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https://www.readbyqxmd.com/read/29689441/development-and-validation-of-a-method-for-gefitinib-quantification-in-dried-blood-spots-using-liquid-chromatography-tandem-mass-spectrometry-application-to-finger-prick-clinical-blood-samples-of-patients-with-non-small-cell-lung-cancer
#1
Kei Irie, Saori Shobu, Seika Hiratsuji, Yuta Yamasaki, Shigeki Nanjo, Chiyuki Kokan, Akito Hata, Reiko Kaji, Katsuhiro Masago, Shiro Fujita, Yutaka Okada, Nobuyuki Katakami, Shoji Fukushima
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of gefitinib in dried blood spots (DBSs). Gefitinib was extracted with methanol from DBS of 3 mm in diameter and detected using a triple quadrupole mass spectrometer. The method was validated by evaluating its precision, accuracy, selectivity, carryover, matrix effect, recovery, and stability. For clinical validation, paired finger-prick DBS and plasma concentrations were compared for 10 patients with non-small cell lung cancer (NSCLC) taking gefitinib...
April 13, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29667549/dentification-and-quantification-of-quercetin-a-major-consitituent-of-artocarpus-altilis-by-targeting-related-genes-of-apoptosis-and-cell-cycle-in-vitro-cytotoxic-activity-against-human-lung-carcinoma-cell-lines-a549
#2
Silvana Russo, Walter Filgueira De Azevedo
BACKGROUND: Cannabinoid Receptor 1 (CB1) is a membrane protein prevalent in the central nervous system, whose crystallographic structure has recently been solved. Studies will be needed to investigate CB1 complexes with its ligands and its role in the development of new drugs. OBJECTIVE: Our goal here is to review the studies on CB1, starting with general aspects and focusing on the recent structural studies, with emphasis on the inverse agonists bound structures...
April 17, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29665769/sublethal-chronic-effects-of-oral-dietary-exposure-to-deltamethrin-in-swiss-albino-mice
#3
Anita Tewari, R S Banga, Jps Gill
The hazards of dietary exposure to environmentally relevant levels of deltamethrin are poorly understood though studies enunciate the acute toxicity hazards. In this study, prolonged exposure to low levels of deltamethrin in mice was investigated. The mice were exposed daily via gavage method for 60 days. Four doses (0.1, 0.05, 0.01, and 0.005 mg/kg bwt/d) were selected, which are equal to or less than the maximum residue limits for deltamethrin permitted in animal food/feed. Liver, kidney, lungs, spleen, and testes were collected on day 61 for histology, residue, and biochemical analysis (aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALKP), total bilirubin (TBIL), total proteins (TPs), cholesterol (CHOL), urea, and creatinine)...
January 1, 2018: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/29660899/plasma-exosome-levels-in-non-small-cell-lung-cancer-correlation-with-clinicopathological-features-and-prognostic-implications
#4
Qingyun Liu, Ying Xiang, Shuai Yuan, Weijia Xie, Chengying Li, Zeyao Hu, Ying Xiang, Na Wu, Long Wu, Zubin Yu, Li Bai, Yafei Li
BACKGROUND: Biomarker studies revealed important clinical significance of exosome for cancer patients. However, there is currently no consensus on exosome quantification methods. METHODS: Bicinchoninic acid (BCA) method, acetylcholinesterase (AChE) method and nanoparticle tracking analysis (NTA) were utilized to quantify 20 plasma exosome samples, and interrelations between these three methods were explored. Associations of plasma exosome levels with characteristics and prognosis of 208 non-small-cell lung cancer (NSCLC) patients were investigated...
April 3, 2018: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29660496/quantitative-targeted-proteomic-analysis-of-potential-markers-of-tyrosine-kinase-inhibitor-tki-sensitivity-in-egfr-mutated-lung-adenocarcinoma
#5
Shivangi Awasthi, Tapan Maity, Benjamin L Oyler, Yue Qi, Xu Zhang, David R Goodlett, Udayan Guha
Lung cancer causes the highest mortality among all cancers. Patients harboring kinase domain mutations in the epidermal growth factor receptor (EGFR) respond to EGFR tyrosine kinase inhibitors (TKIs), however, acquired resistance always develops. Moreover, 30-40% of patients with EGFR mutations exhibit primary resistance. Hence, there is an unmet need for additional biomarkers of TKI sensitivity that complement EGFR mutation testing and predict treatment response. We previously identified phosphopeptides whose phosphorylation is inhibited upon treatment with EGFR TKIs, erlotinib and afatinib in TKI sensitive cells, but not in resistant cells...
