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https://www.readbyqxmd.com/read/29161797/electromechanical-morcellators-black-box-warning-tribulations-expectations-and-solutions
#1
Papri Sarkar, Emad Mikhail, Lenox Hoyte, Shayne Plosker, Anthony Imudia
Laparoscopic minimally invasive gynecologic surgery (MIGS) for benign conditions results in decreased morbidity, and faster recovery, compared with laparotomy. Tissue fragmentation by electromechanical morcellation permits the removal of large specimens through small laparoscopic incisions. Since the US Food and Drug Administration (FDA) published its safety warning about the risk of power morcellators spreading cancer during fibroid surgery in 2014, power morcellation has become rare. MIGS for fibroids and other large tissue specimens has declined...
November 21, 2017: Minerva Ginecologica
https://www.readbyqxmd.com/read/29161748/whole-body-cryotherapy-as-a-recovery-technique-after-exercise-a-review-of-the-literature
#2
Catriona Rose, Kate M Edwards, Jason Siegler, Kenneth Graham, Corinne Caillaud
This review aims to evaluate the current body of literature investigating the effect of whole body cryotherapy on recovery after exercise. A systematic search was conducted to investigate the effect of whole body cryotherapy (WBC, exposure to temperatures between -110 to -190°C) on markers of recovery after damaging exercise in healthy, physically active subjects. Of the 16 eligible articles extracted, ten induced muscle damage using controlled exercise in a laboratory setting, while six induced damage during sport-specific training...
November 21, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/29161690/catechins-blunt-the-effects-of-oxldl-and-its-primary-metabolite-phosphatidylcholine-hydroperoxide-on-endothelial-dysfunction-through-inhibition-of-oxidative-stress-and-restoration-of-enos-in-rats
#3
Hao-Hsiang Chang, Chen-Yen Chien, Kuo-Hsin Chen, Shih-Chung Huang, Chiang-Ting Chien
BACKGROUND/AIMS: We explored the effects of catechins (decaffeinated green tea extracts containing (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate and (-)-epigallocatechin gallate) on atherosclerosis risk factors, oxidized low-density lipoprotein (oxLDL) and its primary metabolite, phosphatidylcholine hydroperoxide (PCOOH) induced oxidative injury in cultured endothelial cell line and rats. METHODS: We used endothelial cell line and male Wistar rats to determine the effect of catechins on oxLDL or PCOOH induced oxidative injury including apoptosis, H2O2 level, vascular responses and urinary 8-isoprostane and nitrite/nitrate concentration...
November 22, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29161679/the-changes-of-nutrient-composition-of-piled-laying-hen-manure-and-anaerobic-fermentation-for-recycling-as-a-dietary-ingredient-for-ruminants
#4
Tianlong Han, Liping Wang, Yanming Zhang, Jun Zhang, Dongsheng Han, Ning Lv, Xiaohua Han, Guoqiang Zhao, Min Wang
This study investigated the changes of nutrient compositions of piled laying hen manure, detected the physical and chemical components of laying hen manure fermented by mixed strains, and analysed its application effects on feeding ruminants. The results showed that with increasing of piling time, the contents of crude protein (CP) and ether extract (EE) in laying hen manure were reduced, while the contents of crude ash (CA), calcium (Ca) and phosphorus (P) were increased. Fermentation could effectively decrease pH value, reduce Ca/P ratio, lessen the total bacterial counts, and maintain the organisms contents in laying hen manure...
November 18, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29161675/a-comparison-of-two-digestion-methods-for-assessing-heavy-metals-level-in-urban-soils-influenced-by-mining-and-industrial-activities
#5
Khaled A M Alsaleh, Helmut Meuser, Adel R A Usman, Mohammad I Al-Wabel, Abdullah S Al-Farraj
A comparison between two digestion methods of hot plate Hossner (total-total) and USEPA method 3051 (total-recoverable) was carried out to suggest a proper method for determining nine heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn) content of three urban soils affected by mining (Mahd AD'Dahab) or industrial activities (Riyadh and Jubail) at Saudi Arabia. The results showed no significant differences between two digestion methods for Cd, Cu, Pb and Zn in soils affected by mining and for Cr, Cu, Pb and Zn in soils affected by industrial activities...
