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https://www.readbyqxmd.com/read/27922671/differential-detection-of-cytoplasmic-wilms-tumor-1-expression-by-immunohistochemistry-western-blotting-and-mrna-quantification
#1
Takehiro Maki, Hiroaki Ikeda, Aki Kuroda, Noriaki Kyogoku, Yoshiyuki Yamamura, Yukiko Tabata, Takehiro Abiko, Takahiro Tsuchikawa, Yasuhiro Hida, Toshiaki Shichinohe, Eiichi Tanaka, Kichizo Kaga, Kanako Hatanaka, Yoshihiro Matsuno, Naoko Imai, Satoshi Hirano
Wilms tumor 1 (WT1) is considered to be a promising target of cancer treatment because it has been reported to be frequently expressed at high levels in various malignancies. Although WT1-targeted cancer treatment has been initiated, conclusive detection methods for WT1 are not established. The present study aimed to consolidate immunohistochemistry for WT1 with statistical basis. Transfected cells with forced WT1 expression yielded specific western blot bands and nuclear immunostaining; cytoplasmic immunostaining was not specifically recognized...
December 2, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27919249/-the-microbiome-and-the-pathophysiology-of-asthma
#2
REVIEW
Ashley Sullivan, Eoin Hunt, John MacSharry, Desmond M Murphy
Asthma is a chronic respiratory disease whose prevalence is increasing in the western world. Recently research has begun to focus on the role the microbiome plays in asthma pathogenesis in the hope of further understanding this respiratory disorder. Considered sterile until recently, the lungs have revealed themselves to contain a unique microbiota. A shift towards molecular methods for the quantification and sequencing of microbial DNA has revealed that the airways harbour a unique microbiota with apparent, reproducible differences present between healthy and diseased lungs...
December 5, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27913729/qsea-modelling-of-genome-wide-dna-methylation-from-sequencing-enrichment-experiments
#3
Matthias Lienhard, Sabrina Grasse, Jana Rolff, Steffen Frese, Uwe Schirmer, Michael Becker, Stefan Börno, Bernd Timmermann, Lukas Chavez, Holger Sültmann, Gunda Leschber, Iduna Fichtner, Michal R Schweiger, Ralf Herwig
Genome-wide enrichment of methylated DNA followed by sequencing (MeDIP-seq) offers a reasonable compromise between experimental costs and genomic coverage. However, the computational analysis of these experiments is complex, and quantification of the enrichment signals in terms of absolute levels of methylation requires specific transformation. In this work, we present QSEA, Quantitative Sequence Enrichment Analysis, a comprehensive workflow for the modelling and subsequent quantification of MeDIP-seq data...
December 1, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27906622/sensitive-liquid-chromatography-mass-spectrometry-lc-ms-assay-reveals-novel-insights-on-dna-methylation-and-incorporation-of-gemcitabine-its-metabolite-difluorodeoxyuridine-deoxyuridine-and-rx-3117-into-dna
#4
Richard J Honeywell, Dzjemma Sarkisjan, Ietje Kathmann, Michael H Kristensen, Godefridus J Peters
Antimetabolites are incorporated into DNA and RNA, affecting their function. Liquid-chromatography-mass-spectrometry (LC-MS-MS) permits the sensitive, selective analysis of normal nucleosides. The method was adapted to measure the incorporation of deoxyuridine, gemcitabine (difluorodeoxycytidine), its metabolite difluorodeoxyuridine (dFdU), and the novel compound fluorocyclopentenylcytosine (RX3117). DNA was degraded to its deoxynucleotides for quantification by LC-MS-MS, gradient chromatography on a Phenomenex prodigy-3-ODS with positive ionization...
