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https://www.readbyqxmd.com/read/28448897/a-performance-evaluation-of-nextera-xt-and-kapa-hyperplus-for-rapid-illumina-library-preparation-of-long-range-mitogenome-amplicons
#1
Joseph D Ring, Kimberly Sturk-Andreaggi, Michelle A Peck, Charla Marshall
Next-generation sequencing (NGS) facilitates the rapid and high-throughput generation of human mitochondrial genome (mitogenome) data to build population and reference databases for forensic comparisons. To this end, long-range amplification provides an effective method of target enrichment that is amenable to library preparation assays employing DNA fragmentation. This study compared the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) and the KAPA HyperPlus Library Preparation Kit (Kapa Biosystems, Wilmington, MA) for enzymatic fragmentation and indexing of ∼8500bp mitogenome amplicons for Illumina sequencing...
April 5, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28448581/the-performance-of-bd-facspresto%C3%A2-for-cd4-t-cell-count-cd4-and-hemoglobin-concentration-test-in-ethiopia
#2
Gebremedhin Gebremicael, Yohanes Belay, Fitsum Girma, Yemane Abreha, Atsbeha Gebreegziabxier, Simret Tesfaye, Zelalem Messele, Yibeltal Assefa, Bahrie Bellete, Desta Kassa, Lara Vojnov
INTRODUCTION: In Ethiopia, CD4+ T-cell counting is still required for all patients at baseline before antiretroviral therapy (ART) and to determine eligibility and follow-up of opportunistic infection prophylaxis. However, access to CD4+ T cell count in rural health facilities remains a major challenge in Ethiopia like other resource-limited settings. METHODOLOGY: Both capillary and venous blood was drawn from each of 325 study participant recruited in Addis Ababa and surroundings...
2017: PloS One
https://www.readbyqxmd.com/read/28448161/peaches-for-lunch-creating-and-using-visual-variables
#3
Elizabeth Cartwright, Adam Clegg
In this article, I describe the process of systematically including non-verbal data in medical anthropology research. I demonstrate the process of visualizing and coding videotaped moments of life and show how we can analyze what is being done along with what is being said. I ground my discussion in toddler language socialization and then expand my observations to the realm of language pathologies. Aphasia from strokes, speech difficulties in neurologically based illnesses like Lou Gehrig's disease, and the variety of communication challenges that face those on the autism spectrum can all be studied in interesting ways by including precise descriptions of non-verbal actions...
April 27, 2017: Medical Anthropology
https://www.readbyqxmd.com/read/28444177/single-centre-experience-with-transapical-transcatheter-mitral-valve-implantation%C3%A2
#4
Gry Dahle, Kjell-Arne Rein, Arnt E Fiane
OBJECTIVES: A transcatheter heart valve technique can be used in failed mitral valve repairs with annuloplasty rings, deterioriated bioprostheses and in mitral annular calcification, all serving as 'docking stations' for balloon-expandable valves. Specially designed transcatheter mitral valve platforms are used in ongoing studies for native mitral valve regurgitation. We present our single centre experience with transcatheter mitral valve implantation-transapical approach procedures in eleven patients...
April 21, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28444018/effect-of-nanotechnology-in-self-etch-bonding-systems-on-the-shear-bond-strength-of-stainless-steel-orthodontic-brackets
#5
Shaza M Hammad, Noha El-Wassefy, Ahmed Maher, Shafik M Fawakerji
OBJECTIVE: To evaluate the effect of silica dioxide (SiO2) nanofillers in different bonding systems on shear bond strength (SBS) and mode of failure of orthodontic brackets at two experimental times. METHODS: Ninety-six intact premolars were divided into four groups: A) Conventional acid-etch and primer Transbond XT; B) Transbond Plus self-etch primer; and two self-etch bonding systems reinforced with silica dioxide nanofiller at different concentrations: C) Futurabond DC at 1%; D) Optibond All-in-One at 7%...