November 18, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29161572/new-%C3%AE-galactosidase-producers-with-potential-for-prebiotic-synthesis
#6
Sara C Silvério, Eugénia A Macedo, José A Teixeira, Lígia R Rodrigues
β-Galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of β-galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective β-galactosidase producers under submerged fermentation conditions. The crude β-galactosidases were characterized regarding their optimal pH (3...
November 15, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29161525/sea-cucumber-lipid-soluble-extra-fraction-prevents-ovalbumin-induced-allergic-airway-inflammation
#7
Da-In Lee, Shin Ae Kang, Anisuzzaman Md, U-Cheol Jeong, Feng Jin, Seok-Joong Kang, Jeong-Yeol Lee, Hak Sun Yu
In a previous study, our research group demonstrated that sea cucumber (Apostichopus japonicus) extracts ameliorated allergic airway inflammation through CD4(+)CD25(+)Foxp3(+) T (regulatory T; Treg) cell activation and recruitment to the lung. In this study, we aimed to determine which components of sea cucumber contribute to the amelioration of airway inflammation. We used n-hexane fractionation to separate sea cucumber into three phases (n-hexane, alcohol, and solid) and evaluated the ability of each phase to elevate Il10 expression in splenocytes and ameliorate symptoms in mice with ovalbumin (OVA)/alum-induced asthma...
November 21, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29161511/a-customizable-flow-injection-system-for-automated-high-throughput-and-time-sensitive-ion-mobility-spectrometry-and-mass-spectrometry-measurements
#8
Daniel J Orton, Malak M Tfaily, Ronald J Moore, Brian L LaMarche, Xueyun Zheng, Thomas L Fillmore, Rosalie K Chu, Karl K Weitz, Matthew E Monroe, Ryan T Kelly, Richard D Smith, Erin Shammel Baker
To better understand disease conditions and environmental perturbations, multi-omic studies combining proteomic, lipidomic, and metabolomic analyses are vastly increasing in popularity. In a multi-omic study, a single sample is typically extracted in multiple ways and various analyses are performed using different instruments. Thus, one sample becomes many measurements, making high throughput and reproducible evaluations a necessity. One way to address the numerous samples and varying instrumental conditions is to utilize a flow injection analysis (FIA) system for rapid sample injection...
November 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29161498/selective-isolation-of-myosin-subfragment-1-with-a-dna-polyoxovanadate-bioconjugate
#9
Qing Chen, Xue Hu, Dan-Dan Zhang, Xuwei Chen, Jian-Hua Wang
The bioconjugation of a polyoxometalate (POMs), i.e., dodecavanadate (V12O32), to DNA strands produces a functional labeled DNA primer, V12O32-DNA. The grafting of DNA primer onto streptavidin-coated magnetic nanoparticles (SVM) obtains a novel composite, V12O32-DNA@SVM. The high binding-affinity of V12O32 with the ATP binding site in myosin subfragment-1 (S1) facilitates favorable adsorption of myosin, with an efficiency of 99.4% when processing 0.1 mL myosin solution (100 μg/mL) using 0.1 mg composite. Myosin adsorption fits Langmuir model, corresponding to a theoretical adsorption capacity of 613...