December 2016: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/27906445/the-expression-research-of-mir-210-in-the-elderly-patients-with-copd-combined-with-ischemic-stroke
#5
L-Q Wang, C-L Wang, L-N Xu, D-F Hua
OBJECTIVE: We conduct this study to investigate the expression level of miR-210 in elderly patients with COPD combined with ischemic stroke and analyzed its application value as a sensitive diagnostic indicator. PATIENTS AND METHODS: 50 cases of elderly patients with COPD combined with ischemic stroke were selected as group A, 50 cases of elderly patients with COPD were selected as group B, 50 cases of elderly patients with ischemic stroke were selected as group C, and 50 cases of healthy volunteers as group D...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27902909/marek-s-disease-in-backyard-chickens-a-study-of-pathologic-findings-and-viral-loads-in-tumorous-and-nontumorous-birds
#6
Aslı Mete, Radhika Gharpure, Maurice E Pitesky, Dan Famini, Karen Sverlow, John Dunn
Marek's disease (MD) is a major cause of mortality in backyard chickens. The diagnosis of MD is complex, however, and knowledge of Marek's disease virus (MDV) in spontaneous field cases such as in backyard chickens is largely unknown. In this study, 40 backyard chickens with a presumptive MD diagnosis based on histologic lymphoid infiltrations in peripheral nerves with and without lymphomas were investigated. Twenty-eight of the birds were submitted to the diagnostic laboratory for disease explorations, and 12 chickens were from a flock in which some members demonstrated anisocoria and pupil irregularities compatible with ocular MD...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27902611/severity-of-pulmonary-emphysema-and-lung-cancer-analysis-using-quantitative-lobar-emphysema-scoring
#7
Kyungsoo Bae, Kyung Nyeo Jeon, Seung Jun Lee, Ho Cheol Kim, Ji Young Ha, Sung Eun Park, Hye Jin Baek, Bo Hwa Choi, Soo Buem Cho, Jin Il Moon
The aim of this study was to determine the relationship between lobar severity of emphysema and lung cancer using automated lobe segmentation and emphysema quantification methods.This study included 78 patients (74 males and 4 females; mean age of 72 years) with the following conditions: pathologically proven lung cancer, available chest computed tomographic (CT) scans for lobe segmentation, and quantitative scoring of emphysema. The relationship between emphysema and lung cancer was analyzed using quantitative emphysema scoring of each pulmonary lobe...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27859436/tissue-distribution-of-capilliposide-b-capilliposide-c-and-their-bioactive-metabolite-in-mice-using-liquid-chromatography-tandem-mass-spectrometry
#8
Zhongzhe Cheng, Xing Zhou, Bingying Hu, Wenyi Li, Guiying Chen, Yang Zhang, Jingkui Tian, Lin Zhang, Ming Li, Hongliang Jiang
Lysimachia capillipes Hemsl (Primulaceae), a folk medicinal plant in China, showed significant anti-tumor activities in vivo and vitro. Capilliposide B (LC-B) and Capilliposide C (LC-C) were the main bioactive components in this plant. To explore their tissue distribution, a reliable bioanalytical method for the quantification of LC-B, LC-C, and their bioactive metabolite, Capilliposide A (LC-A) in mice tissues was developed and validated. In this study, the tissue distribution profiles of the three compounds were examined after intravenous administration of pure LC-B and oral administration of total saponins of L...
November 14, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27855182/a-non-human-primate-model-of-severe-pneumococcal-pneumonia
#9
Luis F Reyes, Marcos I Restrepo, Cecilia A Hinojosa, Nilam J Soni, Anukul T Shenoy, Ryan P Gilley, Norberto Gonzalez-Juarbe, Julio R Noda, Vicki T Winter, Melissa A de la Garza, Robert E Shade, Jacqueline J Coalson, Luis D Giavedoni, Antonio Anzueto, Carlos J Orihuela
RATIONALE: Streptococcus pneumoniae is the leading cause of community-acquired pneumonia and infectious death in adults worldwide. A non-human primate model is needed to study the molecular mechanisms that underlie the development of severe pneumonia, identify diagnostic tools, explore potential therapeutic targets, and test clinical interventions during pneumococcal pneumonia. OBJECTIVE: To develop a non-human primate model of pneumococcal pneumonia. METHODS: Seven adult baboons (Papio cynocephalus) were surgically tethered to a continuous monitoring system that recorded heart rate, temperature, and electrocardiography...
2016: PloS One
https://www.readbyqxmd.com/read/27847999/impact-of-iterative-reconstructions-on-objective-and-subjective-emphysema-assessment-with-computed-tomography-a-prospective-study
#10
Steve P Martin, Joanna Gariani, Anne-Lise Hachulla, Diomidis Botsikas, Dan Adler, Wolfram Karenovics, Christoph D Becker, Xavier Montet
OBJECTIVES: To prospectively evaluate the impact of iterative reconstruction (IR) algorithms on pulmonary emphysema assessment as compared to filtered back projection (FBP). METHODS: One hundred ten unenhanced chest CT examinations were obtained on two different scanners. Image reconstructions from a single acquisition were done with different levels of IR and compared with FBP on the basis of the emphysema index (EI), lung volume and voxel densities. Objective emphysema assessment was performed with 3D software provided by each manufacturer...