April 13, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29659138/quantification-of-airway-fibrosis-in-asthma-by-flow-cytometry
#6
Andrew Reichard, Nicholas Wanner, Eric Stuehr, Mario Alemagno, Kelly Weiss, Kimberly Queisser, Serpil Erzurum, Kewal Asosingh
Airway fibrosis is a prominent feature of asthma, contributing to the detrimental consequences of the disease. Fibrosis in the airway is the result of collagen deposition in the reticular lamina layer of the subepithelial tissue. Myofibroblasts are the leading cell type involved with this collagen deposition. Established methods of collagen deposition quantification present various issues, most importantly their inability to quantify current collagen biosynthesis occurring in airway myofibroblasts. Here, a novel method to quantify myofibroblast collagen expression in asthmatic lungs is described...
April 16, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29644247/quantification-of-torque-teno-virus-and-epstein-barr-virus-is-of-limited-value-for-predicting-the-net-state-of-immunosuppression-after-lung-transplantation
#7
Rickard Nordén, Jesper Magnusson, Anna Lundin, Ka-Wei Tang, Staffan Nilsson, Magnus Lindh, Lars-Magnus Andersson, Gerdt C Riise, Johan Westin
Background: Major hurdles for survival after lung transplantation are rejections and infectious complications. Adequate methods for monitoring immune suppression status are lacking. Here, we evaluated quantification of torque teno virus (TTV) and Epstein-Barr virus (EBV) as biomarkers for defining the net state of immunosuppression in lung-transplanted patients. Methods: This prospective single-center study included 98 patients followed for 2 years after transplantation...
April 2018: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/29624708/accuracy-of-electron-density-effective-atomic-number-and-iodine-concentration-determination-with-a-dual-layer-dual-energy-computed-tomography-system
#8
Chia-Ho Hua, Nadav Shapira, Thomas E Merchant, Paul Klahr, Yoad Yagil
PURPOSE: This study aimed to quantify the accuracy of the determination of electron density (ED), effective atomic number (Zeff ), and iodine concentration, directed for more accurate radiation therapy planning, with a new dual-layer dual-energy computed tomography (DL-DECT) system. The dependence of the accuracy of these values on the scan and reconstruction parameters, as well as on the phantom size, was also examined. METHODS: Measurements were performed on a commercial DECT system with a DL detector (IQon Spectral CT, Philips Healthcare), using phantoms with various tissue-equivalent inserts as well as iodine and calcium inserts of different concentrations...
April 6, 2018: Medical Physics
https://www.readbyqxmd.com/read/29611767/impulse-oscillometry-and-nitrogen-washout-test-in-the-assessment-of-small-airway-dysfunction-in-asthma-correlation-with-quantitative-computed-tomography
#9
Leonello Fuso, Giuseppe Macis, Carola Condoluci, Martina Sbarra, Chiara Contu, Emanuele G Conte, Giulia Angeletti, Paolo Montuschi
OBJECTIVE: Small airway dysfunction (SAD) and airway remodeling influence the disease control and progression in asthma. We investigated whether impulse oscillometry (IOS) and single breath nitrogen washout (SBN2 W) could be reliable tests in evaluating SAD and airway remodeling by correlating their data with radiological parameters derived from quantitative chest multidetector computed tomography (MDCT) imaging. METHODS: Lung function tests were performed before and after bronchodilator...
April 3, 2018: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/29607606/detection-of-chronic-lung-allograft-dysfunction-using-ventilation-weighted-fourier-decomposition-mri
#10
A Voskrebenzev, M Greer, M Gutberlet, C Schönfeld, J Renne, J Hinrichs, T Kaireit, T Welte, F Wacker, J Gottlieb, J Vogel-Claussen
Chronic lung allograft dysfunction (CLAD) remains the leading cause of morbidity and mortality after lung transplantation. Diagnosis requires spirometric change, that becomes increasingly difficult with advancing CLAD. Fourier decomposition MRI (FD-MRI) permits acquisition of ventilated-weighted images during free-breathing. This study evaluates FD-MRI in detecting CLAD in selected patients after bilateral lung transplantation (DLTx). DLTx recipients demonstrating CLAD at various stages participated. Radiologists remained blinded to clinical status until completion of image analysis...
