR-3 cells were transfected with miR-9 short hairpin RNA (shRNA). The effect of miR-9 on the mRNA expression levels of CXCR4 were analyzed using reverse transcription-quantitative polymerase chain reaction...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28353633/another-look-at-pyrroloiminoquinone-alkaloids-perspectives-on-their-therapeutic-potential-from-known-structures-and-semisynthetic-analogues
#10
Sheng Lin, Erin P McCauley, Nicholas Lorig-Roach, Karen Tenney, Cassandra N Naphen, Ai-Mei Yang, Tyler A Johnson, Thalia Hernadez, Ramandeep Rattan, Frederick A Valeriote, Phillip Crews
This study began with the goal of identifying constituents from Zyzzya fuliginosa extracts that showed selectivity in our primary cytotoxicity screen against the PANC-1 tumor cell line. During the course of this project, which focused on six Z. fuliginosa samples collected from various regions of the Indo-Pacific, known compounds were obtained consisting of nine makaluvamine and three damirone analogues. Four new acetylated derivatives were also prepared. High-accuracy electrospray ionization mass spectrometry (HAESI-MS) m/z ions produced through MS² runs were obtained and interpreted to provide a rapid way for dereplicating isomers containing a pyrrolo[4,3,2-de]quinoline core...
March 29, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28324785/-ub006-a-new-fatty-acid-synthase-inhibitor-and-cytotoxic-agent-without-anorexic-side-effects
#11
Kamil Makowski, Joan Francesc Mir, Paula Mera, Xavier Ariza, Guillermina Asins, Fausto G Hegardt, Laura Herrero, Jordi García, Dolors Serra
C75 is a synthetic anticancer drug that inhibits fatty acid synthase (FAS) and shows a potent anorexigenic side effect. In order to find new cytotoxic compounds that do not impact food intake, we synthesized a new family of C75 derivatives. The most promising anticancer compound among them was UB006 ((4SR,5SR)-4-(hydroxymethyl)-3-methylene-5-octyldihydrofuran-2(3H)-one). The effects of this compound on cytotoxicity, food intake and body weight were studied in UB006 racemic mixture and in both its enantiomers separately...
May 5, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28148395/tissue-factor-factor-viia-complex-triggers-protease-activated-receptor-2-dependent-growth-factor-release-and-migration-in-ovarian-cancer
#12
Alice Chanakira, Pamela R Westmark, Irene M Ong, John P Sheehan
OBJECTIVE: Enhanced tissue factor (TF) expression in epithelial ovarian cancer (EOC) is associated with aggressive disease. Our objective was to evaluate the role of the TF-factor VIIa-protease-activated receptor-2 (PAR-2) pathway in human EOC. METHODS: TCGA RNAseq data from EOC databases were analyzed for PAR expression. Cell and microparticle (MP) associated TF protein expression (Western blot) and MP-associated coagulant activity were determined in human EOC (SKOV-3, OVCAR-3 and CaOV-3) and control cell lines...
April 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28003845/all-trans-retinoic-acid-and-genistein-induce-cell-apoptosis-in-ovcar-3-cells-by-increasing-the-p14-tumor-suppressor-gene
#13
Mehdi Nikbakht Dastjerdi, Saeed Zamani, Mohammad Mardani, Batool Hashemi Beni
In this study, we evaluated the effects of all-trans retinoic acid (ATRA) alone or in combination with genistein (GEN) in p14 tumor suppressor gene and subsequent apoptosis of human ovarian carcinoma cells (OVCAR-3). The cells were treated with ATRA or GEN at concentrations of 50 and 25 μM respectively, either alone or in combination for 24 and 48 h. The cell viability was evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay. The percentage of cell apoptosis was determined using flow cytometry and p14 gene expression was measured using real time PCR...
December 2016: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27906095/resveratrol-and-acetyl-resveratrol-modulate-activity-of-vegf-and-il-8-in-ovarian-cancer-cell-aggregates-via-attenuation-of-the-nf-%C3%AE%C2%BAb-protein
#14
Alexandria B Tino, Kenny Chitcholtan, Peter H Sykes, Ashley Garrill
BACKGROUND: Key features of advanced ovarian cancer include metastasis via cell clusters in the abdominal cavity and increased chemoresistance. Resveratrol and derivatives of resveratrol have been shown to have antitumour properties. The purpose of this study was to investigate the effect of resveratrol and acetyl-resveratrol on 3D cell aggregates of ovarian cancer, and establish if NF-κB signalling may be a potential target. METHODS: Poly-HEMA coated wells were used to produce 3D aggregates of two ovarian cancer cell lines, SKOV-3 and OVCAR-5...
December 1, 2016: Journal of Ovarian Research
https://www.readbyqxmd.com/read/27862665/role-of-human-epididymis-protein-4-in-chemoresistance-and-prognosis-of-epithelial-ovarian-cancer
#15
Seungho Lee, Seowon Choi, Yookyung Lee, Donghae Chung, Suntaek Hong, Nohhyun Park
AIM: Human epididymis protein 4 (HE4) is a novel biomarker for epithelial ovarian cancer. This study was designed to evaluate the role of HE4 in chemo-response against anti-cancer drugs and prognosis of epithelial ovarian cancer. METHODS: HE4-depleted cells and HE4-overexpressing cells were generated. The effect of HE4 gene silencing and overexpression was examined using a cell viability assay after exposure to chemotherapeutic agents and the signaling pathway. We studied the expression of HE4 in ovarian cancer tissue and the prognostic significance...
