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https://www.readbyqxmd.com/read/28813499/staphylococcus-aureus-biofilm-elicits-the-expansion-activation-and-polarization-of-myeloid-derived-suppressor-cells-in-vivo-and-in-vitro
#1
Kuo-Ti Peng, Ching-Chuan Hsieh, Tsung-Yu Huang, Pei-Chun Chen, Hsin-Nung Shih, Mel S Lee, Pey-Jium Chang
Staphylococcus aureus (S. aureus) is one of the most common causes of biofilm infections in periprosthetic joint infections (PJIs). Accumulating evidence has shown that the immunosuppressive environment established by S. aureus biofilm infection in PJIs involves the presence of myeloid-derived suppressor cells (MDSCs) and M2-macrophages. Due to the diversity of MDSCs, little is known about whether S. aureus biofilm preferentially expands specific MDSC subsets or whether MDSCs can further differentiate into M2-macrophages during S...
2017: PloS One
https://www.readbyqxmd.com/read/28657582/biocompatibility-of-hbv-liposome-encapsulated-hemoglobin-molecules-liposome-effects-on-immune-function
#2
REVIEW
Hiroshi Azuma, Mitsuhiro Fujihara, Hiromi Sakai
Hemoglobin vesicles (HbVs) are oxygen carriers consisting of Hb molecules and liposome in which human hemoglobin (Hb) molecules are encapsulated. Investigations of HbV biocompatibility have shown that HbVs have no significant effect on either the quality or quantity of blood components such as RBC, WBC, platelets, complements, or coagulation factors, reflecting its excellent biocompatibility. However, their effects on the immune system remain to be evaluated. HbVs might affect the function of macrophages because they accumulate in the reticuloendothelial system...
June 28, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28539643/myostatin-a-profibrotic-factor-and-the-main-inhibitor-of-striated-muscle-mass-is-present-in-the-penile-and-vascular-smooth-muscle
#3
I Kovanecz, M Masouminia, R Gelfand, D Vernet, J Rajfer, N F Gonzalez-Cadavid
Myostatin is present in striated myofibers but, except for myometrial cells, has not been reported within smooth muscle cells (SMC). We investigated in the rat whether myostatin is present in SMC within the penis and the vascular wall and, if so, whether it is transcriptionally expressed and associated with the loss of corporal SMC occurring in certain forms of erectile dysfunction (ED). Myostatin protein was detected by immunohistochemistry/fluorescence and western blots in the perineal striated muscles, and also in the SMC of the penile corpora, arteries and veins, and aorta...
September 2017: International Journal of Impotence Research
https://www.readbyqxmd.com/read/28378599/liposomal-microparticle-injection-can-induce-myeloid-derived-suppressor-cells-mdsc-like-cells-in-vivo
#4
Hiroshi Azuma, Yoichiro Yoshida, Hironori Takahashi, Emi Ishibazawa, Hiroya Kobayashi, Hiromi Sakai, Daisuke Takahashi, Mitsuhiro Fujihara
CONTEXT: Myeloid-derived suppressor cells (MDSCs) are a subset of immature myeloid cells that function as immunosuppressive cells in various pathological conditions. Membrane-derived microvesicles are thought to be involved in MDSC induction. Earlier reports have described that injection of considerable amount of liposome into rat can suppress Con A-induced splenic T-cell proliferation. Liposome-internalized cells expressing CD11b/c suppress T-cell proliferation. Nitric oxide (NO) appears to be involved in the suppression...
June 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/28217409/muscle-derived-stem-cells-stimulate-muscle-myofiber-repair-and-counteract-fat-infiltration-in-a-diabetic-mouse-model-of-critical-limb-ischemia
#5
J Tsao, I Kovanecz, N Awadalla, R Gelfand, I Sinha-Hikim, R A White, N F Gonzalez-Cadavid
BACKGROUND: Critical Limb Ischemia (CLI) affects patients with Type 2 Diabetes (T2D) and obesity, with high risk of amputation and post-surgical mortality, and no effective medical treatment. Stem cell therapy, mainly with bone marrow mesenchymal, adipose derived, endothelial, hematopoietic, and umbilical cord stem cells, is promising in CLI mouse and rat models and is in clinical trials. Their general focus is on angiogenic repair, with no reports on the alleviation of necrosis, lipofibrosis, and myofiber regeneration in the ischemic muscle, or the use of Muscle Derived Stem Cells (MDSC) alone or in combination with pharmacological adjuvants, in the context of CLI in T2D...
