keyword
https://read.qxmd.com/read/38132815/titanium-biohybrid-middle-ear-prostheses-a-preliminary-in-vitro-study
#21
JOURNAL ARTICLE
Mario Mosconi, Elena Carlotto, Laura Caliogna, Micaela Berni, Giulia Gastaldi, Michele Conti, Alice Maria Brancato, Valentina Bina, Domenico Minervini, Stefano Malpede, Anna Chiara Stellato, Francesco Lazzerini, Luca Bruschini, Marco Benazzo, Pietro Canzi
Ossiculoplasty is a surgical operation performed to restore auditory transmission through the reconstruction of the ossicular chain using prosthetics. Tissue bioengineering has assumed a pivotal role in implementing alternatives to conventional ossicular middle ear replacement prostheses, to overcome extrusion while preserving acoustic properties. This in vitro study aims to explore, for the first time in current literature, the feasibility of a biohybrid middle ear prosthesis, composed of titanium surrounded by a bone extracellular matrix as bio-coating...
November 29, 2023: Journal of Functional Biomaterials
https://read.qxmd.com/read/38128539/an-essential-signaling-cascade-for-avian-auditory-hair-cell-regeneration
#22
JOURNAL ARTICLE
Nesrine Benkafadar, Mitsuo P Sato, Angela H Ling, Amanda Janesick, Mirko Scheibinger, Taha A Jan, Stefan Heller
Hearing loss is a chronic disease affecting millions of people worldwide, yet no restorative treatment options are available. Although non-mammalian species can regenerate their auditory sensory hair cells, mammals cannot. Birds retain facultative stem cells known as supporting cells that engage in proliferative regeneration when surrounding hair cells die. Here, we investigated gene expression changes in chicken supporting cells during auditory hair cell death. This identified a pathway involving the receptor F2RL1, HBEGF, EGFR, and ERK signaling...
December 15, 2023: Developmental Cell
https://read.qxmd.com/read/38123643/embryonic-stem-cell-derived-mesenchymal-stem-cells-relieve-experimental-contact-urticaria-by-regulating-the-functions-of-mast-cells-and-t-cells
#23
JOURNAL ARTICLE
Seung Yeun Hyun, Eun-Young Kim, Minseong Kang, Jeong Won Park, Ki-Sung Hong, Hyung-Min Chung, Wahn Soo Choi, Se-Pill Park, Geunwoong Noh, Hyuk Soon Kim
Contact urticaria (CU) is an inflammatory skin disorder triggered by specific substances upon skin contact, leading to immediate acute or chronic manifestations characterized by swelling and redness. While mesenchymal stem cells (MSCs) are increasingly recognized for their therapeutic potential in immune diseases, research on the efficacy and mechanisms of stem cell therapy for urticaria remains scarce. This study investigates the regulatory role of embryonic-stem-cell-derived multipotent MSCs (M-MSCs) administered in a CU mouse model...
December 20, 2023: Scientific Reports
https://read.qxmd.com/read/38087407/isolation-and-characterization-of-a-novel-alpha-hemolytic-streptococcus-spp-from-the-oral-cavity-and-blood-of-septicemic-periparturient-immunodeficient-mice
#24
JOURNAL ARTICLE
Amy Funk, Qidong Jia, Laura Janke, Ashley Crawford, Amy Iverson, Jason Rosch, Joseph Emmons, Chandra Savage, Heather Glasgow, Randall Hayden, Elisa Margolis, Harshan Pisharath
MISTRG is an immunodeficient mouse strain that expresses multiple human cytokines that support hematopoietic stem cell maintenance and myelopoiesis. While establishing a breeding colony of MISTRG mice in a dedicated barrier room, 6 cases of death or disease occurred in pregnant or postpartum mice. Clinically, this manifested as hunched posture, dyspnea, and 1 case of emaciation with ataxia. Pathologic analysis of 7 mice revealed multisystemic necrosuppurative inflammation variably affecting the uterus and placenta, joints, meninges, inner and middle ears, kidneys, and small intestine...
October 29, 2023: Comparative Medicine
https://read.qxmd.com/read/38052294/in-vitro-modeling-of-cranial-placode-differentiation-recent-advances-challenges-and-perspectives
#25
REVIEW
Casey Griffin, Jean-Pierre Saint-Jeannet
Cranial placodes are transient ectodermal thickenings that contribute to a diverse array of organs in the vertebrate head. They develop from a common territory, the pre-placodal region that over time segregates along the antero-posterior axis into individual placodal domains: the adenohypophyseal, olfactory, lens, trigeminal, otic, and epibranchial placodes. These placodes terminally differentiate into the anterior pituitary, the lens, and contribute to sensory organs including the olfactory epithelium, and inner ear, as well as several cranial ganglia...
