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https://www.readbyqxmd.com/read/27927929/high-throughput-next-generation-sequencing-of-polioviruses
#1
Anna M Montmayeur, Terry Fei Fan Ng, Alexander Schmidt, Kun Zhao, Laura Magaña, Jane Iber, Christina J Castro, Qi Chen, Elizabeth Henderson, Edward Ramos, Jing Shaw, Roman L Tatusov, Naomi Dybdahl-Sissoko, Marie Claire Endegue-Zanga, Johnson A Adeniji, M Steven Oberste, Cara C Burns
Poliovirus (PV) is currently targeted for worldwide eradication and containment. Sanger-based sequencing of the VP1 capsid region is the current standard method for PV surveillance; however, the whole genome sequence is sometimes needed for higher resolution global surveillance. In this study, we optimized whole genome sequencing protocols for poliovirus isolates and FTA cards using NGS, aiming for high sequence coverage, efficiency, and throughput. We found that DNase treatment of poliovirus RNA followed by random RT, amplification, and the Nextera XT DNA Library Preparation Kit produced significantly better results than other preparations...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27918737/influence-of-bethanechol-on-salivary-parameters-in-irradiated-patients
#2
C Carrara-Cotomacio, L Campos, A Simões, G Chagas-Jaguar, E-M Crosato, F Abreu-Alves
BACKGROUND: Some studies have shown evidence that the prophylactic use of bethanechol chloride (BC) may be useful in preventing the incidence and/or severity of xerostomia (XT). However, the indication of BC in irradiated patients with XT needs to be better characterized. The study aimed to evaluate the influence of BC on XT, salivary flow rate, and salivary composition in patients previously submitted to head and neck radiotherapy. MATERIAL AND METHODS: Forty five irradiated patients complaining of XT used 50 mg/day of BC for 3 months, and the salivary parameters were evaluated in 4 Phases (Before BC therapy, after one month of BC, 2 months of BC, and 3 months of BC)...
December 6, 2016: Medicina Oral, Patología Oral y Cirugía Bucal
https://www.readbyqxmd.com/read/27916776/favorable-clinical-outcomes-of-transcatheter-aortic-valve-implantation-in-japanese-patients%C3%A3-first-report-from-the-post-approval-k-tavi-registry
#3
Shinya Takimoto, Naritatsu Saito, Kenji Minakata, Shinichi Shirai, Akihiro Isotani, Yoshio Arai, Michiya Hanyu, Tatsuhiko Komiya, Takeshi Shimamoto, Tsuyoshi Goto, Yasushi Fuku, Natsuhiko Ehara, Yutaka Furukawa, Tadaaki Koyama, Atsuhi Nagasawa, Toshihiro Tamura, Makoto Miyake, Kazuo Yamanaka, Hisashi Sakaguchi, Koichiro Murata, Tomoya Onodera, Fumio Yamazaki, Masanao Nakai, Tomohiko Taniguchi, Ryuzo Sakata, Takeshi Kimura
BACKGROUND: Very limited data exist on the outcomes of transcatheter aortic valve implantation (TAVI) since Japanese marketing approval of the first TAVI device.Methods and Results:The Kyoto University-related hospital Transcatheter Aortic Valve Implantation (K-TAVI) registry includes prospectively collected data from 6 participating hospitals in Japan. We included 302 patients with severe aortic stenosis who underwent TAVI using the SAPIEN XT balloon-expandable valve via transfemoral (TF; n=203, 67%) or transapical (TA; n=99, 33%) approach between October 2013 and September 2015...
December 3, 2016: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/27903258/catwalk-gait-analysis-in-a-rat-model-of-multiple-sclerosis
#4
Sabine Herold, Prateek Kumar, Klaus Jung, Irina Graf, Henrike Menkhoff, Xenia Schulz, Mathias Bähr, Katharina Hein
BACKGROUND: Myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) is a widely used animal model for multiple sclerosis. The characteristic feature of the MOG-EAE model in Brown Norway rats is consistent involvement of the spinal cord resulting in limb paresis. The aim of the study was to investigate whether early subclinical gait abnormalities are present in this animal model and can be detected by CatWalk XT, a fully automated gait analysis system...