December 2016: Journal of Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27810640/skeletal-muscle-derived-stem-cells-microintegrated-into-a-biodegradable-elastomer-for-reconstruction-of-the-abdominal-wall
#6
Keisuke Takanari, Ryotaro Hashizume, Yi Hong, Nicholas J Amoroso, Tomo Yoshizumi, Burhan Gharaibeh, Osamu Yoshida, Kazuhiro Nonaka, Hideyoshi Sato, Johnny Huard, William R Wagner
A variety of techniques have been applied to generate tissue engineered constructs, where cells are combined with degradable scaffolds followed by a period of in vitro culture or direct implantation. In the current study, a cellularized scaffold was generated by concurrent deposition of electrospun biodegradable elastomer (poly(ester urethane)urea, PEUU) and electrosprayed culture medium + skeletal muscle-derived stem cells (MDSCs) or electrosprayed culture medium alone as a control. MDSCs were obtained from green fluorescent protein (GFP) transgenic rats...
January 2017: Biomaterials
https://www.readbyqxmd.com/read/27549627/mesothelioma-response-to-carbon-nanotubes-is-associated-with-an-early-and-selective-accumulation-of-immunosuppressive-monocytic-cells
#7
François Huaux, Virginie d'Ursel de Bousies, Marie-Astrid Parent, Micaela Orsi, Francine Uwambayinema, Raynal Devosse, Saloua Ibouraadaten, Yousof Yakoub, Nadtha Panin, Mihaly Palmai-Pallag, Pierre van der Bruggen, Christian Bailly, Riccardo Marega, Etienne Marbaix, Dominique Lison
BACKGROUND: The asbestos-like toxicity of some engineered carbon nanotubes (CNT), notably their capacity to induce mesothelioma, is a serious cause of concern for public health. Here we show that carcinogenic CNT induce an early and sustained immunosuppressive response characterized by the accumulation of monocytic Myeloid Derived Suppressor Cells (M-MDSC) that counteract effective immune surveillance of tumor cells. METHODS: Wistar rats and C57BL/6 mice were intraperitoneally injected with carcinogenic multi-walled Mitsui-7 CNT (CNT-7) or crocidolite asbestos...
August 23, 2016: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/27114192/implanted-muscle-derived-stem-cells-ameliorate-erectile-dysfunction-in-a-rat-model-of-type-2-diabetes-but-their-repair-capacity-is-impaired-by-their-prior-exposure-to-the-diabetic-milieu
#8
Istvan Kovanecz, Dolores Vernet, Maryam Masouminia, Robert Gelfand, Leila Loni, James Aboagye, James Tsao, Jacob Rajfer, Nestor F Gonzalez-Cadavid
INTRODUCTION: Muscle-derived stem cells (MDSCs) and other SCs implanted into the penile corpora cavernosa ameliorate erectile dysfunction in type 1 diabetic rat models by replenishing lost corporal smooth muscle cells (SMCs) and decreasing fibrosis. However, there are no conclusive data from models of type 2 diabetes (T2D) and obesity. AIM: To determine whether MDSCs from obese Zucker (OZ) rats with T2D at an early stage of diabetes (early diabetic SCs isolated and cultured in low-glucose medium [ED-SCs]) counteract corporal veno-occlusive dysfunction and corporal SMC loss or lipo-fibrosis when implanted in OZ rats at a late stage of diabetes and whether MDSCs from these OZ rats with late diabetes (late diabetic SCs isolated and cultured in high-glucose medium [LD-SC]) differ from ED-SCs in gene transcriptional phenotype and repair capacity...
May 2016: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/26816756/microsurgical-denervation-of-the-rat-spermatic-cord-and-its-connection-to-the-diagnosis-and-treatment-of-chronic-orchialgia-a-bench-to-bedside-experience
#9
REVIEW
Asha E Jamzadeh, Melissa A Laudano, E Charles Osterberg, Marc Goldstein, Philip S Li
Chronic orchialgia is a common urologic problem, however, determination of the etiology is often difficult and the pathophysiology is poorly understood. As a result, there is no clear algorithm for surgical treatment for men who have failed conservative medical treatment. This review aims to describe microsurgical denervation of the rat spermatic cord (SC) and summarize several surgical techniques that have been described in the literature ranging from orchiectomy to epididymectomy to vasectomy reversal for post-vasectomy orchialgia...
March 2014: Translational Andrology and Urology
https://www.readbyqxmd.com/read/26471056/development-of-hot-melt-co-formulated-antimalarial-solid-dispersion-system-in-fixed-dose-form-arlumelt-evaluating-amorphous-state-and-in-vivo-performance
#10
COMPARATIVE STUDY
Ritesh Fule, Dinesh Dhamecha, Mohammed Maniruzzaman, Anubha Khale, Purnima Amin
The aim of this study was to investigate the industrial feasibility of developing a co-formulated solid dispersion (SD) containing two antimalarial drugs artemether (ARTM) and lumefantrine (LUMF). Soluplus(®) (polyethyleneglycol-polyvinyl caprolactam-polyvinyl acetate grafted copolymer) was used as primary carrier matrices via hot-melt extrusion processing to improve solubility profile and the oral bioavailability of the combination. Based on the preliminary screening, the optimized quantities of PEG 400, Lutrol F127 and Lutrol F68 were incorporated as surfactant with soluplus in different ratios to improve extrudability, increase wettability and the melt viscosity of the HME process...