November 15, 2016: European Radiology
https://www.readbyqxmd.com/read/27842667/fdg-pet-ct-in-the-prediction-of-pulmonary-function-improvement-in-nonspecific-interstitial-pneumonia-a-pilot-study
#11
V Jacquelin, A Mekinian, P Y Brillet, H Nunes, O Fain, D Valeyre, M Soussan
PURPOSE: Our study aimed to analyse the characteristics of nonspecific interstitial pneumonia (NSIP) using FDG-PET/CT (PET) and to evaluate its ability to predict the therapeutic response. PROCEDURES: Eighteen NSIP patients were included. Maximum standardized uptake value (SUVmax), FDG uptake extent (in percentage of lung volume), high resolution CT scan (HRCT) elementary lesions, and HRCT fibrosis score were recorded. The predictive value of the parameters for lung function improvement was evaluated using logistic regression and Receiver Operating Characteristic (ROC) curve analysis (n=13/18)...
December 2016: European Journal of Radiology
https://www.readbyqxmd.com/read/27834143/distribution-of-synthetic-cannabinoids-jwh-210-rcs-4-and-%C3%A2-9-tetrahydrocannabinol-after-intravenous-administration-to-pigs
#12
Nadine Schaefer, Mattias Kettner, Matthias W Laschke, Julia Schlote, Andreas H Ewald, Michael D Menger, Hans H Maurer, Peter H Schmidt
Synthetic cannabinoids (SCs) have become an increasing issue in forensic toxicology. Controlled human studies evaluating pharmacokinetic data of SCs are lacking and only few animal studies have been published. Thus, an interpretation of analytical results found in intoxicated or poisoned individuals is difficult. Therefore, the distribution of two selected SCs, namely 4-ethylnaphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indol-3-yl)methanone (RCS-4) as well as ∆9-tetrahydrocannabinol (THC) as reference were examined in pigs...
November 11, 2016: Current Neuropharmacology
https://www.readbyqxmd.com/read/27830712/a-dynamic-in-vivo-like-organotypic-blood-brain-barrier-model-to-probe-metastatic-brain-tumors
#13
Hui Xu, Zhongyu Li, Yue Yu, Saman Sizdahkhani, Winson S Ho, Fangchao Yin, Li Wang, Guoli Zhu, Min Zhang, Lei Jiang, Zhengping Zhuang, Jianhua Qin
The blood-brain barrier (BBB) restricts the uptake of many neuro-therapeutic molecules, presenting a formidable hurdle to drug development in brain diseases. We proposed a new and dynamic in vivo-like three-dimensional microfluidic system that replicates the key structural, functional and mechanical properties of the blood-brain barrier in vivo. Multiple factors in this system work synergistically to accentuate BBB-specific attributes-permitting the analysis of complex organ-level responses in both normal and pathological microenvironments in brain tumors...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27825367/involvement-of-the-different-lung-compartments-in-the-pathogenesis-of-ph1n1-influenza-virus-infection-in-ferrets
#14
Beatriz Vidaña, Jorge Martínez, Jaime Martorell, María Montoya, Lorena Córdoba, Mónica Pérez, Natàlia Majó
Severe cases after pH1N1 infection are consequence of interstitial pneumonia triggered by alveolar viral replication and an exacerbated host immune response, characterized by the up-regulation of pro-inflammatory cytokines and the influx of inflammatory leukocytes to the lungs. Different lung cell populations have been suggested as culprits in the unregulated innate immune responses observed in these cases. This study aims to clarify this question by studying the different induction of innate immune molecules by the distinct lung anatomic compartments (vascular, alveolar and bronchiolar) of ferrets intratracheally infected with a human pH1N1 viral isolate, by means of laser microdissection techniques...
November 8, 2016: Veterinary Research
https://www.readbyqxmd.com/read/27820928/pulmonary-perfusion-changes-as-assessed-by-contrast-enhanced-dual-energy-computed-tomography-after-endoscopic-lung-volume-reduction-by-coils
#15
Frédéric Lador, Anne-Lise Hachulla, Olivia Hohn, Jérôme Plojoux, Maxime Ronot, Xavier Montet, Paola M Soccal
BACKGROUND: Endoscopic lung volume reduction by coils (LVRC) is a recent treatment approach for severe emphysema. Furthermore, dual-energy computed tomography (DECT) now offers a combined assessment of lung morphology and pulmonary perfusion. OBJECTIVES: The aim of our study was to assess the impact of LVRC on pulmonary perfusion with DECT. METHODS: Seventeen patients (64.8 ± 6.7 years) underwent LVRC. DECT was performed prior to and after LVRC...