November 30, 2016: BMC Neuroscience
https://www.readbyqxmd.com/read/27899140/transapical-transcatheter-aortic-valve-replacement-in-patients-with-or-without-prior-coronary-artery-bypass-graft-operation
#5
Konstantinos V Voudris, S Chiu Wong, Ryan Kaple, Polydoros N Kampaktsis, Andreas R de Biasi, Jonathan S Weiss, Richard Devereux, Karl Krieger, Luke Kim, Rajesh V Swaminathan, Dmitriy N Feldman, Harsimran Singh, Nikolaos J Skubas, Robert M Minutello, Geoffrey Bergman, Arash Salemi
BACKGROUND: Transapical approach (TA) is an established access alternative to the transfemoral technique in patients undergoing transcatheter aortic valve replacement (TAVR) for treatment of symptomatic aortic valve stenosis. The impact of prior coronary artery bypass grafting (CABG) on clinical outcomes in patients undergoing TA-TAVR is not well defined. METHODS: A single center retrospective cohort analysis of 126 patients (male 41%, mean age 85.8 ± 6.1 years) who underwent TA balloon expandable TAVR (Edwards SAPIEN, SAPIEN XT or SAPIEN 3) was performed...
November 29, 2016: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/27896419/effects-of-enamel-sealing-on-shear-bond-strength-and-the-adhesive-remnant-index-study-of-three-fluoride-releasing-adhesives-in-combination-with-metal-and-ceramic-brackets
#6
Elisabeth Hofmann, Laura Elsner, Ursula Hirschfelder, Thomas Ebert, Sebastian Hanke
OBJECTIVES: Selected combinations of materials were used to create tooth-adhesive-bracket complexes to evaluate shear bond strength (SBS) and the adhesive remnant index (ARI) with regard to enamel sealing. METHODS: Four adhesive systems also appropriate for use as enamel sealants were combined with four bracket types, resulting in 16 adhesive-bracket combinations, each of which was tested on 15 permanent bovine incisors. Sealant-adhesives included two recently introduced fluoride-releasing systems (Riva bond LC(®) and go!(®)), one established primer (Opal(®) Seal™), and one commonly used adhesive as control (Transbond™ XT)...
November 28, 2016: Journal of Orofacial Orthopedics, Fortschritte der Kieferorthopädie
https://www.readbyqxmd.com/read/27893355/performance-evaluation-study-of-id-core-xt-a-high-throughput-blood-group-genotyping-platform
#7
Mónica López, Izaskun Apraiz, Montserrat Rubia, Mercedes Piedrabuena, Maria Azkarate, Barbera Veldhuisen, Miguel Á Vesga, Ellen Van Der Schoot, Fernando Puente, Diego Tejedor
BACKGROUND: Traditionally, red blood cell antigens have been identified using serological methods, but recent advances in molecular biology have made the implementation of methods for genetic testing of most blood group antigens possible. The goal of this study was to validate the performance of the ID CORE XT blood group typing assay. MATERIALS AND METHODS: One thousand independent samples from donors, patients and neonates were collected from three research institutes in Spain and the Netherlands...
November 25, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27891456/effect-of-salivary-ph-on-color-stability-of-different-flowable-composites-a-prospective-in-vitro-study
#8
Renu Batra, Pratik Kataria, Sonali Kapoor
INTRODUCTION: Scientifically and clinically there has been lot of development in the field of aesthetic dentistry. However, there is limited or restricted information regarding the color stability of flowable composite materials. AIM: The aim of this study was to evaluate the spectrophotometric color stability of three different flowable composite materials with respect to three different pH of saliva. MATERIALS AND METHODS: The study included 90 different samples...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27881347/-effect-of-rhynchophylline-on-behaviors-of-methamphetamine-dependent-zebrafish-and-the-mechanism
#9
Yi-Fei Chen, Ju Peng, Miao Fang, Yi Liu, Ling-Hui Nie, Zhi-Xian Mo, Ling-Ling Zhu
OBJECTIVE: To observe the effect of rhynchophylline on methamphetamine-dependent zebrafish and explore the possible mechanism. METHODS: Zebrafish were divided into control group, amphetamine group, low- (50 mg/kg) and high (100 mg/kg)-dose rhynchophylline groups, and ketamine (150 mg/kg) group. Conditioned place preference (CPP) was induced in zebrafish with methamphetamine, and the staying time in the drug box and the tracking map of the zebrafish were observed with Noldus Ethovision XT system...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27878365/-implantable-loop-recorders-of-the-reveal-family-medtronic
#10
Frederik Voss
This review explains the implantable loop recorders Medtronic Reveal XT and Medtronic Reveal LINQ. Technical specifications of the two devices are described in great detail. Additional tips for implantation as well as device programming are given including specific considerations of follow-up.