December 30, 2015: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/26405600/cancer-associated-fibroblasts-have-phenotypic-and-functional-characteristics-similar-to-the-fibrocytes-that-represent-a-novel-mdsc-subset
#11
Gurcan Gunaydin, S Altug Kesikli, Dicle Guc
Circulating fibrocytes were reported to represent a novel myeloid-derived suppressor cell (MDSC) subset and they were also proposed to be involved in the tumor immune escape. This novel fibrocyte subset had a surface phenotype resembling non-monocytic MDSCs (CD14(-)CD11c(hi)CD123(-)) and exhibited immunomodulatory roles. Most effector functions of fibrocytes (circulating fibroblast-progenitors) are accomplished as tissue fibroblasts, likewise in the tumor microenvironment. Therefore, fibroblasts at tumor tissues should be evaluated whether they display similar molecular/gene expression patterns and functional roles to the blood-borne fibrocytes...
September 2015: Oncoimmunology
https://www.readbyqxmd.com/read/25732602/granulocytic-subset-of-myeloid-derived-suppressor-cells-in-rats-with-mammary-carcinoma
#12
Yusuf Dolen, Gurcan Gunaydin, Gunes Esendagli, Dicle Guc
Limited knowledge is available on myeloid derived suppressor cells (MDSCs) of rat origin. We examined the myeloid cells from peripheral blood, bone marrow and spleens of healthy and mammary tumor bearing rats employing a novel immunophenotyping strategy with CD172a, HIS48, and Rp-1 antibodies. We addressed rat granulocytes by Rp-1 positivity and used HIS48 in discrimination of two mononuclear cell subsets. An expansion of granulocyte numbers was detected in peripheral blood and spleens of mammary tumor-bearing animals...
May 2015: Cellular Immunology
https://www.readbyqxmd.com/read/25678492/optical-in-vivo-imaging-of-the-alarmin-s100a9-in-tumor-lesions-allows-for-estimation-of-the-individual-malignant-potential-by-evaluation-of-tumor-host-cell-interaction
#13
Anne Becker, Nils Große Hokamp, Stefanie Zenker, Fabian Flores-Borja, Katarzyna Barzcyk, Georg Varga, Johannes Roth, Christiane Geyer, Walter Heindel, Christoph Bremer, Thomas Vogl, Michel Eisenblaetter
UNLABELLED: Tumors recruit and reprogram immune cells to support tumor development and spread, the most prominent among them being of monocytic origin such as tumor-associated macrophages (TAM) or myeloid-derived suppressor cells (MDSC). The alarmin S100A8/A9 has been implicated in the induction of TAM and MDSC. We assessed S100A9 as a molecular imaging marker for the activity of tumor-associated immune cells in a syngeneic murine breast cancer model. S100A9 could serve as a surrogate marker for tumor immune crosstalk as a function of malignancy, providing a tool with the potential for both basic research in tumor immunology and clinical stratification of patients...
March 2015: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/24643056/estradiol-modulates-the-expression-pattern-of-myosin-heavy-chain-subtypes-via-an-er%C3%AE-mediated-pathway-in-muscle-derived-tissues-and-satellite-cells
#14
Tao Guo, Wenjia Liu, Anna Konermann, Zexu Gu, Meng Cao, Yin Ding
BACKGROUND: Muscle-derived satellite cells (MDSCs) express MHC molecules intimately related to muscle function, which is supposed to be affected by local estrogen (E2) levels. However, cellular targets and molecular mechanisms involved are poorly understood. METHODS: Genioglossus (GG) muscle tissues and MDSCs were derived from SHAM, ovariectomized or ovariectomized and 17 β-estradiol injected rats (n=10/group). ERα, ERβ, MHC expression and underlying regulatory mechanisms were investigated by RT-PCR, western blot and immunohistochemistry, inter alia upon selective antagonist exposure and Si-RNA transfection...
2014: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/24053156/microsurgical-denervation-of-rat-spermatic-cord-safety-and-efficacy-data
#15
Melissa A Laudano, E Charles Osterberg, Seema Sheth, Ranjith Ramasamy, Joshua Sterling, Sushmita Mukherjee, Brian D Robinson, Sijo Parekattil, Marc Goldstein, Peter N Schlegel, Philip S Li
OBJECTIVE: To describe a microsurgical technique for denervation of the spermatic cord and use of multiphoton microscopy (MPM) laser to identify and ablate residual nerves after microsurgical denervation. To evaluate structural and functional changes in the rat testis and vas deferens after denervation. MATERIALS AND METHODS: Nine Sprague-Dawley rats were divided into three experimental groups: sham, microsurgical denervation of the spermatic cord (MDSC), and MDSC immediately followed by laser ablation with MPM...