January 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/27823852/high-paclitaxel-loaded-and-tumor-cell-targeting-hyaluronan-coated-nanoemulsions
#16
Joo-Eun Kim, Young-Joon Park
The purpose of this study was to develop hyaluronan-coated nanoemulsions (HNEs) with high solubilizing capacity and tumor cell targeting capability for the poorly soluble paclitaxel. The HNEs were composed of dl-a-tocopheryl acetate, soybean oil, polysorbate 80, and ferric chloride and were coated with hyaluronic acid (HA) as a targeting moiety. The nanoemulsions (NEs) and HNEs with or without paclitaxel (PTX) were prepared using high-pressure homogenization with a microfluidizer and were lyophilized with d-mannitol...
February 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27823561/synthesis-antitubercular-and-anticancer-activities-of-p-nitrophenylethylenediamine-derived-schiff-bases
#17
Raquel S Amim, Cláudia Pessoa, Maria C S Lourenço, Marcus V N de Souza, Josane A Lessa
BACKGROUND: Schiff bases have been greatly studied in biological field due to their wide range of pharmacological activities, such as antitubercular and antitumour. In the search of novel antitubercular agents, several compounds containing pharmacophoric group of ethambutol have been synthesized and evaluated against mycobacteria species causing tuberculosis. In this work, we investigate whether ethylenediamine, Schiff base as well as nitro group together could contribute to the formation of novel molecules with dual biological activities: antitubercular and anticancer...
November 4, 2016: Medicinal Chemistry
https://www.readbyqxmd.com/read/27792980/synthesis-and-evaluation-of-novel-hybrids-%C3%AE-carboline-4-thiazolidinones-as-potential-antitumor-and-antiviral-agents
#18
Valéria Aquilino Barbosa, Paula Baréa, Renata Sespede Mazia, Tania Ueda-Nakamura, Willian Ferreira da Costa, Mary Ann Foglio, Ana Lucia T Goes Ruiz, João Ernesto de Carvalho, Débora Barbosa Vendramini-Costa, Celso Vataru Nakamura, Maria Helena Sarragiotto
A series of novel hybrids β-carboline-4-thiazolidinones were synthesized and evaluated for their in vitro antitumor activity against human cancer cell lines and for antiviral activity towards Herpes simplex virus type-1 (HSV-1). From the N'-(2-ylidene-4-thiazolidinone)-β-carboline-3-carbohydrazide series (9-11), compounds 9c and 11d were the most active, showing growth inhibition 50% (GI50) values less than 5 μM for all cell lines tested. Compound 9c, bearing the 4-dimethylaminophenyl group at C-1 of β-carboline was selected for further investigation concerning cell death and cell cycle profile, focusing on the human renal adenocarcinoma cell line 786-0...
November 29, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27698947/synthesis-of-thiazolidine-2-4-dione-derivatives-anticancer-antimicrobial-and-dna-cleavage-studies
#19
S Vijaya Laxmi, P Anil, G Rajitha, Asha Jyothi Rao, Peter A Crooks, B Rajitha
In the search of efficient anticancer agents, here, new 5-(4-alkylbenzyledene)thiazolidine-2,4-dione derivatives (5a-g) have been successfully synthesized and characterized and are evaluated for anticancer and antimicrobial activities using DNA cleavage studies. In vitro studies on anticancer activity of compound 5d (NSC: 768619/1) was done against the full panel of 60 human tumor cell lines. The five-level dose activity results revealed that, the compound 5d was active against all the cell lines, it has shown potential activity against leukemia SR (GI50: 2...
October 2016: Journal of Chemical Biology
https://www.readbyqxmd.com/read/27689466/activation-of-estrogen-receptor-alpha-by-estradiol-and-cisplatin-induces-platinum-resistance-in-ovarian-cancer-cells
#20
Sohei Matsumura, Tsuyoshi Ohta, Keiko Yamanouchi, Zhiyang Liu, Takeshi Sudo, Takanobu Kojimahara, Manabu Seino, Megumi Narumi, Seiji Tsutsumi, Toshifumi Takahashi, Kazuhiro Takahashi, Hirohisa Kurachi, Satoru Nagase
Activation of Estrogen receptor (ER) alpha (α) promotes cell growth and influences the response of cancer cell to chemotherapeutic agents. However, the mechanism by which ERα activation antagonizes cells to chemotherapy-induced cytotoxicity remains unclear. Here, we investigated the effect of cisplatin on ERα activation. In addition, we examined whether down-regulation of ERα modulate cisplatin-mediated cytotoxicity using two human ovarian cancer cells (Caov-3 and Ovcar-3) transduced with ERα short hairpin RNA (shRNA)...
September 30, 2016: Cancer Biology & Therapy
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