November 22, 2016: Herzschrittmachertherapie & Elektrophysiologie
https://www.readbyqxmd.com/read/27867058/application-of-strep-tactin-xt-for-affinity-purification-of-twin-strep-tagged-cb2-a-g-protein-coupled-cannabinoid-receptor
#11
Alexei Yeliseev, Lioudmila Zoubak, Thomas G M Schmidt
Human cannabinoid receptor CB2 belongs to the class A of G protein-coupled receptor (GPCR). High resolution structural studies of CB2 require milligram quantities of purified, structurally intact protein. Here we describe an efficient protocol for purification of this protein using the Twin-Strep-tag/Strep-Tactin XT system. To improve the affinity of interaction of the recombinant CB2 with the resin, the double repeat of the Strep-tag was attached either to the N- or C-terminus of CB2 via a short linker. The CB2 was isolated at high purity from dilute solutions containing high concentrations of detergents, glycerol and salts, by capturing onto the Strep-Tactin XT resin, and was eluted from the resin under mild conditions upon addition of biotin...
November 17, 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27862350/establishment-of-complete-blood-count-reference-intervals-for-chinese-preschoolers
#12
Guo-Cheng Wang, Na Li, Cui Niu, Wei-Bo Ma, Zhu-Liang Wang, Hao Guo, Li-Hong Han
BACKGROUND: Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS: Three thousand eight hundred and twenty-one blood specimens from children aged 4 months to 6 years were collected and analyzed. Complete blood counts were determined by Sysmex XT-4000i Automated Hematology Analyzer. The nonparametric 2...
November 15, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27862030/maintaining-accurate-long-term-sensing-ability-despite-significant-size-reduction-of-implantable-cardiac-monitors
#13
Julia Collin, Varnavas Varnavas, Wilko WEIßENBERGER, Raimund Erbel, Nico Reinsch
BACKGROUND: The Reveal LINQ™ implantable cardiac monitor (ICM) is obviously smaller than its precursor (the Reveal XT™, but little is known about its long-term safety. Here we investigated the long-term R-wave sensing reliability of the Reveal LINQ™ ICM. METHODS: We analyzed the sensing quality of the Reveal LIN Q™ ICM over time between March 2014 and January 2015. RESULTS: A total of 30 patients underwent Reveal LINQ™ implantation...
November 11, 2016: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/27849158/neurological-injury-in-intermediate-risk-transcatheter-aortic-valve-implantation
#14
Jonathon P Fanning, Allan J Wesley, Darren L Walters, Eamonn M Eeles, Adrian G Barnett, David G Platts, Andrew J Clarke, Andrew A Wong, Wendy E Strugnell, Cliona O'Sullivan, Oystein Tronstad, John F Fraser
BACKGROUND: The application of transcatheter aortic valve implantation (TAVI) to intermediate-risk patients is a controversial issue. Of concern, neurological injury in this group remains poorly defined. Among high-risk and inoperable patients, subclinical injury is reported on average in 75% undergoing the procedure. Although this attendant risk may be acceptable in higher-risk patients, it may not be so in those of lower risk. METHODS AND RESULTS: Forty patients undergoing TAVI with the Edwards SAPIEN-XT(™) prosthesis were prospectively studied...
November 15, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27848007/transcatheter-tricuspid-valve-in-valve-replacement-one-year-results-alternative-to-surgery-in-high-risk-patients
#15
João Mesquita, Rui Campante Teles, José Pedro Neves, João Abecasis, Pedro Carmo, João Brito, Miguel Abecasis, Manuel Sousa Almeida, Marisa Trabulo, Regina Ribeiras, Ricardo Seabra-Gomes, Miguel Mendes
Although rheumatic heart disease is becoming uncommon in industrialized countries, its global burden is still significant. We report the case of a 70-year-old male with rheumatic heart disease, who underwent 4 previous heart valve replacement surgeries, and presented to our hospital with refractory heart failure (NYHA functional class IV) due to severe stenosis of a previously implanted tricuspid bioprosthesis. The Heart Team deemed the patient as inoperable/high-risk for surgery. As an alternative, a transcatheter tricuspid valve-in-valve replacement was decided upon and later executed through the right femoral vein, with the insertion of an Edwards SAPIEN XT 29 no...