May 2014: BJU International
https://www.readbyqxmd.com/read/23308231/all-trans-retinoic-acid-in-combination-with-primaquine-clears-pneumocystis-infection
#16
Guang-Sheng Lei, Chen Zhang, Shoujin Shao, Hsin-Wei Jung, Pamela J Durant, Chao-Hung Lee
Pneumocystis pneumonia (PcP) develops in immunocompromised patients. Alveolar macrophages play a key role in the recognition, phagocytosis, and degradation of Pneumocystis, but their number is decreased in PcP. Our study of various inflammatory components during PcP found that myeloid-derived suppressor cells (MDSCs) accumulate in the lungs of mice and rats with Pneumocystis pneumonia (PcP). We hypothesized that treatment with all-trans retinoic acid (ATRA), a metabolite of vitamin A, may effectively control Pneumocystis (Pc) infection by inducing MDSCs to differentiate to AMs...
2013: PloS One
https://www.readbyqxmd.com/read/23252661/liver-exhibits-thermal-variations-according-to-the-stage-of-fibrosis-progression-a-novel-use-of-modulated-differential-scanning-calorimetry-for-research-in-hepatology
#17
Galileo Escobedo, José Luis Arjona-Román, Rosalía Meléndez-Pérez, Karina Suárez-Álvarez, Carolina Guzmán, Jesús Aguirre-García, Gabriela Gutiérrez-Reyes, Oscar Vivas, Gustavo Varela-Fascinetto, Adela Rodríguez-Romero, Guillermo Robles-Díaz, David Kershenobich
AIM: Liver fibrosis results in a disproportion of the hepatic composition and architecture, characterized by a progressive accumulation of fibrillar proteins at the liver parenchyma. Modulated-differential scanning calorimetry (mDSC) is an experimental methodology able to determine the specific thermal signature from any biological substance, based on the variation in heat flow and heat capacity. As these physicochemical properties are directly influenced by compositional and structural changes, we decided to study the thermal behavior of the liver during fibrosis using mDSC...
July 2013: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/23251266/recovery-from-rat-sciatic-nerve-injury-in-vivo-through-the-use-of-differentiated-mdscs-in-vitro
#18
Xiangyi Zeng, Li Zhang, Liang Sun, Dai Zhang, Hengwu Zhao, Jun Jia, Wei Wang
In this study, muscle-derived stem cells (MDSCs) whose differentiation into neuron-like cells was induced by ciliary neurotrophic factor (CNTF) and Salvia (Salvia miltiorrhiza) in vitro were used to repair rat sciatic nerve injuries in vivo, in order to investigate their multifunctional characteristics as pluripotent stem cells. The sciatic nerve in the right side of the lower limb was exposed under the anesthetized condition of 10% chloral hydrate (0.3 ml/100 g) injection into the abdominal cavity. The tissue which was 0...
January 2013: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/23082782/muscle-derived-stem-cells-promote-angiogenesis-and-attenuate-intimal-hyperplasia-in-different-murine-vascular-disease-models
#19
Hyung Sub Park, Soli Hahn, Geum Hee Choi, Young Sun Yoo, Ji Youl Lee, Taeseung Lee
Muscle-derived stem cells (MDSCs) are known to promote angiogenesis, but have never been studied in vascular diseases. We differentiated MDSCs into endothelial lineage cells in vitro by stimulation with shear stress and vascular endothelial growth factor. Such differentiated MDSCs (diff-MDSC) showed strong angiogenic potential in vitro. When tested in ischemic hindlimbs of mice, diff-MDSCs increased perfusion and decreased necrosis of the ischemic limbs, by promoting new vessel formation and by upregulating genes involved in endothelial expression...
March 15, 2013: Stem Cells and Development
https://www.readbyqxmd.com/read/23041435/the-effect-of-platelet-rich-plasma-on-the-regenerative-therapy-of-muscle-derived-stem-cells-for-articular-cartilage-repair
#20
Y Mifune, T Matsumoto, K Takayama, S Ota, H Li, L B Meszaros, A Usas, K Nagamune, B Gharaibeh, F H Fu, J Huard
OBJECTIVE: Platelet-rich plasma (PRP) is reported to promote collagen synthesis and cell proliferation as well as enhance cartilage repair. Our previous study revealed that the intracapsular injection of muscle derived stem cells (MDSCs) expressing bone morphogenetic protein 4 (BMP-4) combined with soluble Flt-1 (sFlt1) was effective for repairing articular cartilage (AC) after osteoarthritis (OA) induction. The current study was undertaken to investigate whether PRP could further enhance the therapeutic effect of MDSC therapy for the OA treatment...
January 2013: Osteoarthritis and Cartilage
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