2016: Respiration; International Review of Thoracic Diseases
https://www.readbyqxmd.com/read/27812791/generating-harmonized-suv-within-the-eanm-earl-accreditation-program-software-approach-versus-earl-compliant-reconstruction
#16
Charline Lasnon, Thibault Salomon, Cédric Desmonts, Pascal Dô, Youssef Oulkhouir, Jeannick Madelaine, Nicolas Aide
BACKGROUND: Evolutions in hardware and software PET technology, such as point spread function (PSF) reconstruction, have been shown to improve diagnostic performance, but can also lead to important device-dependent and reconstruction-dependent variations in standardized uptake values (SUVs). This may preclude the multicentre use of SUVs as a prognostic or diagnostic tool or as a biomarker of the early response to antineoplastic treatments. This study compared two SUV harmonization strategies using a newer reconstruction algorithm that improves lesion detection while maintaining comparability with older systems: (1) the use of a second reconstruction compliant with harmonization standards and (2) the use of a proprietary software tool (EQ...
November 3, 2016: Annals of Nuclear Medicine
https://www.readbyqxmd.com/read/27809405/pulmonary-perfusion-quantification-with-flow-sensitive-inversion-recovery-fair-ute-mri-in-small-animal-imaging
#17
Marta Tibiletti, Andrea Bianchi, Detlef Stiller, Volker Rasche
Blood perfusion in lung parenchyma is an important property for assessing lung function. In small animals, its quantitation is limited even with radioactive isotopes or dynamic contrast-enhanced MRI techniques. In this study, the feasibility flow-sensitive alternating inversion recovery (FAIR) for the quantification of blood flow in lung parenchyma in free breathing rats at 7 T has been investigated. In order to obtain sufficient signal from the short T2 * lung parenchyma, a 2D ultra-short echo time (UTE) Look-Locker read-out has been implemented...
November 3, 2016: NMR in Biomedicine
https://www.readbyqxmd.com/read/27809255/mono-pegylation-of-alpha-mmc-and-map30-from-momordica-charantia-l-production-identification-and-anti-tumor-activity
#18
Yun Sun, Fenghui Sun, Jianlong Li, Minlu Wu, Xiang Fan, Yanfa Meng, Yao Meng
PEGylation is a well-established and effective strategy to decrease immunogenicity, which can increase the stability and in vivo half-life time. However, the generation of multi-site modified products is inevitable due to the lysine chemistry, which will bring difficulties in subsequent research, such as purification and quantification. Site-specific modification by mPEG-succinimidyl carbonate (mPEG-SC) is a widely used method for N-terminal conjugation. In this study, we used it for site-directed modification on two ribosome-inactivating proteins (RIPs), alpha-momorcharin (α-MMC) and momordica anti-HIV protein (MAP30), from Momordica charantia L...
October 31, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27805412/sod3-r213g-snp-blocks-murine-bleomycin-induced-fibrosis-and-promotes-resolution-of-inflammation
#19
Gary C Mouradian, Rohit Gaurav, Steve Pugliese, Karim El Kasmi, Brittany Hartman, Laura Hernandez-Lagunas, Kurt Stenmark, Russ P Bowler, Eva Nozik-Grayck
Loss of extracellular superoxide dismutase (SOD3) contributes to inflammatory and fibrotic lung diseases. The human SOD3 R213G polymorphism decreases matrix binding, redistributing SOD3 from the lung to extracellular fluids and protects against LPS induced alveolar inflammation. We utilized mice expressing a naturally occurring single nucleotide polymorphism (SNP), rs1799895, within the HBD of SOD3 which results in an amino acid substitution at position 213 (R213G mice) to test the hypothesis that the redistribution of SOD3 into the extracellular fluids would impart protection against bleomycin-induced lung fibrosis and secondary pulmonary hypertension (PH)...
November 2, 2016: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/27796893/roles-of-telocytes-in-the-development-of-angiogenesis
#20
Yonghua Zheng, Xiangdong Wang
Telocytes are cells with telopodes, which distinguish them from other interstitial cells. According to the study of lung, it was confirmed that telocytes were mainly distributed in the alveolar interstitial tissues connected tightly with alveolar epithelia cells and participated in the structure of air-blood barrier, in the small vein and bronchioles and in the interstitial space of smooth muscle participated in the frame structure of the blood and bronchioles. Telocytes are positive to CD34 and C-kit which expressed on the surface of hemopoietic stem cells, and are proposed to participate in the angiogenesis...
2016: Advances in Experimental Medicine and Biology
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