November 21, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29161493/detection-of-leishmania-v-guyanensis-in-rhipicephalus-boophilus-microplus-acari-ixodidae-collected-from-pecari-tajacu
#10
Jesús E Rojas-Jaimes, Germán H Correa-Nuñez, Nyshon Rojas, Omar Cáceres-Rey
INTRODUCTION: Previous studies identified the presence of Leishmania infantum in Rhipicephalus sanguineus and indicated the possibility that it could transmit leishmaniasis to a variety of hosts. OBJECTIVE: To identify parasites of Leishmania (Viannia) spp. in ticks collected from wild animals in an endemic area for leishmaniasis. MATERIALS AND METHODS: We performed 81 individual DNA extractions from ticks collected from three Tapirus terrestris and three Pecari tajacu in Madre de Dios, Perú...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29161491/dengue-virus-detection-in-aedes-aegypti-larvae-and-pupae-collected-in-rural-areas-of-anapoima-cundinamarca-colombia
#11
Myriam Lucía Velandia-Romero, Víctor Alberto Olano, Carolina Coronel-Ruiz, Laura Cabezas, María Angélica Calderón-Peláez, Jaime Eduardo Castellanos, María Inés Matiz
INTRODUCTION: There is a high incidence and prevalence of dengue in the department of Cundinamarca, and recently Aedes aegypti, the main vector of dengue virus (DENV), was detected in some of its rural areas. OBJECTIVE: To evaluate viral transovarial transmission in larvae and pupae collected in rural areas of the municipality of Anapoima, Cundinamarca. MATERIALS AND METHODS: Live larvae and pupae were collected from 53 homes and later they were taken to the laboratory in Anapoima, where they were classified, pooled and frozen...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29161490/-estimation-of-time-detection-limit-for-human-cytochrome-b-in-females-of-lutzomyia-evansi
#12
José Gabriel Vergara, Daniel Verbel-Vergara, Ana Milena Montesino, Alveiro Pérez-Doria, Eduar Elías Bejarano
INTRODUCTION: Molecular biology techniques have allowed a better knowledge of sources of blood meals in vector insects. However, the usefulness of these techniques depends on both the quantity of ingested blood and the digestion process in the insect. OBJECTIVE: To identify the time limit for detection of the human cytochrome b (Cyt b) gene in experimentally fed females of Lutzomyia evansi. MATERIALS AND METHODS: Eight groups of L. evansi females were fed on human blood and sacrificed at intervals of 24 hours post-ingestion...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29161488/identifying-trypanosoma-cruzi-discreet-typing-units-in-triatomines-collected-in-different-natural-regions-of-per%C3%A3%C2%BA
#13
Carlos P Padilla, Uriel Alvarado, Gladis Ventura, Deysi Luna-Caipo, Marcial Suárez, José R Tuñoque, Nancy Ruelas-Llerena, Luis A Fachín, Alina Huiza, Lizandro Gonzáles, Julio César Carranza, Gustavo Adolfo Vallejo, Gustavo Adolfo Vallejo, Abraham G Cáceres, Abraham G Cáceres
INTRODUCTION: Trypanosoma cruzi has been divided by international consensus into six discrete typing units (DTU): TcI, TcII, TcIII, TcIV, TcV y TcVI. The factors determining the dynamics of T. cruzi genotypes vector transmission of Chagas' disease in the different geographical regions of Perú are still unknown. OBJECTIVE: To detect and type T. cruzi DTUs from the faeces of seven species of triatomines (Panstrongylus chinai, P. geniculatus, P. herreri, Rhodnius robustus, R...
March 29, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29161468/the-relationship-between-endogenous-%C3%AE-glucuronidase-activity-and-biologically-active-flavones-aglycone-contents-in-hairy-roots-of-baikal-skullcap
#14
V S Dikaya, A I Solovyeva, R A Sidorov, P A Solovyev, A Yu Stepanova
Here we examine the relationship between contents of principal flavones in hairy roots of Scutellaria baicalensis with the activity of the β-glucuronidase (sGUS) enzyme during a culturing cycle. Using RP-HPLC, we show that the highest contents of aglycones, baicalin and wogonin is observed at the growth days 8, 14 and 71 and reach 45, 41 and 62% (based on the total weight of hairy roots of the Baikal skullcap), correspondingly. Their accumulation is accompanied by increase of the sGUS activity, which we determined fluorometrically...