November 15, 2016: Heart and Vessels
https://www.readbyqxmd.com/read/27846458/predictive-factor-and-clinical-consequence-of-left-bundle-branch-block-after-a-transcatheter-aortic-valve-implantation
#16
Takahiko Nishiyama, Sho Tanosaki, Makoto Tanaka, Ryo Yanagisawa, Fumiaki Yashima, Takehiro Kimura, Takahide Arai, Hikaru Tsuruta, Mitsushige Murata, Yoshiyasu Aizawa, Takashi Kohno, Yuichiro Maekawa, Kentaro Hayashida, Seiji Takatsuki, Keiichi Fukuda
BACKGROUND: Atrioventricular conduction disturbances can develop after transcatherter aortic valve implantations (TAVIs) with balloon-expandable valves because the conduction system exists adjacent to the aortic valve. However, the clinical consequence of patients with new onset conduction disturbances is not clear. OBJECTIVE: This study aimed to assess the incidence and progress of new-onset conduction disturbances following TAVIs and the cardiac function evaluated by echocardiography...
November 9, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27843887/comparative-evaluation-and-influence-on-shear-bond-strength-of-incorporating-silver-zinc-oxide-and-titanium-dioxide-nanoparticles-in-orthodontic-adhesive
#17
Aileni Kaladhar Reddy, Prabhuraj B Kambalyal, Santosh R Patil, Mallikarjun Vankhre, Mohammed Yaser Ahmed Khan, Thamtam Ramana Kumar
OBJECTIVE: To investigate the influence of silver (Ag), zinc oxide (ZnO), and titanium dioxide (TiO2) nanoparticles on shear bond strength (SBS). MATERIALS AND METHODS: One hundred and twenty extracted premolars divided into four groups with thirty specimens in each group. Group 1 (control): brackets (American Orthodontics) were bonded with Transbond XT primer. Groups 2, 3, and 4: brackets (American Orthodontics) were bonded with adhesives incorporated with Ag, ZnO, and TiO2 nanoparticles in the concentration of 1...
October 2016: Journal of Orthodontic Science
https://www.readbyqxmd.com/read/27836147/effect-of-artificial-toothbrushing-and-water-storage-on-the-surface-roughness-and-micromechanical-properties-of-tooth-colored-cad-cam-materials
#18
Simon Flury, Elisabeth Diebold, Anne Peutzfeldt, Adrian Lussi
STATEMENT OF PROBLEM: Because of the different composition of resin-ceramic computer-aided design and computer-aided manufacturing (CAD-CAM) materials, their polishability and their micromechanical properties vary. Moreover, depending on the composition of the materials, their surface roughness and micromechanical properties are likely to change with time. PURPOSE: The purpose of this in vitro study was to investigate the effect of artificial toothbrushing and water storage on the surface roughness (Ra and Rz) and the micromechanical properties, surface hardness (Vickers [VHN]) and indentation modulus (EIT), of 5 different tooth-colored CAD-CAM materials when polished with 2 different polishing systems...
November 14, 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/27835070/symptoms-in-children-with-intermittent-exotropia-and-their-impact-on-health-related-quality-of-life
#19
Sarah R Hatt, David A Leske, Laura Liebermann, Jonathan M Holmes
PURPOSE: In childhood intermittent XT, the frequency and type of symptoms have not been rigorously studied. We aimed to identify specific symptoms in children with intermittent XT, their frequency, and effects on health-related quality of life (HRQOL). METHODS: 35 children (5-13 years) with intermittent XT without previous surgery were enrolled in a prospective cohort study. Specific symptoms were identified from a previous study involving child and parent interviews and formulated as a 22-item symptom questionnaire...
December 2016: Strabismus
https://www.readbyqxmd.com/read/27832846/pre-existing-right-bundle-branch-block%C3%A2-increases-risk-for-death-after-transcatheter-aortic-valve-replacement-with-a-balloon-expandable-valve
#20
Yusuke Watanabe, Ken Kozuma, Hirofumi Hioki, Hideyuki Kawashima, Yugo Nara, Akihisa Kataoka, Fukuko Nagura, Makoto Nakashima, Shinichi Shirai, Norio Tada, Motoharu Araki, Kensuke Takagi, Futoshi Yamanaka, Masanori Yamamoto, Kentaro Hayashida
OBJECTIVES: The aim of this study was to determine the impact of pre-existing right bundle branch block (RBBB) on clinical outcomes after transcatheter aortic valve replacement (TAVR). BACKGROUND: The impact of pre-existing RBBB on clinical outcomes after TAVR is unknown. METHODS: Between October 2013 and August 2015, 749 patients undergoing TAVR using the Edwards SAPIEN XT prosthesis were prospectively enrolled in the OCEAN-TAVI (Optimized Transcatheter Valvular Intervention) registry from 8 Japanese centers...
November 14, 2016: JACC. Cardiovascular Interventions
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