December 3, 2023: Developmental Biology
https://read.qxmd.com/read/38020205/photobiomodulation-can-enhance-stem-cell-viability-in-cochlea-with-auditory-neuropathy-but-does-not-restore-hearing
#26
JOURNAL ARTICLE
So-Young Chang, Eunjeong Kim, Nathaniel T Carpena, Jae-Hun Lee, Doo Hee Kim, Min Young Lee
Sensorineural hearing loss is very difficult to treat. Currently, one of the techniques used for hearing rehabilitation is a cochlear implant that can transform sound into electrical signals instead of inner ear hair cells. However, the prognosis remains very poor if sufficient auditory nerve cells are not secured. In this study, the effect of mouse embryonic stem cells (mESC) and photobiomodulation (PBM) combined treatment on auditory function and auditory nerve cells in a secondary neuropathy animal model was investigated...
2023: Stem Cells International
https://read.qxmd.com/read/38004094/polyphenols-in-inner-ear-neurobiology-health-and-disease-from-bench-to-clinics
#27
REVIEW
Naomi Osakabe, Sergio Modafferi, Maria Laura Ontario, Francesco Rampulla, Vincenzo Zimbone, Maria Rita Migliore, Tilman Fritsch, Ali S Abdelhameed, Luigi Maiolino, Gabriella Lupo, Carmelina Daniela Anfuso, Elisabetta Genovese, Daniele Monzani, Uwe Wenzel, Edward J Calabrese, R Martin Vabulas, Vittorio Calabrese
There is substantial experimental and clinical interest in providing effective ways to both prevent and slow the onset of hearing loss. Auditory hair cells, which occur along the basilar membrane of the cochlea, often lose functionality due to age-related biological alterations, as well as from exposure to high decibel sounds affecting a diminished/damaged auditory sensitivity. Hearing loss is also seen to take place due to neuronal degeneration before or following hair cell destruction/loss. A strategy is necessary to protect hair cells and XIII cranial/auditory nerve cells prior to injury and throughout aging...
November 20, 2023: Medicina
https://read.qxmd.com/read/37992123/bmp9-is-a-potent-inducer-of-chondrogenesis-volumetric-expansion-and-collagen-type-ii-accumulation-in-bovine-auricular-cartilage-chondroprogenitors
#28
JOURNAL ARTICLE
Oliver F W Gardner, Yadan Zhang, Ilyas M Khan
Reconstruction of the outer ear currently requires harvesting of cartilage from the posterior of the auricle or ribs leading to pain and donor site morbidity. An alternative source for auricular reconstruction is in vitro tissue engineered cartilage using stem/progenitor cells. Several candidate cell-types have been studied with tissue-specific auricular cartilage progenitor cells (AuCPC) of particular interest. Whilst chondrogenic differentiation of competent stem cells using growth factor TGFβ1 produces cartilage this tissue is frequently fibrocartilaginous and lacks the morphological features of hyaline cartilage...
2023: PloS One
https://read.qxmd.com/read/37986843/ectopic-adipogenesis-in-response-to-injury-and-material-implantation-in-an-autoimmune-mouse-model
#29
Tran B Ngo, Aditya Josyula, Sabrina DeStefano, Daphna Fertil, Mondreakest Faust, Ravi Lokwani, Kaitlyn Sadtler
Due to the limited capacity of mammals to regenerate complex tissues, researchers have worked to understand the mechanisms of tissue regeneration in organisms that maintain that capacity. One example is the MRL/MpJ mouse strain with unique regenerative capacity in ear pinnae that is absent from other strains, such as the common C57BL/6 strain. The MRL/MpJ mouse has also been associated with an autoimmune phenotype even in the absence of the mutant Fas gene described in its parent strain MRL/lpr. Due to these findings, we evaluated the differences between the responses of MRL/MpJ versus C57BL/6 strain in traumatic muscle injury and subsequent material implantation...
October 7, 2023: bioRxiv
https://read.qxmd.com/read/37984376/genotyping-protocols-for-genetically-engineered-mice
#30
JOURNAL ARTICLE
Advait Limaye, Kyoungin Cho, Bradford Hall, Jaspal S Khillan, Ashok B Kulkarni
Historically, the laboratory mouse has been the mammalian species of choice for studying gene function and for modeling diseases in humans. This was mainly due to their availability from mouse fanciers. In addition, their short generation time, small size, and minimal food consumption compared to that of larger mammals were definite advantages. This led to the establishment of large hubs for the development of genetically modified mouse models, such as the Jackson Laboratory. Initial research into inbred mouse strains in the early 1900s revolved around coat color genetics and cancer studies, but gene targeting in embryonic stem cells and the introduction of transgenes through pronuclear injection of a mouse zygote, along with current clustered regularly interspaced short palindromic repeat (CRISPR) RNA gene editing, have allowed easy manipulation of the mouse genome...