February 2017: Dental Press Journal of Orthodontics
https://www.readbyqxmd.com/read/28420223/clinical-and-molecular-characterization-of-patients-with-fructose-1-6-bisphosphatase-deficiency
#6
Niu Li, Guoying Chang, Yufei Xu, Yu Ding, Guoqiang Li, Tingting Yu, Yanrong Qing, Juan Li, Yiping Shen, Jian Wang, Xiumin Wang
Fructose-1,6-bisphosphatase (FBPase) deficiency is a rare, autosomal recessive inherited disease caused by the mutation of the FBP1 gene, the incidence is estimated to be between 1/350,000 and 1/900,000. The symptoms of affected individuals are non-specific and are easily confused with other metabolic disorders. The present study describes the clinical features of four Chinese pediatric patients who presented with hypoglycemia, hyperlactacidemia, metabolic acidosis, and hyperuricemia. Targeted-next generation sequencing using the Agilent SureSelect XT Inherited Disease Panel was used to screen for causal variants in the genome, and the clinically-relevant variants were subsequently verified using Sanger sequencing...
April 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28418649/nature-s-selection-of-geranyl-group-as-a-trna-modification-the-effects-of-chain-length-on-base-pairing-specificity
#7
Phensinee Haruehanroengra, Sweta Vangaveti, Srivathsan V Ranganathan, Rui Wang, Alan Chen, Jia Sheng
The recently discovered geranyl modification on the 2-thio position of wobble U34 residues in tRNA(Glu), tRNA(Lys), and tRNA(Gln) in several bacteria has been found to enhance the U:G pairing specificity and reduce the frameshifting error during translation. It is a fundamentally interesting question why nature chose a C10 terpene group in tRNA systems. In this study, we explore the significance of the terpene length on base-paring stability and specificity using a series of 2-thiouridine analogues containing different lengths of carbon chains, namely, methyl- (C1), dimethylallyl- (C5), and farnesyl-modified (C15) 2-thiothymidines in a DNA duplex...
April 18, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28412951/understanding-discharge-communication-behaviours-in-a-pediatric-emergency-care-context-a-mixed-methods-observation-study-protocol
#8
Janet A Curran, Andrea Bishop, Amy Plint, Shannon MacPhee, Roger Zemek, Jill Chorney, Mona Jabbour, Stephen Porter, Scott Sawyer
BACKGROUND: One of the most important transitions in the continuum of care for children is discharge to home. Optimal discharge communication between healthcare providers and caregivers (e.g., parents or other guardians) who present to the emergency department (ED) with their children is not well understood. The lack of policies and considerable variation in practice regarding discharge communication in pediatric EDs pose a quality and safety risk for children and their parents. METHODS: The aim of this mixed methods study is to better understand the process and structure of discharge communication in a pediatric ED context to contribute to the design and development of discharge communication interventions...
April 17, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28411108/causes-and-temporal-trends-in-procedural-deaths-after-transcatheter-aortic-valve-implantation
#9
Zaven Terzian, Marina Urena, Dominique Himbert, Constance Gardy-Verdonk, Bernard Iung, Claire Bouleti, Eric Brochet, Walid Ghodbane, Jean-Pol Depoix, Patrick Nataf, Alec Vahanian
BACKGROUND: The causes of procedural deaths after transcatheter aortic valve implantation (TAVI) have been scarcely detailed. AIMS: To assess these causes and their temporal trends since the beginning of the TAVI era. METHODS: From October 2006 to April 2014, 601 consecutive high-risk/inoperable patients with severe aortic stenosis underwent TAVI using the Edwards SAPIEN or SAPIEN XT or the Medtronic CoreValve. The transfemoral route was the default approach; the transapical or left subclavian approaches were alternative options...
April 11, 2017: Archives of Cardiovascular Diseases
https://www.readbyqxmd.com/read/28410636/stent-and-leaflet-stresses-in-29-mm-second-generation-balloon-expandable-transcatheter-aortic-valve
#10
Yue Xuan, Kapil Krishnan, Jian Ye, Danny Dvir, Julius M Guccione, Liang Ge, Elaine E Tseng
BACKGROUND: Equipoise of transcatheter aortic valve replacement with surgical aortic valve replacement in intermediate-risk patients has been demonstrated. As transcatheter aortic valve replacement usage expands, questions regarding long-term durability become paramount. Valve design impacts durability with regions of increased leaflet stress being vulnerable to early failure. However, transcatheter aortic valve (TAV) leaflet stresses are unknown. The objective of this study was to determine stent and leaflet stresses of second-generation balloon-expandable TAV...