April 1, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29603531/a-new-multiplex-real-time-taqman-%C3%A2-pcr-for-quantification-of-mycoplasma-hyopneumoniae-m-hyorhinis-and-m-flocculare-exploratory-epidemiological-investigations-to-research-mycoplasmal-association-in-enzootic-pneumonia-like-lesions-in-slaughtered-pigs
#11
Sarah Fourour, Christelle Fablet, Véronique Tocqueville, Virginie Dorenlor, Florent Eono, Eric Eveno, Isabelle Kempf, Corinne Marois-Créhan
AIMS: A new multiplex qPCR targeting Mycoplasma (M.) hyopneumoniae, M. hyorhinis and M. flocculare was developed and the relationship between the detection of those mycoplasma species and the extent of gross pneumonia like lesions in slaughtered pigs lungs were investigated. METHODS AND RESULTS: The multiplex qPCR method targets the p102, p37 and fruA genes and has detection limits of 14, 146, and 16 genome equivalents μl-1 for M. hyopneumoniae, M. hyorhinis and M...
March 30, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29600976/impact-of-endostatin-gene-therapy-on-myeloid-derived-suppressor-cells-from-a-metastatic-renal-cell-carcinoma
#12
K C Chaves, E M Costa, L F Teixeira, M H Bellini
AIM: To evaluate the role of endostatin (ES) gene therapy on myeloid-derived suppressor cells (MDSC) in a metastatic model of renal cell carcinoma (RCC). MATERIALS AND METHODS: Balb/C mice bearing orthotopic Renca tumors were treated with NIH/3T3-LendSN or, as a control, with NIH/3T3-LXSN cells. At the end of in vivo experiment, plasma and tissue lung samples were collected. Plasma ES and granulocyte colony stimulating factor (G-CSF) levels were measured by ELISA and Milliplex, respectively...
March 2018: Experimental Oncology
https://www.readbyqxmd.com/read/29598865/pet-assessment-of-epicardial-intimal-disease-and-microvascular-dysfunction-in-cardiac-allograft-vasculopathy
#13
Sharon Chih, Aun Yeong Chong, Fernanda Erthal, Robert A deKemp, Ross A Davies, Ellamae Stadnick, Derek Y So, Christopher Overgaard, George Wells, Lisa M Mielniczuk, Rob S B Beanlands
BACKGROUND: Cardiac allograft vasculopathy (CAV) is a leading cause of graft failure and death after heart transplantation. Absolute myocardial blood flow (MBF) quantification using rubidium 82 (Rb-82) positron emission tomography (PET) could enable evaluation of diagnostically challenging diffuse epicardial and microvascular disease in CAV. OBJECTIVES: The authors aimed to evaluate Rb-82 PET detection of CAV. METHODS: Consecutive transplant recipients undergoing coronary angiography were prospectively evaluated with PET, multivessel intravascular ultrasound (IVUS), and intracoronary hemodynamics...
April 3, 2018: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/29595754/the-importance-and-quantification-of-plutonium-binding-in-human-lungs
#14
Alan Birchall, Matthew Puncher, Alan Hodgson, Sergei Y Tolmachev
Epidemiological studies have shown that the main risk arising from exposure to plutonium aerosols is lung cancer, with other detrimental effects in the bone and liver. A realistic assessment of these risks, in turn, depends on the accuracy of the dosimetric models used to calculate doses in such studies. A state-of-the-art biokinetic model for plutonium, based on the current International Commission on Radiological Protection biokinetic model, has been developed for this purpose in an epidemiological study involving the plutonium exposure of Mayak workers in Ozersk, Russia...
March 28, 2018: Health Physics
https://www.readbyqxmd.com/read/29582202/automated-quantitative-image-analysis-for-ex-vivo-metastasis-assays-reveals-differing-lung-composition-requirements-for-metastasis-suppression-by-kiss1
#15
Eric D Young, Kyle Strom, Ashley F Tsue, Joseph L Usset, Seth MacPherson, John T McGuire, Danny R Welch
Imaging is broadly used in biomedical research, but signal variation complicates automated analysis. Using the Pulmonary Metastasis Assay (PuMA) to study metastatic colonization by the metastasis suppressor KISS1, we cultured GFP-expressing melanoma cells in living mouse lung ex vivo for 3 weeks. Epifluorescence images of cells were used to measure growth, creating large datasets which were time consuming and challenging to quantify manually due to scattering of light from outside the focal plane. To address these challenges, we developed an automated workflow to standardize the measurement of disseminated cancer cell growth by applying statistical quality control to remove unanalyzable images followed and a filtering algorithm to quantify only in-focus cells...