November 2023: Current protocols
https://read.qxmd.com/read/37939851/bdnf-enriched-small-extracellular-vesicles-protect-against-noise-induced-hearing-loss-in-mice
#31
JOURNAL ARTICLE
Xin Min, Xiao-Hui Deng, Huilin Lao, Zi-Cong Wu, Yi Chen, Yuelian Luo, Haoyang Wu, Junbo Wang, Qing-Ling Fu, Hao Xiong
Noise-induced hearing loss (NIHL) is one of the most prevalent acquired sensorineural hearing loss etiologies and is characterized by the loss of cochlear hair cells, synapses, and nerve terminals. Currently, there are no agents available for the treatment of NIHL because drug delivery to the inner ear is greatly limited by the blood-labyrinth barrier (BLB). In this study, we used mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) as nanoscale vehicles to deliver brain-derived neurotrophic factor (BDNF) and evaluated their protective effects in a mouse model of NIHL...
November 6, 2023: Journal of Controlled Release
https://read.qxmd.com/read/37917115/unraveling-the-mechanisms-of-vestibular-neuron-formation-from-human-induced-pluripotent-stem-cells
#32
JOURNAL ARTICLE
Benjamin M Norton, Analia N Quirk, Akihiro J Matsuoka
The development of in vitro models that accurately recapitulate the complex cellular and molecular interactions of the inner ear is crucial for understanding inner ear development, function, and disease. In this study, we utilized a customized microfluidic platform to generate human induced pluripotent stem cell (hiPSC)-derived 3D otic sensory neurons (OSNs). Careful modulation of Wnt and Shh signaling pathways was used to direct differentiation toward a vestibular neuron lineage. hiPSC-derived otic neuronal progenitors were cultured in hydrogel-embedded microfluidic channels over a 40-day period...
November 2, 2023: Tissue Engineering. Part A
https://read.qxmd.com/read/37847285/permanent-occlusion-of-the-eustachian-tube-a-retrospective-study-on-reopening-procedures
#33
JOURNAL ARTICLE
Holger Sudhoff
PURPOSE: This study retrospectively evaluated the efficacy and versatility of reopening procedures for the permanent occlusion of the cartilaginous Eustachian tube (POET) by analyzing four consecutive cases. METHODS: The study included all patients diagnosed with POET who suffered from Eustachian tube occlusion and glue ear. A combined approach of endoscopic transnasal/transoral laser surgery was utilized to reopen the POET. This was subsequently followed by balloon dilation (BET) and stenting for a duration of six weeks...
October 17, 2023: European Archives of Oto-rhino-laryngology
https://read.qxmd.com/read/37845538/-organoids-the-key-to-novel-therapies-for-the-inner-ear-german-version
#34
REVIEW
Marc Diensthuber, Timo Stöver
The sensitivity and the complexity of the human inner ear in conjunction with the lack of regenerative capacity are the main reasons for hearing loss and tinnitus. Progress in the development of protective and regenerative therapies for the inner ear often failed in the past not least due to the fact that no suitable model systems for cell biological and pharmacological in vitro studies were available. A novel technology for creating "mini-organs", so-called organoids, could solve this problem and has now also reached inner ear research...
October 16, 2023: HNO
https://read.qxmd.com/read/37796037/mapping-oto-pharyngeal-development-in-a-human-inner-ear-organoid-model
#35
JOURNAL ARTICLE
Matthew R Steinhart, Wouter H van der Valk, Daniel Osorio, Sara A Serdy, Jingyuan Zhang, Carl Nist-Lund, Jin Kim, Cynthia Moncada-Reid, Liang Sun, Jiyoon Lee, Karl R Koehler
Inner ear development requires the coordination of cell types from distinct epithelial, mesenchymal and neuronal lineages. Although we have learned much from animal models, many details about human inner ear development remain elusive. We recently developed an in vitro model of human inner ear organogenesis using pluripotent stem cells in a 3D culture, fostering the growth of a sensorineural circuit, including hair cells and neurons. Despite previously characterizing some cell types, many remain undefined. This study aimed to chart the in vitro development timeline of the inner ear organoid to understand the mechanisms at play...