April 12, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28403360/influence-of-resin-modified-glass-ionomer-and-topical-fluoride-on-levels-of-streptococcus-mutans-in-saliva-and-biofilm-adjacent-to-metallic-brackets
#11
Marcela Cristina Damião Andrucioli, Gisele Faria, Paulo Nelson-Filho, Fábio Lourenço Romano, Mírian Aiko Nakane Matsumoto
Decalcification of enamel during fixed orthodontic appliance treatment remains a problem. White spot lesions are observed in nearly 50% of patients undergoing orthodontic treatment. The use of fluoride-containing orthodontic materials has shown inconclusive results on their ability to reduce decalcification. The aims of this investigation were to compare the levels of Streptococcus mutans (SM) in saliva and biofilm adjacent to orthodontic brackets retained with a resin-modified glass ionomer cement (RMGIC) (Fuji ORTHO LC) and a light cured composite resin (Transbond XT), and to analyze the influence of topical application of the 1...
March 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28392716/er-yag-pre-treatment-for-bonding-of-orthodontic-bracket-1-year-of-in-vitro-treatment
#12
Rudys Rodolfo de Jesus Tavarez, Gisele Lima Bezerra, Karla Janilee de Souza Penha, Carlos Rocha Gomes Torres, Leily Macedo Firoozmand
OBJECTIVE: The aim of this study was to evaluate in vitro bond strength of metal brackets bonded with: total etch, total etch with erbium: yttrium aluminum garnet laser (Er:YAG) and self-etching adhesive systems, submitted to thermal-mechanical cycling, simulating 1 year of orthodontic treatment. MATERIALS AND METHODS: For the study, 80 bovine incisors were randomly divided into 3 experimental groups (n=16 each): XT- acid etching + Transbond XT, XT/Er:YAG- Transbond XT associated with Er:YAG laser irradiation (λ=2...
2017: Clinical, Cosmetic and Investigational Dentistry
https://www.readbyqxmd.com/read/28391002/paravalvular-regurgitation-after-transcatheter-aortic-valve-replacement-comparing-transthoracic-versus-transesophageal-echocardiographic-guidance
#13
Salim S Hayek, Frank E Corrigan, Jose F Condado, Shuang Lin, Sharon Howell, James P MacNamara, Shuai Zheng, Patricia Keegan, Vinod Thourani, Vasilis C Babaliaros, Stamatios Lerakis
BACKGROUND: Transcatheter aortic valve replacement (TAVR) is increasingly being performed in cardiac catheterization laboratories using transthoracic echocardiography (TTE) to guide valve deployment. The risk of paravalvular regurgitation (PVR) remains a concern. METHODS: We retrospectively reviewed 454 consecutive patients (mean age, 82 ± 8; 58% male) who underwent transfemoral TAVR at Emory Healthcare from 2007 to 2014. Two hundred thirty-four patients underwent TAVR in the cardiac catheterization laboratory with TTE guidance (TTE-TAVR; mean Society of Thoracic Surgeons score, 10%), while 220 patients underwent the procedure in the hybrid operating room with transesophageal echocardiography (TEE) guidance (TEE-TAVR; mean Society of Thoracic Surgeons score, 11%)...
April 6, 2017: Journal of the American Society of Echocardiography
https://www.readbyqxmd.com/read/28389467/aorto-right-ventricular-fistula-following-transcatheter-aortic-valve-implantation-using-a-29-mm-sapien-xt-valve
#14
Kazuki Hagiwara, Naritatsu Saito, Kazuhiro Yamazaki, Takeshi Kimura
An 83-year-old man with severe symptomatic aortic valve stenosis underwent transcatheter aortic valve implantation (TAVI) using a 29 mm SAPIEN XT valve. He was haemodynamically stable, but developed haemolytic anaemia. Transthoracic echocardiography conducted 7 days after TAVI revealed an abnormal continuous flow from the right sinus of Valsalva adjacent to the implanted valve to the right ventricle. The amount of shunt flow was considered small and the patient was managed with diuretics successfully. He was discharged from the hospital 35 days after TAVI...