March 26, 2018: Clinical & Experimental Metastasis
https://www.readbyqxmd.com/read/29578522/histological-quantification-to-determine-lung-fungal-burden-in-experimental-aspergillosis
#16
Dylan J Stolz, Ethan M Sands, Nansalmaa Amarsaikhan, Angar Tsoggerel, Steven P Templeton
The quantification of lung fungal burden is critical for the determination of the relative levels of immune protection and fungal virulence in mouse models of pulmonary fungal infection. Although multiple methods are used to assess fungal burden, quantitative polymerase chain reaction (qPCR) of fungal DNA has emerged as a technique with several advantages over previous culture-based methods. Currently, a comprehensive assessment of lung pathology, leukocyte recruitment, fungal burden, and gene expression in mice with invasive aspergillosis (IA) necessitates the use of a significant number of experimental and control animals...
March 9, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29577352/retinoic-acid-and-tracheal-occlusion-for-diaphragmatic-hernia-treatment-in-rabbit-fetuses
#17
Amélie Delabaere, Loïc Blanchon, Karen Coste, Gael Clairefond, Corinne Belville, Pierre Blanc, Geoffroy Marceau, Vincent Sapin, Denis Gallot
INTRODUCTION: Lung hypoplasia and pulmonary arterial hypertension (PAH) in congenital diaphragmatic hernia (DH) lead to a high perinatal mortality. Although sustained fetoscopic tracheal occlusion (TO) improves lung development, a major side effect is abnormal pneumocyte differentiation. This study evaluated the potential ability of intra-tracheal retinoic acid (RA) administration to reduce adverse effects of sustained TO in a rabbit model of DH. METHODS: A left diaphragmatic defect was created on d23 in time-dated pregnant rabbits...
March 25, 2018: Prenatal Diagnosis
https://www.readbyqxmd.com/read/29564709/oral-exposure-of-deltamethrin-and-or-lipopolysaccharide-lps-induced-activation-of-the-pulmonary-immune-system-in-swiss-albino-mice
#18
Anita Tewari, Jasbir Bedi, Baljit Singh, Jatinder Paul Singh Gill
The deltamethrin, a synthetic pyrethroid, is used worldwide and has been linked with several type of acute toxicity. However, effect of low level of deltamethrin alone or in combination with the microbial antigen on pulmonary system is not understood. Lipopolysaccharide (LPS) was used as antigen which is a key inflammatory component of gram-negative bacteria, which induces a distinctive pattern of cytokine release that regulates inflammation. The aim was to determine whether chronic exposure to a low level of deltamethrin alone or in combination with LPS impair the lung response in adult male Swiss albino mice...
March 22, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29553533/imaging-mycobacterium-tuberculosis-in-mice-with-reporter-enzyme-fluorescence
#19
Riti Sharan, Hee-Jeong Yang, Preeti Sule, Jeffrey D Cirillo
Reporter enzyme fluorescence (REF) utilizes substrates that are specific for enzymes present in target organisms of interest for imaging or detection by fluorescence or bioluminescence. We utilize BlaC, an enzyme expressed constitutively by all M. tuberculosis strains. REF allows rapid quantification of bacteria in lungs of infected mice. The same group of mice can be imaged at many time points, greatly reducing costs, enumerating bacteria more quickly, allowing novel observations in host-pathogen interactions, and increasing statistical power, since more animals per group are readily maintained...
February 26, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29552855/-flavor-and-meridian-tropism-classification-analysis-of-callianthemum-taipaicum
#20
Yan-Ru Liu, Zhi-Shu Tang, Jin-Ao Duan, Xiao-Chun Sun, Zhong-Xing Song, Xin-Bo Shi, Yang Lv, Zhao Zhang, Da-Wei Qian
To explore the flavor and meridian tropism classification of Callianthemum taipaicum by principal components analysis(PCA) and partial least square analysis(PLS). Meanwhile,to establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-ESI-MS) method for the simultaneous determination of 55 active components from 13 kinds of Ranunculaceae of Chinese traditional herbs. Samples were separated on HPLC system by Agilent 5 TC-C₁₈(2)(4.6 mm×250 mm,5 μm)column and eluted with acetonitrile and 0...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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