October 1, 2023: Development
https://read.qxmd.com/read/37791525/human-pluripotent-stem-cells-derived-inner-ear-organoids-recapitulate-otic-development-in-vitro
#36
JOURNAL ARTICLE
Daniela Doda, Sara Alonso Jimenez, Hubert Rehrauer, Jose F Carreño, Victoria Valsamides, Stefano Di Santo, Hans Ruedi Widmer, Albert Edge, Heiko Locher, Wouter van der Valk, Jingyuan Zhang, Karl R Koehler, Marta Roccio
Our molecular understanding of the early stages of human inner ear development has been limited by the difficulty in accessing fetal samples at early gestational stages. As an alternative, previous studies have shown that inner ear morphogenesis can be partially recapitulated using induced pluripotent stem cells (iPSCs) directed to differentiate into Inner Ear Organoids (IEOs). Once validated and benchmarked, these systems could represent unique tools to complement and refine our understanding of human otic differentiation and model developmental defects...
August 10, 2023: Development
https://read.qxmd.com/read/37787875/stem-cell-based-hair-cell-regeneration-and-therapy-in-the-inner-ear
#37
REVIEW
Jieyu Qi, Wenjuan Huang, Yicheng Lu, Xuehan Yang, Yinyi Zhou, Tian Chen, Xiaohan Wang, Yafeng Yu, Jia-Qiang Sun, Renjie Chai
Hearing loss has become increasingly prevalent and causes considerable disability, thus gravely burdening the global economy. Irreversible loss of hair cells is a main cause of sensorineural hearing loss, and currently, the only relatively effective clinical treatments are limited to digital hearing equipment like cochlear implants and hearing aids, but these are of limited benefit in patients. It is therefore urgent to understand the mechanisms of damage repair in order to develop new neuroprotective strategies...
January 2024: Neuroscience Bulletin
https://read.qxmd.com/read/37746148/stem-cells-as-potential-therapeutics-for-hearing-loss
#38
REVIEW
Qiaojun Fang, Yongjie Wei, Yuhua Zhang, Wei Cao, Lin Yan, Mengdie Kong, Yongjun Zhu, Yan Xu, Lingna Guo, Lei Zhang, Weiqing Wang, Yafeng Yu, Jingwu Sun, Jianming Yang
Hearing impairment is a global health problem. Stem cell therapy has become a cutting-edge approach to tissue regeneration. In this review, the recent advances in stem cell therapy for hearing loss have been discussed. Nanomaterials can modulate the stem cell microenvironment to augment the therapeutic effects further. The potential of combining nanomaterials with stem cells for repairing and regenerating damaged inner ear hair cells (HCs) and spiral ganglion neurons (SGNs) has also been discussed. Stem cell-derived exosomes can contribute to the repair and regeneration of damaged tissue, and the research progress on exosome-based hearing loss treatment has been summarized as well...
2023: Frontiers in Neuroscience
https://read.qxmd.com/read/37745294/grainyhead-like-2-is-required-for-morphological-integrity-of-mouse-embryonic-stem-cells-and-orderly-formation-of-inner-ear-like-organoids
#39
JOURNAL ARTICLE
Blaise Forrester-Gauntlett, Linda Peters, Björn Oback
Mutations in the transcription factor gene grainyhead-like 2 ( GRHL2 ) are associated with progressive non-syndromic sensorineural deafness autosomal dominant type 28 ( DFNA28 ) in humans. Since complete loss of Grhl2 is lethal in mouse embryos, we studied its role during inner ear pathology and hearing loss in vitro . To this end, we generated different homozygous deletions to knockout Grhl2 in mouse embryonic stem cells ( Grhl2-KO ESCs), including some mimicking naturally occurring truncations in the dimerisation domain related to human DFNA28 ...
2023: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/37737178/induced-pluripotent-stem-cells-ex-vivo-models-for-human-diseases-due-to-mitochondrial-dna-mutations
#40
REVIEW
Chao Chen, Min-Xin Guan
Mitochondria are essential organelles for cellular metabolism and physiology in eukaryotic cells. Human mitochondria have their own genome (mtDNA), which is maternally inherited with 37 genes, encoding 13 polypeptides for oxidative phosphorylation, and 22 tRNAs and 2 rRNAs for translation. mtDNA mutations are associated with a wide spectrum of degenerative and neuromuscular diseases. However, the pathophysiology of mitochondrial diseases, especially for threshold effect and tissue specificity, is not well understood and there is no effective treatment for these disorders...
September 22, 2023: Journal of Biomedical Science
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