April 7, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28386279/effect-of-rebonding-on-the-bond-strength-of-orthodontic-tubes-a-comparison-of-light-cure-adhesive-and-resin-modified-glass-ionomer-cement-in-vitro
#15
Monika Aleksiejunaite, Antanas Sidlauskas, Arunas Vasiliauskas
The purpose of this study was to determine the impact of different enamel preparation procedures and compare light cure composite (LCC) and resin-modified glass ionomer (RMGI) on the bond strength of orthodontic metal tubes rebonded to the enamel. Twenty human molars were divided into two groups (n = 10). Tubes were bonded using LCC (Transbond XT) in group 1 and RMGI (Fuji Ortho LC) in group 2. The tubes in each group were bonded following manufacturers' instructions (experiment I) and then debonded using testing machine...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/28380777/design-and-optimization-of-32-core-rod-trench-assisted-square-lattice-structured-single-mode-multi-core-fiber
#16
Xueqin Xie, Jiajing Tu, Xian Zhou, Keping Long, Kunimasa Saitoh
We propose and design a kind of heterogeneous rod-assisted and trench-assisted multi-core fiber (Hetero-RA-TA-MCF) with 32 cores arranged in square-lattice structure (SLS), and then we introduce the design method for Hetero-RA-TA-MCF. Simulation results show that the Hetero-RA-TA-32-Core-Fiber achieves average effective area (A<sub>eff</sub>) of about 74 μm<sup>2</sup>, low crosstalk (XT) of about -31 dB/100km, threshold value of bending radius (R<sub>pk</sub>) of 7.0 cm, relative core multiplicity factor (RCMF) of 8...
March 6, 2017: Optics Express
https://www.readbyqxmd.com/read/28371609/trastuzumab-and-pertuzumab-plant-biosimilars-modification-of-asn297-linked-glycan-of-the-mabs-produced-in-a-plant-with-fucosyltransferase-and-xylosyltransferase-gene-knockouts
#17
T V Komarova, E V Sheshukova, E N Kosobokova, M V Serebryakova, V S Kosorukov, V N Tashlitsky, Y L Dorokhov
Plant biosimilars of anticancer therapeutic antibodies are of interest not only because of the prospects of their practical use, but also as an instrument and object for study of plant protein glycosylation. In this work, we first designed a pertuzumab plant biosimilar (PPB) and investigated the composition of its Asn297-linked glycan in comparison with trastuzumab plant biosimilar (TPB). Both biosimilars were produced in wild-type (WT) Nicotiana benthamiana plant (PPB-WT and TPB-WT) and transgenic ΔXTFT N...
April 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28367042/a-valid-indication-and-the-effect-of-bilateral-inferior-oblique-transposition-on-recurrent-or-consecutive-horizontal-deviation-in-infantile-strabismus
#18
Suk-Gyu Ha, Gun-Hoo Na, Seung-Hyun Kim
PURPOSE: To evaluate the effects of bilateral inferior oblique transposition (BIOT) on horizontal deviation from primary position among patients with bilateral dissociated vertical deviation (DVD) associated with inferior oblique overaction (IOOA) in infantile strabismus. METHODS: Retrospective chart review was conducted among 19 patients with infantile strabismus. All patients had DVD and IOOA with consecutive or recurrent horizontal deviation and underwent modified BIOT surgery...
April 2017: Korean Journal of Ophthalmology: KJO
https://www.readbyqxmd.com/read/28364889/streptococcus-mutans-forms-xylitol-resistant-biofilm-on-excess-adhesive-flash-in-novel-ex-vivo-orthodontic-bracket-model
#19
Cindy S F Ho, Yue Ming, Kelvin W C Foong, Vinicius Rosa, Truong Thuyen, Chaminda J Seneviratne
INTRODUCTION: During orthodontic bonding procedures, excess adhesive is invariably left on the tooth surface at the interface between the bracket and the enamel junction; it is called excess adhesive flash (EAF). We comparatively evaluated the biofilm formation of Streptococcus mutans on EAF produced by 2 adhesives and examined the therapeutic efficacy of xylitol on S mutans formed on EAF. METHODS: First, we investigated the biofilm formation of S mutans on 3 orthodontic bracket types: stainless steel preadjusted edgewise, ceramic preadjusted edgewise, and stainless steel self-ligating...
April 2017: American Journal of Orthodontics and Dentofacial Orthopedics
https://www.readbyqxmd.com/read/28357625/a-case-of-double-stent-implantation-for-left-main-coronary-artery-occlusion-in-transcatheter-aortic-valve-implantation-using-sapien-xt-device
#20
Masaki Ito, Norio Tada, Tatsushi Ootomo, Naoto Inoue
Transcatheter aortic valve implantation is an established alternative and less invasive procedure to replace heart valves in symptomatic aortic stenosis patients; however, severe, life-threatening complications still exist. Coronary artery occlusion is a primary complication. We report a case of left main coronary artery occlusion after transcatheter aortic valve replacement, which was ameliorated using a double stent implantation technique.
March 29, 2017: Cardiovascular Intervention and Therapeutics
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