November 21, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29161465/neuromuscular-electrical-stimulation-for-the-prevention-of-venous-thromboembolism
#15
REVIEW
Shahab Hajibandeh, Shahin Hajibandeh, George A Antoniou, James Rh Scurr, Francesco Torella
BACKGROUND: Venous thromboembolism (VTE) is a serious but preventable cause of morbidity and mortality. Neuromuscular electrical stimulation systems (NMES) for the prevention of VTE may be beneficial for patients in whom pharmacological or standard mechanical prophylaxis methods are contraindicated or are regarded as unsafe or impractical. Although findings of experimental studies suggest that NMES reduce venous stasis, the clinical utility and effectiveness of NMES in VTE prevention remain controversial...
November 21, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29161458/proton-pump-inhibitors-for-functional-dyspepsia
#16
REVIEW
Maria Ines Pinto-Sanchez, Yuhong Yuan, Ahmed Hassan, Premysl Bercik, Paul Moayyedi
BACKGROUND: Functional dyspepsia (FD or non-ulcer dyspepsia) is defined as continuous or frequently recurring epigastric pain or discomfort for which no organic cause can be found. Acid suppressive therapy, including proton pump inhibitors (PPIs), has been proposed as a therapeutic option in FD, but its efficacy remains controversial. While PPIs are generally considered safe and well tolerated, they have been associated with adverse events, especially in the long term. For this reason, decisions on whether to initiate or continue PPI therapy should be made based on an appropriate clinical indication...
November 21, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29161363/improved-survival-in-metastatic-germ-cell-cancer
#17
C D Fankhauser, S Sander, L Roth, J Beyer, T Hermanns
Background: The prognostic score of the International Germ Cell Cancer Collaborative Group (IGCCCG) in metastatic germ-cell cancers (mGCC) relies on treatments delivered before 1990. It is unclear, if this score is still relevant to contemporary cohorts of patients who receive modern-type chemotherapy and supportive care. Patients and Methods: All patients who underwent cisplatin/etoposide based first-line chemotherapy for mGCC at the University Hospital Zurich (USZ) between 1991 and 2016 were identified retrospectively...
November 17, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/29161330/fasciola-spp-mapping-of-the-mf6-epitope-and-antigenic-analysis-of-the-mf6p-hdm-family-of-heme-binding-proteins
#18
Victoria Martínez-Sernández, María J Perteguer, Mercedes Mezo, Marta González-Warleta, Teresa Gárate, M Adela Valero, Florencio M Ubeira
MF6p/FhHDM-1 is a small cationic heme-binding protein which is recognized by the monoclonal antibody (mAb) MF6, and abundantly present in parenchymal cells and secreted antigens of Fasciola hepatica. Orthologs of this protein (MF6p/HDMs) also exist in other causal agents of important foodborne trematodiasis, such as Clonorchis sinensis, Opisthorchis viverrini and Paragonimus westermani. Considering that MF6p/FhHDM-1 is relevant for heme homeostasis in Fasciola and was reported to have immunomodulatory properties, this protein is expected to be a useful target for vaccination...
2017: PloS One
https://www.readbyqxmd.com/read/29161291/declust-a-statistical-approach-for-obtaining-differential-expression-profiles-of-multiple-conditions
#19
Yoshimasa Aoto, Tsuyoshi Hachiya, Kazuhiro Okumura, Sumitaka Hase, Kengo Sato, Yuichi Wakabayashi, Yasubumi Sakakibara
High-throughput RNA sequencing technology is widely used to comprehensively detect and quantify cellular gene expression. Thus, numerous analytical methods have been proposed for identifying differentially expressed genes (DEGs) between paired samples such as tumor and control specimens, but few studies have reported methods for analyzing differential expression under multiple conditions. We propose a novel method, DEclust, for differential expression analysis among more than two matched samples from distinct tissues or conditions...
2017: PloS One
https://www.readbyqxmd.com/read/29161268/nucleic-acid-purification-from-plants-animals-and-microbes-in-under-30-seconds
#20
Yiping Zou, Michael Glenn Mason, Yuling Wang, Eugene Wee, Conny Turni, Patrick J Blackall, Matt Trau, Jose Ramon Botella
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed...
November 2017: PLoS Biology
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